Biofabrication最新文献

{"title":"An immunocompetent human kidney on-a-chip model to study renal inflammation and immune-mediated injury.","authors":"Linda Gijzen, Marleen Bokkers, Richa Hanamsagar, Thomas Olivier, Todd Burton, Laura Marlisa Tool, Mouly Fahrin Rahman, John Lowman, Virginia Savova, Terry K Means, Henriette L Lanz","doi":"10.1088/1758-5090/ad9fdf","DOIUrl":"https://doi.org/10.1088/1758-5090/ad9fdf","url":null,"abstract":"<p><p>Kidney damage and dysfunction is an emerging health issue worldwide resulting in high morbidity and mortality rates. Numerous renal diseases are recognized to be driven by the immune system. Despite this recognition, the development of targeted therapies has been challenging as knowledge of the underlying mechanism and complex interactions remains insufficient. Recent advancements in the field offer promising avenues for exploring the interplay between renal cells and immune cells and their role in the development of renal inflammation and diseases. This study describes the establishment of a human immunocompetent 3D in vitro co-culture model of the proximal tubule in a high-throughput microfluidic platform that can be used to study renal functionality and inflammatory processes. &#xD;The model incorporated RPTEC in the top compartment and HUVECs in the bottom compartment cultured under flow and in direct contact with a collagen-I ECM gel resulting in the formation of polarized tubular structures. As an immune component, human primary monocytes of different donors were added to the lumen of the endothelium. Renal inflammation was successfully induced using complement activated serum (CAS) as evident by epithelial morphological changes, increased expression of adhesion molecules, release of pro-inflammatory cytokines, and reduced epithelial viability. Realtime migratory behavior of monocytes showed increased extravasation and migration towards the ECM and Renal compartment upon exposure to CAS with donor-to-donor differences observed. Finally, immune modulatory compounds showed efficacious inhibition of monocyte migration under inflammatory conditions in the microfluidic co-culture model. &#xD;A successful co-culture model was established and can be applied to study renal functionality in health and disease but also for drug screening due to the compatibility of the platform with automation and relatively high throughput. Overall, the described proximal tubule model has high potential to fill the gap that currently exists to study renal inflammation preclinically.&#xD.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-throughput formulation of reproducible 3D cancer microenvironments for drug testing in myeloid leukemia.","authors":"M Rudzinska-Radecka, L Turos-Korgul, D Mukherjee, P Podszywalow-Bartnicka, K Piwocka, J Guzowski","doi":"10.1088/1758-5090/ad998d","DOIUrl":"10.1088/1758-5090/ad998d","url":null,"abstract":"<p><p>Leukemic microenvironment has been recognized as a factor that strongly supports the mechanisms of resistance. Therefore, targeting the microenvironment is currently one of the major directions in drug development and preclinical studies in leukemia. Despite the variety of available leukemia 3D culture models, the reproducible generation of miniaturized leukemic microenvironments, suitable for high-throughput drug testing, has remained a challenge. Here, we use droplet microfluidics to generate tens of thousands of highly monodisperse leukemic-bone marrow microenvironments within minutes. We employ gelatin methacryloyl (GelMA) as a model extracellular matrix (ECM) and tune the concentration of the biopolymer, check the impact of other components of the ECM (hyaluronic acid), cell concentration and the ratio of leukemic cells to bone marrow cells within the microbeads to establish the optimal conditions for microtissue formation. We administer model kinase inhibitor, imatinib, at various concentrations to the encapsulated leukemic microtissues, and, via comparing mono- and co-culture conditions (cancer alone vs cancer-stroma), we find that the stroma-leukemia crosstalk systematically protects the encapsulated cells against the drug-induced cytotoxicity. With that we demonstrate that our system mimics the physiological stroma-dependent protection. We discuss applicability of our model to (i) studying the role of direct- or close-contact interactions between the leukemia and bone marrow cells embedded in microscale 3D ECM on the stroma-mediated protection, and (ii) high-throughput screening of anti-cancer therapeutics in personalized leukemia therapies.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D printed PGCL@PLA/10CSPL composite scaffolds loaded with fibronectin 1 for intervertebral disc degeneration treatment.","authors":"Weilin Zhang, Siyuan Chen, Shengbang Huang, Zhencong Li, Zhongwei Wang, Zhiwen Dai, Jinguo Liang, Hongrui Rong, Qianqian Ouyang, Weixiong Guo, Yen Wei, Jinsong Wei","doi":"10.1088/1758-5090/ad998f","DOIUrl":"https://doi.org/10.1088/1758-5090/ad998f","url":null,"abstract":"<p><p>Restoration of disc height and biomechanical function is essential for intervertebral disc degeneration (IDD) treatment. Removing abnormal nucleus pulposus (NP) tissue is an important step to facilitate bony fusion during the healing process. We analyzed publicly available single-cell transcriptome data for human normal and degenerative NP to identify genes associated with NP degeneration. A novel poly(glycolide-co-caprolactone)@polylactide (PLA)-b-aniline pentamer (AP)-b-PLA/chitosan-<i>ϵ</i>-polylysine (PGCL@1PAP/10CSPL) scaffold with good biocompatibility and electroactivity was designed and fabricated as an implant for IDD treatment using 3D printing technology. The PGCL@1PAP/10CSPL scaffold exhibited superior hydrophilicity, mechanical properties, cytocompatibility, and antibacterial activity compared to PGCL. Fibronectin 1 (FN1), identified from single-cell transcriptome analysis, was loaded into the PGCL@1PAP/10CSPL scaffold to accelerate the abnormal NP degeneration.<i>In vitro</i>and<i>in vivo</i>experiments indicated that the PGCL@1PAP/10CSPL-FN1 scaffold enhanced osteogenic differentiation, promoted angiogenesis, and facilitated the removal of damaged disc tissue. This study introduces a novel implant system with desirable mechanical strength and unique bone-promoting and vascularizing properties for lumbar interbody fusion in IDD treatment.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":"17 1","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of immunocompetent full thickness skin tissue constructs to model skin fibrosis for high-throughput drug screening.","authors":"Yi Wei Lim, Russell Quinn, Kapil Bharti, Marc Ferrer, Hoda Zarkoob, Min Jae Song","doi":"10.1088/1758-5090/ad998c","DOIUrl":"10.1088/1758-5090/ad998c","url":null,"abstract":"<p><p>The lack of the immune component in most of the engineered skin models remains a challenge to study the interplay between different immune and non-immune cell types of the skin. Immunocompetent human<i>in vitro</i>skin models offer potential advantages in recapitulating<i>in vivo</i>like behavior which can serve to accelerate translational research and therapeutics development for skin diseases. Here we describe a three-dimensional human full-thickness skin (FTS) equivalent incorporating polarized M1 and M2 macrophages from human peripheral CD14⁺monocytes. This macrophage-incorporated FTS model demonstrates discernible immune responses with physiologically relevant cytokine production and macrophage plasticity under homeostatic and lipopolysaccharide stimulation conditions. M2-incorporated FTS recapitulates skin fibrosis phenotypes with transforming growth factor-<i>β</i>1 treatment as reflected by significant collagen deposition and myofibroblast expression, demonstrating a M2 potentiation effect. In conclusion, we successfully biofabricated an immunocompetent FTS with functional macrophages in a high-throughput (HT) amenable format. This model is the first step towards a HT-assay platform to develop new therapeutics for skin diseases.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11638742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing vaginal reconstruction through 3D bioprinted scaffolds using a novel vECM-GelMA-SF bioink.","authors":"Jiahua Zheng, Xuemei Zhang, Kaixuan Guo, Liman Yan, Xiaotong Xu, Wenxin Shi, Jingkun Zhang, Yanfang Du, Mingle Zhang, Xianghua Huang","doi":"10.1088/1758-5090/ad95bf","DOIUrl":"10.1088/1758-5090/ad95bf","url":null,"abstract":"<p><p>Overcoming the low cell survival rates and insufficient neovascularization associated with tissue engineering of the vagina is crucial for advancing the vaginal reconstruction. In this research, we have developed a unique bioink composed of porcine vaginal extracellular matrix (vECM), gelatin methacrylamide (GelMA), and silk fibroin (SF) to facilitate the bioprinting of a vaginal scaffold. The vECM-GelMA-SF bioink effectively replicates the<i>in vivo</i>microenvironment, supporting the<i>in vitro</i>cultivation of 3D bioprinted vaginal scaffolds. It promotes stem cell viability and enhances neovascularization by harnessing the mechanical properties of GelMA/SF and the tissue specificity of vECM.<i>In vivo</i>orthotopic studies have demonstrated that the use of 3D bioprinted vaginal scaffolds significantly improves the functionality of reconstructed vaginas, promoting angiogenesis, rapid epithelialization, muscle regeneration, glycogen secretion, and nerve repair. The reconstructed vaginal tissues in the 3D cell-loaded scaffold group closely resemble natural vaginal tissues. Differential proteomics analysis has provided insights into the genetic functions and biological pathways involved in vaginal reconstruction. Our study successfully optimized the composition of the vECM-GelMA-SF bioink, achieving a balance between biocompatibility and printability. This bioink is suitable for constructing 3D bioprinted vaginal scaffolds of various dimensions, transplantable<i>in situ</i>in animal models with different degrees of vaginal absence. The bioink may find applications in clinical settings, improving the overall effectiveness and safety of<i>in vivo</i>vaginal reconstruction procedures.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soft-lithographically defined template for arbitrarily patterned acoustic bioassembly.","authors":"Sihan Chen, Lili Zhu, Jibo Wang, Shanqing Jiang, Yuhang Fan, Wen Zhao, Zian Wang, Qing Zhou, Yun Chen, Pu Chen","doi":"10.1088/1758-5090/ad9637","DOIUrl":"10.1088/1758-5090/ad9637","url":null,"abstract":"<p><p>Acoustic bioassembly is recently regarded as a highly efficient biofabrication tool to generate functional tissue mimics. Despite their capacity of directly patterning live cells with close intercellular proximity, most acoustic bioassembly techniques are currently limited to generate some specific simple types of periodic and symmetric patterns, which represents an urgent challenge to emulate geometrically complex cytoarchitecture in human tissue. To address this challenge, we herein demonstrate a soft-lithographically defined acoustic bioassembly (SLAB) technique that enables to assemble live cells into geometrically defined arbitrary multicellular structures. Particularly, we employed a widely accessible soft lithography technique to fabricate a polydimethylsiloxane (PDMS) construct that works as an amplitude modulation template to define the pressure distribution of near-field acoustic waves. We found that zero pressure areas of the near-field acoustic waves at the PDMS surface distribute above the air-filling regions of the PDMS construct when both the PDMS top layer and air layer are approximately one-tenth of the acoustic wavelength. Using this technique, bioparticles can be assembled into symmetrical or asymmetrical patterns. Specifically, we have demonstrated the SLAB of endothelial spheroids and hepatic cells into liver tissue mimics (LTMs). The functional analysis further indicates that the formed LTMs displayed liver-specific functions, including albumin secretion, urea synthesis, glucose metabolism, and lipid storage. We expect this SLAB technique will be broadly used to construct complex functional tissues for tissue engineering and regenerative medicine.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A digital manufactured microfluidic platform for flexible construction of 3D co-culture tumor model with spatiotemporal resolution.","authors":"Chao Han, Renchao Zhang, Xiwen He, Yuan Fang, Gang Cen, Weidong Wu, Chen Huang, Xiang Chen","doi":"10.1088/1758-5090/ad9636","DOIUrl":"10.1088/1758-5090/ad9636","url":null,"abstract":"<p><p>The specific spatiotemporal distribution of diverse components in tumor microenvironment plays a crucial role in the cancer progression.<i>In vitro</i>three-dimensional (3D) tumor models with polydimethylsiloxane (PDMS) based microfluidic platform have been applied as useful tool to conduct studies from cancer biology to drug screening. However, PDMS has not been welcomed as a standardized commercial application for preclinical screening due to inherent limitations in scale-up production and molecule absorption. Here, we present a novel microfluidic platform to flexibly construct 3D co-culture models with spatiotemporal resolution by using multiple digital manufacturing technologies. The platform, which consist of reduplicative microfluidic chips, is made of biocompatible poly methyl methacrylate by fast laser cutting. Each replica includes a simple microfluidic chamber without internal structures which can be flexibly post-fabricated according to various research requirements. Digital light processing based 3D bioprinting was used to pattern fine hydrogel structures for post-fabrication on-chip. By multi-step bioprinting and automatic image alignment, we show that this approach provides sufficient design flexibility to construct 3D co-culture tumor model with spatiotemporal resolution to replicate microarchitecture of tumor microtissue<i>in situ</i>. And the tumor model has the potential to mimic tumor biology behaviors which can be used for mechanism study and drug test. Our microengineered tumor model may serve as an enabling tool to recapitulate pathophysiological complexity of tumor, and to systematically examine the contribution of the tumor microenvironment to the cancer progression. The proposed strategy can also be applied to help engineer diverse meaningful<i>in vitro</i>models for extensive biomedical applications, from physiology and disease study to therapy evaluation.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprinting a resilient and transparent cornea stroma equivalent: harnessing dual crosslinking strategy with decellularized cornea matrix and silk fibroin hybrid.","authors":"Anwesha Ghosh, Ashis Kumar Bera, Soham Ghosh, Vivek Singh, Sayan Basu, Falguni Pati","doi":"10.1088/1758-5090/ad9409","DOIUrl":"10.1088/1758-5090/ad9409","url":null,"abstract":"<p><p>Bioprinting a resilient yet optically transparent corneal tissue substitute remains a challenge. In this study we introduce an innovative methodology aimed at bolstering the mechanical and optical attributes of silk fibroin (SF) hydrogels, pivotal for the progression of cornea tissue engineering. We devised a unique eosin Y-based photoinitiator system to instigate di-tyrosine linkages within highly concentrated pristine SF solutions under green light exposure. This pioneering technique resulted in SF hydrogels fortified by dityrosine covalent bonds, preserving exceptional transparency and soft elastomeric qualities devoid of spontaneous transitions to stiff, opaque beta-sheet conformations. Furthermore, we synergistically combined SF with decellularized cornea matrix (DCM) hydrogel, leveraging photo-polymerization under green light followed by thermal gelation to establish resilient and stable gel formation. The ensuing dual crosslinked hybrid hydrogels exhibited superior mechanical and thermal resilience in comparison to dual crosslinked DCM hydrogels. The inclusion of SF in DCM further augmented the hydrogel's elasticity and shear recovery, positioning it as an optimal bioink for cornea bioprinting endeavors. During the extrusion printing process, photocrosslinking of the bioink superficially fortified SF and DCM polymer chains via di-tyrosine linkages, furnishing initial stability and mechanical fortitude. Subsequent post-printing thermal gelation further reinforced collagen chains through self-assembly. Notably, the bioprinted cornea constructs, housing human limbal mesenchymal stem cells, manifested transparency, structural integrity, and optimal functionality, underscored by the expression of keratocyte proteoglycans. In summation, our engineered 3D constructs exhibit promising potential for<i>in vivo</i>applications in cornea tissue engineering, marking a significant stride forward in the field's advancement.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel<i>in situ</i>and rapid self-gelation recombinant collagen-like protein hydrogel for wound regeneration: mediated by metal coordination crosslinking and reinforced by electro-oxidized tea polyphenols.","authors":"Yue Sun, Cungang Gao, Pengxin Jia, Liang Song, Jia Kang, Min Han, Wenfa Yu, Rui Nian","doi":"10.1088/1758-5090/ad9408","DOIUrl":"10.1088/1758-5090/ad9408","url":null,"abstract":"<p><p>Recombinant collagen holds immense potential in the development of medical functional materials, yet its widespread application remains hindered by the absence of a suitable self-assembly strategy. In this article, we report the discovery that the bacterial-derived collagen-like (CL) protein Scl2 can rapidly self-gelation (∼1 min at pH ∼7) due to properties enabled by metal coordination crosslinking. This was achieved by fusing metal ion chelating peptides to both termini of the protein. Our research further reveals the critical role of electrostatic interaction between globular domains (V domains) of recombinant collagen in the self-assembly process. We show that modifying the negative charge load of the N-terminal<i>α</i>-helix of the V domain enables control over the self-assembly time (from 1 min to 30 min) and strength (from 8 kPa to 26 kPa) of the Scl2 hydrogel. By adjusting the molecular weight of the core CL domain, we have remarkably further enhanced the strength of the Scl2 hydrogel to 78 kPa. Moreover, we innovatively employed electro-oxidized tea polyphenols to enhance the stability of the Scl2 hydrogel, resulting in the formation of a reliable self-assembled metal coordination hydrogel at physiological temperature. This approach not only eliminates the need for toxic chemical crosslinking agents but also confers the material with multiple functionalities, such as adhesion, antibacterial, and antioxidant properties. The novel recombinant Scl2 hydrogel exhibited exceptional<i>in situ</i>self-gelation and injectable properties. This innovative hydrogel not only demonstrates remarkable biological activity but also exhibits remarkable tissue repair-promoting capabilities in full-thickness skin injury models (shorten healing cycle by more than 30%). The convenient and versatile nature of this recombinant collagen hydrogel makes it promising for clinical applications in injury treatment, demonstrating broad applications in the future.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-biofertilizers: utilizing nanopolymers as coating matrix-a comprehensive review.","authors":"Navin Kumar Thirumurugan, Gomathi Velu, Senthilkumar Murugaiyan, Djanaguiraman Maduraimuthu, Sathyamoorthy Ponnuraj, Sharmila D J, K S Subramanian","doi":"10.1088/1758-5090/ad94a8","DOIUrl":"10.1088/1758-5090/ad94a8","url":null,"abstract":"<p><p>In modern agriculture, nanotechnology was recognized as a potentially transformative innovation. Nanopolymers as coating matrix in nano-biofertilizer has a massive impact on agricultural productivity. The integration of nanotechnology with biofertilizers has led to the creation of nano-biofertilizer formulations that enhance nutrient delivery, improve plant growth, and increase resistance to environmental stress. Nanopolymers, both synthetic and biogenic, including chitosan, cellulose, gelatin, sodium alginate, starch, and polyvinyl alcohol, are utilized as encapsulating materials. They are effective in ensuring controlled nutrient release and shielding beneficial microorganisms from external environmental conditions. Studies indicate that nano-biofertilizers improve soil quality, raise crop yields, and reduce the usage of chemical fertilizers to enhance sustainable agricultural practices. The review also addresses the microbial encapsulation methodology, release kinetics, phytotoxicity, challenges and future prospects of nano-biofertilizer technology, including nanoparticle-bacteria interaction, scalability, and regulatory considerations. This paper elaborates the potential and limitations of nano-biofertilizers, providing insights for future advancements in the agriculture field.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}