Access microbiology最新文献_第9页

Genome sequence of the plant-growth-promoting bacterium Bacillus velezensis EU07. 促进植物生长的芽孢杆菌 EU07 的基因组序列。

Access microbiology Pub Date : 2024-05-30 eCollection Date: 2024-01-01 DOI: 10.1099/acmi.0.000762.v3

Ömür Baysal, David J Studholme, Catherine Jimenez-Quiros, Mahmut Tör

引用次数: 0

Exceptional association of two species of bacteria causing acute appendicitis: Haemophilus influenzae and Enterobacter cloacae. 引起急性阑尾炎的两种细菌：流感嗜血杆菌和泄殖腔肠杆菌的特殊关联。

Access microbiology Pub Date : 2024-05-28 eCollection Date: 2024-01-01 DOI: 10.1099/acmi.0.000794.v3

Fatima Zahra Adil, Elmostafa Benaissa, Yassine Ben Lahlou, Leila Laamara, Fatna Bssaibis, Adil Maleb, Mariama Chadli, Mostafa Elouennass

引用次数: 0

Corrigendum: The Enterococcus secretome inhibits the growth of Mycobacterium tuberculosis complex mycobacteria. 更正：肠球菌分泌物抑制结核分枝杆菌复合体分枝杆菌的生长。

Access microbiology Pub Date : 2024-05-22 eCollection Date: 2024-01-01 DOI: 10.1099/acmi.0.000843

Wafaa Achache, Jean Louis Mege, Mustapha Fellag, Michel Drancourt

引用次数: 0

Brief Report: Nasal colonization with Staphylococcus aureus and methicillin resistant Staphylococcus aureus among community-dwelling older adults with comorbidities seeking follow-up medical care in Central Sri Lanka. 简要报告：斯里兰卡中部在社区居住并有合并症的老年人鼻腔定植金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌。

Access microbiology Pub Date : 2024-05-21 eCollection Date: 2024-01-01 DOI: 10.1099/acmi.0.000724.v3

Ifaaz Iqbal, Zeenath Sabri, Adithya Illangasinghe, Ashini Isurindi, Rashmi Jayakodi, Wasana Jayasekara, Kaveesha Jayarathna, Nonduni Jayasinghe, Mekhala Ishani, Ishan Jayasekara, Nelum Handapangoda, Dilrukshi Menike, Rasadanie Dissanayake, Asela Ekanayake, Veranja Liyanapathirana

{"title":"Brief Report: Nasal colonization with Staphylococcus aureus and methicillin resistant Staphylococcus aureus among community-dwelling older adults with comorbidities seeking follow-up medical care in Central Sri Lanka.","authors":"Ifaaz Iqbal, Zeenath Sabri, Adithya Illangasinghe, Ashini Isurindi, Rashmi Jayakodi, Wasana Jayasekara, Kaveesha Jayarathna, Nonduni Jayasinghe, Mekhala Ishani, Ishan Jayasekara, Nelum Handapangoda, Dilrukshi Menike, Rasadanie Dissanayake, Asela Ekanayake, Veranja Liyanapathirana","doi":"10.1099/acmi.0.000724.v3","DOIUrl":"10.1099/acmi.0.000724.v3","url":null,"abstract":"Older adults are more severely affected by infections caused by drug-resistant bacteria including Methicillin-Resistant Staphylococcus aureus (MRSA). We aimed to identify the MRSA colonization rates and associated factors among older adults aged more than 65-years-old. Among the 309 recruited, 152 (49.2 %) were males. Self-collected nasal swabs were used to isolate Staphylococcus aureus and MRSA with routine microbiological methods. Staphylococcus aureus was isolated from 36 (11.7 %) participants while 11 (3.6 %) were colonized with MRSA. We identified a significant association between the male sex and MRSA colonization (P=0.028, Chi-square test). However, this needs careful interpretation given the smaller number of outcome events. Other factors studied had no statistically significant association with MRSA colonization.","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11316518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141918499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of selective isolation media for detecting the genera Actinomyces and Schaalia from oral specimens containing indigenous bacteria 开发用于从含有本地细菌的口腔标本中检测放线菌属和沙雷菌属的选择性分离培养基

Access microbiology Pub Date : 2024-05-10 DOI: 10.1099/acmi.0.000768.v3

Sadao Aoki, Hiroko Yahara, Ryoma Nakao, Osamu Tsuzukibashi, K. Yahara

{"title":"Development of selective isolation media for detecting the genera Actinomyces and Schaalia from oral specimens containing indigenous bacteria","authors":"Sadao Aoki, Hiroko Yahara, Ryoma Nakao, Osamu Tsuzukibashi, K. Yahara","doi":"10.1099/acmi.0.000768.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000768.v3","url":null,"abstract":"To isolate specific bacteria from samples constituting the microbiota, it is essential to employ selective media that suppress the growth of resident bacteria other than specific target bacteria. Selective media for clinically important Actinomyces (including Schaalia, which was previously taxonomically classified as part of the genus Actinomyces) have been limited because they have been designed for a limited range of species within the genus and require ingredients which are difficult to prepare and handle. This study aimed to develop a selective medium [referred to as Actinomyces and Schaalia Selective Medium (ASSM)] for the isolation of a broad range of Actinomyces and Schaalia species from samples mixed with resident bacteria. The composition of ASSM includes yeast extract, agar, brain heart infusion (BHI), levofloxacin (LVFX), fosfomycin (FOM), colistin (CL) and metronidazole (MNZ). Evaluation of the medium using 24 swab samples serially collected from the roots of the teeth of a healthy individual for whom metagenome sequencing data of a saliva sample are publicly available revealed that ASSM adjusted to concentrations of LVFX 0.5 mg l−1, FOM 5 mg l−1, CL 1 mg l−1 and MNZ 2 mg l−1 and cultured anaerobically at 35 °C for 7 days enabled the isolation of Actinomyces species from 37.5 % of the samples. The inclusion of CL and MNZ in ASSM can also be useful for samples harbouring other bacterial species. The selective isolation medium is expected to contribute to studies investigating the relationship between these bacteria and their pathogenesis or disease.","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":" 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140993469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kaposi’s sarcoma herpesvirus viral FLICE inhibitory protein modulates A20 deubiquitinase activity 卡波西肉瘤疱疹病毒FLICE抑制蛋白调节A20去泛素化酶的活性

Access microbiology Pub Date : 2024-05-09 DOI: 10.1099/acmi.0.000625.v4

Kevin Herold, Ayana Ruffin, Jennifer C. Chmura, Anna J Dellomo, Elana S. Ehrlich

引用次数: 0

Clinical presentation and outcome of enteric fever in adult patients with cancer: a perspective from Pakistan 成年癌症患者肠热病的临床表现和预后：来自巴基斯坦的视角

Access microbiology Pub Date : 2024-05-01 DOI: 10.1099/acmi.0.000719.v3

Seemal Aslam, Salma Abbas, S. Nizamuddin, Muhammad Shehbaz, Azra Parveen, Faisal Sultan, Aun Raza

{"title":"Clinical presentation and outcome of enteric fever in adult patients with cancer: a perspective from Pakistan","authors":"Seemal Aslam, Salma Abbas, S. Nizamuddin, Muhammad Shehbaz, Azra Parveen, Faisal Sultan, Aun Raza","doi":"10.1099/acmi.0.000719.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000719.v3","url":null,"abstract":"\u0000 Introduction. Enteric fever is a significant health concern in endemic countries. While extensive research has been conducted to understand its presentation and outcomes in non-cancer patients, limited data exist on its impact on cancer patients. This descriptive study aims to investigate the clinical presentation and outcome in cancer patients.\u0000 \u0000 Methodology. This retrospective observational study analysed 90 adult cancer patients from a single centre in Pakistan from January 2017 to December 2022. Inclusion criteria involved documented blood culture infections with Salmonella typhi or paratyphi A, B, or C. We examined clinical presentation, laboratory parameters, antimicrobial resistance, complications, and outcomes. Additionally, we explored the effects of chemotherapy, comorbidities, type of malignancy, and patient age on complications and mortality.\u0000 \u0000 Results.\u0000 Salmonella typhi was the most prevalent organism (72.2 %), followed by Salmonella paratyphi A (22.2 %) and B (5.5 %). Variably-resistant isolates constituted 51.5 %, multi-drug resistant (MDR) isolates accounted for 20 %, extensively drug-resistant (XDR) for 14.4 % and ESBL-producers for 15.5 %, of all enteric fever infections. Enteric fever-associated complications were observed in 21.1 % of cases. Chemotherapy in the preceding month did not affect mortality, nor did age, gender, or malignancy type. However, comorbidities were statistically significant for mortality (p-value 0.03). A total of 8.8 % of patients required ICU care, and the all-cause 30 day mortality rate was 13.3 %\u0000 \u0000 Conclusion. Enteric fever remains prevalent in our geographical region. Unlike non-typhoidal Salmonella (NTS), enteric fever does not behave differently in an immunocompromised population, including cancer patients.","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"54 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141033015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An improved genome editing system for Sphingomonadaceae 改进的鞘氨醇单胞菌科基因组编辑系统

Access microbiology Pub Date : 2024-05-01 DOI: 10.1099/acmi.0.000755.v3

Inmaculada García-Romero, Rubén de Dios, Francisca Reyes-Ramírez

{"title":"An improved genome editing system for Sphingomonadaceae","authors":"Inmaculada García-Romero, Rubén de Dios, Francisca Reyes-Ramírez","doi":"10.1099/acmi.0.000755.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000755.v3","url":null,"abstract":"The sphingomonads encompass a diverse group of bacteria within the family Sphingomonadaceae, with the presence of sphingolipids on their cell surface instead of lipopolysaccharide as their main common feature. They are particularly interesting for bioremediation purposes due to their ability to degrade or metabolise a variety of recalcitrant organic pollutants. However, research and development on their full bioremediation potential has been hampered because of the limited number of tools available to investigate and modify their genome. Here, we present a markerless genome editing method for Sphingopyxis granuli TFA, which can be further optimised for other sphingomonads. This procedure is based on a double recombination triggered by a DNA double-strand break in the chromosome. The strength of this protocol lies in forcing the second recombination rather than favouring it by pressing a counterselection marker, thus avoiding laborious restreaking or passaging screenings. Additionally, we introduce a modification with respect to the original protocol to increase the efficiency of the screening after the first recombination event. We show this procedure step by step and compare our modified method with respect to the original one by deleting ecfG2, the master regulator of the general stress response in S. granuli TFA. This adds to the genetic tool repertoire that can be applied to sphingomonads and stands as an efficient option for fast genome editing of this bacterial group.","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"88 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141052450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peritoneal tuberculosis, an underestimated diagnosis: a case report 腹膜结核，一个被低估的诊断：病例报告

Access microbiology Pub Date : 2024-05-01 DOI: 10.1099/acmi.0.000753.v3

Leila Laamara, E. Benaissa, Amine Achemlal, Amal Bounakhla, F. Bssaibis, Y. Benlahlou, Adil Maleb, Mariama Chadli, Mostafa Elouennass

引用次数: 0

Microbiota of healthy dogs demonstrate a significant decrease in richness and changes in specific bacterial groups in response to supplementation with resistant starch, but not psyllium or methylcellulose, in a randomized cross-over trial 在一项随机交叉试验中，健康狗的微生物群对补充抗性淀粉（而非洋车前子或甲基纤维素）的反应显示出丰富度的显著下降和特定细菌群的变化

Access microbiology Pub Date : 2024-05-01 DOI: 10.1099/acmi.0.000774.v4

S. Salavati Schmitz, Jorge Perez-Accino Salgado, Laura Glendinning

引用次数: 0