Brazilian Journal of Medical and Biological Research最新文献_第10页

Poricoic acid A attenuates renal fibrosis by inhibiting endoplasmic reticulum stress-mediated apoptosis. 茯苓酸 A 可通过抑制内质网应激介导的细胞凋亡减轻肾脏纤维化。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-11-25 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e14249

Hui Zhao, Tian Liu, Chang-E Yang, Yue-Huan Hu, Yan Niu, Sheng-Ping Lei, Lin Chen, Ming-Xia Zhang

{"title":"Poricoic acid A attenuates renal fibrosis by inhibiting endoplasmic reticulum stress-mediated apoptosis.","authors":"Hui Zhao, Tian Liu, Chang-E Yang, Yue-Huan Hu, Yan Niu, Sheng-Ping Lei, Lin Chen, Ming-Xia Zhang","doi":"10.1590/1414-431X2024e14249","DOIUrl":"10.1590/1414-431X2024e14249","url":null,"abstract":"Renal fibrosis is a common manifestation in the progression of chronic kidney disease (CKD) to kidney failure. Currently, there is no available therapy to prevent the progression of renal fibrosis. Poricoic acid A (PAA) isolated from Poria cocos shows notable antifibrotic effects. However, its potential mechanism is still unclear. This study aimed to evaluate the effects and the potential mechanisms of PAA against renal fibrosis. A mouse model of renal fibrosis was established using unilateral ureteral obstruction (UUO). We showed that PAA administration significantly alleviated renal lesions and collagen deposition in UUO mice. Mice with UUO resulted in epithelial-to-mesenchymal transition (EMT) and the activation of endoplasmic reticulum stress (ERS) in the renal tissues, while PAA treatment significantly inhibited EMT and ERS activation. Additionally, PAA markedly alleviated ERS-mediated apoptosis in UUO mice. Molecular docking results indicated that PAA stably combined to GRP78 and ATF4. In conclusion, these results demonstrated that PAA possesses a significant bioactivity against renal fibrosis and its treatment mechanism might be the inhibition of ERS-mediated apoptosis.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"57 ","pages":"e14249"},"PeriodicalIF":1.9,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between sleep duration and prevalence of hypertension: the China Health and Nutrition Survey. 睡眠时间与高血压患病率的相关性：中国健康与营养调查。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-11-25 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13868

Hong-Shan Guan, Hai-Juan Shangguan

{"title":"Correlation between sleep duration and prevalence of hypertension: the China Health and Nutrition Survey.","authors":"Hong-Shan Guan, Hai-Juan Shangguan","doi":"10.1590/1414-431X2024e13868","DOIUrl":"10.1590/1414-431X2024e13868","url":null,"abstract":"It is increasingly thought that sleep is a lifestyle factor that contributes to hypertension. However, the correlation between sleep duration and hypertension in the Chinese population remains largely unexplored. This study utilized data from the 2009 China Health and Nutrition Survey to investigate the correlation between sleep duration and hypertension. Average hours of sleep per day were grouped into following categories: ≤6, 7-9, and ≥10 h. The frequency of hypertension and odds ratio (OR) were computed across different sleep duration categories. Individuals sleeping 7-9 h per day were designated as the control group. Logistic regression was utilized for multivariate analysis. Among the 9435 participants, the mean sleep duration was 7.9±1.2 h. The prevalence of hypertension was 34.1, 21.7, and 29.3% for individuals sleeping ≤6, 7-9, and ≥10 h per day, respectively. Following adjustments for age, gender, body mass index, and diabetes, a significant association was observed between prolonged (≥10 h) sleep duration and hypertension. Compared to those sleeping 7-9 h per day, the OR for hypertension was 1.21 (95%CI: 1.02-1.43, P=0.03) for individuals sleeping ≥10 h per day. This study suggested that sleeping ≥10 h per day is associated with a higher risk of hypertension in adults.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"57 ","pages":"e13868"},"PeriodicalIF":1.9,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physical exercise-mediated neuroprotective mechanisms in Parkinson's disease, Alzheimer's disease, and epilepsy. 体育锻炼介导的帕金森病、阿尔茨海默病和癫痫的神经保护机制。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-11-25 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e14094

R A Pinho, A P Muller, L F Marqueze, Z Radak, R M Arida

{"title":"Physical exercise-mediated neuroprotective mechanisms in Parkinson's disease, Alzheimer's disease, and epilepsy.","authors":"R A Pinho, A P Muller, L F Marqueze, Z Radak, R M Arida","doi":"10.1590/1414-431X2024e14094","DOIUrl":"10.1590/1414-431X2024e14094","url":null,"abstract":"Research suggests that physical exercise is associated with prevention and management of chronic diseases. The influence of physical exercise on brain function and metabolism and the mechanisms involved are well documented in the literature. This review provides a comprehensive overview of the potential implications of physical exercise and the molecular benefits of exercise in Parkinson's disease, Alzheimer's disease, and epilepsy. Here, we present an overview of the effects of exercise on various aspects of metabolism and brain function. To this end, we conducted an extensive literature search of the PubMed, Web of Science, and Google Scholar databases to identify articles published in the past two decades. This review delves into key aspects including the modulation of neuroinflammation, neurotrophic factors, and synaptic plasticity. Moreover, we explored the potential role of exercise in advancing therapeutic strategies for these chronic diseases. In conclusion, the review highlights the importance of regular physical exercise as a complementary non-pharmacological treatment for individuals with neurological disorders such as Alzheimer's, Parkinson's disease, and epilepsy.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"57 ","pages":"e14094"},"PeriodicalIF":1.9,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exercise: a non-drug strategy of NK cell activation. 运动：激活 NK 细胞的非药物策略。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-11-25 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e14144

Huixin Pan, Rui Meng, Zixuan Jia, Jing Zhang, Wen Ma, Youhan Liu, Qinglu Wang, Qiaoqiao Li

{"title":"Exercise: a non-drug strategy of NK cell activation.","authors":"Huixin Pan, Rui Meng, Zixuan Jia, Jing Zhang, Wen Ma, Youhan Liu, Qinglu Wang, Qiaoqiao Li","doi":"10.1590/1414-431X2024e14144","DOIUrl":"10.1590/1414-431X2024e14144","url":null,"abstract":"Natural killer (NK) cells are a critical component of the innate immune system and one of the immune cells most sensitive to exercise. So far, it is widely believed that moderate exercise can significantly enhance the proliferation and activity of NK cells, strengthening immune function. However, the impact of exercise on NK cells is a dynamic and complex process. In addition to the type of exercise, the frequency, intensity, and duration of exercise are also key factors. This article not only briefly summarizes the activation mechanisms of NK cells but also delves into the potential importance of exercise as a non-pharmacological strategy in modulating NK cell activity and enhancing the immune system. Emerging studies have indicated that the timing and regularity of exercise bouts might also influence NK cell responses. Moreover, the interaction between exercise and other components of the immune system, such as cytokines and chemokines, could further modulate the functionality of NK cells. The above research is of crucial significance for achieving a deeper understanding of the intricate connection between exercise and NK cell function, as well as the development of effective health promotion strategies. In addition, further research is needed to investigate the effects of long-term exercise on NK cell function and the interaction between exercise and NK cell-mediated immune responses. Translating these research findings into precisely tailored exercise programs for specific populations, taking into account factors like age, health status, and genetic predisposition, could potentially offer unprecedented prospects for further advancements in this burgeoning field of study.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"57 ","pages":"e14144"},"PeriodicalIF":1.9,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of Hibiscus sabdariffa and high-intensity interval training induces reduction in adiposity and beneficial metabolic adaptations in obesity without changes in lipid metabolism. 将木槿花与高强度间歇训练结合使用，可在不改变脂质代谢的情况下，减少肥胖症患者的脂肪含量，并诱导有益的代谢适应。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-11-04 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13676

D B O de Oliveira, M A Giordani, R A M Luvizotto, A F do Nascimento, M C Dos Santos, K C C Santos, A P Lima-Leopoldo, A S Leopoldo, M M Sugizaki

{"title":"Association of Hibiscus sabdariffa and high-intensity interval training induces reduction in adiposity and beneficial metabolic adaptations in obesity without changes in lipid metabolism.","authors":"D B O de Oliveira, M A Giordani, R A M Luvizotto, A F do Nascimento, M C Dos Santos, K C C Santos, A P Lima-Leopoldo, A S Leopoldo, M M Sugizaki","doi":"10.1590/1414-431X2024e13676","DOIUrl":"10.1590/1414-431X2024e13676","url":null,"abstract":"High-intensity interval training (HIIT) has stood out as a treatment for obesity, leading to adaptations of the cardiovascular system and reducing body adiposity. In addition, the search for alternative therapies for weight loss has intensified. The administration of Hibiscus sabdariffa (Hs) has been described as an efficient supplement in weight loss and in the treatment of metabolic changes associated with obesity. In this context, the objective was to investigate the effects of the association of Hs and HIIT on metabolic adaptations and lipid metabolism in obese rats. Wistars rats were subjected to obesity and subsequently randomized into 4 groups: obese (Ob), obese + HS (ObHs), obese + HIIT (ObHIIT), and obese + HS + HIIT (ObHsHIIT). For 8 weeks, ObHs and ObHsHIIT rats received Hs extract daily (150 mg/kg of body weight) and trained groups (ObHIIT and ObHsHIIT) were subjected to a HIIT program on a treadmill. Nutritional profile, glycemic curve, biochemical profile, and liver glycogen were determined. HIIT decreased caloric intake, feed efficiency, body adiposity, total body fat, and body weight gain, associated with improvements in physical performance parameters and a smaller glycemic curve and area. Hs had a hepatoprotective effect, reducing alkaline phosphatase values, but its effects were more pronounced when associated with HIIT. Therefore, the combination of treatments promoted a reduction in food consumption and body adiposity, as well as an improvement in physical performance and glycemic profile, but without changes in lipid metabolism.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"57 ","pages":"e13676"},"PeriodicalIF":1.9,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11540260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of COVID-19 cases treated in the intensive care unit in a coastal city hospital during the pandemic. 评估大流行期间在沿海城市医院重症监护室接受治疗的 COVID-19 病例。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-11-04 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e14301

P H A Klauss, E M B Hi, C C R Bianchi, A U Ruiz, M F C B de Barros, B M da Silva, T L Moretto, F G Soriano, R Curi, M C C Machado, R B Gritte

引用次数: 0

Antitumor activity of membranes associated with Acmella oleracea extract. 与黑醋栗提取物相关的膜的抗肿瘤活性。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-11-04 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e14129

C A Priante-Silva, B H Godoi, R F Menegon, N S da Silva, C Pacheco-Soares

{"title":"Antitumor activity of membranes associated with Acmella oleracea extract.","authors":"C A Priante-Silva, B H Godoi, R F Menegon, N S da Silva, C Pacheco-Soares","doi":"10.1590/1414-431X2024e14129","DOIUrl":"10.1590/1414-431X2024e14129","url":null,"abstract":"Epithelial cancers, such as epidermoid cancer and some adenocarcinomas, affect surface areas that are generally more accessible to various treatments. However, this group of tumor cells has an aggressive behavior, leading to a high annual mortality rate. The development of a biomaterial that is non-invasive, can kill tumor cells, and prevent opportunistic infections is the basis for the treatment for this type of cancer. Therefore, the objective of this study was to develop a biomaterial from chitosan and A. oleracea extracts that exhibits cytotoxic action against the HEp-2 tumor cell line. Dried crude 90% ethanol extracts were obtained through ultrasound-assisted maceration, followed by liquid-liquid extraction to yield the butanol fraction. From these extracts, chitosan membranes were developed and evaluated for their antitumor activity against HEp-2 using viability tests with crystal violet and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, in addition to a wound healing test. The cytotoxic assays indicated a significant reduction in cell density and mitochondrial activity, especially at the concentration of 1000 µg/mL of crude extract. The butanol fraction had minimal effects on mitochondrial activity. The wound healing test demonstrated that the biomaterial and extract prevented closure of the wound created in the cell monolayer within 48 h of incubation and caused changes in cell morphology. In view of this, we concluded that a chitosan membrane associated with a 90% ethanol extract of Acmella oleracea exhibited cytotoxic activity is a potential alternative treatment for superficial cancers.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"57 ","pages":"e14129"},"PeriodicalIF":1.9,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11540258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MRTO4 acts as an independent prognostic and immunological biomarker and is correlated with tumor microenvironment in hepatocellular carcinoma. MRTO4 是一种独立的预后和免疫生物标记物，与肝细胞癌的肿瘤微环境相关。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-11-04 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13780

Baobao Liang, Lan Li, Chenyang He, Meng Wang, Guochao Mao

{"title":"MRTO4 acts as an independent prognostic and immunological biomarker and is correlated with tumor microenvironment in hepatocellular carcinoma.","authors":"Baobao Liang, Lan Li, Chenyang He, Meng Wang, Guochao Mao","doi":"10.1590/1414-431X2024e13780","DOIUrl":"10.1590/1414-431X2024e13780","url":null,"abstract":"Liver cancer is a malignant tumor found worldwide. mRNA turnover 4 homolog (MRTO4) is highly expressed in hepatocellular carcinoma (HCC) tissues, and we explored its relationship with HCC. All cancer data were downloaded from the Cancer Genome Atlas (TCGA), the Cancer Immune Atlas (TCIA), and the Human Protein Atlas (THPA). Stromal scores, immune scores, and ESTIMATE scores were calculated by \"ESTIMATE\" R package. Single sample gene set enrichment analysis and CIBERSORT were used to evaluate the immune status and infiltration of cancer tissues. pRRophetic R package was used to predict the half-maximal inhibitory concentration (IC50) of different drugs in each sample. MRTO4 overexpression was associated with poor prognosis in HCC, and positively correlated with the stage and grade of HCC patients. The average immunophenoscore (IPS) of the low MRTO4 group was significantly higher than that of the high MRTO4 group. Tumor microenvironment (TME) scores were significantly higher in the low MRTO4 group than in the high MRTO4 group in HCC. MRTO4 expression was positively correlated with tumor mutation burden (TMB) and was positively correlated with most immune checkpoint gene expressions in HCC. Drug sensitivity analysis showed significantly higher IC50 values for 5-fluorouracil, gemcitabine, and sorafenib in patients with low MRTO4 expression than in those with high MRTO4 expression. MRTO4 acts as an independent prognostic and immunological biomarker and is correlated with clinical stage, tumor grade, and drug sensitivity in HCC. It may serve as a putative therapeutic target and potential biomarker for prognosis of HCC.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"57 ","pages":"e13780"},"PeriodicalIF":1.9,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11540254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurocognitive effects of proanthocyanidin in Alzheimer's disease: a systematic review of preclinical evidence. 原花青素对阿尔茨海默病的神经认知效应：临床前证据的系统回顾。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-11-04 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13587

A Reshma, A Subramanian, V Kumarasamy, T Tamilanban, M Sekar, S H Gan, V Subramaniyan, L S Wong, N N I M Rani, Y S Wu

{"title":"Neurocognitive effects of proanthocyanidin in Alzheimer's disease: a systematic review of preclinical evidence.","authors":"A Reshma, A Subramanian, V Kumarasamy, T Tamilanban, M Sekar, S H Gan, V Subramaniyan, L S Wong, N N I M Rani, Y S Wu","doi":"10.1590/1414-431X2024e13587","DOIUrl":"10.1590/1414-431X2024e13587","url":null,"abstract":"Cognitive disorders and dementia largely influence individual independence and orientation. Based on the Alzheimer's Disease International (ADI) estimation, approximately 75% of individuals with dementia are undiagnosed. In fact, in some low- and middle-income countries, the percentage is as high as 90%. In this systematic review, which is based on PRISMA guidelines, we aim to identify the mechanism of action of proanthocyanidin. Finding a natural product alternative as a potential nootropic can help increase the number of armamentariums against dementia and other cognitive impairments. In this preclinical research, we determined the effect of proanthocyanidins on Alzheimer's disease (AD) by searching electronic bibliographic databases like Scopus, Proquest, ScienceDirect, PubMed, and Google. There was no imposed time limit. However, the search was limited to only English articles. The review protocol is registered on PROSPERO as CRD42022356301. A population, intervention, control, and outcomes (PICO) technique was utilized for report inclusion, and all reports were assessed for risk of bias by using the SYRCLE's RoB tool. The article's bibliographic information, induction model, type of proanthocyanidins, animal strain/weight/age, and outcome measurements were acquired from ten papers and are reported here. Further analysis was validated and determined for the review. The included studies met the review's inclusion criteria and suggested that proanthocyanidins have a neurocognitive effect against AD. Additionally, the effectiveness of proanthocyanidins in reducing oxidative stress, acetylcholinesterase activity, amyloid beta, its efficacy in alleviating superoxide dismutase, cognitive properties, and in facilitating cholinergic transmission in various models of AD has been collectively observed in ten studies.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"57 ","pages":"e13587"},"PeriodicalIF":1.9,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11540257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Esculetin attenuates cerebral ischemia-reperfusion injury and protects neurons through Nrf2 activation in rats. 艾司奎林通过激活 Nrf2 减轻大鼠脑缺血再灌注损伤并保护神经元

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-11-04 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13914

Zhe Zhang, Jiayun Zhang, Rui Shi, Tiantian Xu, Shiduo Wang, Junbiao Tian

{"title":"Esculetin attenuates cerebral ischemia-reperfusion injury and protects neurons through Nrf2 activation in rats.","authors":"Zhe Zhang, Jiayun Zhang, Rui Shi, Tiantian Xu, Shiduo Wang, Junbiao Tian","doi":"10.1590/1414-431X2024e13914","DOIUrl":"10.1590/1414-431X2024e13914","url":null,"abstract":"Nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2) is a key transcription factor in the antioxidant response and is associated with various chronic diseases. The aim of this study was to explore the action of esculetin, a natural dihydroxy coumarin, on attenuating middle cerebral artery occlusion (MCAO) and reperfusion, and whether its effect is dependent on Nrf2 activation, as well as nuclear factor-kappa B (NF-κB) inhibition. Two doses of esculetin (20 and 40 mg/kg) were tested on rats with MCAO reperfusion. Neurological deficiency, oxidative stress, and pathological analyses were performed to evaluate its effect. An in vitro analysis was also used to confirm whether its action was dependent on the Nrf2/HO-1/NQO-1 pathway. Compared with MCAO reperfusion rats, esculetin improved infarct volume and increased normal-shaped neuron cells by decreasing tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, and IL-1β levels. The oxidative stress parameter malondialdehyde (MDA) decreased and the activity of superoxide dismutase (SOD) and glutathione/glutathione disulfide (GSH/GSSG) ratio increased after esculetin treatment. Moreover, esculetin inhibited NF-κB activation induced by MCAO. In vitro, hypoxia/reoxygenation (H/R) impaired the viability of rat neuron cells and esculetin showed a neuron protection effect on cells. Nrf2 inhibitor Brusatol inhibited the activation of Nrf2, heme oxygenase-1 (HO-1), and NAD(P)H quinone oxidoreductase 1 (NQO-1) caused by esculetin and abolished its protection effect. Esculetin protected cerebral neurons from ischemia-reperfusion injury by inhibiting NF-κB and Nrf2/HO-1/NQO-1 activation.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"57 ","pages":"e13914"},"PeriodicalIF":1.9,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11540255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0