Biofabrication最新文献

{"title":"Optimizing extrusion-based 3D bioprinting of plant cells with enhanced resolution and cell viability.","authors":"Dezhi Zhou, Peixi Li, Shuang Yu, Zhenhua Cui, Tao Xu, Liliang Ouyang","doi":"10.1088/1758-5090/adada1","DOIUrl":"10.1088/1758-5090/adada1","url":null,"abstract":"<p><p>3D bioprinting of plant cells has emerged as a promising technology for plant cell immobilization and related applications. Despite the numerous progress in mammalian cell printing, the bioprinting of plant cells is still in its infancy and needs further investigation. Here, we present a systematic study on optimizing the 3D bioprinting of plant cells, using carrots as an example, towards enhanced resolution and cell viability. We mainly investigated the effects of cell cluster forms and nozzle size on the rheological, extrusion, and printability properties of plant cell bioinks, as well as on the resultant cell viability and growth. We found that when the printing nozzle is larger than 85% of the cell clusters embedded in the bioink, smooth extrusion and good printability can be achieved together with considerable cell viability and long-term growth. Specifically, we optimized a bioink composited with suspension-cultured carrot cells, which exhibited better uniformity, smoother extrusion, and higher cell viability over 1 month culture compared to those with the regular callus or fragmented callus. This work provides a practical guideline for optimizing plant cell bioprinting from the bioink development to the printing outcome assessment. It highlights the importance of selecting a matched nozzle and cell cluster and might provide insights for a better understanding and exploitation of plant cell bioprinting.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microchannel fabrication on bio-grade Nitinol SMA by<i>μ</i>-ED milling process using sustainable oil for improving the machining performance and biocompatibility.","authors":"Satish Chaurasia, Kishore Debnath","doi":"10.1088/1758-5090/adaaa2","DOIUrl":"10.1088/1758-5090/adaaa2","url":null,"abstract":"<p><p>The process of micromachining has garnered attention for its ability to create three-dimensional tiny features, particularly in ultra-hard and exotic materials. The present work investigates the effect of different parameters of the<i>μ</i>-ED milling, such as pulse on time (<i>T</i>_on), pulse off time (<i>T</i>_off), voltage (<i>V</i>), and tool rotation (TR) on the dimensional deviation (DD), material removal rate (MRR), surface roughness (Ra), and machined surface characteristics (analyzed by EDS and FESEM). The sesame oil as dielectric and tungsten-copper as tool electrodes were used to maintain the accuracy and improve the machinability of bio-grade Nitinol shape memory alloy (SMA). Response surface methodology (RSM) and genetic algorithms (GAs) were used to optimize the various input parameters of the<i>μ</i>-ED milling process. Artificial neural network was combined with GA to find the best parametric combination for microchannel fabrication. The cytotoxicity test was also performed on the machined surface to analyze the biocompatibility of the machined surface. It was found that the cell viability of Nitinol SMA was improved by 85.11% after machining at the optimum condition. The highest MRR was found to be 0.076 gm min^-1, and the lowest DD and Ra were found to be 16.47<i>μ</i>m and Ra 0.387<i>μ</i>m, respectively.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospun robust, biodegradable, bioactive, and nanostructured sutures to accelerate the chronic wound healing.","authors":"Yiran Li, Hongxing Xu, Wenwen Zhao, Li Zhang, Shaohua Wu","doi":"10.1088/1758-5090/adacaf","DOIUrl":"10.1088/1758-5090/adacaf","url":null,"abstract":"<p><p>The design and development of advanced surgical sutures with appropriate structure and abundant bio-functions are urgently required for the chronic wound closure and treatment. In this study, an integrated technique routine combining modified electrospinning with hot stretching process was proposed and implemented to fabricate poly(L-lactic acid) (PLLA) nanofiber sutures, and the Salvia miltiorrhiza Bunge-Radix Puerariae herbal compound (SRHC) was encapsulated into PLLA nanofibers during the electrospinning process to enrich the biofunction of as-generated sutures. All the PLLA sutures loading without or with SRHC were found to exhibit bead-free and highly-aligned nanofiber structure. The addition of SRHC was found to have no significant influences on the fiber morphology, diameter, and the crystallinity of as-prepared PLLA sutures. Importantly, all the SRHC-contained PLLA nanofiber sutures possessed excellent tensile and knot strength, which were of significant importance for the surgical suture applications. Besides, the antioxidant and anti-inflammatory properties of these sutures obviously enhanced with the increasing of SRHC concentration. Furthermore, the<i>in vitro</i>cell tests illustrated that the high fiber orientation of the sutures was able to efficiently induce the human dermal fibroblasts (HDFs) to migrate in a rapid manner, and the sutures loaded with high content of SRHC could significantly promote the attachment and proliferation of HDFs in comparison. The<i>in vivo</i>diabetic mouse model experiments revealed that all the as-developed PLLA sutures could effectively close the wound, but the PLLA sutures containing high content of SRHC could dramatically promote the wound healing with high quality by shortening the healing time, improving the collagen deposition, neovascularization, and the regeneration of hair follicles, especially compared with commercial polyester (PET) suture. This study offers a simple and easily-handling strategy to develop robust, biodegradable, bioactive, and nanostructured PLLA sutures, which shows huge potential for the treatment of hard-to-heal diabetic wounds.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In-situ</i>quality monitoring during embedded bioprinting using integrated microscopy and classical computer vision.","authors":"Vasileios Sergis, Daniel Kelly, Ankita Pramanick, Graham Britchfield, Karl Mason, Andrew C Daly","doi":"10.1088/1758-5090/adaa22","DOIUrl":"10.1088/1758-5090/adaa22","url":null,"abstract":"<p><p>Despite significant advances in bioprinting technology, current hardware platforms lack the capability for process monitoring and quality control. This limitation hampers the translation of the technology into industrial GMP-compliant manufacturing settings. As a key step towards a solution, we developed a novel bioprinting platform integrating a high-resolution camera for<i>in-situ</i>monitoring of extrusion outcomes during embedded bioprinting. Leveraging classical computer vision and image analysis techniques, we then created a custom software module for assessing print quality. This module enables quantitative comparison of printer outputs to input points of the computer-aided design model's 2D projections, measuring area and positional accuracy. To showcase the platform's capabilities, we then investigated compatibility with various bioinks, dyes, and support bath materials for both 2D and 3D print path trajectories. In addition, we performed a detailed study on how the rheological properties of granular support hydrogels impact print quality during embedded bioprinting, illustrating a practical application of the platform. Our results demonstrated that lower viscosity, faster thixotropy recovery, and smaller particle sizes significantly enhance print fidelity. This novel bioprinting platform, equipped with integrated process monitoring, holds great potential for establishing auditable and more reproducible biofabrication processes for industrial applications.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic development and bioprinting of novel nanostructured multi-material bioinks for bone tissue engineering.","authors":"Jannika T Korkeamäki, Ahmad Rashad, Miina Ojansivu, Jennika Karvinen, Janne T Koivisto, Kristin Syverud, Minna Kellomäki, Susanna Miettinen, Kamal Mustafa","doi":"10.1088/1758-5090/ada63b","DOIUrl":"10.1088/1758-5090/ada63b","url":null,"abstract":"<p><p>A functional bioink with potential in bone tissue engineering must be subjected to critical investigation throughout its intended lifespan. The aim of this study was to develop alginate-gelatin-based (Alg-Gel) multicomponent bioinks systematically and to assess the short- and long-term exposure responses of human bone marrow stromal cells (hBMSCs) printed within these bioinks with and without crosslinking.<u>The first generation of bioinks</u>was established by incorporating a range of cellulose nanofibrils (CNFs), to evaluate their effect on viscosity, printability and cell viability. Adding CNFs to Alg-Gel solution increased viscosity and printability without compromising cell viability. In<u>the second generation of bioinks</u>, the influence of nano-hydroxyapatite (nHA) on the performance of the optimized Alg-Gel-CNF formulation was investigated. The addition of nHA increased the viscosity and improved printability, and an adjustment in alginate concentration improved the stability of the structures in long-term culture. The third generation bioink incorporated RGD-functionalized alginate to support cell attachment and osteogenic differentiation. The optimized bioink composition exhibited improved printability, structural integrity in long-term culture and high hBMSC viability. In addition, the final bioink composition, RGD-Alg-Gel-CNF-nHA, showed osteogenic potential: production of the osteogenic marker proteins (Runx2, OCN), enzyme (ALP), and gene expression (<i>Runx2</i>,<i>OCN</i>). A further aim of the study was to evaluate the osteogenic functionality of cells released from the structures after bioprinting. Cells were printed in two bioinks with different viscosities and incubated at 37 °C in growth medium without additional CaCl₂. This caused gelatin to dissolve, releasing the cells to attach to tissue culture plates. The results demonstrated differences in hBMSC osteogenic differentiation. Moreover, the osteogenic differentiation of the released cells was different from that of the embedded cells cultured in 3D. Thus, this systematic investigation into bioink development shows improved results through the generations and sheds light on the biological effects of the bioprinting process.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":"17 2","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pneumatic conveying inkjet bioprinting for the processing of living cells.","authors":"Justyna Bożek, Olga Kurchakova, Johanna Michel, Isabel Groß, Lena Gerhards, Yanzhen Zhang, Izabella Brand, Anja U Bräuer","doi":"10.1088/1758-5090/ada8e2","DOIUrl":"10.1088/1758-5090/ada8e2","url":null,"abstract":"<p><p>Inkjet printing techniques are often used for bioprinting purposes because of their excellent printing characteristics, such as high cell viability and low apoptotic rate, contactless<i>modus operandi</i>, commercial availability, and low cost. However, they face some disadvantages, such as the use of bioinks of low viscosity, cell damage due to shear stress caused by drop ejection and jetting velocity, as well as a narrow range of available bioinks that still challenge the inkjet printing technology. New technological solutions are required to overcome these obstacles. Pneumatic conveying printing, a new type of inkjet-based printing technique, was applied for the bioprinting of both acellular and cellular fibrin-hydrogel droplets. Drops of a bioink containing 6 × 10⁶HEK293H cells ml^-1were supplied from a sterile nozzle connected to a syringe pump and deposited on a gas stream on a fibrinogen-coated glass slide, here referred to as biopaper. Fibrinogen film is the substrate of the polymerization reaction with thrombin and Ca²⁺present in the bioink. The pneumatic conveying printing technique operates on a mechanism by which drop ejection and deposition in a stream of gas occurs. The percentage of unprinted and printed dead HEK293H cells was 5 ± 2% and 7 ± 4%, respectively. Thus, compared to normal handling, pneumatic conveying printing causes only little damage to the cells. The velocity of the drop approaching the biopaper surface is below 0.2 m s^-1and does not cause any damage to the cells. The cell viability of printed cells was 93%, being an excellent value for inkjet printing technology. The HEK293H cells exhibited approximately a 24 h lag time of proliferation that was preceded by intense migration and aggregation. Control experiments proved that the cell migration and lag time were associated with the chemical nature of the fibrin hydrogel and not with cell stress.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142963718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Volumetric bioprinting of the osteoid niche.","authors":"Jessie Duquesne, Laurens Parmentier, Edward Vermeersch, Flora Lemaire, Jung Won Seo, Ruslan I Dmitriev, Sandra Van Vlierberghe","doi":"10.1088/1758-5090/adab25","DOIUrl":"10.1088/1758-5090/adab25","url":null,"abstract":"<p><p>Volumetric bioprinting has revolutionized the field of biofabrication by enabling the creation of cubic centimeter-scale living constructs at faster printing times (in the order of seconds). However, a key challenge remains: developing a wider variety of available osteogenic bioinks that allow osteogenic maturation of the encapsulated cells within the construct. Herein, the bioink exploiting a step-growth mechanism (norbornene-norbornene functionalized gelatin in combination with thiolated gelatin-GelNBNBSH) outperformed the bioink exploiting a chain-growth mechanism (gelatin methacryloyl-GelMA), as the necessary photo-initiator concentration was three times lower combined with a more than 50% reduction in required light exposure dose resulting in an improved positive and negative resolution. To mimic the substrate elasticity of the osteoid, two concentrations of the photo-initiator Li-TPO-L (1 and 10 mg ml^-1) were compared for post-curing whereby the lowest concentration was selected since it resulted in attaining the osteogenic substrate elasticity combined with excellent biocompatibility with HT1080 cells (>95%). Further physico-chemical testing revealed that the volumetric printing (VP) process affected the degradation time of the constructs with volumetric constructs degrading slower than the control sheets which could be due to the introduced fibrillar structure inherent to the VP process. Moreover, GelNBNBSH volumetric constructs significantly outperformed the GelMA volumetric constructs in terms of a 2-fold increase in photo-crosslinkable moiety conversion and a 3-fold increase in bulk stiffness of the construct. Finally, a 21-day osteogenic cell study was performed with highly viable dental pulp-derived stem cells (>95%) encapsulated within the volumetric printed constructs. Osteogenesis was greatly favored for the GelNBNBSH constructs through enhanced early (alkaline phosphatase activity) and late maturation (calcium production) osteogenic markers. After 21 d, a secretome analysis revealed a more mature osteogenic phenotype within GelNBNBSH constructs as compared to their chain-growth counterpart in terms of osteogenic, immunological and angiogenic signaling.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ameliorating macrophage pyroptosis via ANXA1/NLRP3/Caspase-1/GSDMD pathway: Ac2-26/OGP-loaded intelligent hydrogel enhances bone healing in diabetic periodontitis.","authors":"Ruoyu Li, Wenfeng Li, Yungshan Teng, Runze Li, Siyi Kong, Xin Chen, Haotian Luo, Danying Chen, Yuqing Guo, Yangqiao Qing, Hio Cheng Leong, Bingyan Guo, Meihan Chen, Zixin Pan, Shushuo Zheng, Yihong Deng, Yang Cao, Chen Zhou, Xuenong Zou, Weicai Wang","doi":"10.1088/1758-5090/ada737","DOIUrl":"10.1088/1758-5090/ada737","url":null,"abstract":"<p><p>Craniofacial bone defect healing in periodontitis patients with diabetes background has long been difficult due to increased blood glucose levels which cause overproduction of reactive oxygen species (ROS) and a low pH environment. These conditions negatively affect the function of macrophages, worsen inflammation and oxidative stress, and ultimately, hinder osteoblasts' bone repair potential. In this study, we for the first time found that annexin A1 (ANXA1) expression in macrophages was reduced in a diabetic periodontitis (DP) environment, with the activation of the NLRP3/Caspase-1/GSDMD signaling pathway, and, eventually, increased macrophage pyroptosis. Next, we have developed a new GPPG intelligent hydrogel system which was ROS and pH responsive, and loaded with Ac2-26, an ANXA1 bioactive peptide, and osteogenic peptide OGP as well. We found that Ac2-26/OGP/GPPG can effectively reduce ROS, mitigates macrophage pyroptosis via the ANXA1/NLRP3/Caspase-1/GSDMD pathway and enhanced osteogenic differentiation. The effect of Ac2-26/OGP/GPPG in regulation of pyroptosis and bone defect repair was also further validated by animal experiments on periodontitis-induced tooth loss model in diabetic rats. To conclude, our study unveils the effect of ANXA1 on macrophage pyroptosis in periodontitis patients with diabetes, based on which we introduced a promising innovative hydrogel system for improvement of bone defects repair in DP patients via targeting macrophage pyroptosis and enhancing osteogenic potential.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo vessel connection of pre-vascularised 3D-bioprinted gingival connective tissue substitutes.","authors":"Rawen Smirani, Chantal Medina, Julie Becker, Camille Dechelette, Benoit Rousseau, Jean-Christophe Fricain, Adrien Naveau","doi":"10.1088/1758-5090/adac90","DOIUrl":"https://doi.org/10.1088/1758-5090/adac90","url":null,"abstract":"<p><p>Producing oral soft tissues using tissue engineering could compensate for the disadvantages of autologous grafts (limited availability and increased patient morbidity) and currently available substitutes (shrinkage). However, there is a lack of in vitro-engineered oral tissues due to the difficulty of obtaining stable pre-vessels that connect to the host and enable graft success. The main objective was to assess the connection of pre-vascularised 3D-bioprinted gingival substitutes to the host vasculature when subcutaneously implanted in immunodeficient mice. This study produced vascularised connective tissue substitutes using extrusion-based 3D-bioprinting of primary human gingival fibroblasts (hGF) and fluorescent human endothelial cells (RFP-HUVEC) cocultures. Pre-vascularised (hGF+RFP-HUVEC -CC grids) and control (hGF only -HG grids) grids were bioprinted and pre-cultivated for 14 days to enable pre-vessels formation. In vitro vessel formation follow-up was performed. Eight-week-old female NOG mice were used for in vivo experiments. One grid per mouse was subcutaneously implanted in 20 mice (10HG/10CC). The fluorescent activity of RFP-HUVEC was monitored. Samples were retrieved at 7, 14 and 21 days. Histological, immunohistochemical, and immunofluorescent staining was performed. CC-grids formed efficient and stable pre-vessel networks within 14 days of static pre-culture. HG-grids did not contain any vessel, while CC-grids successfully connected to the host vasculature by presenting erythrocytes within the vessel lumen inside the grids starting day 7. From days 7 to 21, vessel density was stable. Human pre-vessels were present at 7 days and were progressively replaced by murine endothelial cells. This study showed that primary hGF-HUVEC co-cultures can be successfully 3D-bioprinted within biomimetic hydrogels having a close composition to the gingival connective tissue, and HUVEC organise themselves into pre-vessel networks that connect to the murine vasculature when implanted in vivo. This approach represents a promising strategy to enhance current and future oral soft tissue substitutes for prospective clinical applications.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multidimensional nanofibrous hydrogels integrated triculture system for advanced myocardial regeneration.","authors":"Dongwoo Kim, Yeong Hwan Kim, Gyubok Lee, Eun-Cheol Lee, Suk Ho Bhang, Kangwon Lee","doi":"10.1088/1758-5090/ad9cc3","DOIUrl":"10.1088/1758-5090/ad9cc3","url":null,"abstract":"<p><p>Myocardial infarction (MI) remains a leading cause of mortality worldwide, posing a significant challenge to healthcare systems. The limited regenerative capacity of cardiac tissue following MI results in chronic cardiac dysfunction, highlighting the urgent need for innovative therapeutic strategies. In this study, we explored the application of a multidimensional nanofibrous hydrogel for myocardial regeneration. We developed a composite hydrogel system by integrating fibrin, polycaprolactone (PCL), and alginate. In this system, fibrin supported cell proliferation and significantly enhanced angiogenesis when combined with human umbilical vein endothelial cells (HUVECs). PCL contributed to the alignment of encapsulated cells, improving their organization within the scaffold. Adipose-derived stem cells (ADSCs) were encapsulated within the hydrogel for their versatile regenerative potential, while C2C12 cells were incorporated for their ability to form muscle tissue. Additionally, the inclusion of alginate not only enhanced the mechanical properties of the hydrogel to better match the biomechanical demands of cardiac tissue but also played a critical role in reducing the immune response, thereby improving the system's biocompatibility. This study presents an advanced platform for myocardial regeneration using a nanofibrous hydrogel system designed to meet the dual requirements of mechanical robustness and cellular compatibility essential for cardiac tissue engineering. The triculture system, consisting of ADSCs, C2C12 cells, and HUVECs, harnesses the regenerative capabilities of each cell type, promoting both angiogenesis and tissue regeneration. This comprehensive approach addresses the immediate needs for cellular survival and integration while effectively overcoming long-term mechanical and immunological challenges.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142806067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}