Draft genome sequences of four potential new species of the genus Bradyrhizobium isolated from root nodules of native legumes in Costa Rican forests.

Access microbiology Pub Date : 2025-05-16 eCollection Date: 2025-01-01 DOI:10.1099/acmi.0.000990.v3

Rachelle Fernández-Vargas, Sara Jiménez-Alpízar, Valeria Leandro-Arce, Bradd Mendoza-Guido, Keilor Rojas-Jimenez

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Abstract

Here, we report the draft genome sequences of four Bradyrhizobium spp. isolates obtained from root nodules of the native legumes Pentaclethra macroloba, Chamaecrista nictitans, Erythrina fusca and Zygia engelsingii in tropical forests of Costa Rica. Genomes ranged from 8.6 to 9.8 Mb with GC contents between 62.8% and 63.8%. Phylogenomic analysis, along with average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) comparisons, confirmed that these isolates represent potential new species. ANI values ranged from 88.3% to 90.3%, and dDDH values from 28.8% to 41.8%, compared with their closest Bradyrhizobium species. Functional annotation revealed some genes related to nitrogen fixation (nifA, nifB, nifH) and nodulation capacity (nodB, nodC, nodJ). These results provide insights into the diversity and symbiotic capabilities of Bradyrhizobium in tropical ecosystems.

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从哥斯达黎加森林原生豆科植物根瘤中分离的四个潜在新种的缓生根瘤菌属基因组序列草图。

在这里，我们报告了从哥斯达黎加热带森林的原生豆科植物Pentaclethra macroloba、Chamaecrista nictitans、Erythrina fusca和Zygia engelsingii的根瘤中分离得到的四种慢生根瘤菌的基因组序列草图。基因组大小在8.6 ~ 9.8 Mb之间，GC含量在62.8% ~ 63.8%之间。系统基因组分析以及平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）比较证实，这些分离物代表了潜在的新物种。ANI值为88.3% ~ 90.3%，dDDH值为28.8% ~ 41.8%。功能注释显示了一些与固氮有关的基因（nifA, nifB, nifH）和结瘤能力（nodB, nodC, nodJ）。这些结果提供了对热带生态系统中缓生根瘤菌的多样性和共生能力的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Access microbiology

CiteScore

2.00

自引率

0.00%

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