Biofabrication最新文献

{"title":"Efficient wet-spinning of pre-aligned microtissues for 3D bioprinting complex tissue alignment.","authors":"Caleb D Vogt, Joseph R Broomhead, Kyle Y Kunisaki, Johanna Margaret Teegarden, Kallie L Frett, Kyleigh Q Pacello, Anthony H Vitale, Angela Panoskaltsis-Mortari","doi":"10.1088/1758-5090/add37f","DOIUrl":"10.1088/1758-5090/add37f","url":null,"abstract":"<p><p>Engineering functional smooth muscle tissues requires precise control of cellular alignment, particularly in complex anatomical regions such as the gastroesophageal junction (GEJ). We present a scalable wet-spinning approach for generating pre-aligned microtissues (PAMs) from immortalized human esophageal smooth muscle cells embedded in a collagen-alginate core-shell fiber. After maturation, fibers were sectioned into uniform PAMs with preserved alignment and high cell viability. Immunofluorescence and gene expression analyses confirmed the expression of key contractile markers. PAMs were incorporated into a gelatin-methacryloyl bioink and 3D bioprinted to demonstrate alignment along the extrusion path. This method does not require specialized culture platforms and enables efficient production of aligned microtissues for bioprinting. It offers a promising strategy for fabricating anisotropic tissues and may facilitate the reconstruction of complex muscle structures such as the GEJ.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12083473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Additive manufacturing of silicon nitride fiber-reinforced polyetheretherketone composites with enhanced mechanical strength and multifunctional bioactivity for load-bearing bone defect repair.","authors":"Shengxin Zeng, Haozheng Li, Panpan Hu, Zihe Li, Zhengguang Wang, Jiedong Wang, Jiasheng Chen, Shouzhan Wang, Gong Wang, Wei Zhao, Feng Wei","doi":"10.1088/1758-5090/add9d3","DOIUrl":"https://doi.org/10.1088/1758-5090/add9d3","url":null,"abstract":"<p><p>Polyetheretherketone (PEEK) is increasingly applied in bone defect repair due to its excellent biocompatibility and absence of artifact formation. However, the bio-inertness and inadequate mechanical properties of untreated PEEK remain significant challenges for PEEK-based implants. Hence, this study prepares a series of silicon nitride (Si3N4) fiber-reinforced PEEK composite porous scaffolds using twin-screw melt mixing-extrusion and material extrusion 3D printing. Comprehensive evaluations assess the mechanical properties, biocompatibility, osteogenic differentiation, angiogenesis activities, and antibacterial performances of various composites. Characterization results show that Si3N4 fiber-reinforced PEEK composites exhibit excellent printability, with well-oriented Si3N4 fibers uniformly distributed throughout the matrix. Furthermore, compared to non-reinforced PEEK, the addition of 8% Si₃N₄ fibers enhanced Young's modulus by 52.2% (6.36 GPa). Additionally, both in vitro and in vivo results indicate that all composite scaffolds exhibit excellent biocompatibility. Notably, the 8% Si₃N₄ fiber-reinforced PEEK composite demonstrated optimal multifunctional performance in osteogenic induction, angiogenic capacity, and antibacterial efficacy, significantly outperforming other experimental groups. In conclusion, this study offers a solution for enhancing the mechanical, anti-infective, and osseointegrative properties of PEEK, demonstrating its great potential for expanding the application of non-metallic orthopedic implants in bone defect repair.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional nanoplatform based on polyethylene glycol-folic acid modified UiO-66 (Zr) as drug delivery platform for enhanced therapy of cancer.","authors":"Mengyuan Li, Jiaming Ge, Jingwen Yao, Yuanhao Zhang, Lin Ma, Zheng Li, Xiangli Han, Ming Liu, Fei Tian, Jing Zhao","doi":"10.1088/1758-5090/add9d2","DOIUrl":"https://doi.org/10.1088/1758-5090/add9d2","url":null,"abstract":"<p><p>Oral squamous cell carcinoma (OSCC) is the most common malignant tumor in the head and neck. Due to low bioavailability and passive targetability of anticancer drugs show great limitations in cancer therapy, the treatment of OSCC faces major challenges. Folic acid (FA) targeting can deliver anticancer drugs efficiently into the tumor environment, further enhance the anti-cancer efficacy. Herein, the nanoplatform based on UiO-66 that encapsulated with an effective FA targeting ligands and the pH-responsive polyethylene glycol (PEG) layer for the targeted delivery of berberine (Ber) is constructed for fighting against OSCC. The FA modification and controlled pH-responsiveness enable the targeted delivery of UiO-66/PEG-FA, which promotes the release of Ber and increases the cumulative intracellular Ber concentration, which both promote consumption of glutathione (GSH) and induced generation of reactive oxygen species (ROS), further stimulate the secretion of inflammatory factors (TNF-α and IL-1β). A comprehensive evaluation of in vitro and in vivo experiments show that UiO-66@Ber/PEG-FA promote autophagy and apoptosis of tumor cells by regulating the expression of Beclin-1, ATG13, BAX and Bcl-2, and effectively inhibit tumor growth. Overall, UiO-66@Ber/PEG-FA exhibit superior pH-responsiveness and targeted therapeutic efficiencies in vitro and vivo, it can serve as an approach for OSCC therapy.&#xD.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High resolution melt electro-written scaffolds promote alignment of human skeletal muscle cells.","authors":"Finn Snow, Cathal O'Connell, Aaron Elbourne, Magdalena Kita, Peiqi Yang, Richard Williams, Simon E Moulton, Elena Pirogova, Robert Michail Ivan Kapsa, Anita Quigley","doi":"10.1088/1758-5090/add960","DOIUrl":"https://doi.org/10.1088/1758-5090/add960","url":null,"abstract":"<p><p>Advanced tissue engineering strategies are vital to address challenging musculoskeletal conditions, such &#xD;as volumetric muscle loss. These disorders impose a considerable economic burden and affect &#xD;individuals' quality of life, highlighting the need for innovative treatments, such as tissue engineering, &#xD;to address these challenges. Here, we examine how scaffold fibre orientation influences mechanical &#xD;properties and cellular behaviour by utilising Melt Electrowriting (MEW) as a high-resolution 3D &#xD;printing technique that combines aspects of electrospinning and melt based polymer deposition. In this &#xD;work, we investigated the effects of fibre orientation in MEW scaffolds, and its effect on the scaffold &#xD;mechanical properties as well as cell orientation and alignment. MEW scaffolds were mechanically &#xD;characterized through uniaxial strain testing to determine critical parameters, including strain at failure &#xD;(SAF), ultimate tensile strength (UTS), Young's modulus (E), fatigue rate, recovery time, and yield &#xD;strain. These mechanical properties were analysed to define an optimal strain regime for transitioning &#xD;from static to dynamic culture conditions under muscle-like cyclic loading, relevant to muscle's &#xD;viscoelastic behaviour. In parallel, static cultures of human skeletal myotubes and normal human dermal &#xD;fibroblasts were grown on MEW scaffolds, with varying architectures, to study the effects of fibre aspect &#xD;ratio on cell alignment. Cell alignment was visualized using DAPI/phalloidin staining and quantified &#xD;with the ImageJ directionality plugin, enabling a systematic comparison of scaffold designs. This &#xD;approach evaluates the potential of supportive scaffold architectures to promote aligned cell growth, &#xD;offering insights into designing effective scaffolds for tissue regeneration.&#xD.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in microfluidic biofabrication technology for bone metastasis modeling.","authors":"Mehdi Khanmohammadi, Nima Ahmadkhani, Marina Volpi, Khadijeh Khederlou, Alankrita Uppal, Mahdis Hosseini, Y Shrike Zhang, Wojciech Święszkowski","doi":"10.1088/1758-5090/add95f","DOIUrl":"https://doi.org/10.1088/1758-5090/add95f","url":null,"abstract":"<p><p>Studying bone metastasis in in vitro models is essential for understanding the mechanisms driving this process, developing effective therapeutic strategies, and evaluating potential treatments for metastatic cancer patients. To this end, traditional two-dimensional (2D) cell culture models fail to replicate the native three-dimensional (3D) tissue microenvironment, resulting in significant disparities in biologically relevant behaviors and drug responses. The shift from 2D to 3D cell culture techniques represents an important step toward creating more biomimetic bone metastasis models. These systems more effectively emulate and replicate the complex interactions between cancer cells and bone tissue, including essential cell-cell and cell-extracellular matrix interactions, as well as in vivo biomechanical cues. The development and application of microfluidic-based 3D cancer models, incorporating diverse shapes, architectures, and modular structures such as organ-on-chip platforms, enable comprehensive screening and exploration of cellular interplay, the dissection of signaling pathways, and the resolution of limitations associated with traditional models. This review highlights recent advancements in microfluidic-based 3D bone metastasis models and examines innovative applications of this technology. These include hydrogel-based spherical and filaments biofabrication approaches, 2D and 3D tumor on-a- chips, and drug screening techniques such as concentration gradient generator-based, microdroplet-based, and microarray-based chips, as well as tumor tissue chips. Additionally, we discuss the benefits and limitations of these approaches in treating bone metastases and propose future directions for advancing microfluidic platforms in drug discovery and this research field.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of the exosomal protein SERPINA11 as a potential atherosclerosis marker via bioprinted scaffold.","authors":"Kyung-Seob Kim, Seung-Cheol Choi, Ji-Min Noh, Myeong-Hwa Song, Seongmin Jun, Ji Eun Na, Im Joo Rhyu, Do-Sun Lim","doi":"10.1088/1758-5090/add8bf","DOIUrl":"https://doi.org/10.1088/1758-5090/add8bf","url":null,"abstract":"<p><p>Existing animal and human cell models have limitations in terms of heterogeneous differences or difficulties in sufficiently reproducing arterial structures and complex cell-cell interactions. The discovery of exosome-derived biomarkers using a 3D bioprinted atherosclerosis model provides a noninvasive and stable detection method and is expected to contribute to the development of early diagnosis and personalized treatment. To contribute to the discovery of exosome-derived biomarkers related to the early diagnosis and prognosis of cardiovascular diseases using a 3D bioprinted atherosclerosis model, we reproduced an arterial environment using 3D bioprinting composed of a biocompatible extracellular matrix (bioink) and various human cells in vitro. The 3D bioprintedatherosclerosis model composed of inflammatory macrophages, coronary artery smooth muscle cells, coronary artery endothelial cells, and collagen methacryloyl (ColMA) hydrogel was treated with LDL to induce atherosclerosis, and the atherosclerosis model was classified into Baseline (BL), Early Atherosclerosis (EA; Early Athero), and Late Atherosclerosis (LA; Late Athero) groups. The secreted exosomes were isolated according to the time period, and a characterization analysis was conducted to confirm the purity of the isolated exosomes. We evaluated the isolated exosomes qualitatively and quantitatively. Isolated exosomes were analyzed using proteomics and miRNA sequencing to verify whether the bioprinted atherosclerosis model induced atherosclerosis, and a novel early atherosclerosis biomarker, SERPINA11, was discovered. In conclusion, we verified that the bioprinted atherosclerosis model induced atherosclerosis and that the novel biomarker set of exosomal miRNAs (hsa-miR-143-5p and hsa-miR-6879-5p) expressed in early atherosclerosis and proteins (SERPINA11, AHSG, and F2) might be clinically useful in early diagnosis and prognosis.&#xD.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprinted M2 macrophage-derived extracellular vesicle mimics attenuate foreign body reaction and enhance vascularized tissue regeneration.","authors":"Chao Zhang, Ze Fu, Qinghua Liu, Xu Guo, Zhao Li, Wei Song, Yi Kong, Jinpeng Du, Yanlin Su, Bingyang Yu, Yue Kong, Feng Tian, Xiaobing Fu, Xiaohui Du, Sha Huang","doi":"10.1088/1758-5090/add49f","DOIUrl":"https://doi.org/10.1088/1758-5090/add49f","url":null,"abstract":"<p><p>Foreign body reaction (FBR) and insufficient vascularization greatly hinder the integration of 3D-bioprinted tissue substitutes with host tissues. Previous studies have shown that these problems are exacerbated by the stiffness of the 3D-bioprinted constructions, which is highly associated with the abnormal polarization of macrophages. Therefore, we developed an engineering strategy using membrane extrusion to prepare macrophage-derived extracellular vesicle mimics (EVMs). The EVMs derived from M1 and M2 macrophages (M1-EVMs and M2-EVMs) were rich in functional proteins. In the 2D environment, M1-EVMs promoted the fibrotic phenotype of fibroblasts, vascularization, and the M1 polarization of macrophages. In contrast, M2-EVMs effectively avoided the fibrotic trend, showed stronger angiogenic capabilities, and prevented excessive M1 polarization, demonstrating their potential to inhibit FBR and promote neovascularization. After bioprinting the EVMs loaded by gelatin-alginate bioink, the basic physical properties of the bioink were not significantly affected, and the biological functions of EVMs remain stable, indicating their potential as bioink additives. In the subcutaneous implantation model, unlike the FBR-aggravating effects of M1-EVMs, 3D-bioprinted M2-EVMs successfully reduced the immune response, prevented fibrous capsule formation, and increased vascular density. When applied to skin wound treatment, 3D-bioprinted M2-EVMs not only inhibited inflammatory levels but also exhibited pleiotropic pro-regenerative effects, effectively promoting vascularization, re-epithelialization, and appendage regeneration. As an innovative additive for bioinks, M2-EVMs present a promising approach to enhance the survival of bioengineered tissues and can further serve as a targeted drug loading system, promoting the development of regenerative medicine and improving clinical outcomes.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":"17 3","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-tiny-scale technology for engineering human ear therapeutics.","authors":"Harshita Sharma, Woochan Kim, Sejong Oh, Dream Kim, Shinyull Lee, Sangbae Park, Jooseon Oh, Sunho Park, Jangho Kim","doi":"10.1088/1758-5090/add210","DOIUrl":"https://doi.org/10.1088/1758-5090/add210","url":null,"abstract":"<p><p>Ultra-tiny-scale technology representing engineered micro- and nano-scale materials has gained considerable attention for a wide range of applications, including hearing restoration. The advent of hearing loss and its recovery has been the topic of intense discussion since many decades. Although conventional treatments partially support hearing recovery, they present certain limitations such as subsequent immune response and donor site morbidity leading to even worsened sensory disturbances. Microscale- and nanoscale-based approaches such as tissue engineering, nanoparticle-assisted drug delivery systems, and micro/nanofabrication-aided auditory stimulations have been shown to play an efficient role in recovery from hearing disorders. In particular, the introduction of different biomaterials and biopolymers (natural and synthetic) with influential topographical cues and excellent biocompatibility has been found to conveniently bypass previous challenges posed by rigid human ear structures and provided a new path for improved and advanced hearing-recovery approaches. This review is focused on the development of micro/nanoengineering-based hearing recovery therapeutics and their significant impact on the future of hearing research. It discusses the physiological functions associated with the human ear and the mechanism underlying distinct hearing loss disorders as well as highlights various engineered ultra-tiny-scale-assisted strategies for developing advanced hearing therapeutics. Finally, we deliberate on commercialization aspect and future perspectives of implementing micro/nanotechnologies for hearing restoration platforms.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":"17 3","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a bioreactor and volumetric bioprinting protocol to enable perfused culture of biofabricated human epithelial mammary ducts and endothelial constructs.","authors":"Maj-Britt Buchholz, Paulina Nunez Bernal, Nils Bessler, Camille Bonhomme, Riccardo Levato, Anne Rios","doi":"10.1088/1758-5090/add20f","DOIUrl":"https://doi.org/10.1088/1758-5090/add20f","url":null,"abstract":"<p><p>Tissue function depends on the 3D spatial organization of cells, extracellular matrix components, as well as dynamic nutrient gradients and mechanical forces. Advances in biofabrication technologies have enabled the creation of increasingly sophisticated tissue models, but achieving native-like tissue maturation post-fabrication remains a challenge. The development of bioreactors and microfluidic systems capable of introducing dynamic culture platforms and controlled mechanical and biochemical stimulation for biofabricated tissue analogues is therefore imperative to address this. In this technical note, we introduce a multi-step pipeline to fabricate, seed and perfuse geometrically complex hydrogel constructs with quality control protocols through the computational analysis of confocal multispectral 3D imaging data for each step of the process. Employing ultra-fast volumetric bioprinting, chips with tunable channel architectures were fabricated. Furthermore, an autoclavable and transparent perfusion bioreactor inspired by open-source designs was developed to enable controlled, long-term perfusion (up to 28 days) and real-time monitoring of cell behavior. As proof-of-concept, employing this pipeline, we fabricated a human mammary ductal model and an endothelialized vessel on-a-chip, demonstrating the compatibility of the platform with epithelial and endothelial cell lines, and investigated the effect of dynamic culture on tissue-specific cell organization. Dynamic perfusion underlined the influence of mechanical stimulation on cell organization and maturation. Various chip architectures, capable of recapitulating tissue-specific features (<i>i.e.</i>lobules) were printed, enabling the mono- and co-culture of human mammary epithelial and endothelial cells. Our pipeline, with the accompanying protocols and analysis scripts presented here, provide the potential to be applied for the dynamic culture of a wide range of tissues.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":"17 3","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetically modified cell membrane proteins in tissue engineering and regenerative medicine.","authors":"Yilin Bao, Yue Hu, Mengxuan Hao, Qinmeng Zhang, Guoli Yang, Zhiwei Jiang","doi":"10.1088/1758-5090/add625","DOIUrl":"https://doi.org/10.1088/1758-5090/add625","url":null,"abstract":"<p><p>Genetically modified cell membrane proteins can effectively regulate cell proliferation and differentiation, while also integrating novel biomaterials. As a promising biomedical tool, this technology has broad applications in tissue engineering and regenerative medicine. Both viral and non-viral gene transfection methods have been employed to create genetically modified cell membrane proteins. Numerous studies have demonstrated the significant efficacy of genetically modified cell membrane proteins in promoting bone regeneration, treating cardiovascular diseases, aiding lung injury recovery, advancing immunotherapy, and in applications involving engineered cell membrane sheets and cell spheroids. However, this technology faces several limitations, including biosafety and ethical concerns associated with genetic modification. This article summarizes recent advances in genetically modified cell membrane proteins, detailing their preparation, applications, limitations, and future directions.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}