Genome sequences of pathogenic and non-pathogenic Pantoea ananatis strains in maize (Zea mays L.).

Access microbiology Pub Date : 2025-02-14 eCollection Date: 2025-01-01 DOI:10.1099/acmi.0.000709.v3
Izabela Moura Duin, Viviane Yumi Baba, Katherine M D'Amico-Willman, Fernanda Neves Paduan, Vanessa Hitomi Sugahara Rodrigues, Jose C Huguet-Tapia, Jeffrey Bryant Jones, Marcelo G Canteri, Rui Pereira Leite Júnior, Maria Isabel Balbi-Peña
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Abstract

We performed genome sequencing and comparative analysis of Pantoea ananatis strains isolated from corn leaves expressing typical bacterial leaf streak (BLS) and maize white spot (MWS) symptoms to confirm bacterial identity and to understand the relationship among these strains and P. ananatis strains isolated from different plant hosts in Brazil. In pathogenicity tests, strains 4.2 and 13.3 isolated from symptomatic BLS leaves were non-pathogenic on corn. In contrast, strain B13 isolated from MWS-diseased leaf tissue caused symptoms typical of MWS. Our comparative analysis revealed that all three strains are very genetically similar. The G+C (%) content of strains 4.2 and 13.3 was 53.5%, while the B13 content was 53.7%. Average nucleotide identity (ANI) analysis showed that strains B13 and 13.3, B13 and 4.2, and 4.2 and 13.3 shared ANIs of 99.17%, 99.15% and 99.99%, respectively. Strains 13.3, B13, and 4.2 shared ~99% ANI with P. ananatis type strain LMG 2665. To the best of our knowledge, these are the first genome sequences of P. ananatis strains isolated from corn in Brazil.

玉米(Zea mays L.)致病性和非致病性泛菌基因组序列研究
我们对从玉米叶片中分离出的表达典型细菌性叶片条纹(BLS)和玉米白斑(MWS)症状的Pantoea ananatis菌株进行了基因组测序和比较分析,以确认细菌的身份,并了解这些菌株与从巴西不同植物宿主分离的Pantoea ananatis菌株之间的关系。菌株4.2和菌株13.3对玉米无致病性。相比之下,从MWS病叶组织中分离的菌株B13引起了MWS的典型症状。我们的比较分析显示,这三种菌株在基因上非常相似。菌株4.2和菌株13.3的G+C(%)含量为53.5%,B13含量为53.7%。平均核苷酸同源性(ANI)分析显示,菌株B13与13.3、B13与4.2、4.2与13.3的同源性分别为99.17%、99.15%和99.99%。菌株13.3、B13和4.2与P. ananatis型菌株LMG 2665具有~99%的ANI。据我们所知,这些是从巴西玉米中分离出的第一个ananatis菌株的基因组序列。
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