Bio-medical materials and engineering最新文献

{"title":"Design strategies for titanium surfaces to adsorb and release drugs: An in vitro study.","authors":"Pablo Yael Carrazco Ávila, Juan Ignacio Rosales Leal, Miguel Ángel Rodríguez Valverde, María Encarnación Morales Hernandez","doi":"10.1177/09592989251346461","DOIUrl":"https://doi.org/10.1177/09592989251346461","url":null,"abstract":"<p><strong>Background: </strong>During the early postoperative period, it is important that the patient recovers without pain and inflammation, while preserving their quality of life. In this sense, coated titanium surfaces have been designed to release anti-inflammatory and analgesic drugs during the first postoperative hours.</p><p><strong>Objective: </strong>Evaluate the adsorption capacity and release profile of different bioactive titanium surfaces treated with three different drugs: acetaminophen, doxepin and ibuprofen.</p><p><strong>Methods: </strong>Four bioactive surfaces (polished, oxidized, hydroxyapatite precipitate, and polyvinyl alcohol coating surfaces) were physiochemically treated and analyzed.</p><p><strong>Results: </strong>Hydroxyapatite coatings were the roughest, while PVA coating was the softest. Acetaminophen was the only drug detected on all the surfaces. In contrast, higher drug doses were loaded into the PVA coatings, showing a satisfactory release profile.</p><p><strong>Conclusion: </strong>The results suggest that a rougher or ionically charged surface does not guarantee drug adsorption. In contrast, the use of a transport vehicle such as a polyvinyl coating ensures the release of the drug, initiating its therapeutic effect within the first minutes, and maintained for a period of between 120 and 180 min.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"9592989251346461"},"PeriodicalIF":1.0,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BMP-2 enhanced new bone formation of calcium phosphate-based composites in mice.","authors":"Liuxing He, Xue Wang, Nana Xiong, Zhihong Dong, Lijia Cheng","doi":"10.1177/09592989251346468","DOIUrl":"https://doi.org/10.1177/09592989251346468","url":null,"abstract":"<p><strong>Background: </strong>Autologous bone grafting, particularly using iliac crest bone grafts, historically been considered the gold standard for bone grafting. However, its limited availability and secondary surgical trauma associated with the procedure have restricted its widespread application.</p><p><strong>Objective: </strong>To investigate the osteogenic potential of calcium phosphate-based composites with or without the addition of bone morphogenetic protein-2 (BMP-2).</p><p><strong>Methods: </strong>The BMP-2/calcium phosphate (CaP)/temperature-sensitive hydrogel (BCT) composite was synthesized, while the CaP/temperature-sensitive hydrogel (CT) composite was prepared as control group. The surface morphology of the composites was observed using scanning electron microscope (SEM). Subsequently, the composites were co-cultured with mouse bone marrow mesenchymal stem cells (BMSCs), and the cell proliferation, alkaline phosphatase (ALP) activity and extracellular matrix (ECM) mineralization were assessed. The composites were injected into the muscle of mice, and the samples underwent hematoxylin and eosin (HE), Masson-trichrome, and safranin and fast green staining. Immunohistochemistry for BMP-2 and type I collagen (ColI) was performed at weeks 8 and 12.</p><p><strong>Results: </strong>There was no significant difference in cell proliferation between the BCT and CT groups. However, the relative ALP activity and ECM mineralization were significantly higher in BCT group compared to the CT group (<i>P</i> < 0.05). BMP-2 accelerated the osteoinduction process and promoted the formation of more new bone tissue and bone marrow in group BCT. The number of osteocytes and the collagen area ratio were significantly higher in group BCT than that in group CT (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>BMP-2 and CaP synergistically accelerated the initiation of osteoinduction, and promoted increased bone formation. The temperature-sensitive hydrogel made the material injectable, expanding its range of applications. The BCT composite shows promise as an artificial bone material.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"9592989251346468"},"PeriodicalIF":1.0,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrically stimulated drug release using conductive GelMA/k-carrageenan/rGO blended hydrogel for enhanced biomedical applications.","authors":"Jong Min Lee","doi":"10.1177/09592989251343077","DOIUrl":"https://doi.org/10.1177/09592989251343077","url":null,"abstract":"<p><p>BackgroundHydrogels are hydrophilic polymers with high water content and a porous structure, making them suitable for incorporating water-soluble drugs and functioning as drug delivery systems. Their structural similarity to living tissues renders them valuable for applications in tissue engineering, pharmaceuticals, and medical treatments.ObjectiveThis study aimed to develop a blended hydrogel with improved mechanical strength and biocompatibility, and to enhance its drug release capabilities through electrical stimulation.MethodA conductive hydrogel was synthesized by blending gelatin methacrylate (GelMA), kappa carrageenan (k-carrageenan), and reduced graphene oxide (rGO). The hydrogel's physical integrity, biocompatibility, and drug release performance under electrical stimulation were evaluated.ResultsThe GelMA/k-carrageenan/rGO hydrogel retained its structural stability, demonstrated excellent biocompatibility, and effectively released drugs in response to electrical stimulation.ConclusionThe developed conductive hydrogel presents strong potential for advanced drug delivery systems utilizing electrical stimulation, with promising implications across biomedical and pharmaceutical fields.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"9592989251343077"},"PeriodicalIF":1.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing biomaterial application strategies for neurorestoration after ischemic stroke injury: An emerging treatment option.","authors":"Asmaa' Mohd Satar, Farah Amna Othman, Suat Cheng Tan","doi":"10.1177/09592989241306679","DOIUrl":"10.1177/09592989241306679","url":null,"abstract":"<p><p>BackgroundIschemic stroke is a medical condition caused by occlusion of blood vessels in brain, resulting in disruption of blood flow to the brain and triggering irreversible damage to the neuronal cells. While stem cells transplantation has been proposed as a potential alternative therapym for ischemic stroke, its effectiveness is limited due to low cell survival rate and potential side effects following transplantation. To overcome these challenges and enhance therapeutics efficacy, researchers have focused on developing various biomaterials to create a sustainable cellular microenvironment or to modify the properties of donor stem cell which could optimize their reparative functions in injured brain tissues.ObjectiveThis review aims to explore and discuss the different types of biomaterials that have been applied in the treatment of ischemic stroke, shedding light on their potentials as promising therapeutics options for this debilitating condition.MethodsLiterature search was performed to identify publications studying the potential of three biomaterials namely: nanobioparticles, hydrogels and extracellular vesicles for ischemic stroke therapy in vitro, in vivo or in clinical using four databases, namely: PubMed, ScienceDirect, Web of Science and Scopus.Results and discussionThe major benefits obtained from the application of nanobioparticles for ischemic stroke therapy included as the nanocarrier for drug/cell delivery, cell tracking, real time imaging, promote cell proliferation, while hydrogels provided scaffold support and conferred neuroprotection to stem cells, as well as provided neurotropic effects and controlled drug release for localized treatment. Lastly the extracellular vesicles were identified as a cell-free treatment strategy in promoting angiogenesis, neuronal differentiation and neurogenesis for ischemic stroke treatment.ConclusionBiomaterial-based therapies have their own potentials and further clinical investigations are strongly recommended to translate the therapies into more conscientious evidence-based therapy for clinical application.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"151-162"},"PeriodicalIF":1.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced detection of Mycobacterium Tuberculosis using nanogold-based silver staining enhancement.","authors":"Lili Quan, Qianrong Yang, Zhu Jin, Rong Li, Han Xiao, Lu Wang","doi":"10.1177/09592989241308792","DOIUrl":"10.1177/09592989241308792","url":null,"abstract":"<p><p>BackgroundTuberculosis (TB) is a global health challenge from a single infectious agent, Mycobacterium tuberculosis (MTB), and it demands improved diagnostics and therapies.ObjectiveThis work explored a novel method for detecting MTB by combining nanogold labeling (NGL) technology with silver staining to enhance sensitivity and specificity.MethodsNanogold particles (NGPs) were characterized using ultraviolet absorption spectroscopy (UVAS), and their morphology was observed via transmission electron microscopy (TEM). The silver staining enhancement (SSE) system was optimized for a reaction time of 11 min. Fifty drug-resistant tuberculosis (DRT) patients were randomly assigned to a control (Ctrl) group receiving conventional nursing and an experimental (Exp) group treated with continuous nursing intervention (CNI). Quality of Life Instrument for Tuberculosis Patients (QLI-TB) scores were compared over 6 months.ResultsUnmarked NGPs were evenly distributed, while labeled NGPs maintained complete morphology with a gray halo. The detection limit was established at 0.582, reaching as low as 1 pmol/L. For sputum specimens, detection rates were 38.7% for culture, 41.94% for PCR, and 43.54% for nanogold SSE, with no significant differences (<i>P </i>> 0.05). However, patients in the Exp group exhibited significant improvements in physical, psychological, and social functions, as well as the tuberculosis-specific module (TSM) compared to the Ctrl group (<i>P </i>< 0.05).ConclusionsWe demonstrated an innovative method for detecting MTB, demonstrating promising results through method optimization and analysis.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"163-173"},"PeriodicalIF":1.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomechanical properties of regenerated digital flexor tendon in immature newt following complete transection.","authors":"Yu Masuda-Otsuka, Tomoka Kamiya, Daisuke Suzuki, Toshinori Hayashi, Jeonghyun Kim, Takeo Matsumoto, Eijiro Maeda","doi":"10.1177/09592989251324288","DOIUrl":"https://doi.org/10.1177/09592989251324288","url":null,"abstract":"<p><p>BackgroundAdult newt (9-month-old) has become an emerging research model to study complete regeneration of injured adult tendon. If younger newts exhibit tendon regeneration similar to adult ones, they can be used as an additional experimental model, assuring a high- throughput of experiments using genetic manipulation owing to shorter period of growing.ObjectiveTo examine mechanical properties and tissue structure of tendon in immature Iberian ribbed newt following complete transection.MethodsDigital flexor tendon of the middle finger of the left hindlimb in 4- and 6-month-old Iberian ribbed newt (4mo and 6mo, respectively) was transected. Regenerated tendon was mechanically tested at 6 and 12 weeks postoperatively. Collagen fiber structure was also observed using two-photon microscopy.ResultsIn both 4mo and 6mo newts, regenerated tendon at 6 weeks exhibited significantly lower tensile strength than corresponding normal tendons and had unorganized collagen structure. At 12 weeks, Regenerated tendon in both groups had the strength comparable to normal controls. Additionally, the collagen structure seemed more organized compared to that at 6 weeks and comparable to controls. These phenomena were essentially similar to those in adult newts.Conclusion4mo and 6mo newts can also be used as experimental models of adult tendon regeneration research.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"9592989251324288"},"PeriodicalIF":1.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-scale topology optimisation design and mechanical property analysis of porous interbody fusion cage.","authors":"Huaiyue Zhang, Rongchang Fu, Xu Zhu","doi":"10.1177/09592989241303291","DOIUrl":"10.1177/09592989241303291","url":null,"abstract":"<p><p>BackgroundTitanium (Ti) and polyether ether ketone (PEEK) interbody fusion cages cause postoperative stress shielding problems. The porous cage design is one of the solutions advanced to mitigate this problem.ObjectiveExploring the mitigation of stress shielding with a porous interbody fusion cage after surgery for idiopathic scoliosis.MethodsThe porous interbody fusion cage was constructed based on the multiscale topology optimisation method, and the postoperative lumbar spine models implanted with it. The porous Ti and PEEK fusion cages were evaluated under physiological conditions to investigate their mechanical properties.ResultsThe volume of the porous fusion cage was reduced by 52.57%, and the stress was increased by 242.76% and 252.46% compared with the Ti and PEEK fusion cage; the modulus of elasticity of the porous fusion cage was reduced by 76.85%, and the strain was increased by 131.40%∼686.51% compared with the Ti cage; the porous fusion cage increased L3 cortical bone stress by 13.36% and 13.52% and cancellous bone by 82.93% and 76.72%, respectively, compared with the original interbody fusion cages.ConclusionThe porous interbody fusion cage has a much more lightweight design which facilitates growth of bone tissue. However, a frame structure should be constructed to minimize issues with stress peaks and localised stress concentrations. It also has a significantly lower stiffness which helps alleviate vertebral stress shielding, further fostering bone growth. The porous fusion cage thus meets the clinical requirements for better fusion outcomes.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"110-123"},"PeriodicalIF":1.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polydopamine-functionalized nanohydroxyapatite coated exosomes with enhanced cytocompatibility and osteogenesis for bone regeneration.","authors":"Mingli Xiang, Gengchao Zhang, Yulin Liu, Chengcheng Liao, Linlin Xiao, Meiling Xiang, Xiaoyan Guan, Jianguo Liu","doi":"10.1177/09592989241301662","DOIUrl":"10.1177/09592989241301662","url":null,"abstract":"<p><p>BackgroundMesenchymal stem cells-derived exosomes, crucial in regenerative medicine, have been explored for their potential for the functional modification of bone scaffolds.ObjectiveTo design a functionally modified biomimetic nanohydroxyapatite using exosomes and explore its effects on bone regeneration.MethodsA biomimetic nanohydroxyapatite (named as tHA) was fabricated as previous methods using a polydopamine (pDA) structure as a template, and exosomes (Exo) derived from periodontal ligament stem cells (PDLSCs) were used to functionally modify the tHA scaffold material through pDA. The effects of functional composite scaffold (tHA-Exo) on cells proliferation and osteogenic differentiation were investigated. Furthermore, their effect on bone regeneration was also evaluated in vivo.ResultsExosomes can be loaded onto the tHA via pDA and the tHA-Exo releases exosomes in a sustained and stable manner. tHA-Exo showed improved cytocompatibility compared to controls. Additionally, tHA-Exo significantly enhanced the proliferation and osteogenic differentiation of PDLSCs. More importantly, animal experiments have shown that tHA-Exo could dramatically promote bone regeneration.ConclusionThe tHA nanoparticles, functionally modified by the PDLSCs-Exo through pDA, significantly promoted bone regeneration by improving its cytocompatibility and osteogenic potential, which could serve as a promising material for promoting bone regeneration.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"98-109"},"PeriodicalIF":1.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel vessel enhancement method based on Hessian matrix eigenvalues using multilayer perceptron.","authors":"Xiaoyu Guo, Jiajun Hu, Tong Lu, Guoyin Li, Ruoxiu Xiao","doi":"10.1177/09592989241296431","DOIUrl":"10.1177/09592989241296431","url":null,"abstract":"<p><strong>Background: </strong>Vessel segmentation is a critical aspect of medical image processing, often involving vessel enhancement as a preprocessing step. Existing vessel enhancement methods based on eigenvalues of Hessian matrix face challenges such as inconsistent parameter settings and suboptimal enhancement effects across different datasets.</p><p><strong>Objective: </strong>This paper aims to introduce a novel vessel enhancement algorithm that overcomes the limitations of traditional methods by leveraging a multilayer perceptron to fit a vessel enhancement filter function using eigenvalues of Hessian matrix. The primary goal is to simplify parameter tuning while enhancing the effectiveness and generalizability of vessel enhancement.</p><p><strong>Methods: </strong>The proposed algorithm utilizes eigenvalues of Hessian matrix as input for training the multilayer perceptron-based vessel enhancement filter function. The diameter of the largest blood vessel in the dataset is the only parameter to be set.</p><p><strong>Results: </strong>Experiments were conducted on public datasets such as DRIVE, STARE, and IRCAD. Additionally, optimal parameter acquisition methods for traditional Frangi and Jerman filters are introduced and quantitatively compared with the novel approach. Performance metrics such as AUROC, AUPRC, and DSC show that the proposed algorithm outperforms traditional filters in enhancing vessel features.</p><p><strong>Conclusion: </strong>The findings of this study highlight the superiority of the proposed vessel enhancement algorithm in comparison to traditional methods. By simplifying parameter settings, improving enhancement effects, and showcasing superior performance metrics, the algorithm offers a promising solution for enhancing vessel parts in medical image analysis applications.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"83-97"},"PeriodicalIF":1.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of silk short fiber biomimetic materials on the recovery of sports function in patients with meniscal injury during sports.","authors":"Yong Wang, Dongsong Bin","doi":"10.1177/09592989241296427","DOIUrl":"10.1177/09592989241296427","url":null,"abstract":"<p><p>BackgroundIn sports, especially high-intensity and high-risk activities, the meniscus is easily damaged. For patients with meniscus injuries, it is necessary to repair or replace the patient's meniscus. However, as age increases, the human meniscus tissue gradually forms and cannot be repaired through its own meniscus. Therefore, it is necessary to maintain the patient's movement function through meniscus support materials.ObjectiveTraditional meniscus support materials have poor mechanical properties and poor biocompatibility. In response to this issue, this study designed a meniscus scaffold made of silk short fibers, silk fibroin, and wool protein.MethodsThrough electrospinning and freeze-drying techniques, the material was processed to obtain a silk short fiber meniscus with a biomimetic structure.ResultsThrough experiments, the surface morphology, hydrophobicity, porosity, secondary structure, thermal stability, water absorption swelling, and MP of MCS made of SSF biomimetic materials were characterized.ConclusionThe experimental results show that the manufactured silk short fiber meniscus has good compressive performance, thermal stability, and water absorption and swelling properties, and it also exhibits good biocompatibility.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"69-82"},"PeriodicalIF":1.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}