Biofabrication最新文献_第5页

Standalone single- and bi-layered human skin 3D models supported by recombinant silk feature native spatial organization. 由重组丝支持的独立单层和双层人体皮肤三维模型具有原生空间组织特征。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-11-05 DOI: 10.1088/1758-5090/ad8b72

Savvini Gkouma, Nayanika Bhalla, Solène Frapard, Alexander Jönsson, Hakan Gürbüz, Asli Aybike Dogan, Stefania Giacomello, Martin Duvfa, Patrik L Ståhl, Mona Widhe, My Hedhammar

{"title":"Standalone single- and bi-layered human skin 3D models supported by recombinant silk feature native spatial organization.","authors":"Savvini Gkouma, Nayanika Bhalla, Solène Frapard, Alexander Jönsson, Hakan Gürbüz, Asli Aybike Dogan, Stefania Giacomello, Martin Duvfa, Patrik L Ståhl, Mona Widhe, My Hedhammar","doi":"10.1088/1758-5090/ad8b72","DOIUrl":"10.1088/1758-5090/ad8b72","url":null,"abstract":"Physiologically relevant human skin models that include key skin cell types can be used forin vitrodrug testing, skin pathology studies, or clinical applications such as skin grafts. However, there is still no golden standard for such a model. We investigated the potential of a recombinant functionalized spider silk protein, FN-silk, for the construction of a dermal, an epidermal, and a bilayered skin equivalent (BSE). Specifically, two formats of FN-silk (i.e. 3D network and nanomembrane) were evaluated. The 3D network was used as an elastic ECM-like support for the dermis, and the thin, permeable nanomembrane was used as a basement membrane to support the epidermal epithelium. Immunofluorescence microscopy and spatially resolved transcriptomics analysis demonstrated the secretion of key ECM components and the formation of microvascular-like structures. Furthermore, the epidermal layer exhibited clear stratification and the formation of a cornified layer, resulting in a tight physiologic epithelial barrier. Our findings indicate that the presented FN-silk-based skin models can be proposed as physiologically relevant standalone epidermal or dermal models, as well as a combined BSE.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142494588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AI for biofabrication. 用于生物制造的人工智能

IF 8.2 2区医学

Biofabrication Pub Date : 2024-11-04 DOI: 10.1088/1758-5090/ad8966

Chang Zhou, Changru Liu, Zhendong Liao, Yuan Pang, Wei Sun

{"title":"AI for biofabrication.","authors":"Chang Zhou, Changru Liu, Zhendong Liao, Yuan Pang, Wei Sun","doi":"10.1088/1758-5090/ad8966","DOIUrl":"10.1088/1758-5090/ad8966","url":null,"abstract":"Biofabrication is an advanced technology that holds great promise for constructing highly biomimeticin vitrothree-dimensional human organs. Such technology would help address the issues of immune rejection and organ donor shortage in organ transplantation, aiding doctors in formulating personalized treatments for clinical patients and replacing animal experiments. Biofabrication typically involves the interdisciplinary application of biology, materials science, mechanical engineering, and medicine to generate large amounts of data and correlations that require processing and analysis. Artificial intelligence (AI), with its excellent capabilities in big data processing and analysis, can play a crucial role in handling and processing interdisciplinary data and relationships and in better integrating and applying them in biofabrication. In recent years, the development of the semiconductor and integrated circuit industries has propelled the rapid advancement of computer processing power. An AI program can learn and iterate multiple times within a short period, thereby gaining strong automation capabilities for a specific research content or issue. To date, numerous AI programs have been applied to various processes around biofabrication, such as extracting biological information, designing and optimizing structures, intelligent cell sorting, optimizing biomaterials and processes, real-time monitoring and evaluation of models, accelerating the transformation and development of these technologies, and even changing traditional research patterns. This article reviews and summarizes the significant changes and advancements brought about by AI in biofabrication, and discusses its future application value and direction.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanical memory based biofabrication of hierarchical elastic cardiac tissue. 基于机械记忆的分层弹性心脏组织生物制造。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-11-04 DOI: 10.1088/1758-5090/ad89fd

Zhitong Li, Panna Kovács, Alice Le Friec, Bjarke Nørrehvedde Jensen, Jens Vinge Nygaard, Menglin Chen

引用次数: 0

Fabrication of hierarchically porous trabecular bone replicas via 3D printing with high internal phase emulsions (HIPEs). 使用高内相乳液（HIPE）通过三维打印技术制造分层多孔骨小梁复制品。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-11-04 DOI: 10.1088/1758-5090/ad8b70

Nihan Sengokmen-Ozsoz, Mina Aleemardani, Marco Palanca, Alice Hann, Gwendolen C Reilly, Enrico Dall'Ara, Frederik Claeyssens

{"title":"Fabrication of hierarchically porous trabecular bone replicas via 3D printing with high internal phase emulsions (HIPEs).","authors":"Nihan Sengokmen-Ozsoz, Mina Aleemardani, Marco Palanca, Alice Hann, Gwendolen C Reilly, Enrico Dall'Ara, Frederik Claeyssens","doi":"10.1088/1758-5090/ad8b70","DOIUrl":"10.1088/1758-5090/ad8b70","url":null,"abstract":"Combining emulsion templating with additive manufacturing enables the production of inherently porous scaffolds with multiscale porosity. This approach incorporates interconnected porous materials, providing a structure that supports cell ingrowth. However, 3D printing hierarchical porous structures that combine semi-micropores and micropores remains a challenging task. Previous studies have demonstrated that using a carefully adjusted combination of light absorbers and photoinitiators in the resin can produce open surface porosity, sponge-like internal structures, and a printing resolution of about 150µm. In this study, we explored how varying concentrations of tartrazine (0, 0.02, 0.04, and 0.08 wt%) as a light absorber affect the porous structure of acrylate-based polymerized medium internal phase emulsions fabricated via vat photopolymerization. Given the importance of a porous and interconnected structure for tissue engineering and regenerative medicine, we tested cell behavior on these 3D-printed disk samples using MG-63 cells, examining metabolic activity, adhesion, and morphology. The 0.08 wt% tartrazine-containing 3D-printed sample (008 T) demonstrated the best cell proliferation and adhesion. To show that this high internal phase emulsion (HIPE) resin can be used to create complex structures for biomedical applications, we 3D-printed trabecular bone structures based on microCT imaging. These structures were further evaluated for cell behavior and migration, followed by microCT analysis after 60 days of cell culture. This research demonstrates that HIPEs can be used as a resin to print trabecular bone mimics using additive manufacturing, which could be further developed for lab-on-a-chip models of healthy and diseased bone.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142494585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scaffold-based tissue engineering strategies for urethral repair and reconstruction. 基于支架的尿道修复和重建组织工程策略。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-11-01 DOI: 10.1088/1758-5090/ad8965

Yangwang Jin, Ming Yang, Weixin Zhao, Meng Liu, Wenzhuo Fang, Yuhui Wang, Guo Gao, Ying Wang, Qiang Fu

引用次数: 0

Vascular smooth muscle cells can be circumferentially aligned inside a channel using tunable gelatin microribbons. 利用可调明胶微带，血管平滑肌细胞可在通道内周向排列。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-10-30 DOI: 10.1088/1758-5090/ad88a7

Yusuf Mastoor, Mahsa Karimi, Michael Sun, Fereshteh Ahadi, Pattie Mathieu, Mingyue Fan, Lin Han, Li-Hsin Han, Alisa Morss Clyne

{"title":"Vascular smooth muscle cells can be circumferentially aligned inside a channel using tunable gelatin microribbons.","authors":"Yusuf Mastoor, Mahsa Karimi, Michael Sun, Fereshteh Ahadi, Pattie Mathieu, Mingyue Fan, Lin Han, Li-Hsin Han, Alisa Morss Clyne","doi":"10.1088/1758-5090/ad88a7","DOIUrl":"10.1088/1758-5090/ad88a7","url":null,"abstract":"The gold standard to measure arterial health is vasodilation in response to nitric oxide. Vasodilation is generally measured via pressure myography of arteries isolated from animal models. However, animal arteries can be difficult to obtain and may have limited relevance to human physiology. It is, therefore, critical to engineer human cell-based arterial models capable of contraction. Vascular smooth muscle cells (SMCs) must be circumferentially aligned around the vessel lumen to contract the vessel, which is challenging to achieve in a soft blood vessel model. In this study, we used gelatin microribbons to circumferentially align SMCs inside a hydrogel channel. To accomplish this, we created tunable gelatin microribbons of varying stiffnesses and thicknesses and assessed how SMCs aligned along them. We then wrapped soft, thick microribbons around a needle and encapsulated them in a gelatin methacryloyl hydrogel, forming a microribbon-lined channel. Finally, we seeded SMCs inside the channel and showed that they adhered best to fibronectin and circumferentially aligned in response to the microribbons. Together, these data show that tunable gelatin microribbons can be used to circumferentially align SMCs inside a channel. This technique can be used to create a human artery-on-a-chip to assess vasodilation via pressure myography, as well as to align other cell types for 3Din vitromodels.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11583946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D bioprinting of an intervertebral disc tissue analogue with a highly aligned annulus fibrosus via suspended layer additive manufacture. 通过悬浮层快速成型技术三维生物打印具有高度排列的纤维环的椎间盘组织模拟物。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-10-24 DOI: 10.1088/1758-5090/ad8379

S R Moxon, Z McMurran, M J Kibble, M Domingos, J E Gough, S M Richardson

{"title":"3D bioprinting of an intervertebral disc tissue analogue with a highly aligned annulus fibrosus via suspended layer additive manufacture.","authors":"S R Moxon, Z McMurran, M J Kibble, M Domingos, J E Gough, S M Richardson","doi":"10.1088/1758-5090/ad8379","DOIUrl":"10.1088/1758-5090/ad8379","url":null,"abstract":"Intervertebral disc (IVD) function is achieved through integration of its two component regions: the nucleus pulposus (NP) and the annulus fibrosus (AF). The NP is soft (0.3-5 kPa), gelatinous and populated by spherical NP cells in a polysaccharide-rich extracellular matrix (ECM). The AF is much stiffer (∼100 kPa) and contains layers of elongated AF cells in an aligned, fibrous ECM. Degeneration of the disc is a common problem with age being a major risk factor. Progression of IVD degeneration leads to chronic pain and can result in permanent disability. The development of therapeutic solutions for IVD degeneration is impaired by a lack ofin vitromodels of the disc that are capable of replicating the fundamental structure and biology of the tissue. This study aims to investigate if a newly developed suspended hydrogel bioprinting system (termed SLAM) could be employed to fabricate IVD analogues with integrated structural and compositional features similar to native tissue. Bioprinted IVD analogues were fabricated to recapitulate structural, morphological and biological components present in the native tissue. The constructs replicated key structural components of native tissue with the presence of a central, polysaccharide-rich NP surrounded by organised, aligned collagen fibres in the AF. Cell tracking, actin and matrix staining demonstrated that embedded NP and AF cells exhibited morphologies and phenotypes analogous to what is observedin vivowith elongated, aligned AF cells and spherical NP cells that deposited HA into the surrounding environment. Critically, it was also observed that the NP and AF regions contained a defined cellular and material interface and segregated regions of the two cell types, thus mimicking the highly regulated structure of the IVD.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11499629/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142375046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Calcium phosphate complex of recombinant human thrombomodulin promote bone formation in interbody fusion. 重组人血栓调节蛋白磷酸钙复合物可促进椎间融合术中的骨形成。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-10-24 DOI: 10.1088/1758-5090/ad8035

Cheng-Li Lin, Yu-Wei Chen, Cheng-Hsiang Kuo, Ting-Yuan Tu, Hua-Lin Wu, Jui-Chen Tsai, Yan-Jye Shyong

{"title":"Calcium phosphate complex of recombinant human thrombomodulin promote bone formation in interbody fusion.","authors":"Cheng-Li Lin, Yu-Wei Chen, Cheng-Hsiang Kuo, Ting-Yuan Tu, Hua-Lin Wu, Jui-Chen Tsai, Yan-Jye Shyong","doi":"10.1088/1758-5090/ad8035","DOIUrl":"10.1088/1758-5090/ad8035","url":null,"abstract":"Interbody fusion is an orthopedic surgical procedure to connect two adjacent vertebrae in patients suffering from spinal disc disease. The combination of synthetic bone grafts with protein-based drugs is an intriguing approach to stimulate interbody bone growth, specifically in patients exhibiting restricted bone progression. Recombinant human thrombomodulin (rhTM), a novel protein drug characterized by its superior stability and potency, shows promise in enhancing bone formation. A composite bone graft, termed CaP-rhTM, has been synthesized, combining calcium phosphate (CaP) microparticles as a delivery vehicle for rhTM to facilitate interbody fusion.In vitrostudies have demonstrated that rhTM significantly promotes the proliferation and maturation of preosteoblasts at nanogram dosage, while exerting minimal impact on osteosarcoma cell growth. The expression levels of mature osteoblast markers, including osteocalcin, osteopontin, alkaline phosphatase, and calcium deposition were also enhanced by rhTM. In rat caudal disc model of interbody fusion, CaP-rhTM with 800 ng of drug dosage was implanted along with a polylactic acid cage, to ensure structural stability within the intervertebral space. Microcomputed tomography analyses revealed that from 8 to 24 weeks, CaP-rhTM substantially improves both bone volume and trabecular architecture, in addition to the textural integrity of bony endplate surfaces. Histological examination confirmed the formation of a continuous bone bridge connecting adjacent vertebrae. Furthermore, biomechanical assessment via three-point bending tests indicated an improved bone quality of the fused disc. This study has demostrated that rhTM exhibits considerable potential in promoting osteogenesis. The use of CaP-rhTM has also shown significant improvements in promoting interbody fusion.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142341030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacy 3D printing. 药房 3D 打印。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-10-24 DOI: 10.1088/1758-5090/ad837a

Jessica T Y Cheng, Edwin C K Tan, Lifeng Kang

{"title":"Pharmacy 3D printing.","authors":"Jessica T Y Cheng, Edwin C K Tan, Lifeng Kang","doi":"10.1088/1758-5090/ad837a","DOIUrl":"10.1088/1758-5090/ad837a","url":null,"abstract":"A significant limitation of the 'one size fits all' medication approach is the lack of consideration for special population groups. 3D printing technology has revolutionised the landscape of pharmaceuticals and pharmacy practice, playing an integral role in enabling on-demand production of customised medication. Compared to traditional pharmaceutical processes, 3D printing has major advantages in producing tailored dosage forms with unique drug release mechanisms. Moreover, this technology has enabled the combination of multiple drugs in a single formulation addressing key issues of medication burden. Development of 3D printing in pharmacy applications and large-scale pharmaceutical manufacturing has substantially increased in recent years. This review focuses on the emergence of extrusion-based 3D printing, particularly semi solid extrusion, fused deposition modelling and direct powder extrusion, which are currently the most commonly studied for pharmacy practice. The concept of each technique is summarised, with examples of current and potential applications. Next, recent advancements in the 3D printer market and pharmacist perceptions are discussed. Finally, the benefits, challenges and prospects of pharmacy 3D printing technology are highlighted, emphasising its significance in changing the future of this field.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142375047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On-chip human lymph node stromal network for evaluating dendritic cell and T-cell trafficking. 用于评估树突状细胞和 T 细胞迁移的片上人体淋巴结基质网络。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-10-24 DOI: 10.1088/1758-5090/ad80ce

Brian J Kwee, Mona Mansouri, Adovi Akue, Kyung E Sung

{"title":"On-chip human lymph node stromal network for evaluating dendritic cell and T-cell trafficking.","authors":"Brian J Kwee, Mona Mansouri, Adovi Akue, Kyung E Sung","doi":"10.1088/1758-5090/ad80ce","DOIUrl":"10.1088/1758-5090/ad80ce","url":null,"abstract":"The lymph node paracortex, also known as the T-cell zone, consists of a network of fibroblastic reticular cells (FRCs) that secrete chemokines to induce T-cell and dendritic cell (DC) trafficking into the paracortex. To model the lymph node paracortex, we utilize multi-channel microfluidic devices to engineer a 3D lymph node stromal network from human cultured FRCs embedded in a collagen I-fibrin hydrogel. In the hydrogel, the FRCs self-assemble into an interconnected network, secrete the extracellular matrix proteins entactin, collagen IV, and fibronectin, as well as express an array of immune cell trafficking chemokines. Although the engineered FRC network did not secrete characteristic CCR7-ligand chemokines (i.e. CCL19 and CCL21), human primary TNF-αmatured monocyte-derived DCs, CD45RA+T-cells, and CD45RA-T-cells migrate toward the lymph node stromal network to a greater extent than toward a blank hydrogel. Furthermore, the FRCs co-recruit DCs and antigen-specific T-cells into the lymph node stromal network. This engineered lymph node stromal network may help evaluate how human DCs and T-cells migrate into the lymph node paracortex via CCR7-independent mechanisms.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142341031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0