Biofabrication最新文献_第10页

Valorization of crop by-products into bio-based dental materials: advancements and prospects. 农作物副产品转化为生物基牙科材料：进展与展望。

IF 8.2 2区医学

Biofabrication Pub Date : 2025-02-18 DOI: 10.1088/1758-5090/ada736

Marcin Mikulewicz, Katarzyna Chojnacka

引用次数: 0

Rational design of 3D-printed scaffolds for breast tissue engineering using structural analysis. 基于结构分析的乳腺组织工程3d打印支架的合理设计

IF 8.2 2区医学

Biofabrication Pub Date : 2025-02-17 DOI: 10.1088/1758-5090/adaf5a

Sharon Kracoff-Sella, Idit Goldfracht, Asaf Silverstein, Shira Landau, Lior Debbi, Rita Beckerman, Hagit Shoyhat, Yifat Herman-Bachinsky, Gali Guterman-Ram, Inbal Michael, Rita Shuhmaher, Janette Zavin, Ronen Ben Horin, Dana Egozi, Shulamit Levenberg

{"title":"Rational design of 3D-printed scaffolds for breast tissue engineering using structural analysis.","authors":"Sharon Kracoff-Sella, Idit Goldfracht, Asaf Silverstein, Shira Landau, Lior Debbi, Rita Beckerman, Hagit Shoyhat, Yifat Herman-Bachinsky, Gali Guterman-Ram, Inbal Michael, Rita Shuhmaher, Janette Zavin, Ronen Ben Horin, Dana Egozi, Shulamit Levenberg","doi":"10.1088/1758-5090/adaf5a","DOIUrl":"10.1088/1758-5090/adaf5a","url":null,"abstract":"Best cosmetic outcomes of breast reconstruction using tissue engineering techniques rely on the scaffold architecture and material, which are currently both to be determined. This study suggests an approach for a rational design of breast-shaped scaffold architecture, in which structural analysis is implemented to predict its stiffness and adjust it to that of the native tissue. This approach can help achieve the goal of optimal scaffold architecture for breast tissue engineering. Based on specifications defined in a preliminary implantation study of a non-rationally designed scaffold, and using analytical modeling and finite element analysis, we rationally designed a polycaprolactone made, 3D-printed, highly porous, breast-shaped scaffold with a stiffness similar to the breast adipose tissue. This scaffold had an architecture of a double-shelled dome connected by pillars, with no bottom to allow direct contact of its fat graft with the host's blood vessels (shelled hemisphere adaptive design (SHAD)). To demonstrate the potential of the SHAD scaffold in breast tissue engineering, a proof-of-concept study was performed, in which SHAD scaffolds were embedded with human adipose derived mesenchymal stem cells, isolated from lipoaspirates, and implanted in nod-scid-gamma mouse model with a delayed fat graft injection. After 4 weeks of implantation, the SHAD implants were vascularized with a viable fat graft, indicating the suitability of the SHAD scaffold for breast tissue engineering.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergizing bioprinting and 3D cell culture to enhance tissue formation in printed synthetic constructs. 协同生物打印和3D细胞培养，以提高组织形成的打印合成结构。

IF 8.2 2区医学

Biofabrication Pub Date : 2025-02-14 DOI: 10.1088/1758-5090/adae37

Daniel Günther, Cédric Bergerbit, Ary Marsee, Sitara Vedaraman, Alba Pueyo Moliner, Céline Bastard, Guy Eelen, José Luis Gerardo Nava, Mieke Dewerchin, Peter Carmeliet, Rafael Kramann, Kerstin Schneeberger, Bart Spee, Laura De Laporte

{"title":"Synergizing bioprinting and 3D cell culture to enhance tissue formation in printed synthetic constructs.","authors":"Daniel Günther, Cédric Bergerbit, Ary Marsee, Sitara Vedaraman, Alba Pueyo Moliner, Céline Bastard, Guy Eelen, José Luis Gerardo Nava, Mieke Dewerchin, Peter Carmeliet, Rafael Kramann, Kerstin Schneeberger, Bart Spee, Laura De Laporte","doi":"10.1088/1758-5090/adae37","DOIUrl":"10.1088/1758-5090/adae37","url":null,"abstract":"Bioprinting is currently the most promising method to biofabricate complex tissuesin vitrowith the potential to transform the future of organ transplantation and drug discovery. Efforts to create such tissues are, however, almost exclusively based on animal-derived materials, such as gelatin methacryloyl, which have demonstrated efficacy in bioprinting of complex tissues. While these materials are already used in clinical applications, uncertainty about their safety still remains due to their animal origin. Alternatively, synthetic bioinks have been developed that match the printability of natural bioinks but lack their biological complexity, and thereby often fail to support cell growth and facilitate tissue formation. Additionally, most synthetic materials do not meet the mechanical demands of bioprint stable constructs while providing a suitable environment for cells to grow, limiting the number of available bioinks. To bridge this gap and synergize bioprinting and 3D cell culture, we developed a polyethylene glycol-based bioink system to promote the growth and spreading of cell spheroids that consist of human primary endothelial cells and fibroblasts. The 3D bioprinted centimeter-scale constructs have a high shape fidelity and accelerated softening to provide sufficient space for cells to grow. Adjusting the rate of degradability, induced by the integration of ester-functionalized crosslinkers in addition to protease cleavable crosslinkers into the hydrogel network, improves the growth of spheroids in larger printed hydrogel constructs containing an interconnected channel structure. The perfusable constructs enable extensive spheroid sprouting and the formation of a cellular network upon fusion of sprouts as initial steps toward tissue formation with the potential for clinical translation.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purified adipose tissue-derived extracellular vesicles facilitate adipose organoid vascularization through coordinating adipogenesis and angiogenesis. 纯化脂肪组织来源的细胞外囊泡通过协调脂肪生成和血管生成促进脂肪类器官血管化。

IF 8.2 2区医学

Biofabrication Pub Date : 2025-02-14 DOI: 10.1088/1758-5090/adb2e7

Congxiao Zhu, Zonglin Huang, Hongru Zhou, Xuefeng Han, Lei Li, Ningbei Yin

{"title":"Purified adipose tissue-derived extracellular vesicles facilitate adipose organoid vascularization through coordinating adipogenesis and angiogenesis.","authors":"Congxiao Zhu, Zonglin Huang, Hongru Zhou, Xuefeng Han, Lei Li, Ningbei Yin","doi":"10.1088/1758-5090/adb2e7","DOIUrl":"10.1088/1758-5090/adb2e7","url":null,"abstract":"One of the major challenges in the way of better fabricating vascularized adipose organoids is the destructive effect of adipogenic differentiation on preformed vasculature, which probably stems from the discrepancy between thein vivophysiological microenvironment and thein vitroculture conditions. As an intrinsic component of adipose tissue (AT), adipose tissue-derived extracellular vesicles (AT-EVs) have demonstrated both adipogenic and angiogenic ability in recent studies. However, whether AT-EVs could be employed to coordinate the angiogenesis and adipogenesis in the vascularization of adipose organoids remains largely unexplored. Herein, we present an efficient method for isolating higher-purity AT-EV preparations from lipoaspirates, and verify the superiority of AT-EV preparations' angiogenic and adipogenic capabilities over those from unpurified lipoaspirates. Next, in the spheroid culture model, it was discovered that the addition of AT-EVs could effectively improve the aggregation through enhancing intercellular adhesion of monoculture spheroids composed of human umbilical vascular endothelial cells (HUVECs), and helped produce vascularized adipose organoids with proper lipolysis and glucose uptake ability in the coculture spheroids comprised of adipose-derived stem cells (ADSCs) and HUVECs. Subsequently, it was observed that AT-EVs could exert a retaining effect on the vasculature of prevascularized coculture spheroids cultured in an adipogenic environment, compared to the reduced vascular networks where AT-EVs were absent. Altogether, these results indicate that AT-EVs, by means of releasing bioactive molecules that emulate thein vivomicroenvironment, can modify non-replicativein vitromicroenvironments, coordinatein vitroadipogenesis and angiogenesis, and facilitate the fabrication of vascularized adipose organoids.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current progress ofin vitrovascular models on microfluidic chips. 微流控芯片体外血管模型的研究进展。

IF 8.2 2区医学

Biofabrication Pub Date : 2025-02-13 DOI: 10.1088/1758-5090/adb182

Ran Wang, Hangyu Zhang, Shijun Li, Peishi Yan, Shuai Shao, Bo Liu, Na Li

{"title":"Current progress ofin vitrovascular models on microfluidic chips.","authors":"Ran Wang, Hangyu Zhang, Shijun Li, Peishi Yan, Shuai Shao, Bo Liu, Na Li","doi":"10.1088/1758-5090/adb182","DOIUrl":"10.1088/1758-5090/adb182","url":null,"abstract":"The vascular tissue, as an integral component of the human circulatory system, plays a crucial role in retaining normal physiological functions within the body. Pathologies associated with the vasculature, whether direct or indirect, also constitute significant public health concerns that afflict humanity, leading to the wide studies on vascular physiology and pathophysiology. Given the precious nature of human derived vascular tissue, substantial efforts have been dedicated to the construction of vascular models. Due to the high cost associated with animal experimentation and the inability to directly translate results to human, there is an increasing emphasis on the use of primary human cells for the development ofin vitrovascular models. For instance, obtaining an ApoE-/-mouse model for atherosclerosis research typically requires feeding a high-fat diet for over 10 weeks, whereasin vitrovascular models can usually be formed within 2 weeks. With advancements in microfluidic technology,in vitrovascular models capable of precisely emulating the hemodynamic environment within human vessels are becoming increasingly sophisticated. Microfluidic vascular models are primarily constructed through two approaches: (1) directly constructing the vascular models based on the three-layer structure of the vascular wall; (2) co-culture of endothelial cells and supporting cells within hydrogels. The former is effective to replicate vascular tissue structure mimicking vascular wall, while the latter has the capacity to establish microvascular networks. This review predominantly presents and discusses recent advancements in template design, construction methods, and potential applications of microfluidic vascular models based on polydimethylsiloxane (PDMS) soft lithography. Additionally, some refined methodologies addressing the limitations of conventional PDMS-based soft lithography techniques are also elaborated, which might hold profound importance in the field of vascular tissue engineering on microfluidic chips.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced osteogenic differentiation in hyaluronic acid methacrylate (HAMA) matrix: a comparative study of hPDC and hBMSC spheroids for bone tissue engineering. 甲基丙烯酸透明质酸（HAMA）基质增强成骨分化：骨组织工程中hPDC和hBMSC球体的比较研究。

IF 8.2 2区医学

Biofabrication Pub Date : 2025-02-13 DOI: 10.1088/1758-5090/adb2e6

Ane Albillos Sanchez, Filipa Castro Teixeira, Paula Casademunt, Ivo Beeren, Lorenzo Moroni, Carlos Mota

{"title":"Enhanced osteogenic differentiation in hyaluronic acid methacrylate (HAMA) matrix: a comparative study of hPDC and hBMSC spheroids for bone tissue engineering.","authors":"Ane Albillos Sanchez, Filipa Castro Teixeira, Paula Casademunt, Ivo Beeren, Lorenzo Moroni, Carlos Mota","doi":"10.1088/1758-5090/adb2e6","DOIUrl":"10.1088/1758-5090/adb2e6","url":null,"abstract":"Bone tissue engineering (BTE) seeks to overcome the limitations of traditional bone repair methods, such as autografts and allografts, which are often limited by availability, donor-site morbidity, immune rejection, and infection risks. Recent advancements have highlighted the potential of spheroids or microtissues as building blocks for BTE. This study aimed to investigate the osteogenic differentiation of spheroids formed from human periosteum-derived cells (hPDCs) and bone marrow-derived mesenchymal stromal cells (hBMSCs) in a hyaluronic acid methacrylate (HAMA) matrix, using encapsulation and extrusion bioprinting methods. Results showed significant morphological changes, high viability, and osteogenic differentiation of spheroids from hPDCs or hBMSCs in three-dimensional HAMA environments. Notably, hPDC spheroids demonstrated higher mineralization capabilities and superior hydrogel colonization than hBMSC spheroids. These findings reveal the potential of HAMA bioink containing hPDC spheroids to produce mineralized bone grafts using a bioprinting approach.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioinks for engineering gradient-based osteochondral and meniscal tissue substitutes: a review. 基于梯度的工程骨软骨和半月板组织替代物的生物墨水：综述。

IF 8.2 2区医学

Biofabrication Pub Date : 2025-02-13 DOI: 10.1088/1758-5090/adb0f4

Mahdieh Heydarigoojani, Maryam Farokhi, Sara Simorgh

引用次数: 0

Advancement of 3D biofabrication in repairing and regeneration of cartilage defects. 三维生物制造在软骨缺损修复与再生中的研究进展。

IF 8.2 2区医学

Biofabrication Pub Date : 2025-02-12 DOI: 10.1088/1758-5090/ada8e1

Zenghui Zheng, Dongmei Yu, Haoyu Wang, Hao Wu, Zhen Tang, Qi Wu, Pengfei Cao, Zhiyuan Chen, Hai Huang, Xiaokang Li, Chaozong Liu, Zheng Guo

{"title":"Advancement of 3D biofabrication in repairing and regeneration of cartilage defects.","authors":"Zenghui Zheng, Dongmei Yu, Haoyu Wang, Hao Wu, Zhen Tang, Qi Wu, Pengfei Cao, Zhiyuan Chen, Hai Huang, Xiaokang Li, Chaozong Liu, Zheng Guo","doi":"10.1088/1758-5090/ada8e1","DOIUrl":"10.1088/1758-5090/ada8e1","url":null,"abstract":"Three-dimensional (3D) bioprinting, an additive manufacturing technology, fabricates biomimetic tissues that possess natural structure and function. It involves precise deposition of bioinks, including cells, and bioactive factors, on basis of computer-aided 3D models. Articular cartilage injuries, a common orthopedic issue. Current repair methods, for instance microfracture procedure (MF), autologous chondrocyte implantation (ACI), and osteochondral autologous transfer surgery have been applied in clinical practice. However, each procedure has inherent limitation. For instance, MF surgery associates with increased subchondral cyst formation and brittle subchondral bone. ACI procedure involves two surgeries, and associate with potential risks infection and delamination of the regenerated cartilage. In addition, chondrocyte implantation's efficacy depends on the patient's weight, joint pathology, gender-related histological changes of cartilage, and hormonal influences that affect treatment and prognosis. So far, it is a still a grand challenge for achieving a clinical satisfactory in repairing and regeneration of cartilage defects using conditional strategies. 3D biofabrication provide a potential to fabricate biomimetic articular cartilage construct that has shown promise in specific cartilage repair and regeneration of patients. This review reported the techniques of 3D bioprinting applied for cartilage repair, and analyzed their respective merits and demerits, and limitations in clinical application. A summary of commonly used bioinks has been provided, along with an outlook on the challenges and prospects faced by 3D bioprinting in the application of cartilage tissue repair. It provided an overall review of current development and promising application of 3D biofabrication technology in articular cartilage repair.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142963690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-step bioprinting of endothelialized, self-supporting arterial and venous networks. 一步生物打印的内皮化，自我支持的动脉和静脉网络。

IF 8.2 2区医学

Biofabrication Pub Date : 2025-02-11 DOI: 10.1088/1758-5090/adab26

Betty Cai, David Kilian, Sadegh Ghorbani, Julien G Roth, Alexis J Seymour, Lucia G Brunel, Daniel Ramos Mejia, Ricardo J Rios, Isabella M Szabo, Sean Chryz Iranzo, Andy Perez, Rameshwar R Rao, Sungchul Shin, Sarah C Heilshorn

{"title":"One-step bioprinting of endothelialized, self-supporting arterial and venous networks.","authors":"Betty Cai, David Kilian, Sadegh Ghorbani, Julien G Roth, Alexis J Seymour, Lucia G Brunel, Daniel Ramos Mejia, Ricardo J Rios, Isabella M Szabo, Sean Chryz Iranzo, Andy Perez, Rameshwar R Rao, Sungchul Shin, Sarah C Heilshorn","doi":"10.1088/1758-5090/adab26","DOIUrl":"10.1088/1758-5090/adab26","url":null,"abstract":"Advances in biofabrication have enabled the generation of freeform perfusable networks mimicking vasculature. However, key challenges remain in the effective endothelialization of these complex, vascular-like networks, including cell uniformity, seeding efficiency, and the ability to pattern multiple cell types. To overcome these challenges, we present an integrated fabrication and endothelialization strategy to directly generate branched, endothelial cell-lined networks using a diffusion-based, embedded 3D bioprinting process. In this strategy, a gelatin microparticle sacrificial ink delivering both cells and crosslinkers is extruded into a crosslinkable gel precursor support bath. A self-supporting, perfusable structure is formed by diffusion-induced crosslinking, after which the sacrificial ink is melted to allow cell release and adhesion to the printed lumen. This approach produces a uniform cell lining throughout networks with complex branching geometries, which are challenging to uniformly and efficiently endothelialize using conventional perfusion-based approaches. Furthermore, the biofabrication process enables high cell viability (>90%) and the formation of a confluent endothelial layer providing vascular-mimetic barrier function and shear stress response. Leveraging this strategy, we demonstrate for the first time the patterning of multiple endothelial cell types, including arterial and venous cells, within a single arterial-venous-like network. Altogether, this strategy enables the fabrication of multi-cellular engineered vasculature with enhanced geometric complexity and phenotypic heterogeneity.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent trends in embedded 3D bioprinting of vascularized tissue constructs. 血管化组织结构的嵌入式3D生物打印的最新趋势。

IF 8.2 2区医学

Biofabrication Pub Date : 2025-02-10 DOI: 10.1088/1758-5090/adafdd

Won-Woo Cho, Wonbin Park, Dong-Woo Cho

{"title":"Recent trends in embedded 3D bioprinting of vascularized tissue constructs.","authors":"Won-Woo Cho, Wonbin Park, Dong-Woo Cho","doi":"10.1088/1758-5090/adafdd","DOIUrl":"10.1088/1758-5090/adafdd","url":null,"abstract":"3D bioprinting technology offers significant advantages in the fabrication of tissue and organ structures by allowing precise layer-by-layer patterning of cells and various biomaterials. However, conventional bioinks exhibit poor mechanical properties, which limit their use in the fabrication of large-scale vascularized tissue constructs. To address these limitations, recent studies have focused on the development of rapidly crosslinkable bioinks through chemical modification. These enable rapid crosslinking within minutes, offering substantial advantages for engineering large-scale tissue constructs. Nevertheless, challenges remain in the production of constructs that fully incorporate the complex vascular networks inherent to native tissues. Recently, embedded bioprinting technique, which involves the direct writing of bioink into a support bath that provides physical support, has gained significant attention for enabling the freeform fabrication of 3D structures. This method has been extensively studied and offers the advantage of fabricating structures ranging from tissue constructs with simple vascular channels to complex structures containing multiscale vascular networks. This review presents an overview of the various materials utilized in embedded bioprinting and elucidates the rheological properties of these materials. Furthermore, it examines the current research trends in the biofabrication of vascularized tissue constructs using embedded bioprinting techniques, along with their associated limitations. The review concludes by proposing areas for future improvement, specifically addressing material and biofabrication approaches as well as bioprinting systems.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0