Access microbiology最新文献

{"title":"Meta-analysis of functional genomics studies reveals conserved cellular pathways required by viruses of pandemic concern.","authors":"Aaron M Brice, Alessandro T Caputo, Cameron R Stewart","doi":"10.1099/acmi.0.001167.v3","DOIUrl":"10.1099/acmi.0.001167.v3","url":null,"abstract":"<p><p>The coronavirus disease 2019 pandemic illustrated the need to develop medical countermeasures against emerging infectious diseases. As viruses rely on cellular machinery for replication, host-directed antivirals (HDAs) may complement conventional antiviral strategies in a manner that offers broad-spectrum efficacy, including against novel viruses, and a potentially higher barrier to viral escape. Despite their potential, HDAs are under-represented as therapeutics, partly due to a lack of consensus on druggable host targets. To address this, we have performed a meta-analysis of 62 functional genomics studies involving viral families of pandemic concern, including <i>Arenaviridae</i>, <i>Coronaviridae</i>, <i>Filoviridae</i>, <i>Flaviviridae</i>, <i>Orthomyxoviridae</i>, <i>Paramyxoviridae</i>, <i>Phenuiviridae</i>, <i>Picornaviridae</i>, <i>Poxviridae</i> and <i>Togaviridae</i>. Using a RRA and protein-protein interaction network approach, host factors and cellular processes required by multiple virus families were identified, including the V-type ATPase complex, glycosaminoglycan synthesis, Golgi trafficking and endoplasmic reticulum membrane protein insertion. These pathways include those with known relevance to infection by some viruses while providing novel insights into the life cycles of others. Therapeutic targeting of these top ranked host factors is also discussed, with several already possessing small-molecule inhibitors, highlighting their therapeutic potential. Antivirals are an essential component of medical countermeasures against viral disease and fulfil a complementary role to vaccines. Importantly, they may provide the only therapeutic option for pathogens lacking effective vaccines or for individuals unable to be vaccinated. This analysis furthers our understanding of the virus-host interface and nominates cellular targets for the future development of HDAs as medical countermeasures for pandemic resilience.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"8 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13281952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148298332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of enteroviruses in patients with acute respiratory infection and influenza-like illness in the Central African Republic revealed mainly polioviruses and other enteric enterovirus types.","authors":"Joël W Doté, Giscard Francis Komoyo, Bertille Ndombari-Beta, Marie-Line Joffret, Mohand Ait-Ahmed, Emmanuel Nakouné, Maël Bessaud, Ionela Gouandjika-Vasilache","doi":"10.1099/acmi.0.001198.v3","DOIUrl":"10.1099/acmi.0.001198.v3","url":null,"abstract":"<p><p>Enteroviruses (EVs) that infect humans cause a broad range of pathologies, notably neurological, respiratory, cutaneous and enteric diseases, leading to morbidity and mortality, especially in young children. These pathologies particularly affect countries in Asia and Sub-Saharan Africa. In the last decades, some EVs with a marked respiratory tropism have emerged as a source of acute respiratory illnesses: EV-D68 has been associated with outbreaks of severe respiratory distress and acute myelitis since 2014, while several respiratory genotypes belonging to the species <i>Enterovirus coxsackiepol</i> were discovered in the 2010s. Very little is known about the latter, and no data exist on their circulation or clinical impact in the Central African Republic. In an attempt to bridge this gap, a retrospective study was carried out on nasopharyngeal samples collected as part of influenza surveillance at five sentinel sites in the Central African Republic in 2019. A total of 178 samples negative for influenza viruses were pooled and screened for the detection of EVs (rhinoviruses excluded). A positive result was obtained for 17 out of 20 pools. Genomic amplification was attempted by using generic primers against EVs (rhinoviruses excluded), and the virus genomes were partly sequenced by Illumina technology. A total of 46 contigs covered the VP1 region. The corresponding viruses belonged to several virus types of three species: <i>E. coxsackiepol</i> (14/26, 58.3%), <i>Enterovirus betacoxsackie</i> (9/26, 34.6%) and <i>Enterovirus alphacoxsackie</i> (3/26, 11.5%). Unexpectedly, no respiratory virus types (such as EV-D68, EV-C104, EV-C105, EV-C109, EV-C117 or EV-C118) were detected. Of note, polioviruses from vaccine origin were detected in eight pools (40.0%), supporting the notion that respiratory specimens may serve as a source for poliovirus re-introduction after eradication. In conclusion, we did not detect any respiratory EVs, a finding that most likely reflects a low baseline prevalence of these viruses during inter-epidemic periods. Moreover, we confirmed that several EV types, whose primary replication site is the gastrointestinal tract, are also capable of replicating in the oropharynx, polioviruses included, which constitutes a concern for mitigating the risk of poliovirus re-introduction in the post-eradication era.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"8 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13286286/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148311047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome sequencing of clinical methicillin-resistant Staphylococcus aureus isolates across hospitals in Mwanza, Tanzania.","authors":"Vitus Silago, Lawrence Mapunda, Prisca Damiano, Katarina Oravcova, Louise Matthews, Benson R Kidenya, Stephen E Mshana, Jeremiah Seni, Heike Claus","doi":"10.1099/acmi.0.001127.v5","DOIUrl":"10.1099/acmi.0.001127.v5","url":null,"abstract":"<p><p>Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is a leading cause of both healthcare-associated and community-acquired infections, characterized by limited treatment options and substantial morbidity and mortality, particularly in low- and middle-income countries such as Tanzania. However, genomic data, which provides valuable insights into the pathogen's genetic landscape, remains scarce. This study utilized whole-genome sequencing (WGS) to examine local molecular epidemiological profiles of 14 MRSA isolates isolated from blood, urine and pus samples during (<i>n</i>=6; June 2019-June 2020) and after (<i>n</i>=8; March-August 2023) the implementation of the National Action Plan on Antimicrobial Resistance in Mwanza, Tanzania. All isolates belonged to sequence type 8 (ST8), and 92.9% (13/14) were spa type <i>t</i>1476. Phenotypic and genotypic antimicrobial resistance results were consistent for all antibiotics tested with the exception of tetracycline. Pairwise SNP analysis revealed genetic diversity, with SNP differences ranging from 0 to 99. Maximum likelihood phylogeny analysis based on SNP data identified two closely related MRSA pairs, one from multiple wards while the other confined within the same ward in the same hospital. In conclusion, WGS revealed that all MRSA isolates belonged to ST8 with substantial genetic diversity and phylogenetic analysis revealed two closely related MRSA pairs, suggesting a transmission event, acquisition from a shared source or circulation of a closely related lineage. These findings highlight the importance of genomic surveillance for uncovering MRSA transmission patterns and guiding infection prevention efforts.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"8 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13262600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148255334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Type VI secretion system-mediated bacterial antagonism in the classroom.","authors":"Alejandro Tejada-Arranz, Peter Ringel, Lin Lin, Marek Basler","doi":"10.1099/acmi.0.001128.v3","DOIUrl":"10.1099/acmi.0.001128.v3","url":null,"abstract":"<p><p>The study of bacterial antagonism, and specifically type VI secretion system (T6SS)-mediated antagonism, has gained momentum over the last decade in research laboratories across the world. The T6SS is a molecular machine that mediates interbacterial antagonism, as well as interactions with the host in the case of pathogenic or commensal organisms. Here, we describe a set of protocols and materials that can be used as resources to develop practical courses to study the assembly and firing of this machinery. These resources help train students on basic molecular biology techniques and secretion and competition assays, as well as fluorescence microscopy. The full set of activities is appropriate for students at the level of second- to third-year students in the Bachelor of Biology and can be carried out in 4 days requiring minimal specialized equipment. This course allows the students to familiarize themselves with standard microbiology laboratory approaches and learn how to study the function of a complex molecular machinery.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"8 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13274762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148280832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of culture plates demonstrating anti-Staphylococcus aureus activity of Penicillium sp. and their use as teaching materials.","authors":"Tomoe Ichikawa, Yoshio Ishibashi","doi":"10.1099/acmi.0.001129.v3","DOIUrl":"10.1099/acmi.0.001129.v3","url":null,"abstract":"<p><p>Penicillin is recognized as one of the world's first antibiotics, originally derived from <i>Penicillium</i> sp. However, few students have directly observed the antibacterial activity of <i>Penicillium</i> sp. against bacterial pathogens. Therefore, we hypothesized that observing this phenomenon could enhance the interest of pharmacy students in micro-organisms and antibiotics. First, we selected a <i>Penicillium</i> strain exhibiting strong antibacterial activity and established a reproducible method to prepare culture plates showing clear inhibition of <i>Staphylococcus aureus</i> growth. During a microbiology practical course, students observed these prepared plates, and a questionnaire survey of university students was conducted before and after the session. Students were asked whether observing this antibacterial activity increased their interest in antibiotics and aided their retention of knowledge about antibiotics. Over 80% of respondents answered favourably, suggesting that incorporating images of plates showing <i>Penicillium</i>-mediated antibacterial activity into teaching would improve students' interest in microbiology and antibiotics.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"8 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13262599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148255342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Burden of extended-spectrum beta-lactamase-producing Enterobacteriaceae among cancer patients in Africa: a systematic review and meta-analysis.","authors":"Henry Zamarano, Benson Musinguzi, Muzafaru Twinomujuni, Catherine Khakasa, Vicent Mwesigye, Ivan Muhwezi, Edgar Mugema Mulogo, Deborah Natumanya, Simon Kawuma, Patrick Orikiriza, Jacob Stanley Iramiot","doi":"10.1099/acmi.0.001120.v4","DOIUrl":"10.1099/acmi.0.001120.v4","url":null,"abstract":"<p><p><b>Background.</b> Extended-spectrum beta-lactamase-producing <i>Enterobacteriaceae</i> (ESBL-P<i>E</i>) exacerbate infections in cancer patients in settings where antimicrobial resistance threatens health outcomes. This study estimated the prevalence of ESBL-P<i>E</i> among cancer patients in Africa from 1 January 2010 to 31 December 2024. <b>Methods.</b> We searched PubMed, Embase, Web of Science, CINAHL and Global Health for observational studies reporting ESBL-P<i>E</i> prevalence in cancer patients. Studies published in English from 1 January 2010 to 31 December 2024 were included. Two reviewers independently screened studies, extracted data using standardized forms and assessed the quality of articles using the Newcastle-Ottawa Scale. Pooled prevalence was calculated using a random-effects model in RStudio v4.4.2, with heterogeneity assessed with the <i>I</i>² statistic and publication bias assessed with funnel plots and Egger's test. <b>Results.</b> Twelve studies from 9 African countries, involving a total of 1,252 cancer patients and 643 identified events, were included. The pooled prevalence of ESBL-P<i>E</i> was 49.4% (95% CI: 36.0-62.9%, <i>I</i>²=88.3%, <i>P</i><0.001). <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> predominated. There was no evidence of publication bias based on funnel plot symmetry, Egger's test (<i>t</i>=-0.35, <i>P</i>=0.73) and trim-and-fill analysis (no studies imputed; adjusted estimate unchanged). Heterogeneity was substantial (<i>I</i>²=88.3%) with a wide 95% prediction interval (22.7-76.5%). Leave-one-out sensitivity analysis confirmed estimate stability (~49%) with persistently high heterogeneity (<i>I</i>² range: 81.4-89.4%). <b>Conclusions.</b> The high ESBL-P<i>E</i> prevalence in African cancer patients signals a critical public health issue, necessitating enhanced surveillance, antimicrobial stewardship and intentional infection control measures.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"8 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13262598/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148255371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplate-based quantification of poly-γ-glutamic acid levels in biofilm samples.","authors":"David Stevenson, Cait E MacPhee, Nicola Stanley-Wall","doi":"10.1099/acmi.0.001162.v4","DOIUrl":"10.1099/acmi.0.001162.v4","url":null,"abstract":"<p><p>Poly-<i>γ</i>-glutamic acid (PGA) is a commercially useful biopolymer produced by many <i>Bacillus</i> species. PGA has a diverse range of applications across medicine and industry, generating significant interest in optimizing PGA production and enhancing yields. One approach to improve PGA recovery involves identifying high-yield PGA-producing strains and determining optimal production conditions, both of which require an appropriate screening method. Here, we present a sensitive and reproducible assay for quantifying PGA from <i>Bacillus subtilis</i> biofilms, whereby the spectral profile of methylene blue changes when bound to PGA. PGA was purified and lyophilized from NCIB 3610 Δ<i>tasA</i> liquid cultures grown at 50 °C, allowing production of protein-free PGA for use as a standard at known concentrations. Standard curves were generated from methylene blue absorbance readings at 564 and 664 nm, enabling subsequent quantification of PGA from biofilm extracts. We validated the quantification protocol and determined the treatment steps required to minimize interference. The assay has a 96-well plate format, enabling quantification of many samples at low sample volume, while minimizing waste of laboratory consumables. Overall, our method offers a sensitive, reproducible approach for PGA quantification in biofilm research and should facilitate comparative analyses across strains, treatments or environmental conditions.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"8 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13249132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148221432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity and plant growth-promoting traits of endophytic bacteria isolated from maize cropped in organic and low-input agricultural systems in southern Brazil.","authors":"Dafila Santos Lima Fagotti, Josiane Fukami, Paula Cerezini, Andre Luiz Martinez Oliveira, Galdino Andrade, Milena Serenato Klepa, Luisa Carolina Ferraz Helene, Ligia Maria Oliveira Chueire, Renan Augusto Ribeiro, Mariangela Hungria, Marco Antonio Nogueira","doi":"10.1099/acmi.0.000948.v3","DOIUrl":"10.1099/acmi.0.000948.v3","url":null,"abstract":"<p><p>Plant growth-promoting bacteria (PGPB) may enhance plant growth and health through several mechanisms, contributing to sustainable agriculture. We isolated and characterized endophytic bacteria from stems of landrace maize (<i>Zea mays</i> L.) grown under low-input and organic systems in southern Brazil. The 16S rRNA analysis revealed 83 isolates within 14 genera, including <i>α</i>-Proteobacteria, <i>β-Proteobacteria</i>, <i>γ-Proteobacteria</i>, <i>Actinomycetia</i> and <i>Bacilli</i> classes. The synthesis of indolic compounds was widespread among the strains, as well as the enhancement of N concentration in N-free media, a putative trait for the capacity of biological nitrogen fixation. Phosphate solubilization prevailed in <i>Burkholderia</i> and <i>Paraburkholderia</i> strains, while siderophore production was observed in all genera except <i>Microbacterium</i>. Inoculation with selected strains confirmed growth promotion on maize plantlets, particularly <i>Sphingomonas</i> CNPSo 2378 and <i>Bacillus velezensis</i> CNPSo 2384, whose genomes were sequenced and revealed PGPB features and evolutionary relationships, shedding light on the diversity, functional traits and occurrence of PGPB in low-input and organic agricultural systems.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"8 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13232961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148166404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Management of infective endocarditis in a secondary care trust: a service evaluation of treatment outcomes and multidisciplinary involvement.","authors":"Lisa Dwyer-Joyce, Mo Chung Kwok, Stephen Hughes, Nupur Goel","doi":"10.1099/acmi.0.001153.v3","DOIUrl":"10.1099/acmi.0.001153.v3","url":null,"abstract":"<p><p><b>Background.</b> Infective endocarditis (IE) is a complex and life-threatening condition, requiring multidisciplinary team (MDT) input for optimal management. <b>Objectives.</b> This study aimed to review the demographics, infection management and clinical outcomes of patients treated for IE at Chelsea and Westminster Hospital NHS Foundation Trust (CWFT), a secondary care provider in London. This study evaluated local IE service provision against national and international guidelines to identify areas for service improvement. <b>Methods.</b> A retrospective cohort study was conducted, identifying adult patients (≥18 years) treated for IE at CWFT between September 2023 and August 2024. Patients who received >10 days of intravenous amoxicillin, flucloxacillin, cefazolin or vancomycin were screened. Patients who received a full course of IE treatment were included. Clinical records were reviewed for demographic, microbiological, imaging, MDT involvement and clinical outcome data. <b>Results.</b> Thirty-one patients met the inclusion criteria. The median age was 77 years (interquartile range 53-85), and 74% were male. <i>Staphylococcus aureus</i> (35%) and <i>Enterococcus faecalis</i> (29%) were the most common pathogens. Only 12 patients (39%) were discussed at a cardiothoracic MDT, and 2 patients lacked cardiology input. Microbiology consultations occurred in all cases, but only 55% had documented antimicrobial plans. Outpatient therapy was used in 36% of cases. Adverse outcomes of IE treatment included in-hospital mortality in 12.9% (<i>n</i>=4) and decline in functional baseline in 29% (<i>n</i>=9). <b>Conclusions.</b> Whilst key treatment standards were largely met, gaps in MDT involvement and documentation were identified. Implementation of a dedicated IE MDT may increase adherence to guidelines and improve secondary care service provision.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"8 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13232865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148166360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative phenotypic and genomic analysis of the methanogen Methanomethylovorans thermophila L2FAW and its phylogenomic placement within the Genome Taxonomy Database.","authors":"Mathias Wunderer, Andja Mullaymeri, Andreas O Wagner, Eva Maria Prem","doi":"10.1099/acmi.0.001123.v4","DOIUrl":"10.1099/acmi.0.001123.v4","url":null,"abstract":"<p><p>The genome of the methylotrophic methanogen <i>Methanomethylovorans thermophila</i> L2FAW is not included in the Genome Taxonomy Database (GTDB) so far, even though the strain was first described in 2005. To evaluate its genomic characteristics and placement in the GTDB, we sequenced the genome of <i>M. thermophila</i> L2FAW via <i>Illumina</i> shotgun and Oxford <i>Nanopore</i> sequencing and subsequently did hybrid assembly. The assembled genome consists of 2.25 Mbp (contigs ≥500 bp) with a G+C content of 40 mol%. The quality of the genome is good, which is already apparent from the low L50 (=1) and L90 (=2) metrics. Our assembled genome was highly similar to the metagenome-assembled genome <i>Methanomethylovorans sp014361205</i> (GCA_014361205.1_ASM1436120v1_genomic) with an average nucleotide identity of 99.9%. Even though KEGG Mapper Reconstruction results revealed that <i>M. thermophila</i> L2FAW harbours all the enzymes necessary for acetoclastic and hydrogenotrophic methanogenesis and <i>gapseq</i> predicted formate as a potential substrate for <i>M. thermophila</i> L2FAW, no metabolic activity could be observed on acetate, H₂-CO₂ (80:20 vol/vol, 2,000 mbar) and on a mixture of H₂-CO₂ and formate in lab tests; thus, the obligate methylotrophic lifestyle of the phenotype was confirmed.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"8 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13193254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148019813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}