磁性纳米颗粒和单宁酸修饰的静电纺PCL垫具有抗菌和可能的抗骨组织工程和癌症治疗骨肉瘤活性。

IF 5.4 2区 医学 Q2 MATERIALS SCIENCE, BIOMATERIALS
Anna Hlukhaniuk, Małgorzata Świętek, Vitalii Patsula, Olga Janoušková, Antonín Brož, Marina Malić, Anna Kołodziej, Aleksandra Wesełucha-Birczyńska, Jiří Hodan, Miroslav Slouf, Waldemar Tokarz, Beata Zasońska, Lukáš Bystrianský, Milan Gryndler, Lucie Bačáková, Daniel Horák
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引用次数: 0

摘要

使用同时对成骨细胞产生积极影响并对癌变生长产生负面影响的药物修饰支架,是肿瘤切除后骨组织缺陷患者的一种有希望的解决方案。这种材料不仅可以促进组织再生,还可以限制癌症复发的风险。在我们的研究中,我们开发了一种新型的双功能支架,该支架含有磁性纳米颗粒,嫁接了PCL (MNP@PCL)和单宁酸(TA),可能用于支持正常骨细胞和抑制骨肉瘤细胞。首先,通过ε-己内酯开环聚合从表面接枝聚(ε-己内酯)(PCL),对MNPs进行了合成后的表面修饰,使其在聚合物基体内均匀分布。然后采用静电纺丝法制备一定量MNPs (2 wt %)和增加TA含量(0、1、5、10 wt %)的纤维垫。MNP@PCL和TA都降低了聚合物的结晶度。MNPs和TA之间的相互作用显著影响了毡的形态、热性能和初始水解性能。最密集的TA释放主要发生在孵育前6小时,与mfPCL@TA-5相比,mfPCL@TA-10的TA释放量高出3.5倍(每mg mat约0.02 mg TA)。此外,含ta的磁垫抑制骨肉瘤细胞的代谢活性。与纯PCL材料相比,他们还展示了增强的抗菌性能,可以对抗通常伴随骨科并发症的细菌,将革兰氏阳性细菌的数量减少90%以上。这证明了这些材料在将癌症治疗与骨组织工程相结合方面的巨大潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Electrospun PCL Mats Modified with Magnetic Nanoparticles and Tannic Acid with Antibacterial and Possible Antiosteosarcoma Activity for Bone Tissue Engineering and Cancer Treatment.

Modifying scaffolds with agents that at the same time positively influence osteogenic cells and have a negative impact on cancerous growth, is a promising solution for patients with bone tissue defects following tumor excision. Such materials may not only boost tissue regeneration but also limit the risk of cancer reoccurrence. In our study, we developed novel bifunctional scaffolds containing magnetic nanoparticles grafted with PCL (MNP@PCL) and tannic acid (TA), which may be directed to support normal bone cells and suppress osteosarcoma cells. First, MNPs were postsynthetically surface-modified, by grafting poly(ε-caprolactone) (PCL) from the surface via ring opening polymerization of ε-caprolactone, to provide their uniform distribution within the polymer matrix. Then, fiber mats containing a fixed amount of MNPs (2 wt %) and increasing content of TA (0, 1, 5, and 10 wt %) were prepared by electrospinning method. Both MNP@PCL and TA decreased polymer crystallinity. The interaction between the MNPs and TA significantly influenced the mat morphology, thermal properties, and initial hydrolytic performance. The most intensive TA release was observed mainly within first 6 h of incubation, and it was 3.5-fold higher (ca. 0.02 mg of TA/per mg of mat) for mfPCL@TA-10 compared to mfPCL@TA-5. Moreover, TA-containing magnetic mats suppressed the metabolic activity of osteosarcoma cells. They also demonstrated enhanced antimicrobial properties against the bacteria typically accompanying orthopedic complications, reducing the population of Gram-positive bacteria by more than 90% compared to the neat PCL mat. This proves the high potential of these materials for combining cancer treatment with bone tissue engineering.

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来源期刊
ACS Biomaterials Science & Engineering
ACS Biomaterials Science & Engineering Materials Science-Biomaterials
CiteScore
10.30
自引率
3.40%
发文量
413
期刊介绍: ACS Biomaterials Science & Engineering is the leading journal in the field of biomaterials, serving as an international forum for publishing cutting-edge research and innovative ideas on a broad range of topics: Applications and Health – implantable tissues and devices, prosthesis, health risks, toxicology Bio-interactions and Bio-compatibility – material-biology interactions, chemical/morphological/structural communication, mechanobiology, signaling and biological responses, immuno-engineering, calcification, coatings, corrosion and degradation of biomaterials and devices, biophysical regulation of cell functions Characterization, Synthesis, and Modification – new biomaterials, bioinspired and biomimetic approaches to biomaterials, exploiting structural hierarchy and architectural control, combinatorial strategies for biomaterials discovery, genetic biomaterials design, synthetic biology, new composite systems, bionics, polymer synthesis Controlled Release and Delivery Systems – biomaterial-based drug and gene delivery, bio-responsive delivery of regulatory molecules, pharmaceutical engineering Healthcare Advances – clinical translation, regulatory issues, patient safety, emerging trends Imaging and Diagnostics – imaging agents and probes, theranostics, biosensors, monitoring Manufacturing and Technology – 3D printing, inks, organ-on-a-chip, bioreactor/perfusion systems, microdevices, BioMEMS, optics and electronics interfaces with biomaterials, systems integration Modeling and Informatics Tools – scaling methods to guide biomaterial design, predictive algorithms for structure-function, biomechanics, integrating bioinformatics with biomaterials discovery, metabolomics in the context of biomaterials Tissue Engineering and Regenerative Medicine – basic and applied studies, cell therapies, scaffolds, vascularization, bioartificial organs, transplantation and functionality, cellular agriculture
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