Brazilian Journal of Medical and Biological Research最新文献

Association of Hibiscus sabdariffa and high-intensity interval training induces reduction in adiposity and beneficial metabolic adaptations in obesity without changes in lipid metabolism. 将木槿花与高强度间歇训练结合使用，可在不改变脂质代谢的情况下，减少肥胖症患者的脂肪含量，并诱导有益的代谢适应。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-11-04 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13676

D B O de Oliveira, M A Giordani, R A M Luvizotto, A F do Nascimento, M C Dos Santos, K C C Santos, A P Lima-Leopoldo, A S Leopoldo, M M Sugizaki

{"title":"Association of Hibiscus sabdariffa and high-intensity interval training induces reduction in adiposity and beneficial metabolic adaptations in obesity without changes in lipid metabolism.","authors":"D B O de Oliveira, M A Giordani, R A M Luvizotto, A F do Nascimento, M C Dos Santos, K C C Santos, A P Lima-Leopoldo, A S Leopoldo, M M Sugizaki","doi":"10.1590/1414-431X2024e13676","DOIUrl":"10.1590/1414-431X2024e13676","url":null,"abstract":"High-intensity interval training (HIIT) has stood out as a treatment for obesity, leading to adaptations of the cardiovascular system and reducing body adiposity. In addition, the search for alternative therapies for weight loss has intensified. The administration of Hibiscus sabdariffa (Hs) has been described as an efficient supplement in weight loss and in the treatment of metabolic changes associated with obesity. In this context, the objective was to investigate the effects of the association of Hs and HIIT on metabolic adaptations and lipid metabolism in obese rats. Wistars rats were subjected to obesity and subsequently randomized into 4 groups: obese (Ob), obese + HS (ObHs), obese + HIIT (ObHIIT), and obese + HS + HIIT (ObHsHIIT). For 8 weeks, ObHs and ObHsHIIT rats received Hs extract daily (150 mg/kg of body weight) and trained groups (ObHIIT and ObHsHIIT) were subjected to a HIIT program on a treadmill. Nutritional profile, glycemic curve, biochemical profile, and liver glycogen were determined. HIIT decreased caloric intake, feed efficiency, body adiposity, total body fat, and body weight gain, associated with improvements in physical performance parameters and a smaller glycemic curve and area. Hs had a hepatoprotective effect, reducing alkaline phosphatase values, but its effects were more pronounced when associated with HIIT. Therefore, the combination of treatments promoted a reduction in food consumption and body adiposity, as well as an improvement in physical performance and glycemic profile, but without changes in lipid metabolism.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11540260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of COVID-19 cases treated in the intensive care unit in a coastal city hospital during the pandemic. 评估大流行期间在沿海城市医院重症监护室接受治疗的 COVID-19 病例。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-11-04 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e14301

P H A Klauss, E M B Hi, C C R Bianchi, A U Ruiz, M F C B de Barros, B M da Silva, T L Moretto, F G Soriano, R Curi, M C C Machado, R B Gritte

引用次数: 0

Antitumor activity of membranes associated with Acmella oleracea extract. 与黑醋栗提取物相关的膜的抗肿瘤活性。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-11-04 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e14129

C A Priante-Silva, B H Godoi, R F Menegon, N S da Silva, C Pacheco-Soares

{"title":"Antitumor activity of membranes associated with Acmella oleracea extract.","authors":"C A Priante-Silva, B H Godoi, R F Menegon, N S da Silva, C Pacheco-Soares","doi":"10.1590/1414-431X2024e14129","DOIUrl":"10.1590/1414-431X2024e14129","url":null,"abstract":"Epithelial cancers, such as epidermoid cancer and some adenocarcinomas, affect surface areas that are generally more accessible to various treatments. However, this group of tumor cells has an aggressive behavior, leading to a high annual mortality rate. The development of a biomaterial that is non-invasive, can kill tumor cells, and prevent opportunistic infections is the basis for the treatment for this type of cancer. Therefore, the objective of this study was to develop a biomaterial from chitosan and A. oleracea extracts that exhibits cytotoxic action against the HEp-2 tumor cell line. Dried crude 90% ethanol extracts were obtained through ultrasound-assisted maceration, followed by liquid-liquid extraction to yield the butanol fraction. From these extracts, chitosan membranes were developed and evaluated for their antitumor activity against HEp-2 using viability tests with crystal violet and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, in addition to a wound healing test. The cytotoxic assays indicated a significant reduction in cell density and mitochondrial activity, especially at the concentration of 1000 µg/mL of crude extract. The butanol fraction had minimal effects on mitochondrial activity. The wound healing test demonstrated that the biomaterial and extract prevented closure of the wound created in the cell monolayer within 48 h of incubation and caused changes in cell morphology. In view of this, we concluded that a chitosan membrane associated with a 90% ethanol extract of Acmella oleracea exhibited cytotoxic activity is a potential alternative treatment for superficial cancers.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11540258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MRTO4 acts as an independent prognostic and immunological biomarker and is correlated with tumor microenvironment in hepatocellular carcinoma. MRTO4 是一种独立的预后和免疫生物标记物，与肝细胞癌的肿瘤微环境相关。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-11-04 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13780

Baobao Liang, Lan Li, Chenyang He, Meng Wang, Guochao Mao

{"title":"MRTO4 acts as an independent prognostic and immunological biomarker and is correlated with tumor microenvironment in hepatocellular carcinoma.","authors":"Baobao Liang, Lan Li, Chenyang He, Meng Wang, Guochao Mao","doi":"10.1590/1414-431X2024e13780","DOIUrl":"10.1590/1414-431X2024e13780","url":null,"abstract":"Liver cancer is a malignant tumor found worldwide. mRNA turnover 4 homolog (MRTO4) is highly expressed in hepatocellular carcinoma (HCC) tissues, and we explored its relationship with HCC. All cancer data were downloaded from the Cancer Genome Atlas (TCGA), the Cancer Immune Atlas (TCIA), and the Human Protein Atlas (THPA). Stromal scores, immune scores, and ESTIMATE scores were calculated by \"ESTIMATE\" R package. Single sample gene set enrichment analysis and CIBERSORT were used to evaluate the immune status and infiltration of cancer tissues. pRRophetic R package was used to predict the half-maximal inhibitory concentration (IC50) of different drugs in each sample. MRTO4 overexpression was associated with poor prognosis in HCC, and positively correlated with the stage and grade of HCC patients. The average immunophenoscore (IPS) of the low MRTO4 group was significantly higher than that of the high MRTO4 group. Tumor microenvironment (TME) scores were significantly higher in the low MRTO4 group than in the high MRTO4 group in HCC. MRTO4 expression was positively correlated with tumor mutation burden (TMB) and was positively correlated with most immune checkpoint gene expressions in HCC. Drug sensitivity analysis showed significantly higher IC50 values for 5-fluorouracil, gemcitabine, and sorafenib in patients with low MRTO4 expression than in those with high MRTO4 expression. MRTO4 acts as an independent prognostic and immunological biomarker and is correlated with clinical stage, tumor grade, and drug sensitivity in HCC. It may serve as a putative therapeutic target and potential biomarker for prognosis of HCC.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11540254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurocognitive effects of proanthocyanidin in Alzheimer's disease: a systematic review of preclinical evidence. 原花青素对阿尔茨海默病的神经认知效应：临床前证据的系统回顾。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-11-04 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13587

A Reshma, A Subramanian, V Kumarasamy, T Tamilanban, M Sekar, S H Gan, V Subramaniyan, L S Wong, N N I M Rani, Y S Wu

{"title":"Neurocognitive effects of proanthocyanidin in Alzheimer's disease: a systematic review of preclinical evidence.","authors":"A Reshma, A Subramanian, V Kumarasamy, T Tamilanban, M Sekar, S H Gan, V Subramaniyan, L S Wong, N N I M Rani, Y S Wu","doi":"10.1590/1414-431X2024e13587","DOIUrl":"10.1590/1414-431X2024e13587","url":null,"abstract":"Cognitive disorders and dementia largely influence individual independence and orientation. Based on the Alzheimer's Disease International (ADI) estimation, approximately 75% of individuals with dementia are undiagnosed. In fact, in some low- and middle-income countries, the percentage is as high as 90%. In this systematic review, which is based on PRISMA guidelines, we aim to identify the mechanism of action of proanthocyanidin. Finding a natural product alternative as a potential nootropic can help increase the number of armamentariums against dementia and other cognitive impairments. In this preclinical research, we determined the effect of proanthocyanidins on Alzheimer's disease (AD) by searching electronic bibliographic databases like Scopus, Proquest, ScienceDirect, PubMed, and Google. There was no imposed time limit. However, the search was limited to only English articles. The review protocol is registered on PROSPERO as CRD42022356301. A population, intervention, control, and outcomes (PICO) technique was utilized for report inclusion, and all reports were assessed for risk of bias by using the SYRCLE's RoB tool. The article's bibliographic information, induction model, type of proanthocyanidins, animal strain/weight/age, and outcome measurements were acquired from ten papers and are reported here. Further analysis was validated and determined for the review. The included studies met the review's inclusion criteria and suggested that proanthocyanidins have a neurocognitive effect against AD. Additionally, the effectiveness of proanthocyanidins in reducing oxidative stress, acetylcholinesterase activity, amyloid beta, its efficacy in alleviating superoxide dismutase, cognitive properties, and in facilitating cholinergic transmission in various models of AD has been collectively observed in ten studies.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11540257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic effects of whole-body vibration on postmenopausal women with osteoporosis: a systematic review and meta-analysis. 全身振动对绝经后骨质疏松症妇女的治疗效果：系统回顾和荟萃分析。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-11-04 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13996

Qian Li, Lichao Liang, Chengfei Gao, Beibei Zong

引用次数: 0

Esculetin attenuates cerebral ischemia-reperfusion injury and protects neurons through Nrf2 activation in rats. 艾司奎林通过激活 Nrf2 减轻大鼠脑缺血再灌注损伤并保护神经元

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-11-04 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13914

Zhe Zhang, Jiayun Zhang, Rui Shi, Tiantian Xu, Shiduo Wang, Junbiao Tian

{"title":"Esculetin attenuates cerebral ischemia-reperfusion injury and protects neurons through Nrf2 activation in rats.","authors":"Zhe Zhang, Jiayun Zhang, Rui Shi, Tiantian Xu, Shiduo Wang, Junbiao Tian","doi":"10.1590/1414-431X2024e13914","DOIUrl":"10.1590/1414-431X2024e13914","url":null,"abstract":"Nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2) is a key transcription factor in the antioxidant response and is associated with various chronic diseases. The aim of this study was to explore the action of esculetin, a natural dihydroxy coumarin, on attenuating middle cerebral artery occlusion (MCAO) and reperfusion, and whether its effect is dependent on Nrf2 activation, as well as nuclear factor-kappa B (NF-κB) inhibition. Two doses of esculetin (20 and 40 mg/kg) were tested on rats with MCAO reperfusion. Neurological deficiency, oxidative stress, and pathological analyses were performed to evaluate its effect. An in vitro analysis was also used to confirm whether its action was dependent on the Nrf2/HO-1/NQO-1 pathway. Compared with MCAO reperfusion rats, esculetin improved infarct volume and increased normal-shaped neuron cells by decreasing tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, and IL-1β levels. The oxidative stress parameter malondialdehyde (MDA) decreased and the activity of superoxide dismutase (SOD) and glutathione/glutathione disulfide (GSH/GSSG) ratio increased after esculetin treatment. Moreover, esculetin inhibited NF-κB activation induced by MCAO. In vitro, hypoxia/reoxygenation (H/R) impaired the viability of rat neuron cells and esculetin showed a neuron protection effect on cells. Nrf2 inhibitor Brusatol inhibited the activation of Nrf2, heme oxygenase-1 (HO-1), and NAD(P)H quinone oxidoreductase 1 (NQO-1) caused by esculetin and abolished its protection effect. Esculetin protected cerebral neurons from ischemia-reperfusion injury by inhibiting NF-κB and Nrf2/HO-1/NQO-1 activation.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11540255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Across-surface distances after one- and two-stage palatoplasty in children with oral cleft. 口腔裂隙患儿一期和二期腭成形术后的跨面距离。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-10-14 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13805

E C P Ambrosio, M T O P Bergamo, C Sforza, C F C Carrara, M A A M Machado, T M Oliveira

{"title":"Across-surface distances after one- and two-stage palatoplasty in children with oral cleft.","authors":"E C P Ambrosio, M T O P Bergamo, C Sforza, C F C Carrara, M A A M Machado, T M Oliveira","doi":"10.1590/1414-431X2024e13805","DOIUrl":"https://doi.org/10.1590/1414-431X2024e13805","url":null,"abstract":"The goal of this study was to digitally evaluate the development of maxillary dental arches of children with unilateral cleft lip and palate treated with one- and two-stage palatal closure. One hundred and sixty-eight digitized dental models of cheiloplasty and one-stage palatoplasty (G1) and cheiloplasty and two-stage palatoplasty (G2) were evaluated at preoperative time 1 (T1), preoperative time 2 (T2), and postoperative (T3). The following surface distances were evaluated: across surface distance; cleft widths anterior (P-P') and posterior (U-U') cleft widths, intercanine width (C-C'), and intertuberosity width (T-T'); smallest (P'-T') and largest (P-T) segment lengths; and smallest (C'-D') and largest (C-D) segment cleft depths. In G1, P-P', U-U', and C-C' reduced at T2, unlike P'-T' (P<0.05). P-T and C'-D' distances increased at T3 (P<0.05), while C-D increased at all stages (P<0.001). In G2, U-U' and C-C' reduced at T2 (P<0.05), while P'-T', P-T, C'-D', and C-D' increased at T3 (P<0.001). In an intergroup analysis of growth rate, G2 showed higher growth percentages compared to G1, in which C'-D' was significant (P=0.038). Furthermore, C'-D' presented a coefficient of determination of 0.076 (P=0.039). In conclusion, dental arch development is influenced by the rehabilitation protocol. However, in the sample evaluated, the comparison suggested that individuals whose palate was operated on in two stages had the most favorable palatal growth.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11484350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CAR-T therapy for gastrointestinal cancers: current status, challenges, and future directions. 胃肠道癌症的 CAR-T 疗法：现状、挑战和未来方向。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-10-14 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13640

Weidong Li, Yueming Huang, Xinhao Zhou, Bohao Cheng, Haitao Wang, Yao Wang

引用次数: 0

Analysis of the differences in immune-related genes and immune cell subtypes in acute myocardial infarction. 分析急性心肌梗死中免疫相关基因和免疫细胞亚型的差异。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-10-14 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e14345

Zhengmei Li, Ling Kang, Ke Jiang

{"title":"Analysis of the differences in immune-related genes and immune cell subtypes in acute myocardial infarction.","authors":"Zhengmei Li, Ling Kang, Ke Jiang","doi":"10.1590/1414-431X2024e14345","DOIUrl":"https://doi.org/10.1590/1414-431X2024e14345","url":null,"abstract":"Acute myocardial infarction (AMI) continues to be a leading cause of death globally, with distinct immune cell dynamics in ST-segment elevation myocardial infarction (STEMI) and non-ST-segment elevation myocardial infarction (NSTEMI) playing a critical role in disease progression and patient outcomes. Sample data for STEMI and NSTEMI were downloaded from the Sequence Read Archive (SRA) database (https://www.ncbi.nlm.nih.gov/sra). Differences and correlations of immune infiltrating cells were assessed by CIBERSORT. Differentially expressed genes (DEGs) were identified between STEMI and NSTEMI, followed by functional analysis. Immune-related DEGs were further identified. Some immune-related DEGs were selected to perform expression verification using real-time PCR. There was a significant difference in immune cells between STEMI and NSTEMI, including activated dendritic cells, memory CD4 T cells, mast cells, and CD8 T cells. A total of 229 DEGs were identified, with functions related to inflammatory regulation and drug metabolism. A total of 21 immune-related DEGs, which may play important roles in STEMI and NSTEMI, were identified. Among the 21 immune-related DEGs, genes like CCL18, NRP2, CXCR2, CXCL9, KIR2DL4, BPIFB1, and IL33 were significantly correlated with immune cells and had a tendency for differential expression between STEMI and NSTEMI patients. Our study reveals differences in the distribution of immune cell subsets between STEMI and NSTEMI, highlighting key immune-related genes and their association with immune cells, which may provide new insights into the treatment of AMI.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11484353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0