Neutrophils as a source of branched-chain, aromatic and positively charged free amino acids.

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

ACS Applied Electronic Materials Pub Date : 2019-12-01 Epub Date: 2018-10-29 DOI:10.1080/19336918.2018.1540903

Svetlana I Galkina, Natalia V Fedorova, Alexander L Ksenofontov, Vladimir I Stadnichuk, Ludmila A Baratova, Galina F Sud'Ina

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引用次数: 11

Abstract

Neutrophils release branched-chain (valine, isoleucine, leucine), aromatic (tyrosine, phenylalanine) and positively charged free amino acids (arginine, ornithine, lysine, hydroxylysine, histidine) when adhere and spread onto fibronectin. In the presence of agents that impair cell spreading or adhesion (cytochalasin D, fMLP, nonadhesive substrate), neutrophils release the same amino acids, except for a sharp decrease in hydroxylysine and an increase in phenylalanine, indicating their special connection with cell adhesion. Plasma of patients with diabetes is characterized by an increased content of branched-chain and aromatic amino acids and a reduced ratio of arginine/ornithine compared to healthy human plasma. Our data showed that the secretion of neutrophils, regardless of their adhesion state, can contribute to this shift in the amino acid content. Abbreviations: BCAAs: branched-chain amino acids; Е2: 17β-estradiol; LPS: lipopolysaccharide from Salmonella enterica serovar Typhimurium; fMLP: N-formylmethionyl-leucyl-phenylalanine.

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中性粒细胞是支链、芳香和带正电的游离氨基酸的来源。

中性粒细胞粘附并扩散到纤维连接蛋白上时，释放支链(缬氨酸、异亮氨酸、亮氨酸)、芳香(酪氨酸、苯丙氨酸)和带正电的游离氨基酸(精氨酸、鸟氨酸、赖氨酸、羟赖氨酸、组氨酸)。当存在损害细胞扩散或粘附的物质(细胞松弛素D, fMLP，非粘附底物)时，中性粒细胞释放相同的氨基酸，除了羟基赖氨酸急剧减少和苯丙氨酸增加，表明它们与细胞粘附的特殊联系。与健康人血浆相比，糖尿病患者血浆的特点是支链氨基酸和芳香氨基酸含量增加，精氨酸/鸟氨酸比值降低。我们的数据表明，中性粒细胞的分泌，无论其粘附状态如何，都可以促进氨基酸含量的这种转变。BCAAs:支链氨基酸;Е2:17β雌二醇;LPS:取自肠炎沙门氏菌血清型鼠伤寒沙门氏菌的脂多糖;fMLP: N-formylmethionyl-leucyl-phenylalanine。

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来源期刊

ACS Applied Electronic Materials Multiple-

CiteScore

7.20

自引率

4.30%

发文量

567