Biofabrication最新文献

{"title":"Versatile polyacrylamide/gum hybrid hydrogels for tunable mechanics and high-fidelity 3D extrusion printing.","authors":"Mehwish Yousaf, Hazal Kutluk, Leon Balters, Stephan Reichl, Iordania Constantinou","doi":"10.1088/1758-5090/aeb62e","DOIUrl":"https://doi.org/10.1088/1758-5090/aeb62e","url":null,"abstract":"<p><p>Polyacrylamide (PAM) hydrogels are widely used in biological research for their well-established and tunable mechanical properties, which can closely match native tissue stiffness, making them attractive for mechanobiology, biosensing and tissue engineering. However, extrusion-based 3D printing of PAM remains challenging due to the inherently low viscosity of precursor solutions. Existing strategies reach printable viscosities with rheology modifiers such as Laponite clays or protein-based microgels, but these additives strongly couple printability to final stiffness, substantially increasing the elastic modulus and compromising the soft, tunable character that makes PAM valuable. Here, we present a novel yet accessible one-pot strategy for extrusion-printable PAM hydrogels, using natural polysaccharide gums as a distinct class of rheology modifier. Unlike conventional additives, gums confer the shear-thinning and viscoelastic structure required for extrusion at low concentrations (2-4 w/v %), while preserving the established AM/BIS-stiffness relationship and allowing modulus to be tuned independently through monomer concentration. Tragacanth, xanthan, and guar gums were systematically evaluated across concentrations for their effects on ink rheology, print fidelity, and mechanics. High-fidelity printing was achieved at concentrations as low as 2 w/v % for guar and 3 w/v % for tragacanth and xanthan. Mechanical characterization reveals soft, tunable properties, with elastic moduli from 5 to 58 kPa across gum type and concentration, spanning a broad range of soft tissue stiffnesses. Guar and tragacanth in particular produced moduli statistically comparable to pure PAM, confirming minimal impact on network mechanics. Preliminary cytocompatibility assays confirm these hybrid hydrogels support MDCK I cell viability. We further demonstrate core-shell extrusion printing of PAM/gum hydrogels for the first time, using a sacrificial core to generate hollow, perfusable channels down to ~200 µm in diameter. Together, these results establish PAM/gum hydrogels as a printable, mechanically tunable ink platform, laying a materials-processing foundation for architecturally complex, channeled PAM-based constructs.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149222948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Granular hydrogel bioink for macroporous pancreatic spheroid scaffolds.","authors":"Theresa Seah, Jingyi Zhang, Juin Ting Chen, Joyce Tay Jia Xin, Wan Ling Wong, Adrian Teo, Cyrus Beh","doi":"10.1088/1758-5090/aeb62d","DOIUrl":"https://doi.org/10.1088/1758-5090/aeb62d","url":null,"abstract":"<p><p>Cells positioned more than several hundred microns away from a vascular network experience insufficient oxygen and nutrient supply due to diffusion limitations, leading to compromised viability and impaired function. This challenge is especially relevant in large, engineered 3D constructs which exceed the diffusion limitation of 100 - 200 µm, resulting in necrotic cores. Granular hydrogels have emerged as a promising approach for tissue engineering due to their inherent porosity. Their interconnected pores can facilitate mass transport of oxygen and nutrients, and therefore support cellular function. In this work, we developed a facile method to prepare bioprintable granular hydrogel bioink containing microparticles with tunable diameters, that can form porous scaffolds with different pore sizes and porosities. The bioink remained shelf-stable for at least 60 days when stored at 4 °C, as determined by the melt temperature. The macroporous granular hydrogel scaffold has inherent void spaces between the hydrogel microparticles, in which spheroids can be embedded. Pancreatic spheroids incorporated into the scaffold showed improved viability compared to free spheroids, indicating the granular microenvironment supports spheroid survival. Furthermore, functional studies of embedded pancreatic spheroids with glucose-stimulated insulin secretion (GSIS) demonstrated preserved insulin secretion functionality. Our approach thus serves as a tunable platform for supporting the culture of functional spheroids and organoids, that has potential regenerative medicine applications in restoration of physiological functions when implanted.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149223027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural and functional remodeling of large-scale cardiac tubes.","authors":"Sára Szádocka, Jana Teske, Annika Franke, Mira Mertens, Nils Kriedemann, Merlin Witte, Lika Drakhlis, Arjang Ruhparwar, Ina Gruh, Robert Zweigerdt, Birgit Andrée","doi":"10.1088/1758-5090/aeadd2","DOIUrl":"10.1088/1758-5090/aeadd2","url":null,"abstract":"<p><p>The functionality of tissue-engineered cardiac constructs using human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) is challenged by the cells' immature state. To promote cell and tissue maturation, co-culture approaches, mechanical, electrical and topographical stimuli have been applied. Here, large tubular constructs (6 × 1 cm) were engineered from hiPSC-CMs and human foreskin fibroblasts (hFFs) (ratio 9:1) via a rotating mold technology in four independent experiments. A maturation regimen employing progressive uniaxial stretch imposed on cardiac tubes by a custom-designed, cost-efficient, fully three-dimensional printed stretching device ensured minimal manual intervention. Stepwise stretching (21% over 20 days) improved tissue contractility, alignment of hiPSC-CMs, sarcomere organization, and increased expression of cardiac structural genes, while maintaining tissue integrity and functionality in terms of spontaneous contractility and electrical responsiveness. The effect of modulating the stromal cell fraction on morphological and functional features of cardiac tubes was investigated in a pilot experiment. To more closely mimic native cardiac tissue and promote clinical translation, the originally applied hFFs were replaced with hiPSC-derived cardiac fibroblast-like cells. Compared with planar cardiac tissues dominating the field, our results provide translational insights into tubular constructs, better reflecting aspects of the native heart. Our progress in large-scale tissue engineering and functional outcomes paves the way toward establishing clinically relevant biological cardiac assist devices.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149079496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D printed graphene oxide/calcium alginate/strontium-doped hydroxyapatite composite scaffolds with enhanced osteogenic and angiogenic properties for critical-size bone defect repair.","authors":"Ma Chunquan, Wu Xudong, A Ze Lalai Di Li, Wang Yuxin, Zhang Hongxia, Xu Yang, Xu Biying, Ling Yunxiao, Ayijiamila Mohetaer, Liu Bin, Fuxiang Song","doi":"10.1088/1758-5090/aeadd1","DOIUrl":"10.1088/1758-5090/aeadd1","url":null,"abstract":"<p><p>The repair of critical-sized bone defects is hindered by insufficient vascularization, an unfavorable osteogenic microenvironment, and the limitations of conventional materials. Systematic studies on ternary composites of graphene oxide (GO), calcium alginate (CA), and strontium-doped hydroxyapatite (Sr-HA) remain limited, particularly regarding how different strontium substitution levels influence scaffold rheology, mechanics, ion release, osteogenic activity, and vascular-associated responses. Here, GO/CA/Sr-HA scaffolds (3D GCHs) were fabricated via direct ink writing with systematically controlled strontium substitution levels. All inks exhibited excellent shear-thinning behavior. The scaffolds showed a regular porous morphology and uniform strontium incorporation within the hydroxyapatite lattice. Notably, among the tested formulations, 3D GCH10 achieved a compressive stress of 3.42 ± 0.03 MPa at 70% strain and sustained Sr²⁺/Ca²⁺release for 28 d.<i>In vitro</i>, 3D GCH10 promoted bone marrow-derived mesenchymal stem cell proliferation, upregulated alkaline phosphatase, OPN, and RUNX2, enhanced mineralization, and showed no cytotoxicity. In a rat cranial defect model, BV/TV increased by 214.35% versus the blank group, with superior new bone formation, collagen deposition, and CD31/<i>α</i>-SMA-positive vascular density. These results suggest that appropriate strontium substitution combined with GO and CA contributes to improved scaffold properties and enhanced bone regeneration, highlighting the potential of 3D GCH10 for bone tissue engineering.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149079451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optogenetic-enabled biomanufacturing of long-projecting motor tracts for neural reconstruction.","authors":"Justin C Burrell, Vishal J Tien, Phuong T Vu, David R Clizbe, Michael Spadola, Yohannes Ghenbot, Ali K Ozturk, D Kacy Cullen","doi":"10.1088/1758-5090/aea7dc","DOIUrl":"10.1088/1758-5090/aea7dc","url":null,"abstract":"<p><p>Tissue-engineered spinal tracts (TE-STs), a subclass of micro-tissue-engineered neural networks, are designed to replace long-range axonal pathways but are limited by the slow intrinsic growth of many neuronal subtypes, particularly spinal motor neurons. Optogenetic stimulation provides cell-type-specific, precise control of activity that may accelerate neurite extension during biofabrication. Accordingly, this study evaluates whether integrating optogenetic stimulation into the biomanufacturing process enhances motor axonal outgrowth within tailored hydrogel micro-columns<i>in vitro.</i>Motor neuron aggregates from embryonic rat spinal cord were generated by forced aggregation and encased within agarose micro-columns (701<i>µ</i>m OD, ∼300<i>µ</i>m ID; 3-12 mm length) to create TE-STs. Neurons were transduced to express the red-shifted channelrhodopsin ChrimsonR. Stimulation parameters were first optimized in planar cultures, where a 1-hour red-light train at 10 Hz (625 nm) maximized neurite outgrowth without significant heating. TE-STs were then stimulated at 10 Hz for 1 h at 7 d<i>in vitro</i>(DIV) and then fixed at 21 DIV. Groups included transduced and non-transduced neurons, each with and without light stimulation. Axonal extension was quantified within the micro-column lumen, and two-way analysis of variance was used for analysis. TE-STs exhibited robust axonal growth with organized tract architecture, preserved neuronal health, and stable motor neuron phenotype. Transduced neurons with and without light stimulation extended axons faster than non-transduced controls, whereas no differences were observed between non-transduced neurons with or without light. Notably, transduced neurons exposed to a single round of optogenetic stimulation at 7 DIV reached ∼5.5 mm of growth by 21 DIV, representing a ∼2.5-fold increase over baseline controls. Brief, targeted optogenetic activation is sufficient to accelerate axonal growth of engineered spinal motor tracts during<i>in vitro</i>biomanufacturing. These findings establish optogenetic stimulation as a programmable biomanufacturing input that accelerates axonal assembly through activity-dependent mechanisms while remaining compatible with scalable three-dimensional tissue fabrication.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13659052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148975572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Airflow controlled droplet bioprinting enables high-throughput generation of iPSC-derived thymic organoids with<i>in vivo</i>T cell functionality.","authors":"Miranda Poklar, Erin Parlow, Ravikumar K, Nathan Ligenfelter, Mirabella Stump, Elyse Barnes, Lydia Kutzer, Wen Liu, Jiahui Dai, Lauren McCarthy, Carl Engman, Prashant N Kumta, Kimberly F Williams, Yong Fan, Bin Yang, Ipsita Banerjee","doi":"10.1088/1758-5090/aeae75","DOIUrl":"10.1088/1758-5090/aeae75","url":null,"abstract":"<p><p>The thymus is a critical organ for T cell generation. It is composed of distinct layers of thymic epithelial cells (TECs) that coordinate a complex process of antigen recognition and self-tolerance checks that result in mature populations of T cells. However, it undergoes progressive and permanent involution and loss of functionality which peaks in adolescence, rendering the tissue largely non-functional. Induced pluripotent stem cells (iPSCs) have emerged as promising candidates to generate organoid models of the thymus because of their autologous potential and indefinite self-renewal. Previously our group has shown the capability of generating thymic epithelial progenitor cells (TEPCs) that are able to successfully program T cells<i>in vitro</i>and<i>in vivo</i>through manual encapsulation of iPSCs in sodium-alginate capsules during differentiation. To translate this capability toward clinically relevant throughput, we present a novel method of air-jetting droplet bioprinting which is capable of producing 21 500 aggregates per milliliter of bioink. We conducted a systematic analysis of critical process parameters governing iPSC aggregation, retention, and differentiation and illustrated the interplay between airflow velocity, which governs capsule size, and crosslinker chemistry governing capsule network stability. We also determined parameters that minimize printing variability and prevent decapsulation of iPSC aggregates during differentiation. Finally, we modeled the growth rate and carrying capacity of different printing configurations, and validated manufactured organoids<i>in vivo</i>by generating functional murine T cell populations in athymic nude mice after decapsulation and construction with a decellularized thymus scaffold.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13660416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A 3D-printed spider silk-based functional mesh for ventral hernia repair via endogenous regeneration-promoting strategies.","authors":"Kai Deng, Changjun Liu, Xingjie Wang, Yanjun Sun, Xuetao Yuan, Enmeng Li, Gan Li, Shuting Yang, Lei Ma, Yiwei Ren, Zhengdong Jiang, Guangbing Wei, Er Meng, Xuqi Li","doi":"10.1088/1758-5090/aea9cf","DOIUrl":"10.1088/1758-5090/aea9cf","url":null,"abstract":"<p><p>Ventral hernia, a prevalent condition especially in the elderly, is primarily treated with mesh implantation. However, high postoperative recurrence persists due to mismatched degradation rates of biological meshes and tissue remodeling. We engineered an electrospun composite mesh (CPCM) integrating chitosan, polylactic acid, and chimeric mini-spidroin recombinant collagen to address this challenge. The CPCM was fabricated by combining 3D printing with bilateral electrospinning. Fourier-transform infrared spectroscopy, scanning electron microscopy, water contact-angle measurements, degradation assays, and uniaxial tensile testing were used to characterize its physicochemical and mechanical properties. Cytocompatibility, cell adhesion, antibacterial activity, and angiogenic effects were evaluated. A rat abdominal wall defect model was used to assess biocompatibility, vascularization, collagen remodeling, and repair efficacy through histological staining, immunofluorescence, Western blotting, and quantitative reverse transcription PCR. The CPCM exhibited favorable physicochemical and mechanical properties, gradual degradation, satisfactory cytocompatibility, enhanced cell adhesion, and significant antibacterial activity. CPCM implantation was associated with a repair-associated macrophage response and increased vascular density and vascular maturation during the early repair phase. At 12 weeks, the CPCM group exhibited greater collagen deposition, a higher collagen I/III ratio, more coherently organized collagen bundles, and improved mechanical performance of the repaired abdominal wall. molecular analyses further supported the involvement of TGF-<i>β</i>/Smad signaling in the fibroblast response to CPCM. CPCM demonstrated favorable physicochemical properties, antibacterial activity, and biocompatibility, and supported vascularization, repair-associated macrophage responses and collagen remodeling during abdominal wall repair. These findings suggest that CPCM may provide a promising multifunctional biodegradable mesh for abdominal wall reconstruction, while the contribution of TGF-<i>β</i>/Smad signaling to its regenerative effects warrants further investigation.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149001215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MLATE V3: An Open-Source Multi-Tissue AI Framework for Data-Driven Optimization of 3D-Printed and Bioprinted Scaffolds.","authors":"Saeed Rafieyan, Minoo Partovi Nasr, Elham Ansari, Alireza Kheradvar Kolour, Behnaz Banimohamad-Shotorbani, Ebrahim Vasheghani-Farahani","doi":"10.1088/1758-5090/aeb162","DOIUrl":"https://doi.org/10.1088/1758-5090/aeb162","url":null,"abstract":"<p><p>3D (bio)printing is increasingly used for scaffold fabrication in tissue engineering; however, identifying suitable biomaterial compositions, cell types, cell densities, and printing conditions remains largely empirical, time-consuming, and costly. Practical application of machine learning in this field is further limited by the scarcity of comprehensive datasets based on pre-fabrication variables, the lack of integrated frameworks for prioritizing candidate scaffold formulations, and the limited availability of accessible tools for non-technical users. To address these challenges, we developed MLATE V3 (Machine Learning Applications in Tissue Engineering V3), an open-source, data-driven, multi-tissue framework for scaffold prediction, prioritization, and experimental planning. An open-source dataset comprising 2,646 samples, 130 biomaterials, and 186 cell lines, including co-culture systems, across 18 tissue engineering contexts was compiled and used to benchmark machine learning, deep learning, tabular transformer-based, and tabular foundation models for predicting cell response and printability from pre-fabrication variables. The best-performing models achieved weighted F1-scores of 0.773 for cell response and 0.806 for printability. These predictive outputs were subsequently integrated through the Weighted Synergistic Scaffold Quality (WSSQ) metric, which combines cell response and printability using adjustable weights to provide a unified criterion for candidate ranking. WSSQ was then incorporated into a Bayesian optimization framework to search user-defined biomaterial compositions and printing-parameter ranges and prioritize candidate scaffold formulations for experimental evaluation. To make the complete workflow accessible to researchers, MLATE V3 was implemented as an open-source web platform that enables users to select predictive models, define design spaces, perform WSSQ-based optimization, and generate step-by-step fabrication protocols through an LLM-assisted procedure-generation module. Overall, MLATE V3 provides an extensible decision-support framework that connects literature-derived data, predictive modeling, multi-objective scaffold ranking, optimization, and experimental planning. The platform is intended to narrow the experimental search space and generate candidate formulations for subsequent experimental validation rather than to serve as experimentally validated optimizer.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149126642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perspectives on the future of biofabrication: insights from the winter school for biofabrication.","authors":"Oliver Kopinski-Grünwald, Corinna Barella, Anastasiia Budaeva, Jason A Burdick, Nathan Carpentier, Justin Cooper-White, Nikolas Galensowske, Antonia Georgopoulou, Jürgen Groll, Pavla Hajovska, Imanda Jayawardena, Mohammad Jouybar, Inga Jurgelane, Petra J Kluger, Marco Lai, Catherine Le Visage, Khoon S Lim, Jagoda Litowczenko, Marica Markovic, Lorenzo Moroni, Maryam Mousavi Movahed, Koichi Nakayama, Valentina Pavanati, Mina Petrovic, Felix Pointner, Heinz Redl, Christopher Riedmüller, Alice Salvadori, Simon Sayer, Stephan Schandl, Thomas Schmidt, Viktoria Schoen, Wojciech Święszkowski, Andrea Ulloa-Fernandez, Jasper Van Hoorick, Rathina Vel Balasubramanian, Michał Wszoła, Sing Yian Chew, Alessia Zanacchi, Marcy Zenobi-Wong, Aleksandr Ovsianikov","doi":"10.1088/1758-5090/ae6c5d","DOIUrl":"10.1088/1758-5090/ae6c5d","url":null,"abstract":"<p><p>Biofabrication is a multidisciplinary field that integrates diverse technologies to create three-dimensional structures composed of living cells, biomaterials, and other biological components. One of its main goals is the realization of functional tissue constructs with hierarchical architectures, as observed in native biological tissues, for applications in tissue engineering and regenerative medicine, as well as for<i>in vitro</i>modelling. Given its multidisciplinary nature, continued collaboration among experts from various scientific and technological disciplines remains essential to address the challenges ahead and to achieve the ambitious objectives of this rapidly advancing field. Reflecting on the progress made since the first bioprinting attempts, one can only envision the achievements that biofabrication may accomplish in the coming decades. Symposia such as the Alpine winter school for Biofabrication, bring together leading experts and early-stage researchers who collectively shape the future of the discipline through innovation and discussion. During the 2025 edition of the winter school, participants exchanged knowledge on current advances, challenges, and future perspectives in biofabrication. Guided by experts in this field, early-stage researchers discussed and summarized their views on five key topics: (i) the future of devices and technology in biofabrication, (ii) current trends and opportunities in material development, (iii) impact on society, ethics, and communication, (iv) applications and clinical translation, and (v) education and skillsets required for successful research. Their perspectives summarized here, address critical questions about future methodology, material development, and technological improvements that will continue to shape the evolution of biofabrication. Furthermore, aspects such as the impact on society, as well as the required skillset and education for future scientists working in the field are discussed.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":"18 4","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stochastic fiber templating for rapid generation of perfusable microchannels via immersed microfluidic spinning method.","authors":"Zarya Rajestari, Christopher T Clark, Quinton Smith, Lawrence Kulinsky","doi":"10.1088/1758-5090/aea7df","DOIUrl":"https://doi.org/10.1088/1758-5090/aea7df","url":null,"abstract":"<p><p>The fabrication of biomimetic tubular architectures within hydrogel-based tissue constructs remains a major barrier to advancing scalable tissue engineering and organoid systems. Here, we present a rapid and modular strategy for engineering hollow, perfusable channels within gelatin methacryloyl (GelMA) hydrogels using dissolvable calcium alginate fibers fabricated by an immersed microfluidic spinning method. This approach enables stochastic deposition of sacrificial fibers within a broad range of total construct sizes, creating tortuous and coiled architectures directly within soft hydrogel matrices. Upon UV crosslinking, the alginate fibers are dissolved using a cytocompatible calcium-chelator solution, yielding continuous, open cavities. Across a range of GelMA concentrations, the method produced stable channels compatible with cell-laden constructs. Viability assays with human dermal fibroblasts (HDF) confirmed >90% cell survival in regions adjacent to the channel within the diffusion limit, ranging from 700<i>µ</i>m in the softest 5% GelMA to 70<i>µ</i>m in the stiffest 15% GelMA, following fabrication and fiber dissolution. Furthermore, epithelial seeding of tortuous channels yielded perfusable structures with tight junctional function, demonstrating the method's potential to generate biologically relevant epithelial architectures. This accessible, equipment-light platform provides a versatile route for integrating microchannel networks into hydrogel systems for regenerative and<i>in vitro</i>modeling applications.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":"18 4","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}