Continuous, label-free, 96-well-based determination of cell migration using confluence measurement.

IF 3.3 3区生物学 Q3 CELL BIOLOGY

Cell Adhesion & Migration Pub Date : 2019-12-01 Epub Date: 2018-10-08 DOI:10.1080/19336918.2018.1526612

Christian Mayr, Marlena Beyreis, Heidemarie Dobias, Martin Gaisberger, Julia Fuchs, Martin Pichler, Markus Ritter, Martin Jakab, Katharina Helm, Daniel Neureiter, Tobias Kiesslich

{"title":"Continuous, label-free, 96-well-based determination of cell migration using confluence measurement.","authors":"Christian Mayr, Marlena Beyreis, Heidemarie Dobias, Martin Gaisberger, Julia Fuchs, Martin Pichler, Markus Ritter, Martin Jakab, Katharina Helm, Daniel Neureiter, Tobias Kiesslich","doi":"10.1080/19336918.2018.1526612","DOIUrl":null,"url":null,"abstract":"<p><p>Cellular migration is essential in diverse physiological and pathophysiological processes. Here, we present a protocol for quantitative analysis of migration using confluence detection allowing continuous, non-endpoint measurement with minimal hands-on time under cell incubator conditions. Applicability was tested using substances which enhance (EGF) or inhibit (cytochalasin D, ouabain) migration. Using a gap-closure assay we demonstrate that automated confluence detection monitors cellular migration in the 96-well microplate format. Quantification by % confluence, % cell free-area or % confluence in cell-free area against time, allows detailed analysis of cellular migration. The study describes a practicable approach for continuous, non-endpoint measurement of migration in 96-well microplates and for detailed data analysis, which allows for medium/high-throughput analysis of cellular migration in vitro.</p>","PeriodicalId":9680,"journal":{"name":"Cell Adhesion & Migration","volume":"13 1","pages":"76-82"},"PeriodicalIF":3.3000,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336918.2018.1526612","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell Adhesion & Migration","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/19336918.2018.1526612","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2018/10/8 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}

引用次数: 1

Abstract

Cellular migration is essential in diverse physiological and pathophysiological processes. Here, we present a protocol for quantitative analysis of migration using confluence detection allowing continuous, non-endpoint measurement with minimal hands-on time under cell incubator conditions. Applicability was tested using substances which enhance (EGF) or inhibit (cytochalasin D, ouabain) migration. Using a gap-closure assay we demonstrate that automated confluence detection monitors cellular migration in the 96-well microplate format. Quantification by % confluence, % cell free-area or % confluence in cell-free area against time, allows detailed analysis of cellular migration. The study describes a practicable approach for continuous, non-endpoint measurement of migration in 96-well microplates and for detailed data analysis, which allows for medium/high-throughput analysis of cellular migration in vitro.

Abstract Image

查看原文本刊更多论文

连续，无标记，96孔为基础的测定细胞迁移使用合流测量。

细胞迁移在多种生理和病理生理过程中是必不可少的。在这里，我们提出了一种使用合流检测进行迁移定量分析的方案，允许在细胞培养条件下用最少的动手时间进行连续的非终点测量。使用增强(EGF)或抑制(细胞松弛素D，瓦巴因)迁移的物质来测试适用性。使用间隙关闭试验，我们证明了自动汇合检测监测细胞迁移在96孔微孔板格式。定量通过%汇合，%细胞自由面积或细胞自由面积随时间的%汇合，允许细胞迁移的详细分析。该研究描述了一种可行的方法，可以在96孔微孔板上连续、无终点地测量迁移，并进行详细的数据分析，从而可以在体外对细胞迁移进行中/高通量分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Cell Adhesion & Migration CELL BIOLOGY-

CiteScore

6.40

自引率

0.00%

发文量

审稿时长

53 weeks

期刊介绍： Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field. Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.