{"title":"Spectrum of respiratory viruses identified from SARS-CoV-2-negative human respiratory tract specimens in Watansoppeng, Indonesia.","authors":"Irfan Idris, Isra Wahid, Ungke Antonjaya, Edison Johar, Fiqry Hasan Kleib, Ida Yus Sriyani, Aghnianditya Kresno Dewantari, Oderna Daming, Mustakim Duharing, Fatmawati Sappe, Hajar Hasan, Frilasita Aisyah Yudhaputri, Din Syafruddin, Khin Saw Aye Myint","doi":"10.1099/acmi.0.000840.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000840.v3","url":null,"abstract":"<p><p>Respiratory infections account for millions of hospital admissions worldwide. The aetiology of respiratory infections can be attributed to a diverse range of pathogens including viruses, bacteria and fungi. SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2)-negative specimens from Wattansoppeng city, South Sulawesi, were analysed to study the spectrum of respiratory viruses. Samples were screened for influenza virus, enterovirus, Paramyxoviridae, Nipah virus, Coronaviridae and Pneumoviridae. Of 210 specimens, 19 were positive for respiratory syncytial virus (RSV)-A, RSV-B, human parainfluenza virus type 1 (HPIV-1), HPIV-2, human rhinovirus (HRV)-A, HRV-B, HRV-C, human metapneumovirus (HMPV), influenza A virus (IAV) and coxsackievirus A6 (CV-A6). Influenza virus was of seasonal H3N2 subtype. The HMPVs were of genotypes B1 and A2a, while one RSV-A was of the ON-1 genotype. The viruses mostly affected children with unknown severity.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11469065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142485097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2024-10-11eCollection Date: 2024-01-01DOI: 10.1099/acmi.0.000786.v3
Jingjie Zhang, Huricha Baigued, Shana Chen, Haiyan Borigen, Tana Tana, Fu Quan, Dezhi Yang
{"title":"Bioinformatics analysis of the antigenic epitopes of L7/L12 protein in the B- and T-cells active against Brucella melitensis.","authors":"Jingjie Zhang, Huricha Baigued, Shana Chen, Haiyan Borigen, Tana Tana, Fu Quan, Dezhi Yang","doi":"10.1099/acmi.0.000786.v3","DOIUrl":"10.1099/acmi.0.000786.v3","url":null,"abstract":"<p><p>The objective is to analyse the physicochemical properties, spatial structure and protein-protein interactions (PPIs) of L7/L12 protein using bioinformatics methods and predict their B- and T-cell epitopes to lay a theoretical foundation for developing a novel multiepitope vaccine (MEV). The National Center for Biotechnology Information (NCBI) database was searched for the amino acid sequences of L7/L12 from <i>Brucella melitensis</i>. In addition, the online softwares, ProtParam and ProtScale, were used to predict the physicochemical properties: NetPhos3.1 and CD-search to predict the phosphorylation sites and conserved domains; SOMPA and SWISS-MODEL to predict the secondary and tertiary structures; the STRING database to analyse the PPIs; and the IEDB, ABCpred, SVMTrip and SYFPEITHI databases to predict the B- and T-cell epitopes. L7/L12 was docked to Toll-like receptor 4 (TLR4), B-cell receptor (BCR), Major histocompatibility complex I-T cell receptor (MHC I-TCR) and MHC II-TCR complexes, respectively, and the binding ability of L7/L12 to the targeted receptors was tested. L7/L12, consisting of 124 amino acids, was determined to be a stable, intracellular, hydrophilic protein containing 6 phosphorylation sites and ribosomal protein-related conserved domains. α-helices accounted for 70.16 %, β-turns for 2.42 %, extended strands for 8.87 % and irregular coils for 18.55 % of the secondary structure. The PPIs indicated that L7/L12 was involved in the constitution of ribosomes and regulating the accuracy of the translation process. Three B-cells, two cytotoxic T lymphocytes and three helper T lymphocyte epitopes were finally screened by comparing multiple databases. L7/L12 binds to TLR4, BCR, MHC I-TCR and MHC II-TCR complexes and forms stable hydrogen bonds, respectively. L7/L12, which governs the translation curate of proteins, possesses several potentially advantageous epitopes, laying a theoretical foundation for designing MEVs.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11648563/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2024-10-10eCollection Date: 2024-01-01DOI: 10.1099/acmi.0.000852.v4
David Midlick, Jason Harhay, Nathan A Summers
{"title":"Carbapenem-resistant Citrobacter amalonaticus and VRE bacteraemia in an immunocompetent patient after a urological Rezum procedure.","authors":"David Midlick, Jason Harhay, Nathan A Summers","doi":"10.1099/acmi.0.000852.v4","DOIUrl":"10.1099/acmi.0.000852.v4","url":null,"abstract":"<p><p>In this report, we discuss the case of a 62-year-old man who presented with gross haematuria, fever, and chills 1 day after undergoing a Rezum procedure and was found to have carbapenem-resistant <i>Citrobacter amalonaticus</i> and vancomycin-resistant <i>Enterococcus faecalis</i> bacteraemia. The patient was treated with daptomycin, eravacycline, and ceftalozane-tazobactam with positive results. We discuss our case and treatment of <i>C. amalonaticus</i> bacteraemia, a pathogen with limited existing literature on its incidence, presentation, and treatment.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11465632/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142402584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2024-10-08eCollection Date: 2024-01-01DOI: 10.1099/acmi.0.000855.v3
Bart Claushuis, Arnoud H de Ru, Peter A van Veelen, Paul J Hensbergen, Jeroen Corver
{"title":"Characterization of the Clostridioides difficile 630Δerm putative Pro-Pro endopeptidase CD1597.","authors":"Bart Claushuis, Arnoud H de Ru, Peter A van Veelen, Paul J Hensbergen, Jeroen Corver","doi":"10.1099/acmi.0.000855.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000855.v3","url":null,"abstract":"<p><p><i>Clostridioides difficile</i> is the leading cause of antibiotic-associated infections worldwide. Within the host, <i>C. difficile</i> can transition from a sessile to a motile state by secreting PPEP-1, which releases the cells from the intestinal epithelium by cleaving adhesion proteins. PPEP-1 belongs to the group of Pro-Pro endopeptidases (PPEPs), which are characterized by their unique ability to cleave proline-proline bonds. Interestingly, another putative member of this group, CD1597, is present in <i>C. difficile</i>. Although it possesses a domain similar to other PPEPs, CD1597 displays several distinct features that suggest a markedly different role for this protein. We investigated the proteolytic activity of CD1597 by testing various potential substrates. In addition, we investigated the effect of the absence of CD1597 by generating an insertional mutant of the <i>cd1597</i> gene. Using the <i>cd1597</i> mutant, we sought to identify phenotypic changes through a series of <i>in vitro</i> experiments and quantitative proteomic analyses. Furthermore, we aimed to study the localization of this protein using a fluorogenic fusion protein. Despite its similarities to PPEP-1, CD1597 did not show proteolytic activity. In addition, the absence of CD1597 caused an increase in various sporulation proteins during the stationary phase, yet we did not observe any alterations in the sporulation frequency of the <i>cd1597</i> mutant. Furthermore, a promoter activity assay indicated a very low expression level of <i>cd1597</i> in vegetative cells, which was independent of the culture medium and growth stage. The low expression was corroborated by our comprehensive proteomic analysis of the whole cell cultures, which failed to identify CD1597. However, an analysis of purified <i>C. difficile</i> spores identified CD1597 as part of the spore proteome. Hence, we predict that the protein is involved in sporulation, although we were unable to define a precise role for CD1597 in <i>C. difficile.</i></p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11460543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2024-10-07eCollection Date: 2024-01-01DOI: 10.1099/acmi.0.000827.v3
German Matias Traglia, Mousami Poudel, Samuel Miño, Blanca Isabel Canteros, G V Minsavage, Anuj Sharma, Erica M Goss, Jeffrey B Jones, Alberto Gochez
{"title":"Xanthomonas citri pv. eucalyptorum strain 4866-2_S43 (formerly X. axonopodis pv. eucalyptorum): the causal agent of bacterial leaf blight on eucalypts recovered in Argentina.","authors":"German Matias Traglia, Mousami Poudel, Samuel Miño, Blanca Isabel Canteros, G V Minsavage, Anuj Sharma, Erica M Goss, Jeffrey B Jones, Alberto Gochez","doi":"10.1099/acmi.0.000827.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000827.v3","url":null,"abstract":"<p><p>We report the draft genome assembly of strain 4866-2_S43 isolated from a eucalyptus lesion in Argentina and what until recently was caused by <i>Xanthomonas citri</i> pv. <i>eucalyptorum</i> (<i>Xce</i>). The genome size is 5 188 607 bp, with a G+C content of 64.66%. Comparative analysis reveals that the closest relative of strain 4866-2_S43 is <i>Xce</i> LPF 602, isolated in Brazil. Comparison of the whole genome sequences revealed an average nucleotide identity (ANI) of 99.96% between the two strains. ANIs were determined between the whole genome sequence of strain 4866-2_S43 and the genomes of all currently validated <i>Xanthomonas</i> spp. These results revealed that strain 4866-2_S43 shared >95% similarity with <i>X. citri</i> pv. <i>citri</i> and <i>X. citri</i> pv. <i>phaseoli</i>, and <95% with <i>X. euvesicatoria</i> pv. <i>alfalfae</i>, <i>X. euvesicatoria</i> pv. <i>perforans</i>, and <i>X. euvesicatoria</i> pathovars <i>euvesicatoria</i> and <i>eucalyptii</i>.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11475742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142485099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2024-10-07eCollection Date: 2024-01-01DOI: 10.1099/acmi.0.000853.v4
Soulandi Djorwé, Abderrahim Malki, Néhémie Nzoyikorera, Joseph Nyandwi, Samuel Privat Zebsoubo, Kawthar Bellamine, Amale Bousfiha
{"title":"Genetic diversity and genomic epidemiology of SARS-CoV-2 during the first 3 years of the pandemic in Morocco: comprehensive sequence analysis, including the unique lineage B.1.528 in Morocco.","authors":"Soulandi Djorwé, Abderrahim Malki, Néhémie Nzoyikorera, Joseph Nyandwi, Samuel Privat Zebsoubo, Kawthar Bellamine, Amale Bousfiha","doi":"10.1099/acmi.0.000853.v4","DOIUrl":"https://doi.org/10.1099/acmi.0.000853.v4","url":null,"abstract":"<p><p>During the 3 years following the emergence of the COVID-19 pandemic, the African continent, like other regions of the world, was substantially impacted by COVID-19. In Morocco, the COVID-19 pandemic has been marked by the emergence and spread of several SARS-CoV-2 variants, leading to a substantial increase in the incidence of infections and deaths. Nevertheless, the comprehensive understanding of the genetic diversity, evolution, and epidemiology of several viral lineages remained limited in Morocco. This study sought to deepen the understanding of the genomic epidemiology of SARS-CoV-2 through a retrospective analysis. The main objective of this study was to analyse the genetic diversity of SARS-CoV-2 and identify distinct lineages, as well as assess their evolution during the pandemic in Morocco, using genomic epidemiology approaches. Furthermore, several key mutations in the functional proteins across different viral lineages were highlighted along with an analysis of the genetic relationships amongst these strains to better understand their evolutionary pathways. A total of 2274 genomic sequences of SARS-CoV-2 isolated in Morocco during the period of 2020 to 2023, were extracted from the GISAID EpiCoV database and subjected to analysis. Lineages and clades were classified according to the nomenclature of GISAID, Nextstrain, and Pangolin. The study was conducted and reported in accordance with STROBE (Strengthening the Reporting of Observational Studies in Epidemiology) guidelines. An exhaustive analysis of 2274 genomic sequences led to the identification of 157 PANGO lineages, including notable lineages such as B.1, B.1.1, B.1.528, and B.1.177, as well as variants such as B.1.1.7, B.1.621, B.1.525, B.1.351, B.1.617.1, B.1.617.2, and its notable sublineages AY.33, AY.72, AY.112, AY.121 that evolved over time before being supplanted by Omicron in December 2021. Among the 2274 sequences analysed, Omicron and its subvariants had a prevalence of 59.5%. The most predominant clades were 21K, 21L, and 22B, which are respectively related phylogenetically to BA.1, BA.2, and BA.5. In June 2022, Morocco rapidly observed a recrudescence of cases of infection, with the emergence and concurrent coexistence of subvariants from clade 22B such as BA.5.2.20, BA.5, BA.5.1, BA.5.2.1, and BF.5, supplanting the subvariants BA.1 (clade display 21K) and BA.2 (clade display 21L), which became marginal. However, XBB (clade 22F) and its progeny such XBB.1.5(23A), XBB.1.16(23B), CH.1.1(23C), XBB.1.9(23D), XBB.2.3(23E), EG.5.1(23F), and XBB.1.5.70(23G) have evolved sporadically. Furthermore, several notable mutations, such as H69del/V70del, G142D, K417N, T478K, E484K, E484A, L452R, F486P, N501Y, Q613H, D614G, and P681H/R, have been identified. Some of these SARS-CoV-2 mutations are known to be involved in increasing transmissibility, virulence, and antibody escape. This study has identified several distinct lineages and mutations involved in the genetic diversity o","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11457919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2024-10-07eCollection Date: 2024-01-01DOI: 10.1099/acmi.0.000754.v3
May Soe Thu, Vorthon Sawaswong, Prangwalai Chanchaem, Pavit Klomkliew, Barry J Campbell, Nattiya Hirankarn, Joanne L Fothergill, Sunchai Payungporn
{"title":"Optimization of a DNA extraction protocol for improving bacterial and fungal classification based on Nanopore sequencing.","authors":"May Soe Thu, Vorthon Sawaswong, Prangwalai Chanchaem, Pavit Klomkliew, Barry J Campbell, Nattiya Hirankarn, Joanne L Fothergill, Sunchai Payungporn","doi":"10.1099/acmi.0.000754.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000754.v3","url":null,"abstract":"<p><p>Ribosomal RNA gene amplicon sequencing is commonly used to evaluate microbiome profiles in health and disease and document the impact of interventional treatments. Nanopore sequencing is attractive since it can provide greater classification at the species level. However, optimized protocols to target marker genes for bacterial and fungal profiling are needed. To achieve an increased taxonomic resolution, we developed extraction and full-length amplicon PCR-based approaches using Nanopore sequencing. Three lysis conditions were applied to a mock microbial community, including known bacterial and fungal species: ZymoBIOMICS lysis buffer (ML) alone, incorporating bead-beating (MLB) or bead-beating plus MetaPolyzyme enzymatic treatment (MLBE). In profiling of bacteria in comparison to reference data, MLB had more statistically different bacterial phyla and genera than the other two conditions. In fungal profiling, MLB had a significant increase of Ascomycota and a decline of Basidiomycota, subsequently failing to detect <i>Malassezia</i> and <i>Cryptococcus</i>. Also, a principal coordinates analysis plot by the Bray-Curtis metric showed a significant difference among groups for bacterial (<i>P=</i>0.033) and fungal (<i>P=</i>0.012) profiles, highlighting the importance of understanding the biases present in pretreatment. Overall, microbial profiling and diversity analysis revealed that ML and MLBE are more similar than MLB for both bacteria and fungi; therefore, using this specific pipeline, bead-beating is not recommended for whole gene amplicon sequencing. However, ML alone was suggested as an optimal approach considering DNA yield, taxonomic classification, reagent cost and hands-on time. This could be an initial proof-of-concept study for simultaneous human bacterial and fungal microbiome studies.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11457918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2024-10-03eCollection Date: 2024-01-01DOI: 10.1099/acmi.0.000838.v3
T T Pattiyakumbura, K G K Malkanthi, W K H Dheerasekara, A Manamperi, M A R V Muthugala
{"title":"Detection of hepatitis B virus genotypes in a group of hepatitis B virus-infected patients in central and northern Sri Lanka.","authors":"T T Pattiyakumbura, K G K Malkanthi, W K H Dheerasekara, A Manamperi, M A R V Muthugala","doi":"10.1099/acmi.0.000838.v3","DOIUrl":"10.1099/acmi.0.000838.v3","url":null,"abstract":"<p><p><b>Introduction.</b> Hepatitis B infection causes a spectrum of clinical diseases varying from asymptomatic infection to severe or fulminant acute hepatitis, chronic liver disease, cirrhosis and hepatocellular carcinoma. Hepatitis B virus (HBV) genotypes appear to influence transmission dynamics, clinical outcomes and responses to antiviral therapy. However, hepatitis B genotyping has been poorly investigated in Sri Lanka. This study intended to determine hepatitis B genotypes in a group of HBV-infected people in central and northern Sri Lanka. <b>Methodology.</b> The study was a laboratory-based descriptive cross-sectional study. Initial detection of HBV DNA in 100 EDTA blood samples was done by using a commercially validated quantitative real-time PCR kit. Hepatitis B genotyping was performed by in-house conventional semi-nested multiplex PCR using genotype-specific primers (for genotypes A-F). The serological profile was determined using a commercially validated ELISA/chemiluminescence immunoassay. The results were evaluated for genotype prevalence, viral load association and hepatitis B e antigen (HBeAg) expression in the study population. <b>Results and conclusion.</b> The study detected that genotype C (<i>n</i>=38) is most prevalent and infections with multiple genotypes (<i>n</i>=52, 52%) were commoner than mono-genotype (<i>n</i>=23, 23%) infections. In total, 25% of patients had no detectable genotype among genotypes A-F. The mean viral load in asymptomatic patients with a single genotype was 3.28 log<sub>10</sub> copies ml<sup>-1</sup> and in multiple genotypes was 4.18 log<sub>10</sub> copies ml<sup>-1</sup> before treatment. Statistical significance was not detected in mean viral loads and HBeAg expression in these two groups. In the future, chronic HBV infection may be effectively treated and managed according to the infected genotype.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11449138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142383173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2024-10-03eCollection Date: 2024-01-01DOI: 10.1099/acmi.0.000796.v3
Athira Cheruvari, Rajagopal Kammara
{"title":"Draft genome sequence of a Lactiplantibacillus pentosus strain isolated from traditionally fermented rice.","authors":"Athira Cheruvari, Rajagopal Kammara","doi":"10.1099/acmi.0.000796.v3","DOIUrl":"10.1099/acmi.0.000796.v3","url":null,"abstract":"<p><p><i>Lactiplantibacillus pentosus</i> is a probiotic bacterium reported to be present in various fermented foods, such as fermented olives, and it significantly influences human health. The present study concerns a lactic acid bacterial strain designated <i>L. pentosus</i> krglsrbmofpi2, isolated from traditional fermented rice, and which has been shown to have an assortment of beneficial attributes. Using Illumina technologies, we have sequenced and investigated the whole genome sequence of <i>L. pentosus</i> krglsrbmofpi2 to understand its functionality and safety. The chromosomal genome was 3.7 Mb in size with 46% GC content and 3192 protein-coding genes. Additional extensive bioinformatics investigations were carried out involving whole genome sequence assembly and annotation.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11449135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142383174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Inducible clindamycin resistance among clinical Gram-positive cocci in a tertiary hospital in Niger Republic.","authors":"Abdourahamane Yacouba, Malika Zeidou Alassoum, Boubacar Marou Soumana, Sahada Moussa Saley, Abdoulaye Ousmane, Harouna Moussa, Saidou Amatagas, Daouda Alhousseini, Mahamadou Doutchi, Salao Chaibou, Mamane Daou, Souleymane Brah, Eric Adehossi, Ahmed Olowo-Okere, Saidou Mamadou","doi":"10.1099/acmi.0.000708.v4","DOIUrl":"10.1099/acmi.0.000708.v4","url":null,"abstract":"<p><p><b>Background</b>. Macrolide-induced resistance to clindamycin is a well-described mechanism leading to treatment failure. Herein, we determined the frequency and associated factors of inducible clindamycin resistance in Gram-positive cocci in a tertiary care hospital. <b>Methods</b>. A cross-sectional descriptive study was carried out between January and December 2022. d-tests were performed as recommended by EUCAST 2021 guidelines on 100 non-duplicate clinical isolates of Gram-positive cocci to determine the prevalence of methicillin resistance and inducible clindamycin resistance among the collected isolates. <b>Results</b>. Of the 100 Gram-positive cocci isolates, 56 (56.0%), 17 (17.0%) and 27 (27.0%) were respectively coagulase-negative staphylococci, <i>Staphylococcus aureus</i> and <i>Streptococcus</i> spp. Among <i>Streptococcus</i> spp., Group D Streptococci (15.0%) were the most isolated. Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) represented nine (53.0%) of the <i>S. aureus</i> isolates. Constitutive (cMLSb) and inducible clindamycin resistance (iMLSb) phenotypes were detected in 36 (36.0%) and 14 (14.0 %) of the isolates, respectively. <i>S. aureus</i> exhibited 38.4% of cMLSb and 13.7% of iMLSb. The result of multivariate analysis showed that age groups, gender, type of samples, provenance, and bacteria, were not significantly associated with Gram-positive cocci iMLSb phenotype. <b>Conclusion</b>. The study reported for the first time a high prevalence of inducible resistance of Gram-positive cocci strains to clindamycin in Niger Republic. This suggests the urgent need for the implementation of regular screening of these isolates and the wise use of clindamycin in clinical practice.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11649245/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}