Brazilian Journal of Medical and Biological Research最新文献_第3页

Nicotinamide mononucleotide alleviates the streptozotocin-mediated ferroptosis of islet β-cells via the Nrf2/GPX4 pathway. 烟酰胺单核苷酸通过Nrf2/GPX4途径减轻链脲佐菌素介导的胰岛β-细胞铁凋亡。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-08-17 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2026e15295

Decheng Lu, Jianli Huang, Jinyue Meng, Jiayu Luo, Xuemei Huang

{"title":"Nicotinamide mononucleotide alleviates the streptozotocin-mediated ferroptosis of islet β-cells via the Nrf2/GPX4 pathway.","authors":"Decheng Lu, Jianli Huang, Jinyue Meng, Jiayu Luo, Xuemei Huang","doi":"10.1590/1414-431X2026e15295","DOIUrl":"https://doi.org/10.1590/1414-431X2026e15295","url":null,"abstract":"The effects of nicotinamide mononucleotide (NMN) on streptozotocin (STZ)-induced islet β-cell dysfunction and the underlying mechanisms have not been fully elucidated. This study investigated the ameliorative effect and mechanism of NMN on islet β-cell dysfunction. NMN significantly increased the INS-1 cell viability following treatment with either STZ or erastin and effectively mitigated STZ-induced cell injury. Upon exposure to STZ, INS-1 cells exhibited significantly elevated intracellular Fe2+ levels, significantly increased reactive oxygen species (ROS) levels, and significantly decreased in the activities of superoxide dismutase (SOD) and glutathione (GSH). The levels of lipid peroxidation (LPO) and malondialdehyde (MDA) were substantially increased, and the mitochondrial membrane potential significantly decreased. Transmission electron microscopy further revealed mitochondrial shrinkage, rupture of the mitochondrial membrane, and disintegration of mitochondrial cristae. NMN markedly reduced intracellular iron accumulation in INS-1 cells treated with STZ. NMN subsequently attenuated intracellular ROS, LPO, and MDA levels while restoring GSH and SOD activities in STZ-exposed cells. Moreover, NMN restored the mitochondrial membrane potential and reversed the STZ-induced pathological changes in mitochondrial morphology. Compared with STZ treatment, ferrostatin-1 treatment significantly upregulated Nrf2 and GPX4 expression. In contrast, compared with STZ treatment, erastin treatment suppressed Nrf2 and GPX4 expression. Notably, compared with STZ or erastin treatment, cotreatment with NMN resulted in a further reduction in Keap1 expression and a significant upregulation of Nrf2 and GPX4. NMN may alleviate STZ-induced INS-1 cell ferroptosis by activating the Nrf2/GPX4 pathway, providing a theoretical basis for the treatment of diabetes mellitus.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15295"},"PeriodicalIF":1.9,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13480942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148788081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endocannabinoid system attenuates emotional stress-induced orofacial musculoskeletal pain in rats. 内源性大麻素系统减轻大鼠情绪应激诱导的口面部肌肉骨骼疼痛。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-08-07 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2026e15331

S S Silveira, B W F Alves, M R P Lisboa, J J R Nogueira, K L S de Menezes, A F Pereira, R F Lima, D V Gondim, M L Vale

{"title":"Endocannabinoid system attenuates emotional stress-induced orofacial musculoskeletal pain in rats.","authors":"S S Silveira, B W F Alves, M R P Lisboa, J J R Nogueira, K L S de Menezes, A F Pereira, R F Lima, D V Gondim, M L Vale","doi":"10.1590/1414-431X2026e15331","DOIUrl":"10.1590/1414-431X2026e15331","url":null,"abstract":"Orofacial musculoskeletal pain (OMP) is a common feature of temporomandibular disorders (TMD), a group of conditions affecting the temporomandibular joint, chewing muscles, and associated structures. The etiology of muscular TMD is currently understood within a biopsychosocial model of pain, highlighting the complexity related to OMP. In this context, the objective of this study was to investigate the OMP induced by psychological/emotional stress (ES) in rats, regarding the role of the endocannabinoid system (ECS) through CB1 and CB2 receptors. Male Wistar rats were divided into Control and ES groups. OMP was induced by ES using the communication box model and evaluated through the mechanical threshold in masticatory muscles. c-Fos, CB1, and CB2 immunostaining was evaluated in the trigeminal ganglion (TG) and in the trigeminal nucleus caudalis (Sp5C). The selective CB1 or CB2 antagonist (AM251 or AM630, respectively) or the cannabinoid receptor non-selective agonist (WIN55,212-2) were administered to both groups, and the OMP was evaluated. The mechanical sensitivity of the masticatory muscles increased in the ES group, accompanied by increased c-Fos expression in the TG and Sp5C. AM251 and AM630 increased mechanical sensitivity, while WIN55,212-2 decreased the OMP. Furthermore, ES increased CB1 density in the TG and elevated both CB1 and CB2 in the Sp5C. Additionally, CB2 was increased in the masseter muscle. Thus, cannabinoid receptors played a protector role in OMP caused by ES, indicating that cannabinoid drugs or the modulation of the ECS may represent a promising approach for the treatment of OMP frequently observed in patients with TMD.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15331"},"PeriodicalIF":1.9,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13450814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148719601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The flavonoid isoliquiritigenin promotes lifespan extension of Caenorhabditis elegans through the transcription factor DAF-16 and aquaporin-2. 类黄酮异尿原素通过转录因子DAF-16和水通道蛋白-2促进秀丽隐杆线虫的寿命延长。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-08-07 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2025e14996

Xuan Zhao, Limei Ren, Dandan Gu, Yasen Yao, Shuo Wang, Jiali Gao, Yonghao Qi

{"title":"The flavonoid isoliquiritigenin promotes lifespan extension of Caenorhabditis elegans through the transcription factor DAF-16 and aquaporin-2.","authors":"Xuan Zhao, Limei Ren, Dandan Gu, Yasen Yao, Shuo Wang, Jiali Gao, Yonghao Qi","doi":"10.1590/1414-431X2025e14996","DOIUrl":"10.1590/1414-431X2025e14996","url":null,"abstract":"Aging is associated with the accumulation of oxidative damage. Isoliquiritigenin (ISL), a natural flavonoid, exhibits antioxidant properties, but its effect on lifespan and the underlying mechanisms remains incompletely understood. This study aimed to investigate whether ISL extends lifespan in Caenorhabditis elegans through the transcription factor DAF-16 and the aquaporin AQP-2. We employed lifespan assays, stress resistance tests, intracellular ROS measurement, osmotic water permeability assays in Xenopus oocytes, genetic manipulation (using mutants and RNAi), quantitative real-time PCR, and fluorescence microscopy to assess DAF-16 localization and target gene expression. ISL extended the mean lifespan of wild-type C. elegans by 15.35% at 20 μM (P<0.01). It enhanced resistance to oxidative stress (36.54% higher survival under paraquat) and heat shock (20.56% higher survival at 37°C), and reduced intracellular ROS levels. Mechanistically, ISL increased DAF-16 nuclear translocation by 44.3% (P=0.003), and upregulated the expression of its target genes, including sod-3 (3.2-fold, P=0.008) and aqp-2 (2.9-fold, P=0.012). ISL also increased the osmotic water permeability of Xenopus oocytes by 1.7-fold (P=0.007), and the effect was abolished by the aquaporin inhibitor HgCl2. Genetic ablation of aqp-2 nullified ISL-induced lifespan extension (P=0.65 vs control) and ROS reduction. Crucially, aqp-2 RNAi suppressed ISL-driven DAF-16 nuclear accumulation and sod-3 expression, establishing a feedforward loop. ISL extended lifespan and enhanced stress resistance in C. elegans by activating a DAF-16/AQP-2 regulatory module, thereby linking water homeostasis to the transcriptional control of aging.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e14996"},"PeriodicalIF":1.9,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13450824/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148721164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between sedentary behavior and daily step count with physical performance of community-dwelling older adults participating in an exercise program interrupted by the COVID-19 pandemic: a cross-sectional study in Brazil. 在巴西进行的一项横断面研究中，参与因COVID-19大流行而中断的锻炼计划的社区老年人的久坐行为和每日步数与身体表现之间的关系。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-08-07 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2026e14959

V R S Santos, G Cassemiliano, A C S Farche, S Lee, L B Message, P G Rossi, A C M Takahashi

{"title":"Association between sedentary behavior and daily step count with physical performance of community-dwelling older adults participating in an exercise program interrupted by the COVID-19 pandemic: a cross-sectional study in Brazil.","authors":"V R S Santos, G Cassemiliano, A C S Farche, S Lee, L B Message, P G Rossi, A C M Takahashi","doi":"10.1590/1414-431X2026e14959","DOIUrl":"10.1590/1414-431X2026e14959","url":null,"abstract":"One of the effects of the COVID-19 pandemic was the interruption of exercise programs for older adults, significantly impacting their physical performance (PP). Few studies have identified the effects of the interruption of exercise programs on the PP of older adults, and none have assessed the relationship between this outcome and objectively measured daily step count and sedentary behavior (SB). This study assessed the association between SB and daily step count with PP in 42 community-dwelling older adults (73.86±6.78 years old, 88.10% female) after an 18-month interruption of an exercise program due to the COVID-19 pandemic. SB and daily step count were measured using the activPAL3™ micro accelerometer, while PP was assessed through the 30-s sit-to-stand test, handgrip strength test, and Timed Up and Go (TUG) test. Data were analyzed using Pearson correlation test and linear regression model. Results showed that daily step count was significantly correlated with PP (r=0.38 to 0.42). Regression analysis revealed that a mean increment of 1,000 steps/day was associated with a mean increase of 0.31 repetitions in the sit-to-stand test, 0.89 kgF in handgrip strength, and a mean reduction of 0.30-s in TUG time. Additionally, older adults who reached approximately 9,325 steps/day demonstrated better PP outcomes compared to those who accumulated a lower number of daily steps. However, SB was not significantly associated with PP (P>0.05). These findings suggest that accumulating high amounts of daily steps may help mitigate declines in PP when structured exercise programs are interrupted.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e14959"},"PeriodicalIF":1.9,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13450813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148720189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of sex and gonadal hormones on pain perception in a neuropathic pain model in old rats. 性激素和性激素对老龄大鼠神经性疼痛模型疼痛感知的影响。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-08-07 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2026e15116

C C A Palmeira, I D G Gaudêncio, L O Contiero, J O Barbosa Neto, D C Munhoz, H A Ashmawi

{"title":"Influence of sex and gonadal hormones on pain perception in a neuropathic pain model in old rats.","authors":"C C A Palmeira, I D G Gaudêncio, L O Contiero, J O Barbosa Neto, D C Munhoz, H A Ashmawi","doi":"10.1590/1414-431X2026e15116","DOIUrl":"10.1590/1414-431X2026e15116","url":null,"abstract":"Despite growing evidence that nociceptive stimuli are processed and modulated by distinct neural mechanisms depending on biological sex and aging, these factors remain underrepresented in experimental and clinical pain research. Most preclinical studies continue to rely on young male rodents, overlooking potential differences in neuropathic pain perception between sexes and age groups. Understanding these variations is critical for advancing pain management strategies. Aged rats (22 months old) of both sexes were divided into three groups: orchiectomized (Orch) males, non-orchiectomized (Non-orch) males, and females. The animals underwent L5 spinal nerve ligation and were assessed for mechanical allodynia on the plantar surface of their left hind paw before nerve ligation and on days 7, 14, 21, and 28 post-ligation. By day 14, Non-orch males showed a significantly higher withdrawal threshold than Orch males, with an average difference of 8.46 g (P<0.005), which remained significant and increased over time, peaking at day 28, with 13.07 g (P<0.0006). By day 28, Non-orch males also showed greater mechanical allodynia than females, with an estimated mean difference of approximately 8 g (P=0.038). These findings suggest that male gonadal hormones mitigate neuropathic pain-induced mechanical allodynia. The study underscores the role of testosterone in pain modulation and highlights the need for further research on the interplay between hormonal regulation, aging, and neuropathic pain mechanisms.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15116"},"PeriodicalIF":1.9,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13450812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148720729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic value of preoperative CCL26 serum levels in the surgical treatment of chronic rhinosinusitis. 术前血清CCL26水平对慢性鼻窦炎手术治疗的预后价值。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-08-07 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2026e14736

Xudong Wang, Shunan Liu, Jichuan Chen, Wanting Zeng, Jianguo Jiang

{"title":"Prognostic value of preoperative CCL26 serum levels in the surgical treatment of chronic rhinosinusitis.","authors":"Xudong Wang, Shunan Liu, Jichuan Chen, Wanting Zeng, Jianguo Jiang","doi":"10.1590/1414-431X2026e14736","DOIUrl":"10.1590/1414-431X2026e14736","url":null,"abstract":"Eosinophilic chronic rhinosinusitis (CRS), characterized by prominent eosinophil infiltration, has the highest postoperative recurrence rate among CRS subtypes. Given the critical role of eotaxins in CRS pathogenesis, this study aimed to identify eotaxins associated with eosinophilic CRS and evaluate their correlation with surgical outcomes. Transcriptomic data from GSE36830 revealed 125 differentially expressed genes (49 up-regulated, 76 down-regulated) in CRSwNP compared to CRSsNP, with significant enrichment of eosinophil and dendritic cell infiltration pathways. Functional analysis identified chemotaxis and cell migration as key dysregulated processes in CRSwNP. Among eotaxins, CCL26 and CCL13 emerged as hub molecules from the intersection of these dysregulated pathways. In a clinical cohort of 115 CRS patients, preoperative serum CCL26 and CCL13 levels were significantly elevated in CRSwNP compared to CRSsNP (P<0.001). ROC analysis demonstrated strong diagnostic power for nasal polyps (CCL26: AUC=0.895; CCL13: AUC=0.830). Higher preoperative CCL26 levels predicted poorer symptom improvement post-surgery (standardized β=-0.564, P<0.001). In conclusion, preoperative serum CCL26 serves as both a diagnostic biomarker for CRSwNP and a prognostic indicator for surgical outcomes, with elevated levels associated with reduced symptom improvement.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e14736"},"PeriodicalIF":1.9,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13450811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148721697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Home death versus hospital death: a retrospective analysis of patients in palliative care. 家庭死亡与医院死亡：姑息治疗患者的回顾性分析

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-07-24 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2026e15243

R D Bittencourt, S Krasilcic, I M da Silva, M K Koike

{"title":"Home death versus hospital death: a retrospective analysis of patients in palliative care.","authors":"R D Bittencourt, S Krasilcic, I M da Silva, M K Koike","doi":"10.1590/1414-431X2026e15243","DOIUrl":"https://doi.org/10.1590/1414-431X2026e15243","url":null,"abstract":"Respecting the patient's preferred place of death is a key indicator of the quality of palliative care. However, clinical factors may influence this outcome. This study aimed to compare the clinical profiles, symptom burden, and medical interventions of patients who died at home versus those who died in an inpatient setting (hospital) within the context of a Brazilian public healthcare institution. A retrospective study was conducted involving 227 patients who were followed by the Palliative Care Team at the Instituto de Assistência Médica ao Servidor Público Estadual (IAMSPE). Sociodemographic and clinical variables, symptom control, and pharmacological interventions were analyzed using generalized linear mixed models (GLMMs). Of these patients, 59.47% (n=135) died in the hospital ward and 40.53% (n=92) died at home. No statistically significant differences were observed between age or sex and place of death. Hospitalized patients presented with a greater number of symptoms, although no significant differences were found for pain and dyspnea. Most of the patients also had multiple metastases. The use of oxygen was significantly higher in the inpatient setting, while oral and enteral feeding was more feasible at home. Although the total number of medications administered was higher in the hospital, there were no significant differences in the use of morphine and midazolam between the groups. In conclusion, clinical severity was a key factor influencing hospitalization until death. Despite equivalent symptom control between settings, expanding access to structured home-based palliative care could enable patients to experience end-of-life care aligned with their preferences.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15243"},"PeriodicalIF":1.9,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148618324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of membrane RANKL production in osteoblasts mediates curcumin-inhibited osteoclastogenesis. 抑制成骨细胞膜RANKL生成介导姜黄素抑制的破骨细胞生成。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-07-10 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2026e15332

Tingwei Gao, Tie Ke, Zhanhao Xiao, Xi Gao

{"title":"Inhibition of membrane RANKL production in osteoblasts mediates curcumin-inhibited osteoclastogenesis.","authors":"Tingwei Gao, Tie Ke, Zhanhao Xiao, Xi Gao","doi":"10.1590/1414-431X2026e15332","DOIUrl":"10.1590/1414-431X2026e15332","url":null,"abstract":"Curcumin inhibits osteoclastogenesis and mitigates osteoporosis. Our data demonstrated that curcumin decreases membrane-bound RANKL (mRANKL) levels on osteoblasts. Notably, mRANKL exerts a more prominent role in osteoclastogenesis than its soluble counterpart (sRANKL). This study aimed to explore the role of RANKL membrane localization in curcumin-regulated osteoclastogenesis. We primarily investigated the effect of curcumin on mRANKL expression in osteoblasts in vitro and in vivo. Using fluorescence-activated cell sorting (FACS) for RANKL and co-culture experiments of osteoclast precursors (OCPs) with osteoblasts, we further analyzed the association between curcumin's indirect inhibition of osteoclast differentiation and mRANKL production. Finally, we explored the involvement of matrix metalloproteinase 14 (MMP14) in curcumin-mediated regulation of mRANKL levels. The results showed that curcumin significantly reduced mRANKL expression in osteoblasts. Curcumin also abrogated the upregulated RANKL levels in osteoblasts of Tg-hRANKL transgenic mice. Co-culture assays revealed that curcumin exerted the weakest inhibitory effect on osteoclast differentiation when OCPs were co-cultured with mRANKL-negative osteoblasts. Additionally, curcumin increased MMP14 expression in osteoblasts and enhanced the MMP14-RANKL interaction. Importantly, silencing MMP14 in osteoblasts reversed curcumin-inhibited mRANKL levels and osteoclast differentiation. Collectively, our findings indicated that curcumin inhibited mRANKL production in osteoblasts, which contributes to its therapeutic effect on osteoclastic osteoporosis.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15332"},"PeriodicalIF":1.9,"publicationDate":"2026-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13362958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148444457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Capsinoids treatment reduces steatosis with consequent attenuation in the progression of metabolic dysfunction-associated steatotic liver disease in obese rats. 在肥胖大鼠中，辣椒素治疗可以减少脂肪变性，从而减少代谢功能障碍相关的脂肪变性肝病的进展。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-07-10 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2026e15127

L M Simmer, F M Nunes, K C C Santos, D A G Saiz, K O Miranda, E R Cordeiro, J N Boeloni, D S Bocalini, A S Leopoldo, A P Lima-Leopoldo

{"title":"Capsinoids treatment reduces steatosis with consequent attenuation in the progression of metabolic dysfunction-associated steatotic liver disease in obese rats.","authors":"L M Simmer, F M Nunes, K C C Santos, D A G Saiz, K O Miranda, E R Cordeiro, J N Boeloni, D S Bocalini, A S Leopoldo, A P Lima-Leopoldo","doi":"10.1590/1414-431X2026e15127","DOIUrl":"10.1590/1414-431X2026e15127","url":null,"abstract":"Among the morbidities triggered by obesity, metabolic dysfunction-associated steatotic liver disease (MASLD) stands out, with its progression resulting from the deposition of lipid molecules in the liver. There is a growing interest in natural/functional foods as an alternative for improving health, as well as for the treatment and prevention of diseases. Capsinoids (Cap) - bioactive compounds present in peppers of the genus Capsicum annuum - have been studied for promoting loss of adiposity and increased caloric expenditure. Therefore, this study aimed to investigate the effects of Cap on liver parameters in obese (Ob) rats induced by a high-fat diet (HFD). Male Wistar rats were initially randomized into two groups: standard diet (SD) and HFD. The protocol lasted 27 weeks, including 19 weeks of induction and maintenance of obesity and 8 weeks of Cap treatment. At week 19, the HFD rats were redistributed into the Obese (Ob) and Obese capsinoids (ObCap) groups, and the ObCap group was supplemented daily with chronic Cap treatment by orogastric gavage (10 mg Cap/kg daily). Cap treatment was not effective in improving adiposity and inflammatory parameters of obesity, although it reduced ghrelin, cholesterol, and hepatic fat accumulation, and attenuated MASLD progression, which may be promising for combating chronic diseases related to lipid metabolism.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15127"},"PeriodicalIF":1.9,"publicationDate":"2026-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13362892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148444390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electroacupuncture alleviates Parkinson's disease-related pain by inhibiting microglial NLRP3-ASC inflammasome in the amygdala. 电针通过抑制杏仁核小胶质NLRP3-ASC炎性体减轻帕金森病相关疼痛。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-07-10 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2026e15248

Xiao-Wei Wang, Ben-Fan Zhu, Ying Shi, A-Long Xia, Ai-Ling Wang, Jing-Jing Peng, Si-Huan Zhu, Zhi-Lai Yang, Jun Li

{"title":"Electroacupuncture alleviates Parkinson's disease-related pain by inhibiting microglial NLRP3-ASC inflammasome in the amygdala.","authors":"Xiao-Wei Wang, Ben-Fan Zhu, Ying Shi, A-Long Xia, Ai-Ling Wang, Jing-Jing Peng, Si-Huan Zhu, Zhi-Lai Yang, Jun Li","doi":"10.1590/1414-431X2026e15248","DOIUrl":"10.1590/1414-431X2026e15248","url":null,"abstract":"This study aimed to investigate the role of microglia and NOD-like receptor protein 3 (NLRP3) inflammasome-mediated neuroimmune pathways in the analgesic effects of electroacupuncture (EA) in a mouse model of Parkinson's disease (PD). Male C57BL/6 mice (8 weeks old) were randomly assigned to the control, PD model, and PD + EA groups. PD was induced by intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), while control mice received saline. EA was administered to the motor cortex once daily for five consecutive days in the PD + EA group, whereas PD model mice were restrained without receiving EA stimulation. Behavioral assessments were performed to evaluate motor function and nociceptive sensitivity. Immunohistochemistry, immunofluorescence, and western blot analyses were used to quantify tyrosine hydroxylase (TH), ionized calcium-binding adapter molecule 1 (Iba-1), NLRP3 inflammasome components, and inflammatory cytokines in key brain regions. Compared with control mice, PD model mice showed reduced motor performance and heightened nociceptive sensitivity, accompanied by a decrease in TH-positive neurons and an increase in Iba-1-positive microglia in both the substantia nigra and amygdala. EA significantly improved motor performance and increased pain thresholds. Moreover, EA preserved TH-positive neurons, suppressed microglial activation, and downregulated the expression of NLRP3, ASC, caspase-1, interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in the amygdala. These findings suggest that EA alleviates PD-related pain, possibly by modulating microglial activation and NLRP3 inflammasome signaling in the amygdala, thereby reducing neuroinflammation.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15248"},"PeriodicalIF":1.9,"publicationDate":"2026-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13362890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148444516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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