ACS Biomaterials Science & Engineering最新文献

{"title":"Phage-Derived Depolymerase-Functionalized Coatings Prevent Staphylococcal Biofilm Formation","authors":"Steven De Soir,&nbsp;Cédric Vranckx,&nbsp;Agnieszka Latka,&nbsp;Yves Briers,&nbsp;Jeroen Wagemans,&nbsp;Rob Lavigne,&nbsp;Jean-Paul Pirnay,&nbsp;Daniel De Vos,&nbsp;Christine Dupont-Gillain,&nbsp;Françoise Van Bambeke","doi":"10.1021/acsbiomaterials.6c01098","DOIUrl":"10.1021/acsbiomaterials.6c01098","url":null,"abstract":"<p>Biofilm-associated infections caused by Staphylococci remain a major challenge in the context of biomedical implants due to their tolerance to antibiotics and resistance to host defenses. Here, we report the identification of a novel phage-derived depolymerase (DPO2) targeting staphylococcal biofilms and its integration into hyaluronic acid-based layer-by-layer (LbL) coatings. DPO2 was produced recombinantly and proved more effective preventing biofilm formation than destroying preformed <em>Staphylococcus epidermidis</em> biofilms. DPO2 antibiofilm activity was retained and even enhanced upon immobilization within DPO2/Hyaluronic Acid multilayered coatings. The resulting functionalized surfaces exhibited potent inhibition of biofilm formation by multiple <em>S. epidermidis</em> and <em>Staphylococcus aureus</em> strains from orthopedic infections, reaching nearly complete suppression under the tested conditions. This work demonstrates the potential of combining phage-derived enzymes with biomaterial surface engineering to develop efficient, non-antibiotic antibiofilm strategies. Such coatings represent a promising approach for preventing implant-associated infections.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"12 11","pages":"6471–6483"},"PeriodicalIF":6.0,"publicationDate":"2026-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149284243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional Three-Dimensional-Printed Bio-Ink from Waterborne Polyurethane and Natural Antioxidants for Advanced Wound Dressings","authors":"Hossein Baniasadi,&nbsp;Ubaid Tariq,&nbsp;Roozbeh Abidnejad,&nbsp;Prerna Singh,&nbsp;Jukka Niskanen,&nbsp;Jukka Sepäälä,&nbsp;Ashok Kumar","doi":"10.1021/acsbiomaterials.6c01170","DOIUrl":"10.1021/acsbiomaterials.6c01170","url":null,"abstract":"<p>The development of multifunctional wound dressings capable of simultaneously addressing oxidative stress, bacterial infection, and tissue regeneration remains a major challenge in advanced wound care. Here, we report a printable, bioactive 3D-printed dressing based on a synergistic bio-ink composed of waterborne polyurethane (WPU), pomegranate peel-derived antioxidant polyphenols (POM), and TEMPO-oxidized cellulose nanofibrils (TOCNF). POM was incorporated into the WPU matrix during in situ polymerization to introduce pomegranate peel-derived antioxidant and antibacterial functionality, while TOCNF served as a rheology modifier enabling extrusion-based direct ink writing. The resulting ink exhibited pronounced shear-thinning behavior, rapid viscosity recovery, and tunable yield stress, enabling successful, stable extrusion-based printing of multilayer architectures. Printed freeze-dried wound dressings exhibited highly interconnected porous structures (&gt;90% porosity), robust swelling capacity, structural integrity under physiological conditions, and compressive stability after hydration. The incorporation of POM enhanced antibacterial performance against both <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> and provided strong free-radical scavenging activity. In vitro studies using HaCaT keratinocytes confirmed excellent cytocompatibility, enhanced metabolic activity, and significant attenuation of H₂O₂-induced oxidative stress. Notably, this wound dressing reduced intracellular reactive oxygen species levels and improved cell survival under oxidative challenge. This work establishes a strategy for integrating natural antioxidant bioactives into printable polyurethane networks to produce structurally programmable, biologically active wound dressings. The combination of rheologically optimized printability, hierarchical porosity, and multifunctional bioactivity highlights the potential of this platform for advanced wound-dressing applications and other oxidative-stress-related tissue-repair strategies.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"12 11","pages":"6401–6416"},"PeriodicalIF":6.0,"publicationDate":"2026-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149284246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular Vesicles: Emerging Players in Tumor Progression, Diagnosis, and Therapeutic Applications","authors":"Lingyu Kong,&nbsp;Wanying Xu,&nbsp;Yue Niu,&nbsp;Nan Li,&nbsp;Xingye Fan,&nbsp;Xiaolin Wu,&nbsp;Jiaxuan Wu,&nbsp;Yueqiu Jiang,&nbsp;Yibo Gao,&nbsp;Yi Liu,&nbsp;Tao Yan,&nbsp;Shuang Ma","doi":"10.1021/acsbiomaterials.6c00978","DOIUrl":"10.1021/acsbiomaterials.6c00978","url":null,"abstract":"<p>Extracellular vesicles (EVs) are emerging as critical players in oncology and also exert vital regulatory effects on exercise-induced muscle injury repair, offering novel insights into tumor biology. Tumor-derived EVs facilitate intercellular communication by carrying bioactive molecules such as proteins and nucleic acids, which reshape the tumor microenvironment and enhance tumor invasion, metastasis, and drug resistance. Conversely, specific subsets of EVs, particularly those derived from dendritic cells or engineered to present tumor antigens, can elicit antitumor immune responses by activating cytotoxic T lymphocytes and facilitating antigen cross-presentation. Clinically, the high stability of EVs in body fluids, coupled with their intrinsic capacity to encapsulate and protect tumor-derived molecular cargo, positions them as robust noninvasive biomarkers for early cancer diagnosis and prognostic stratification, as well as indicators for evaluating muscle recovery status after exercise. Additionally, EVs function as drug delivery carriers, offering advantages like biocompatibility, low immunogenicity, and targeting capabilities. However, challenges remain in EVs isolation, characterization, and scalable production, with inconsistent techniques affecting purity, structure, and biological activity. Moreover, limited understanding of EVs mechanisms, such as their role in immune modulation and drug delivery efficiency, hinders broader clinical translation across oncology and musculoskeletal rehabilitation. Addressing these issues requires advances in standardization, scalable manufacturing, and extensive clinical trials to ensure its safety and efficacy. Despite these challenges, EVs hold immense promise as transformative tools in precision oncology and sports rehabilitation, paving the way for innovative diagnostics, therapies, and improving patient and athletic outcomes in the fight against cancer and exercise-related muscle damage.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"12 11","pages":"5915–5942"},"PeriodicalIF":6.0,"publicationDate":"2026-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149177921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formation of Coacervates from Charged Polypeptides for Cryopreservation","authors":"Zhenghua Zeng,&nbsp;Liang Yuan,&nbsp;Kongying Zhu,&nbsp;Lixia Ren,&nbsp;Xiaoyan Yuan","doi":"10.1021/acsbiomaterials.6c01215","DOIUrl":"10.1021/acsbiomaterials.6c01215","url":null,"abstract":"<p>Bioinspired antifreeze polypeptides can be used as macromolecular cryoprotectants, but the wide application of charged polypeptides remains a challenge for cell cryopreservation due to the possible membrane-destructive activity. In this work, positively charged poly(<span>l</span>-methionine) derivatives tethering threonine-mimetic methyl and hydroxyl groups (PMOH) are synthesized, which can form coacervates with anionic γ-poly(glutamic acid) (PGA) with or without trehalose via liquid–liquid phase separation. The coacervate consisting of PMOH and PGA each at 20 mg/mL with 0.2 M trehalose exhibits a strong ice recrystallization inhibition activity with a mean largest grain size of 22.2 ± 1.9% relative to PBS, attributable to the depressed diffusion of water molecules through the highly confined water and contribution of trehalose. The coacervation of PMOH/PGA could also promote ice nucleation owing to the charged nature of the polymers. Actually, the PMOH/PGA coacervates show acceptable cytocompatibility and cryopreservative effects on cell cryopreservation by reducing half of the dimethyl sulfoxide dosage. This work offers a valuable strategy for the utility of charged polypeptides in cryopreservation.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"12 11","pages":"6499–6510"},"PeriodicalIF":6.0,"publicationDate":"2026-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149100946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanozymes in Upper Respiratory Tract Infections: Catalytic Mechanisms, Current Evidence, and Emerging Theranostic Strategies","authors":"Jingjing Huang,&nbsp;Zhuyu Han,&nbsp;Yang Liu,&nbsp;Jiechao Yang,&nbsp;Jiafu Liu,&nbsp;Yiwen Tao,&nbsp;Fangyong Zhu,&nbsp;Zelda Ziyi Zhao","doi":"10.1021/acsbiomaterials.6c01534","DOIUrl":"10.1021/acsbiomaterials.6c01534","url":null,"abstract":"<section><div></div><p>Objective: This review evaluates nanozymes as catalytic biomaterials for upper respiratory tract infection (URTI)-related diagnosis, therapy, and prevention, with particular attention to differences in the maturity of evidence across these applications. The anatomically adjacent middle ear is also considered as a relevant model of biofilm-associated infection and barrier-limited local delivery. Methods: We reviewed studies involving respiratory pathogens, sinonasal disease, pharyngitis, intranasal vaccination, and otitis media. Evidence from other disease models was included only when it addressed a specific materials problem relevant to the upper airway, such as catalytic regulation, biofilm disruption, mucus transport, or local retention. Results: Diagnostic applications currently have the strongest evidence base, particularly for catalytic signal amplification in respiratory pathogen assays. In contrast, therapeutic evidence remains sparse and predominantly preclinical, with direct treatment studies in URTIs especially limited. Preventive applications are less extensively studied and are represented mainly by proof-of-concept intranasal influenza vaccination. Across these applications, performance depends on catalytic activity in protein- and mucus-rich media, biofilm penetration, control of pro-oxidant and antioxidant effects, and delivery to the intended anatomical site. Evidence from other anatomical sites provides useful design principles but should not be interpreted as evidence of efficacy in URTIs. Conclusion: Nanozymes should currently be regarded as engineerable catalytic biomaterials rather than clinically established anti-infective platforms for URTIs. Translation will require reproducible activity in airway-relevant environments, effective site-specific delivery, appropriate safety margins, and validation in disease-relevant models.</p></section>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"12 11","pages":"6054–6066"},"PeriodicalIF":6.0,"publicationDate":"2026-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149134046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-Assembled Platelet-Derived Growth Factor-Mimetic Peptide@BG Hydrogel Promotes Wound Healing by Regulating Mitochondrial Function through the RhoA/Rock1/Drp1 Signaling Axis","authors":"Yun Xu,&nbsp;Fei Hu,&nbsp;Chenyu Ma,&nbsp;Xuebo Wei,&nbsp;Yingjian Zhang,&nbsp;Xiaobin Cai,&nbsp;Yinan Lan","doi":"10.1021/acsbiomaterials.6c01074","DOIUrl":"10.1021/acsbiomaterials.6c01074","url":null,"abstract":"<p>Skin wound healing involves four overlapping phases, with angiogenesis and mitochondrial functional recovery being critical for effective repair. In this study, a platelet-derived growth factor (PDGF)-mimetic peptide hydrogel was constructed and combined with bioactive glass to form a PDGF-mimetic peptide@BG composite system, aiming to synergistically promote wound healing. The structural stability and morphology of the PDGF-mimetic peptide@BG were characterized using electron microscopy and spectroscopic techniques. Biocompatibility was assessed through in vitro cell experiments. A full-thickness skin defect model in mice was established, and wound healing, angiogenesis, and mitochondrial functional recovery were evaluated using histological staining, Western blotting, and immunofluorescence. The involvement of the RhoA/Rock1/Drp1 signaling pathway in mitochondrial function improvement was investigated. The PDGF-mimetic peptide@BG was successfully prepared with stable structure. In vitro experiments demonstrated good biocompatibility and promotion of endothelial cell proliferation and migration. In vivo results confirmed that PDGF-mimetic peptide@BG accelerated wound healing in mice, enhanced granulation tissue formation and collagen deposition, and increased local blood flow and angiogenesis. Furthermore, the composite restored mitochondrial function, as evidenced by reduced ROS levels, elevated ATP and SOD activity, improved mitochondrial morphology, and downregulation of mitochondrial fission-related proteins. Mechanistic studies suggested that PDGF-mimetic peptide@BG may exert its reparative effects at least in part through inhibition of the RhoA/Rock1/Drp1 signaling pathway. This study suggests that the PDGF-mimetic peptide@BG significantly promotes tissue regeneration in a normal murine wound model by increasing wound blood flow, enhancing vascularization, accelerating mitochondrial repair, and improving its structure and function. These findings provide a preliminary foundation for further evaluation in clinically relevant impaired-healing models.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"12 11","pages":"6376–6387"},"PeriodicalIF":6.0,"publicationDate":"2026-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149206248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Modular Glycoengineered Exosome Platform for Targeted Delivery via Chemoenzymatic Engineering of Surface Sialic Acids","authors":"Ziwei Wu,&nbsp;Ronglian Xu,&nbsp;Luncai Chen,&nbsp;Ling Zhang,&nbsp;Shihan Wang,&nbsp;Xiaorong Fan,&nbsp;Ziang Wang,&nbsp;Xinru Zhang,&nbsp;Zhifang Zhou,&nbsp;Haofei Hong,&nbsp;Zhimeng Wu,&nbsp;Jie Shi","doi":"10.1021/acsbiomaterials.6c01221","DOIUrl":"10.1021/acsbiomaterials.6c01221","url":null,"abstract":"<p>Bovine milk-derived exosomes (mExos) are scalable, biocompatible nanocarriers well-suited for the cross-barrier transport and protection of labile nucleic acid therapeutics. However, achieving precise active targeting for cargo delivery remains challenging, as conventional surface functionalization methods often lack site-specificity, unintentionally altering native physicochemical properties or obscuring essential membrane interactions. Herein, we report a modular glycoengineering strategy that leverages the dense sialic acid coating on mExos to introduce versatile anchoring sites for targeting ligand modification via sequential chemoenzymatic remodeling. Specifically, the native terminal sialic acids on the mExo surface were enzymatically removed and replaced with azide-modified sialic acid analogs, which was achieved efficiently through an integrated multienzyme reaction. In principle, the resulting platform, mExo-N₃, enables the glycan-selective conjugation of any dibenzocyclooctyne (DBCO)-modified targeting ligands for targeted delivery, avoiding the limitations of random modifications. To validate this platform, we conjugated mExo-N₃ with an anti-EGFR nanobody (7D12) and encapsulated a microRNA-21 inhibitor (miR-21i). The resulting mExo-7D12@miR-21i construct maintained colloidal stability and exhibited specific internalization by EGFR-positive cancer cells. Intracellular delivery of miR-21i restored the expression of tumor suppressors PTEN and PDCD4, inducing apoptosis and suppressing proliferation. Furthermore, in an A431 xenograft model, the targeted system achieved 58.3% tumor growth inhibition <em>in vivo</em> without evident systemic toxicity. By converting native glycans into functional anchoring sites, this approach provides a translatable framework for customizing exosomal delivery vectors into targeted therapeutics.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"12 11","pages":"6245–6260"},"PeriodicalIF":6.0,"publicationDate":"2026-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149222904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quorum-Sensing-Mediated Extracellular Electron Transfer Enables Hydrogel Morphogenesis","authors":"Ismar E. Miniel Mahfoud,&nbsp;Vidhika S. Damani,&nbsp;Gina Partipilo,&nbsp;Andy Y. Liu,&nbsp;Benjamin K. Keitz","doi":"10.1021/acsbiomaterials.6c01127","DOIUrl":"10.1021/acsbiomaterials.6c01127","url":null,"abstract":"<p>Engineered living materials seek to capture the sensitivity, responsiveness, and programmable characteristics of biological systems. One emergent property of living systems is genetically driven spatial patterning, which controls cell differentiation and the development of complex multicellular organisms. Inspired by this capability, we use bacteria to spatially control material assembly. In our system, extracellular electron transfer (EET) flux from <em>Shewanella oneidensis</em> drives hydrogel synthesis via copper-catalyzed radical polymerization. We first construct a recombinant quorum-sensing system in <em>S. oneidensis</em> that allows for cell-cell communication between “sender” and “receiver” cells through an autoinducer. We then examine controlled gene expression and EET-driven chemical transformation in various synthetic consortia. Via diffusion through agarose, we examine 2D patterns of gene expression relative to localized sender cell populations and demonstrate controlled hydrogel crosslinking in predictable patterns. Finally, we apply computational methods and NOT logic in “receiver” cells toward more complex patterns of gene expression. Our results highlight the potential of bacteria to program material systems with life-like properties, including self-assembly, environmental responsiveness, and patterned differentiation.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"12 11","pages":"6092–6106"},"PeriodicalIF":6.0,"publicationDate":"2026-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149226746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidized Pectin-Amine-Modified Chitosan/PEDOT with Decellularized Human Spinal Cord-Based Self-Healing and Electroconductive Hydrogel for Potential Spinal Regeneration Applications","authors":"Gopal Agarwal,&nbsp;Samantha Shumard,&nbsp;Matthew Callaway,&nbsp;John G. Hardy,&nbsp;Christine E. Schmidt","doi":"10.1021/acsbiomaterials.6c00957","DOIUrl":"10.1021/acsbiomaterials.6c00957","url":null,"abstract":"<p>Decellularized extracellular matrix (dECM)-based biomaterials have emerged as promising therapeutic platforms for spinal cord injury (SCI) repair due to their ability to provide regenerative biochemical cues. In this study, we developed an injectable, electroconductive, and self-healing hydrogel for potential application in SCI repair. Injectability enables minimally invasive delivery, electroconductivity facilitates electrical communication that may promote neural regeneration, and self-healing properties allow restoration of the hydrogel network following shear stress during injection. The self-healing hydrogel was formulated using Schiff-base chemistry between aldehyde-functionalized pectin (PEC-ALD) and amine-functionalized chitosan (CS). Poly(3,4-ethylenedioxythiophene) (PEDOT) derivatives were incorporated to enhance electrical conductivity and support neuronal differentiation and axonal outgrowth. In addition, decellularized human spinal cord (dSC), prepared using a modified Hudson protocol, was incorporated to provide tissue-specific pro-regenerative cues. The resulting PEC-ALD-CS-PEDOT-dSC hydrogels exhibited dynamic self-healing behavior characteristic of native spinal cord tissue. Under high oscillatory strain (1000%), the hydrogel network structure temporarily dissociated, while recovery of the crosslinked network occurred upon reduction to low strain (1%). The hydrogels demonstrated a storage modulus of approximately 600 Pa, consistent with the mechanical properties favorable for neuronal growth. Incorporation of PEDOT derivatives resulted in an electrical conductivity of approximately 5.6 mS/cm. Schiff base-mediated crosslinking enabled rapid gelation within 20 min at 37 °C. Furthermore, the hydrogels exhibited shear-thinning behavior, supporting their suitability for injectable delivery. Biological evaluation demonstrated immunomodulatory potential, as RAW 264.7 macrophages encapsulated within the hydrogel exhibited greater CD206 expression relative to CD86 expression, even under lipopolysaccharide-induced inflammatory conditions. Additionally, the hydrogels supported proliferation of human SH-SY5Y neuroblastoma cells, indicating cytocompatibility and potential to support neural regeneration. Overall, this study presents a multifunctional self-healing and electroconductive hydrogel platform incorporating decellularized spinal cord matrix for potential minimally invasive treatment of SCI.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"12 11","pages":"6333–6349"},"PeriodicalIF":6.0,"publicationDate":"2026-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149259619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances and Challenges of Sensing in Water Using CRISPR-Cas Technology","authors":"Moein Safarkhani,&nbsp;Bahareh Farasati Far,&nbsp;Su-Hyeon Kim,&nbsp;Pooyan Makvandi,&nbsp;Mi-Kyung Park,&nbsp;YunSuk Huh,&nbsp;Navid Rabiee","doi":"10.1021/acsbiomaterials.3c01689","DOIUrl":"10.1021/acsbiomaterials.3c01689","url":null,"abstract":"<p>The groundbreaking gene-editing mechanism, CRISPR (ClusteredRegularlyInterspaced Short Palindromic Repeats), paired with the protein Cas9,has significantly advanced the realms of biology, medicine, and agriculture.Through its precision in modifying genetic sequences, CRISPR holdsthe potential to alter the trajectory of genetic disorders and accelerateadvancements in agriculture. While its therapeutic potential is profound,the technology also invites ethical debates centered on responsibleuse and equity in access. Parallelly, in the environmental monitoringsphere and sensing in water, especially biosensors have been instrumentalin evaluating natural water sources’ quality. These biosensors,integrating biological components with detection techniques, havethe potential to revolutionize healthcare by providing rapid and minimallyinvasive diagnostic methods. However, the design and application ofthese sensors bring forth challenges, especially in ensuring sensitivity,selectivity, and ethical data handling. This article delves into theprospective use of CRISPR-Cas technology for sensing in water, exploringits capabilities in detecting diverse biomarkers, hazardous substances,and varied reactions in water and wastewater systems.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"12 11","pages":"5723–5738"},"PeriodicalIF":6.0,"publicationDate":"2026-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140565147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}