Biointerface Research in Applied Chemistry最新文献_第6页

Designing of Chimeric Vaccine against Canine Distemper Virus Targeting Hemaglutanin Protein 以血凝素蛋白为靶点的犬瘟热病毒嵌合疫苗的设计

Biointerface Research in Applied Chemistry Pub Date : 2022-09-17 DOI: 10.33263/briac134.347

{"title":"Designing of Chimeric Vaccine against Canine Distemper Virus Targeting Hemaglutanin Protein","authors":"","doi":"10.33263/briac134.347","DOIUrl":"https://doi.org/10.33263/briac134.347","url":null,"abstract":"The canine distemper virus is highly contagious and affects dogs' respiratory systems. The virus belongs to the paramyxoviridae family and order Mononegavirales. This class of viruses contains a negative-strand RNA. This virus also affects raccoons, foxes, and other animals. The current study aims to design a vaccine against the virus employing reverse vaccinology. The target candidate for the vaccine design is a surface protein called Hemagglutinin. Viral hemagglutinin protein sequences were retrieved from the Uniprot database, and conserved regions were identified. Possible B-cell epitope regions were identified using the ABCpred server. These epitopes were analyzed for allergenic and antigenic properties using the Allergen FP server and VaxiJen v2.0 server. The epitopes, which were antigenic and non-allergenic, were screened for T cell epitopes using NetMHC and NetMHC2 servers. The toxicity of the selected peptides was evaluated using the Toxinpred server. The epitopes were further screened for transmembrane helices and signal peptides employing TMHMM v. 2.0 and SignalP 4.1 servers, respectively. The epitopes were then checked for the parameters using the ProtParam tool. Finally, the solubility of the epitopes was determined using the SOLPro server. Using the selected epitopes, a chimeric vaccine construct was constructed with the peptides by linking the peptides with the GPGPG linker to the cholera toxin subunit B. The chimeric vaccine was modeled using the Robetta server, and codon optimization of the construct was performed using the JCAT tool.","PeriodicalId":9026,"journal":{"name":"Biointerface Research in Applied Chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45514664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Annotated Review on Various Biological Activities of Quinoline Molecule 喹啉分子的多种生物活性评述

Biointerface Research in Applied Chemistry Pub Date : 2022-09-17 DOI: 10.33263/briac134.355

引用次数: 1

Spike Protein Potential Receptors Study: Comparative Computational Analysis Approach on SARS-CoV-2 -AC2/CD147 Complexes 刺突蛋白电位受体研究:SARS-CoV-2 -AC2/CD147复合物的比较计算分析方法

Biointerface Research in Applied Chemistry Pub Date : 2022-09-17 DOI: 10.33263/briac134.351

A. Makhloufi, R. Ghemit, M. El Kolli

引用次数: 1

Reno-protective Effect of Citrus sinensis by Regulating Antioxidant Capability and Gene Expression in Adenine-Induced Chronic Kidney in Rats 柑桔通过调节腺嘌呤诱导的大鼠慢性肾脏的抗氧化能力和基因表达对肾的保护作用

Biointerface Research in Applied Chemistry Pub Date : 2022-09-17 DOI: 10.33263/briac134.349

{"title":"Reno-protective Effect of Citrus sinensis by Regulating Antioxidant Capability and Gene Expression in Adenine-Induced Chronic Kidney in Rats","authors":"","doi":"10.33263/briac134.349","DOIUrl":"https://doi.org/10.33263/briac134.349","url":null,"abstract":"This study was performed to evaluate the reno-protective and antioxidant effects of aqueous Citrus sinensis (orange) peel extract in male Wistar rats administrated adenine. Adenine was administrated to male Wistar rats at a dose of 0.75 percent w/w in feed daily for four weeks. Rats previously fed adenine were treated with orange peel extract at a dose of 40 mg/kg BW/day by oral gavage during the same period of adenine administration. The results indicate that rats fed adenine- with aqueous orange peel extract significantly decreased urea, creatinine, and uric acid concentrations in the blood. The elevated kidney lipid peroxidation product, the lowered glutathione concentration, and the suppressed antioxidant enzyme activities were significantly improved. At the same time, the expressions of heme-oxygenase1 (HO-1), wnt7a, and β-catenin genes were actively regulated. Histological changes in the kidney were represented by congestion, hypertrophied glomerulus, vacuolization of the endothelial cells lining the glomerular tuft, and interstitial nephritis in adenine-administrated rats also remarkably improved. In conclusion, administering orange peel aqueous extract with adenine reduces the signs of adenine toxicity on renal function by enhancing the antioxidant defense system.","PeriodicalId":9026,"journal":{"name":"Biointerface Research in Applied Chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42724767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial Application of Carpathian Clinoptilolite as Cetylpyridinium Carrier 喀尔巴阡斜发沸石作为十六烷基吡啶载体的抗菌应用

Biointerface Research in Applied Chemistry Pub Date : 2022-09-17 DOI: 10.33263/briac134.348

引用次数: 1

A Molecularly Imprinted Polymer@PtNPs/MoS2-Based Electrochemical Platform for Sensing Glycated Albumin Concentration on the Screen-Printed Electrode (SPE) 基于分子印迹Polymer@PtNPs/ mos2的丝网印刷电极糖化白蛋白浓度检测电化学平台

Biointerface Research in Applied Chemistry Pub Date : 2022-09-17 DOI: 10.33263/briac134.352

{"title":"A Molecularly Imprinted Polymer@PtNPs/MoS2-Based Electrochemical Platform for Sensing Glycated Albumin Concentration on the Screen-Printed Electrode (SPE)","authors":"","doi":"10.33263/briac134.352","DOIUrl":"https://doi.org/10.33263/briac134.352","url":null,"abstract":"A majority of the population is affected by diabetes worldwide. The delay in detection leads to serious conditions such as cardiovascular disease, neuropathy, and others. To avoid severe consequences, it needs to be detected at the early stages. Various methods and techniques have already been introduced commercially to detect diabetes. But still, there are various limitations to these traditional methods (ion-exchange chromatography, high-performance liquid chromatography (HPLC), thiobarbituric acid (TBA) assay, boronate affinity chromatography) such as they require more time for operation; also, they are expensive and need expertise for operation. These limitations can be overcome with the application of biosensors integrated with nanomaterials and imprinting techniques. This presented study describes the development of an electrochemical biosensing platform for determining the concentration of glycated albumin. The biosensor was developed using the molecularly imprinting technique to enhance the specificity, stability, and selectivity. Further, to enhance the electrode's conductivity, surface area, and biocompatibility, the sensing platform was modified with Molybdenum disulfide (MoS2) nanosheets and Platinum nanoparticles (Pt NPs). Furthermore, the presented electrode was evaluated with electrochemical measurements. The biosensor exhibits a detection limit as low as 0.34 nM. Also, it operates in a dynamic concentration range from 0.34 nM to 700 μM. The actual working range was divided into lower (0.34 nM to 35 μM) and higher (200 to 700 μM).","PeriodicalId":9026,"journal":{"name":"Biointerface Research in Applied Chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43073674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Exploration of the Electronic, Spectroscopic Properties, and Bonding of an Antimalarial Drug of Chromium Arene–Quinoline Half Sandwich Complex in Aqueous Solution: A PCM Investigation 水溶液中抗疟药物铬芳烃-喹啉半夹层配合物的电子、光谱性质和键合的探索:PCM研究

Biointerface Research in Applied Chemistry Pub Date : 2022-09-12 DOI: 10.33263/briac134.313

引用次数: 0

Rosmarinus Officinalis L, Eriobotrya Japonica and Olea Europaea L Attenuate Adipogenesis in 3T3-L1-Derived Adipocytes and Inflammatory Response in LPS-Induced THP-1-Derived Macrophages 迷迭香、枇杷和油橄榄可减弱3t3 - l1来源的脂肪细胞的脂肪生成和lps诱导的thp -1来源的巨噬细胞的炎症反应

Biointerface Research in Applied Chemistry Pub Date : 2022-09-11 DOI: 10.33263/briac134.343

{"title":"Rosmarinus Officinalis L, Eriobotrya Japonica and Olea Europaea L Attenuate Adipogenesis in 3T3-L1-Derived Adipocytes and Inflammatory Response in LPS-Induced THP-1-Derived Macrophages","authors":"","doi":"10.33263/briac134.343","DOIUrl":"https://doi.org/10.33263/briac134.343","url":null,"abstract":"This study aims to in vitro evaluate the impact of three traditionally used anti-obesity medicinal plant extracts on inflammatory mediators in the THP-1 cell line and adipocyte differentiation and fat accumulation in the 3T3-L1 cell line. After extract preparation, an in vitro evaluation of cell viability using MTT on THP-1 and 3T3-L1, NO production levels using Griess reagent and cytokines production in THP-1 cells, and adipogenicity evaluation using Oil Red O on 3T3-L1 cells were performed. O. europaea showed the highest cytostatic on THP-1 cells with IC50 of 290.6 µg/mL, while R. officinalis was the highest on 3T3-L1 cells with IC50 of 486.6 µg/mL. All extracts significantly reduced dose-dependently nitric oxide (NO) production by THP-1-derived macrophages from 80 µM to control levels after treatment with 125 µg/mL. Similarly, all extracts decreased the levels of TNF-α and IL-6 production dose-dependently, with the highest effects reached at 250 µg/mL. all extracts increased the production levels of IL-10 from 32 pg/mL to 106.8 pg/mL, 83.5 pg/mL and 87 pg/mL at 125µg/mL, respectively. After treatment with plant extracts, the adipocyte differentiation and fat accumulation in 3T3-L1 were reduced to 20% of the control values. All three medicinal plants exhibit their traditionally known anti-obesity effects through cytostatic effects, modulation of proinflammatory and anti-inflammatory cytokine production levels, and reduction of adipocyte differentiation and fat accumulation.","PeriodicalId":9026,"journal":{"name":"Biointerface Research in Applied Chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49009728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Comparative Investigations on the Bioactivity of Surface Grain Refined Titanium and Surface Oxidized Titanium for Biomedical Implant Applications 表面晶粒细化钛与表面氧化钛在生物医学植入物中的生物活性比较研究

Biointerface Research in Applied Chemistry Pub Date : 2022-09-11 DOI: 10.33263/briac134.318

{"title":"Comparative Investigations on the Bioactivity of Surface Grain Refined Titanium and Surface Oxidized Titanium for Biomedical Implant Applications","authors":"","doi":"10.33263/briac134.318","DOIUrl":"https://doi.org/10.33263/briac134.318","url":null,"abstract":"Surface engineering of titanium (Ti) for medical implant applications is an active research area in the biomedical field across the globe. Improving the bioactivity of the Ti surface is crucial for implant applications where osseointegration is essentially required to enhance the healing rate. In the present work, shot peening followed by micro-arc oxidation (MAO) treatments were applied to pure Ti with an objective to investigate the role of surface grain refinement and the oxide layer on biomineralization ability to assess the bioactivity of the surface. After shot peening with steel balls, Ti substrates were subjected to MAO using sodium phosphate solution. Grain refinement was observed at the surface after the shot peening at a submicrometer levels ranging from 0.5 to 2 µm for a thickness of ~ 50µm. Ti sheets subjected to MAO exhibited a porous oxide layer on the surface. From the XRD analysis, the TiO2 layer was observed as a combination of anatase and rutile. Higher Ca/P-based apatite deposition on shot-peened Ti compared with MAO Ti was observed in the in vitro immersion studies. The results indicated increased bioactivity for grain refined Ti compared with MAO Ti. Hence, it is concluded that the microstructure influences the bioactivity of Ti implants compared with the oxide layer.","PeriodicalId":9026,"journal":{"name":"Biointerface Research in Applied Chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48677599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical, Biological, and Pharmacological Prospects of Caffeic Acid 咖啡酸的化学、生物学和药理学前景

Biointerface Research in Applied Chemistry Pub Date : 2022-09-11 DOI: 10.33263/briac134.324

{"title":"Chemical, Biological, and Pharmacological Prospects of Caffeic Acid","authors":"","doi":"10.33263/briac134.324","DOIUrl":"https://doi.org/10.33263/briac134.324","url":null,"abstract":"There are many proven beneficial pharmacological effects of polyphenols, and these compounds have been tremendously studied for their role in the human body. Daily intake of polyphenols has shown beneficial effects on the immune system. Polyphenols are the natural compounds present in plants as secondary metabolites. Caffeic acid (CA) is the major one among other compounds of hydroxycinnamic acid, which plays an important role as an antioxidant, anticancer, antidiabetic, antihypertensive, antimicrobial, hepatoprotective, antiviral, etc. It is found in most herbal plants. CA produces its pharmacological effect by altering the activity of various key enzymes. It reduces the blood glucose level by inhibiting enzymes α-amylase and α-glucosidase in type-2 diabetes. It shows anticancer and anti-inflammatory activity by inhibiting various transcript factors. The small part of the esterified form of CA is absorbed in the stomach, and the rest of the part is the breakdown in its free form by the microbial esterases in the colon. It enters intestinal cells via active transport mediated by MCT. The maximum plasma concentration is seen after one hour of food ingestion. Methylation, sulphation, and glucuronidation take place after absorption and are excreted primarily through urine. The purpose of this review is to enhance researchers' knowledge to conduct more studies to reveal and optimize CA's biological and pharmacological properties. Based on its pharmacological activity, this compound can be used as a natural safeguard to replace synthetic antibiotics and other synthetic medicine to reduce the medicinal cost and side effects.","PeriodicalId":9026,"journal":{"name":"Biointerface Research in Applied Chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44866227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2