Access microbiology最新文献

{"title":"Identification of causative fungus from sterile abscess using metagenomics followed by in situ hybridization.","authors":"Hiroya Oki, Ryotaro Niwa, Somboonthum Pranee, Daisuke Motooka, Yoshiyuki Onda, Jun Nakata, Hiroko Nakajima, Yoshihiro Oka, Haruo Sugiyama, Yuka Yoshii, Naoyuki Anzai, Shota Nakamura, Tetsuya Iida","doi":"10.1099/acmi.0.000779.v3","DOIUrl":"10.1099/acmi.0.000779.v3","url":null,"abstract":"<p><p><b>Introduction.</b> Invasive fungal infections require early diagnosis for treatment. Microscopic observation of biopsy and blood culture is the gold standard for the identification of the causative fungus, but it is difficult to identify the causative pathogen by a sterile abscess biopsy. <b>Case Presentation.</b> We present a case report of breakthrough invasive trichosporonosis in a 65-year-old Japanese male with acute myeloid leukaemia receiving antifungal prophylaxis. Blood cultures showed no fungal growth, and a liver biopsy and a removed spleen with abscess showed fragmented fungi, but no fungal identification was possible. This report demonstrates that retrospective analyses were able to identify the causative fungus. <b>Conclusion.</b> We narrowed down the candidate fungi by deep sequencing of the ITS1 region of fungal genome and confirmed that the fungus observed in the specimen was <i>Trichosporon asahii</i> by <i>in situ</i> hybridization using a DNA probe targeting 26S rRNA.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11325578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141989879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fatal Clostridium septicum gas gangrene complicating ECMO: case report and review of literature.","authors":"Adrien Turban, Vincent Joussellin, Caroline Piau, Vincent Cattoir, Yoann Launey, Gabriel Eustache","doi":"10.1099/acmi.0.000825.v3","DOIUrl":"10.1099/acmi.0.000825.v3","url":null,"abstract":"<p><p><i>Clostridium septicum</i> gas gangrene is a severe and deadly infection caused by an anaerobic, spore-forming, Gram-positive bacillus. As previously described, two entities are observed: traumatic and spontaneous (or non-traumatic) forms. In this report, we aim to describe the case of a fulminant and ultimately fatal <i>C. septicum</i> myonecrosis occurring in a patient who was first admitted for refractory cardiac arrest and placed on veino-arterial extracorporeal membrane oxygenation (ECMO). Building upon prior studies that have documented cases of spontaneous gas gangrene caused by <i>C. septicum</i>, we provide an updated compilation, focusing on microbiological characteristics of <i>C. septicum</i>, along with the diagnostic and therapeutic challenges associated with spontaneous gas gangrene. Additionally, the specific clinical situation of our case illustrates the seriousness of this infectious complication that combined both spontaneous and traumatic gas gangrene risk factors. We thus, discuss the antibiotic coverage prior to the initiation of ECMO procedure.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11299951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141895128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifocal tuberculosis revealed by a sternal swelling in an immunocompetent child.","authors":"Ghizlane Chehrastane, Elmostafa Benaissa, Abdelilah Radi, Amal El Hassani, Mostafa Elouennass","doi":"10.1099/acmi.0.000795.v3","DOIUrl":"10.1099/acmi.0.000795.v3","url":null,"abstract":"<p><p>Tuberculosis (TB) is one of the most common pathogens of bacterial lung infections, especially in underdeveloped nations like Morocco, where the incidence of TB was 97 cases per 100 000 persons in 2019. Thanks to its national TB prevention and control plan, Morocco was able to achieve remarkable progress in the management of TB with an 80% reduction in the total number of patients diagnosed with TB between 1980 and 2018. The national plan also allowed us to reach and maintain a therapeutic rate above 86% since 2002. Sternal TB is a rare clinical condition accounting for 1% of all musculoskeletal TB cases. Due to its rarity and the lack of awareness of clinical presentations, the diagnosis of sternal TB can be quite complex. We describe the case of a 14-year-old Moroccan patient consulting in the Military Hospital Mohammed V-Rabat with central chest pain for 4 months which was not associated with breathing, physical exercise or eating. The patient also had a history of asthenia, fever and weight loss. A computed tomography scan of the chest showed a destructive lesion of the sternum. Afterward, a chirurgical biopsy was performed and enabled to confirm the microbiological diagnosis of TB with the realization of the real-time PCR. The antitubercular therapy was given to the patient who had complete resolution of symptoms. This condition should be included in the differential diagnosis of chronic chest pain that mimics costochondritis particularly in patients from endemic areas.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11299650/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141895129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acanthamoeba castellanii alone is not a growth promoter for Hordeum vulgare.","authors":"Julia Sacharow, Stefan Ratering, Bellinda Schneider, Alessandra Österreicher Cunha-Dupont, Sylvia Schnell","doi":"10.1099/acmi.0.000761.v3","DOIUrl":"10.1099/acmi.0.000761.v3","url":null,"abstract":"<p><p>Protists are important key players in the microbial loop and influence their environment by grazing, which leads to the return of nutrients into the soil and reduces pathogen pressure on plants. Specifically, protists on and around plant roots are important for plants' development and growth. For this study, the fourth most important crop in the world, <i>Hordeum vulgare</i>, was selected. Seeds of <i>H. vulgare</i> were inoculated with <i>Acanthamoeba castellanii</i> alone or with additional soil bacteria at the beginning and during the experiment. The germination of the seeds and the growth of the plants in pouches were monitored over 3 weeks. No differences were found in leaf growth, root growth, root and leaf nitrogen content or ammonia content of the liquid from the pouches. In contrast, the relative increase in root and leaf dry weight showed a small difference compared to the controls. The results of this experiment demonstrated that seed inoculation with <i>A. castellanii</i> alone or with additional unidentified soil bacteria did not have a major effect on the growth and development of barley. Nevertheless, small changes in plant development were detected, indicating that <i>A. castellanii</i> should be considered for further investigation of co-inoculations with plant growth-promoting bacteria and additional nutrients.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11293846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141891485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration and characterization of a newly isolated bacterium, Enterobacter quasihormaechei strain BDIFST24001, capable of producing rhamnolipid biosurfactant for oil remediation.","authors":"Afsana Habib Jui, Mohammad Nazrul Islam Bhuiyan, Banasree Bhowmik, Nazia Khatun, Abhijit Chowdhury, Riyadh Hossen Bhuiyan, Md Alamgir Kabir, Md Mamunur Rashid, Md Habibur Rahman Bhuiyan, Sadia Afrin","doi":"10.1099/acmi.0.000830.v4","DOIUrl":"10.1099/acmi.0.000830.v4","url":null,"abstract":"<p><p>Biosurfactants are naturally occurring compounds synthesized by micro-organisms that increasingly attract attention due to both their living area and application in various industries. In this study, we explore and characterize a novel bacterium, <i>Enterobacter quasihormaechei</i> strain BDIFST24001, isolated for its ability to produce rhamnolipid biosurfactants, with the aim of facilitating oil remediation processes. The isolation of this bacterium was carried out using Luria-Bertani (LB) broth media from environmental samples collected from oil-contaminated sites in Dhaka City. Screening tests, including the oil spreading method and drop collapse assay, were conducted to identify potential biosurfactant-producing strains, leading to the selection of <i>E. quasihormaechei</i> strain BDIFST24001 based on its favourable performance. Subsequent molecular identification revealed a high similarity of the strain's 16S rRNA gene to <i>E. quasihormaechei</i>, which was corroborated through phylogenetic analysis. Further analysis of the biosurfactant produced by this strain indicated its rhamnolipid nature, as confirmed by FT-IR spectroscopy. The rhamnolipids exhibited promising surface-active properties, including a significant reduction in surface tension and emulsification activity, as evidenced by surface tension measurements and emulsification index assays. Optimization studies revealed that the optimal conditions for rhamnolipid production by <i>E. quasihormaechei</i> strain BDIFST24001 were a temperature of 37 °C, pH 10.0 and salinity of 4 %. The rhamnolipids produced by this strain demonstrated effective oil remediation capabilities, as observed through controlled experiments using petrol oil. The rhamnolipids effectively reduced the surface tension of the oil-water interface, facilitating the dispersion and emulsification of the oil phase in water. Overall, our findings highlight the potential of <i>E. quasihormaechei</i> strain BDIFST24001 as a promising candidate for biosurfactant-mediated oil spill cleanup and environmental remediation efforts.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11293832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141891486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: Enhancement of growth media for extreme iron limitation in Escherichia coli.","authors":"James W Southwell, Keith S Wilson, Gavin H Thomas, Anne-Kathrin Duhme-Klair","doi":"10.1099/acmi.0.000887","DOIUrl":"https://doi.org/10.1099/acmi.0.000887","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1099/acmi.0.000735.v4.].</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 8","pages":"000887"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11652849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A study on viruses and bacteria with particular interest on Mycoplasma pneumoniae in children with exacerbation of asthma from a tertiary care hospital in Sri Lanka.","authors":"Lakmini Inoka Wijesooriya, Victoria Chalker, Priyantha Perera, N P Sunil-Chandra","doi":"10.1099/acmi.0.000778.v5","DOIUrl":"10.1099/acmi.0.000778.v5","url":null,"abstract":"<p><p>Asthma is a significant public health concern, particularly in children with severe symptoms. Exacerbation of asthma (EOA) is life-threatening, and respiratory infections (RIs) play a crucial role. Though viruses play a significant role in EOA, patients are empirically treated with antibiotics, contributing to antibiotic resistance development. Although there are widely reported associations of EOA with viral or <i>Mycoplasma pneumoniae</i> infections, there are no published data for Sri Lanka. The present study aimed to identify the association of common respiratory viruses, typical respiratory bacterial pathogens and <i>M. pneumoniae</i> in children with EOA and relate them with the compatibility of antimicrobial use. A case-control study was conducted in the paediatric unit of North Colombo Teaching Hospital, Sri Lanka, involving two groups of children between 5 and 15 years of age. Group 1 is children with EOA and Group 2 is children with stable asthma (SA). Each group consisted of 100 children. Sputum/throat swabs were tested for common respiratory viruses using virus-specific fluorescein isothiocyanate-labelled monoclonal antibodies (MAbs), bacteria by routine culture, and <i>M. pneumoniae</i> by real-time polymerase chain reaction. Macrolide resistance in <i>M. pneumoniae</i> was detected using conventional PCR and sequencing specific genetic mutations in the 23S rRNA gene. <i>M. pneumoniae</i> was genotyped using nested multilocus sequence typing, which targeted eight housekeeping genes (<i>ppa</i>, <i>pgm</i>, <i>gyrB</i>, <i>gmk</i>, <i>glyA</i>, <i>atpA</i>, <i>arcC</i> and <i>adk</i>). There was no significant difference in age, gender, demographic or geographical location between the two groups. In children with EOA, antibiotics were used in 66 % (66/100) and macrolides in 42 % (42/100). Samples comprised 78 % (78/100) sputum and 22 % (22/100) throat swabs. Adenovirus was the most common virus identified, and it was significantly higher in children with EOA compared to those with SA. Still, the two groups had no significant difference in typical bacteria findings. <i>M. pneumoniae</i> was detected in one patient with EOA, but none was detected in the SA group. The <i>M. pneumoniae</i> was macrolide-sensitive and ST14 by multilocus sequence typing. This study showed that the empiric use of antibiotics in children with asthma might be better targeted with prior pathogen screening to inform appropriate treatment to minimize antibiotic resistance.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11288328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141857543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic and genotypic characterization of extended-spectrum beta-lactamase-producing Enterobacteriaceae in a Moroccan hospital.","authors":"Yassine Eddair, Elmehdi Belouad, Elmostafa Benaissa, Tilila Abassor, Fatna Bsaibiss, Adil Maleb, Mostafa Elouennass","doi":"10.1099/acmi.0.000822.v3","DOIUrl":"10.1099/acmi.0.000822.v3","url":null,"abstract":"<p><p>Extended-spectrum beta-lactamase-producing <i>Enterobacteriaceae</i> (ESBL-E) is a major public health problem in hospitals and in the community. The objective of this work was to describe the epidemiology of ESBL-E<i>,</i> to study their resistance profile and to determine the genes encoding the ESBL phenotype. This is a retrospective study conducted in the bacteriology laboratory of the Mohamed V Military Training Hospital in Rabat, and covering all isolates of <i>Enterobacteriaceae</i> from 1 January 2018 to 31 December 2020. The molecular study of ESBL genes involved a representative sample of all ESBL isolates. The overall prevalence of ESBLs in isolated <i>Enterobacteriaceae</i> (1402/10268) is 13.65 %. The urinary tract was the main site of isolation of ESBL (61 %). The bacterial species most concerned are <i>Escherichia coli</i> (41.9 %), <i>Klebsiella pneumoniae</i> (42.2 %) and <i>Enterobacter cloacae</i> (11.9 %). The study of antibiotic susceptibility showed a resistant profile marked mainly by 100 % resistance to first generation cephalosporins (1GC) and third generation cephalosporins (3GC), 55 % to piperacillin-tazobactam, 16 % to imipenem, and 87 % to fluoroquinolones. Molecular typing of ESBL strains showed a prevalence of CTX-M (95 %), SHV (50 %) and TEM (56 %). The CTX-M-1 and the CTX-M-9 groups were the most common (96.19 % and 7.62 % respectively), and CTX-M15 was found in 78.10 % of CTX-M-1 ESBL positive isolates. Most strains had more than two coexisting resistance genes. The prevalence rate of ESBL-E is critical, and preventive action at different levels (prescriber, biologist, hospital, patient, etc.) are necessary in order to limit their spread and to manage a better therapeutic strategy.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11282464/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141790761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of chemotherapeutic agents on natural transformation frequency in Acinetobacter baylyi","authors":"Macaulay Winter, M. Vos, A. Buckling, Pål J. Johnsen, Klaus Harms","doi":"10.1099/acmi.0.000733.v4","DOIUrl":"https://doi.org/10.1099/acmi.0.000733.v4","url":null,"abstract":"Natural transformation is the ability of a bacterial cell to take up extracellular DNA which is subsequently available for recombination into the chromosome (or maintenance as an extrachromosomal element). Like other mechanisms of horizontal gene transfer, natural transformation is a significant driver for the dissemination of antimicrobial resistance. Recent studies have shown that many pharmaceutical compounds such as antidepressants and anti-inflammatory drugs can upregulate transformation frequency in the model species Acinetobacter baylyi. Chemotherapeutic compounds have been shown to increase the abundance of antimicrobial resistance genes and increase colonization rates of potentially pathogenic bacteria in patient gastrointestinal tracts, indicating an increased risk of infection and providing a pool of pathogenicity or resistance genes for transformable commensal bacteria. We here test for the effect of six cancer chemotherapeutic compounds on A. baylyi natural transformation frequency, finding two compounds, docetaxel and daunorubicin, to significantly decrease transformation frequency, and daunorubicin to also decrease growth rate significantly. Enhancing our understanding of the effect of chemotherapeutic compounds on the frequency of natural transformation could aid in preventing the horizontal spread of antimicrobial resistance genes.","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"26 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141659821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple novel caliciviruses identified from stoats (Mustela erminea) in the United Kingdom.","authors":"Joseph Hinds, Ternenge Apaa, Rhys H Parry, Amy J Withers, Laura MacKenzie, Ceri Staley, Joshua Morrison, Malcolm Bennett, Samantha Bremner-Harrison, Elizabeth A Chadwick, Frank Hailer, Stephen W R Harrison, Xavier Lambin, Mathew Loose, Fiona Mathews, Rachael Tarlinton, Adam Blanchard","doi":"10.1099/acmi.0.000813.v4","DOIUrl":"10.1099/acmi.0.000813.v4","url":null,"abstract":"<p><p>The <i>Caliciviridae</i> family<i>,</i> comprising positive-sense RNA viruses, is characterised by its non-enveloped, small virions, broad host range, and notable tendency for host switching. These viruses are primarily associated with gastroenteric disease, though they can lead to haemorrhagic or respiratory infections. Our study employed a metagenomics analysis of faecal samples from stoats (<i>Mustela erminea</i>), identifying two novel calicivirus species, named stoat vesivirus and stoat valovirus. Stoat vesivirus was identified in three samples (ST008, ST006, ST004), exhibiting a genome wide nucleotide identity of approximately 92 %. The complete coding sequences of these samples were 8471 (ST004) and 8322 (ST006) nucleotides in length, respectively. Each comprised three open reading frames (ORF), closely resembling the <i>Vesivirus</i> mink calicivirus (China/2/2016), with 70-72 % similarity in ORF1, 61-62 % in ORF2 and 71 % in ORF3. Phylogenetic analysis robustly supported stoat vesivirus as belonging within the <i>Vesivirus</i> genus. The second calivicirus (stoat valovirus), detected solely in sample ST008, was 6527 nucleotides in length and with complete coding sequences present. It shared highest similarity with St-Valérien swine virus and marmot norovirus HT16, showing 39.5 and 38.8 % protein identity with ORF1 and 43.3 and 42.9 % for VP1. Stoat valovirus is borderline for meeting the ICTV criteria for a new genus, demonstrating 60 % divergence in ORF1 compared to the other valovirus', however it clusters basally within the <i>Valovirus</i> genus, supporting leaving it included in this genus.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"6 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11316584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141918502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}