Access microbiology最新文献

{"title":"Epidemiological and virological characteristics of people living with HIV on antiretroviral treatment for more than 6 months in virological failure in Brazzaville, Republic of Congo.","authors":"Ferdinand Emaniel Brel Got, Gervillien Arnold Malonga, Juthèce Privat Malanda-Kiminou, Maryse Akolbout, Ghislain Loubano-Mvoumbi, Dagene Ebourombi, Merlin Diafouka, Gontran Ondzotto","doi":"10.1099/acmi.0.000805.v3","DOIUrl":"10.1099/acmi.0.000805.v3","url":null,"abstract":"<p><p><b>Introduction.</b> Virological failure is one of the main causes of failing to treat, and better management of HIV infection requires understanding and controlling the factors that contribute to this phenomenon. The main objective was to characterize the patients of the active file of the Brazzaville Outpatient Treatment Center in virological failure to identify predictive factors leading to virological failure. <b>Methods.</b> Conducted between June and December 2020, this was a cross-sectional study. Patients enrolled were HIV-1-infected patients from the Brazzaville Outpatient Treatment Center receiving a potent combination therapy for at least 6 months but experiencing virological failure. Viral load was measured using the automated Abbott Real-time HIV-1 m2000rt System. Sociodemographic and clinical data were collected from a computerized patient record software called Santia. For the identification of the independent predictors of virological failure, statistical analysis was performed. <b>Results.</b> A total of 109 patients with virological failure were recruited. The median age of the patients was 45 years (interquartile range: 37-52 years) and women were more represented (74%). More than half of the patients had World Health Organization stage IV HIV and the median duration of antiretroviral treatment was 96 months. The most followed treatment regimen was AZT+3TC+EFV (or nevirapine) with 48%, while the median viral load was 12985 copies ml^-1. <b>Conclusion.</b> In our study, we did not identify any sociodemographic or clinical variables predictive of virological failure. However, we felt that it would be desirable to carry out a study with temporal follow-up and the possibility of sequencing in order to identify the different circulating genotypes and resistance mutations.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11652753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome sequence data for 61 isolates of Xanthomonas campestris pv. campestris from Brassica crops in Serbia.","authors":"Tatjana Popović Milovanović, Shannon Greer, Renata Iličić, Aleksandra Jelušić, Daisy Bown, Mikaeel Hussain, Jamie Harrison, Murray Grant, Joana G Vicente, David J Studholme","doi":"10.1099/acmi.0.000870.v3","DOIUrl":"10.1099/acmi.0.000870.v3","url":null,"abstract":"<p><p>This Technical Resource describes genome sequencing data for 61 isolates of the bacterial pathogen <i>Xanthomonas campestris</i> pv. <i>campestris</i> collected from <i>Brassica</i> and <i>Raphanus</i> crops between 2010 and 2021 in Serbia. We present the raw sequencing reads and annotated contig-level genome assemblies and determine the races of ten isolates. The data can be used to test hypotheses and phylogeographic analyses and inform the design of informative molecular markers for population genetics studies. When combined with phenotypic data, they could be used to dissect relationships between genotypes and phenotypes such as host range and virulence. Finally, these genome sequences expand our inventory of plasmids known to reside in this pathogen.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity of HBV genotypes and their association with precore/basal core mutations among HBsAg-positive patients in Ibadan, Nigeria.","authors":"Adedayo Omotayo Faneye, Babatunde Olanrewaju Motayo, Aisha Mustafa, Georgina Odiabo","doi":"10.1099/acmi.0.000821.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000821.v3","url":null,"abstract":"<p><p><b>Background.</b> Hepatitis B virus (HBV) is the most implicated cause of severe liver disease and hepatocellular carcinoma worldwide. Studies have shown that the basal core protein (BCP) and precore protein (PC) of HBV play a significant role in HBV-related carcinogenesis. There is a paucity of data on the type and effect of BCP and PC mutations in Nigeria. This study aims to genotype HBV and investigate any mutations within the BCP and PC among HBV patients in Ibadan, Nigeria. <b>Methods.</b> Forty HBV-DNA-positive patients were recruited into this study, and the viral load assay and genotyping by nested multiplex PCR were done. The partial X gene region was amplified and Sanger sequenced. The BPC and PC genomic regions were then analysed using bioinformatics. <b>Results.</b> Twenty-three participants recorded HBV DNA viral load of >20 000 IU, while 17 had <20 000 IU and 28 samples were genotyped. Five genotypes (A, B, C, D and E) and four mixed genotypes (AC, AD ACD and ABCD) were detected. Genotype AC was the most frequently encountered, while genotypes E and B were the least encountered. Mutation was highest in ages 34-45 years. Double mutation A1762T and G1764A within the BCP region was the most encountered mutation. <b>Conclusions.</b> We report a diverse HBV genetic landscape, with mixed infections between genotypes with BCP double-mutation A1762T/G1764A, signalling the likelihood of poor HBV-related liver disease prognosis. Our findings contribute to our understanding of the molecular characteristics of HBV and its potential implications for disease progression and management among HBV-infected Nigerians.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11542583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erysipelothrix rhusiopathiae-associated bloodstream infection in a patient with systemic lupus erythematosus: a case report and literature review.","authors":"Calvin Ka-Fung Lo, Cole Schonhofer, Neil Mina, Shazia Masud, Patrick Ho Pun Wong, Michael G Chapman","doi":"10.1099/acmi.0.000881.v3","DOIUrl":"10.1099/acmi.0.000881.v3","url":null,"abstract":"<p><p><b>Introduction.</b> Systemic human infections caused by <i>Erysipelothrix rhusiopathiae</i> have been increasingly reported especially within immunocompromised hosts and those with significant occupational exposure to livestock and aquatic animals. We report a case of <i>E. rhusiopathiae</i> bacteraemia in a patient with systemic lupus erythematosus (SLE) and present a literature review on clinical outcomes and microbiologic diagnosis for this organism. <b>Casepresentation.</b> A 43-year-old female patient was reporting a 1-month history of intermittent fevers. She recently increased her immunosuppression medication for her underlying SLE on the advice of her rheumatologist. The patient sustained a finger laceration from butchering cattle meat 2 weeks after the onset of her initial symptoms, with worsening index finger swelling and increased febrile episodes. Two weeks post-injury, multiple blood cultures were drawn, and each isolated Gram-positive bacilli. Given her recurrent intermittent fevers, there was a concern for ongoing infection, and therefore, intravenous vancomycin was started with prompt referral to an outpatient parenteral antibiotic therapy clinic. The Gram-positive bacillus was confirmed as <i>E. rhusiopathiae</i> via matrix-assisted laser desorption/ionization-time of flight analysis. Given intrinsic resistance to vancomycin, vancomycin was switched to intravenous ceftriaxone as targeted antimicrobial therapy for 2 weeks. Reassuringly, there was no echocardiographic evidence of infective endocarditis, warranting the prolonged treatment course. Post-treatment, she remained symptom-free with the resolution of joint symptoms and fevers. <b>Conclusion.</b> Our report illustrates a case of <i>E. rhusiopathiae</i> bacteraemia from an immunodeficient host, with prompt microbiologic diagnosis and intervention with appropriate antimicrobial coverage. Literature reflects the rarity of this infection, predilections to specific susceptible hosts and the importance of raising awareness of zoonotic infections.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11649197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome sequencing and analysis of Salmonella enterica subsp. enterica serotype Enteritidis PT4 578: insights into pathogenicity and virulence.","authors":"Deisy G Carneiro, Pedro Marcus P Vidigal, Túlio Morgan, Maria Cristina D Vanetti","doi":"10.1099/acmi.0.000828.v3","DOIUrl":"10.1099/acmi.0.000828.v3","url":null,"abstract":"<p><p><i>Salmonella enterica</i> serotype Enteritidis is a generalist serotype that adapts to different hosts and transmission niches. It has significant epidemiological relevance and is among the most prevalent serotypes distributed in several countries. <i>Salmonella</i> Enteritidis causes self-limited gastroenteritis in humans, which can progress to systemic infection in immunocompromised individuals. The <i>Salmonella</i> pathogenicity mechanism is multifactorial and complex, including the presence of virulence factors that are encoded by virulence genes. Poultry products are considered significant reservoirs of many <i>Salmonella</i> serotypes, and <i>Salmonella</i> Enteritidis infections are often related to the consumption of chicken meat and eggs. This study reports the whole-genome sequence of <i>Salmonella</i> Enteritidis PT4 strain 578. A total of 165 genes (3.66%) of the 4506 coding sequences (CDS) predicted in its genome are virulence factors associated with cell invasion, intestinal colonization, and intracellular survival. The genome harbours twelve <i>Salmonella</i> pathogenicity islands (SPIs), with the SPI-1 and SPI-2 genes encoding type III secretion systems (T3SS) showing high conservation. Six prophage-related sequences were found, with regions of intact prophages corresponding to <i>Salmon_118970_sal3</i> and <i>Gifsy-2</i>. The genome also contains two CRISPR systems. Comparative genome analysis with <i>Salmonella</i> Enteritidis ATCC 13076, <i>Salmonella</i> Typhimurium ATCC 13311, and <i>Salmonella</i> Typhimurium ATCC 14028 demonstrates that most unshared genes are related to metabolism, membrane, and hypothetical proteins. Finally, the phenotypic characterization evidenced differences among <i>Salmonella</i> Enteritidis PT4 578 and the other three serotypes regarding the expression of the red, dry, and rough (rdar) morphotype and biofilm formation. Overall, the genomic characterization and phenotypic properties expand knowledge of the mechanisms of pathogenicity in <i>Salmonella</i> Enteritidis PT4 578.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11649194/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salmonella Weltevreden lung abscess and empyema without preceding gastrointestinal symptoms: an emerging pathogen in Australia?","authors":"Victoria Grey, Ernest Tee, Lauren Phillips, Gino Micalizzi, Mark Armstrong","doi":"10.1099/acmi.0.000635.v3","DOIUrl":"10.1099/acmi.0.000635.v3","url":null,"abstract":"<p><p>Non-typhoidal <i>Salmonella</i> lung infections are rare and are usually confined to immunocompromised hosts. Previous case reports have found that usually patients have either gastroenteritis or bacteraemia in addition to pulmonary involvement. We present the first known reported case of a <i>Salmonella</i> Weltevreden lung abscess and empyema in an immunocompetent patient without gastroenteritis. Despite the use of antimicrobials active against the pathogen, the patient needed surgical intervention to achieve adequate source control. While <i>S</i>. Weltevreden has previously been associated with returned travellers, especially from Southeast Asia, its incidence in Queensland is now increasing. Therefore, it is important for clinicians to be aware of its potential severity as well as the range of presentations.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corynebacterium amycolatum peritonitis in a patient undergoing peritoneal dialysis: case report and literature review.","authors":"Fatima Zahra Adil, Imane Aragon, Elmostafa Benaissa, Yassine Ben Lahlou, Fatna Bssaibis, Adil Maleb, Mariama Chadli, Mostafa Elouennass","doi":"10.1099/acmi.0.000880.v3","DOIUrl":"10.1099/acmi.0.000880.v3","url":null,"abstract":"<p><p>Peritoneal dialysis is a blood purification technique used in cases of end-stage chronic kidney failure, based on the filtering capabilities of the peritoneum. Infections, often caused by poor asepsis during catheter manipulation, are generally attributed to <i>Staphylococcus epidermidis</i> and <i>Staphylococcus aureus. Corynebacterium</i>, usually considered non-pathogenic, is rarely involved in these infections. We present a case of peritonitis due to <i>Corynebacterium amycolatum</i> in a patient undergoing peritoneal dialysis. The diagnosis was made based on cytobacteriological examination of the dialysate fluid, which on two occasions showed high levels of white blood cells with a predominance of neutrophilic polymorphonuclear and a monomorphic appearance of colonies on agar medium, whose identification by biochemical tests and antibiotic sensitivity study confirmed the presence of <i>C. amycolatum</i>. The patient was successfully treated with vancomycin, resulting in symptom resolution and sterilization of the dialysate fluid. Although rare, the involvement of <i>Corynebacterium</i> species underscores the importance of confirming its pathogenicity. Further studies are needed to better understand the epidemiology of these infections and guide future treatments. This case also highlights the need for a rigorous approach to confirming the pathogenicity of <i>Corynebacterium</i> despite its traditional classification as a contaminant.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bloodstream infections in cancer patients in central India: pathogens and trends of antimicrobial resistance over a 5-year period.","authors":"Sonali Choudhari, Ruchita Gawande, Jerestin Watchmaker, Pooja Bamnote, Pradeep Mishra, Pankaj Dwivedi","doi":"10.1099/acmi.0.000673.v5","DOIUrl":"https://doi.org/10.1099/acmi.0.000673.v5","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Introduction.&lt;/b&gt; Bloodstream infection (BSI) is a common complication with a high fatality rate in cancer patients. There are notable variations in the epidemiology of BSI over time and among different countries. Infections due to multidrug-resistant organisms (MDROs) such as extended-spectrum beta-lactamases (ESBLs) and carbapenem-resistant &lt;i&gt;Enterobacteriaceae&lt;/i&gt; (CRE) are increasing. This may lead to inadequate empirical antibiotic therapy, increasing the antimicrobial resistance (AMR) problem and unfavourable outcomes in these immunocompromised patients. There is paucity of data pertaining to AMR in such vulnerable patients from developing countries such as India. The aim of this study was to investigate the distribution of the bacterial pathogens causing BSI and the AMR trend in cancer patients in central India. &lt;b&gt;Methodology.&lt;/b&gt; This single-centre retrospective observational study was conducted in a tertiary care cancer hospital. Patients with solid organ and haematological malignancies, both adults and paediatric, who had blood cultures sent to the microbiology laboratory from January 2018 to December 2022 were included. Blood cultures were processed using the BacT/ALERT 3D system (bioMérieux, France), and the identification of the bacteria and their antimicrobial susceptibility (AST) was performed using the Vitek 2 compact system (bioMérieux, France). Electronic medical records and microbiology lab records were used to retrieve the demographic and microbiological data. Microsoft Excel (RRID:SCR_016137) was used to enter and tabulate the data. Statistical analysis was performed using SPSS version 29 (RRID:SCR_002865). &lt;b&gt;Results.&lt;/b&gt; A total of 687 isolates from 524 patients were studied. Gram-negative bacteria (64%) were the commonest cause of BSI in the studied patients, followed by Gram-positive cocci (25%) and fungal isolates (9%). Ten cases were polymicrobial. &lt;i&gt;Escherichia coli&lt;/i&gt; (&lt;i&gt;n&lt;/i&gt;=140) was the most common among the isolated pathogens, followed by &lt;i&gt;Klebsiella&lt;/i&gt; species (&lt;i&gt;n&lt;/i&gt;=103), &lt;i&gt;Pseudomonas&lt;/i&gt; species (&lt;i&gt;n&lt;/i&gt;=102), and coagulase-negative staphylococci (CONS) (&lt;i&gt;n&lt;/i&gt;=92). Among the 140 isolates of &lt;i&gt;E. coli&lt;/i&gt;, 66% were extended-spectrum β-lactamase (ESBL) producers and 26% were resistant to carbapenem. Among the 103 isolated &lt;i&gt;Klebsiella&lt;/i&gt; species, 50% were carbapenem resistant and 36% were ESBL producers. Among enterobacterales, the CRE rate was 34%. Carbapenem resistance was seen in 25% of &lt;i&gt;Pseudomonas&lt;/i&gt; species and 53% of &lt;i&gt;Acinetobacter&lt;/i&gt; species isolates. &lt;i&gt;Klebsiella&lt;/i&gt; species were the most resistant pathogens isolated. CONS comprised 56% of all Gram-positive isolates, followed by &lt;i&gt;Staphylococcus aureus&lt;/i&gt; (36%), enterococci species (11%), and streptococci species (3%). Methicillin resistance was 60% in CONS and 64% in &lt;i&gt;S. aureus&lt;/i&gt;. One vancomycin-resistant enterococcus was isolated. Non-&lt;i&gt;albicans Candida&lt;/i&gt; was the most common fungal pathogen. The sensitivity ","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meropenem-resistant Burkholderia pseudomallei: a concerning single case in Australia with no prior meropenem exposure.","authors":"Pirathaban Sivabalan, Ferris Satyaputra, Ian Gassiep, Brian Forde, Jaimie Frazer, Matthew Glover, Buenafe Adams, Robert Norton","doi":"10.1099/acmi.0.000619.v4","DOIUrl":"10.1099/acmi.0.000619.v4","url":null,"abstract":"<p><p>We report a case of cutaneous melioidosis in a 54-year-old male with a meropenem-resistant sub-population. He was empirically treated with episodic doxycycline and trimethoprim-sulfamethoxazole; however, the abscess re-accumulated. The patient had no prior exposure to meropenem. A sub-population of the isolate was meropenem resistant with an MIC >32 µg ml^-1 and the identification was re-confirmed as <i>Burkholderia pseudomallei</i>. Whole-genome sequencing with ARDaP analysis only revealed a resistance determinant to doxycycline and did not reveal a resistance determinant to meropenem. Furthermore, no carbapenemases were detected through multiple bioinformatics tools. To date, this is the first reported case in Australia of a <i>B. pseudomallei</i> isolate resistant to meropenem without previous carbapenem exposure.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11652719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriological profile of community peritonitis operated in a Moroccan Hospital.","authors":"Samia Bazhar, Elmostafa Benaissa, Fatima Ziad, Leila Laamara, Yassine Ben Lahlou, Mariama Chadli, Mostafa Elouennass","doi":"10.1099/acmi.0.000816.v5","DOIUrl":"https://doi.org/10.1099/acmi.0.000816.v5","url":null,"abstract":"<p><p><b>Introduction.</b> Peritonitis is characterized by acute inflammation of the peritoneum, often resulting from digestive organ perforation or intra-abdominal septic focus. It may be of either infectious or non-infectious origin. The bacteria involved are those of the digestive flora (<i>Enterobacteriaceae</i> and anaerobes), while Gram-positive cocci and yeasts can be isolated in nosocomial infections. Our study aims to isolate and identify the germs involved in community-acquired peritonitis in order to assess their susceptibility to the antibiotics available in our country. <b>Methods.</b> This is a retrospective study of the bacteriological profile of community peritonitis in Rabat Morocco. A total of 150 adult patients with peritonitis were admitted and samples were collected intraoperatively for bacteriological examination between 1 July 2022 and 30 April 2023. <b>Results.</b> Among the 150 patients, 101 (67.8%) were males and 49 (32.2%) were females, with a sex ratio (M/F) of 2 : 1. The mean age of the patients was 40.5 years±20.12. The distribution of bacteria was dominated by <i>Escherichia coli</i> (44%). Overall, 70% of <i>E. coli</i> isolated exhibited resistance to ampicillin, whereas no resistance to ampicillin has been reported for <i>Enterococcus</i>. <b>Discussion.</b> In the present study, we were interested in the bacteriological profile of community peritonitis in order to adapt the antibiotic therapy to our bacterial ecology. Our findings indicate a concerning trend of increasing resistance among <i>E. coli</i> to the commonly used amoxicillin/clavulanic acid combination in our clinical setting. <b>Conclusion.</b> Consequently, there is a need to reassess the empiric antibiotic prescribed for the management of community-acquired peritonitis.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}