Brazilian Journal of Medical and Biological Research最新文献

Epidemiological and clinical factors of breast cancer in a population of mostly Afro-descendant women. 以非洲裔妇女为主的人群中乳腺癌的流行病学和临床因素。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-09-12 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2025e14749

J C B Neiva, M D B Fernandes, M B P Toralles, R S Andrade, K J Erfani, M E F Maia, J M Santana, I M M Silva, J B L Reis, S O T Klein

{"title":"Epidemiological and clinical factors of breast cancer in a population of mostly Afro-descendant women.","authors":"J C B Neiva, M D B Fernandes, M B P Toralles, R S Andrade, K J Erfani, M E F Maia, J M Santana, I M M Silva, J B L Reis, S O T Klein","doi":"10.1590/1414-431X2025e14749","DOIUrl":"10.1590/1414-431X2025e14749","url":null,"abstract":"Breast cancer is the most frequent neoplasm and has the highest mortality rate among women. In the Afro-descendant population, these tumors may appear earlier and assume a more aggressive behavior. This study aimed to assess the epidemiological and clinical behavior of breast cancer in a predominantly Afro-descendant population, identify risk and prognostic factors, and compare them with already available data. Clinical and sociodemographic data were obtained from medical records and interviews with the patients involved. The variables ethnicity, age, number of children, monthly income, and education were used to describe the epidemiological profile and the results of clinical evaluation and pathological anatomy study. The immunohistochemical analysis was used to correlate the clinical characteristics of the tumors and prognosis. Afro-descendant women represented 77% of the population and the mean age at diagnosis was 54.4 years. Approximately 75% had up to 2 children, 20.5% had low income, and 37.3% had a low level of education. Infiltrating ductal carcinoma was diagnosed in 91% of patients, 70.2% had a moderate degree of differentiation, luminal subtype A was the most prevalent (39%), and a higher than global average percentage had a triple negative profile (22.9%). Early stages were identified in 53.4% of patients and only 4.8% were diagnosed with metastatic disease. The recurrence rate was 11.6%, and the mortality rate was 6.8%. The present study showed that unfavorable sociodemographic and clinical aspects, such as the high prevalence of triple-negative tumors, were not associated with a worse prognosis.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e14749"},"PeriodicalIF":1.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12435623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between pre-stroke frailty status and post-stroke cognitive impairment in patients with acute large artery atherosclerotic cerebral infarction. 急性大动脉粥样硬化性脑梗死患者卒中前虚弱状态与卒中后认知障碍的关系

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-09-12 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2025e14837

Jun Wang, Yanrong Yuan, Yan Zhang, Huili Liu, Yongxing Yan

{"title":"Association between pre-stroke frailty status and post-stroke cognitive impairment in patients with acute large artery atherosclerotic cerebral infarction.","authors":"Jun Wang, Yanrong Yuan, Yan Zhang, Huili Liu, Yongxing Yan","doi":"10.1590/1414-431X2025e14837","DOIUrl":"10.1590/1414-431X2025e14837","url":null,"abstract":"The aim of this study was to investigate the correlation between pre-stroke frailty status and post-stroke cognitive impairment (PSCI) in patients with acute large artery atherosclerotic cerebral infarction. One hundred and eight patients with acute large artery atherosclerotic cerebral infarction admitted in our hospital from July 2020 to July 2023 were prospectively enrolled. Patients were stratified into frailty (46 cases) and non-frailty groups (62 cases) based on FRAIL scale scores. During the 6-month follow-up after the onset of cerebral infarction, patients were evaluated using the Chinese modified version of Montreal Cognitive Assessment (MoCA) scale for cognitive function and were divided into PSCI (52 cases) and non-PSCI (56 cases) groups. The frailty group showed significantly higher age, prevalence of hypertension and diabetes comorbidities, smoking and alcohol consumption rates, National Institutes of Health Stroke Scale (NHISS) score, and Modified Rankin Scale (mRS) score than those in the non-frailty group (P<0.05, P<0.01). The incidence of PSCI in the frailty group was also significantly higher than that in the non-frailty group (78.3 vs 25.8%, P<0.01). Compared to the non-PSCI group, the PSCI group had higher age, shorter education duration, fewer cases of reperfusion therapy, and greater frailty (P<0.05, P<0.01). Logistic regression analysis showed that pre-stroke frailty was an independent risk factor for PSCI (P<0.01). Timely assessment of the frailty status in patients with acute large artery atherosclerotic cerebral infarction is beneficial for preventing, delaying onset, and reducing the incidence of PSCI.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e14837"},"PeriodicalIF":1.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12435624/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LTF regulates glioblastoma progression and temozolomide resistance via the NF-κB signaling pathway. LTF通过NF-κB信号通路调节胶质母细胞瘤的进展和替莫唑胺耐药性。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-09-12 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2025e13821

Lili Gao, Hongbo Xiao, Hongyu Lu, Jun Ma, Haifeng Zhu

{"title":"LTF regulates glioblastoma progression and temozolomide resistance via the NF-κB signaling pathway.","authors":"Lili Gao, Hongbo Xiao, Hongyu Lu, Jun Ma, Haifeng Zhu","doi":"10.1590/1414-431X2025e13821","DOIUrl":"10.1590/1414-431X2025e13821","url":null,"abstract":"Glioblastoma (GBM) is the most prevalent tumor in the central nervous system in adults. Lactotransferrin (LTF) is a molecule involved in the growth of various tumors. However, the underlying mechanism of LTF in GBM progression and chemotherapy resistance remains unclear. In this study, the clinical and diagnostic value of LTF were evaluated. In vitro and in vivo experiments were performed to explore the functional role of LTF in GBM. Immunoprecipitation and immunofluorescence assays were performed to clarify the effect of LTF on nuclear factor-κB (NF-κB) activation. LTF was overexpressed in GBM and correlated with poor prognosis. LTF promoted GBM cell proliferation, invasion, and temozolomide (TMZ) resistance. Mechanism assay results indicated that LTF competitively binds to p65, rescuing the inhibited effect of PP2A on p65 phosphorylation, thereby activating the NF-κB signaling pathway. Our results confirmed that highly expressed LTF promoted GBM progression and TMZ resistance through the NF-κB signaling pathway.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e13821"},"PeriodicalIF":1.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12435622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Procedures for determining the antimicrobial activity of maggot excretions and secretions of the green bottle fly: a narrative literature review. 测定绿瓶蝇蛆排泄物和分泌物抑菌活性的方法：叙述性文献综述。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-09-12 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2025e14851

D L Dallavecchia, A A de Lima E Silva, A C M Debelian, V M Aguiar, R G Silva Filho

{"title":"Procedures for determining the antimicrobial activity of maggot excretions and secretions of the green bottle fly: a narrative literature review.","authors":"D L Dallavecchia, A A de Lima E Silva, A C M Debelian, V M Aguiar, R G Silva Filho","doi":"10.1590/1414-431X2025e14851","DOIUrl":"10.1590/1414-431X2025e14851","url":null,"abstract":"The therapeutic benefits obtained from the presence of larvae of certain flies in infected wounds have been mentioned since ancient times. Currently, the so-called maggot therapy or biosurgery is considered a simple, safe, relatively low-cost, and highly effective alternative for treating a wide variety of infected, necrotic, and difficult-to-heal wounds, including those caused by multidrug-resistant bacteria. In addition to the debridement of necrotic wound tissue promoted by larvae, especially from the green bottle fly (Lucilia sericata; Diptera: Calliphoridae), there is much evidence that their native excretions and secretions (NES) contain components with varied antimicrobial activity against Gram-positive and Gram-negative bacteria and activity against fungi. Furthermore, studies have shown the antibiofilm effect of NES. Biofilms represent an additional problem for wound healing because they prevent the action of antibiotics on the pathogens infecting the wound. The antimicrobial effects of crude NES or its molecular components described in studies sometimes present contrasting results when compared. This is probably due to the laboratory methodological aspects used, which range from the preparation of larvae and extraction of NES to the tests used to evaluate their antimicrobial activity. This review aimed to bring together a diversity of laboratory procedures and results that have been described for the antimicrobial potency of NES. Moreover, it aimed to contribute to a greater standardization of the methodologies adopted in new studies to generate more consensual and comparable results.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e14851"},"PeriodicalIF":1.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12435625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chrysin induces cell death and inhibits migration and invasion in squamous cervical carcinoma using a three-dimensional cell culture model. 用三维细胞培养模型研究菊花素诱导宫颈鳞癌细胞死亡并抑制细胞迁移和侵袭。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-08-29 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2025e14692

N L Mari, M V F de Souza, L E de F Meirelles, A R B de A Carvalho, C S Shinobu-Mesquita, M L Bruschi, M E L Consolaro, V R S da Silva

{"title":"Chrysin induces cell death and inhibits migration and invasion in squamous cervical carcinoma using a three-dimensional cell culture model.","authors":"N L Mari, M V F de Souza, L E de F Meirelles, A R B de A Carvalho, C S Shinobu-Mesquita, M L Bruschi, M E L Consolaro, V R S da Silva","doi":"10.1590/1414-431X2025e14692","DOIUrl":"10.1590/1414-431X2025e14692","url":null,"abstract":"Cervical cancer remains a leading cause of cancer-related mortality among women worldwide, despite treatment advances. The most common form is squamous cell cervical carcinoma, primarily associated with human papillomavirus (HPV) type 16. Chrysin (5,7-dihydroxyflavone) is a natural flavonoid with promising anticancer properties both in vitro and in vivo. The aim of this study was to evaluate the antiproliferative, anti-migratory, and anti-invasive effects of chrysin on the SiHa human cervical cancer cell line (HPV-16-positive) using a 3D cell culture model with spheroids. Cell viability was assessed using the resazurin assay, while cytostatic effects were monitored by measuring spheroid size through imaging. Migration was evaluated with the spheroid migration assay. The expression of matrix metalloproteinase (MMP)-2, MMP-9, and vascular endothelial growth factor (VEGF) was quantified by immunoenzymatic assays. Chrysin treatment exhibited concentration-dependent cytotoxic and cytostatic effects, reducing cell proliferation and decreasing SiHa spheroid size. Additionally, chrysin inhibited cell migration and invasion, potentially reducing metastatic potential, primarily by decreasing the production of MMP-2 and VEGF. These findings suggest that chrysin has therapeutic potential for squamous cell cervical carcinoma and warrants further in vivo preclinical studies.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e14692"},"PeriodicalIF":1.9,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of autonomic effects of ketamine-xylazine in normotensive and hypertensive rats. 氯胺酮-噻嗪对正常和高血压大鼠自主神经作用的比较分析。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-08-29 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2025e14598

P S Santos, J L Pereira Júnior, S S P Araújo, J A da Silva, A F M da Silva, J G V de Assunção, R G Silva, A A de Oliveira, F V S Nunes, M S Noleto, A P de Oliveira, R N Soriano, L G S Branco, H C Salgado, J P J Sabino

{"title":"Comparative analysis of autonomic effects of ketamine-xylazine in normotensive and hypertensive rats.","authors":"P S Santos, J L Pereira Júnior, S S P Araújo, J A da Silva, A F M da Silva, J G V de Assunção, R G Silva, A A de Oliveira, F V S Nunes, M S Noleto, A P de Oliveira, R N Soriano, L G S Branco, H C Salgado, J P J Sabino","doi":"10.1590/1414-431X2025e14598","DOIUrl":"10.1590/1414-431X2025e14598","url":null,"abstract":"This study aimed to evaluate the effects of the ketamine-xylazine (KX) anesthetic mixture on autonomic and cardiovascular functions in normotensive rats (Wistar) and spontaneously hypertensive rats (SHR), using both spectral and symbolic analyses. Male Wistar (n=22) and SHR (n=28) rats were intramuscularly anesthetized with KX, and their femoral artery and vein were cannulated for pulsatile arterial pressure recording and drug administration. Autonomic function was assessed 24 and 48 h post-surgery through spectral and symbolic analyses of heart rate (HR) and systolic arterial pressure (SAP) variability. KX anesthesia significantly decreased mean arterial pressure (MAP) 24 h post-surgery in both Wistar and SHR rats. Spectral analysis revealed increased sympathetic modulation in the vascular bed of SHR 48 h post-surgery. In Wistar rats, there was a significant reduction in parasympathetic modulation at 48 h, as indicated by root mean square of successive RR interval differences (RMSSD) and high frequency (HF) (nu) indices. Symbolic analysis, however, detected no significant changes in autonomic modulation. These data are consistent with the notion that KX anesthesia significantly impacts autonomic and cardiovascular functions, with differential effects observed between Wistar and SHR rats. Spectral analysis proved more effective than symbolic analysis in detecting these changes. These findings highlight the need for careful consideration of anesthetic effects in experimental research and suggest that optimizing anesthetic protocols could improve clinical outcomes by minimizing adverse autonomic impact.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e14598"},"PeriodicalIF":1.9,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of serum biomarkers associated with microvascular functions in a long-term high-fat diet-induced obesity rat model. 长期高脂饮食诱导的肥胖大鼠模型中微血管功能相关血清生物标志物的鉴定

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-08-29 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2025e14811

S Elmas, E G Cekic, P Cenik, F Sirinyildiz, O Elmas, O Elmas, G Cesur

{"title":"Identification of serum biomarkers associated with microvascular functions in a long-term high-fat diet-induced obesity rat model.","authors":"S Elmas, E G Cekic, P Cenik, F Sirinyildiz, O Elmas, O Elmas, G Cesur","doi":"10.1590/1414-431X2025e14811","DOIUrl":"10.1590/1414-431X2025e14811","url":null,"abstract":"Serum biomarkers are crucial for identifying complications of obesity. This study evaluated serum levels of leptin, vascular endothelial growth factor (VEGF), myeloperoxidase (MPO), C-reactive protein (CRP), oxidized low-density lipoprotein (OXLDL), low-density lipoprotein (LDL), and other biochemical parameters in a rat model of high-fat diet (HFD)-induced obesity to investigate potential relationships between these biomarkers and microvascular function. Rats in the HFD group were fed a high-fat diet for 23 weeks, whereas control rats received a standard diet. Microvascular function was assessed using the post-occlusive reactive hyperemia (PORH) test; PORH responses were measured in the right forelimbs using laser Doppler flowmetry. Serum samples were then collected to measure the aforementioned biomarkers. Results showed decreased PORH responses in the HFD group, indicating impaired microvascular function. Serum levels of leptin, MPO, CRP, LDL, and OXLDL were significantly higher in the HFD group. Strong correlations were observed between microvascular dysfunction and LDL, OXLDL, MPO, and CRP. No significant changes were found in VEGF or HDL levels. These findings suggest that increased LDL oxidation to OXLDL in obesity contributes to vascular impairment, likely due to increased oxidative stress and inflammation mediated by elevated MPO and CRP. Further research focusing on the roles of LDL, OXLDL, MPO, and CRP may provide deeper insights into the mechanisms underlying microvascular dysfunction in obesity.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e14811"},"PeriodicalIF":1.9,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of ayahuasca in preclinical studies with animals: a systematic review. 死藤水在动物临床前研究中的作用：系统综述。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-08-29 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2025e14687

A Walsh-Monteiro, S Morato, F A R Uribe, A Gouveia, J S Pedroso

{"title":"Effects of ayahuasca in preclinical studies with animals: a systematic review.","authors":"A Walsh-Monteiro, S Morato, F A R Uribe, A Gouveia, J S Pedroso","doi":"10.1590/1414-431X2025e14687","DOIUrl":"10.1590/1414-431X2025e14687","url":null,"abstract":"This systematic review investigates the effects of the ayahuasca beverage (Aya) in various animal models. Using the PRISMA protocol and adhering to the Cochrane Handbook for Systematic Reviews, a comprehensive selection of 2,359 documents was identified from the Web of Science, Medline, and Scopus databases between 2012 and 2022. Following the inclusion and exclusion criteria, 14 articles were included in the final analysis. The analysis revealed a diversity in the selection of animal models that included different developmental stages and various forms of Aya intoxication, including acute and chronic doses, and varying concentrations of the active principles. The studies revealed that Aya causes significant alterations in the motor and cognitive behavior of animals, especially associated with the serotonergic system, which seems to contribute to the negative symptoms also observed in schizophrenia and depression. Despite the evidence found, this review highlights the scarcity of more robust pre-clinical studies with methodological standardization to make more conclusive comparisons, especially given the need to identify potential toxic and neurochemical effects of Aya on organisms for a safer assessment of its therapeutic use.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e14687"},"PeriodicalIF":1.9,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dynamic changes in quantitative parameters of diffusion-weighted imaging at different b-values in a prostate cancer mouse model and their correlation with histopathology parameters. 前列腺癌小鼠模型不同b值弥散加权成像定量参数的动态变化及其与组织病理学参数的相关性

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-08-29 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2025e14527

Xin Zhou, Yida Li, Xinyuan Zhang, Mengmeng Zhang, Renfu Zhang, Shengjian Sun, Guohua Li

{"title":"The dynamic changes in quantitative parameters of diffusion-weighted imaging at different b-values in a prostate cancer mouse model and their correlation with histopathology parameters.","authors":"Xin Zhou, Yida Li, Xinyuan Zhang, Mengmeng Zhang, Renfu Zhang, Shengjian Sun, Guohua Li","doi":"10.1590/1414-431X2025e14527","DOIUrl":"10.1590/1414-431X2025e14527","url":null,"abstract":"The aim of this study was to evaluate the dynamic variations in the quantitative parameters of diffusion-weighted imaging (DWI) at different b-value combinations in a prostate cancer (PCa) mouse model for noninvasive monitoring of histopathological changes. Twenty-five male C57BL/6J mice were randomly allocated into a control group (n=5) or an experimental group (n=20). The experimental groups were used to establish the PCa model. On days 9, 12, 15, and 18 post-modeling, 5 mice were randomly selected for MRI, including T1WI, T2WI, T2WI SPIR, and DWI. The b-values were set at 0, 500, 1000, 1500, and 2000 s/mm2. Apparent diffusion coefficient (ADC) and exponential apparent diffusion coefficient (EADC) values from different b-value combinations were measured. Post-MRI, tumors were excised for histopathological analysis. DWI quantitative parameters, tumor nuclear fraction, and Ki-67 area fraction were compared on different days, along with correlation analysis. ADC values gradually decreased as tumor progressed, whereas EADC values gradually increased. Tumor nuclear fraction increased over time. Ki-67 increased first and then decreased. Tumor nuclear fraction was negatively correlated with the ADC value and positively correlated with the EADC value. The Ki-67 was positively correlated with the ADC value and negatively correlated with the EADC value. ADC values at b=1000, 1500 s/mm2 and the EADC values at b=0, 500 s/mm2 demonstrated the strongest correlations with the tumor nuclear fraction; the ADC and EADC values at b=500, 1000 s/mm2 were more strongly correlated with Ki-67, being potential noninvasive imaging biomarkers for monitoring changes in tumor histopathology.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e14527"},"PeriodicalIF":1.9,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strength training promotes attenuation of fatty liver while improving insulin resistance and inflammatory biomarkers in obese rats induced by high-fat diet. 力量训练促进脂肪肝的衰减，同时改善高脂肪饮食诱导的肥胖大鼠的胰岛素抵抗和炎症生物标志物。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2025-08-22 eCollection Date: 2025-01-01 DOI: 10.1590/1414-431X2025e14505

S Torezani-Sales, A P L Damiani, A R Madureira, J P Cordeiro, G H Taufner, B V Nogueira, M C Dias, M M Sugizaki, A S Leopoldo, A P Lima-Leopoldo

{"title":"Strength training promotes attenuation of fatty liver while improving insulin resistance and inflammatory biomarkers in obese rats induced by high-fat diet.","authors":"S Torezani-Sales, A P L Damiani, A R Madureira, J P Cordeiro, G H Taufner, B V Nogueira, M C Dias, M M Sugizaki, A S Leopoldo, A P Lima-Leopoldo","doi":"10.1590/1414-431X2025e14505","DOIUrl":"10.1590/1414-431X2025e14505","url":null,"abstract":"Obesity is an independent risk factor for metabolic dysfunction-associated steatotic liver disease (MASLD). Non-pharmacological strategies, such as strength training (ST), have been investigated for their effectiveness in attenuating MASLD. This study evaluated the effects of ST on hepatic fat accumulation in an experimental model of obesity. Thirty-day-old male Wistar rats (∼150 g) were assigned to either a standard diet (SD) or a high-fat diet (HFD). The experimental protocol lasted 26 weeks and was divided into two phases: 1) obesity induction and maintenance (16 weeks) and 2) ST intervention (10 weeks). After the 16th week, HFD-fed rats were further divided into sedentary obese (Ob) and obese trained (ObST) groups. The ST protocol consisted of 4-5 vertical ladder climbs with 60-s intervals, three times per week, using 50-100% of maximal load. Body weight (BW), fat pads, total body fat (BF), adiposity index (AI), and muscle strength were assessed, as were glycemic, lipid, inflammatory, and histological parameters. ST reduced BW, epididymal and visceral fat depots, triglycerides, total cholesterol, glucose, leptin, and tumor necrosis factor (TNF)-α levels while improving insulin resistance. In conclusion, ST significantly attenuated hepatic steatosis in obesity, promoting metabolic and anti-inflammatory benefits. These findings suggest that ST may be an effective therapeutic strategy for MASLD, and further studies are needed to elucidate its molecular mechanisms and clinical applications.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e14505"},"PeriodicalIF":1.9,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12377705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0