Biofabrication最新文献_第9页

Histo-pillar strip for optimal histogel block construction and biomarker analysis in 3D-lung cancer patient-derived organoids. 在三维肺癌患者衍生器官组织中优化组织凝胶块构建和生物标记分析的组织柱条。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-08-07 DOI: 10.1088/1758-5090/ad68a7

Sang-Yun Lee, Eunyoung Lee, Ji-O Ryu, Kyuhwan Kim, Yongki Hwang, Bosung Ku, Seok Whan Moon, Mi Hyoung Moon, Kyung Soo Kim, Kwanyong Hyun, Jeong Uk Lim, Chan Kwon Park, Sung Won Kim, Chang Dong Yeo, Dong Woo Lee, Seung Joon Kim

{"title":"Histo-pillar strip for optimal histogel block construction and biomarker analysis in 3D-lung cancer patient-derived organoids.","authors":"Sang-Yun Lee, Eunyoung Lee, Ji-O Ryu, Kyuhwan Kim, Yongki Hwang, Bosung Ku, Seok Whan Moon, Mi Hyoung Moon, Kyung Soo Kim, Kwanyong Hyun, Jeong Uk Lim, Chan Kwon Park, Sung Won Kim, Chang Dong Yeo, Dong Woo Lee, Seung Joon Kim","doi":"10.1088/1758-5090/ad68a7","DOIUrl":"10.1088/1758-5090/ad68a7","url":null,"abstract":"This study proposed an optimized histogel construction method for histological analysis by applying lung cancer patient-derived organoids (PDOs) to the developed histo-pillar strip. Previously, there is the cultured PDOs damage problem during the histogel construction due to forced detachment of the Matrigel spots from the 96-well plate bottom. To address this issue, we cultured PDO on the proposed Histo-pillar strips and then immersed them in 4% paraformaldehyde fixation solution to self-isolate PDO without damage. The 4μl patient-derived cell (PDC)/Matrigel mixtures were dispensed on the surface of a U-shaped histo-pillar strip, and the PDCs were aggregated by gravity and cultured into PDOs. Cultured PDOs were self-detached by simply immersing them in a paraformaldehyde fixing solution without physical processing, showing about two times higher cell recovery rate than conventional method. In addition, we proposed a method for embedding PDOs under conditions where the histogel temperature was maintained such that the histogel did not harden, thereby improving the problem of damaging the histogel block in the conventional sandwich histogel construction method. We performed histological and genotyping analyses using tumor tissues and PDOs from two patients with lung adenocarcinoma. Therefore, the PDO culture and improved histogel block construction method using the histo-pillar strip proposed in this study can be employed as useful tools for the histological analysis of a limited number of PDCs.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141791829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofabricated nanomaterials in sustainable agriculture: insights, challenges and prospects. 可持续农业中的生物纳米材料：见解、挑战和前景。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-08-05 DOI: 10.1088/1758-5090/ad60f7

Pratikhya Mohanty, Puneet Kumar Singh, Basundhara Lenka, Tapan K Adhya, Suresh K Verma, Zobia Ayreen, Shilpita Patro, Biplab Sarkar, Ranjan K Mohapatra, Snehasish Mishra

{"title":"Biofabricated nanomaterials in sustainable agriculture: insights, challenges and prospects.","authors":"Pratikhya Mohanty, Puneet Kumar Singh, Basundhara Lenka, Tapan K Adhya, Suresh K Verma, Zobia Ayreen, Shilpita Patro, Biplab Sarkar, Ranjan K Mohapatra, Snehasish Mishra","doi":"10.1088/1758-5090/ad60f7","DOIUrl":"10.1088/1758-5090/ad60f7","url":null,"abstract":"One ever-evolving and ever-demanding critical human endeavour is the provision of food security for the growing world population. This can be done by adopting sustainable agriculture through horizontal (expanding the arable land area) and vertical (intensifying agriculture through sound technological approaches) interventions. Customized formulated nanomaterials have numerous advantages. With their specialized physico-chemical properties, some nanoparticulated materials improve the plant's natural development and stress tolerance and some others are good nanocarriers. Nanocarriers in agriculture often coat chemicals to form composites having utilities with crop productivity enhancement abilities, environmental management (such as ecotoxicity reduction ability) and biomedicines (such as the ability to control and target the release of useful nanoscale drugs). Ag, Fe, Zn, TiO2, ZnO, SiO2and MgO nanoparticles (NPs), often employed in advanced agriculture, are covered here. Some NPs used for various extended purposes in modern farming practices, including disease diagnostics and seed treatment are also covered. Thus, nanotechnology has revolutionized agrotechnology, which holds promise to transform agricultural (ecosystems as a whole to ensure food security in the future. Considering the available literature, this article further probes the emergent regulatory issues governing the synthesis and use of nanomaterials in the agriculture sector. If applied responsibly, nanomaterials could help improve soil health. This article provides an overview of the nanomaterials used in the distribution of biomolecules, to aid in devising a safer and eco-friendly sustainable agriculture strategy. Through this, agri-systems that depend on advanced farming practices might function more effectively and enhance agri-productivity to meet the food demand of the rising world population.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141562541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Robust and customizable spheroid culture system for regenerative medicine. 用于再生医学的稳健且可定制的球形培养系统。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-08-02 DOI: 10.1088/1758-5090/ad6795

Kyoung Hwan Park, Thuy Trang Truong, Jae-Hyun Park, Yujin Park, Hyeok Kim, Sung-Ae Hyun, Hye-Eun Shim, Sudipta Mallick, Hun-Jun Park, Kang Moo Huh, Sun-Woong Kang

引用次数: 0

Efficacy of 3D printed anatomically equivalent thermoplastic polyurethane guide conduits in promoting the regeneration of critical-sized peripheral nerve defects. 三维打印的解剖学等效热塑性聚氨酯导管在促进临界大小周围神经缺损再生方面的功效。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-07-25 DOI: 10.1088/1758-5090/ad5fbe

Allen Zennifer, David Raj Chellappan, Prabu Chinnaswamy, Anuradha Subramanian, Dhakshinamoorthy Sundaramurthi, Swaminathan Sethuraman

{"title":"Efficacy of 3D printed anatomically equivalent thermoplastic polyurethane guide conduits in promoting the regeneration of critical-sized peripheral nerve defects.","authors":"Allen Zennifer, David Raj Chellappan, Prabu Chinnaswamy, Anuradha Subramanian, Dhakshinamoorthy Sundaramurthi, Swaminathan Sethuraman","doi":"10.1088/1758-5090/ad5fbe","DOIUrl":"10.1088/1758-5090/ad5fbe","url":null,"abstract":"Three-dimensional (3D) printing is an emerging tool for creating patient-specific tissue constructs analogous to the native tissue microarchitecture. In this study, anatomically equivalent 3D nerve conduits were developed using thermoplastic polyurethane (TPU) by combining reverse engineering and material extrusion (i.e. fused deposition modeling) technique. Printing parameters were optimized to fabricate nerve-equivalent TPU constructs. The TPU constructs printed with different infill densities supported the adhesion, proliferation, and gene expression of neuronal cells. Subcutaneous implantation of the TPU constructs for three months in rats showed neovascularization with negligible local tissue inflammatory reactions and was classified as a non-irritant biomaterial as per ISO 10993-6. To performin vivoefficacy studies, nerve conduits equivalent to rat's sciatic nerve were fabricated and bridged in a 10 mm sciatic nerve transection model. After four months of implantation, the sensorimotor function and histological assessments revealed that the 3D printed TPU conduits promoted the regeneration in critical-sized peripheral nerve defects equivalent to autografts. This study proved that TPU-based 3D printed nerve guidance conduits can be created to replicate the complicated features of natural nerves that can promote the regeneration of peripheral nerve defects and also show the potential to be extended to several other tissues for regenerative medicine applications.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141537482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A practical machine learning approach for predicting the quality of 3D (bio)printed scaffolds. 预测三维（生物）打印支架质量的实用机器学习方法。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-07-25 DOI: 10.1088/1758-5090/ad6374

Saeed Rafieyan, Elham Ansari, Ebrahim Vasheghani-Farahani

{"title":"A practical machine learning approach for predicting the quality of 3D (bio)printed scaffolds.","authors":"Saeed Rafieyan, Elham Ansari, Ebrahim Vasheghani-Farahani","doi":"10.1088/1758-5090/ad6374","DOIUrl":"10.1088/1758-5090/ad6374","url":null,"abstract":"3D (Bio)printing is a highly effective method for fabricating tissue engineering scaffolds, renowned for their exceptional precision and control. Artificial intelligence (AI) has become a crucial technology in this field, capable of learning and replicating complex patterns that surpass human capabilities. However, the integration of AI in tissue engineering is often hampered by the lack of comprehensive and reliable data. This study addresses these challenges by providing one of the most extensive datasets on 3D-printed scaffolds. It provides the most comprehensive open-source dataset and employs various AI techniques, from unsupervised to supervised learning. This dataset includes detailed information on 1171 scaffolds, featuring a variety of biomaterials and concentrations-including 60 biomaterials such as natural and synthesized biomaterials, crosslinkers, enzymes, etc.-along with 49 cell lines, cell densities, and different printing conditions. We used over 40 machine learning and deep learning algorithms, tuning their hyperparameters to reveal hidden patterns and predict cell response, printability, and scaffold quality. The clustering analysis using KMeans identified five distinct ones. In classification tasks, algorithms such as XGBoost, Gradient Boosting, Extra Trees Classifier, Random Forest Classifier, and LightGBM demonstrated superior performance, achieving higher accuracy and F1 scores. A fully connected neural network with six hidden layers from scratch was developed, precisely tuning its hyperparameters for accurate predictions. The developed dataset and the associated code are publicly available onhttps://github.com/saeedrafieyan/MLATEto promote future research.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of heterocellular spheroids with controllable core-shell structure using inertial focusing effect for scaffold-free 3D cell culture models. 利用惯性聚焦效应制作具有可控核壳结构的异细胞球体，用于无支架三维细胞培养模型。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-07-25 DOI: 10.1088/1758-5090/ad647e

Tan Tang, Pengfei Zhang, Qiuting Zhang, Xingkun Man, Ye Xu

{"title":"Fabrication of heterocellular spheroids with controllable core-shell structure using inertial focusing effect for scaffold-free 3D cell culture models.","authors":"Tan Tang, Pengfei Zhang, Qiuting Zhang, Xingkun Man, Ye Xu","doi":"10.1088/1758-5090/ad647e","DOIUrl":"10.1088/1758-5090/ad647e","url":null,"abstract":"Three-dimensional (3D) cell culture models capable of emulating the biological functions of natural tissues are pivotal in tissue engineering and regenerative medicine. Despite progress, the fabrication ofin vitroheterocellular models that mimic the intricate structures of natural tissues remains a significant challenge. In this study, we introduce a novel, scaffold-free approach leveraging the inertial focusing effect in rotating hanging droplets for the reliable production of heterocellular spheroids with controllable core-shell structures. Our method offers precise control over the core-shell spheroid's size and geometry by adjusting the cell suspension density and droplet morphology. We successfully applied this technique to create hair follicle organoids, integrating dermal papilla cells within the core and epidermal cells in the shell, thereby achieving markedly enhanced hair inducibility compared to mixed-structure models. Furthermore, we have developed melanoma tumor spheroids that accurately mimic the dynamic interactions between tumor and stromal cells, showing increased invasion capabilities and altered expressions of cellular adhesion molecules and proteolytic enzymes. These findings underscore the critical role of cellular spatial organization in replicating tissue functionalityin vitro. Our method represents a significant advancement towards generating heterocellular spheroids with well-defined architectures, offering broad implications for biological research and applications in tissue engineering.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct 3D printing of freeform anisotropic bioactive structure based on shear-oriented ink system. 基于剪切导向油墨系统的自由形态各向异性生物活性结构的直接三维打印。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-07-24 DOI: 10.1088/1758-5090/ad6375

Chenhui Yuan, Jinhong Jiang, Xinyu Zhang, Lin Gu, Xueping Wang, Lei Shao

引用次数: 0

Beyond stiffness: deciphering the role of viscoelasticity in cancer evolution and treatment response. 超越僵化：解读粘弹性在癌症演变和治疗反应中的作用。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-07-24 DOI: 10.1088/1758-5090/ad5705

Ana Zubiarrain-Laserna, Daniel Martínez-Moreno, Julia López de Andrés, Laura de Lara-Peña, Olatz Guaresti, Ane Miren Zaldua, Gema Jiménez, Juan Antonio Marchal

{"title":"Beyond stiffness: deciphering the role of viscoelasticity in cancer evolution and treatment response.","authors":"Ana Zubiarrain-Laserna, Daniel Martínez-Moreno, Julia López de Andrés, Laura de Lara-Peña, Olatz Guaresti, Ane Miren Zaldua, Gema Jiménez, Juan Antonio Marchal","doi":"10.1088/1758-5090/ad5705","DOIUrl":"10.1088/1758-5090/ad5705","url":null,"abstract":"There is increasing evidence that cancer progression is linked to tissue viscoelasticity, which challenges the commonly accepted notion that stiffness is the main mechanical hallmark of cancer. However, this new insight has not reached widespread clinical use, as most clinical trials focus on the application of tissue elasticity and stiffness in diagnostic, therapeutic, and surgical planning. Therefore, there is a need to advance the fundamental understanding of the effect of viscoelasticity on cancer progression, to develop novel mechanical biomarkers of clinical significance. Tissue viscoelasticity is largely determined by the extracellular matrix (ECM), which can be simulatedin vitrousing hydrogel-based platforms. Since the mechanical properties of hydrogels can be easily adjusted by changing parameters such as molecular weight and crosslinking type, they provide a platform to systematically study the relationship between ECM viscoelasticity and cancer progression. This review begins with an overview of cancer viscoelasticity, describing how tumor cells interact with biophysical signals in their environment, how they contribute to tumor viscoelasticity, and how this translates into cancer progression. Next, an overview of clinical trials focused on measuring biomechanical properties of tumors is presented, highlighting the biomechanical properties utilized for cancer diagnosis and monitoring. Finally, this review examines the use of biofabricated tumor models for studying the impact of ECM viscoelasticity on cancer behavior and progression and it explores potential avenues for future research on the production of more sophisticated and biomimetic tumor models, as well as their mechanical evaluation.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Riboflavin overproduction from diverse feedstocks with engineeredCorynebacterium glutamicum. 利用工程化谷氨酸棒杆菌从不同原料中超量生产核黄素。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-07-24 DOI: 10.1088/1758-5090/ad628e

Fernando Pérez-García, Luciana Fernandes Brito, Thea Isabel Bakken, Trygve Brautaset

{"title":"Riboflavin overproduction from diverse feedstocks with engineeredCorynebacterium glutamicum.","authors":"Fernando Pérez-García, Luciana Fernandes Brito, Thea Isabel Bakken, Trygve Brautaset","doi":"10.1088/1758-5090/ad628e","DOIUrl":"10.1088/1758-5090/ad628e","url":null,"abstract":"Riboflavin overproduction byCorynebacterium glutamicumwas achieved by screening synthetic operons, enabling fine-tuned expression of the riboflavin biosynthetic genesribGCAH.The synthetic operons were designed by means of predicted translational initiation rates of each open reading frame, with the best-performing selection enabling riboflavin overproduction without negatively affecting cell growth. Overexpression of the fructose-1,6-bisphosphatase (fbp) and 5-phosphoribosyl 1-pyrophosphate aminotransferase (purF) encoding genes was then done to redirect the metabolic flux towards the riboflavin precursors. The resulting strain produced 8.3 g l-1of riboflavin in glucose-based fed-batch fermentations, which is the highest reported riboflavin titer withC. glutamicum. Further genetic engineering enabled both xylose and mannitol utilization byC. glutamicum, and we demonstrated riboflavin overproduction with the xylose-rich feedstocks rice husk hydrolysate and spent sulfite liquor, and the mannitol-rich feedstock brown seaweed hydrolysate. Remarkably, rice husk hydrolysate provided 30% higher riboflavin yields compared to glucose in the bioreactors.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141598280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing neovascularization post-myocardial infarction through injectable hydrogel functionalized with endothelial-derived EVs. 通过注射内皮细胞衍生的 EVs 功能化水凝胶，增强心肌梗塞后的新生血管。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-07-23 DOI: 10.1088/1758-5090/ad6190

Fabio Maiullari, Marika Milan, Maila Chirivì, Maria Grazia Ceraolo, Salma Bousselmi, Nicole Fratini, Matteo Galbiati, Orazio Fortunato, Marco Costantini, Francesca Brambilla, Pierluigi Mauri, Dario Di Silvestre, Antonella Calogero, Tommaso Sciarra, Roberto Rizzi, Claudia Bearzi

{"title":"Enhancing neovascularization post-myocardial infarction through injectable hydrogel functionalized with endothelial-derived EVs.","authors":"Fabio Maiullari, Marika Milan, Maila Chirivì, Maria Grazia Ceraolo, Salma Bousselmi, Nicole Fratini, Matteo Galbiati, Orazio Fortunato, Marco Costantini, Francesca Brambilla, Pierluigi Mauri, Dario Di Silvestre, Antonella Calogero, Tommaso Sciarra, Roberto Rizzi, Claudia Bearzi","doi":"10.1088/1758-5090/ad6190","DOIUrl":"10.1088/1758-5090/ad6190","url":null,"abstract":"Over the past three decades, cell therapy development has fallen short of expectations, with many cellular sources demonstrating a 'Janus effect' and raising safety concerns. Extracellular vesicles (EVs), supported by advanced technologies, present a promising avenue in regenerative medicine, offering benefits such as immune tolerance and avoidance of negative aspects associated with cell transplants. Our previous research showcased enhanced and organized subcutaneous vascularization using three-dimensional bioprinted patches containing HUVEC-derived EVs in immunodeficient animal models. In this context, stress conditions on the cells of origin further boosted the EVs' neoangiogenic potential. Since neovascularization is the first regenerative target requiring restoration, the present study aims to complement our previous work by employing an injectable gelatin methacrylate (GelMA) hydrogel functionalized with HUVEC-derived EVs in a pathological condition of acute myocardial infarction. This bioactive hydrogel resulted in reduced fibrosis, improved contractility, and promoted angiogenesis, showing promise in countering tissue deterioration and addressing vascular deficits. Moreover, the molecular characterization of EVs through miRNome and proteomic analyses further supports their potential as bio-additives for hydrogel functionalization. This cell-free approach mitigates immune rejection and oncogenic risks, offering innovative therapeutic advantages.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141578939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0