Brazilian Journal of Medical and Biological Research最新文献

{"title":"Set binding factor 1 (SBF1/MTMR5) as a potential novel target in colorectal cancer: an exploratory immunohistochemical and bioinformatics study.","authors":"A Alatawi, I Alhabib, M Fagere, F A Al-Jindan, K Alsamman, F M Alzahrani, A Y Owaidah, O S El-Masry","doi":"10.1590/1414-431X2026e15598","DOIUrl":"10.1590/1414-431X2026e15598","url":null,"abstract":"<p><p>Colorectal cancer (CRC) is a major cause of cancer-related morbidity and mortality worldwide, and dysregulation of phosphoinositides signaling pathways plays a critical role in its pathogenesis. The involvement of Set Binding Factor 1 (SBF1; MTMR5), a pseudophosphatase of the myotubularin family in CRC, remains unexplored. This study compiled experimental and bioinformatics approaches to investigate the expression and potential relevance of SBF1 in CRC. Immunohistochemical analysis was performed on CRC tissue specimens and corresponding normal adjacent tissues (NAT), with estimation of SBF1 staining followed by statistical comparison. In parallel, bioinformatics assessed gene ontology and protein-protein interactions, and open CRC datasets were analyzed to assess SBF1 alterations, expression profiles, and survival associations. The results demonstrated a significant overexpression of SBF1 in CRC tissues compared with NAT. Bioinformatics revealed SBF1 as a partner within a functional network enriched in phosphoinositide metabolism. Bioinformatics identified SBF1 genetic alterations in approximately 5% of cases, and survival analysis indicated that wild-type SBF1 could be an unfavorable prognostic marker, as reflected by hazard assessment. Collectively, these findings provided the first experimental evidence that SBF1 is overexpressed in CRC and suggest that it may represent an unrecognized component of phosphoinositide related pathways involved in colorectal tumorigenesis.</p>","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15598"},"PeriodicalIF":1.9,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13660866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149191323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alpha1-adrenoceptor antagonists block the positive chronotropic effect of 6-nitrodopamine in the isolated rat right atrium.","authors":"B L Alves, A J S Niederauer, J Britto-Júnior, J F Sobanski, T C C de Assis, D L Oliveira, G Said, G D Mendes, A S Pupo, S Marcondes, E Antunes, G De Nucci","doi":"10.1590/1414-431X2026e15550","DOIUrl":"10.1590/1414-431X2026e15550","url":null,"abstract":"<p><p>6-Nitrodopamine (6-ND) released from isolated rat atria produces potent positive chronotropic actions. Direct negative chronotropic responses to some selective α1-adrenoceptor (AR) antagonists like prazosin have been reported. Since α1-AR antagonists inhibit 6-ND-induced smooth muscle contractions, we have hypothesized that negative chronotropism induced by α1-AR antagonists reflects 6-ND blockade. This study investigated whether selective α1-AR antagonists affect 6-ND-induced positive chronotropism in isolated rat atria. Alfuzosin at 10 nM did not affect the atrial rate, but at 100 nM, it significantly reduced the atrial rate. This effect was not observed in L-NAME (100 mM)-treated preparations or in atria from rats chronically treated with L-NAME. Alfuzosin (100 nM) significantly reduced the 6-ND-induced positive chronotropism without affecting the chronotropic responses to noradrenaline, adrenaline, or dopamine. Similar results were obtained with doxazosin (1-10 nM), tamsulosin (10-100 nM), and prazosin (1-10 nM). Isoprenaline induced concentration-dependent increases in atrial rate, which were not significantly affected by any of these α1-AR antagonists. The results demonstrated that α1-AR antagonists reduced atrial basal rate only at concentrations affecting the positive chronotropic effect of 6-ND, and this reduction was not observed when NO synthesis was blocked, indicating that the negative chronotropic effect may involve 6-ND receptor blockade.</p>","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15550"},"PeriodicalIF":1.9,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13660870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149191312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative multi-omics analysis identifies CCDC69 as a marker associated with germinal center B-cell infiltration in TNBC: development and validation.","authors":"Jinjiao Li","doi":"10.1590/1414-431X2026e15616","DOIUrl":"10.1590/1414-431X2026e15616","url":null,"abstract":"<p><p>Although coiled-coil domain containing 69 (CCDC69) has been implicated in tumor biology, its role in triple-negative breast cancer (TNBC) and immune regulation remains unclear. Bulk transcriptomic data, single-cell RNA sequencing datasets, and spatial transcriptomics were used to investigate the role of CCDC69 in TNBC. Germinal center B-cell (GCB) signatures were quantified using single-sample gene set enrichment analysis (ssGSEA), and their associations with CCDC69 expression were assessed using correlation, ROC, and logistic regression analyses. An independent external TNBC cohort (GSE58812) was used to validate the association between CCDC69 expression, GCB infiltration, and survival outcomes. CCDC69 expression was significantly downregulated in breast cancer tissues but was associated with favorable survival outcomes in TNBC patients. CCDC69 expression positively correlated with multiple immune cell populations, particularly B cells and plasma cells. Single-cell RNA sequencing demonstrated that CCDC69 expression was enriched in B-lineage cells, especially GCB. Spatial transcriptomic analyses further showed that CCDC69-high regions were spatially co-localized with B-cell-rich and germinal center-like immune niches. In the TCGA TNBC cohort, CCDC69 expression was strongly associated with GCB infiltration and effectively distinguished GCB-high tumors. Logistic regression analysis confirmed that CCDC69 was independently associated with GCB enrichment. Findings were validated in the GSE58812 cohort. CCDC69 expression correlated with GCB-related signatures, showed good discriminative performance in identifying GCB-high tumors, and was associated with improved patient outcomes when combined with GCB enrichment. CCDC69 is closely associated with B-cell-mediated immune responses and germinal center niches in TNBC. Our findings suggest that CCDC69 may serve as a robust biomarker of immune activation and favorable prognosis.</p>","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15616"},"PeriodicalIF":1.9,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13660869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149191300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hematological indices as predictors of in-hospital adverse outcomes in patients with COVID-19.","authors":"E B Pordeus, N A Filgueira, C R P Soares, P S R de Araújo","doi":"10.1590/1414-431X2026e15557","DOIUrl":"10.1590/1414-431X2026e15557","url":null,"abstract":"<p><p>The SARS-CoV-2 pandemic required medical teams to make rational decisions with limited resources, especially in Brazil. Various diagnostic tools, clinical guidelines, and existing indices were adopted to predict the risk of death and other adverse outcomes. The objective of this study was to validate three hematological indices [neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR), and lymphocyte-monocyte ratio (LMR)] for predicting adverse hospital outcomes in patients with COVID-19 in an observational cohort at a hospital in northeastern Brazil between April 2020 and October 2021. Hematological indices were calculated based on admission blood counts and correlated with hospital outcomes: in-hospital death, invasive mechanical ventilation, and renal replacement therapy. The analysis determined the frequencies of outcomes, established index thresholds, and assessed their association and accuracy and factors associated with hospital survival. Intra-hospital outcomes reflected disease severity; 34.62% of patients required admission to the ICU, 26.21% invasive mechanical ventilation, 4.2% renal replacement therapy, and 21.04% died. Advanced age, ICU requirement, and higher NLR were associated with higher risk of death. Multivariate analysis identified NLR as the only index associated with worse prognosis, with a cutoff point of 5.24 at hospital admission, showing a sensitivity of 84.0% and accuracy of 86.9%. The negative predictive value of 95.52% was highly significant, assisting hospital management by focusing efforts on higher-risk patients. These findings highlight the predictive value of the NLR, PLR, and LMR indices as valuable markers for risk stratification in patients hospitalized with COVID-19, offering a broader view of prognosis and guiding more personalized management and care strategies.</p>","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15557"},"PeriodicalIF":1.9,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13660867/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149191354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Yo-yo intermittent recovery test, physiological markers of endurance performance, and buffering capacity in professional soccer players.","authors":"R C A Ferreira, V O Silvino, L V Vilhena, M A P Dos Santos, D M L do Prado","doi":"10.1590/1414-431X2026e15230","DOIUrl":"10.1590/1414-431X2026e15230","url":null,"abstract":"<p><p>The Yo-Yo Intermittent Recovery Test Level 1 (YYIR1) is widely recognized as a reliable tool for assessing intermittent endurance and aerobic capacity in team sports athletes. This study aims to verify the association between YYIR1 and physiological markers of endurance performance in professional soccer players, as well as the relationship between metabolic buffering capacity, metabolic acidosis tolerance, and YYIR1 total distance. Twenty-six professional soccer athletes (26.3±4.98 years, 76.4±8.96 kg, 178±6.52 cm, 54.1±4.85 mL·kg-1·min-1 V̇O2max) performed the YYIR1 and underwent a cardiopulmonary exercise test (CPET) on a treadmill in order to assess their ventilatory thresholds (aerobic threshold (AT), respiratory compensation point (RCP), and maximal oxygen consumption (V̇O2max)), buffering capacity, and tolerance to metabolic acidosis. Correlation analyses revealed that the YYIR1 distance was significantly associated with maximal aerobic speed (r=0.542, P=0.006) and V̇O2max (r=0.492, P=0.015), but not with AT or RCP. Position-specific differences were observed in relative acidosis tolerance capacity, with midfielders presenting higher values than defenders (P=0.018, η2=0.294), whereas no significant differences were noted for relative buffering or YYIR1 distance across positions. In conclusion, maximal aerobic speed and V̇O2max were the strongest predictors of YYIR1 performance in professional soccer players, outperforming submaximal thresholds such as AT and RCP. Moreover, positional differences in acidosis tolerance were identified, as midfielders demonstrated greater relative acidosis tolerance capacity compared to defenders.</p>","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15230"},"PeriodicalIF":1.9,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13660868/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149191302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application effects of information-based health management combined with knowledge-attitude-practice health education model in hypertensive patients.","authors":"Jiangwen Duan, Qitao Jin","doi":"10.1590/1414-431X2026e15576","DOIUrl":"10.1590/1414-431X2026e15576","url":null,"abstract":"<p><p>This study investigated the application effects of information-based health management combined with the knowledge-attitude-practice (KAP) health education model in hypertensive patients. Two hundred hypertensive patients were randomized into a control group or an intervention group. Patients in the control group received conventional health management and health education, whereas those in the intervention group received information-based health management plus the KAP health education model. The blood pressure control rate, pre- and post-management systolic and diastolic blood pressures, medication adherence, self-management abilities [assessed using the Hypertension Patient Self-Management Behavior Rating Scale (HPSMBRS)], health behaviors [examined using the Health-Promoting Lifestyle Profile II (HPLP-II)], quality of life [estimated using the Generic Quality of Life Inventory-74 (GQOLI-74)], and patient satisfaction with management were compared between the two groups. Following management, the blood pressure control rate in the intervention group was higher relative to the control group (P<0.05). Compared to pre-management levels, both groups showed noticeable reductions in systolic and diastolic blood pressures post-management, with lower values observed in the intervention group (P<0.05). Both groups exhibited increased medication adherence, self-management abilities, health behavior scores, and quality of life, with higher values in the intervention group (P<0.05). Patient satisfaction with management was higher in the intervention group (P<0.05). Information-based health management plus the KAP health education model effectively improved blood pressure control, medication adherence, self-management ability, health behaviors, and quality of life in patients with hypertension, with high patient satisfaction.</p>","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15576"},"PeriodicalIF":1.9,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13560477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148918512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of dextran sulfate sodium-induced colitis and associated mortality in BALB/c mice.","authors":"D E Molotla-Torres, M E Drago-Serrano, M Godínez-Victoria, R Oros-Pantoja, F Guzmán-Mejía","doi":"10.1590/1414-431X2026e15450","DOIUrl":"10.1590/1414-431X2026e15450","url":null,"abstract":"<p><p>Dextran sulfate sodium (DSS)-induced colitis in BALB/c mice has revealed underlying mechanisms of colitis pathology. This study aimed to optimize the DSS dosage to improve reproducibility while ensuring high survival rates. Mice were treated with 2 and 3% DSS solutions in drinking water daily for 7 days (days 1-7), followed by water (8-14 days). Disease activity index (DAI) and survival percentage were monitored over 14 consecutive days. On the last day, intestinal permeability was assessed by Evans blue assay, and colon length was measured. After sacrifice, colon samples were collected to evaluate mucopolysaccharide content and processed for histological analysis with hematoxylin-eosin (HE), periodic acid-Schiff (PAS), and Alcian blue (AB) staining. The results were analyzed to identify significant differences between groups, considering a P-value ≤0.05 as significant. Compared to the control group, DAI, inflammatory cell infiltrate, and intestinal permeability were higher, while colon length, body weight, and mucopolysaccharide content were lower in both DSS groups. Compared to the 2% DSS group, the 3% DSS group showed higher relative density of AB+ cells, higher DAI on days 7, 9, and 14, greater inflammatory infiltrate, and lower PAS+ cells. In addition, survival was lower in the 3% DSS group (57%) compared to the 2% DSS group (80%). According to these findings, 2% DSS provided higher survival and symptomatic colitis, making it the best choice for inducing DSS colitis in mice.</p>","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15450"},"PeriodicalIF":1.9,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13557328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148918488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical efficacy of uniportal and biportal endoscopic ULBD techniques in the treatment of lumbar spinal stenosis.","authors":"Penghui Ni, Xueguo Chen, Wenwen Huang","doi":"10.1590/1414-431X2026e15561","DOIUrl":"10.1590/1414-431X2026e15561","url":null,"abstract":"<p><p>This study aimed to compare the clinical efficacy of unilateral laminotomy for bilateral decompression (ULBD) performed using unilateral endoscopy (UE) versus unilateral biportal endoscopy (UBE) in patients with lumbar spinal stenosis (LSS). Sixty LSS patients were randomly assigned to a UE-ULBD group or a UBE-ULBD group (30 cases each). Intraoperative blood loss, operative time, time to first ambulation, length of hospital stay, and intraoperative/postoperative complications were recorded. Low back and leg pain were assessed using the visual analog scale (VAS), and functional status was evaluated using the Oswestry Disability Index (ODI) prior to surgery and at 3 and 6 months postoperatively. Quality of life was measured by the Swiss Spinal Stenosis (SSS) questionnaire preoperatively and at 6 months post-surgery. Surgical outcomes were evaluated using the modified MacNab criteria. No differences were found between groups for intraoperative blood loss, operative time, time to first ambulation, length of hospital stay, or intraoperative/postoperative complication rates (P>0.05). Compared with preoperative values, both groups showed notable reductions in VAS and ODI scores at 3 and 6 months postoperatively (P<0.05). At 6 months postoperatively, all SSS dimension scores were significantly improved in both groups (P<0.05), and no intergroup differences were detected (P>0.05). The modified MacNab excellent-to-good rates were 93.33% in the UBE-ULBD group and 90% in the UE-ULBD group, with no significant between-group difference (P>0.05). UE-ULBD and UBE-ULBD represent reliable minimally invasive options for the surgical management of LSS.</p>","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15561"},"PeriodicalIF":1.9,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13557330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148918485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting diagnostic gene biomarkers associated with immune infiltration in patients with diabetes.","authors":"Juan Feng, Tianhang Zhao, Sien Lai, Yiyang Huang, Yufen Xie, Guojun Chen","doi":"10.1590/1414-431X2026e15577","DOIUrl":"10.1590/1414-431X2026e15577","url":null,"abstract":"<p><p>Diabetes is a global public health problem with various complications, which can lead to disability and mortality. This study identified potential diagnostic markers for diabetes and explored the immunometabolic mechanisms in the pathological process. The gene expression of 17 diabetes cases and 16 normal controls were obtained from the Gene Expression Omnibus (GEO) database. The \"limma\" package was employed for screening differentially expressed genes (DEGs). Gene functions and enriched pathways of DEGs were analyzed via Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Candidate key genes were screened using the least absolute shrinkage and selection operator (LASSO) regression model and support vector machine recursive feature elimination (SVM-RFE) analysis. The diagnostic effectiveness of identified markers was further verified via the receiver operating characteristic (ROC) curve. The compositional patterns of immune cell infiltration and signaling pathway enrichment associated with key genes were explored via single sample Gene Set Enrichment Analysis (ssGSEA) and GSEA analysis, respectively. Possible miRNAs interacting with key genes were predicted via miRcode database. B2M, FTL, SH3BGRL3, and SOD2 were recognized as diagnostic markers for diabetes based on LASSO regression and the support vector machine recursive feature elimination (SVM-RFE) feature selection algorithm. Analysis of immune cell infiltration demonstrated that the four key genes were related to B cells, neutrophils, macrophages, and CD8+ T cells. The diagnostic value of B2M, FTL, and SOD2 for diabetes was higher than that of SH3BGRL3 according to the ROC curve. Validation experiments indicated that the mRNA expression of B2M and FTL was increased in liver tissues of diabetic mice. B2M and FTL can act as diagnostic markers for diabetes and contribute to new understandings of the disease's molecular mechanisms.</p>","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15577"},"PeriodicalIF":1.9,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13557331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148918508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diet enriched in saturated fatty acids alters mean arterial blood pressure and fat metabolism but not autonomic balance in rats.","authors":"T H G Rodrigues, A P F C Arcoverde-Mello, A C Simões-Alves, G M da Silva, T R L A Lima, M P Fernandes, V O Nogueira, A G Wanderley, J H Costa-Silva","doi":"10.1590/1414-431X2026e15215","DOIUrl":"10.1590/1414-431X2026e15215","url":null,"abstract":"<p><p>This study aimed to evaluate the effects of a diet rich in saturated fatty acids on fat metabolism and oxidative damage and its repercussions on arterial blood pressure and autonomic balance. During pregnancy and lactation, primiparous Wistar rats received a standard laboratory diet. After weaning, the pups were divided into two groups according to their diet: a normolipidic diet (Control group, C) and a high-fat diet (Hyperlipidic group, HL). Food consumption, weight development, serum biochemical parameters, arterial blood pressure, and heart rate were evaluated. Adipose tissue was collected for oxidative stress measurements at 90 days of life (d). The HL group had a greater intake of lipids, greater abdominal circumference at 30 d, and increased oxidative stress in adipose tissue. Epididymal adipose tissue showed increased activity of fatty acid synthase (FAS) and decreased activity of β-hydroxyacyl-CoA dehydrogenase (β-HAD). These effects were associated with high mean arterial pressure but were not associated with changes in cardiovascular variability in time and frequency domains at 90 days. Thus, the intake of a diet rich in saturated fatty acids for up to 90 days may be related to a greater induction of oxidative stress in visceral adipose tissue, preceding the development of autonomic dysfunction and arterial hypertension in older individuals.</p>","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15215"},"PeriodicalIF":1.9,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13557329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148918475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}