{"title":"BMP-2促进小鼠磷酸钙基复合材料的新骨形成。","authors":"Liuxing He, Xue Wang, Nana Xiong, Zhihong Dong, Lijia Cheng","doi":"10.1177/09592989251346468","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Autologous bone grafting, particularly using iliac crest bone grafts, historically been considered the gold standard for bone grafting. However, its limited availability and secondary surgical trauma associated with the procedure have restricted its widespread application.</p><p><strong>Objective: </strong>To investigate the osteogenic potential of calcium phosphate-based composites with or without the addition of bone morphogenetic protein-2 (BMP-2).</p><p><strong>Methods: </strong>The BMP-2/calcium phosphate (CaP)/temperature-sensitive hydrogel (BCT) composite was synthesized, while the CaP/temperature-sensitive hydrogel (CT) composite was prepared as control group. The surface morphology of the composites was observed using scanning electron microscope (SEM). Subsequently, the composites were co-cultured with mouse bone marrow mesenchymal stem cells (BMSCs), and the cell proliferation, alkaline phosphatase (ALP) activity and extracellular matrix (ECM) mineralization were assessed. The composites were injected into the muscle of mice, and the samples underwent hematoxylin and eosin (HE), Masson-trichrome, and safranin and fast green staining. Immunohistochemistry for BMP-2 and type I collagen (ColI) was performed at weeks 8 and 12.</p><p><strong>Results: </strong>There was no significant difference in cell proliferation between the BCT and CT groups. However, the relative ALP activity and ECM mineralization were significantly higher in BCT group compared to the CT group (<i>P</i> < 0.05). BMP-2 accelerated the osteoinduction process and promoted the formation of more new bone tissue and bone marrow in group BCT. The number of osteocytes and the collagen area ratio were significantly higher in group BCT than that in group CT (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>BMP-2 and CaP synergistically accelerated the initiation of osteoinduction, and promoted increased bone formation. The temperature-sensitive hydrogel made the material injectable, expanding its range of applications. The BCT composite shows promise as an artificial bone material.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"9592989251346468"},"PeriodicalIF":1.0000,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"BMP-2 enhanced new bone formation of calcium phosphate-based composites in mice.\",\"authors\":\"Liuxing He, Xue Wang, Nana Xiong, Zhihong Dong, Lijia Cheng\",\"doi\":\"10.1177/09592989251346468\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Autologous bone grafting, particularly using iliac crest bone grafts, historically been considered the gold standard for bone grafting. However, its limited availability and secondary surgical trauma associated with the procedure have restricted its widespread application.</p><p><strong>Objective: </strong>To investigate the osteogenic potential of calcium phosphate-based composites with or without the addition of bone morphogenetic protein-2 (BMP-2).</p><p><strong>Methods: </strong>The BMP-2/calcium phosphate (CaP)/temperature-sensitive hydrogel (BCT) composite was synthesized, while the CaP/temperature-sensitive hydrogel (CT) composite was prepared as control group. The surface morphology of the composites was observed using scanning electron microscope (SEM). Subsequently, the composites were co-cultured with mouse bone marrow mesenchymal stem cells (BMSCs), and the cell proliferation, alkaline phosphatase (ALP) activity and extracellular matrix (ECM) mineralization were assessed. The composites were injected into the muscle of mice, and the samples underwent hematoxylin and eosin (HE), Masson-trichrome, and safranin and fast green staining. Immunohistochemistry for BMP-2 and type I collagen (ColI) was performed at weeks 8 and 12.</p><p><strong>Results: </strong>There was no significant difference in cell proliferation between the BCT and CT groups. However, the relative ALP activity and ECM mineralization were significantly higher in BCT group compared to the CT group (<i>P</i> < 0.05). BMP-2 accelerated the osteoinduction process and promoted the formation of more new bone tissue and bone marrow in group BCT. The number of osteocytes and the collagen area ratio were significantly higher in group BCT than that in group CT (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>BMP-2 and CaP synergistically accelerated the initiation of osteoinduction, and promoted increased bone formation. The temperature-sensitive hydrogel made the material injectable, expanding its range of applications. The BCT composite shows promise as an artificial bone material.</p>\",\"PeriodicalId\":9109,\"journal\":{\"name\":\"Bio-medical materials and engineering\",\"volume\":\" \",\"pages\":\"9592989251346468\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2025-06-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bio-medical materials and engineering\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1177/09592989251346468\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bio-medical materials and engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1177/09592989251346468","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
BMP-2 enhanced new bone formation of calcium phosphate-based composites in mice.
Background: Autologous bone grafting, particularly using iliac crest bone grafts, historically been considered the gold standard for bone grafting. However, its limited availability and secondary surgical trauma associated with the procedure have restricted its widespread application.
Objective: To investigate the osteogenic potential of calcium phosphate-based composites with or without the addition of bone morphogenetic protein-2 (BMP-2).
Methods: The BMP-2/calcium phosphate (CaP)/temperature-sensitive hydrogel (BCT) composite was synthesized, while the CaP/temperature-sensitive hydrogel (CT) composite was prepared as control group. The surface morphology of the composites was observed using scanning electron microscope (SEM). Subsequently, the composites were co-cultured with mouse bone marrow mesenchymal stem cells (BMSCs), and the cell proliferation, alkaline phosphatase (ALP) activity and extracellular matrix (ECM) mineralization were assessed. The composites were injected into the muscle of mice, and the samples underwent hematoxylin and eosin (HE), Masson-trichrome, and safranin and fast green staining. Immunohistochemistry for BMP-2 and type I collagen (ColI) was performed at weeks 8 and 12.
Results: There was no significant difference in cell proliferation between the BCT and CT groups. However, the relative ALP activity and ECM mineralization were significantly higher in BCT group compared to the CT group (P < 0.05). BMP-2 accelerated the osteoinduction process and promoted the formation of more new bone tissue and bone marrow in group BCT. The number of osteocytes and the collagen area ratio were significantly higher in group BCT than that in group CT (P < 0.05).
Conclusion: BMP-2 and CaP synergistically accelerated the initiation of osteoinduction, and promoted increased bone formation. The temperature-sensitive hydrogel made the material injectable, expanding its range of applications. The BCT composite shows promise as an artificial bone material.
期刊介绍：
The aim of Bio-Medical Materials and Engineering is to promote the welfare of humans and to help them keep healthy. This international journal is an interdisciplinary journal that publishes original research papers, review articles and brief notes on materials and engineering for biological and medical systems. Articles in this peer-reviewed journal cover a wide range of topics, including, but not limited to: Engineering as applied to improving diagnosis, therapy, and prevention of disease and injury, and better substitutes for damaged or disabled human organs; Studies of biomaterial interactions with the human body, bio-compatibility, interfacial and interaction problems; Biomechanical behavior under biological and/or medical conditions; Mechanical and biological properties of membrane biomaterials; Cellular and tissue engineering, physiological, biophysical, biochemical bioengineering aspects; Implant failure fields and degradation of implants. Biomimetics engineering and materials including system analysis as supporter for aged people and as rehabilitation; Bioengineering and materials technology as applied to the decontamination against environmental problems; Biosensors, bioreactors, bioprocess instrumentation and control system; Application to food engineering; Standardization problems on biomaterials and related products; Assessment of reliability and safety of biomedical materials and man-machine systems; and Product liability of biomaterials and related products.
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