Multi-metal-resistant Staphylococcus warneri strain TWSL_1: revealing heavy metal-resistant genomic features by whole-genome sequencing and analysis.

Access microbiology Pub Date : 2025-05-27 eCollection Date: 2025-01-01 DOI:10.1099/acmi.0.000954.v5
Dilani Chathurika Dissanayake, Naduviladath Vishvanath Chandrasekharan, Champika Dilrukshi Wijayarathna
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Abstract

This study explores the genomic basis of heavy metal resistance in Staphylococcus warneri strain TWSL_1, isolated from industrial textile effluent. The strain exhibited strong resistance to Cd²+, Pb²+ and Cu²+, with MICs of 50, 1,200 and 75 mg l-1, respectively. Whole-genome sequencing revealed a 2.66 Mb genome with 2,567 CDSs and a 99.81% average nucleotide identity to S. warneri WS479. Comparative genomic analysis at the genus level revealed that S. warneri strain TWSL_1 possesses a unique and expanded repertoire of heavy metal resistance genes, including the cadmium efflux system accessory protein and cadmium resistance protein, which are absent in pathogenic Staphylococcus sp. used for the comparison. Phylogenetic analysis confirmed its classification within S. warneri, with strong bootstrap support (100). Functional annotation highlighted metabolic versatility and stress response capabilities, supporting its adaptation to metal-rich environments. S. warneri TWSL_1 demonstrated high Pb²+ removal efficiency, reducing concentrations by over 70%. These findings highlight S. warneri TWSL_1 as a promising candidate for heavy metal bioremediation with potential applications in environmental detoxification and monitoring strategies.

耐多金属瓦纳利葡萄球菌菌株TWSL_1:通过全基因组测序和分析揭示耐重金属基因组特征
本研究探讨了从工业纺织废水中分离的瓦纳里葡萄球菌TWSL_1对重金属抗性的基因组基础。菌株对Cd²+、Pb²+和Cu²+具有较强的抗性,mic分别为50、1200和75 mg l-1。全基因组测序结果显示,该基因组全长2.66 Mb,包含2,567个cds,平均核苷酸与S. warneri WS479同源性为99.81%。在属水平上的比较基因组分析表明,warneri菌株TWSL_1具有独特和扩展的重金属抗性基因库,包括镉外排系统辅助蛋白和镉抗性蛋白,这是用于比较的致病性葡萄球菌所缺乏的。系统发育分析证实其属于S. warneri,具有很强的bootstrap支持(100)。功能注释强调了代谢的多功能性和应激反应能力,支持其适应富金属环境。S. warneri TWSL_1表现出较高的Pb²+去除率,浓度降低70%以上。这些发现突出了S. warneri TWSL_1作为重金属生物修复的潜在候选者,在环境解毒和监测策略方面具有潜在的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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