Biofabrication最新文献

{"title":"A preclinical large-animal evaluation of a 3D bioprinted bionic pancreatic tissue model built on simplified vascular architecture.","authors":"Michał Wszoła, Andrzej Berman, Marta Klak, Tomasz Dobrzanski, Sylwester Domański, Katarzyna Woźniak, Oliwia Janowska, Anna Papierniak-Wyglądała, Agnieszka Górnik, Przemyslaw Wrochna, Dominika Ujzadowska, Dominika Piątek, Jarosław Wejman, Dominika Szkopek, Katarzyna Roszkowicz-Ostrowska, Kacper Nowak, Robert Pasławski, Urszula Pasławska, Lukasz Kownacki, Piotr Cywoniuk, Jarosław Woliński, Agnieszka Dobrzyń, Artur Kamiński, Wojciech Święszkowski","doi":"10.1088/1758-5090/ae7831","DOIUrl":"10.1088/1758-5090/ae7831","url":null,"abstract":"<p><p>Three-dimensional (3D) bioprinting enables the fabrication of complex tissue constructs with integrated vascular architectures, offering promising applications in transplantation. It is commonly assumed that dense, pre-formed microvascular networks are required to ensure survival and function of bioprinted organs immediately after implantation. This<i>in vivo</i>study aimed to evaluate the feasibility of bioprinting and transplanting a vascularized pancreatic tissue construct featuring a single perfusable vessel in a porcine model, with a focus on maintaining stable perfusion and supporting post-transplantation microvascular development. The study included 14 pigs, in which a 3D-bioprinted construct was transplanted and connected to the iliac artery or abdominal aorta. Two vascular connection strategies were evaluated: the Decell group (<i>n</i>= 5), using decellularized and recellularized external vessels for anastomosis, and the Bionic group (<i>n</i>= 9), using a vascular prosthesis. Immediate perfusion was achieved in all transplanted constructs. Three animals from the Bionic group completed a 4 week follow-up period. Computed tomography imaging and histopathological analyses demonstrated sustained perfusion of the constructs and progressive development of microvascular structures within the bioprinted tissue over time. These findings demonstrate the feasibility of fabricating and transplanting a perfusable, mechanically stable, bioprinted pancreatic tissue construct capable of supporting<i>in vivo</i>vascular remodeling without a pre-fabricated dense capillary network. This work provides a preclinical proof-of-feasibility for simplified vascular design strategies in bioprinted tissue constructs and supports their further development toward translational applications.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148161579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generic optimization procedure for high-resolution printing by stereolithography of 3D scaffolds compatible for cell culture applied to a small intestinal architecture.","authors":"Elise Ponthier, Lucien Guth, Sandra Pérez-Domínguez, Julie Foncy, Daniel Ferri-Angulo, Arnaud Besson, Laurent Malaquin","doi":"10.1088/1758-5090/ae7c36","DOIUrl":"10.1088/1758-5090/ae7c36","url":null,"abstract":"<p><p>Stereolithography is now widely used in tissue engineering since it allows fine-tuning of geometry and mechanical properties to mimic the topography of tissues. However, most published studies do not detail the optimization protocols that have been used to obtain these structures. Here, we developed a generic process to print photosensitive biomaterial formulations by stereolithography to obtain a specific topography. Using the small intestine architecture as a model combining filled villi and hollow crypts, we identify key parameters that govern both printing resolution and cellular behavior. Moreover, we highlight the impact of absence of oxygen inhibition and light penetration depth in cavities, causing partially crosslinked material within crypts. This issue was resolved by adding tartrazine, a biocompatible photoabsorber that restricts crosslinking depth. Our stereolithography process allows the fabrication of intestinal models with a high precision level and the reproduction of crypt and villi structures with physiologic dimensions and high structural integrity. We show that Caco-2 cells adhere, colonize, polarize and differentiate on the printed scaffolds over 20 d of culture. The developed models could subsequently be adapted to support intestinal organoid growth and be integrated in microfluidic chips to implement mechanical and biochemical cues, representing a more physiological model with accurate dimensions to study intestinal diseases. Overall, this study provides a transferable framework for optimizing stereolithographic printing of hydrogel-based topographies allowing precise control of substrate properties for tissue engineering.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148223159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune-driven stromal inflammation in pancreatic cancer within a microfluidic platform.","authors":"Mariana Viso, Karthik Rajkumar, Anna Bianchi, Bhumi Suthar, Neil Kumar, David Oliver, Mya Collins, Jashodeep Datta, Ashutosh Agarwal","doi":"10.1088/1758-5090/ae7b09","DOIUrl":"10.1088/1758-5090/ae7b09","url":null,"abstract":"<p><p>Pancreatic ductal adenocarcinoma (PDAC) is defined by a highly fibrotic and immunosuppressive tumor microenvironment (TME). The inflammatory polarization of cancer-associated fibroblasts (CAFs), as well as the dense and early trafficking of myeloid-derived suppressor cells (MDSCs) contribute to tumor progression, immune exclusion, and therapy resistance. Yet, key mechanisms governing their interplay remain undefined. We developed a novel microfluidic platform that recapitulates the immuno-stromal PDAC microenvironment and enables controlled co-culture, high-resolution live imaging, and downstream molecular analyses. Spheroids composed of tumor cells, and dual reporter CAFs, were embedded in our engineered system and allowed to dynamically interact with MDSCs for 48 h. Dynamic interactions with MDSCs induce a significant increase in inflammatory CAF-specific reporter fluorescence compared to media controls. Gene set enrichment analysis demonstrated enrichment of inflammatory response pathways, and increased expression of iCAF related genes. RNA velocity, CellRank, and PAGA trajectory analyses collectively identify a constitutively fate-committed CAF progenitor population that is selectively expanded by MDSC exposure and preferentially transitions into an inflammatory iCAF state. Overall, our novel microfluidic platform recapitulated targeted MDSC-driven inflammatory CAF polarization and revealed a progenitor CAFs population as a potential precursor for iCAF differentiation, establishing its utility in mechanistic studies of stromal-immune interactions of the TME.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13308500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148210034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topology optimization of 3D-printed mycelium hydrogels.","authors":"Winston Lindqwister, Mrinal Chaudhury, Sarah Schyck, Iuri Rocha, Kunal Masania, Martin Lesueur","doi":"10.1088/1758-5090/ae7835","DOIUrl":"10.1088/1758-5090/ae7835","url":null,"abstract":"<p><p>As we move towards more sustainable and resilient materials, new opportunities for harnessing the next generation of biological materials will arise. Materials composed of living organisms have great potential in fulfilling this role as a self-healing, lightweight and sustainable structural material. Recent advances in 3D-printing using fungi-inoculated hydrogels opens the potential of additive manufacturing with fungi into optimized shapes. However, while this technique of 3D-printing fungi has great potential in a wide range of engineering applications, computational models do not yet exist to precisely engineer the strength of structures made from this material. Here we create a computational modeling scheme for 3D-printed mycelium structures, linking the growth of fungi to stiffness. We first model the growth of fungi through a diffusion model. We then convert the resultant density values into local stiffness, creating a computational representation of the varying elemental stiffness as a function of local mycelial density. We implement two Bayesian optimization-based topology optimization schemes to maximize the strength of cuboid 3D-printed structures while minimizing the input material cost. One maximizes the material specific stiffness while the other applies a constrained scheme for identifying a minimized mass for a target design stiffness. Both show a distinct tradeoff in print mass to stiffness, with results validated experimentally. These new insights provide important next steps in the effective harnessing of this class of emergent material, as well as its larger adoption for engineering applications.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148161542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D printed trichome-inspired permeable bioadhesive for wearable bioelectronics.","authors":"Zhen Gu, Jingwen Xu, Heng An, Zhichao Dong, Xinhuan Wang, Yongqiang Wen, Qi Gu","doi":"10.1088/1758-5090/ae7b08","DOIUrl":"10.1088/1758-5090/ae7b08","url":null,"abstract":"<p><p>Wearable bioelectronics that adhere directly to the skin have broad applications. However, achieving optimal breathability remains a significant challenge because of sweat accumulation at the device-skin interface. Conventional approaches, such as porous structures, often limit the functional versatility of wearable bioelectronics. To address this gap, we propose a sweat-removable skin sticker (SRSS) with a hierarchical trichome-inspired channel architecture that rapidly removes sweat from the interface while maintaining robust skin adhesion. The SRSS was fabricated through a hybrid process, in which the trichome-inspired hierarchical microchannel architecture was created by direct ink writing, enabling the controlled deposition of viscous ink with high structural fidelity. Through the multi-level ribs design, the SRSS demonstrated an effective area-normalized horizontal water removal rate (25.6 ml cm^-2min^-1) significantly higher than the human sweat secretion rate (0.38-2.85 × 10^-3ml cm^-2min^-1)-approximately three orders of magnitude. This feature reduces sweat accumulation in wearable bioelectronics, thereby enhancing user comfort. Unlike conventional porous materials, the SRSS relies on channel based adhesive interface design that remains compatible with attached wearable bioelectronics, such as temperature sensors in real time. This work therefore presents a structurally engineered permeable bioadhesive interface for wearable bioelectronics.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148209911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel drug screening strategy based on non-invasive electrochemical detection of multiple tumor markers secreted by patient-derived microgel organoids.","authors":"Chuhan Lv, Hongbo Yu, Yingshi Su, Xiaoyong Dai, Jianwei Chen, Tao Xu, Hua Dong","doi":"10.1088/1758-5090/ae7b0a","DOIUrl":"10.1088/1758-5090/ae7b0a","url":null,"abstract":"<p><p>Rapid and effective drug screening is a key step for personalized cancer therapy. Recently, microgel organoids have emerged as a promising<i>in vitro</i>drug screening model that ensures not only the uniform manufacture, but also the integration of various cells, active factors and matrix materials to mimic the real 3D tumor tissue. However, their corresponding drug sensitivity and efficacy analysis remains challenging, since traditional optical methods are invasive/destructive to tumor organoids and thus require many clinical samples for drug screening. Herein we propose a novel strategy based on non-invasive electrochemical detection of multiple tumor markers secreted by patient-derived microgel organoids. Specifically, a detection electrode is constructed using Ti₃C₂MXene, Au nanoparticles (AuNPs) and aptamers (conjugated with redox probes) to detect breast cancer biomarkers through the specific recognition between aptamer and biomarkers. Our data reveal that high sensitivity and specificity of biomarker sensing can be both achieved for drug screening models prepared using breast cancer cell line, clinical breast cancer and colorectal cancer samples, which can be converted into relative cell viability and half-maximal inhibition concentration (IC₅₀). Similar results between optical and electrochemical methods prove the feasibility and potential application of our strategy in high-throughput drug screening for diverse cancers.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148209883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial capillaries-on-a-chip with modular control over lumen size, architecture, and co-culture conditions.","authors":"Arun Poudel, Joshua Edwin Nana Limjuico, Ujjwal Aryal, Md Tariqul Hossain, Saikat Basu, Pranav Soman","doi":"10.1088/1758-5090/ae7834","DOIUrl":"10.1088/1758-5090/ae7834","url":null,"abstract":"<p><p>Currently,<i>in vitro</i>models of microvascular biology rely on self-assembly of vascular cells in compatible gels. However, the stochastic nature of this process results in large variations in lumen sizes, perfusion continuity, and shear stresses, making systematic and reproducible analysis challenging. Here, we report a new technology to generate artificial capillaries on a chip with custom control over lumen sizes and architectures using a combination of femtosecond laser cavitation and collagen casting within multi-chambered microfluidic chips. The design allows seeding of endothelial cells within capillary-sized microchannels and seeding of stromal cells within top-open silos, with independent control over seeding sequence and media compositions. Results show that endothelialized microchannels, coined as artificial capillaries, exhibit excellent barrier function with reproducible control over lumen sizes (<i>φ</i>= 8-40<i>µ</i>m) and their architectures (straight, curvatures, tapered, branched). The physical flow parameters measured across the lumen (namely, flow shear) and at the channel outlets (flow velocities) have been validated against high-fidelity numerical assessments from the Large Eddy Simulation scheme within the digitized versions of microchannels. The experiment-computation compatibility enabled us to predict changes in regional velocity and wall shear stresses within artificial capillaries for various capillary architectures. We also show that<i>in situ</i>editing of artificial capillaries in the form of adding new branches or adding occlusions is possible. Lastly, we developed a co-culture model that enables the study of stromal cells with artificial capillaries using conventional imaging methods. We envision that acellular chips with two seeding ports can be readily shipped worldwide and could potentially be adopted as a new technology to study microvascular biology in a reproducible manner.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13276706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148161571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Large-scale manufacturing of precisely patterned flexible soft tissue implants with high porosity.","authors":"Amal Shabazz, Alexandra P Christensen, David Garvey, Mike Pallotta, Sahar Vakili, Stephen Farias, John P Fisher","doi":"10.1088/1758-5090/ae7697","DOIUrl":"10.1088/1758-5090/ae7697","url":null,"abstract":"<p><p>As improvements in material compatibility are achieved, stereolithography (SLA) 3D printing allows for the prototyping of soft tissue constructs with high reproducibility and precision. To streamline the Food and Drug Administration approval process, clinical-grade manufacturing must be validated. Here, we present a manufacturing process and post-manufacturing analysis for soft-tissue implants used to reconstruct the nipple-areolar complex (NAC). The NAC is a specialized soft tissue structure that influences aesthetic outcomes in breast reconstruction. Physiologically-sized NAC implants were designed and printed in a good manufacturing practice facility using Formlabs Biomed Elastic 50 A (E 50 A) and Biomed Flexible 80 A (F 80 A) resins. Documentation and procedures were established to standardize and control macroporous soft-tissue implant manufacturing. Post-manufacturing classification based on architectural and mechanical features defined the minimum acceptable criteria for future production. E 50 A macroporous implants resembled the modulus of soft tissue, while F 80 A geometries showed higher reproducibility and preservation of fine architectural features. SLA printing enabled the patterning of implants with porosities of 74.65% and 72.74% for 0.4- and 0.5- mm geometries, respectively. 3D printed implants supported the infusion of gelatin methacryloyl for the delivery of biological cues, with a filling efficiency greater than 97%. This manufacturing platform achieves reproducible fabrication of soft-tissue implants that meet both structural and mechanical requirements. Further, the presented methodology can be adapted for a wide range of complex soft tissues to advance regenerative medicine solutions toward regulatory approval and eventual clinical integration.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13266584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148148310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phototunable hydrogel mechanics for spatial guidance of blood capillary morphogenesis.","authors":"He Li, Hao Liu, Fiona Louis, Oju Jeon, Eben Alsberg, Marcy Zenobi-Wong, Michiya Matsusaki","doi":"10.1088/1758-5090/ae7509","DOIUrl":"10.1088/1758-5090/ae7509","url":null,"abstract":"<p><p>Creating large, functional tissue requires the engineering of a blood capillary network, which is challenging as it necessitates the recapitulation of capillary morphology and spatial distribution. Current approaches employ local mechanical and chemical gradients to modulate capillary morphology or the network's density but they often fail to regulate both parameters to fulfill the needs of tissue engineering. Here, we present a light-mediated biomaterial platform based on the FLight biofabrication technique to regulate blood capillary alignment and density. By modulating local light doses, a highly aligned hydrogel microstructure with spatially tunable matrix stiffness was achieved within a single 3D hydrogel construct. The anisotropic microstructures guided blood capillary elongation and alignment, achieving over 90% alignment in matrices (3.4-5.4 kPa), while spatial variations in stiffness regulated vascularization. Blood capillary density was maximized in regions with intermediate stiffness (4.6-5.0 kPa) and markedly lower in softer (∼2.0 kPa) and stiffer (∼5.4 kPa) matrices. This study establishes spatial light dosage as an effective engineering parameter for programming matrix mechanics and microvascular organization within a single hydrogel construct, providing a scalable strategy for fabricating anisotropic and spatially heterogeneous capillary networks for vascularized tissue engineering.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.0,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148052307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Principle-based multiphysics simulation for 3D bioprinting systems: modelling inkjet, extrusion, and DLP processes.","authors":"Yunong Yuan, Ahmad Fahmi Anwar Fadzil, Chloe Choi, Tae-Joon Jeon, Yiqiao Hu, Jinhui Wu, Nezamoddin N Kachouie, Lifeng Kang","doi":"10.1088/1758-5090/ae6ad0","DOIUrl":"10.1088/1758-5090/ae6ad0","url":null,"abstract":"<p><p>Among additive manufacturing (AM), 3D inkjet technology, materials extrusion (ME), and digital light processing (DLP), which are from dot and line to face printing, have been extensively investigated for biological and pharmaceutical applications. These techniques are valued for their ability to create customised, complex, drug-laden devices and tissue engineering scaffolds. However, testing new bioinks or filament designs can be both expensive and time-consuming. To this end, numerical simulation offers a useful solution by reducing costs and saving time. Both machine learning (ML) and theory-based models can be used for simulation. ML excels in handling complex data but faces challenges with data availability and overfitting, while theory-based models provide a more interpretable and data-efficient framework. This review explores how theory-based numerical simulation can be used to assess and optimise factors such as bioink printability, technique mechanism, printing parameters, and post-printing outcomes. By using simulation, key parameters can be understood and optimised without performing extensive physical experiments. The review highlights current models and discusses opportunities and challenges in using simulations to enhance the AM process, potentially advancing regenerative medicine and personalised treatments.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.0,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147855776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}