Brazilian Journal of Medical and Biological Research最新文献_第2页

Systemic effects of oral tolerance improve the healing of several and concomitant wounds on different parts of the body. 口服耐受性的全身性作用可改善身体不同部位的几种和伴随性伤口的愈合。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-04-14 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2025e14689

I B C Nobrega, A V S Andrade, T J N Bikat, G M Quintão, G M Azevedo Junior, K Franco-Valência, R A Costa, C R Carvalho

{"title":"Systemic effects of oral tolerance improve the healing of several and concomitant wounds on different parts of the body.","authors":"I B C Nobrega, A V S Andrade, T J N Bikat, G M Quintão, G M Azevedo Junior, K Franco-Valência, R A Costa, C R Carvalho","doi":"10.1590/1414-431X2025e14689","DOIUrl":"https://doi.org/10.1590/1414-431X2025e14689","url":null,"abstract":"Oral tolerance is an immunological phenomenon that results from protein intake and that has systemic effects on inflammation. Previous research has shown that parenteral injection of tolerated proteins reduces inflammatory infiltrate and improves skin wound healing. Herein, we tested whether the injection of tolerated proteins improves the healing of several wounds on different parts of the body, such as on the skin of the back and on the external ear (the auricle). To induce oral tolerance to ovalbumin (OVA), eight-week old C57BL/6 mice drank egg white diluted 1:5 in water for 3 consecutive days. The control mice drank water. Seven days after oral treatment, mice were submitted to excisional injuries on the skin of the back (6 mm) and ears (4 mm). Minutes before the injuries, the mice received an intraperitoneal injection of OVA + Al(OH)3. Seven and 40 days after the injuries, tissue samples were collected and processed for histological analysis of the wounds. The results showed that the injection of OVA in animals that drank OVA reduced the inflammatory infiltrate in all lesions. In addition, injection of OVA in animals that drank OVA promoted better organization of the extracellular matrix, with thicker and intertwined collagen fibers in the neodermis, resulting in smaller scars on the skin. Furthermore, the healing area of the ears of OVA-tolerant animals showed chondrocyte aggregates and less obvious fibrous scar tissue compared with control animals. In conclusion, systemic effects of oral tolerance positively influenced the healing of several lesions on different parts of the body.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e14689"},"PeriodicalIF":1.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11996163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liraglutide improves antioxidant defense in hearts of spontaneously hypertensive female rats independently of changes in blood pressure in a pre-clinical model of menopause. 利拉鲁肽改善自发性高血压雌性大鼠心脏的抗氧化防御，独立于更年期血压变化的临床前模型。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-04-14 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2025e14209

W C Dos Santos, S N Ronchi, L A Gonçalves, L C S L Oliveira, G J Sousa, A F Melo Junior, T U de Andrade, N S Bissoli, G A Brasil

{"title":"Liraglutide improves antioxidant defense in hearts of spontaneously hypertensive female rats independently of changes in blood pressure in a pre-clinical model of menopause.","authors":"W C Dos Santos, S N Ronchi, L A Gonçalves, L C S L Oliveira, G J Sousa, A F Melo Junior, T U de Andrade, N S Bissoli, G A Brasil","doi":"10.1590/1414-431X2025e14209","DOIUrl":"https://doi.org/10.1590/1414-431X2025e14209","url":null,"abstract":"Liraglutide (LIRA) is an agonist of the GLP-1 receptor used in the treatment of type 2 diabetes with a cardioprotective effect, although little is known about the effects of LIRA in post-menopause. We aimed to evaluate the effects of LIRA in the cardiovascular system of ovariectomized spontaneously hypertensive rats (SHR). SHR rats were separated into two groups: ovariectomized (saline) and ovariectomized + liraglutide (0.6 and 1.2 mg/kg for 4+4 weeks, respectively). Systolic blood pressure (SBP) was indirectly evaluated at the beginning and end of treatment. Diastolic, systolic, and mean blood pressure were evaluated in the carotid artery of anesthetized animals, while left ventricle systolic blood pressure (LVSBP) and left ventricle derivatives (-dP/dt; +dP/dt) were evaluated in the left ventricle. An oral glucose tolerance test (GTT) was conducted. Antioxidant enzymes and calcium-handling proteins were analyzed in heart tissue by western blot. Treatment with LIRA increased the expression of antioxidant enzymes (superoxide dismutase (SOD2) and catalase). No changes were observed in the GTT, cardiac hemodynamics, blood pressure, and calcium-handling protein expression. A decrease in visceral fat depot was observed without changes in final body weight. LIRA induced an antioxidant subclinical effect in ovariectomized SHR female rats without changing glucose metabolism and cardiac blood pressure.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e14209"},"PeriodicalIF":1.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11996164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynactin knockdown leads to synuclein aggregation by blocking autophagy in a zebrafish model of Parkinson's disease. 在斑马鱼帕金森病模型中，Dynactin敲低通过阻断自噬导致突触核蛋白聚集。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-03-24 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2025e14282

Yongmei Wu, Qiang Guo, Jinfan Gan, Linghan Duan, Haixia Zhao, Haoran Tai, Chan Yang, Yunzhu Li, Zhen Xu, Yue Yao, Zheng Nie, Ming Yang, Shurong Li, Jun Li, Bingyin Su

{"title":"Dynactin knockdown leads to synuclein aggregation by blocking autophagy in a zebrafish model of Parkinson's disease.","authors":"Yongmei Wu, Qiang Guo, Jinfan Gan, Linghan Duan, Haixia Zhao, Haoran Tai, Chan Yang, Yunzhu Li, Zhen Xu, Yue Yao, Zheng Nie, Ming Yang, Shurong Li, Jun Li, Bingyin Su","doi":"10.1590/1414-431X2025e14282","DOIUrl":"10.1590/1414-431X2025e14282","url":null,"abstract":"Axons of dopaminergic neurons projecting from substantia nigra to striatum are severely affected in the early stage of Parkinson's disease (PD), with axonal degeneration preceding the loss of cell bodies. Our previous study indicated that the dysfunctional retrograde axonal transport could lead to the death of dopaminergic neurons resulting in PD (10.1111/j.1471-4159.2008.05526.x). However, dynein, as the main molecule involved in retrograde axonal transport, was not affected. This study aimed to verify the hypothesis that dynactin rather than dynein may be one of the key factors in the retrograde degeneration of dopaminergic neurons in the early stage of PD. Dynactin morpholino was used to inhibit the expression of dynactin in transgenic (Vmat2:GFP) zebrafish, resulting in a significant decrease of diencephalon dopamine neurons and synuclein aggregation in the basal plate region. In the dopaminergic SH-SY5Y cell line, dynactin-siRNA knockdown resulted in the expression of dynein shifting from dispersed distribution to concentration in synapses and cytoplasm near axons, and the fusion rate of dynein to dynactin was decreased, especially in axons, which blocked the retrograde axonal transport of α-synuclein and autophagy flow. Our results linked the knockdown of dynactin gene to the dysfunction of axonal microtubule transport system, suggesting that dynactin may be one of the key factors contributing to the retrograde degeneration of dopaminergic neurons in the early stage of PD.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e14282"},"PeriodicalIF":1.9,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-intensity pulsed ultrasound inhibits chondrocyte senescence by inhibiting PI3K/AKT/mTOR signaling. 低强度脉冲超声通过抑制PI3K/AKT/mTOR信号抑制软骨细胞衰老。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-03-24 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2025e14408

Wang Han, Mengtong Guan, Bo Liao, Xiaoyu Han, Kaiting Li, Qing Chen, Xiya Guo, Yajuan Niu, Ying Zhu, Dingqun Bai

{"title":"Low-intensity pulsed ultrasound inhibits chondrocyte senescence by inhibiting PI3K/AKT/mTOR signaling.","authors":"Wang Han, Mengtong Guan, Bo Liao, Xiaoyu Han, Kaiting Li, Qing Chen, Xiya Guo, Yajuan Niu, Ying Zhu, Dingqun Bai","doi":"10.1590/1414-431X2025e14408","DOIUrl":"10.1590/1414-431X2025e14408","url":null,"abstract":"Cellular senescence is an important cause of age-related degenerative diseases, including osteoarthritis (OA). Chondrocyte senescence is crucial in OA onset and progression. As a non-invasive, safe, and widely used physical rehabilitation factor, the effect and mechanism of low intensity pulsed ultrasound (LIPUS) on chondrocyte senescence remain unclear. This study evaluated the inhibitory effect of LIPUS on OA chondrocyte senescence in vitro and in vivo. The effect of LIPUS on chondrocyte senescence was examined by RT-qPCR, enzyme-linked immunosorbent assay (ELISA), and western blotting. Changes in levels of reactive oxygen species (ROS) and γ-h2ax foci in senescent chondrocytes were detected using fluorescent staining. Chondrocyte senescence was evaluated by senescence-associated β-galactosidase (SA-β-gal) staining. The PI3K inhibitor LY294002 and the PI3K agonist 740Y-P were used to investigate whether PI3K/AKT/mTOR signalling was involved in the effect of LIPUS in senescent chondrocytes. Chondrocyte senescence and cartilage degeneration were analyzed in a destabilization of the medial meniscal (DMM) mouse model by immunohistochemistry, hematoxylin and eosin staining, and safranin-O/fast green staining. LIPUS inhibited the expression of the senescence-associated secretory phenotype (SASP) factors CCL4 and CCL2 and the senescence phenotype in doxorubicin-treated chondrocytes by inhibiting the PI3K/AKT/mTOR pathway. LIPUS alleviated chondrocyte senescence and attenuated OA progression in the DMM mice. These results demonstrated a novel role for LIPUS in inhibiting chondrocyte senescence and the SASP by modulating PI3K/AKT/mTOR signalling. Our findings expanded the clinical application of LIPUS and provide a new, non-invasive, and safe treatment approach to prevent and treat age-related degenerative joint disorders.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e14408"},"PeriodicalIF":1.9,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The extent of carbapenem-resistant encoding genes in Klebsiella pneumoniae from COVID-19 and non-COVID-19 patients in a tertiary care center, Saudi Arabia. 沙特阿拉伯三级保健中心来自COVID-19和非COVID-19患者的肺炎克雷伯菌中碳青霉烯耐药编码基因的程度

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-03-24 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2025e14066

A Al Bshabshe, M E Hamid, E Salem, I M Abdelrahim, M Assiry, A Alasmari, M Joseph, Y Alhammdi

{"title":"The extent of carbapenem-resistant encoding genes in Klebsiella pneumoniae from COVID-19 and non-COVID-19 patients in a tertiary care center, Saudi Arabia.","authors":"A Al Bshabshe, M E Hamid, E Salem, I M Abdelrahim, M Assiry, A Alasmari, M Joseph, Y Alhammdi","doi":"10.1590/1414-431X2025e14066","DOIUrl":"10.1590/1414-431X2025e14066","url":null,"abstract":"Rapid dissemination of Klebsiella pneumoniae carbapenemase (KPC) is a leading cause of treatment failure, significantly increasing morbidity and mortality rates among inpatients, particularly in the intensive care unit (ICU). This study aimed to detect the occurrence of carbapenemase- and carbapenem-resistant-encoding genes in K. pneumoniae isolates from COVID-19 positive and negative patients, and to assess their impact on patient outcomes. A prospective analysis was conducted at a tertiary care hospital in Saudi Arabia, collecting 97 carbapenem-resistant K. pneumoniae (CRKP) isolates from patients with COVID-19 during 2020-2021. Isolates were obtained from various clinical specimens. Antimicrobial susceptibility assays were performed using the Automated Vitek-2 system, and data were analyzed using IBM SPSS Statistics. The predominant carbapenemases identified were Oxacillinase-48 (OXA-48), followed by KPC and New Delhi metallo-β-lactamase (NDM), with Imipenemase (IMP) and Verona integron-encoded metallo-β-lactamase (VIM) being the least prevalent. COVID-19 did not significantly affect the distribution of these genes (P>0.05); however, COVID-19 status and age over 60 years significantly impacted the outcomes of CRKP patients. Other factors such as gender, total ICU or ward stay, and comorbidities did not significantly affect CRKP infection outcomes. The most common carbapenem-resistant genes identified were blaKPC, blaNDM, and blaOXA-48; however, they were not significantly associated with increased mortality.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e14066"},"PeriodicalIF":1.9,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of invasive yeast infections in a COVID-19 intensive care unit in northeastern Brazil. 巴西东北部COVID-19重症监护病房侵袭性酵母菌感染流行情况

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-03-24 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2025e13915

S M F R S Medeiros, S D Costa-Júnior, V P Perez, E S S Sousa, E H Campana, M A O Araújo, F Q S Guerra, N N Dejani, F O Souto

{"title":"Prevalence of invasive yeast infections in a COVID-19 intensive care unit in northeastern Brazil.","authors":"S M F R S Medeiros, S D Costa-Júnior, V P Perez, E S S Sousa, E H Campana, M A O Araújo, F Q S Guerra, N N Dejani, F O Souto","doi":"10.1590/1414-431X2025e13915","DOIUrl":"10.1590/1414-431X2025e13915","url":null,"abstract":"The COVID-19 pandemic has caused a global crisis, overwhelming hospitals and intensive care units (ICU) and leading to an increase in nosocomial infections due to prolonged hospitalization and other risk factors. The present study evaluated the prevalence of secondary fungal infections in critically ill patients with COVID-19. This is a retrospective, single-center study conducted in a hospital in northeastern Brazil, which evaluated 1,364 medical records of patients admitted to a COVID-19 ICU during 2020 and 2021. A total of 327 pathogenic yeasts were isolated from 132 (40.4%) respiratory, 70 (21.4%) blood, 124 (37.9%) urine, and one (0.3%) surgical wound samples. Fungal infections were diagnosed in the intermediate (5 to 12 days) or late (≥12 days) stage of hospitalization. The most frequent yeast isolated from critically ill COVID-19 patients was Candida albicans [126 (67.7%) and 60 (42.6%)], followed by Candida tropicalis [25 (13.4%) and 39 (27.7%)]. Candida parapsilosis isolates increased 5.7-fold in 2021 [40 (28.4%)] compared to 2020 [7 (3.8%)]. The least frequently isolated in 2020 and 2021 were Nakaseomyces glabratus [4 (2.2%) and 1 (0.7%)], and Pichia kudriavzevii, which was isolated only in 2021 (1 (0.7%)). During the study period, a decrease in susceptibility to antifungals was observed: susceptibility to voriconazole reduced from 100 to 77.2%, to flucytosine from 99.4 to 78.8%, and to micafungin from 99.4 to 83.6%. The changes in the frequency of species causing secondary infections in critically ill COVID-19 patients and susceptibility to the antifungals indicate the need for early and adequate diagnosis to minimize negative outcomes.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e13915"},"PeriodicalIF":1.9,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential of N-acetylcysteine in the management of low back pain: a scoping review of studies in humans and animal models. n -乙酰半胱氨酸在腰痛治疗中的潜力：对人类和动物模型研究的范围综述

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-03-24 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2025e14382

G Sinigaglia, L M Fortunato, M L Grillo, W A Partata

{"title":"Potential of N-acetylcysteine in the management of low back pain: a scoping review of studies in humans and animal models.","authors":"G Sinigaglia, L M Fortunato, M L Grillo, W A Partata","doi":"10.1590/1414-431X2025e14382","DOIUrl":"10.1590/1414-431X2025e14382","url":null,"abstract":"Low back pain (LBP) is a common type of pain that causes disability and impairs cognitive function. With over 80% of adults estimated to experience LBP during their lifetime, this type of pain not only has a significant impact on the individual, but also on public health systems and national economies. Unfortunately, there is no single standard of care for patients with LBP. N-acetylcysteine (NAC), which is used clinically to treat acetaminophen overdose, has recently been tested as a potential treatment for LBP. NAC is inexpensive and commercially available, and it has an established tolerance and safety profile. However, NAC's efficacy in LBP has not been established. This scoping review presents a summary of studies investigating the effects of NAC and the potential benefits in LBP treatment, and highlights its potential molecular mechanisms and side effects. A systematic literature search in Pubmed/MEDLINE, Embase, Scopus, Science Direct, Web of Science, Cinahl, and Lilacs databases was conducted. The PRISMA-ScR checklist was used to ensure integrity of the review. The scoping review protocol was registered in the Open Science Framework. No limit was set on study language and publication date. In total, 2357 articles were located, of which 16 were included. The studies show that NAC has potential for LBP treatment, but data are derived only from a few clinical trials and preclinical studies. Thus, there is much to learn and more clinical studies should be performed before NAC can be clinically recommended for the treatment of LBP.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e14382"},"PeriodicalIF":1.9,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Saccharomyces boulardii supplementation does not affect anaerobic power gain induced by short-term sprint interval training in physically active individuals. 补充博氏酵母菌不会影响体力活跃个体短期冲刺间歇训练引起的无氧能力增加。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-03-24 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2025e14196

A S R Hudson, A M T Lisboa, P V R Andrade, R S Bruzzi, Y A T Martins, D D Soares, F S Martins, S P Wanner

{"title":"Saccharomyces boulardii supplementation does not affect anaerobic power gain induced by short-term sprint interval training in physically active individuals.","authors":"A S R Hudson, A M T Lisboa, P V R Andrade, R S Bruzzi, Y A T Martins, D D Soares, F S Martins, S P Wanner","doi":"10.1590/1414-431X2025e14196","DOIUrl":"10.1590/1414-431X2025e14196","url":null,"abstract":"Sprint interval training (SIT), which consists of vigorous-intensity exercise interspersed with periods of rest or low-intensity exercise, can improve human anaerobic performance. Probiotic strains, including yeasts (e.g. Saccharomyces boulardii; Sb), have beneficial effects on human health; however, evidence regarding the effects of probiotics on anaerobic performance is unavailable. The current study investigated whether Sb supplementation influences the SIT-induced changes to the following performance variables: peak (PPO) and mean (MPO) power output. Fifteen healthy individuals (twelve men and three women) were randomly divided into two groups: placebo (PLA; n=8) and Sb (n=7). The individuals performed six SIT sessions on a cycle ergometer (four to seven 30-s all-out sprints thrice weekly). During the training period, participants ingested a capsule containing PLA or at least 1×109 Sb cells daily for 14 days. Performance-related variables were compared between the first and last training sessions. Sb supplementation did not influence the changes in PPO and MPO across the two weeks of training (P>0.05); therefore, the data from both groups were analyzed collectively to assess performance changes induced by SIT. Training increased PPO, an index of anaerobic power, in the sixth session compared to the first session (by 8±11% in the first sprint; +1.0±1.2 W/kg; P=0.008) but did not change MPO. In conclusion, short-term SIT improved the participants' anaerobic performance (power), as evidenced by increased PPO. Sb supplementation did not affect the improved anaerobic power caused by SIT.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e14196"},"PeriodicalIF":1.9,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isokinetic assessment of muscle function according to physical activity level and cardiovascular risk in asymptomatic adults aged 20 to 80 years. 20 - 80岁无症状成人身体活动水平和心血管风险对肌肉功能的等速评估

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-03-03 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2025e14214

V Z Dourado, M B Nascimento, R C Navarro, R P da Silva, B B Gonze, K M Guedes, V T Lauria, W O Vieira, T L V D P Ostolin

{"title":"Isokinetic assessment of muscle function according to physical activity level and cardiovascular risk in asymptomatic adults aged 20 to 80 years.","authors":"V Z Dourado, M B Nascimento, R C Navarro, R P da Silva, B B Gonze, K M Guedes, V T Lauria, W O Vieira, T L V D P Ostolin","doi":"10.1590/1414-431X2025e14214","DOIUrl":"10.1590/1414-431X2025e14214","url":null,"abstract":"As limb muscle function is age- and sex-related, both elbow and knee isokinetic muscle functions and their main predictors, such as physical activity level and cardiovascular risk factors, should be determined. We aimed to describe the percentiles of normality of the isokinetic muscle function of the knee and elbow joints. Secondarily, we developed equations to predict muscle function in apparently healthy adults aged 20-80 years, including cardiovascular risk factors. We conducted a cross-sectional study with 1,334 adults. We collected sociodemographic data, self-reported cardiovascular risk, anthropometry, body composition (bioelectrical impedance), moderate-to-vigorous physical activity (MVPA) (triaxial accelerometry), and isokinetic muscle function. Multiple regression analysis was used to develop equations to predict isokinetic muscle function. Percentiles of normality for muscle function were described by sex and age (20-39, 40-59, and >60 years). The models accounted for 49.6-70.9% of the total variability of muscle function, but MVPA and cardiovascular risk slightly influenced the coefficient of determination (additional ΔR2=0.003-0.006). Demographic and anthropometric variables were more relevant predictors of isokinetic muscle function (R2=0.50-0.70) than MVPA and cardiovascular risk. Even though they correlated with muscle function, cardiovascular risk and MVPA failed to explain the variability of muscle function largely determined by anthropometric and sociodemographic data. The percentile values and equations developed will help in interpreting the isokinetic muscle function and improve its clinical use.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e14214"},"PeriodicalIF":1.9,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rac1 overexpression promotes Treg-derived cytokines to mediate choroidal neovascularization in wet age-related macular degeneration. Rac1过表达促进treg来源的细胞因子介导湿性年龄相关性黄斑变性脉络膜新生血管。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-03-03 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2024e14187

Juanjuan Li, Yuling Ren, Hua Li, Zhikun Zheng

{"title":"Rac1 overexpression promotes Treg-derived cytokines to mediate choroidal neovascularization in wet age-related macular degeneration.","authors":"Juanjuan Li, Yuling Ren, Hua Li, Zhikun Zheng","doi":"10.1590/1414-431X2024e14187","DOIUrl":"10.1590/1414-431X2024e14187","url":null,"abstract":"Age-related macular degeneration (AMD), particularly the wet form characterized by choroidal neovascularization, is a leading cause of vision loss. Dysregulation of regulatory T cells (Tregs), key modulators of inflammatory responses, may contribute to wet AMD pathogenesis. This study explored the involvement of Tregs and the Rac1 signaling pathway in modulating Treg-derived cytokine expression and their role in choroidal neovascularization during wet AMD progression. Peripheral blood samples from healthy controls, dry AMD patients, and wet AMD patients were collected. An in vitro transmembrane co-culture system of Tregs and human choroidal endothelial cells (HCECs) was employed to investigate the impact of Tregs (with or without Rac1 silencing) on the angiogenic phenotype of HCECs. A mouse model of AMD was established to evaluate the effects of a Rac1 inhibitor and IL-10/TGF-β neutralization on Tregs and choroidal neovascularization. An increased Treg percentage in the CD4+ T lymphocyte population was found in the peripheral blood samples of wet AMD patients. Tregs from wet AMD patients showed an increased expression of Rac1 and an elevated production of IL-10 and TGF-β1. Rac1 silencing suppressed Treg stability and differentiation, and impaired the pro-angiogenic effect of Tregs on HCECs. In the animal model of AMD, the administration of a Rac1 inhibitor or neutralizing antibodies against IL-10/TGF-β1 reduced Treg abundance and attenuated choroidal neovascularization. Rac1 upregulation in Tregs promoted IL-10 and TGF-β1 production to mediate choroidal neovascularization in wet AMD. Targeting Rac1 and Treg-derived IL-10/TGF-β1 production in Tregs may serve as a strategy to ameliorate AMD progression.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e14187"},"PeriodicalIF":1.9,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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