社区和医院分离鲍曼不动杆菌对HeLa细胞系和昆虫模型mellongalleria的毒力比较。

Access microbiology Pub Date : 2025-02-14 eCollection Date: 2025-01-01 DOI:10.1099/acmi.0.000858.v3
Nazmul Hasan Muzahid, Aarthi Ramesh, Tan Hock Siew, Md Zobaer Hasan, Kumaran Narayanan, Sadequr Rahman
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引用次数: 0

摘要

鲍曼不动杆菌是一种重要的医院病原菌,在感染人群中具有很高的感染率和发病率,大多数研究都集中在医院感染菌株上。然而,鲍曼不动杆菌也可以从健康的社区个体中分离出来。本研究比较了医院和社区分离的鲍曼不动杆菌的致病性,使用了mellonella Galleria和人类细胞培养。采用昆虫模型、大mellonella和体外HeLa细胞系与10株鲍曼不动杆菌分离株(来自马来西亚Segamat的6株社区和4株医院分离株)进行研究。对分离株的毒力和侵袭力进行了研究,并对菌株进行了杀灭试验和HeLa细胞粘附、侵袭和细胞毒性试验。在调查的10个分离株中,发现3个社区和2个医院分离株在大黄蜂感染模型中具有高毒性,在96 h内杀死100%的幼虫。这些菌株也被发现具有侵袭性,并对HeLa细胞具有显著的细胞毒性。我们的研究表明,社区和医院分离的鲍曼不动杆菌在两种模型系统中都具有同样的毒性。毫无疑问,除了医院环境外,社区水库中存在的高毒力鲍曼不动杆菌对公共卫生构成重大风险,需要进一步调查。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of the virulence of community- and hospital- isolated Acinetobacter baumannii in HeLa cell line and insect model, Galleria mellonella.

Acinetobacter baumannii is an important nosocomial pathogen causing high infections and morbidity among affected individuals, and most studies focus on nosocomial strains. However, A. baumannii can also be isolated from healthy community individuals. This study compared the pathogenicity of hospital and community A. baumannii isolates using Galleria mellonella and human cell cultures. The insect model, G. mellonella, and in vitro HeLa cell line were used with ten A. baumannii isolates (six community and four hospital isolates from Segamat, Malaysia). G. mellonella killing assays and HeLa cell adherence, invasion and cytotoxicity assays were performed to investigate the virulence and invasion potential of the isolates. Out of the ten isolates investigated, three community and two hospital isolates were found to be highly virulent in the G. mellonella infection model, killing 100% of larvae within 96 h. These strains were also found to be invasive and have significant cytotoxicity in HeLa cells. Our study revealed that community- and hospital-isolated A. baumannii could be equally virulent judged by both model systems. Undoubtedly, besides hospital settings, the presence of highly virulent A. baumannii in community reservoirs poses a significant public health risk and requires additional investigation.

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