Bio-medical materials and engineering最新文献_第3页

Effect of fat thickness on subcutaneous temperature field under monopolar radiofrequency. 单极射频下脂肪厚度对皮下温度场的影响。

IF 1.3 4区医学

Bio-medical materials and engineering Pub Date : 2026-03-01 Epub Date: 2026-01-19 DOI: 10.1177/09592989251407753

Ping Ye, Jiawen Zong, Qinghua Kang, Chunping Liu, Baosan Han

{"title":"Effect of fat thickness on subcutaneous temperature field under monopolar radiofrequency.","authors":"Ping Ye, Jiawen Zong, Qinghua Kang, Chunping Liu, Baosan Han","doi":"10.1177/09592989251407753","DOIUrl":"10.1177/09592989251407753","url":null,"abstract":"BackgroundMonopolar Radiofrequency uses high-frequency waves to generate heat for skin tightening and tissue repair. However, individual fat layer thickness variation causes uneven radiofrequency (RF) penetration and heat thresholds, compromising personalized results.ObjectiveThe purpose of this study is to analyze the temperature distribution of tissues with different fat thickness after radio frequency treatment and the experimental temperature distribution and tissue changes of pork tissues in vitro by finite element analysis and in vitro experiment verification, so as to achieve appropriate energy parameters for different individuals.MethodsA two-dimensional bio-thermal model including epidermis, dermis and subcutaneous tissue was developed in COMSOL Multiphysics 6.2. Four fat thicknesses (2, 4, 6, and 8 mm) were simulated to assess their impact on dermal temperature distribution during 6.78 MHz, 120 W radiofrequency exposure. The electromagnetic-thermal coupling effects were validated through in vitro experiments.ResultsExperimental results validate the simulations, demonstrating consistent thermal trends across fat thicknesses (2-8 mm). Post-treatment intratissue temperatures reached 69 °C (2 mm), 60 °C (4 mm), 55 °C (6 mm), and 45 °C (8 mm), all within epidermal safety limits.ConclusionThe results show that the energy parameters need to be adjusted according to the thickness of adipose tissue during radiofrequency therapy, and higher energy or longer treatment time may be needed for the treatment site with thicker adipose tissue to achieve the expected effect.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"82-92"},"PeriodicalIF":1.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146003096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in polymerization stress of self-adhesive resin composites during water storage. 自粘树脂复合材料储水过程中聚合应力的变化。

IF 1.3 4区医学

Bio-medical materials and engineering Pub Date : 2026-03-01 Epub Date: 2025-10-17 DOI: 10.1177/09592989251382362

Kazuyoshi Okawa, Daichi Aizawa, Hiro Matsumoto, Masao Hanabusa, Takatsugu Yamamoto

{"title":"Changes in polymerization stress of self-adhesive resin composites during water storage.","authors":"Kazuyoshi Okawa, Daichi Aizawa, Hiro Matsumoto, Masao Hanabusa, Takatsugu Yamamoto","doi":"10.1177/09592989251382362","DOIUrl":"10.1177/09592989251382362","url":null,"abstract":"BackgroundResin composite restorations exhibit dimensional changes in the oral environment due to polymerization reaction and/or water sorption, which generates stresses in the surrounding tooth structures. The state of stress may differ between self-adhesive resin composites (SARCs) and conventional resin composites because only SARCs contain hydrophilic monomers.ObjectiveThe objective of this study was to evaluate the influence of water sorption on polymerization stresses of SARCs.MethodsCracks were introduced near a cylindrical hole in a glass disk, and their lengths were measured. The hole was filled with the composites. The crack lengths were repeatedly measured during 1-week water storage 37°C. Stresses at the composite-glass interface were calculated from the crack lengths and fracture toughness of the glass. Flexural moduli, water sorption and solubility of composites were also measured.ResultsPolymerization stresses of SARCs were equivalent to or less than that of a conventional composite generating relatively little stress. Significant reduction of stress occurred between 1-h and 1-day water storage in all composites. This reduction tended to be more noticeable with a larger decrease in modulus and/or larger water sorption.ConclusionsQuicker and/or larger stress reduction were considered to be beneficial for the longevity of SARC restorations.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"65-73"},"PeriodicalIF":1.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145312447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Near-infrared spectroscopy and machine-learning evaluation of PVA/β-TCP biomaterial composites. PVA/β-TCP生物材料复合材料的近红外光谱和机器学习评价。

IF 1.3 4区医学

Bio-medical materials and engineering Pub Date : 2026-03-01 Epub Date: 2026-02-02 DOI: 10.1177/09592989251414479

Yuta Otsuka, Tomohiro Masuzaki, Hayato Takase, Toru Naito

{"title":"Near-infrared spectroscopy and machine-learning evaluation of PVA/β-TCP biomaterial composites.","authors":"Yuta Otsuka, Tomohiro Masuzaki, Hayato Takase, Toru Naito","doi":"10.1177/09592989251414479","DOIUrl":"10.1177/09592989251414479","url":null,"abstract":"A composite of polyvinyl alcohol (PVA) and beta-tricalcium phosphate (β-TCP) was synthesized as a biomaterial filament for 3D printers and its analytical and chemical evaluation was performed. PVA powder and β-TCP were mixed in the range of 0-20 wt% and hot-melt extruded at 200 °C using a single-screw extruder. Comprehensive material characterization of the synthesized filament was performed by powder X-ray diffraction (XRD), near-infrared spectroscopy (NIR), and scanning electron microscopy (SEM). XRD analysis confirmed that the amorphous nature of PVA and the crystalline nature of β-TCP coexisted and the physical mixture state was well maintained. In near-infrared spectroscopy, concentration-dependent spectral changes were observed by normalization, and principal component analysis showed that the first principal component explained 85.6% of the variance. In machine learning regression analysis, partial least squares regression (PLS), random forest (RF), and support vector machine (SVM) were compared, and SVM achieved the best prediction accuracy (R2 = 0.910). SEM observations confirmed streaky structures along the extrusion direction and uniform dispersion of β-TCP particles. This study demonstrated that a combined NIR spectroscopy and machine learning approach is effective as a non-destructive quality evaluation technique for composite filaments for 3D printing. This technique enables real-time composition monitoring and quality control of biomaterial filaments, and is expected to be applied to the manufacturing of patient-specific biomedical devices.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"117-128"},"PeriodicalIF":1.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146104069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced decellularization efficiency in combination of low-concentration chemical and physical methods on the human umbilical arteries. 结合低浓度化学和物理方法在人脐动脉上增强脱细胞效率。

IF 1.3 4区医学

Bio-medical materials and engineering Pub Date : 2026-03-01 Epub Date: 2026-01-19 DOI: 10.1177/09592989251397800

Nho Thuan Nguyen, Tung Ngoc Hoang Pham, Hoang Minh Lam, Tuyet Thi Diem Hoang, Thang Quoc Bui, Ha Le Bao Tran

{"title":"Enhanced decellularization efficiency in combination of low-concentration chemical and physical methods on the human umbilical arteries.","authors":"Nho Thuan Nguyen, Tung Ngoc Hoang Pham, Hoang Minh Lam, Tuyet Thi Diem Hoang, Thang Quoc Bui, Ha Le Bao Tran","doi":"10.1177/09592989251397800","DOIUrl":"10.1177/09592989251397800","url":null,"abstract":"BackgroundCardiovascular diseases are the leading cause of mortality worldwide, with coronary artery bypass grafting being the most effective treatment for severe cases. While autografts are preferred, donor veins are often limited. Human umbilical arteries (hUAs) show promise as an alternative. However, to make vascular graft by decellularization, a traditional chemical method can damage tissue structure and function.ObjectiveThis study aims to evaluate the shortening of treatment time and the hUA decellularization efficiency of the perfusion bioreactor systems.MethodshUAs were perfused with 1% Triton X-100 for 6 h, followed by two different concentrations of (0.5% and 1%) SDS for 18 h, and subsequently subjected to a washing procedure. The decellularization process was evaluated using histological staining and DNA quantification, along with tests for cytotoxicity, cell adhesion and proliferation.ResultsThe 0.5% SDS protocol proved most effective, reducing residual DNA to below 50 ng/mg of dry weight while preserving collagen structure. It showed no cytotoxicity to L929 cells, SEM analysis confirmed human umbilical vein endothelial cell (HUVEC) attachment and CCK-8 testing showed promoted HUVECs proliferation.ConclusionThe decellularization protocol of perfusing through 1% TX for 6 h and 0.5% SDS for 18 h through the perfusion bioreactor system is efficient in the intact hUAs tissue. This sets the stage for future in vivo studies and potential clinical applications.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"74-81"},"PeriodicalIF":1.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145997209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lumbar stress distribution in sitting posture with considering spinal alignment: A finite element analysis. 腰椎应力分布在坐姿与考虑脊柱对齐：一个有限元分析。

IF 1.3 4区医学

Bio-medical materials and engineering Pub Date : 2026-01-01 Epub Date: 2025-07-13 DOI: 10.1177/09592989251358151

Ryota Toyohara, Takahiro Hiramukai, Toshiro Ohashi

{"title":"Lumbar stress distribution in sitting posture with considering spinal alignment: A finite element analysis.","authors":"Ryota Toyohara, Takahiro Hiramukai, Toshiro Ohashi","doi":"10.1177/09592989251358151","DOIUrl":"10.1177/09592989251358151","url":null,"abstract":"BackgroundSitting on a chair is an essential part of daily life, however the posture is a heavy burden on the lower back, leading to lower back pain.ObjectiveIn this study, lumbar stress distribution was analyzed when sitting on a chair with different backrest inclination, sitting distance and seat pan inclination in order to visualize the stress on intervertebral discs and sacroiliac joints (SIJs).MethodsBody pressure distribution was measured on backrests and seat pans of six subjects. Each sitting posture on a chair was reproduced using a finite element model of a spine and pelvis, and the stress distribution in the lower back was analyzed based on the results of the body pressure distribution measurements.ResultsWhen the seat pan was tilted forward, the pressure on the backrest decreased, whereas the pressure increased with large sitting distance. Finite element analyses showed that equivalent stresses on the SIJs and intervertebral discs was decreased when sitting on a chair shallowly and with the seat pan tilted forward.ConclusionBoth sitting shallowly and with a forward-tilted seat are postures that increase the trunk-thigh angle, and the load on the lower back was reduced by reducing the moment applied to the sacrum.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"42-49"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144625371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental and numerical failure analysis of Thompson hip prosthesis. Thompson髋关节假体的实验与数值失效分析。

IF 1.3 4区医学

Bio-medical materials and engineering Pub Date : 2026-01-01 Epub Date: 2025-06-30 DOI: 10.1177/09592989251353462

Ali Motameni, Ömer Can Farukoğlu, Rıza Gürbüz, İhsan Korkut

{"title":"Experimental and numerical failure analysis of Thompson hip prosthesis.","authors":"Ali Motameni, Ömer Can Farukoğlu, Rıza Gürbüz, İhsan Korkut","doi":"10.1177/09592989251353462","DOIUrl":"10.1177/09592989251353462","url":null,"abstract":"BackgroundThere are different surgical procedures to fix femoral head-neck fractures, such as total hip arthroplasty and hemiarthroplasty. Under optimal conditions, the inserted prosthesis should last a long time, possibly a lifetime, with only trace amounts of wear each year due to the friction between the femur bone and the prosthesis stem. However, in some cases, failure due to excessive wear, creep, fatigue, corrosion, etc., can occur.ObjectiveThis study investigates the failure causes of a Thompson hip prosthesis both experimentally and numerically.MethodsThe stem of the prosthesis, which is inserted into the femur, broke inside a patient's body over time. Following the removal surgery, the severely damaged and fractured stem was examined to determine the root causes of the failure. For this purpose, fractographic examination of the fractured surfaces was conducted with scanning electron microscope (SEM). Microstructural analyses were performed using optical microscope, and the chemical composition of the prosthesis was analyzed with energy dispersive spectroscopy (EDS). Vickers hardness (HV30) test was conducted on the femoral stem. Additionally, finite element method (FEM) was applied to estimate the forces acting on the prosthesis.ResultsIt was observed that fatigue cracks initiated and propagated from the outer surface of the femoral stem at locations very close to the maximum von Mises stresses observed in the FEM analysis. However, the load magnitudes in the FEM analysis were not high enough to initiate any cracks.ConclusionIt is considered that crack initiation occurred due to material defects from the prosthesis manufacturing process, and the cyclic loading during body motion propagated these cracks. Ultimately, the fracture of the Thompson hip prosthesis occurred.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"33-41"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mechanical properties of bone filling materials affect the therapeutic effect of vertebroplasty: Biomechanical finite element analysis. 骨填充材料的力学性能影响椎体成形术的治疗效果：生物力学有限元分析。

IF 1.3 4区医学

Bio-medical materials and engineering Pub Date : 2026-01-01 Epub Date: 2025-07-28 DOI: 10.1177/09592989251353447

Xiangjing Zeng, Cheng Long, Yuting Yi, Shaoru Lin

{"title":"The mechanical properties of bone filling materials affect the therapeutic effect of vertebroplasty: Biomechanical finite element analysis.","authors":"Xiangjing Zeng, Cheng Long, Yuting Yi, Shaoru Lin","doi":"10.1177/09592989251353447","DOIUrl":"10.1177/09592989251353447","url":null,"abstract":"BackgroundBone filling materials that match the mechanical properties of normal cancellous bone may be more suitable for vertebroplasty to improve the complications caused by osteoporosis.ObjectiveTo prepare and evaluate a new bone filling material (NBFM) that matches the mechanical properties of normal cancellous bone for vertebroplasty.MethodsA new bone filling material (NBFM) was prepared and its biomechanical properties were compared with those of polymethyl methacrylate (PMMA) bone filling material commonly used in clinical vertebroplasty. Finite element analysis was conducted to compare the biomechanical differences between NBFM and PMMA. The lumbar spine model's biomechanical differences were assessed under four different loading conditions: flexion, extension, left flexion, and right flexion.ResultsThe NBFM demonstrated biomechanical properties more closely matching normal cancellous bone compared to PMMA. The finite element analysis revealed that the lumbar spine model with NBFM exhibited improved biomechanical behavior under the specified loading conditions.Conclusion:Bone filling materials that match the mechanical properties of normal cancellous bone, such as the newly developed NBFM, are more suitable for vertebroplasty and may help reduce complications associated with osteoporosis.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"13-23"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144727940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of coamorphization on meloxicam/saccharine: Intermolecular interaction investigations using chemometrics. 共定型对美洛昔康/糖精的影响：利用化学计量学研究分子间相互作用。

IF 1.3 4区医学

Bio-medical materials and engineering Pub Date : 2026-01-01 Epub Date: 2025-07-21 DOI: 10.1177/09592989251341175

Yuta Otsuka

{"title":"Effect of coamorphization on meloxicam/saccharine: Intermolecular interaction investigations using chemometrics.","authors":"Yuta Otsuka","doi":"10.1177/09592989251341175","DOIUrl":"10.1177/09592989251341175","url":null,"abstract":"Background: Aqueous solubility of pharmaceuticals is a factor as it is directly associated with bioavailability; accordingly, strategies to enhance solubility have been well investigated. Objectives: The purpose of this study was to determine the effects of coamorphization on meloxicam (MX) and saccharine (SA) mixtures. Methods: An equimolar mixture of MX and SA was ground for 4 h at 300 rpm. The obtained samples were evaluated using Fourier-transform mid-infrared spectroscopy, Fourier transform near-infrared spectroscopy, powder X-ray diffraction (PXRD), and thermal analysis. No molecular interactions were observed in the physical mixture sample. The ground samples showed broad peaks in the PXRD patterns and an exothermic peak at an early temperature. Results: The results suggested that the grinding process transformed MX and SA into a coamorphous phase. The attenuated total reflection - IR spectra exhibited new peaks at 1719 cm-1 and 1398 cm-1, and the NH peak disappeared with grinding time. Measurement data of MX and SA ground sample suggested they constructed coamorphous phase. Conclusion: It was indicated by multivariate analysis that the formation of the MX/SA coamorphous system occurred in a two-step process.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"3-12"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lumefantrine co-amorphous systems using deoxycholic acid as a co-former: NIR at-line process monitoring by machine learning. 以脱氧胆酸为共成体的苯丙啶共非晶态体系：通过机器学习的近红外在线过程监测。

IF 1.3 4区医学

Bio-medical materials and engineering Pub Date : 2026-01-01 Epub Date: 2025-08-04 DOI: 10.1177/09592989251353457

Yuta Otsuka, Kiyotada Naitou, Takeshi Miyata

{"title":"Lumefantrine co-amorphous systems using deoxycholic acid as a co-former: NIR at-line process monitoring by machine learning.","authors":"Yuta Otsuka, Kiyotada Naitou, Takeshi Miyata","doi":"10.1177/09592989251353457","DOIUrl":"10.1177/09592989251353457","url":null,"abstract":"BackgroundVarious methods have been reported for improving the water-insoluble drugs in oral administration formulations. Among them, amorphization has been attracting attention and developed as a method for solubilizing API (active pharmaceutical ingredient)s by changing their physicochemical properties. Molecular complexation is also known as a method for solubilizing APIs by synthesizing cocrystals, etc. Co-amorphization, which achieves both molecular complexation and amorphization, is effective and has attracted attention. Thus, co-amorphization has been proven to be an effective approach to solubilization.ObjectiveThis study aims to improve the solubility of lumefantrine, used here as a model compound, through co-amorphization with deoxycholic acid.SignificanceThe physicochemical properties are an important factor in developing pharmaceutical ingredients. Hydrogen-bonded co-amorphization has gained attention as a method to enhance the physicochemical properties of hydrophobic drugs.MethodsThe co-amorphous Lumefantrine-deoxycholic acid system was prepared using a mechanochemical synthesis method based on ball milling. The synthesis process was monitored by powder X-ray diffraction and near-infrared spectroscopy. The products and materials were analyzed by thermal analysis.ResultsSpectroscopic analysis revealed that the two molecules were complexed through intermolecular hydrogen bonding interactions. The produced co-amorphous has no melting point was found by thermal analysis.ConclusionsProcess monitoring also indicated the presence of a metastable crystalline Lumefantrine (LMF) intermediate.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"24-32"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144783494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeted nanocarriers effectively remove cholesterol from macrophages. 靶向纳米载体可有效去除巨噬细胞中的胆固醇。

IF 1.3 4区医学

Bio-medical materials and engineering Pub Date : 2026-01-01 Epub Date: 2025-09-12 DOI: 10.1177/09592989251375811

Jin Chen, Qing Miao, Xinrong Xiao

{"title":"Targeted nanocarriers effectively remove cholesterol from macrophages.","authors":"Jin Chen, Qing Miao, Xinrong Xiao","doi":"10.1177/09592989251375811","DOIUrl":"10.1177/09592989251375811","url":null,"abstract":"BackgroundMacrophages phagocytose large amounts of cholesterol to form foam cells that can aggravate inflammation and further promote the development of atherosclerotic plaque.ObjectiveTo develop novel nanocarriers targeting atherosclerosis-associated macrophages.MethodsCD-G5 was obtained by modifying β-CD onto PAMAM G5.0, and subsequently PEG2000 was used as a linker arm to modify mannose onto PAMAM G5.0 of CD-G5 to obtain CD-G5-PEG-Man. CD-G5-PEG-Man was structurally characterized and evaluated in vitro for its cell biological functions.ResultsCD-G5-PEG-Man had an average particle size of 110 nm and a regular spherical morphology. CD-G5-PEG-Man showed no significant toxicity to macrophages at all the experimental concentration gradients. Macrophages showed stronger uptake of the fluorescently labelled nanoparticle CD-G5-PEG-Man-FITC than CD-G5-FITC, and the fluorescence weakened with increasing free mannose. Intracellular BODIY-cholesterol fluorescence intensity was weaker in the 200 nM CD-G5-PEG-Man treatment group than in the 100 µM HP-β-CD, 100 nM CD-G5-PEG-Man, and DMSO treatment groups. The higher the amount of β-CD on the CD-G5-PEG-Man, the lower the fluorescence intensity of intracellular BODIY-cholesterol.ConclusionA biosafety nanocarrier, CD-G5-PEG-Man, was successfully developed, in which mannose specifically targets macrophages via mannose receptors on macrophages, and β-CD synergistically promotes cholesterol efflux from macrophages.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"50-61"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145051848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0