Biofabrication最新文献

Tailoring agarose fluid gels for use in suspension bath bioprinting and culture of spheroid-based bioinks. 用于悬浮浴生物打印和球形生物墨水培养的定制琼脂糖流体凝胶。

IF 8 2区医学

Biofabrication Pub Date : 2025-10-10 DOI: 10.1088/1758-5090/ae0aff

Megan E Cooke, Nikolas Di Caprio, Jason Killgore, Jason A Burdick

{"title":"Tailoring agarose fluid gels for use in suspension bath bioprinting and culture of spheroid-based bioinks.","authors":"Megan E Cooke, Nikolas Di Caprio, Jason Killgore, Jason A Burdick","doi":"10.1088/1758-5090/ae0aff","DOIUrl":"10.1088/1758-5090/ae0aff","url":null,"abstract":"Suspension bath bioprinting, whereby bioinks are extruded into a yield stress bath with rapid recovery from shearing, has enabled the printing of low viscosity bioinks into constructs with high geometric complexity. Previous studies have often relied upon external stabilisation of the suspension bath (e.g. collagen) in order to culture soft materials without loss of printed structure. Here, we report a systematic investigation of suspension bath properties that support the printing, fusion, and culture of spheroid-based bioinks without added stabilisation. Specifically, agarose fluid gels of varied polymer concentrations and dilutions were produced and characterised morphologically and rheologically. Juvenile bovine chondrocytes or mesenchymal stromal cells (MSCs) were formed into spheroids of ∼150µm in diameter and investigated within agarose suspension baths either for their fusion in hanging drop cultures or as jammed bioinks. MSC spheroids were also printed when mixed with hydrogel microparticles to demonstrate additional versatility to the approach. Suspension baths of lower polymer concentrations and increased dilution enabled faster spheroid fusion; however, the most heavily diluted suspension bath was unable to maintain print fidelity. Other formulations supported the printing, fusion, and culture of spheroid-based inks, either as simple lines or more complex patterns. These findings help to inform the design of suspension baths for bioprinting and culture.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.0,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145135937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofabrication of an ovine intervertebral disc model by combining a polycaprolactone frame with a bioprinted alginate hydrogel. 结合聚己内酯框架和生物打印海藻酸盐水凝胶的羊椎间盘模型的生物构建。

IF 8 2区医学

Biofabrication Pub Date : 2025-10-10 DOI: 10.1088/1758-5090/ae0c3e

Emmaëlle Carrot, Mansoor Chaaban, Daronne Cano Contreras, Clara Schiex, Joëlle Véziers, Boris Halgand, François Loll, Johann Clouet, Michael G Monaghan, Marion Fusellier, Jérôme Guicheux, Vianney Delplace, Catherine Le Visage

{"title":"Biofabrication of an ovine intervertebral disc model by combining a polycaprolactone frame with a bioprinted alginate hydrogel.","authors":"Emmaëlle Carrot, Mansoor Chaaban, Daronne Cano Contreras, Clara Schiex, Joëlle Véziers, Boris Halgand, François Loll, Johann Clouet, Michael G Monaghan, Marion Fusellier, Jérôme Guicheux, Vianney Delplace, Catherine Le Visage","doi":"10.1088/1758-5090/ae0c3e","DOIUrl":"10.1088/1758-5090/ae0c3e","url":null,"abstract":"The intervertebral disc (IVD) primarily comprises an outer ring of collagen fibers (annulus fibrosus, AF), which encases a soft, gelatinous core (nucleus pulposus, NP). Existingin vitromodels have failed to integrate these two tissues effectively or accurately replicate their intricate organization. By combining two biofabrication techniques, we developed a novel 3Din vitromodel that closely mimics the organization of an ovine IVD. Our approach employs a polycaprolactone (PCL) frame produced via melt electrowriting to recreate the multilamellar architecture of the AF. Ovine primary cells, encapsulated in a photocrosslinkable alginate hydrogel, were precisely extruded within the multilamellar structure, thereby mimicking the native shape and size of an ovine disc. The bioink containing the NP cells was deposited at the center of the construct, while the bioink with the AF cells was strategically layered in between the lamellae of the PCL frame. Photocrosslinking was optimized to match the native stiffness of the disc. The constructs were maintained in culture for 28 d, during which we thoroughly assessed reproducibility, stability, and cell viability and phenotype. The results unequivocally demonstrated that the PCL frame effectively guided the alignment and proliferation of AF cells, while the alginate hydrogel preserved NP cell phenotype. This model successfully replicates the organization of the IVD, providing a promising platform for advancing our understanding of disc biology and driving the development of novel therapeutic strategies.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.0,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reimagining bioprinters: real-time monitoring for quality control of bioprinted constructs and future vision. 重塑生物打印机：实时监测生物打印结构的质量控制和未来愿景。

IF 8 2区医学

Biofabrication Pub Date : 2025-10-10 DOI: 10.1088/1758-5090/ae11d4

Alicia Adina Matavosian, Lawrence Bonassar

{"title":"Reimagining bioprinters: real-time monitoring for quality control of bioprinted constructs and future vision.","authors":"Alicia Adina Matavosian, Lawrence Bonassar","doi":"10.1088/1758-5090/ae11d4","DOIUrl":"https://doi.org/10.1088/1758-5090/ae11d4","url":null,"abstract":"The use of bioprinters as depositional tools for bioinks and cells has expanded greatly over the past two decades. Bioprinting combines hydrogels with cells to produce customized constructs for personalized medicine. However, several challenges hinder the clinical use of these constructs. Quality control metrics for bioprinting involve the assessment of critical quality attributes (CQAs) at every stage of production. Currently, bioprinted constructs are manually assessed using destructive methods that occur post-production, requiring the creation of multiple products per patient. Reproducing printed constructs is difficult due to time-sensitive bioink properties that require lengthy optimization processes to print with accuracy. In addition, the collection, processing, and testing of cell bioactivity for each printed construct greatly increases production costs. To address these challenges, non-destructive, real-time monitoring can be integrated into the bioprinting process.The goal of this review paper is to reimagine the function of a bioprinter from a simple tool of production to one capable of evaluating constructs in real-time. This review features recent advances in the field for real-time monitoring with a focus on time-sensitive bioink properties, print accuracy, and cell health. Automated assessment and quantification of time-sensitive bioink qualities such as mixing, pH, temperature, and viscosity will enhance construct quality by enabling the rapid optimization of printing parameters. Meanwhile, real-time monitoring of cell health through concentration, viability, and type serves as an indicator for bioactivity. Construct accuracy and reproducibility are also improved through the identification, prediction, and correction of defects during printing. Incorporating real-time monitoring into the bioprinting process using closed-loop feedback would improve the reproducibility, quality, and translation of constructs into the clinic.&#xD.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.0,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D TPMS curvature accelerated osteogenesis by enhancing permeability and directing cell orientation. 三维TPMS曲率通过增强渗透性和引导细胞定向来加速成骨。

IF 8 2区医学

Biofabrication Pub Date : 2025-10-09 DOI: 10.1088/1758-5090/ae1166

Jiamian Han, Heming Chen, Jiayi Li, Qiang Chen, Hongcheng Gu, Zhongze Gu

{"title":"3D TPMS curvature accelerated osteogenesis by enhancing permeability and directing cell orientation.","authors":"Jiamian Han, Heming Chen, Jiayi Li, Qiang Chen, Hongcheng Gu, Zhongze Gu","doi":"10.1088/1758-5090/ae1166","DOIUrl":"https://doi.org/10.1088/1758-5090/ae1166","url":null,"abstract":"The curvature of cell adhesion substrates has emerged as a critical geometric parameter influencing cellular fate determination. While its regulatory role is increasingly recognized, the osteogenic effects of complex three-dimensional curved surfaces remain insufficiently explored. In this study, high-precision two-photonic polymerization 3D printing was utilized to fabricate scaffolds with controlled curvature distributions, achieving unprecedented fidelity between manufactured surfaces and their digital models. Comparative analysis of triply periodic minimal surface (TPMS) scaffolds and conventional truss scaffolds revealed distinct osteogenic mechanisms: zero mean curvature enhanced osteogenic differentiation through improved scaffold permeability, while negative Gaussian curvature promoted bone formation through combined effects of permeability controlling and guided cellular organization. Notably, scaffolds exhibiting broader ranges of negative Gaussian curvature demonstrated superior osteogenesis inductive capacity, as evidenced by enhanced new bone formation in both in vitro and in vivo models. These findings provide mechanistic insights into curvature-dependent osteogenesis, quantitative design principles for TPMS-based bone scaffolds, and experimental validation of curvature optimization strategies. The study establishes a geometric framework for rational scaffold design, advancing the development of high-performance regenerative implants.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.0,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A biofabricated 3D cancer-stroma tumor microenvironment model. 生物构建的三维癌间质肿瘤微环境模型。

IF 8 2区医学

Biofabrication Pub Date : 2025-10-07 DOI: 10.1088/1758-5090/ae0a82

Sara Romanazzo, Peilin Tian, Gagan K Jalandhra, Riddhesh B Doshi, J Justin Gooding, Kristopher A Kilian

{"title":"A biofabricated 3D cancer-stroma tumor microenvironment model.","authors":"Sara Romanazzo, Peilin Tian, Gagan K Jalandhra, Riddhesh B Doshi, J Justin Gooding, Kristopher A Kilian","doi":"10.1088/1758-5090/ae0a82","DOIUrl":"10.1088/1758-5090/ae0a82","url":null,"abstract":"Breast cancer progression is a consequence of intricate dynamics between cells and their matrix in the tumor microenvironment. However, mostin vitromodels are not amenable to studying the behavior of multiple cell types within a defined matrix architecture. In this study, we demonstrate a microporous matrix where breast cancer cells and adipose derived stromal cells are integrated to evaluate crosstalk between matrix parameters and heterotypic cell populations. To do this, we leveraged two biofabrication techniques, granular suspension matrices and drop-on-demand bioprinting, to deposit multiple cell types in a reproducible format amenable to high-throughput screening. 3D gelatin-methacryloyl microgels were used to create a yield stress granular suspension bath with tunable interstitial volume to mimic the porosity and densities of healthy and fibrotic microenvironments. Invasive and non-invasive breast cancer cells (MCF-7 and MDA-MB-231) were bioprinted at the interface of the ADSC-laden granular matrix to probe invasive processes and heterotypic crosstalk as a tumor-stroma model. We focused on cancer cell migration through model fibrotic tissue and ADSC transformations into cancer associated fibroblasts.α-smooth muscle actin expression indicated that the high density microgel matrices are more conducive to ADSC-CAF transformations, which in turn influenced the expression of molecular markers associated with tumorigenicity and chemoresistance in the resident cancer cells. Treatment with doxorubicin supported increased tumorigenicity in the co-cultures. Together, this work demonstrates how defined microengineered matrices can serve as platforms to evaluate cell behavior, with scope for translation toin vitroassays for biological discovery and drug development.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12501863/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D bioprinted cell-laden GrooveNeuroTube: a multifunctional platform forex vivoneural cell migration and growth studies. 3D生物打印细胞负载的GrooveNeuroTube：一种用于静脉神经细胞迁移和生长研究的多功能平台。

IF 8 2区医学

Biofabrication Pub Date : 2025-10-07 DOI: 10.1088/1758-5090/ae0550

Jagoda Litowczenko, Yannick Richter, Hawrez Ismael, Łukasz Popenda, Adam Ostrowski, Katarzyna Fiedorowicz, Jose Carlos Rodrigez Cabello, Jacek K Wychowaniec, Krzysztof Tadyszak

{"title":"3D bioprinted cell-laden GrooveNeuroTube: a multifunctional platform forex vivoneural cell migration and growth studies.","authors":"Jagoda Litowczenko, Yannick Richter, Hawrez Ismael, Łukasz Popenda, Adam Ostrowski, Katarzyna Fiedorowicz, Jose Carlos Rodrigez Cabello, Jacek K Wychowaniec, Krzysztof Tadyszak","doi":"10.1088/1758-5090/ae0550","DOIUrl":"10.1088/1758-5090/ae0550","url":null,"abstract":"Extensive peripheral nerve injuries often lead to the loss of neurological function due to slow regeneration and limited recovery over large gaps. Current clinical interventions, such as nerve guidance conduits (NGCs), face challenges in creating biomimetic microenvironments that effectively support nerve repair. The developedGrooveNeuroTubeis composed of hyaluronic acid methacrylate and gelatin methacrylate hydrogel, incorporating active agents (growth factors and antibacterial agents) encapsulated within an NGC conduit made of 3D-printed PCL grid fibers.In vitrostudies showed thatGrooveNeuroTubesignificantly promoted migration of dorsal root ganglion (DRG) neuronal cells, 3D bioprinted at the far ends of the conduit to imitate a proximal nerve injury as a novelex vivomodel. A long-term culture of up to 60 d was employed to better mimicin vivoconditions. This model tested the effects of pulsed electromagnetic field stimulation on neural tissue development. After 60 d,GrooveNeuroTubeshowed a 32% cell migration increase compared to the growth-factor-group and 105% compared to the no-growth-factor condition. These results confirm that theGrooveNeuroTubesystem can effectively support sustained neural cell migration and maturation over extended periods, proving a new technology for testing peripheral nerve injuryex vivo.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liver-on-Micropillar: A Humanized, Animal-Free Platform for High-Throughput Assessment of Drug-Induced Liver Injury. 肝微柱：一个高通量评估药物性肝损伤的人性化、无动物平台。

IF 8 2区医学

Biofabrication Pub Date : 2025-10-07 DOI: 10.1088/1758-5090/ae1063

Ahmed S M Ali, Dongwei Wu, Viola Röhrs, Duy Bao Tran Nguyen, Mathias Ziersch, Albert Braeuning, Jens Kurreck

{"title":"Liver-on-Micropillar: A Humanized, Animal-Free Platform for High-Throughput Assessment of Drug-Induced Liver Injury.","authors":"Ahmed S M Ali, Dongwei Wu, Viola Röhrs, Duy Bao Tran Nguyen, Mathias Ziersch, Albert Braeuning, Jens Kurreck","doi":"10.1088/1758-5090/ae1063","DOIUrl":"10.1088/1758-5090/ae1063","url":null,"abstract":"Drug-induced liver injury (DILI) remains a major cause of acute liver failure, clinical trial attrition, and post-marketing drug withdrawal, yet predictive in vitro models are limited in accuracy, scalability, and human relevance. Here, we present a Liver-on-Micropillar (LoM) platform a fully animal-free, high-throughput, miniaturized human liver model designed for early-stage hepatotoxicity screening. The system combines a xeno-free medium (XFM) with a xeno-free bioink to support co-culture of four human liver-relevant cell types: differentiated HepaRG, LX-2, HMEC-1, and differentiated THP-1 cells. Microlivers are bioprinted onto micropillar arrays compatible with standard 96-well plate formats. Functional characterization confirmed stable cell viability, albumin and urea production, as well as inducible CYP expression. To evaluate DILI predictivity, ten reference drugs were tested using assays to measure ATP content, XTT metabolic activity, and albumin secretion. Half-maximal inhibitory concentrations (IC50) were experimentally determined, and margins of safety (MOS) were calculated by dividing IC50 by clinical maximum plasma concentration (Cmax). The LoM platform correctly classified 90% of the tested compounds using a MOS threshold of 100. This scalable and reproducible model provides a human-relevant, regulatory-aligned alternative to animal testing and supports broader efforts to implement non-animal methodologies in drug safety evaluation.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145243418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Organ to System: Multiorgan-on-a-Chip Platforms as Next-Generation Biomedical Simulators. 从器官到系统：作为下一代生物医学模拟器的多器官芯片平台。

IF 8 2区医学

Biofabrication Pub Date : 2025-09-30 DOI: 10.1088/1758-5090/ae0dba

Chen Chen, Lin Zhou, Ni Kou, Xue Li, Hongju Mao, Huiying Liu

{"title":"From Organ to System: Multiorgan-on-a-Chip Platforms as Next-Generation Biomedical Simulators.","authors":"Chen Chen, Lin Zhou, Ni Kou, Xue Li, Hongju Mao, Huiying Liu","doi":"10.1088/1758-5090/ae0dba","DOIUrl":"https://doi.org/10.1088/1758-5090/ae0dba","url":null,"abstract":"Organs in the human body exist within a highly integrated and dynamically interacting environment, and their interactions are critical for maintaining normal physiological processes. Traditional cell culture models and animal models fail to meet the needs of preclinical research, as they struggle to fully recapitulate in vivo physiology and pathology. Thus, innovative in vivo platforms are urgently needed to bridge the gaps between preclinical research and clinical translation. Multiorgan-on-a-chip (multi-OoC), an emerging field in bioengineering, offers precise control over cellular microenvironments and recapitulates organ-level functions and interorgan crosstalk. By mimicking complex human physiology and pathophysiology, multi-OoC systems provide novel opportunities for disease modeling, drug discovery, and personalized medicine. This paper will systematically elaborate on the necessity of developing multi-OoC systems, delve into their structural design and biomanufacturing strategies, and highlight their recent applications in biomedical research. Additionally, it will analyze key challenges such as the establishment of standardized operating procedures and the validation of model outputs, and envision their application prospects in the field of personalized medicine. The aim is to provide a reference for promoting the standardization and clinical translation of this technology.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Embolization-on-a-chip: novel vascularized liver tumor model for evaluation of cellular and cytokine response to embolic agents. 芯片栓塞：用于评估细胞和细胞因子对栓塞剂反应的新型血管化肝肿瘤模型。

IF 8 2区医学

Biofabrication Pub Date : 2025-09-29 DOI: 10.1088/1758-5090/adfbc3

Huu Tuan Nguyen, Zuzana Tirpakova, Arne Peirsman, Surjendu Maity, Natashya Falcone, Satoru Kawakita, Keuna Jeon, Danial Khorsandi, Ahmad Rashad, Neda Farhadi, Kalpana Mandal, Menekse Ermis, Rondinelli Donizetti Herculano, Alireza Hassani Najafabadi, Mehmet Remzi Dokmeci, Natan Roberto De Barros, Ali Khademhosseini, Vadim Jucaud

{"title":"Embolization-on-a-chip: novel vascularized liver tumor model for evaluation of cellular and cytokine response to embolic agents.","authors":"Huu Tuan Nguyen, Zuzana Tirpakova, Arne Peirsman, Surjendu Maity, Natashya Falcone, Satoru Kawakita, Keuna Jeon, Danial Khorsandi, Ahmad Rashad, Neda Farhadi, Kalpana Mandal, Menekse Ermis, Rondinelli Donizetti Herculano, Alireza Hassani Najafabadi, Mehmet Remzi Dokmeci, Natan Roberto De Barros, Ali Khademhosseini, Vadim Jucaud","doi":"10.1088/1758-5090/adfbc3","DOIUrl":"10.1088/1758-5090/adfbc3","url":null,"abstract":"Blood vessel embolization is a well-established treatment modality for liver cancer. Novel shear-thinning hydrogels (STH) have been developed to address the need for safer and more effective local delivery of embolic agents and therapeutics. However, embolization therapies are currently optimized in animal models, which often differ from humans at the cellular, tissue, and organ levels. We aim to evaluate the efficacy of novel embolic agents such as STH using a human-relevantin vitromodel that recapitulates human hepatocellular carcinoma capillary networks. A vascularized human liver-tumor-on-a-chip model was developed to assess embolic agent performance. The effects of drug-eluting STH (DESTH) on tumor cell viability, surface marker expression, vasculature morphology, and cytokine responses were evaluated. To study the effects of embolization on microvasculature morphology independent of the chemotherapy compound, we evaluated the effect of different drug-free embolic agents on the vascular tumor microenvironment under flow conditions. DESTH treatment induced tumor cell death, downregulated the expression of epithelial cell adhesion molecules in HepG2, increased levels of cytokines such as interleukin-4 (IL-4), granulocyte-macrophage colony-stimulating factor, and vascular endothelial growth factor, and decreased albumin secretion. Furthermore, different embolic agents exert distinct effects on microvascular morphology, with STH causing complete regression of the microvascular networks. This vascularized liver tumor-on-a-chip model enables human-relevant, real-time assessment of embolic agent efficacy and vascular response and can be applied for the development of innovative and effective embolization therapies for liver cancer.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tumor organoid and tumor-on-a-chip equipped next generation precision medicine. 肿瘤类器官和肿瘤芯片装备的下一代精准医学。

IF 8 2区医学

Biofabrication Pub Date : 2025-09-25 DOI: 10.1088/1758-5090/ae0bb3

Wentao Zhao, Zilin Zhang, Shihui Xu, Xuan Sun, Yan Wang, Jiexian Ye, Xinling Wang, Zhongze Gu, Zaozao Chen

{"title":"Tumor organoid and tumor-on-a-chip equipped next generation precision medicine.","authors":"Wentao Zhao, Zilin Zhang, Shihui Xu, Xuan Sun, Yan Wang, Jiexian Ye, Xinling Wang, Zhongze Gu, Zaozao Chen","doi":"10.1088/1758-5090/ae0bb3","DOIUrl":"https://doi.org/10.1088/1758-5090/ae0bb3","url":null,"abstract":"Recently, significant advancements have been witnessed in various in vitro treatment evaluation models, especially organoids and organs-on-chips. In vitro culture of cancer cells and drug screening are key technical components in functional oncology precision medicine. However, most studies primarily focus on constructing models using established cell lines, with limited integration with clinical diagnosis or patient treatment. This review provides a brief overview of precision medicine models, followed by discussions on the broad spectrum of applications involving two-dimensional (2D) tumor cell culture, patient-derived tumor xenograft (PDTX) models, tumor organoids, and tumors-on-chips. It highlights the success rate of patient-derived tumor organoids (PDTOs) construction and their application in clinical trials. Recent advancements in tumors-on-chips and organoids-on-chips are elaborated on, alongside with integration of other new generation technologies. Additionally, this review summarizes the advantages and constraints associated with tumor organoids and tumors-on-chips, underscoring their crucial role in the advancement of personalized medicine.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145147573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0