Bio-medical materials and engineering最新文献

{"title":"Hepatitis nursing robot based on RP-lidarA1 sensor.","authors":"Yunxia Dai, Bei Xue, Qian Zhang, Juxiang Shao","doi":"10.1177/09592989241313110","DOIUrl":"https://doi.org/10.1177/09592989241313110","url":null,"abstract":"<p><strong>Background: </strong>To alleviate the workload of medical staff and provide personalized care for hepatitis patients, this study focuses on developing a hepatitis care robot.</p><p><strong>Objective: </strong>The objective of this study is to integrate the RP-lidarA1 sensor into a hepatitis care robot to achieve high-precision environmental perception, mapping, and navigation, thereby improving healthcare services.</p><p><strong>Methods: </strong>The RP-lidarA1 sensor was utilized for environmental scanning, and the MPU6050 chip was used to collect attitude data. An improved RBPF-SLAM algorithm was employed for high-precision map construction. For positioning and navigation, a combination of the A* algorithm and Dynamic Window Approach (DWA) algorithm was used to optimize path planning and obstacle avoidance.</p><p><strong>Results: </strong>Simulation experiments demonstrated that the improved algorithm reduced the number of particles to 50 in a 140 m² area and shortened the map construction time to 1200 s. The A* algorithm effectively planned optimal paths, while the DWA algorithm improved navigation efficiency. Satisfaction surveys indicated that 92.4% of hepatitis patients and 81.8% of nurses were highly satisfied with the robot's performance.</p><p><strong>Conclusions: </strong>The hepatitis care robot integrating the RP-lidarA1 sensor showed excellent performance in autonomous navigation, map construction, and obstacle avoidance, significantly enhancing the quality and efficiency of medical services.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"9592989241313110"},"PeriodicalIF":1.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced detection of Mycobacterium Tuberculosis using nanogold-based silver staining enhancement.","authors":"Lili Quan, Qianrong Yang, Zhu Jin, Rong Li, Han Xiao, Lu Wang","doi":"10.1177/09592989241308792","DOIUrl":"https://doi.org/10.1177/09592989241308792","url":null,"abstract":"<p><strong>Background: </strong>Tuberculosis (TB) is a global health challenge from a single infectious agent, Mycobacterium tuberculosis (MTB), and it demands improved diagnostics and therapies.</p><p><strong>Objective: </strong>This work explored a novel method for detecting MTB by combining nanogold labeling (NGL) technology with silver staining to enhance sensitivity and specificity.</p><p><strong>Methods: </strong>Nanogold particles (NGPs) were characterized using ultraviolet absorption spectroscopy (UVAS), and their morphology was observed via transmission electron microscopy (TEM). The silver staining enhancement (SSE) system was optimized for a reaction time of 11 min. Fifty drug-resistant tuberculosis (DRT) patients were randomly assigned to a control (Ctrl) group receiving conventional nursing and an experimental (Exp) group treated with continuous nursing intervention (CNI). Quality of Life Instrument for Tuberculosis Patients (QLI-TB) scores were compared over 6 months.</p><p><strong>Results: </strong>Unmarked NGPs were evenly distributed, while labeled NGPs maintained complete morphology with a gray halo. The detection limit was established at 0.582, reaching as low as 1 pmol/L. For sputum specimens, detection rates were 38.7% for culture, 41.94% for PCR, and 43.54% for nanogold SSE, with no significant differences (<i>P </i>> 0.05). However, patients in the Exp group exhibited significant improvements in physical, psychological, and social functions, as well as the tuberculosis-specific module (TSM) compared to the Ctrl group (<i>P </i>< 0.05).</p><p><strong>Conclusions: </strong>We demonstrated an innovative method for detecting MTB, demonstrating promising results through method optimization and analysis.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"9592989241308792"},"PeriodicalIF":1.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre-alignment of fibroblasts induced by micropatterning and cyclic stretching techniques accelerates wound healing.","authors":"Ryota Toyohara, Yanan Lan, Yu Chen, Takaya Yoriki, Toshiro Ohashi","doi":"10.1177/09592989241305522","DOIUrl":"https://doi.org/10.1177/09592989241305522","url":null,"abstract":"<p><strong>Background: </strong>Wound healing proceeds through a complex collaborative process. It has been shown that during the intermediate phase of the wound healing process, fibroblasts migrate into wound area and contract to contribute to the closure of the wound area. Moreover, previous studies have shown that fibroblast alignment was observed on the mature stage of wound scar. These studies clearly indicate that fibroblasts play a critical role in wound healing process, however, the whole mechanism of wound healing remains still unclear.</p><p><strong>Objective: </strong>Fibroblasts are pre-aligned to evaluate the effect of cell alignment on cell migration rate.</p><p><strong>Methods: </strong>The cell alignment was accomplished by PDMS microstamping with fibronectin and application of cyclic stretching. Wound was created by physical scratching and then the wound closure rate was measured.</p><p><strong>Results: </strong>The pre-aligned cells perpendicular to the direction of scratched wound exhibited significantly higher migration rate, compared to non-aligned control cells. Moreover, pre-aligned cells with thick actin filaments by cyclic stretching migrated faster than those with less development of actin filament structures by microstamping.</p><p><strong>Conclusion: </strong>The wound closure can be accelerated by the adequate alignment of fibroblasts as well as the development of actin filament structures.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"9592989241305522"},"PeriodicalIF":1.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental study of extracellular vesicle-small intestine submucosa novel biological composite for urethral stricture repair and reconstruction.","authors":"Dan Wang, Xiaojun Zhu, Buhe Siqin, Chao Ren, Ming Chang, Ligang Bai","doi":"10.1177/09592989241308802","DOIUrl":"https://doi.org/10.1177/09592989241308802","url":null,"abstract":"<p><strong>Background: </strong>Urethral stricture (US) is a common condition that considerably affects patients' quality of life.</p><p><strong>Objectives: </strong>This study aimed to explore the adoption value of the extracellular vesicle (EV)- small intestinal submucosa (SIS) complex in the repair of USs.</p><p><strong>Methods: </strong>EVs were extracted from healthy male New Zealand white rabbits, and SIS was prepared using peracetic acid (PAA) oxidation and decellularization. The morphology and particle size of the prepared EV-SIS complex were evaluated using electron microscopy and qNano nanoparticle analyzer, and the labeled proteins of EVs were detected using Western blot method. EV-SIS the complex was implanted in a rabbit model of US, and urodynamic parameters were assessed.</p><p><strong>Results: </strong>The EV-SIS complex displayed a full morphology, intact membrane structure, and uniform particle size. The protein concentration of EVs in the complex was approximately 0.351 µg/µL, with a yield of approximately 1.86 µg/10⁶ cells. The complex exhibited remarkable repair effects in the rabbit model of US, with bladder capacity, maximal urethral pressure, and minimal urethral pressure all markedly superior to those in the US group (<i>P </i>< 0.05).</p><p><strong>Conclusion: </strong>The EV-SIS complex demonstrates potential clinical value in the repair of USs, improving urodynamic parameters, and offering a promising therapeutic option for patients with US.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"9592989241308802"},"PeriodicalIF":1.0,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomechanical and clinical assessment of dissociation in bipolar hip hemiarthroplasty.","authors":"Fırat Ozan, Kürşat Tuğrul Okur, Fırat Mavi, Mahmut Pekedis","doi":"10.1177/09592989241306688","DOIUrl":"https://doi.org/10.1177/09592989241306688","url":null,"abstract":"<p><strong>Background: </strong>Dissociation is a special type of dislocation that is rarely seen in bipolar hemiarthroplasty.</p><p><strong>Objective: </strong>To investigate the clinical and biomechanical causes of dissociative dislocation of acetabular cup components in the hips of patients after bipolar hemiarthroplasty (BHA).</p><p><strong>Methods: </strong>BHA heads were divided into three groups according to their design. Cam-out and pull-out biomechanical tests were conducted to investigate the separation strengths of the BHA heads.</p><p><strong>Results: </strong>Among the 1684 BHA surgeries performed, the revision surgery rate was 4% (68 hips) and dissociation rate was 0.8% (15 hips). According to the cam-out test result, the highest values in the parameters ultimate force (F_max) ultimate torque (T), vertical displacement at maximum force (δ_m), rotation at maximum force (ϕ_m), maximum principal strain (Ɛ_max), minimum principal strain (Ɛ_min), average normal strain (Ɛ_av), maximum shear strain (γ_max), uniaxial normal strain (Ɛ_un) were detected in Type 2 BHA head. The pull-out values of the implants were sufficient to prevent the distraction force that may occur on the implant due to manual traction applied to the hip dislocations. However, in closed reduction maneuvers, cam-out-shaped deformation forces may cause dissociation in Types 1 and 3, but not in Type 2 BHA heads. According to the pull-out test results, while high values were detected in the parameters Fmax and stiffness (<i>k</i>) in the Type 2 BHA head, δm and maximum force (E) parameters were found to be high in the Type 1 BHA head. In the cam-out test, a strong positive relationship was found between the thickness and width of the polyethylene locking ring and F_max, T, δ_m, ϕ_m, Ɛ_max, γ_max, Ɛ_un.</p><p><strong>Conclusion: </strong>Better BHA head designs and polyethylene designs may help resolve the rare problem of dissociation, which almost always leads to resurgical procedures.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"9592989241306688"},"PeriodicalIF":1.0,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing biomaterial application strategies for neurorestoration after ischemic stroke injury: An emerging treatment option.","authors":"Asmaa' Mohd Satar, Farah Amna Othman, Suat Cheng Tan","doi":"10.1177/09592989241306679","DOIUrl":"https://doi.org/10.1177/09592989241306679","url":null,"abstract":"<p><strong>Background: </strong>Ischemic stroke is a medical condition caused by occlusion of blood vessels in brain, resulting in disruption of blood flow to the brain and triggering irreversible damage to the neuronal cells. While stem cells transplantation has been proposed as a potential alternative therapym for ischemic stroke, its effectiveness is limited due to low cell survival rate and potential side effects following transplantation. To overcome these challenges and enhance therapeutics efficacy, researchers have focused on developing various biomaterials to create a sustainable cellular microenvironment or to modify the properties of donor stem cell which could optimize their reparative functions in injured brain tissues.</p><p><strong>Objective: </strong>This review aims to explore and discuss the different types of biomaterials that have been applied in the treatment of ischemic stroke, shedding light on their potentials as promising therapeutics options for this debilitating condition.</p><p><strong>Methods: </strong>Literature search was performed to identify publications studying the potential of three biomaterials namely: nanobioparticles, hydrogels and extracellular vesicles for ischemic stroke therapy in vitro, in vivo or in clinical using four databases, namely: PubMed, ScienceDirect, Web of Science and Scopus.</p><p><strong>Results and discussion: </strong>The major benefits obtained from the application of nanobioparticles for ischemic stroke therapy included as the nanocarrier for drug/cell delivery, cell tracking, real time imaging, promote cell proliferation, while hydrogels provided scaffold support and conferred neuroprotection to stem cells, as well as provided neurotropic effects and controlled drug release for localized treatment. Lastly the extracellular vesicles were identified as a cell-free treatment strategy in promoting angiogenesis, neuronal differentiation and neurogenesis for ischemic stroke treatment.</p><p><strong>Conclusion: </strong>Biomaterial-based therapies have their own potentials and further clinical investigations are strongly recommended to translate the therapies into more conscientious evidence-based therapy for clinical application.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"9592989241306679"},"PeriodicalIF":1.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promotion of maturation in CDM3-induced embryonic stem cell-derived cardiomyocytes by palmitic acid.","authors":"Junsheng Mu, Zhen Gao, Ping Bo, Bin You","doi":"10.3233/BME-240101","DOIUrl":"10.3233/BME-240101","url":null,"abstract":"<p><strong>Background: </strong>Myocardial infarction leads to myocardial necrosis, and cardiomyocytes are non-renewable. Fatty acid-containing cardiomyocyte maturation medium promotes maturation of stem cell-derived cardiomyocytes.</p><p><strong>Objective: </strong>To study the effect palmitic acid on maturation of cardiomyocytes derived from human embryonic stem cells (hESCs) to optimize differentiation for potential treatment of myocardial infarction by hESCs.</p><p><strong>Methods: </strong>hESCs were differentiated into cardiomyocytes using standard chemically defined medium 3 (CDM3). Up to day 20 of differentiation, 200 Mm palmitic acid were added, and then the culture was continued for another 8 days to mimic the environment in which human cardiomyocytes mainly use fatty acids as the main energy source. Light microscopy, transmission electron microscopy, immunofluorescence, reverse transcription-polymerase chain reaction, and cellular ATP assays, were carried out to analyze the expression of relevant cardiomyocyte-related genes, cell morphology, metabolism levels, and other indicators cardiomyocyte maturity.</p><p><strong>Results: </strong>Cardiomyocytes derived from hESCs under exogenous palmitic acid had an elongated pike shape and a more regular arrangement. Sarcomere stripes were clear, and the cells color was clearly visible. The cell perimeter and elongation rate were also increased. Myogenic fibers were abundant, myofibrillar z-lines were regularly, the numbers of mitochondria and mitochondrial cristae were higher, more myofilaments were observed, and the structure of round-like discs was occasionally seen. Expression of mature cardiomyocyte-associated genes TNNT2, MYL2 and MYH6, and cardiomyocyte-associated genes KCNJ4, RYR2,and PPARα, was upregulated (<i>p</i> < 0.05). Expression of MYH7, MYL7, KCND2, KCND3, GJA1 and TNNI1 genes was unaffected (<i>p</i> > 0.05). Expression of mature cardiomyocyte-associated sarcomere protein MYL2 was significantly increased (<i>p</i> < 0.05), MYH7 protein expression was unaffected (<i>p</i> > 0.05). hESC-derived cardiomyocytes exposed to exogenous palmitic acid produced more ATP per unit time (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>Exogenous palmitic acid induced more mature hESC-CMs in terms of the cellular architecture, expression of cardiomyocyte maturation genes adnprotein, and metabolism.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"34-42"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142341569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of decellularization strategy and biocompatibility testing of full-thickness abdominal wall to produce a tissue-engineered graft.","authors":"George Skepastianos, Panagiotis Mallis, Epameinondas Kostopoulos, Efstathios Michalopoulos, Vasileios Skepastianos, Christos Doudakmanis, Chrysoula Palazi, Gerasimos Tsourouflis","doi":"10.3233/BME-240144","DOIUrl":"10.3233/BME-240144","url":null,"abstract":"<p><strong>Background: </strong>Restoration of the abdominal wall defects due to herniation or other complications represents a challenging task of the reconstructive surgery. Synthetic grafts or crosslinked animal-derived grafts, are utilized, followed by significant adverse reactions.</p><p><strong>Objective: </strong>This study aimed to evaluate primarily the production of a decellularized abdominal wall scaffold and secondly its biocompatibility upon transplantation in an animal model.</p><p><strong>Methods: </strong>Full-thickness abdominal wall samples were harvested from Wistar Rats and then decellularized utilizing a three-cycle process. To evaluate the decellularization efficacy, histological, biochemical and biomechanical analyses were performed. The biocompatibility assessment involved the implantation of the produced scaffolds to Sprague Dawley rats. The grafts remained for a total period of 4 weeks, followed by immunohistochemistry for the detection of CD11b+, CD4+ and CD8+ cells.</p><p><strong>Results: </strong>Histological, biochemical and biomechanical results, indicated the production of compatible acellular full-thickness abdominal wall samples. After 4 weeks of implantation, a minor presence of immunity cells was observed.</p><p><strong>Conclusion: </strong>The data of this study indicated the successful production of a full-thickness abdominal wall scaffold. Biologically derived full-thickness abdominal wall scaffolds may have greater potential in restoration of the abdominal wall defects, bringing them one step closer to their clinical utility.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"43-53"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142341567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of bioceramic inclusions on gel-cast aliphatic polymer membranes for bone tissue engineering applications: An <i>in vitro</i> study.","authors":"Vasudev Vivekanand Nayak, Edmara T P Bergamo, Vijayavenkataraman Sanjairaj, Rakesh Kumar Behera, Nikhil Gupta, Paulo G Coelho, Lukasz Witek","doi":"10.3233/BME-240079","DOIUrl":"10.3233/BME-240079","url":null,"abstract":"<p><strong>Background: </strong>Polylactic acid (PLA) has been extensively used in tissue engineering. However, poor mechanical properties and low cell affinity have limited its pertinence in load bearing bone tissue regeneration (BTR) devices.</p><p><strong>Objective: </strong>Augmenting PLA with β-Tricalcium Phosphate (β-TCP), a calcium phosphate-based ceramic, could potentially improve its mechanical properties and enhance its osteogenic potential.</p><p><strong>Methods: </strong>Gels of PLA and β-TCP were prepared of different % w/w ratios through polymer dissolution in acetone, after which polymer-ceramic membranes were synthesized using the gel casting workflow and subjected to characterization.</p><p><strong>Results: </strong>Gel-cast polymer-ceramic constructs were associated with significantly higher osteogenic capacity and calcium deposition in differentiated osteoblasts compared to pure polymer counterparts. Immunocytochemistry revealed cell spreading over the gel-cast membrane surfaces, characterized by trapezoidal morphology, distinct rounded nuclei, and well-aligned actin filaments. However, groups with higher ceramic loading expressed significantly higher levels of osteogenic markers relative to pure PLA membranes. Rule of mixtures and finite element models indicated an increase in theoretical mechanical strength with an increase in β-TCP concentration.</p><p><strong>Conclusion: </strong>This study potentiates the use of PLA/β-TCP composites in load bearing BTR applications and the ability to be used as customized patient-specific shape memory membranes in guided bone regeneration.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"15-33"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142341568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis of gold nanoparticles and their protective effect towards diabetic nephropathy by inhibition of oxidative stress.","authors":"Bo Qu, Yuhua He, Shanlin Liu, Yulian Huang, Yingchun Bai","doi":"10.1177/09592989241296432","DOIUrl":"https://doi.org/10.1177/09592989241296432","url":null,"abstract":"<p><strong>Background: </strong>Diabetes is an emerging health issue on a global scale, with increasing prevalence rates reported in many countries. Many mechanisms are proposed for diabetic nephropathy, with oxidative stress being the most significant. The effectiveness of gold nanoparticles (AuNPs) in the attenuation of nephropathy and oxidative stress in diabetic mice was assessed in this study.</p><p><strong>Objective: </strong>The aim of this study is to synthesize AuNPs and assess their protective effect towards diabetic nephropathy by inhibition of oxidative stress.</p><p><strong>Methods: </strong>The aqueous extract of <i>Allium sativum</i> was employed to synthesize AuNPs. The prepared AuNPs were characterized using a variety of microscopic and spectroscopic techniques. <i>In-vitro</i> studies were conducted using mice. Streptozotocin (STZ) was employed to induce diabetes in rodents. After 7 days of administration of STZ, anesthesia was given to all animals and blood was collected for the assessment of creatinine and Blood Urea Nitrogen (BUN) levels. Later, kidney tissue was removed at 4 °C and changes in pathology and oxidative stress were assessed.</p><p><strong>Results: </strong>Nephropathy was confirmed in diabetic mice by the changes in the pathology of kidney tissue along with significant rise in the plasma levels of BUN and creatinine. Additionally, the peroxidation of lipids, formation of Reactive Oxygen Species (ROS), oxidation of glutathione (GSH), concentration of carbonyl protein was also increased in the tissue of kidney of diabetic mice. Oxidative stress in kidney tissue and changes in the pathology of diabetic mice were inhibited significantly (p < 0.05) with the treatment of AuNPs.</p><p><strong>Conclusion: </strong>This study revealed the protective effects of AuNPs over diabetic nephropathy by inhibiting the pathway of oxidative stress. Since, the prepared AuNPs showed improvement over complications of diabetes, they may be believed as a potential gratuitous treatment next to other drugs for reducing blood glucose.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"36 1","pages":"54-66"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}