In situ-formed hyaluronan gel/BMP-2/hydroxyapatite composite promotes bone union in refractory fracture model mice.

IF 1 4区医学 Q4 ENGINEERING, BIOMEDICAL

Bio-medical materials and engineering Pub Date : 2023-01-01 DOI:10.3233/BME-230021

Hiroki Saito, Shintaro Shoji, Akiyoshi Kuroda, Gen Inoue, Ryo Tazawa, Hiroyuki Sekiguchi, Kensuke Fukushima, Masayuki Miyagi, Masashi Takaso, Kentaro Uchida

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引用次数: 0

Abstract

Background: A combination of synthetic porous materials and BMP-2 has been used to promote fracture healing. For bone healing to be successful, it is important to use growth factor delivery systems that enable continuous release of BMP-2 at the fracture site. We previously reported that in situ-formed gels (IFGs) consisting of hyaluronan (HyA)-tyramine (TA), horseradish peroxidase and hydrogen peroxide enhance the bone formation ability of hydroxyapatite (Hap)/BMP-2 composites in a posterior lumbar fusion model.

Objective: We examined the effectiveness of IFGs-HyA/Hap/BMP-2 composites for facilitating osteogenesis in refractory fracture model mice.

Methods: After establishing the refractory fracture model, animals were either treated at the site of fracture with Hap harboring BMP-2 (Hap/BMP-2) or IFGs-HyA with Hap harboring BMP-2 (IFGs-HyA/Hap/BMP-2) (n = 10 each). Animals that underwent the fracture surgery but did not receive any treatment were considered the control group (n = 10). We determined the extent of bone formation at the fracture site according to findings on micro-computed tomography and histological studies four weeks following treatment.

Results: Animals treated with IFGs-HyA/Hap/BMP-2 demonstrated significantly greater bone volume, bone mineral content and bone union than those treated with vehicle or IFG-HyA/Hap alone.

Conclusions: IFGs-HyA/Hap/BMP-2 could be an effective treatment option for refractory fractures.

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原位形成的透明质酸凝胶/BMP-2/羟基磷灰石复合物促进难治性骨折模型小鼠骨愈合。

背景:合成多孔材料与BMP-2的结合已被用于促进骨折愈合。为了使骨愈合成功，重要的是使用生长因子输送系统，使BMP-2在骨折部位持续释放。我们之前报道过由透明质酸(HyA)-酪胺(TA)、辣根过氧化物酶和过氧化氢组成的原位形成凝胶(IFGs)增强了后路腰椎融合模型中羟基磷灰石(Hap)/BMP-2复合材料的成骨能力。目的:观察IFGs-HyA/Hap/BMP-2复合材料对难治性骨折模型小鼠成骨的促进作用。方法:建立难治性骨折模型后，分别在骨折部位植入Hap - BMP-2 (Hap/BMP-2)或IFGs-HyA植入Hap - BMP-2 (IFGs-HyA/Hap/BMP-2) (n = 10)。接受骨折手术但未接受任何治疗的动物被视为对照组(n = 10)。我们根据治疗后四周的显微计算机断层扫描和组织学研究结果确定骨折部位骨形成的程度。结果:IFGs-HyA/Hap/BMP-2处理动物的骨体积、骨矿物质含量和骨愈合均显著高于载体或IFG-HyA/Hap单独处理动物。结论:IFGs-HyA/Hap/BMP-2可能是治疗难治性骨折的有效选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bio-medical materials and engineering 工程技术-材料科学：生物材料

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： The aim of Bio-Medical Materials and Engineering is to promote the welfare of humans and to help them keep healthy. This international journal is an interdisciplinary journal that publishes original research papers, review articles and brief notes on materials and engineering for biological and medical systems. Articles in this peer-reviewed journal cover a wide range of topics, including, but not limited to: Engineering as applied to improving diagnosis, therapy, and prevention of disease and injury, and better substitutes for damaged or disabled human organs; Studies of biomaterial interactions with the human body, bio-compatibility, interfacial and interaction problems; Biomechanical behavior under biological and/or medical conditions; Mechanical and biological properties of membrane biomaterials; Cellular and tissue engineering, physiological, biophysical, biochemical bioengineering aspects; Implant failure fields and degradation of implants. Biomimetics engineering and materials including system analysis as supporter for aged people and as rehabilitation; Bioengineering and materials technology as applied to the decontamination against environmental problems; Biosensors, bioreactors, bioprocess instrumentation and control system; Application to food engineering; Standardization problems on biomaterials and related products; Assessment of reliability and safety of biomedical materials and man-machine systems; and Product liability of biomaterials and related products.