Effect of ellagic acid and retinoic acid on collagen and elastin production by human dermal fibroblasts.

IF 1 4区医学 Q4 ENGINEERING, BIOMEDICAL

Bio-medical materials and engineering Pub Date : 2023-01-01 DOI:10.3233/BME-230007

Chloe Duckworth, Jada Stutts, Kayla Clatterbuck, Nasim Nosoudi

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引用次数: 1

Abstract

Background: Elastin is a fibrous protein key to the structure and support of skin as well as other organ tissues. Elastic fibers are located in the skin's dermal layer and make up approximately 2%-4% of the fat-free dry weight of the dermis in the skin of adults. Aging causes the progressive degradation of elastin fibers. Loss of these fibers can cause skin sagging and wrinkling, loss of healthy blood vessels and lung capacity, aneurysms, and Chronic Obstructive Pulmonary Disease (COPD).

Objective: We hypothesized that ellagic acid, a polyphenol, will increase elastin in human dermal fibroblasts (HDF) due to polyphenols' elastin binding properties.

Method: We treated HDF's with 2 μg/ml ellagic acid for 28 days to see the elastin deposition in HDF cell cultures. To test this, we treated HDFs with polyphenols ellagic acid for 3, 7, 14 and 21 days. For comparison purposes, we included a group of ellagic acid and retinoic acid since retinoic acid is already in the market for elastin regeneration purposes.

Results: When ellagic acid and retinoic acid were introduced together, insoluble elastin and collagen deposition were significantly higher in HDFs compared to other groups.

Conclusion: Polyphenols and retinoic acid can improve skin extracellular matrix production of elastin and collagen and may improve skin fine wrinkles.

查看原文本刊更多论文

鞣花酸和维甲酸对人真皮成纤维细胞生成胶原和弹性蛋白的影响。

背景:弹性蛋白是一种纤维蛋白，对皮肤和其他器官组织的结构和支持至关重要。弹性纤维位于皮肤的真皮层，约占成人皮肤真皮层无脂肪干重的2%-4%。老化导致弹性蛋白纤维的逐渐退化。这些纤维的丢失会导致皮肤松弛和起皱，健康血管和肺活量的丧失，动脉瘤和慢性阻塞性肺疾病(COPD)。目的:我们假设鞣花酸，一种多酚，会增加人真皮成纤维细胞(HDF)的弹性蛋白，由于多酚的弹性蛋白结合特性。方法:用2 μg/ml鞣花酸处理HDF细胞28 d，观察弹性蛋白在HDF细胞中的沉积情况。为了验证这一点，我们用鞣花酸多酚处理HDFs 3、7、14和21天。为了比较，我们纳入了一组鞣花酸和视黄酸，因为视黄酸已经在市场上用于弹性蛋白再生。结果:当鞣花酸和维甲酸同时加入时，HDFs中不溶性弹性蛋白和胶原沉积明显高于其他组。结论:多酚类和维甲酸能促进皮肤细胞外基质弹性蛋白和胶原蛋白的生成，改善皮肤细纹。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bio-medical materials and engineering 工程技术-材料科学：生物材料

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： The aim of Bio-Medical Materials and Engineering is to promote the welfare of humans and to help them keep healthy. This international journal is an interdisciplinary journal that publishes original research papers, review articles and brief notes on materials and engineering for biological and medical systems. Articles in this peer-reviewed journal cover a wide range of topics, including, but not limited to: Engineering as applied to improving diagnosis, therapy, and prevention of disease and injury, and better substitutes for damaged or disabled human organs; Studies of biomaterial interactions with the human body, bio-compatibility, interfacial and interaction problems; Biomechanical behavior under biological and/or medical conditions; Mechanical and biological properties of membrane biomaterials; Cellular and tissue engineering, physiological, biophysical, biochemical bioengineering aspects; Implant failure fields and degradation of implants. Biomimetics engineering and materials including system analysis as supporter for aged people and as rehabilitation; Bioengineering and materials technology as applied to the decontamination against environmental problems; Biosensors, bioreactors, bioprocess instrumentation and control system; Application to food engineering; Standardization problems on biomaterials and related products; Assessment of reliability and safety of biomedical materials and man-machine systems; and Product liability of biomaterials and related products.