Access microbiology最新文献

{"title":"Retrospective analysis of Acinetobacter baumannii bacteraemia risk factors, complications and mortality in a tertiary university hospital in Saudi Arabia.","authors":"Reham Kaki","doi":"10.1099/acmi.0.000826.v4","DOIUrl":"10.1099/acmi.0.000826.v4","url":null,"abstract":"<p><p><b>Introduction.</b> There are many multidrug-resistant isolates of the nosocomial pathogen, <i>Acinetobacter baumannii</i>, causing severe healthcare-acquired infections in terminally ill patients with high mortality and morbidity rates. <b>Aim.</b> This study aims to retrospectively analyse <i>A. baumannii</i> bacteraemia (ABB) cases in Saudi Arabia, where the information is sparse regarding the prevalence, risk factors, clinical disease, antibiotic regimen, antibiotic susceptibility, treatment outcomes and mortality associated with this infection. <b>Methods.</b> A retrospective chart review was conducted between 1 January 2015 and 31 December 2022 to identify all patients aged 14 years and above with ABB. Demographic and clinical data, as well as results from laboratory analyses, were collected from patients' electronic charts. Statistical analyses were performed on the data to identify factors associated with 90-day mortality. <b>Results.</b> Of the 122 ABB cases, 71 (63.4%) died. The factors that were found to be associated with 90-day mortality were the Charlson Comorbidity Index, Pitt bacteraemia score, quick Sequential Organ Failure Assessment score (<i>P</i><0.001 for each), hospital ward (<i>P</i><0.02), short duration of antibiotic treatment (<i>P</i><0.01) and higher age (<i>P</i><0.05). The most common source of infection was central line-associated bloodstream infection in 52.7%. Also associated with mortality were inappropriate antimicrobial therapy (<i>P</i><0.02) and empirical use of colistin (<i>P</i><0.05). In many patients, ABB was caused by carbapenem-resistant <i>A. baumannii</i> [(CRAB), 69.6%], and 74.4% of those patients died. <b>Conclusion.</b> To prevent ABB-associated mortality, an appropriate regimen and duration of treatment are necessary. Hospitals should also practice proper hygiene to prevent the spread of ABB. CRAB is a growing threat in hospitals in Saudi Arabia, especially in the critical care setting, and carries a very high risk of mortality.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Salmonella enterica biovars Gallinarum and Pullorum and their antibiotic resistance pattern in integrated crop-livestock farms and poultry meats.","authors":"Dita Julianingsih, Zabdiel Alvarado-Martinez, Zajeba Tabashsum, Chuan-Wei Tung, Arpita Aditya, Sarika Kapadia, Saloni Maskey, Aditi Mohapatra, Debabrata Biswas","doi":"10.1099/acmi.0.000775.v6","DOIUrl":"10.1099/acmi.0.000775.v6","url":null,"abstract":"<p><p>Due to consumer demand, many conventional poultry farms are now growing poultry without antibiotics or synthetic chemicals. In addition to this, pasture/organic poultry farms have increased significantly in the USA, and they are also antibiotic- and chemical-free. According to recent reports, both antibiotic-free conventional and pasture poultry farmers are facing the re-emergence of bacterial diseases. Bacterial diseases cause higher mortality rates in birds and lead to non-profitable poultry farming. This study investigated the prevalence of <i>Salmonella enterica</i> subsp. <i>enterica</i> serovar Gallinarum biovars Gallinarum (<i>S</i>. Gallinarum), the causative agent of fowl typhoid, and <i>Salmonella enterica</i> subsp. <i>enterica</i> serovar Gallinarum biovars Pullorum (<i>S</i>. Pullorum), the causative agent of pullorum disease, within integrated crop-livestock/pasture farm environments and their processed products. Specifically, the study focused on both the pre-harvest period, which includes the conditions and practices on the farm before the crops and livestock are harvested, and the post-harvest period, which encompasses the handling, processing, and storage of the products after harvest. A total of 1286 samples were collected from six farms and adjacent 13 markets to determine the prevalence of <i>S</i>. Gallinarum and <i>S</i>. Pullorum by using both microbiological culture and molecular techniques, specifically PCR. Antimicrobial susceptibility testing was performed using the agar dilution method for the recommended antibiotics as described in the Clinical Laboratory Standards Institute (CLSI). <i>S</i>. Pullorum was detected in 11 samples (2.7%), while <i>S</i>. Gallinarum was found in six samples (1.5%) out of a total of 403 samples at the pre-harvest level. At the post-harvest level, only <i>S</i>. Gallinarum was identified in 14 meat samples out of 883(1.6%) recovered from samples collected from retail markets. Antibiogram showed <i>S</i>. Gallinarum and <i>S</i>. Pullorum to be highly resistant to cephradine, trimethoprim-sulfamethoxazole, amoxicillin, streptomycin, and ampicillin. This data demonstrates that both <i>S</i>. Pullorum and <i>S</i>. Gallinarum are commonly present in farm poultry environments as well as the products sold in the markets, which warrants implementation of regular surveillance and monitoring programmes, as well as potentially requiring future control strategies to reduce <i>S</i>. Pullorum and <i>S</i>. Gallinarum transmission.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11652723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome sequences of marine bacteria presenting the ability to promote the growth of the diatom Phaeodactylum tricornutum.","authors":"Rodrigo Martins, Constança D F Bertrand, Francisco Quintas-Nunes, Pedro Reynolds-Brandão, Maria T Barreto Crespo, Francisco X Nascimento","doi":"10.1099/acmi.0.000864.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000864.v3","url":null,"abstract":"<p><p>We describe the whole-genome sequences of seven diverse marine bacteria isolated from Portuguese environments that presented the ability to promote the growth of the model diatom, <i>Phaeodactylum tricornutum</i>. The bacterial genome sequences will contribute to the study of genetic and molecular mechanisms involved in diatom-bacteria interactions.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11432598/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Y498T499-SARS-CoV-2 spike (S) protein interacts poorly with rat ACE2 and does not affect the rat lung.","authors":"Amy L Green, Dylan De Bellis, Evangeline Cowell, Roman V Lenchine, Timothy Penn, Luke P Kris, James McEvoy-May, Shailesh Bihari, Dani-Louise Dixon, Jillian M Carr","doi":"10.1099/acmi.0.000839.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000839.v3","url":null,"abstract":"<p><p>The rat is a useful laboratory model for respiratory diseases. SARS-CoV-2 proteins, such as the spike (S) protein, can induce inflammation. This study has investigated the ability of the Q498Y, P499T (QP-YT) amino acid change, described in the S-protein of the mouse-adapted laboratory SARS-CoV-2 MA strain, to interact with rat angiotensin converting enzyme-2 (ACE2) and stimulate responses in rat lungs. A real-time S-ACE2 quantitative fusion assay shows that ancestral and L452R S-proteins fuse with human but not rat ACE2 expressed on HEK293 (human embryonic kidney-293) cells. The QP-YT S-protein retains the ability to fuse with human ACE2 and increases the binding to rat ACE2. Although lower lung of the rat contains both ACE2 and TMPRSS2 (transmembrane serine protease 2) target cells, intratracheal delivery of ancestral or QP-YT S-protein pseudotyped lentivirus did not induce measurable respiratory changes, inflammatory infiltration or innate mRNA responses. Isolation of primary cells from rat alveoli demonstrated the presence of cells expressing ACE2 and TMPRSS2. Infection of these cells, however, with ancestral or QP-YT S-protein pseudotyped lentivirus was not observed, and the QP-YT S-protein pseudotyped lentivirus poorly infected HEK293 cells expressing rat ACE2. Analysis of the amino acid changes across the S-ACE2 interface highlights not only the Y498 interaction with H353 as a likely facilitator of binding to rat ACE2 but also other amino acids that could improve this interaction. Thus, rat lungs contain cells expressing receptors for SARS-CoV-2, and the QP-YT S-protein variant can bind to rat ACE2, but this does not result in infection or stimulate responses in the lung. Further, amino acid changes in S-protein may enhance this interaction to improve the utility of the rat model for defining the role of the S-protein in driving lung inflammation.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11432600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of soil DNA extraction kits for long read metagenomic sequencing.","authors":"Harry T Child, Lucy Wierzbicki, Gabrielle R Joslin, Richard K Tennant","doi":"10.1099/acmi.0.000868.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000868.v3","url":null,"abstract":"<p><p>Metagenomics has been transformative in our understanding of the diversity and function of soil microbial communities. Applying long read sequencing to whole genome shotgun metagenomics has the potential to revolutionise soil microbial ecology through improved taxonomic classification, functional characterisation and metagenome assembly. However, optimisation of robust methods for long read metagenomics of environmental samples remains undeveloped. In this study, Oxford Nanopore sequencing using samples from five commercially available soil DNA extraction kits was compared across four soil types, in order to optimise read length and reproducibility for comparative long read soil metagenomics. Average extracted DNA lengths varied considerably between kits, but longer DNA fragments did not translate consistently into read lengths. Highly variable decreases in the length of resulting reads from some kits were associated with poor classification rate and low reproducibility in microbial communities identified between technical repeats. Replicate samples from other kits showed more consistent conversion of extracted DNA fragment size into read length and resulted in more congruous microbial community representation. Furthermore, extraction kits showed significant differences in the community representation and structure they identified across all soil types. Overall, the QIAGEN DNeasy PowerSoil Pro Kit displayed the best suitability for reproducible long-read WGS metagenomic sequencing, although further optimisation of DNA purification and library preparation may enable translation of higher molecular weight DNA from other kits into longer read lengths. These findings provide a novel insight into the importance of optimising DNA extraction for achieving replicable results from long read metagenomic sequencing of environmental samples.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11432601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disseminated hydatidosis an unusual presentation: a case report.","authors":"Fatima Ziad, Imane Zouaoui, Mostaine El Mamoune, Sarra Aoufi","doi":"10.1099/acmi.0.000803.v3","DOIUrl":"10.1099/acmi.0.000803.v3","url":null,"abstract":"<p><p>Hydatidosis, also known as cystic echinococcosis, is a widespread zoonosis, caused by a tapeworm of the genus <i>Echinococcus</i>. It presents a significant public health concern, particularly in endemic areas. The occurrence of disseminated hydatid disease is uncommon, even in regions where it is endemic, with an incidence ranging from 1-8%. The definitive diagnosis relies on a parasitological method. In this work, we present an unusual case of disseminated hydatid disease that was diagnosed in the central parasitology-mycology laboratory of 'The Ibn Sina University Hospital'. This is a 21-year-old patient residing in a rural area, who presented with heaviness-type pain in the right hypochondrium, accompanied with nausea and vomiting. During the examination, the patient mentioned the contact with dogs. Abdominal radiography (ultrasound and CT) revealed findings suggestive of multiple hydatid cysts located in the liver and peritoneum. This suspicion was confirmed by positive hydatid serology. After 9 months of treatment with albendazole, the patient underwent surgery for excision of the cysts shown on the x-ray, as well as other cysts incidentally discovered intraoperatively at the pelvic and rectal levels. All of the extracted specimens were sent to the parasitology laboratory. The direct examination, along with the viability test, revealed the presence of hooks and scolex of non-viable <i>Echinococcus granulosus</i>. Disseminated hydatidosis is a rare but serious presentation, and the positive diagnosis relies on several epidemiological, clinical, radiological and parasitological arguments. Medical and surgical treatments play a crucial role in determining the patient's prognosis.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142309575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends and antibiotic susceptibility patterns of diarrhoeal pathogens - experience over 14 years in southern India.","authors":"Ankita Mohanty, Nayannika Lakra, Jharna Mandal","doi":"10.1099/acmi.0.000818.v3","DOIUrl":"10.1099/acmi.0.000818.v3","url":null,"abstract":"<p><p><b>Introduction</b>. Enteric pathogens contribute significantly to morbidity in a developing country such as India. Early and prompt diagnosis of diarrhoeal diseases can reduce the mortality rate, particularly in children. The pattern of sensitivity to antimicrobials for the common pathogens can vary from time to time. The present study was conducted to study the pathogen distribution and antimicrobial susceptibility pattern during the study period (January 2010 to December 2023). <b>Hypothesis/gap statement</b>. Studying the changing trend in the antimicrobial sensitivity pattern of diarrhoeal pathogens over a decade can help to plan future treatment options. <b>Aim</b>. This study was undertaken to provide insights into the changing pattern of pathogen distribution and antimicrobial susceptibility for enteric pathogens over 14 years. <b>Methods</b>. A retrospective observational cohort analysis was conducted on all the stool pathogens isolated from the samples received in the microbiology department of a tertiary care hospital from 2010 to 2023. The demographic details, stool microscopy, culture reports, and antimicrobial susceptibility patterns were noted. <b>Results</b>. A total of 18 336 stool specimens were received in the microbiology laboratory between January 2010 and December 2023, of which 1354 specimens had diarrhoeal pathogens grown in culture. Out of these 1354 specimens, 591 (44%) had <i>Salmonella</i>, 471 (35%) <i>Shigella</i>, 181 (13%) <i>Vibrio cholerae,</i> and 80 (6%) <i>Aeromonas</i> species. Among these pathogens, susceptibility to ceftriaxone was seen in 93% (552 isolates) of <i>Salmonella</i> species, 89% (420 isolates) of <i>Shigella</i> species, and 95% (171 isolates) of <i>Vibrio cholerae</i>; 91% (73 isolates) of <i>Aeromonas</i> species were susceptible to chloramphenicol. Some major parasites were also observed on microscopy. <b>Conclusion</b>. Timely diagnosis of diarrhoeal pathogens can be life-saving for patients at the extremes of age, i.e. in children and the elderly. Pathogens can exhibit a changing susceptibility pattern to antibiotics, which should be regularly observed to plan future therapy.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142309576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of an optimal agar medium for detecting hypervirulent Klebsiella pneumoniae using string test.","authors":"Naoki Watanabe, Akari Masuda, Tomohisa Watari, Yoshihito Otsuka, Kazufumi Yamagata, Miyuki Fujioka","doi":"10.1099/acmi.0.000834.v3","DOIUrl":"10.1099/acmi.0.000834.v3","url":null,"abstract":"<p><p>The string test is a screening method for detecting hypervirulent <i>Klebsiella pneumoniae</i> (hvKp). Agar media are used for string tests; however, the effect of the type of media on the test results remains unclear. We aimed to determine the optimal agar medium and cutoff value for the string test. We performed the string test on 99 <i>Klebsiella</i> strains using different agar media: sheep blood, chocolate, Drigalski's and MacConkey. The diagnostic accuracy was calculated in concordance with the <i>rmpA</i>, <i>rmpA2</i> or <i>iucA</i> gene levels. The diagnostic accuracy rates for sheep blood, chocolate, Drigalski's and MacConkey agar were 0.79, 0.75, 0.73 and 0.64, respectively. When the cutoff was changed from 5 to 10 mm, the diagnostic accuracy rate for sheep blood agar decreased from 0.79 to 0.65. Our findings suggest that the type of agar medium impacts the string test results and sheep blood agar with a 5-mm cutoff is the optimal condition for detecting hvKp.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11652848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genome analyses of Staphylococcus aureus from platelet concentrates reveal rearrangements involving loss of type VII secretion genes.","authors":"Sylvia Ighem Chi, Annika Flint, Kelly Weedmark, Franco Pagotto, Sandra Ramirez-Arcos","doi":"10.1099/acmi.0.000820.v4","DOIUrl":"10.1099/acmi.0.000820.v4","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> has been involved in transfusion-transmitted fatalities associated with platelet concentrates (PCs) due to its heightened pathogenicity enhanced by genome-encoded virulence and antibiotic resistance genes. This may be facilitated by mobile genetic elements (MGEs) that can cause rearrangements. Several factors contribute to <i>S. aureus</i> virulence, including the type VII secretion system (T7SS), composed of six core genes conserved across <i>S. aureus</i> strains. In this study, we conducted comparative genome analyses of five <i>S. aureus</i> isolates from PCs (CI/BAC/25/13 /W, PS/BAC/169/17 /W and PS/BAC/317/16 /W were detected during PCs screening with the BACT/ALERT automated culture system, and ATR-20003 and CBS2016-05 were missed during screening and caused septic transfusion reactions). Multiple alignments of the genomes revealed evidence of rearrangements involving phage Sa3int in PS/BAC/169/17 /W and PS/BAC/317/16 /W. While the former had undergone translocation of its immune evasion cluster (IEC), the latter had lost part of the phage, leaving behind the IEC. This observation highlights <i>S. aureus</i> genome plasticity. Unexpectedly, strain CBS2016-05 was found to encode a pseudo-type VII secretion system (T7SS) that had lost five of the conserved core genes (<i>esxA</i>, <i>esaA, essA, esaB</i> and <i>essB</i>) and contained a 5' truncated <i>essC</i>. Since these genes are essential for the function of the T7SS protein transport machinery, which plays a key role in <i>S. aureus</i> virulence, CBS2016-05 probably compensates by recruiting other export mechanisms and/or alternative virulence factors, such as neu-tralizing immunity proteins. This study unravels genome rearrangements in <i>S. aureus</i> isolated from PCs and reports the first <i>S. aureus</i> isolate lacking conserved T7SS core genes.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11652724/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aspergillus oesophagitis in a patient with solid tumors: a case report.","authors":"Vanessa Abi Rached, Karine Azar, Sarah Gerges, Souheil Hallit, Elie Akoury, Georges Chahine, Rabih Hallit, Bassem Akiki, Rita Rizk","doi":"10.1099/acmi.0.000842.v4","DOIUrl":"https://doi.org/10.1099/acmi.0.000842.v4","url":null,"abstract":"<p><p>Oesophageal aspergillosis is a rare occurrence primarily documented in hematologic malignancies, and only rarely occurring among patients with solid tumours. In this case report, we present the unique case of an 81-year-old Lebanese man who had a remarkable medical history, including four solid tumours. The patient sought medical attention due to dysphagia and weight loss, prompting a gastroscopic examination that revealed a necrotic abscess at the oesophagogastric junction. Initial treatment with fluconazole and esomeprazole was administered, but the recurrence of similar symptoms led to a repeat gastroscopy, unveiling a diagnosis of <i>Aspergillus</i> oesophagitis. Intravenous voriconazole was promptly initiated; however, the patient developed a significant pericardial effusion and expired, with <i>Aspergillus</i> species identified in the pericardial fluid prior to patient expiring. This exceptional case emphasizes the importance of considering oesophageal aspergillosis in cancer patients who present with refractory symptoms such as epigastric pain, dysphagia, nausea, and vomiting, despite symptomatic treatment. Our findings underscore the need for increased awareness and the inclusion of gastrointestinal endoscopy as part of the diagnostic approach for this rare but potentially life-threatening condition.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11397932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142305495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}