Access microbiology最新文献

{"title":"Whole genome sequencing assisted outbreak investigation of Salmonella enteritidis, at a hospital in South Africa, September 2022.","authors":"Brian Brümmer, Anthony Marius Smith, Motshabi Modise, Juno Thomas, Hetani Mdose, Ramasedi Mokoena, Dikeledi Baleni","doi":"10.1099/acmi.0.000835.v3","DOIUrl":"10.1099/acmi.0.000835.v3","url":null,"abstract":"<p><p>Health authorities were notified of a suspected outbreak of foodborne disease in a hospital in South Africa, where staff and patients reported acute onset of abdominal cramps, diarrhoea, fever and rigours after eating a chicken pasta meal. The aim of this report is to discuss the use of whole genome sequencing (WGS) analysis of bacterial isolates to support an epidemiological investigation. An epidemiological investigation led by the Infection Control Manager of the hospital and supported by an outbreak response team was conducted. Standard microbiological procedures were used to process stool samples and culture/identify diarrhoeal pathogens. Bacterial cultures were investigated using WGS performed using Illumina NextSeq technology, and WGS data were analysed using multiple bioinformatics tools, including those available at the Center for Genomic Epidemiology and EnteroBase. Core genome multilocus sequence typing (cgMLST) was used to investigate the phylogeny of isolates. Forty-nine cases were identified, with stool samples collected from 21 cases, and nontyphoidal <i>Salmonella</i> isolated from 19 out of 21 (90%) of the samples. All isolates were identified as <i>Salmonella enterica</i> serovar Enteritidis and differed from each other by ≤2 allele differences on cgMLST, indicating that isolates are highly genetically related. Delays in testing of food retention samples rendered the negative test results of limited value. A case-control study was conducted; eating chicken pasta was strongly associated with developing gastroenteritis (Odds Ration (OR) = 15.4, Chi-Square test with Yates correction <i>p</i> value = 0.02). The epidemiological evidence suggests that the chicken pasta was the likely vehicle of transmission in this outbreak, although the source of <i>S. enterica</i> serovar Enteritidis remains unknown.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11572493/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142670208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enterotoxigenic Escherichia coli in Blantyre, Malawi.","authors":"Philip M Ashton, Zefaniah Joel Katuah, Arnold Botomani, Belson M Kutambe, Nigel A Cunliffe, Astrid von Mentzer, Chisomo Msefula, Khuzwayo C Jere","doi":"10.1099/acmi.0.000885.v3","DOIUrl":"10.1099/acmi.0.000885.v3","url":null,"abstract":"<p><p>We announce the deposition of the first two enterotoxigenic <i>Escherichia coli</i> (ETEC) genomes from Malawi. They were isolated from the faeces of asymptomatically infected children obtained in 2014. Both genomes encode the porcine variant of the heat-labile toxin and no known ETEC colonization factors.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11572489/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142670207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic review and meta-analysis of the association between neglected tropical diseases and malnutrition: more research needed on diseases other than intestinal parasites, leishmaniasis and leprosy.","authors":"Aloysius Loglo, Wilfred Aniagyei, Monika Mira Vivekanandan, Abigail Agbanyo, Evans Adu Asamoah, Richard O Phillips, Reginald Annan, Barbara Engel, Rachel E Simmonds","doi":"10.1099/acmi.0.000800.v3","DOIUrl":"10.1099/acmi.0.000800.v3","url":null,"abstract":"<p><p><b>Background.</b> According to the World Health Organization, neglected tropical diseases (NTDs) affect over two billion people worldwide. While the links between nutrition and many diseases have become clear over recent decades, NTDs have lagged behind and the linkage with nutrition is largely unknown. We conducted this systematic review with meta-analysis to determine the current knowledge on the association between NTDs and malnutrition. <b>Methodology.</b> PubMed, Embase, Scopus and African Journals Online databases were searched using predefined search terms. We included all original articles with a case-control design and at least one NTD. The studies had to compare nutritional parameters between infected cases and control participants. Articles that did not report original data were excluded. The quality of the studies was assessed using the Newcastle-Ottawa scale. Pooled estimates were conducted using the random effect model. The publication bias of the studies was determined by funnel plots. <i>Q</i> and <i>I</i> ² statistics were used to assess the heterogeneity of the studies. <b>Results.</b> After screening 1294 articles, only 16 qualified for the systematic review and 12 for meta-analysis. These predominately had a focus on soil-transmitted helminthiasis (ascariasis, hookworm diseases and trichuriasis) and schistosomiasis, with a minority concerning leishmaniasis and leprosy. Pooled estimates showed an association between intestinal parasites and stunting in children [odds ratio (OR) = 1.38, 95% confidence interval (CI): 1.14-1.66, <i>I</i> ² = 0%, tau² = 0]. We also identified a moderate association established between serum iron deficiency (OR = 4.67, 95% CI: 1.91-11.44, tau² = 0) and intestinal parasites. <b>Conclusions/significance.</b> Of the 20 NTDs, the links between diet and disease have been explored for only 4. There is a paucity of data from low- and middle-income countries and least-developed countries where the NTD burden is high. Therefore, more research into the role of malnutrition in NTDs other than intestinal parasites, leishmaniasis and leprosy is needed.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11559247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell division cycle fluctuation of Pal concentration in Escherichia coli.","authors":"Laureen M Y Mertens, Xinwei Liu, Jolanda Verheul, Alexander J F Egan, Waldemar Vollmer, Tanneke den Blaauwen","doi":"10.1099/acmi.0.000759.v3","DOIUrl":"10.1099/acmi.0.000759.v3","url":null,"abstract":"<p><p>The Tol-Pal proteins stabilize the outer membrane during cell division in many Gram-negative bacteria, including <i>Escherichia coli</i>. Pal is an outer membrane lipoprotein that can bind peptidoglycan. It accumulates at the septum during division by a mobilization-and-capture mechanism. This work further substantiates and extends knowledge of Pal's localization in <i>E. coli</i> using immunolabelling; this method enables the detection of endogenous proteins. The midcell localization of Pal and TolB, as seen with fluorescent protein fusions, during cell division, was confirmed. The retention of Pal in newly formed cell poles seemed to persist longer than observed with fluorescent Pal fusions. The concentration of endogenous Pal during the cell division cycle fluctuated: it decreased initially (to half the fluorescence concentration (32.1 au µm^-3) of the maximum (64.1 au µm^-3) reached during the cell cycle) and then increased during the second half of the cell division cycle. We probed for possible regulators and proposed two new putative regulators of Pal. By deleting the periplasmic protease, Prc decreased the total Pal abundance (to ~65% of the fluorescence concentration in WT cells) and affected its concentration fluctuation during the cell cycle. This suggests that Prc controls a cell division stage-specific regulator of Pal. Immunolabelling also supported the prediction that the small RNA MicA suppresses Pal expression (the fluorescence concentration of Pal in cells without MicA is double that of Pal in WT cells). However, the regulation by MicA occurred in a cell cycle-independent manner. All these findings urge further research on the tight regulation of the dividing cell envelope stability.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11559426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial co-infections in mucormycosis in severely ill populations: an overlooked and complex challenge.","authors":"D E Corzo Leon, V H Ahumada-Topete, L Ostrosky-Zeichner","doi":"10.1099/acmi.0.000850.v4","DOIUrl":"https://doi.org/10.1099/acmi.0.000850.v4","url":null,"abstract":"<p><p>Mucormycosis is found in co-infection with bacteria in >50% of the cases. Most of these cases were reported among people with haematological diseases. The two most frequent bacteria found were <i>Pseudomonas aeruginosa</i> and <i>Klebsiella pneumoniae</i>. Almost 40% of the identified bacteria were reported as multidrug resistant.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characteristics of CTX-M β-lactamase-producing and quinolone-resistant Escherichia coli among deer in a popular tourist spot in Japan.","authors":"Shiori Ikushima, Michiyo Sugiyama, Tetsuo Asai","doi":"10.1099/acmi.0.000882.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000882.v3","url":null,"abstract":"<p><p><b>Introduction.</b> Antimicrobial resistance (AMR) is a growing global concern. Clonal lineages of CTX-M β-lactamase-producing <i>Escherichia coli</i> (CTXE) and quinolone-resistant <i>E. coli</i> (QREC) were disseminated among the deer population in a famous tourist destination (Nara Park; NP) in Japan. <b>Hypothesis/gap statement.</b> The molecular characteristics of CTXE or QREC isolates, which could pose a threat to public health, have not been elucidated. <b>Aim.</b> This study aimed to characterize the genetic traits of CTXE and QREC isolates derived from NP deer and compare them with lineages prevalent worldwide. <b>Methodology.</b> Sixteen CTXE and three QREC isolates recovered from NP deer faeces between 2018 and 2020 were analysed using whole-genome sequencing (WGS). For endemic lineages, phylogenetic trees were constructed against the isolates registered in the EnteroBase database using the core genome SNP scheme. <b>Results.</b> The most prevalent lineage in NP deer was ST3580. Several pandemic lineages, such as sequence type (ST) 38, ST58 and ST117, were included. The QREC lineages prevalent among deer were designated as extra-intestinal pathogenic <i>E. coli</i> or uropathogenic <i>E. coli</i> (UPEC). Thirteen of the 24 antimicrobial resistance genes (ARGs) were considered high-risk ARG families. Chromosomal integration of <i>bla</i> _CTX-M-15 was observed in all plasmid-negative isolates. Phylogenetic analysis suggested relationships between NP isolates and isolates sourced from the environment or poultry. <b>Conclusion.</b> ST3580 has a high potential for clonal dissemination. Furthermore, multiple clinically relevant lineages of CTXE and QREC are endemic in NP deer; however, they could be less virulent than isolates belonging to the same lineages, which could cause severe infectious diseases. Further studies are required to investigate the relationship between chromosomal integration of plasmid-encoded genes and the stable propagation of AMR bacteria in wildlife and the environment.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556432/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological and virological characteristics of people living with HIV on antiretroviral treatment for more than 6 months in virological failure in Brazzaville, Republic of Congo.","authors":"Ferdinand Emaniel Brel Got, Gervillien Arnold Malonga, Juthèce Privat Malanda-Kiminou, Maryse Akolbout, Ghislain Loubano-Mvoumbi, Dagene Ebourombi, Merlin Diafouka, Gontran Ondzotto","doi":"10.1099/acmi.0.000805.v3","DOIUrl":"10.1099/acmi.0.000805.v3","url":null,"abstract":"<p><p><b>Introduction.</b> Virological failure is one of the main causes of failing to treat, and better management of HIV infection requires understanding and controlling the factors that contribute to this phenomenon. The main objective was to characterize the patients of the active file of the Brazzaville Outpatient Treatment Center in virological failure to identify predictive factors leading to virological failure. <b>Methods.</b> Conducted between June and December 2020, this was a cross-sectional study. Patients enrolled were HIV-1-infected patients from the Brazzaville Outpatient Treatment Center receiving a potent combination therapy for at least 6 months but experiencing virological failure. Viral load was measured using the automated Abbott Real-time HIV-1 m2000rt System. Sociodemographic and clinical data were collected from a computerized patient record software called Santia. For the identification of the independent predictors of virological failure, statistical analysis was performed. <b>Results.</b> A total of 109 patients with virological failure were recruited. The median age of the patients was 45 years (interquartile range: 37-52 years) and women were more represented (74%). More than half of the patients had World Health Organization stage IV HIV and the median duration of antiretroviral treatment was 96 months. The most followed treatment regimen was AZT+3TC+EFV (or nevirapine) with 48%, while the median viral load was 12985 copies ml^-1. <b>Conclusion.</b> In our study, we did not identify any sociodemographic or clinical variables predictive of virological failure. However, we felt that it would be desirable to carry out a study with temporal follow-up and the possibility of sequencing in order to identify the different circulating genotypes and resistance mutations.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11652753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome sequence data for 61 isolates of Xanthomonas campestris pv. campestris from Brassica crops in Serbia.","authors":"Tatjana Popović Milovanović, Shannon Greer, Renata Iličić, Aleksandra Jelušić, Daisy Bown, Mikaeel Hussain, Jamie Harrison, Murray Grant, Joana G Vicente, David J Studholme","doi":"10.1099/acmi.0.000870.v3","DOIUrl":"10.1099/acmi.0.000870.v3","url":null,"abstract":"<p><p>This Technical Resource describes genome sequencing data for 61 isolates of the bacterial pathogen <i>Xanthomonas campestris</i> pv. <i>campestris</i> collected from <i>Brassica</i> and <i>Raphanus</i> crops between 2010 and 2021 in Serbia. We present the raw sequencing reads and annotated contig-level genome assemblies and determine the races of ten isolates. The data can be used to test hypotheses and phylogeographic analyses and inform the design of informative molecular markers for population genetics studies. When combined with phenotypic data, they could be used to dissect relationships between genotypes and phenotypes such as host range and virulence. Finally, these genome sequences expand our inventory of plasmids known to reside in this pathogen.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity of HBV genotypes and their association with precore/basal core mutations among HBsAg-positive patients in Ibadan, Nigeria.","authors":"Adedayo Omotayo Faneye, Babatunde Olanrewaju Motayo, Aisha Mustafa, Georgina Odiabo","doi":"10.1099/acmi.0.000821.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000821.v3","url":null,"abstract":"<p><p><b>Background.</b> Hepatitis B virus (HBV) is the most implicated cause of severe liver disease and hepatocellular carcinoma worldwide. Studies have shown that the basal core protein (BCP) and precore protein (PC) of HBV play a significant role in HBV-related carcinogenesis. There is a paucity of data on the type and effect of BCP and PC mutations in Nigeria. This study aims to genotype HBV and investigate any mutations within the BCP and PC among HBV patients in Ibadan, Nigeria. <b>Methods.</b> Forty HBV-DNA-positive patients were recruited into this study, and the viral load assay and genotyping by nested multiplex PCR were done. The partial X gene region was amplified and Sanger sequenced. The BPC and PC genomic regions were then analysed using bioinformatics. <b>Results.</b> Twenty-three participants recorded HBV DNA viral load of >20 000 IU, while 17 had <20 000 IU and 28 samples were genotyped. Five genotypes (A, B, C, D and E) and four mixed genotypes (AC, AD ACD and ABCD) were detected. Genotype AC was the most frequently encountered, while genotypes E and B were the least encountered. Mutation was highest in ages 34-45 years. Double mutation A1762T and G1764A within the BCP region was the most encountered mutation. <b>Conclusions.</b> We report a diverse HBV genetic landscape, with mixed infections between genotypes with BCP double-mutation A1762T/G1764A, signalling the likelihood of poor HBV-related liver disease prognosis. Our findings contribute to our understanding of the molecular characteristics of HBV and its potential implications for disease progression and management among HBV-infected Nigerians.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11542583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erysipelothrix rhusiopathiae-associated bloodstream infection in a patient with systemic lupus erythematosus: a case report and literature review.","authors":"Calvin Ka-Fung Lo, Cole Schonhofer, Neil Mina, Shazia Masud, Patrick Ho Pun Wong, Michael G Chapman","doi":"10.1099/acmi.0.000881.v3","DOIUrl":"10.1099/acmi.0.000881.v3","url":null,"abstract":"<p><p><b>Introduction.</b> Systemic human infections caused by <i>Erysipelothrix rhusiopathiae</i> have been increasingly reported especially within immunocompromised hosts and those with significant occupational exposure to livestock and aquatic animals. We report a case of <i>E. rhusiopathiae</i> bacteraemia in a patient with systemic lupus erythematosus (SLE) and present a literature review on clinical outcomes and microbiologic diagnosis for this organism. <b>Casepresentation.</b> A 43-year-old female patient was reporting a 1-month history of intermittent fevers. She recently increased her immunosuppression medication for her underlying SLE on the advice of her rheumatologist. The patient sustained a finger laceration from butchering cattle meat 2 weeks after the onset of her initial symptoms, with worsening index finger swelling and increased febrile episodes. Two weeks post-injury, multiple blood cultures were drawn, and each isolated Gram-positive bacilli. Given her recurrent intermittent fevers, there was a concern for ongoing infection, and therefore, intravenous vancomycin was started with prompt referral to an outpatient parenteral antibiotic therapy clinic. The Gram-positive bacillus was confirmed as <i>E. rhusiopathiae</i> via matrix-assisted laser desorption/ionization-time of flight analysis. Given intrinsic resistance to vancomycin, vancomycin was switched to intravenous ceftriaxone as targeted antimicrobial therapy for 2 weeks. Reassuringly, there was no echocardiographic evidence of infective endocarditis, warranting the prolonged treatment course. Post-treatment, she remained symptom-free with the resolution of joint symptoms and fevers. <b>Conclusion.</b> Our report illustrates a case of <i>E. rhusiopathiae</i> bacteraemia from an immunodeficient host, with prompt microbiologic diagnosis and intervention with appropriate antimicrobial coverage. Literature reflects the rarity of this infection, predilections to specific susceptible hosts and the importance of raising awareness of zoonotic infections.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11649197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}