Bio-medical materials and engineering最新文献

{"title":"Numerical simulation of hemodynamics in patient-specific pulmonary artery stenosis.","authors":"Fan He, Xinyu Wang, Lu Hua, Tingting Guo","doi":"10.3233/BME-222523","DOIUrl":"https://doi.org/10.3233/BME-222523","url":null,"abstract":"BACKGROUND The incidence rate of pulmonary artery stenosis is increasing year by year and its numerical simulation has become a key project of biomedical engineering. OBJECTIVE The purpose of this work is to study the changes of hemodynamic parameters in patient-specific pulmonary artery stenosis. METHODS A pulmonary artery stenosis model is established based on patient-specific computed tomography (CT) images. According to the actual anatomy of patient-specific pulmonary artery stenosis, the stenosis area is simulated using a porous medium to study its hemodynamic changes. The computational fluid dynamics (CFD) method is used to simulate the hemodynamic changes of pulmonary artery stenosis, and to explore the mechanical characteristics between blood flow and vessel wall. RESULTS The results suggest that the blood pressure of arterial branches increases and the pressure drop at both ends of the stenosis is higher. There is a high flow rate and wall shear stress at the stenosis. CONCLUSION This study shows that the hemodynamic model of pulmonary artery stenosis can be accurately reconstructed by achieving numerical simulation of the local stenosis through CT images, and this work has important implications for improving the confidence of clinical diagnosis and treatment of pulmonary artery diseases.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"34 5","pages":"427-437"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10300561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunological characteristics of human umbilical cord mesenchymal stem cells after hepatogenic differentiation.","authors":"Yanfeng Yin,&nbsp;Zongqiang Hu,&nbsp;Zheng Guan,&nbsp;Sha Lv,&nbsp;Yiyin Wang,&nbsp;Wenjun Su,&nbsp;Chuntao Yan,&nbsp;Yiting Wang,&nbsp;Li Ma,&nbsp;Jie Jiang,&nbsp;Shiming Feng,&nbsp;Li Li","doi":"10.3233/BME-211331","DOIUrl":"https://doi.org/10.3233/BME-211331","url":null,"abstract":"<p><strong>Background: </strong>Acute liver failure is one of the most intractable clinical problems. The use of bioartificial livers may solve donor shortage problems. Human umbilical cord mesenchymal stem cells (hUCMSCs) are an excellent seed cell choice for artificial livers because they change their characteristics to resemble hepatocyte-like cells (HLCs) following artificial liver transplantation.</p><p><strong>Objective: </strong>This study aimed to determine whether the immunological characteristics of hUCMSCs are changed after being transformed into hepatocyte-like cells.</p><p><strong>Methods: </strong>HUCMSCs were isolated by the adherent method. The following hUCMSC surface markers were detected using flow cytometry: CD45, CD90, CD105, CD34, and octamer-binding transcription factor 4 (OCT-4). Functional detection of adipogenic differentiation was performed. The hUCMSCs were cultured in complete medium (control group) or induction medium (induction group), and flow cytometry was used to detect cell surface markers. Peritoneal lavage fluid was collected after intraperitoneal injection of 1 × 106 cells/mouse over 40 minutes. The leukocyte count, labeled CD45, CD3, CD4 and CD8 antibodies, and flow detection of T lymphocyte subsets were determined using the peritoneal lavage fluid.</p><p><strong>Results: </strong>Using phenotypic and functional identification, hUCMSCs were successfully isolated using a two-step induction method. The surface markers of the hUCMSCs cells changed after HLC induction. In vivo immune results showed that hUCMSCs and HLsC induced leukocyte production.</p><p><strong>Conclusion: </strong>Hepatic induction of hUCMSCs changes their cell surface markers. Both HLCs and hUCMSCs cause leukocytosis in vivo, but the immune response induced by HLCs is slightly stronger.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"34 1","pages":"1-11"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10563212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virtual reality exposure aided-diagnosis system for anxiety disorders: Long short-term memory architecture for three levels of anxiety recognition.","authors":"Wahida Handouzi, Choubeila Maaoui, Alain Pruski","doi":"10.3233/BME-222542","DOIUrl":"10.3233/BME-222542","url":null,"abstract":"<p><strong>Background: </strong>The COVID-19 pandemic has resulted in increased psychological pressure on mental health since 2019. The resulting anxiety and stress have permeated every aspect of life during confinement.</p><p><strong>Objective: </strong>To provide psychologists with an unbiased measure that can aid in the preliminary diagnosis of anxiety disorders and be used as an initial treatment in cognitive-behavioral therapy, this article introduces automated recognition of three levels of anxiety.</p><p><strong>Methods: </strong>Anxiety was elicited by exposing participants to virtual environments inspired by social situations in reference to the Liebowitz social anxiety scale. Relevant parameters, such as heart rate variability and vasoconstriction were derived from the measurement of the blood volume pulse (BVP) signal.</p><p><strong>Results: </strong>A long short-term memory architecture achieved an accuracy of approximately 98% on the training and test set.</p><p><strong>Conclusion: </strong>The generated model allowed for careful study of the state of seven phobic participants during virtual reality exposure (VRE).</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"491-502"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9911792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of photodynamic therapy on the marginal adaptation of Biodentine used as root-end filling material.","authors":"Ivanka Dimitrova, Yaneta Kouzmanova","doi":"10.3233/BME-221469","DOIUrl":"10.3233/BME-221469","url":null,"abstract":"<p><strong>Background: </strong>The marginal adaptation of root-end filling materials and the effective antibacterial control in a surgical site are crucial for the successful outcome of endodontic surgery.</p><p><strong>Objective: </strong>This study aimed to evaluate the effect of retrograde application of photodynamic therapy on the marginal adaptation of Biodentine used as a root-end filling material.</p><p><strong>Methods: </strong>Twenty single-rooted anterior teeth were selected, instrumented and obturated with gutta-percha and AH Plus. The apical 3 mm of the roots were resected and root-end cavities were prepared with an ultrasonic retro-tip. The teeth were randomly divided into two groups (n = 10). In the first group, photodynamic therapy (PDT) was applied in the retrograde cavity prior to the root-end filling. In the second group retro cavity was filled without PDT. All specimens were obturated with Biodentine and afterwards sectioned longitudinally. The gap width at the material-dentin interface was measured using a scanning electron microscope. The results were statistically analyzed.</p><p><strong>Results: </strong>The produced gap width by Biodentine/PDT was 3.85 μm versus 2.68 μm in the Biodentine control group with significant differences in-between.</p><p><strong>Conclusion: </strong>Under the conditions of this study, PDT has a negative effect on the marginal adaptation of Biodentine used as root-end filling material.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"34 3","pages":"277-287"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9493272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of shear stress related hemolysis in a ventricular assist device.","authors":"Mohamed Bounouib,&nbsp;Hind Benakrach,&nbsp;Mourad Taha-Janan,&nbsp;Wajih Maazouzi","doi":"10.3233/BME-221401","DOIUrl":"https://doi.org/10.3233/BME-221401","url":null,"abstract":"<p><strong>Background: </strong>Implantable devices such as ventricular assist devices provide appropriate treatment for patients with advanced heart failure. Unfortunately, these devices still have many problems, particularly related to blood damage.</p><p><strong>Objective: </strong>The aim of this research is to examine two new ventricular assist devices in terms of induced shear stress, exposure time, and induced hemolysis.</p><p><strong>Method: </strong>Reverse engineering was used on multiple axial flow ventricular assist devices to collect all the details related to the designs (diameters, lengths, blade angles…), which were used to build two prototypes: Model A and Model B.</p><p><strong>Results: </strong>The obtained results were close to a large extent, except for static pressure rise, where the difference was clear.</p><p><strong>Conclusion: </strong>Compared with what has been published in other studies, the overall performance of both models was excellent.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"34 1","pages":"51-66"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9135891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo degradation and bone reaction of long-term fixation with a magnesium alloy made by twin-roll casting in a rat femur model.","authors":"Ying Zhang,&nbsp;Haijian Wang,&nbsp;Takashi Kumazawa,&nbsp;Dongying Ju","doi":"10.3233/BME-221415","DOIUrl":"https://doi.org/10.3233/BME-221415","url":null,"abstract":"<p><strong>Background: </strong>The effect of casting parameters on the microstructure and corrosion resistance of Mg alloys is still limited, especially in clinical animal experiments.</p><p><strong>Objective: </strong>We prepared a new magnesium rare earth alloy (Mg-Re, where Re is Ce or La) by vertical two-roll casting and Mg-A by further rolling. The microstructure characteristics, degradation behavior, and bone reaction of the two alloys were studied.</p><p><strong>Method: </strong>Ti, Mg-Re, and Mg-A alloy plates were implanted in a rat femur model, and their degradation behavior was observed 48 weeks later.</p><p><strong>Results: </strong>In vivo experiments showed no significant changes around the femur in the Ti group, excluding external factors that may cause bone remodeling and lead to new bone formation. Mg-A induces more new bone formation than Mg-Re, which meets the necessary conditions to prevent pathological fracture. The specimen staining and sectioning showed that the liver and heart of rats implanted with magnesium alloys had no pathological changes and the cell structure was normal, similar to that of rats without a magnesium alloy.</p><p><strong>Conclusion: </strong>Mg-A alloy has good healing potential as a biodegradable implant material.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"34 2","pages":"169-181"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9095728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of super activated platelet lysate on cell proliferation, repair and osteogenesis.","authors":"Xiaorui Guo,&nbsp;Chunxiang Liu,&nbsp;Yi Zhang,&nbsp;Liangjia Bi","doi":"10.3233/BME-221426","DOIUrl":"https://doi.org/10.3233/BME-221426","url":null,"abstract":"BACKGROUND Platelet lysate (PL) is considered as an alternative to fetal bovine serum (FBS) and facilitates the proliferation and differentiation of mesenchymal cells. OBJECTIVE The aim of this study is to explore whether super activated platelet lysate (sPL), a novel autologous platelet lysate, has the ability to inhibit inflammation and promote cell proliferation, repair and osteogenesis as a culture medium. METHODS Different concentrations of sPL on human fetal osteoblastic 1.19 cell line (hFOB1.19) proliferation and apoptotic repair were investigated; And detected proliferative capacity, inflammatory factor expressions and osteogenic differentiation of human dental pulp cells (hDPCs) stimulated by LPS under 10% FBS and 5% sPL mediums. RESULTS sPL promoted hFOB1.19 proliferation and had repairing effects on apoptotic cells. No significant difference in proliferation and IL-1α, IL-6 and TNF-α expressions of hDPCs in FBS and sPL medium stimulated by LPS. hDPCs in sPL osteogenic medium had higher osteogenic-related factor expressions and ALP activity. LPS promoted osteogenic-related factor expressions and ALP activity of hDPCs in FBS osteogenic medium, but opposite effect showed in sPL medium. CONCLUSION sPL promoted osteoblast proliferation and had restorative effects. Under LPS stimulation, sPL did not promote hDPCs proliferation or inhibit inflammation. sPL promotes osteogenic differentiation of hDPCs.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"34 1","pages":"95-109"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10570673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of biocompatibility and angiogenic potential of extracellular matrix hydrogel biofunctionalized with the LL-37 peptide.","authors":"Jorge López-Gutierrez, Rosalío Ramos-Payán, Jose Geovanni Romero-Quintana, Alfredo Ayala-Ham, Yolanda Castro-Salazar, Hipolito Castillo-Ureta, German Jiménez-Gastélum, Mercedes Bermúdez, Maribel Aguilar-Medina","doi":"10.3233/BME-230022","DOIUrl":"10.3233/BME-230022","url":null,"abstract":"<p><strong>Background: </strong>Biomaterials must allow revascularization for a successful tissue regeneration process. Biomaterials formulated from the extracellular matrix (ECM) have gained popularity in tissue engineering because of their superior biocompatibility, and due to their rheological properties, ECM-hydrogels can be easily applied in damaged areas, allowing cell colonization and integration into the host tissue. Porcine urinary bladder ECM (pUBM) retains functional signaling and structural proteins, being an excellent option in regenerative medicine. Even some small molecules, such as the antimicrobial cathelicidin-derived LL-37 peptide have proven angiogenic properties.</p><p><strong>Objective: </strong>The objective of this study was to evaluate the biocompatibility and angiogenic potential of an ECM-hydrogel derived from the porcine urinary bladder (pUBMh) biofunctionalized with the LL-37 peptide (pUBMh/LL37).</p><p><strong>Methods: </strong>Macrophages, fibroblasts, and adipose tissue-derived mesenchymal stem cells (AD-MSC) were exposed pUBMh/LL37, and the effect on cell proliferation was evaluated by MTT assay, cytotoxicity by quantification of lactate dehydrogenase release and the Live/Dead Cell Imaging assays. Moreover, macrophage production of IL-6, IL-10, IL-12p70, MCP-1, INF-γ, and TNF-α cytokines was quantified using a bead-based cytometric array. pUBMh/LL37 was implanted directly by dorsal subcutaneous injection in Wistar rats for 24 h to evaluate biocompatibility, and pUBMh/LL37-loaded angioreactors were implanted for 21 days for evaluation of angiogenesis.</p><p><strong>Results: </strong>We found that pUBMh/LL37 did not affect cell proliferation and is cytocompatible to all tested cell lines but induces the production of TNF-α and MCP-1 in macrophages. In vivo, this ECM-hydrogel induces fibroblast-like cell recruitment within the material, without tissue damage or inflammation at 48 h. Interestingly, tissue remodeling with vasculature inside angioreactors was seen at 21 days.</p><p><strong>Conclusions: </strong>Our results showed that pUBMh/LL37 is cytologically compatible, and induces angiogenesis in vivo, showing potential for tissue regeneration therapies.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"545-560"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9728484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D printed mesoporous bioactive glass, bioglass 45S5, and β-TCP scaffolds for regenerative medicine: A comparative in vitro study.","authors":"Maria Jesus Pacheco-Vergara,&nbsp;John L Ricci,&nbsp;Dindo Mijares,&nbsp;Timothy G Bromage,&nbsp;Sasan Rabieh,&nbsp;Paulo G Coelho,&nbsp;Lukasz Witek","doi":"10.3233/BME-222524","DOIUrl":"https://doi.org/10.3233/BME-222524","url":null,"abstract":"<p><strong>Background: </strong>While autografts to date remain the \"gold standard\" for bone void fillers, synthetic bone grafts have garnered attention due to their favorable advantages such as ability to be tailored in terms of their physical and chemical properties. Bioactive glass (BG), an inorganic material, has the capacity to form a strong bond with bone by forming a bone-like apatite surface, enhancing osteogenesis. Coupled with additive manufacturing (3D printing) it is possible to maximize bone regenerative properties of the BG.</p><p><strong>Objective: </strong>The objective of this study was to synthesize and characterize 3D printed mesoporous bioactive glass (MBG), BG 45S5, and compare to β-Tricalcium phosphate (β-TCP) based scaffolds; test cell viability and osteogenic differentiation on human osteoprogenitor cells in vitro.</p><p><strong>Methods: </strong>MBG, BG 45S5, and β-TCP were fabricated into colloidal gel suspensions, tested with a rheometer, and manufactured into scaffolds using a 3D direct-write micro-printer. The materials were characterized in terms of microstructure and composition with Thermogravimetric Analyzer/Differential Scanning Calorimeter (TGA/DSC), Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Micro-Computed Tomography (μ-CT), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDS), and Mattauch-Herzog-Inductively Coupled Plasma-Mass Spectrometry (MH-ICP-MS).</p><p><strong>Results: </strong>Scaffolds were tested for cell proliferation and osteogenic differentiation using human osteoprogenitor cells. Osteogenic media was used for differentiation, and immunocytochemistry for osteogenic markers Runx-2, Collagen-I, and Osteocalcin. The cell viability results after 7 days of culture yielded significantly higher (p < 0.05) results in β-TCP scaffolds compared to BG 45S5 and MBG groups.</p><p><strong>Conclusion: </strong>All materials expressed osteogenic markers after 21 days of culture in expansion and osteogenic media.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"34 5","pages":"439-458"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10294336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro evaluation of crosslinked bovine pericardium as potential scaffold for the oral cavity.","authors":"Guadalupe Del Carmén Ordóñez-Chávez, Nayeli Rodríguez-Fuentes, Ricardo Peñaloza-Cuevas, José Manuel Cervantes-Uc, Luz Eugenia Alcántara-Quintana, Ixchel Araceli Maya-García, Virginia Aurora Herrera-Valencia, Celia Elena Mendiburu-Zavala","doi":"10.3233/BME-230027","DOIUrl":"10.3233/BME-230027","url":null,"abstract":"<p><strong>Background: </strong>Bovine pericardium (BP) is a scaffold widely used in soft tissues regeneration; however, its calcification in contact with glutaraldehyde, represent an opportunity for its application in hard tissues, such as bone in the oral cavity.</p><p><strong>Objective: </strong>To develop and to characterize decellularized and glutaraldehyde-crosslinked bovine pericardium (GC-BP) as a potential scaffold for guided bone regeneration GBR.</p><p><strong>Methods: </strong>BP samples from healthy animals of the bovine zebu breed were decellularized and crosslinked by digestion with detergents and glutaraldehyde respectively. The resulting cell-free scaffold was physical, chemical, mechanical, and biologically characterized thought hematoxylin and eosin staining, DNA quantification, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), uniaxial tensile test, cell viability and live and dead assay in cultures of dental pulp stem cells (DPSCs).</p><p><strong>Results: </strong>The decellularization and crosslinking of BP appeared to induce conformational changes of the CLG molecules, which led to lower mechanical properties at the GC-BP scaffold, at the same time that promoted cell adhesion and viability of DPSCs.</p><p><strong>Conclusion: </strong>This study suggests that the decellularized and GC-BP is a scaffold with the potential to be used promoting DPSCs recruitment, which has a great impact on the dental area.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"561-575"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10657658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10302842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}