Access microbiology最新文献_第7页

Erratum: Towards an inclusive conference experience: evaluation of the Education and Outreach Symposium at the Microbiology Society Annual Conference 2024. 勘误：迈向一个包容性的会议经验：在微生物学会年度会议2024年教育和推广研讨会的评估。

Access microbiology Pub Date : 2025-05-08 eCollection Date: 2025-01-01 DOI: 10.1099/acmi.0.001048

Melissa M Lacey, Michael J Dillon, Sean Goodman, Victoria Easton, Alison I Graham

引用次数: 0

Gummatous mitral valve endocarditis from tertiary syphilis. 三期梅毒所致的牙龈性二尖瓣心内膜炎。

Access microbiology Pub Date : 2025-05-08 eCollection Date: 2025-01-01 DOI: 10.1099/acmi.0.000817.v3

Nisha George, Daniel Pan, Shirley Sze, Caroline Williams, Zein El-Dean, Victor Zlocha, Elizabeth Webb, Manish Pareek

{"title":"Gummatous mitral valve endocarditis from tertiary syphilis.","authors":"Nisha George, Daniel Pan, Shirley Sze, Caroline Williams, Zein El-Dean, Victor Zlocha, Elizabeth Webb, Manish Pareek","doi":"10.1099/acmi.0.000817.v3","DOIUrl":"10.1099/acmi.0.000817.v3","url":null,"abstract":"A 50-year-old Romanian gentleman presented with fever, myalgia and 30 kg weight loss. He was treated for syphilis after acquiring it 16 years ago. On examination, there was a pansystolic murmur in the axilla, and the patient had an ataxic gait. Blood tests showed raised inflammatory markers. However, standard investigations for infective endocarditis, including multiple blood cultures, serological titres for fastidious organisms and antibody tests were negative. A computed tomography (CT) of the chest, abdomen and pelvis demonstrated hepatosplenomegaly with multiple splenic infarcts. A magnetic resonance imaging (MRI) of the head with contrast showed multiple punctate enhancement in the bilateral hemispheres with leptomeningeal enhancement. Transthoracic echocardiogram demonstrated a large vegetation leading to severe mitral regurgitation. Serum treponemal antibodies were positive; Treponema pallidum particle agglutination (TPPA) was positive at 1 : 1280, and rapid plasma reagin (RPR) 1 : 4 treponemal IgM was negative; lumbar puncture syphilis serology was negative. The patient was treated with an extensive period of intravenous antibiotics, in addition to a prosthetic metallic valve replacement, where unusual ragged calcified valvular tissue was observed. Tertiary syphilis is a difficult diagnosis to confirm, since it can often be indolent and occur in areas of the body where it may go unnoticed. In our case, a diagnosis of probable syphilitic endocarditis was made from a combination of the history, an initial increase in the size of the lesion following antibiotic therapy and observation of likely gumma on the mitral valve during surgery. In such cases, surgery in addition to optimal antimicrobial therapy is necessary for effective treatment. This case adds to the current literature that treatment with penicillin is likely inadequate to prevent late complications.","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144692973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Primary iliac bone tuberculosis: a case report. 原发性髂骨结核1例。

Access microbiology Pub Date : 2025-05-07 eCollection Date: 2025-01-01 DOI: 10.1099/acmi.0.000844.v3

Hajar Dahou, Fataou Saley Younoussa, Imane Aragon, Salma El Aouadi, Yahya El Harras, Elmostafa Benaissa, Yassine Ben Lahlou, Abdelaali Bahadi, Jamal El Fenni, Adil Maleb, Mariama Chadli, Mostafa Elouennass

引用次数: 0

Corrigendum: Tossing the coin of extended-spectrum β-lactamase: prevalence of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolated from patients with sepsis. 勘误：抛掷广谱β-内酰胺酶的硬币：从脓毒症患者中分离出的广谱β-内酰胺酶产生肺炎克雷伯菌的患病率。

Access microbiology Pub Date : 2025-05-07 eCollection Date: 2025-01-01 DOI: 10.1099/acmi.0.001047

Beatrice Achan, Tonny Luggya, Robert Innocent Ebwongu, Simon Sekyanzi, Henry Kajumbula

引用次数: 0

Cardiac tamponade revealing tuberculous pericarditis: a case report. 心包填塞显示结核性心包炎1例。

Access microbiology Pub Date : 2025-05-02 eCollection Date: 2025-01-01 DOI: 10.1099/acmi.0.000983.v4

Oumaima Skalante, Mariam Hachimi Idrissi, Soukaina Cherkaoui, Elmostafa Benaissa, Yassine Ben Lahlou, Mariama Chadli

{"title":"Cardiac tamponade revealing tuberculous pericarditis: a case report.","authors":"Oumaima Skalante, Mariam Hachimi Idrissi, Soukaina Cherkaoui, Elmostafa Benaissa, Yassine Ben Lahlou, Mariama Chadli","doi":"10.1099/acmi.0.000983.v4","DOIUrl":"https://doi.org/10.1099/acmi.0.000983.v4","url":null,"abstract":"Tuberculosis remains a major public health issue in Morocco. Pulmonary tuberculosis is the most common form, but various extrapulmonary forms exist. Tuberculous pericarditis is a rare form of extrapulmonary tuberculosis that can be complicated by cardiac tamponade, pericardial constriction or their combination, which can threaten the patient's life. Its clinical and radiological signs are nonspecific, and the clinical presentation can be misleading and incomplete, sometimes even with an initial tamponade. We report the case of a 68-year-old female patient admitted for intense retrosternal chest pain associated with acute dyspnoea, evolving in the context of unquantified weight loss and general deterioration. Additionally, she reported a history of fever and night sweats. Clinical examination revealed a conscious, febrile, hypotensive, tachycardic, polypneic patient with good oxygen saturation, signs of right heart failure and muffled heart sounds on auscultation. Chest X-ray revealed cardiomegaly, and the ECG showed diffuse low voltage. Given the presence of Beck's triad suggestive of cardiac tamponade, a transthoracic echocardiogram was performed, revealing a large pericardial effusion with a 'swinging heart'. A chest CT scan also confirmed the large pericardial effusion. The diagnosis of cardiac tamponade was made based on the clinical and radiological findings, and pericardial drainage was performed, after which the patient showed clinical improvement. PCR GenXpert MTB/RIF Ultra detected the presence of Mycobacterium tuberculosis in the pericardial fluid, with no resistance to rifampicin. Culture was positive for M. tuberculosis. The diagnosis of tuberculous pericarditis was, thus, confirmed, and the patient was started on quadruple antituberculosis therapy with good clinical progress.","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048714/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144065545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel hot spring Thermoproteota support vertical inheritance of ammonia oxidation and carbon fixation in Nitrososphaeria. 新型温泉温虫支持亚硝基球藻氨氧化和固碳的垂直遗传。

Access microbiology Pub Date : 2025-04-29 eCollection Date: 2025-01-01 DOI: 10.1099/acmi.0.000931.v4

T Slosser, M Wenick, E Markert, E Trembath-Reichert, L M Ward

{"title":"Novel hot spring Thermoproteota support vertical inheritance of ammonia oxidation and carbon fixation in Nitrososphaeria.","authors":"T Slosser, M Wenick, E Markert, E Trembath-Reichert, L M Ward","doi":"10.1099/acmi.0.000931.v4","DOIUrl":"https://doi.org/10.1099/acmi.0.000931.v4","url":null,"abstract":"Aerobic ammonia oxidation is crucial to the nitrogen cycle and is only known to be performed by a small number of bacterial lineages [ammonia-oxidizing bacteria (AOB)] and a single lineage of archaea belonging to the Nitrososphaeria class of Thermoproteota [ammonia-oxidizing Archaea (AOA)]. Most cultivated AOA originate from marine or soil environments, but this may capture only a limited subset of the full diversity of this clade. Here, we describe several genomes of AOA from metagenomic sequencing of a hot spring microbial mat, representing several poorly characterized basal lineages that may be important for understanding the early evolution of archaeal ammonia oxidation. These genomes include a novel genus most closely related to Nitrososphaera as well as novel species belonging to the genera Nitrosotenuis, Nitrososphaera and Nitrosotalea. Furthermore, the distributions and phylogenetic relationships of key metabolic genes support a history of vertical inheritance of ammonia oxidation and carbon fixation from the last common ancestor of crown group AOA.","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144045296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Faecal metagenomes of great tits and blue tits provide insights into host, diet, pathogens and microbial biodiversity. 大山雀和蓝山雀的粪便宏基因组提供了对宿主、饮食、病原体和微生物多样性的深入了解。

Access microbiology Pub Date : 2025-04-28 eCollection Date: 2025-01-01 DOI: 10.1099/acmi.0.000910.v3

Mark J Pallen, Alise Jany Ponsero, Andrea Telatin, Cara-Jane Moss, David Baker, Darren Heavens, Gabrielle L Davidson

{"title":"Faecal metagenomes of great tits and blue tits provide insights into host, diet, pathogens and microbial biodiversity.","authors":"Mark J Pallen, Alise Jany Ponsero, Andrea Telatin, Cara-Jane Moss, David Baker, Darren Heavens, Gabrielle L Davidson","doi":"10.1099/acmi.0.000910.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000910.v3","url":null,"abstract":"Background. The vertebrate gut microbiome plays crucial roles in host health and disease. However, there is limited information on the microbiomes of wild birds, most of which is restricted to barcode sequences. We therefore explored the use of shotgun metagenomics on the faecal microbiomes of two wild bird species widely used as model organisms in ecological studies: the great tit (Parus major) and the Eurasian blue tit (Cyanistes caeruleus). Results. Short-read sequencing of five faecal samples generated a metagenomic dataset, revealing substantial variation in composition between samples. Reference-based profiling with Kraken2 identified key differences in the ratios of reads assigned to host, diet and microbes. Some samples showed high abundance of potential pathogens, including siadenoviruses, coccidian parasites and the antimicrobial-resistant bacterial species Serratia fonticola. From metagenome assemblies, we obtained complete mitochondrial genomes from the host species and from Isospora spp., while metagenome-assembled genomes documented new prokaryotic species. Conclusions. Here, we have shown the utility of shotgun metagenomics in uncovering microbial diversity beyond what is possible with 16S rRNA gene sequencing. These findings provide a foundation for future hypothesis testing and microbiome manipulation to improve fitness in wild bird populations. The study also highlights the potential role of wild birds in the dissemination of antimicrobial resistance.","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12038002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144053766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial activity of Cinnamomum verum and Thymus vulgaris essential oils on multidrug-resistant zoonotic bacteria isolated from dogs in southern Benin. verum verum和thyymus vulgaris精油对贝宁南部犬源多重耐药人畜共患细菌的抑菌活性。

Access microbiology Pub Date : 2025-04-28 eCollection Date: 2025-01-01 DOI: 10.1099/acmi.0.000975.v3

Ayaovi Bruno Yaovi, Arpita Das, Rama N Behera, Paulin Azokpota, Souaïbou Farougou, Lamine Baba-Moussa, Franck Michels, Marie-Laure Fauconnier, Kiran Ambatipudi, Philippe Sessou

{"title":"Antibacterial activity of Cinnamomum verum and Thymus vulgaris essential oils on multidrug-resistant zoonotic bacteria isolated from dogs in southern Benin.","authors":"Ayaovi Bruno Yaovi, Arpita Das, Rama N Behera, Paulin Azokpota, Souaïbou Farougou, Lamine Baba-Moussa, Franck Michels, Marie-Laure Fauconnier, Kiran Ambatipudi, Philippe Sessou","doi":"10.1099/acmi.0.000975.v3","DOIUrl":"10.1099/acmi.0.000975.v3","url":null,"abstract":"Antibiotic resistance is a major public health problem. The search for new therapeutic alternatives is becoming urgent. Essential oils are a promising alternative. This study aimed to evaluate the antibacterial activities of essential oils from selected plants on multidrug-resistant zoonotic strains isolated from dogs. Essential oils from dried Thymus vulgaris leaves, Cinnamomum verum bark and Cuminum cyminum seeds were extracted and tested on five multidrug-resistant Escherichia coli and four Staphylococcus aureus isolated from dogs in southern Benin. The study showed that T. vulgaris essential oil was bacteriostatic, with an MIC equal to 2.5 µl ml-1 and a minimum bactericidal concentration (MBC) of 17 µl ml-1 for E. coli strains and 11.25 µl ml-1 for S. aureus strains. Regarding C. verum essential oil, its bacteriostatic power was characterized by an MIC of 1.25 µl ml-1 for the isolates tested and an average MBC of 11.50 µl ml-1 for E. coli and 12.19 µl ml-1 for S. aureus. On the other hand, C. cyminum essential oil was ineffective on the strains investigated. Additionally, T. vulgaris essential oil contained predominantly thymol (36.57%), p-cymene (30.51%) and carvacrol (7.62%), whilst C. verum essential oil contained cinnamaldehyde (88.76%). This study reveals the antibacterial activity of T. vulgaris dry leaf and C. verum bark essential oils on multi-resistant E. coli and S. aureus isolated from dogs. These two essential oils may be alternative candidates for combating antibiotic-resistant E. coli and S. aureus infections.","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12451634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome sequence of antibiotic-resistant Klebsiella quasipneumoniae FSFC0558: a novel sequence type (ST8212). 耐药准肺炎克雷伯菌FSFC0558基因组序列：一个新的序列类型（ST8212）。

Access microbiology Pub Date : 2025-04-25 eCollection Date: 2025-01-01 DOI: 10.1099/acmi.0.000944.v3

Daniel Robins, Richard N Goodman, Ralfh Pulmones, Upendo O Kibwana, Joel Manyahi, Bjørn Blomberg, Nina Langeland, Sabrina Moyo, Adam P Roberts

引用次数: 0

Opportunities for microbiology citizen science: lessons learnt from three pilot projects. 微生物学公民科学的机会：三个试点项目的经验教训。

Access microbiology Pub Date : 2025-04-16 eCollection Date: 2025-01-01 DOI: 10.1099/acmi.0.000899.v3

Rachel M Pateman, Joyce Bennett, Anthony C Hilton, Isabella Romeo-Melody, Anton Rosenfeld, Sarah J Routledge, Caroline Rymer, Benjamin M C Swift, Lucy Way, Louise Whatford, Naomi C Wilkinson, Tony Worthington, Lewis Yandle, Ayesha S Younis, Sarah E West, Alan D Goddard

{"title":"Opportunities for microbiology citizen science: lessons learnt from three pilot projects.","authors":"Rachel M Pateman, Joyce Bennett, Anthony C Hilton, Isabella Romeo-Melody, Anton Rosenfeld, Sarah J Routledge, Caroline Rymer, Benjamin M C Swift, Lucy Way, Louise Whatford, Naomi C Wilkinson, Tony Worthington, Lewis Yandle, Ayesha S Younis, Sarah E West, Alan D Goddard","doi":"10.1099/acmi.0.000899.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000899.v3","url":null,"abstract":"Citizen science (CS) is the partnering of professional scientists and members of the public to answer real-world scientific questions. There has been huge growth in CS over the past two decades, but uptake in microbiology research has, thus far, been relatively limited. In the first part of this article, we discuss how CS is well aligned with microbiology research: sample collection methods can be simplified and used in a variety of environments; projects are expected to appeal to participants as topics are likely to be of relevance to people's lives and interests, including the health of people and the environment; and projects can also lead to real-world impact, including the identification of new drugs or biotechnological solutions. In the second part of this article, we present our reflections on three pilot projects we have recently completed. In order for the field to grow, people need to share both their successes as well as the challenges they have faced, so that others wanting to use the method can learn from these experiences. We share simplified sampling methods for yeast strains from home brewing and baking, antimicrobial-resistant bacteria on home-grown produce and microbes on chopping boards. However, participation in our projects was limited by a range of factors, including time available and resourcing, which impacted on our ability to generate new knowledge and wider impacts. We provide recommendations for others wishing to run microbiology CS projects, including ensuring appropriate resourcing and considering the ethical implications of projects.","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12003925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144065543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0