Biofabrication最新文献

{"title":"CORRIGENDUM: Curcumin nanoparticles combined with 3D printed bionic tumor models for breast cancer treatment (2023<i>Biofabrication</i> 15 014105).","authors":"Ya Su, Xueyan Hu, Yue Kang, Cheng Zhang, Yuen Yee Cheng, Zeren Jiao, Yi Nie, Kedong Song","doi":"10.1088/1758-5090/ae98bb","DOIUrl":"https://doi.org/10.1088/1758-5090/ae98bb","url":null,"abstract":"","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":"18 3","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148807893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-step coaxial bioprinting of aligned and vascularized 3D skeletal muscle constructs.","authors":"Yeonjin Hong, Hyeongu Lim, Hyungseok Lee","doi":"10.1088/1758-5090/ae9597","DOIUrl":"10.1088/1758-5090/ae9597","url":null,"abstract":"<p><p>Skeletal muscle constitutes approximately 40%-50% of total body weight and plays a vital role in human physiology; however, its regenerative capacity is insufficient in cases of volumetric muscle loss, and effective therapeutic options remain limited. To address this challenge, tissue engineering approaches have focused on constructing functional muscle grafts, with increasing attention on vascularized muscle tissues due to their enhanced integration, regeneration, and reduced fibrosis after transplantation. Nevertheless, most existing strategies rely on complex, multi-step fabrication processes requiring external scaffolds or stimulation systems, which limit practical applicability. In this study, we propose a one-step coaxial bioprinting strategy that enables the simultaneous fabrication of aligned muscle and vascular structures within a single construct. By optimizing the bioink formulation, both structural integrity and cellular functionality were achieved. Shear stress generated during coaxial printing induced myoblast alignment, promoting myogenic differentiation, while precise compartmentalization ensured effective domain separation. Furthermore, dynamic culture conditions enhanced endothelial cell maturation and vascular functionality. This scaffold-free and mechanical-stimulus-free vascularized muscle construct provides a simplified yet functional platform with strong potential for muscle disease modeling, drug screening, and regenerative therapies. The proposed fabrication strategy represents a meaningful advancement in vascularized muscle tissue engineering.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148677117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gravity-driven microfluidic synthesis of lipid nanoparticles via vibrating sharp-tip mixing.","authors":"Toktam Godary, Zhengru Liu, Peng Li","doi":"10.1088/1758-5090/ae94b4","DOIUrl":"10.1088/1758-5090/ae94b4","url":null,"abstract":"<p><p>Lipid nanoparticles (LNPs) are widely used in nanomedicine, yet their energy-efficient manufacture with precise control over particle uniformity remains challenging. Here we report a gravity-driven microfluidic platform that integrates acoustically actuated sharp-tip mixing to enable high-throughput LNPs synthesis without the need for active pumps. By optimizing microchannel geometry, actuation conditions, and the spatial positioning of the mixing vortex, we achieve stable gravity-driven flow and complete mixing at total flow rates (TFRs) of up to 5 ml min^-1. The optimized system produces LNPs with tunable sizes below 60 nm, polydispersity <0.3, and high production yields. We further show that nanoparticle uniformity is governed not only by mixing strength but also by where mixing and nucleation occur within the microchannel. These findings establish gravity-driven sharp-tip microfluidics as a scalable and energy-efficient strategy for controlled nanoparticle manufacturing, offering a practical route toward greener nanomedicine production.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13478737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148700713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organoids in endometrial diseases: from bench to bedside.","authors":"Dan Yang, Jingyi Zhang, Fengqing Lv, Shuyi Ma, Jiao Cui, Dan Liu","doi":"10.1088/1758-5090/ae93ff","DOIUrl":"10.1088/1758-5090/ae93ff","url":null,"abstract":"<p><p>The endometrium, a dynamic tissue of paramount importance to female reproductive health, undergoes cyclic remodeling governed by hormonal signals. Endometrial diseases have a relatively high incidence rate and exert a considerable influence on women's quality of life; however, their underlying mechanisms remain incompletely understood. Endometrial organoids (EOs) possess considerable potential to replicate tissue-specific functions and disease phenotypes. This review begins by outlining the physiological and pathological basis of the endometrium, then systematically reviews the latest progress in EOs research, emphasizing the importance of developing precise organoid models. We further discuss strategies for organoid construction, key characteristics, and functional validation methods. Applications of EOs in modeling endometrial diseases, drug screening, and personalized therapy are summarized. Finally, we address current challenges in the field and suggest future directions. Despite challenges related to standardization and clinical translation, continued refinement of EOs systems will deepen our understanding of endometrial biology and accelerate the development of targeted therapies for endometrial diseases.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148668393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-throughput design of 3D hybrid stem cell spheroids loaded with zinc-doped urchin-like hydroxyapatite and their osteopromotive bionics.","authors":"Hongmei Liu, Lei Zhou, Jianxin Ye, Dianwen Song, Ran Cao, Ruichun Luo, Liping Zhu, Meifang Zhu","doi":"10.1088/1758-5090/ae9403","DOIUrl":"10.1088/1758-5090/ae9403","url":null,"abstract":"<p><p>Stem cell spheroids, as an advanced platform in cell therapy, have shown great potential for improving therapeutic efficacy through enhanced cell-cell and cell-microenvironment interactions. Meanwhile, zinc-based biomaterials are attracting increasing attention for their biological functions. However, their roles in modulating stem cell spheroids remain largely unclear. In this study, we synthesized zinc-doped urchin-like hydroxyapatite (Zn-uHA) via a hydrothermal method and verified its long-term antibacterial activity. Utilizing microwell array technology, we conducted 3D cell culture of human dental pulp stem cells (hDPSCs) with Zn-uHA, thereby achieving high-throughput and precise fabrication of 3D hDPSCs/Zn-uHA hybrid stem cell spheroids. Compared with pure cell spheroids, hDPSCs/Zn₃-uHA hybrid spheroids demonstrated significantly enhanced cell migration and osteogenic differentiation, with osteogenesis-related gene expression upregulated 2.7-fold.<i>In vivo</i>studies further confirmed that the hybrid cell spheroids exhibited favorable biocompatibility, with no evident inflammatory response or immune rejection, and enabled prolonged retention at the implantation site. Additionally, they significantly promoted neovascularization and new bone formation. These findings suggest that Zn-uHA-integrated stem cell spheroids represent a promising strategy for enhancing both antibacterial and osteogenic performance, offering potential applications in bone tissue engineering, particularly in infection-prone clinical scenarios such as orthopedics and dentistry.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148668373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling the synovium on a chip for studying the interactions between lymphatic vasculature and synoviocytes in rheumatoid arthritis.","authors":"Samantha E Kraus, Issahy Cano, Soo Jin Kim, Esak Lee","doi":"10.1088/1758-5090/ae9348","DOIUrl":"10.1088/1758-5090/ae9348","url":null,"abstract":"<p><p>Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disorder that afflicts the synovial lining of joints and manifests in reduced range of motion, pain, swelling, and numerous other complications with no effective cure. In recent years, attention has shifted toward eradicating the source of synovial autoimmunity and inflammation, with a key focus on the synovial-draining lymphatics. However, very few<i>in vitro</i>models of the synovial microenvironment have been developed to date, and none yet include the synovial lymphatics. We therefore create a microfluidic chip device that models the synovial-draining lymphatics within the subintimal synovium microenvironment. Functional assays on our synovium-on-chip demonstrate increased lymphatic permeability and decreased drainage under RA inflammation compared to healthy controls, accompanied by increased lymphatic endothelial cell (LEC) junctional disruption and altered LEC phenotype following interaction with fibroblast-like synoviocytes. We identify overexpression of chitinase-3 like-protein-1 from RA patient-derived synoviocytes as a key target in inducing junctional loosening and lymphatic dysfunction, which is reversed in our microfluidic chip and in vivo mouse models by neutralizing antibody inhibition. Our novel synovium-on-chip model can provide a physiologically accurate representation of lymphatic drainage and activity under disease conditions, informing tissue engineering, drug testing, and high-throughput screening for RA.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13469983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148648247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estrogen-loaded scaffolds promote thyrocyte proliferation and endogenous antioxidant expression while preserving functional stability.","authors":"Maria Heim, Ella-Louise Handley, Yunxi Gao, Aimee Mandel, Elaine Emmerson, Anthony Callanan","doi":"10.1088/1758-5090/ae8bdb","DOIUrl":"10.1088/1758-5090/ae8bdb","url":null,"abstract":"<p><p>Loss of functional thyrocytes following thyroid injury or radiotherapy remains a significant clinical challenge, motivating development of biomaterial-based strategies to support thyroid cell survival and function. 17-Estradiol (E2) has been shown to promote thyrocyte proliferation and antioxidant responses, although systemic or bolus delivery may induce cytotoxicity and raises concerns regarding long-term pathological effects. Here, electrospun polycaprolactone (PCL) scaffolds incorporating varying concentrations of E2 were developed as a localised hormone delivery platform for thyroid epithelial cells. E2 incorporation altered scaffold mechanical behaviour, hydrophilicity, and porosity in a concentration-dependent manner while preserving fibre morphology, crystallinity, and thermal stability. Scaffold-mediated E2 delivery supported sustained thyrocyte viability and proliferation compared with bolus E2 exposure, with intermediate E2 concentrations producing the most favourable biological response. Morphological analysis demonstrated extensive cell spreading and organised cytoskeletal structures without features associated with apoptosis. Although E2-loaded scaffolds did not exhibit direct radical scavenging activity, they promoted upregulation of antioxidant-associated genes, indicating indirect modulation of cellular redox homeostasis. Thyroid-specific functional markers associated with hormone synthesis and maintenance of the differentiated thyroid phenotype were largely preserved across scaffold groups during the 14 d culture period. Furthermore, no detectable<i>BRAF</i>V600E or V600K mutations were identified under any condition tested. Collectively, these findings demonstrate that electrospun E2@PCL scaffolds provide a tunable microenvironment capable of supporting thyrocyte survival, proliferation, and functional maintenance, highlighting their potential for thyroid tissue engineering applications targeting radiation-induced thyroid injury.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Degradable porous PLGA/PCL membrane enable a lung alveoli-on-a-chip for modeling particulate-induced alveolar injury.","authors":"Jae-Won Choi, Sikandar Azam, MyAnh Hisaeda, Shimin Liu, Si-Yang Zheng","doi":"10.1088/1758-5090/ae9402","DOIUrl":"10.1088/1758-5090/ae9402","url":null,"abstract":"<p><p>Understanding how airborne particulates disrupt the alveolar barrier requires<i>in vitro</i>systems that recapitulate both the structure and transport properties of the lung air-blood interface. Here, we report a biodegradable lung alveoli-on-a-chip enabled by porous poly(lactic-co-glycolic acid)/polycaprolactone (PLGA/PCL) membranes with an interconnected porous architecture generated via porogen-assisted phase separation process. The membrane exhibits tunable degradation behavior, allowing progressive increases in surface porosity (∼40%) and reduction in thickness (∼3<i>µ</i>m) during culture, while PCL maintains mechanical integrity under dynamic conditions. These degradation-driven structural changes regulate membrane transport properties, leading to enhanced permeability and supporting the formation of a functional epithelial-endothelial barrier under air-liquid interface culture with breathing-mimetic cycling strain. Primary human alveolar epithelial and microvascular endothelial cells formed confluent, junctional monolayers on opposing membrane surfaces, exhibiting stable barrier function and high viability throughout the culture period. As a functional application, the platform was used to assess diesel particulate matter (DPM)-induced alveolar injury. Apical exposure to DPM induced dose-dependent cytotoxicity, increased barrier permeability, elevated reactive oxygen species, and DNA damage in both epithelial and endothelial layers, demonstrating trans-barrier propagation of particulate-induced injury. Pharmacological modulation with roflumilast-N-oxide, a phosphodiesterase-4 inhibitor, selectively attenuated oxidative stress and inflammatory responses, with limited effects on barrier integrity. Together, this work establishes degradable PLGA/PCL membranes as tunable interface materials for lung-on-a-chip systems, where structural evolution during degradation directly governs transport and barrier function. The resulting platform provides a physiologically relevant approach for studying particulate toxicity and therapeutic modulation at the alveolar interface.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148668337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a gut-on-a-chip microfluidic device with three-dimensionally printed human intestinal tissue for studying human-microbe interactions.","authors":"Lindy K Jang, Claire J Robertson, Michael G Triplett, Aubree Hinckley, Monica L Moya, Car Reen Kok, Mariam V Mohagheghi, Tracy M Weisenberger, Feliza A Bourguet, Erica S Bowers, Michael Morrison, Yaqing Wang, Aleksandr Noy, Ju An Park, Nicholas A Be, William F Hynes","doi":"10.1088/1758-5090/ae8ccc","DOIUrl":"10.1088/1758-5090/ae8ccc","url":null,"abstract":"<p><p>The human small intestinal epithelium features villi protruding into the gut lumen, and crypts invaginating toward the gut exterior, forming a microarchitecture critical for intestinal homeostasis and renewal. Reproducing this complex geometry at physiological dimensions and pliability, while achieving cell compatibility, remains challenging. Here, we developed three-dimensionally (3D) printed gelatin methacryloyl (GelMA) crypt-villus scaffolds and a gut-on-a-chip microfluidic device integrating dynamic fluid flow control, oxygen/pH regulation, and continuously sampled gut effluent collection. Using our custom biological projection micro-stereolithography (BioPSL) system, we fabricated physiologically relevant crypt-villus scaffolds with physiological dimensions and softness within 30 min. We demonstrated that microbial transglutaminase (TG) enzyme could stably link proteins to GelMA, significantly improving Caco-2 cell adhesion to the GelMA surface. Moreover, we show stable protein density gradients could be created by allowing the mixture of TG and proteins to diffuse into hydrogels. Human intestinal cells seeded on 3D printed intestinal tissues exhibited robust adhesion, proliferation, and maturation into an apico-basal polarized monolayer. By integrating the crypt-villus scaffolds into the microfluidic platform, we demonstrated its potential for co-culturing epithelial cells with gut-relevant microbes, enabling monitoring of oxygen and pH levels and analysis of microbial growth during co-culture and assessment of cell viability afterward. This innovative platform holds promise for investigating human-microbiome interactions, advancing disease diagnosis/prevention, and facilitating drug screening applications.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148519963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scalable subunit-based strategy to fabricate autologous vaginal tissue grafts.","authors":"Pamela A Moalli, Jeffrey R Morgan, Vivian Sung, Hannah Johnson, Katerina St Angelo, Abigail Shaffer, Madeleine Marx, Vera Fonseca, John Murphy Iii, Gabrielle King, Temara Stransky, Jonathon Vande Geest, Katrina Knight, Marianna Alperin, David D Rahn, Holly E Richter, Ariana Smith, Alison Weidner, Marie Gantz, Donna Mazloomdoost","doi":"10.1088/1758-5090/ae8d5c","DOIUrl":"10.1088/1758-5090/ae8d5c","url":null,"abstract":"<p><p>Vaginal reconstruction is limited by the lack of biomaterials that replicate the structure, biomechanics, and biochemistry of the native tissues. Synthetic meshes, xenografts, and autologous skin or bowel grafts are hindered by their immunogenicity, poor integration, and non-physiological properties. We report a scalable platform for the fabrication of patient-specific living vaginal grafts from autologous fibroblasts. Using scaffold-free micromolding and automated assembly, fibroblasts from small full-thickness vaginal biopsies self-assembled into a collagen-rich, highly aligned extracellular matrix. To improve mechanical integrity, we create twisted subunit assemblies that displayed highly aligned collagen, dense cellularity, and a predominantly quiescent fibroblast phenotype with minimal myofibroblast activation. This autologous tissue-specific construct addresses the shortcomings of current materials and offers a customizable and biocompatible solution for regenerative gynecology. By combining tissue specificity, immunologic safety, and modular scalability, this approach has the potential to transform surgical options for congenital anomalies, post-oncologic reconstruction, fistula repair, and pelvic organ prolapse.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148534966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}