Identification of Mammaliicoccus fleurettii as the source of a methicillin-resistant gene in a First Nation reserve lake in Manitoba, Canada.

Access microbiology Pub Date : 2024-11-22 eCollection Date: 2024-01-01 DOI:10.1099/acmi.0.000861.v3

Sabrin Bashar, Rakesh Patidar, Alvan Wai, Dawn White, George R Golding, Annemieke Farenhorst, Ayush Kumar

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Abstract

Our study aimed to identify the bacterial source of a previously detected mobile antibiotic-resistant gene, mecA, found in a lake that serves as a source to a water treatment plant operated by a First Nation reserve. Three methicillin-resistant presumptive Staphylococcus spp. isolated from the sample using selective media were verified as mecA positive by PCR. MALDI-TOF and whole-genome sequencing of each isolate confirmed that all three were Mammaliicoccus fleurettii. Antibiotic-resistant gene analysis of the assembled genomes predicted mecA with 99.7% sequence identity, and phylogenetic analysis grouped our three mecA genes with the mecA allele from a methicillin-resistant strain of Staphylococcus aureus. Identifying microbial species known to harbour mobile antibiotic-resistant elements can provide greater depth of information about drinking water, an especially essential need in First Nation reserves where water quality too frequently is poor.

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加拿大马尼托巴省第一民族保护区湖泊中甲氧西林耐药基因来源的鉴定。

我们的研究旨在确定先前检测到的移动抗生素耐药基因mecA的细菌来源，该基因在一个湖泊中被发现，该湖泊是由第一民族保护区运营的水处理厂的来源。用选择性培养基从样品中分离出3株疑似耐甲氧西林葡萄球菌，经PCR验证为mecA阳性。每个分离物的MALDI-TOF和全基因组测序证实这三个分离物都是fleurettii哺乳球菌。对组装的基因组进行耐药基因分析，预测mecA序列的同源性为99.7%，系统发育分析将我们的3个mecA基因与耐甲氧西林金黄色葡萄球菌菌株的mecA等位基因进行了分组。识别已知含有流动抗生素耐药元素的微生物物种可以提供有关饮用水的更深入的信息，这对于水质经常很差的第一民族保护区来说是一项特别重要的需求。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Access microbiology

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2.00

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