Biofabrication最新文献_第9页

A perfusable, vascularized kidney organoid-on-chip model. 可灌注、血管化的肾脏类器官芯片模型。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-07-05 DOI: 10.1088/1758-5090/ad5ac0

Katharina T Kroll, Kimberly A Homan, Sebastien G M Uzel, Mariana M Mata, Kayla J Wolf, Jonathan E Rubins, Jennifer A Lewis

引用次数: 0

Development of an organ-on-chip model for the detection of volatile organic compounds as potential biomarkers of tumour progression. 开发用于检测挥发性有机化合物的芯片器官模型，作为肿瘤进展的潜在生物标记物。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-07-04 DOI: 10.1088/1758-5090/ad5764

Clara Bayona, Magdalena Wrona, Teodora Ranđelović, Cristina Nerín, Jesús Salafranca, Ignacio Ochoa

引用次数: 0

3D embedded bioprinting of large-scale intestine with complex structural organization and blood capillaries. 具有复杂结构组织和毛细血管的大规模肠道三维嵌入式生物打印。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-07-03 DOI: 10.1088/1758-5090/ad5b1b

Yuxuan Li, Shengnan Cheng, Haihua Shi, Renshun Yuan, Chen Gao, Yuhan Wang, Zhijun Zhang, Zongwu Deng, Jie Huang

{"title":"3D embedded bioprinting of large-scale intestine with complex structural organization and blood capillaries.","authors":"Yuxuan Li, Shengnan Cheng, Haihua Shi, Renshun Yuan, Chen Gao, Yuhan Wang, Zhijun Zhang, Zongwu Deng, Jie Huang","doi":"10.1088/1758-5090/ad5b1b","DOIUrl":"10.1088/1758-5090/ad5b1b","url":null,"abstract":"Accurate reproduction of human intestinal structure and functionin vitrois of great significance for understanding the development and disease occurrence of the gut. However, mostin vitrostudies are often confined to 2D models, 2.5D organ chips or 3D organoids, which cannot fully recapitulate the tissue architecture, microenvironment and cell compartmentalization foundin vivo. Herein, a centimeter-scale intestine tissue that contains intestinal features, such as hollow tubular structure, capillaries and tightly connected epithelium with invivo-likering folds, crypt-villi, and microvilli is constructed by 3D embedding bioprinting. In our strategy, a novel photocurable bioink composed of methacrylated gelatin, methacrylated sodium alginate and poly (ethylene glycol) diacrylate is developed for the fabrication of intestinal model. The Caco-2 cells implanted in the lumen are induced by the topological structures of the model to derive microvilli, crypt-villi, and tight junctions, simulating the intestinal epithelial barrier. The human umbilical vein endothelial cells encapsulated within the model gradually form microvessels, mimicking the dense capillary network in the intestine. This intestine-like tissue, which closely resembles the structure and cell arrangement of the human gut, can act as a platform to predict the therapeutic and toxic side effects of new drugs on the intestine.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141445360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human iPSC-derived liver co-culture spheroids to model liver fibrosis. 人类 iPSC 衍生肝脏共培养球体用于模拟肝纤维化。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-06-28 DOI: 10.1088/1758-5090/ad5766

Laura Cools, Mina Kazemzadeh Dastjerd, Ayla Smout, Vincent Merens, Yuwei Yang, Hendrik Reynaert, Nouredin Messaoudi, Vincent De Smet, Manoj Kumar, Stefaan Verhulst, Catherine Verfaillie, Leo A van Grunsven

{"title":"Human iPSC-derived liver co-culture spheroids to model liver fibrosis.","authors":"Laura Cools, Mina Kazemzadeh Dastjerd, Ayla Smout, Vincent Merens, Yuwei Yang, Hendrik Reynaert, Nouredin Messaoudi, Vincent De Smet, Manoj Kumar, Stefaan Verhulst, Catherine Verfaillie, Leo A van Grunsven","doi":"10.1088/1758-5090/ad5766","DOIUrl":"10.1088/1758-5090/ad5766","url":null,"abstract":"The lack of adequate humanin vitromodels that recapitulate the cellular composition and response of the human liver to injury hampers the development of anti-fibrotic drugs. The goal of this study was to develop a human spheroid culture model to study liver fibrosis by using induced pluripotent stem cell (iPSC)-derived liver cells. iPSCs were independently differentiated towards hepatoblasts (iHepatoblasts), hepatic stellate cells (iHSCs), endothelial cells (iECs) and macrophages (iMΦ), before assembly into free floating spheroids by culturing cells in 96-well U-bottom plates and orbital shaking for up to 21 days to allow further maturation. Through transcriptome analysis, we show further maturation of iECs and iMΦ, the differentiation of the iHepatoblasts towards hepatocyte-like cells (iHeps) and the inactivation of the iHSCs by the end of the 3D culture. Moreover, these cultures display a similar expression of cell-specific marker genes (CYP3A4, PDGFRβ, CD31andCD68) and sensitivity to hepatotoxicity as spheroids made using freshly isolated primary human liver cells. Furthermore, we show the functionality of the iHeps and the iHSCs by mimicking liver fibrosis through iHep-induced iHSC activation, using acetaminophen. In conclusion, we have established a reproducible human iPSC-derived liver culture model that can be used to mimic fibrosisin vitroas a replacement of primary human liver derived 3D models. The model can be used to investigate pathways involved in fibrosis development and to identify new targets for chronic liver disease therapy.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141309899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cancer cell migration depends on adjacent ASC and adipose spheroids in a 3D bioprinted breast cancer model. 在三维生物打印乳腺癌模型中，癌细胞迁移取决于相邻的 ASC 和脂肪球。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-06-27 DOI: 10.1088/1758-5090/ad57f7

Hannes Horder, David Böhringer, Nadine Endrizzi, Laura S Hildebrand, Alessandro Cianciosi, Sabrina Stecher, Franziska Dusi, Sophie Schweinitzer, Martin Watzling, Jürgen Groll, Tomasz Jüngst, Jörg Teßmar, Petra Bauer-Kreisel, Ben Fabry, Torsten Blunk

{"title":"Cancer cell migration depends on adjacent ASC and adipose spheroids in a 3D bioprinted breast cancer model.","authors":"Hannes Horder, David Böhringer, Nadine Endrizzi, Laura S Hildebrand, Alessandro Cianciosi, Sabrina Stecher, Franziska Dusi, Sophie Schweinitzer, Martin Watzling, Jürgen Groll, Tomasz Jüngst, Jörg Teßmar, Petra Bauer-Kreisel, Ben Fabry, Torsten Blunk","doi":"10.1088/1758-5090/ad57f7","DOIUrl":"https://doi.org/10.1088/1758-5090/ad57f7","url":null,"abstract":"Breast cancer develops in close proximity to mammary adipose tissue and interactions with the local adipose environment have been shown to drive tumor progression. The specific role, however, of this complex tumor microenvironment in cancer cell migration still needs to be elucidated. Therefore, in this study, a 3D bioprinted breast cancer model was developed that allows for a comprehensive analysis of individual tumor cell migration parameters in dependence of adjacent adipose stroma. In this co-culture model, a breast cancer compartment with MDA-MB-231 breast cancer cells embedded in collagen is surrounded by an adipose tissue compartment consisting of adipose-derived stromal cell (ASC) or adipose spheroids in a printable bioink based on thiolated hyaluronic acid. Printing parameters were optimized for adipose spheroids to ensure viability and integrity of the fragile lipid-laden cells. Preservation of the adipogenic phenotype after printing was demonstrated by quantification of lipid content, expression of adipogenic marker genes, the presence of a coherent adipo-specific extracellular matrix, and cytokine secretion. The migration of tumor cells as a function of paracrine signaling of the surrounding adipose compartment was then analyzed using live-cell imaging. The presence of ASC or adipose spheroids substantially increased key migration parameters of MDA-MB-231 cells, namely motile fraction, persistence, invasion distance, and speed. These findings shed new light on the role of adipose tissue in cancer cell migration. They highlight the potential of our 3D printed breast cancer-stroma model to elucidate mechanisms of stroma-induced cancer cell migration and to serve as a screening platform for novel anti-cancer drugs targeting cancer cell dissemination.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":"16 3","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141455134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment and process optimization of high throughput biofabrication of immunocompetent breast cancer model for drug screening applications. 评估和优化用于药物筛选的免疫功能性乳腺癌模型的高通量生物制造工艺。

IF 8.2 2区医学

Biofabrication Pub Date : 2024-06-27 DOI: 10.1088/1758-5090/ad586b

Priyanshu Shukla, Ashis Kumar Bera, Amit Ghosh, Gaddam Kiranmai, Falguni Pati

{"title":"Assessment and process optimization of high throughput biofabrication of immunocompetent breast cancer model for drug screening applications.","authors":"Priyanshu Shukla, Ashis Kumar Bera, Amit Ghosh, Gaddam Kiranmai, Falguni Pati","doi":"10.1088/1758-5090/ad586b","DOIUrl":"10.1088/1758-5090/ad586b","url":null,"abstract":"Recent advancements in 3D cancer modeling have significantly enhanced our ability to delve into the intricacies of carcinogenesis. Despite the pharmaceutical industry's substantial investment of both capital and time in the drug screening and development pipeline, a concerning trend persists: drug candidates screened on conventional cancer models exhibit a dismal success rate in clinical trials. One pivotal factor contributing to this discrepancy is the absence of drug testing on pathophysiologically biomimetic 3D cancer models during pre-clinical stages. Unfortunately, current manual methods of 3D cancer modeling, such as spheroids and organoids, suffer from limitations in reproducibility and scalability. In our study, we have meticulously developed 3D bioprinted breast cancer model utilizing decellularized adipose tissue-based hydrogel obtained via a detergent-free decellularization method. Our innovative printing techniques allows for rapid, high-throughput fabrication of 3D cancer models in a 96-well plate format, demonstrating unmatched scalability and reproducibility. Moreover, we have conducted extensive validation, showcasing the efficacy of our platform through drug screening assays involving two potent anti-cancer drugs, 5-Fluorouracil and PRIMA-1Met. Notably, our platform facilitates effortless imaging and gene expression analysis, streamlining the evaluation process. In a bid to enhance the relevance of our cancer model, we have introduced a heterogeneous cell population into the DAT-based bioink. Through meticulous optimization and characterization, we have successfully developed a biomimetic immunocompetent breast cancer model, complete with microenvironmental cues and diverse cell populations. This breakthrough paves the way for rapid multiplex drug screening and the development of personalized cancer models, marking a paradigm shift in cancer research and pharmaceutical development.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141320479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of 3D engineered intestinal tissue producing abundant mucus by air-liquid interface culture using paper-based dual-layer scaffold. 利用纸基双层支架，通过气液界面培养法制造可产生大量粘液的三维工程肠组织。

IF 9 2区医学

Biofabrication Pub Date : 2024-06-17 DOI: 10.1088/1758-5090/ad504b

Mari Nagasawa, Mai Onuki, Natsuki Imoto, Kazuomi Tanaka, Ryo Tanaka, Moeka Kawada, Keiichi Imato, Kenta Iitani, Yuji Tsuchido, Naoya Takeda

{"title":"Fabrication of 3D engineered intestinal tissue producing abundant mucus by air-liquid interface culture using paper-based dual-layer scaffold.","authors":"Mari Nagasawa, Mai Onuki, Natsuki Imoto, Kazuomi Tanaka, Ryo Tanaka, Moeka Kawada, Keiichi Imato, Kenta Iitani, Yuji Tsuchido, Naoya Takeda","doi":"10.1088/1758-5090/ad504b","DOIUrl":"10.1088/1758-5090/ad504b","url":null,"abstract":"Fabrication of engineered intestinal tissues with the structures and functions as humans is crucial and promising as the tools for developing drugs and functional foods. The aim of this study is to fabricate an engineered intestinal tissue from Caco-2 cells by air-liquid interface culture using a paper-based dual-layer scaffold and analyze its structure and functions. Just by simply placing on a folded paper soaked in the medium, the electrospun gelatin microfiber mesh as the upper cell adhesion layer of the dual-layer scaffold was exposed to the air, while the lower paper layer worked to preserve and supply the cell culture medium to achieve stable culture over several weeks. Unlike the flat tissue produced using the conventional commercial cultureware, Transwell, the engineered intestinal tissue fabricated in this study formed three-dimensional villous architectures. Microvilli and tight junction structures characteristic of epithelial tissue were also formed at the apical side. Furthermore, compared to the tissue prepared by Transwell, mucus production was significantly larger, and the enzymatic activities of drug metabolism and digestion were almost equivalent. In conclusion, the air-liquid interface culture using the paper-based dual-layer scaffold developed in this study was simple but effective in fabricating the engineered intestinal tissue with superior structures and functions.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":9.0,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141093012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a biofabricated 3Din vitrovessel model for investigating transendothelial migration in stem cell therapy. 开发生物制造的三维体外血管模型，用于研究干细胞疗法中的跨内皮迁移。

IF 9 2区医学

Biofabrication Pub Date : 2024-06-13 DOI: 10.1088/1758-5090/ad51a5

Mattis Wachendörfer, Alena Lisa Palkowitz, Horst Fischer

{"title":"Development of a biofabricated 3Din vitrovessel model for investigating transendothelial migration in stem cell therapy.","authors":"Mattis Wachendörfer, Alena Lisa Palkowitz, Horst Fischer","doi":"10.1088/1758-5090/ad51a5","DOIUrl":"10.1088/1758-5090/ad51a5","url":null,"abstract":"Systemic stem cell therapies hold promise for treating severe diseases, but their efficiency is hampered by limited migration of injected stem cells across vascular endothelium towards diseased tissues. Understanding transendothelial migration is crucial for improving therapy outcomes. We propose a novel 3Din vitrovessel model that aids to unravel these mechanisms and thereby facilitates stem cell therapy development. Our model simulates inflammation through cytokine diffusion from the tissue site into the vessel. It consists of a biofabricated vessel embedded in a fibrin hydrogel, mimicking arterial wall composition with smooth muscle cells and fibroblasts. The perfusable channel is lined with a functional endothelium which expresses vascular endothelial cadherin, provides an active barrier function, aligns with flow direction and is reconstructed byin situtwo-photon-microscopy. Inflammatory cytokine release (tumor necrosis factorα, stromal-derived factor (1) is demonstrated in both a transwell assay and the 3D model. In proof-of-principle experiments, mesoangioblasts, known as a promising candidate for a stem cell therapy against muscular dystrophies, are injected into the vessel model, showing shear-resistant endothelial adhesion under capillary-like flow conditions. Our 3Din vitromodel offers significant potential to study transendothelial migration mechanisms of stem cells, facilitating the development of improved stem cell therapies.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":9.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141174525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D bioprinting of dense cellular structures within hydrogels with spatially controlled heterogeneity. 在具有空间异质性控制的水凝胶中进行致密细胞结构的三维生物打印。

IF 9 2区医学

Biofabrication Pub Date : 2024-06-11 DOI: 10.1088/1758-5090/ad52f1

Alperen Abaci, Murat Guvendiren

{"title":"3D bioprinting of dense cellular structures within hydrogels with spatially controlled heterogeneity.","authors":"Alperen Abaci, Murat Guvendiren","doi":"10.1088/1758-5090/ad52f1","DOIUrl":"10.1088/1758-5090/ad52f1","url":null,"abstract":"Embedded bioprinting is an emerging technology for precise deposition of cell-laden or cell-only bioinks to construct tissue like structures. Bioink is extruded or transferred into a yield stress hydrogel or a microgel support bath allowing print needle motion during printing and providing temporal support for the printed construct. Although this technology has enabled creation of complex tissue structures, it remains a challenge to develop a support bath with user-defined extracellular mimetic cues and their spatial and temporal control. This is crucial to mimic the dynamic nature of the native tissue to better regenerate tissues and organs. To address this, we present a bioprinting approach involving printing of a photocurable viscous support layer and bioprinting of a cell-only or cell-laden bioink within this viscous layer followed by brief exposure to light to partially crosslink the support layer. This approach does not require shear thinning behavior and is suitable for a wide range of photocurable hydrogels to be used as a support. It enables multi-material printing to spatially control support hydrogel heterogeneity including temporal delivery of bioactive cues (e.g. growth factors), and precise patterning of dense multi-cellular structures within these hydrogel supports. Here, dense stem cell aggregates are printed within methacrylated hyaluronic acid-based hydrogels with patterned heterogeneity to spatially modulate human mesenchymal stem cell osteogenesis. This study has significant impactions on creating tissue interfaces (e.g. osteochondral tissue) in which spatial control of extracellular matrix properties for patterned stem cell differentiation is crucial.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":9.0,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141183945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D bioprinting platform development for high-throughput cancer organoid models construction and drug evaluation. 为高通量癌症类器官模型构建和药物评估开发三维生物打印平台。

IF 9 2区医学

Biofabrication Pub Date : 2024-06-07 DOI: 10.1088/1758-5090/ad51a6

Rui Dai, Wei Chen, You Chen, Jinlong Jin, Shiwei Zhang, Changhua Zhang, Jie Liu

{"title":"3D bioprinting platform development for high-throughput cancer organoid models construction and drug evaluation.","authors":"Rui Dai, Wei Chen, You Chen, Jinlong Jin, Shiwei Zhang, Changhua Zhang, Jie Liu","doi":"10.1088/1758-5090/ad51a6","DOIUrl":"10.1088/1758-5090/ad51a6","url":null,"abstract":"The evaluation of anti-tumor drugs is critical for their development and clinical guidance. Tumor organoid models are gaining increased attention due to their ability to better mimic real tumor tissues, as well as lower time and economic costs, which makes up for the shortcomings of cell lines and xenograft models. However, current tumor organoid cultures based on the Matrigel have limitations in matching with high-throughput engineering methods due to slow gelation and low mechanical strength. Here, we present a novel composite bioink for culturing colorectal cancer organoids that provides an environment close to real tissue growth conditions and exhibits excellent photocrosslinking properties for rapid gel formation. Most importantly, the tumor organoids viability in the composite bioink after printing was as high as 97%, which also kept multicellular polar structures consistent with traditional culture methods in the Matrigel. Using 3D bioprinting with this composite bioink loaded with organoids, we demonstrated the feasibility of this drug evaluation model by validating it with clinically used colorectal cancer treatment drugs. Our results suggested that the composite bioink could effectively cultivate tumor organoids using 3D bioprinting, which had the potential to replace less reliable manual operations in promoting the application of tumor organoids in drug development and clinical guidance.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":9.0,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141174517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0