Small G protein signalling modulator 2 (SGSM2) is involved in oestrogen receptor-positive breast cancer metastasis through enhancement of migratory cell adhesion via interaction with E-cadherin.

IF 3.3 3区生物学 Q3 CELL BIOLOGY

Cell Adhesion & Migration Pub Date : 2019-12-01 Epub Date: 2019-02-11 DOI:10.1080/19336918.2019.1568139

Juo-Han Lin, Wen-Jui Lee, Han-Chung Wu, Chih-Hsiung Wu, Li-Ching Chen, Chi-Cheng Huang, Hang-Lung Chang, Tzu-Chun Cheng, Hui-Wen Chang, Chi-Tang Ho, Shih-Hsin Tu, Yuan-Soon Ho

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引用次数: 9

Abstract

The function of small G protein signalling modulators (SGSM1/2/3) in cancer remains unknown. Our findings demonstrated that SGSM2 is a plasma membrane protein that strongly interacted with E-cadherin/β-catenin. SGSM2 downregulation enhanced the phosphorylation of focal adhesion kinase (FAK; Y576/577), decreased the expression of epithelial markers such as E-cadherin, β-catenin, and Paxillin, and increased the expression of Snail and Twist-1, which reduced cell adhesion and promoted cancer cell migration. Oestrogen and fibronectin treatment was found to promote the colocalization of SGSM2 at the leading edge with phospho-FAK (Y397). The BioGRID database showed that SGSM2 potentially interacts with cytoskeleton remodelling and cell-cell junction proteins. These evidences suggest that SGSM2 plays a role in modulating cell adhesion and cytoskeleton dynamics during cancer migration.

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小G蛋白信号调节因子2 (SGSM2)通过与E-cadherin相互作用增强迁移细胞粘附，参与雌激素受体阳性乳腺癌转移。

小G蛋白信号调节剂(SGSM1/2/3)在癌症中的作用尚不清楚。我们的研究结果表明，SGSM2是一种与E-cadherin/β-catenin强烈相互作用的质膜蛋白。SGSM2下调可增强局灶黏附激酶(FAK)的磷酸化;Y576/577)，降低上皮标志物E-cadherin、β-catenin、Paxillin的表达，增加Snail、Twist-1的表达，降低细胞粘附，促进癌细胞迁移。雌激素和纤维连接蛋白治疗可促进SGSM2与磷酸化- fak (Y397)的共定位。BioGRID数据库显示，SGSM2可能与细胞骨架重塑和细胞-细胞连接蛋白相互作用。这些证据表明，SGSM2在肿瘤迁移过程中调节细胞粘附和细胞骨架动力学中起作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Adhesion & Migration CELL BIOLOGY-

CiteScore

6.40

自引率

0.00%

发文量

审稿时长

53 weeks

期刊介绍： Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field. Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.