Biofabrication最新文献

{"title":"Fabrication of multilayer heterogeneous cell assembly for pathophysiologically relevant 3D<i>in-vitro</i>IBD disease model for high throughput drug screening.","authors":"Mamta Kumari, Kamare Alam, Santanu Kaity, Sunil Kumar Sah, Velayutham Ravichandiran, Subhadeep Roy","doi":"10.1088/1758-5090/adc50e","DOIUrl":"10.1088/1758-5090/adc50e","url":null,"abstract":"<p><p>Regarding the approval of novel pharmaceuticals, the most common reason for failure is inadequate oral drug bioavailability. Owing to the complex physiological milieu of the human intestine, which is characterized by its varied composition, various functions, and one-of-a-kind dynamic conditions, it is difficult to reproduce the organ<i>in vitro</i>. Traditional monolayers in two dimensions, sophisticated three-dimensional systems, and developing fluid-dynamic platforms are examples of<i>in-vitro</i>intestinal models. Caco-2 cells have been the gold standard for studying drug permeability for over two decades, particularly for BCS Class II/III/IV drugs. Other intestinal<i>in vitro</i>models exist; however, pharmaceutical corporations and regulatory authorities use the Caco-2 cell line to predict human intestinal permeability. To predict oral drug absorption and study normal intestinal epithelial physiology, it is necessary to have advanced technologies capable of creating human intestinal epithelial cells (hIECs) with cellular variety and functions. There is a strong link between the permeability data obtained<i>in vitro</i>and the fractions absorbed by humans in complex multicellular models. However, although microphysiological systems accurately replicate physiological cues of the digestive tract, they still require standardization. We critically reviewed a step towards tissue-created 3D intestinal organoids and 3D heterocellular multicompartmental models without compromising cellular variety and function. To bridge the gap between 2D and 3D intestinal culture models, a physiologically appropriate hIEC model provides a novel platform for patient-specific testing and translational applications. A comprehensive understanding of numerous 3D<i>in-vitro</i>models of inflammatory bowel disease has been discussed. Additionally, this review will provide insights into the benefits and limitations of these models and their relevance in understanding intestinal physiology and accelerating drug discovery through high-throughput screening.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: Toward a neurospheroid niche model: optimizing embedded 3D bioprinting for fabrication of neurospheroid brain-like co-culture constructs (2021<i>Biofabrication</i>13 015014).","authors":"Yi-Chen Ethan Li, Yasamin A Jodat, Roya Samanipour, Giulio Zorzi, Kai Zhu, Minoru Hirano, Karen Chang, Adnan Arnaout, Shabir Hassan, Navneet Matharu, Ali Khademhosseini, Mina Hoorfar, Su Ryon Shin","doi":"10.1088/1758-5090/adb74a","DOIUrl":"https://doi.org/10.1088/1758-5090/adb74a","url":null,"abstract":"","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":"17 2","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of highly vascularized hepatic spheroids of primary hepatocytes via pro-angiogenic strategy in vitro.","authors":"Yen-Hsiang Huang, Masafumi Watanabe, Tadahiro Yamashita, Ryo Sudo","doi":"10.1088/1758-5090/adc8d4","DOIUrl":"https://doi.org/10.1088/1758-5090/adc8d4","url":null,"abstract":"<p><p>Primary hepatocytes are widely recognized for their ability to accurately represent the in vivo hepatocyte phenotype. However, traditional avascular primary hepatocyte culture models are limited by inadequate mass transfer, which leads to a rapid decline in hepatocyte function and survival. To address these challenges, vascularization of hepatic spheroids is crucial for enhancing oxygen and nutrient supply, thereby enabling the construction of larger and more complex hepatic tissues in vitro. In this study, we achieved vascularization of hepatic spheroids containing freshly isolated primary hepatocytes by incorporating fibroblasts as a source of paracrine factors to induce angiogenesis. Multicellular spheroids composed of primary hepatocytes and fibroblasts were formed in non-adhesive concave wells, and one of the spheroids was subsequently embedded in a fibrin-collagen hydrogel within a microfluidic device. Endothelial cells were then seeded onto adjacent microfluidic channels. They formed microvascular networks that extended toward and penetrated the hepatic spheroid. The vascularized hepatic spheroid closely mimicked hepatic sinusoids, with hepatocytes in close contact with microvessels. Moreover, the vascularized spheroid exhibited significantly enhanced hepatic function, specifically albumin secretion. Our findings provide insights into the establishment of highly vascularized hepatic spheroids in vitro, which is crucial for constructing scalable hepatic tissues in the context of biofabrication.&#xD.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellnet technology to generate 3D, functional, single-cell networks in custom architectures within collagen.","authors":"Arun Poudel, Puskal Kunwar, Ujjwal Aryal, Anna-Blessing Merife, Pranav Soman","doi":"10.1088/1758-5090/adc48f","DOIUrl":"10.1088/1758-5090/adc48f","url":null,"abstract":"<p><p>Cells possess the remarkable ability to generate tissue-specific 3D interconnected networks and respond to a wide range of stimuli. Understanding the link between the spatial arrangement of individual cells and their networks' emergent properties is necessary for the discovery of both fundamental biology as well as applied therapeutics. However, current methods spanning from lithography to 3D photo-patterning to acoustofluidic devices are unable to generate interconnected and organized single cell 3D networks within native extracellular matrix (ECM). To address this challenge, we report a novel technology coined as Cellnet. This involves the use of natural collagen crosslinked within three-chambered microfluidic chips followed by femtosecond laser-assisted cavitation to generate user-defined 3D microchannel networks. Model cells, seeded within side chamber of the chip, migrate within microchannel networks within hours, self-organize and form viable, interconnected, 3D single-cell networks in custom architectures such as square grid, concentric circle, parallel lines, and spiral patterns. Heterotypic Cellnets can also be generated by seeding multiple cell types in side-chambers of the chip. The functionality of cell networks can be studied by monitoring the real-time calcium signaling response of individual cells and signal propagation within Cellnets when subjected to flow stimulus alone or a sequential combination of flow and biochemical stimuli. Furthermore, user-defined disrupted Cellnets can be generated by lethally injuring target cells within the 3D network and analyzing the changes in their signaling dynamics. As compared to the current self-assembly based methods that exhibit high variability and poor reproducibility, Cellnets can generate organized 3D single-cell networks and their real-time signaling responses to a range of stimuli can be accurately captured using simple cell seeding and easy-to-handle microfluidic chips. Cellnet technology, agnostic of cell types, ECM formulations, 3D cell-connectivity designs, or location and timing of network disruptions, could pave the way to address a range of fundamental and applied bioscience applications.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combinatorial strategy for engineering cartilage and bone microtissues using microfluidic cell-laden microgels.","authors":"Suntae Kim, Siyuan Li, Seung Yeop Baek, Chaenyung Cha, Sang Jin Lee","doi":"10.1088/1758-5090/adc840","DOIUrl":"https://doi.org/10.1088/1758-5090/adc840","url":null,"abstract":"<p><p>Osteochondral defects refer to localized injuries affecting both the avascular cartilage and subchondral bone. Current treatments, such as transplantation or microfracture surgery, are hindered by limitations like donor availability and the formation of small, rigid fibrocartilage. Tissue engineering presents a promising alternative, yet challenges arise from limited oxygen and nutrient supply when fabricating human-scale tissue constructs. To address this, we propose assembling engineered micro-scale tissue constructs as building blocks for human-scale constructs. In this study, we aimed to develop bone and cartilage microtissues as building blocks for osteochondral tissue engineering. We fabricated placental stem cell (PSC)-laden microgels, inducing differentiation into osteogenic and chondrogenic microtissues. Utilizing a microfluidics chip platform, these microgels comprised a cell-laden core containing bone-specific and cartilage-specific growth factor-mimetic peptides, respectively, along with an acellular hydrogel shell. Additionally, we investigated the effect of culture conditions on microtissue formation, testing dynamic and static conditions. Results revealed over 85% cell viability within the microgels over 7 days of continuous growth. Under static conditions, approximately 60% of cells migrated from the core to the periphery, while dynamic conditions exhibited evenly distributed cells. Within 4 weeks of differentiation, growth factor-mimetic peptides accelerated PSC differentiation into bone and cartilage microtissues. These findings suggest the potential clinical applicability of our approach in treating osteochondral defects.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A hollow fiber membrane-based liver organoid-on-a-chip model for examining drug metabolism and transport.","authors":"Adam Myszczyszyn, Anna Muench, Vivian Lehmann, Theo Sinnige, Frank G van Steenbeek, Manon Bouwmeester, Roos-Anne Samsom, Marit Keuper-Navis, Thomas K van der Made, Daniel Kogan, Sarah Braem, Luc J W van der Laan, Hossein Eslami Amirabadi, Evita van de Steeg, Rosalinde Masereeuw, Bart Spee","doi":"10.1088/1758-5090/adc3ce","DOIUrl":"10.1088/1758-5090/adc3ce","url":null,"abstract":"<p><p>Liver-on-a-chip models predictive for both metabolism, and blood and canalicular transport of drug candidates in humans are lacking. Here, we established a bioengineered and 3Rs-complied (animal component-free) hepatocyte-like millifluidic system based on 3D hollow fiber membranes (HFMs), recombinant human laminin 332 coating and adult human stem cell-derived organoids. Organoid fragments formed polarized and tight monolayers on HFMs with improved hepatocyte-like maturation, as compared to standard 3D organoid cultures in Matrigel from matched donors. Gene expression profiling and immunofluorescence revealed that hepatocyte-like monolayers expressed a broad panel of phase I (e.g. CYP3A4, CYP2D6, CYP2C9) and II (e.g. UGTs, SULTs) drug-metabolizing enzymes and drug transporters (e.g. MDR1, MRP3, OATP1B3). Moreover, statically cultured monolayers displayed phase I and II metabolism of a cocktail of six relevant compounds, including midazolam and 7-hydroxycoumarin. We also demonstrated the disposition of midazolam in the basal/blood-like circulation and apical/canalicular-like compartment of the millifluidic chip. Finally, we studied the bioavailability of midazolam and coumarin on-a-chip in combination with a small intestine-like system. In conclusion, we generated a proof-of-concept liver organoid-on-a-chip model for examining metabolism and transport of drugs, which can be further developed to predict pharmacokinetics' (PK)/absorption, distribution, metabolism and excretion (ADME) profiles in humans.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in Selective Laser Melting (SLM) of titanium alloy scaffolds for bone tissue engineering.","authors":"Kelun Yan, Nor Hasrul Akhmal Ngadiman, Muhammad Zameri Mat Saman, Nur Syahirah Mustafa","doi":"10.1088/1758-5090/adc6c0","DOIUrl":"https://doi.org/10.1088/1758-5090/adc6c0","url":null,"abstract":"<p><p>Selective Laser Melting (SLM) has emerged as a transformative technology in bone tissue engineering, particularly for fabricating porous scaffolds from titanium alloys. These scaffolds offer a promising solution for treating critical-sized bone defects, providing mechanical support while promoting bone regeneration. A comprehensive review on recent advancements of SLM is provided by presenting a detailed analysis of cutting-edge research in the application of SLM for titanium alloy scaffold production. Key areas explored include structural designs like Triply Periodic Minimal Surfaces (TPMS), material and process parameters optimization to enhance scaffold properties such as porosity, mechanical strength, and biocompatibility. Furthermore, the review emphasizes recent innovations in surface modification techniques which improve bioactivity and osseointegration to enable scaffolds to mimic the host tissues. In addition, this review provides essential insights in related to the potential of SLM to be adopted in producing personalized and high-performance medical implants. By synthesizing the latest trends and identifying key areas for future research, this paper aims to serve as a vital resource for the advancement and usage of SLM-fabricated scaffolds in clinical applications. The findings underscore the importance of continued innovation in this field, which has the potential to significantly improve patient outcomes in orthopaedics and beyond.&#xD.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering strategies for the construction of oriented and functional skeletal muscle tissues.","authors":"Tingting Fan, Minxuan Jia, Heng Liu, Zili Gao, Wenhui Huang, Wenli Liu, Qi Gu","doi":"10.1088/1758-5090/adbfc2","DOIUrl":"10.1088/1758-5090/adbfc2","url":null,"abstract":"<p><p>The growth and formation of tissues, such as skeletal muscle, involve a complex interplay of spatiotemporal events, including cell migration, orientation, proliferation, and differentiation. With the continuous advancement of<i>in vitro</i>construction techniques, many studies have contributed to skeletal muscle tissue engineering (STME). This review summarizes recent advances in the ordered construction of skeletal muscle tissues, and evaluates the impact of engineering strategies on cell behavior and maturation, including biomaterials, manufacturing methods and training means. Biomaterials are used as scaffolds to provide a good microenvironment for myoblasts, manufacturing methods to guide the alignment of myoblasts through construction techniques, and external stimulation to further promote the myoblast orientation and maturation after construction, resulting in oriented and functional skeletal muscle tissues. Subsequently, we critically examine recent advancements in engineered composite skeletal muscle constructs, with particular emphasis on essential functionalization strategies including skeletal muscle vascularization, innervation and others. Concurrently, we evaluate emerging applications of STME in diverse translational areas such as volumetric muscle loss treatment, muscle-related disease models, drug screening, biohybrid robots, and cultured meat. Finally, future perspectives are proposed to provide guidance for rational design based on engineering strategies in STME.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-based multi-material bioprinting of vascularised adipose tissue for breast fatty tissue engineering.","authors":"Nina Hedemann, Alexander Thomas, Nils Tribian, Anna-Klara Amler, Sandra Krüger, David Holthaus, Patricia Huebbe, Inken Flörkemeier, Jörg Weimer, Nicolai Maass, Lutz Kloke, Dirk Bauerschlag, Marion Tina van Mackelenbergh","doi":"10.1088/1758-5090/adb890","DOIUrl":"10.1088/1758-5090/adb890","url":null,"abstract":"<p><p>Reconstructive surgery following breast cancer ablation is a surgical gold standard, but current options comprising autologous fatty tissue transfer and artificial soft tissue implants are inferior. With the advent of powerful biofabrication technologies, researchers for the first time have the tools to engineer life-like tissues with the ultimate goal of clinical application. Here, we apply multi-material stereolithographic bioprinting together with a novel sacrificial biomaterial system to engineer complex fatty tissue constructs. Biomaterials, cellular composition and cultivation conditions of these constructs were designed to enable<i>in vitro</i>creation of vascularised fatty tissue. Cells within the constructs showed an overall good survival (>93%), indicated by live-dead cell staining, over the entire cultivation period of 27 d. Adipose-derived stem cells were successfully differentiated<i>in situ</i>, forming fat vesicles and expressing adipocyte markers PPARγ, FAPB4 and S100B. Additionally, secretion of adipokines leptin and adiponectin into culture supernatants increased significantly. Endothelial cells vascularised the constructs, creating macro- and microvascular structures within the printed channels and extending beyond with culture time. Moreover, cells invaded into the surrounding hydrogel. The engineered fatty tissue constructs could serve as a base to develop patient-specific tissue building blocks with the final goal to achieve an all-natural reconstruction of the breast.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D bioprinting technology for modeling vascular diseases and its application.","authors":"Ju-El Kim, Gun-Jae Jeong, Young Min Yoo, Suk Ho Bhang, Jae Hoon Kim, Young Min Shin, Kyung Hyun Yoo, Byung-Chul Lee, Wooyeol Baek, Dong Nyoung Heo, Rosaire Mongrain, Jung Bok Lee, Jeong-Kee Yoon","doi":"10.1088/1758-5090/adc03a","DOIUrl":"10.1088/1758-5090/adc03a","url":null,"abstract":"<p><p><i>In vitro</i>modeling of vascular diseases provides a useful platform for drug screening and mechanistic studies, by recapitulating the essential structures and physiological characteristics of the native tissue. Bioprinting is an emerging technique that offers high-resolution 3D capabilities, which have recently been employed in the modeling of various tissues and associated diseases. Blood vessels are composed of multiple layers of distinct cell types, and experience different mechanical conditions depending on the vessel type. The intimal layer, in particular, is directly exposed to such hemodynamic conditions inducing shear stress, which in turn influence vascular physiology. 3D bioprinting techniques have addressed the structural limitations of the previous vascular models, by incorporating supporting cells such as smooth muscle cells, geometrical properties such as dilation, curvature, or branching, or mechanical stimulation such as shear stress and pulsatile pressure. This paper presents a review of the physiology of blood vessels along with the pathophysiology of the target diseases including atherosclerosis, thrombosis, aneurysms, and tumor angiogenesis. Additionally, it discusses recent advances in fabricating<i>in vitro</i>3D vascular disease models utilizing bioprinting techniques, while addressing the current challenges and future perspectives for the potential clinical translation into therapeutic interventions.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}