Biofabrication最新文献

{"title":"Engineered tumor microspheres via microfluidics and decellularized extracellular matrix for high-throughput organoid-based drug screening.","authors":"Jinlong Jin, Wei Chen, Jing Li, Jiahuan Yang, Rui Dai, Junjie Tang, Meiqi Li, You Chen, Changhua Zhang, Jie Liu","doi":"10.1088/1758-5090/adf099","DOIUrl":"10.1088/1758-5090/adf099","url":null,"abstract":"<p><p>Colorectal cancer is a prominent global malignancy that highlights the pressing need for reliable preclinical models to expedite therapeutic efficacy and drug discovery. Traditional models, such as cell lines and patient-derived xenografts, are constrained by their inability to fully replicate tumor heterogeneity and support scalable drug screening. While patient-derived organoids more accurately preserve tumor pathophysiology, their clinical translation is impeded by technical challenges related to standardization, reproducibility, and high-throughput compatibility. In this study, we developed a microfluidic-engineered platform that employed a laminin-enhanced decellularized small intestinal submucosa extracellular matrix (dSISML) to produce uniform organoid-laden microspheres (MP). This biohybrid system eliminated the need for tumor-derived matrices (e.g. Matrigel) and provided a physiologically relevant microenvironment. When integrated with microfluidics, the platform facilitated rapid and scalable production of size-tunable MP, thereby effectively addressing critical bottlenecks in organoid handling and drug testing workflows. Our study demonstrated that dSISML could sustain organoid growth and drug responsiveness comparable to Matrigel, while offering improved operational simplicity and reduced batch variability. Moreover, dSISML enabled simpler and controllable high-throughput microsphere preparation. This advanced methodology not only delivers precision equivalent to conventional cell culture techniques but also empowers large-scale pharmacological evaluation through its automated media processing system. By integrating biomimetic design with scalable fabrication, this strategy advances personalized oncology through robust<i>in vitro</i>models for high-throughput therapeutic screening and mechanistic studies.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D bioprinted GelMA/collagen hydrogel for corneal stroma regeneration.","authors":"Yingni Xu, Wenfang Liu, Qi Zhao, Xiaoyan Feng, Zhibiao Li, Yongrui Huang, Jia Liu, Yuehai Peng, Wenjing Song, Li Ren","doi":"10.1088/1758-5090/ade7b2","DOIUrl":"10.1088/1758-5090/ade7b2","url":null,"abstract":"<p><p>Blindness caused by corneal stroma disease affects millions worldwide, the regeneration of corneal stroma has always been a challenge due to its sophisticated curvature structure and keratocyte-fibroblast transformation. In this study, we developed and optimized a series of gelatin methacrylate/collagen-based bioinks to fabricate convex corneal implants via 3D printing techniques. A novel method was proposed to enhance collagen solubility in neutral solutions by combining 2,3-epoxypropyltrimethylammonium chloride with high-molecular-weight type I collagen, with simulations suggesting that the mechanism primarily involved electrostatic interactions. To evaluate whether keratocytes respond to a convex microenvironment and to verify the effectiveness of the proposed printing strategy for corneal stromal regeneration, particularly in mitigating corneal fibrosis, we fabricated topological structures of both flat and convex corneas. These structures were systematically analyzed for their influence on keratocyte-to-fibroblast transformation and keratocyte phenotype maintenance. Morphological observations, along with gene and protein expression analyses, demonstrated that the convex architecture provided an optimal microenvironment for preserving the keratocyte phenotype. Moreover,<i>in vivo</i>transplantation revealed the convex cornea effectively suppressed corneal fibrosis compared to the flat cornea. These findings suggest that convex cornea holds promise as a potential translational approach for treating corneal stroma regeneration.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144483100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Review on engineered polymer microneedles for drug delivery and disease diagnosis.","authors":"Yan Li, Zhenyu Wu, Yan Zhou, Sedrati Manar, Rui Wang, Guohua Jiang","doi":"10.1088/1758-5090/adeecc","DOIUrl":"10.1088/1758-5090/adeecc","url":null,"abstract":"<p><p>The minimally invasive and painless microneedle (MN) technology has become a promising platform for drug delivery and disease diagnosis. In this review, we first introduce the classification of MNs according to their sources and then summarize the preparation methods of MNs, including the stretching method, droplet-born air blowing, micromolding method, and 3D printing method. Subsequently, we also introduce how to prepare different types of MNs, such as solid, coated, hollow, dissolving, and frozen MNs, through material structure design. More importantly, the development of MNs in drug delivery, biosensing, wearable devices, cancer therapy and tissue regeneration in recent years has been reviewed. Finally, several significant challenges for further exploration in the field of MNs as well as perspectives and outlooks on future MN research, are also discussed in this review.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144616126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracking in vitro biodegradation dynamics in cartilage tissue engineering using dual-labelled hydrogel/scaffold composites.","authors":"Meenakshi Kamaraj, Lilith Mabel Caballero Aguilar, Serena Duchi, Stephanie E Doyle, Subha Narayan Rath, Simon E Moulton, Carmine Onofrillo","doi":"10.1088/1758-5090/adf3e7","DOIUrl":"https://doi.org/10.1088/1758-5090/adf3e7","url":null,"abstract":"<p><p>This study addresses the challenges of tracking cell-mediated biodegradation in cartilage tissue engineering. where hydrogels and scaffolds play a crucial role in providing structural support and promoting tissue regeneration. This research area has been rarely studied, offering potential insights into bridging the gap between in vitro and in vivo conditions for real-time monitoring of tissue regeneration alongside biodegradation. We developed dual-labelled hydrogel/scaffold composites for real-time monitoring of scaffold degradation in response to cell activity. Gelatin methacryloyl (GelMA) hydrogels are extensively explored for cartilage tissue engineering, albeit concerns remain regarding their mechanical properties under load-bearing conditions. To address this, a Hydrogel/Scaffold composite system was employed in this study, where a poly (ε-caprolactone) (PCL) hex prism edge structure acts as a scaffold supports of the cell-laden GelMA hydrogel. Fluorophore labelling of GelMA and PCL facilitated non-invasive monitoring of the Hydrogel/Scaffold composite biodegradation under cell proliferation conditions. Initially, the behaviour of fluorescent-tagged Hydrogel/Scaffold was examined under accelerated degradation conditions. Subsequently, human adipose-derived mesenchymal stem cells (hADSCs) loaded into fluorescent-labelled hydrogel/scaffolds were evaluated for their biocompatibility potential and chondrogenesis. Results demonstrated a correlation between the loss of fluorescence from Hydrogel/Scaffold degradation, accompanied by extracellular matrix accumulation. The fluorescently labelled hydrogel/scaffold holds promising application for cartilage tissue engineering, offering the capability to monitor biodegradation using high-throughput and contactless techniques.&#xD.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced combinatorial analysis of tumor cell-ECM interactions using design-of-experiment optimized microarrays.","authors":"Hannah Kimmel, Allison L Paxhia, Zahra Adamji, Gregory Underhill","doi":"10.1088/1758-5090/adf3e6","DOIUrl":"https://doi.org/10.1088/1758-5090/adf3e6","url":null,"abstract":"<p><p>The dysregulated and fibrotic tumor microenvironment of hepatocellular carcinoma (HCC) delays diagnosis and presents many complex signals that drive disease progression. To better recapitulate this microenvironment, we have enhanced our established protein microarray platform by integrating design of experiments (DoE) methodology with high-throughput cell microarray screening. This innovative approach systematically interrogates the intricate roles of matrix stiffness (spanning healthy and fibrotic conditions), extracellular matrix (ECM) composition, and protein concentration, while simultaneously examining their interdependent interactions. By leveraging DoE principles, we were able to explore 117 unique microenvironments on a single microscope slide, ultimately generating a comprehensive dataset of 234 different microenvironments without compromising statistical rigor. Our enhanced screening system enabled the identification of unique microenvironmental interactions critically significant in dictating cellular responses, including adhesion, survival, proliferation, epithelial-to-mesenchymal transition, and drug resistance markers. Utilizing advanced statistical techniques such as linear models and principal component analysis, we characterized phenotypic clusters defined by precise microenvironmental cues. This work presents a robust, high-throughput microarray screening system that comprehensively explores the contributions of 9 physiologically relevant extracellular matrix proteins and matrix stiffness in modulating cellular behavior and disease progression through a methodologically sophisticated and statistically sound approach.&#xD.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermally stable, photocrossinkable and biocompatible copolymers for melt electrowriting.","authors":"Sean O Mathew, Ronghui Qi, Brian G Amsden","doi":"10.1088/1758-5090/adef81","DOIUrl":"10.1088/1758-5090/adef81","url":null,"abstract":"<p><p>Melt electrowriting (MEW) is capable of generating highly defined microarchitectures suitable for tissue engineering applications. The main biodegradable polymer typically utilized for MEW processing, poly(<i>ϵ</i>-caprolactone), is prone to creep under dynamic loads and plasticization due to water absorption, making its use problematic for situations demanding dynamic loading in aqueous media. Photocrosslinking during processing can eliminate these problems while also allowing for manipulation of mechanical properties. However, photocrosslinking strategies utilized to date have either limited processing time or require prolonged UV irradiation. Herein we demonstrate the potential of a cyclic trimethylene carbonate monomer bearing a pendant coumarin moiety (MUM) for creating MEW processable copolymers that are thermally stable and photocrosslinkable. The MUM was copolymerized with caprolactone to form copolymers that were MEW processed into both linear and crimped fiber structures followed by long-wave UV photocrosslinking yielding high modulus scaffolds with very low sol content. The photocrosslinked scaffolds were also cytocompatible. The ability to copolymerize MUM with other cyclic lactone monomers allows for the generation of a variety of MEW processable polymers with tunable properties. Collectively, the findings demonstrate the potential of MUM containing copolymers for MEW generation of scaffolds for a range of tissue engineering applications.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering a microfluidic-assisted 3D bioprinting approach for the hierarchical control deposition and compartmentalisation of graded bioinks.","authors":"Federico Serpe, Lucia Iafrate, Marco Bastioli, Martina Marcotulli, Caterina Sanchini, Valeria De Turris, Michele D'Orazio, Biagio Palmisano, Arianna Mencattini, Eugenio Martinelli, Mara Riminucci, Carlo Massimo Casciola, Giancarlo Ruocco, Chiara Scognamiglio, Gianluca Cidonio","doi":"10.1088/1758-5090/adf35b","DOIUrl":"https://doi.org/10.1088/1758-5090/adf35b","url":null,"abstract":"<p><p>The advent of 3D bioprinting has revolutionised tissue engineering and regenerative medicine (TERM). Today, tissues of single cell type can be fabricated with extreme resolution and printing fidelity. However, the ultimate functionality of the desired tissue is limited, due to the absence of a multicellular population and diversity in micro-environment distribution. Currently, 3D bioprinting technologies are facing challenges in delivering multiple cells and biomaterials in a controlled fashion. The use of interchangeable syringe-based systems has often favoured the delamination between interfaces, greatly limiting the fabrication of interconnected tissue constructs. Microfluidic-assisted 3D bioprinting platforms have been found capable of rescuing the fabrication of tissue interfaces, but often fails to guarantee printing fidelity, cell density control and compartmentalisation. Herein, we present the convergence of microfluidic and 3D bioprinting platforms into a deposition system capable of harnessing a microfluidic printhead for the continuous rapid fabrication of interconnected functional tissues. The use of flow-focusing and passive mixer printhead modules allowed for the rapid and dynamic modulation of fibre diameter and material composition, respectively. Cells were compartmentalised into discrete three-dimensional layers with defined density patterns, confirming the punctual control of the presented microfluidic platform in arranging cells and materials in 3D. In ovo and in vivo studies demonstrated the seminal functionality of 3D bioprinted constructs with patterned vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1), respectively. This, in turn, facilitated the simulation of diverse cellular environments and proliferation pathways within a single construct, which is currently unachievable with conventional 3D bioprinting techniques, offering new opportunities for the fabrication of functionally graded systems and physiologically-relevant skeletal tissue substitutes.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near-infrared light and magnetic field dual-responsive 3D printed scaffolds for sequential treatment of infected bone defects.","authors":"Dapeng Zeng, Hao Wang, Zehao Yu, Xiaohan Mei, Boda Ying, Si Pu, Shibo Liu, Xiangjun Pan, Shicheng Zhou, Ruiyan Li, Yanguo Qin","doi":"10.1088/1758-5090/adebb3","DOIUrl":"10.1088/1758-5090/adebb3","url":null,"abstract":"<p><p>The treatment of infected bone defects remains a challenge due to the complex biological processes involved, including antibacterial, anti-inflammatory, angiogenesis and bone regeneration. Polyetherimide (PEI) has promising applications in orthopaedics, but its biological inertness limits its clinical efficacy. In this study, a smart near-infrared (NIR) light and magnetic field responsive 3D printed scaffold was developed by combining PEI and Fe₃O₄nanoparticles. Gelatin methacrylate hydrogel containing aloe-emodin (AE), a natural antimicrobial and antioxidant compound, was subsequently injected into the 3D printed scaffold to create the P-Fe₃O₄@GM-AE composite scaffold. This composite scaffold exhibited several key functionalities: Firstly, it effectively eliminated methicillin-resistant<i>Staphylococcus aureus</i>when exposed to NIR light, achieving an<i>in vivo</i>antimicrobial rate of 99.97 ± 0.1%. Secondly, it effectively removed reactive oxygen species and prevented the pro-inflammatory M1 polarization of macrophages in the infected bone defect microenvironment, creating favorable conditions for bone reconstruction. Moreover, during the reconstruction stage, the magnetic composite scaffold, when combined with a static magnetic field, promoted osteogenesis-angiogenesis coupling, thereby accelerating bone repair. Thus, this study provides new insights and methods for the sequential treatment of infected bone defects.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biohybrid microstructured hydrogels obtained via<i>in situ</i>extracellular matrix deposition and decellularization using supercritical CO₂.","authors":"Vanessa Morais Lima, Albane Carré, Emmanuelle Poque, Maria-Dimitra Chiotelli, Natan Wiele, Christelle Harscoat-Schiavo, Raphaëlle Savoire, Teresa Simon-Yarza","doi":"10.1088/1758-5090/adebb4","DOIUrl":"10.1088/1758-5090/adebb4","url":null,"abstract":"<p><p>In recent decades, our understanding of biomaterials has shifted from seeing them simply as physical supports for cells or drug delivery platforms to recognizing their active and dynamic role in tissue repair, guided by their physicochemical, mechanical, and biological properties. Biologically derived materials such as the decellularized extracellular matrix (dECM) offer the advantage of replicating the biomolecular cellular environment and have been proposed for tissue regeneration. However, their use as scaffolds is hindered by poor mechanical properties and limited tunability of physical features. Herein, we fabricated a bioinspired hybrid hydrogel by integrating a chemically cross-linked microporous polysaccharide scaffold with native ECM directly secreted by cells. First, the scaffold synthesis and culture conditions were optimized to enhance ECM deposition by fibroblasts. To obtain an acellular scaffold, decellularization using supercritical CO₂was performed and compared to a conventional method, demonstrating its superiority in ensuring efficient decellularization while preserving an enriched ECM lining the surface of the pores and preventing scaffold damage. The biohybrid hydrogel was characterized by a very low amount of DNA (<5 ng DNA mg^-1) and a network of highly interconnected pores covered by an abundant ECM including collagen I, collagen IV, fibronectin, elastin and laminin. This work presents a new versatile strategy that can be adapted to various tissues to engineer biomimetic microstructured materials overcoming the limitations associated with polymer-based and dECM-based strategies when used independently.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetically programmable wearable devices for precision physiological and molecular monitoring.","authors":"Jin He, Mengdie Fu, Wenyue An, Wenyi Xu, Jieruo Zhou, Yan Chen, Zichun Xia, Zhiwei Jiang, Guoli Yang","doi":"10.1088/1758-5090/adf25a","DOIUrl":"https://doi.org/10.1088/1758-5090/adf25a","url":null,"abstract":"<p><p>Wearable devices have emerged as powerful tools for continuous, real-time health monitoring, enabling the detection of biochemical markers in sweat, tears, saliva, and interstitial fluid. However, existing wearable materials are hindered by limited chemical functionality, static sensing capabilities, and insufficient adaptability to dynamic physiological conditions, which restrict their current impact in precision medicine. Recent advancements have focused on integrating genetic engineering and synthetic biology into wearable platforms, resulting in genetically programmable biointerfaces that enhance specificity, responsiveness, and functional versatility in clinical and personalized healthcare settings. Current applications of these bioengineered devices include real-time monitoring of pathogens, hormones, therapeutic drug levels, and physiological behaviors, offering superior precision and adaptability compared to traditional wearable technologies. This review highlights two key engineering approaches driving this field: genetically modified living cells and cell-free synthetic biology systems. While promising, several challenges still limit broader clinical adoption, including biosafety concerns, the instability of biological components, and translational hurdles. Addressing these challenges requires progress in biocompatibility, controlled gene expression, and durable wearable materials. Looking ahead, future research should aim to integrate these biointerfaces with implantable and smart therapeutic systems, develop autonomous biosensors with self-regulatory functions, and further expand their use in personalized medicine and real-time disease management. By bridging genetic programming with wearable diagnostics, these innovations are laying the groundwork for next-generation biohybrid systems designed to advance precision healthcare.&#xD.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}