Biofabrication最新文献

{"title":"3D bioprinting in tissue engineering: current state-of-the-art and challenges towards system standardization and clinical translation.","authors":"Tarun Agarwal, Valentina Onesto, Dishary Banerjee, Shengbo Guo, Alessandro Polini, Caleb D Vogt, Abhishek Viswanath, Timothy Esworthy, Haitao Cui, Aaron O'Donnell, Kiran Yellappa Vajanthri, Lorenzo Moroni, Ibrahim T Ozbolat, Angela Panoskaltsis-Mortari, Lijie Grace Zhang, Marco Costantini, Tapas Kumar Maiti","doi":"10.1088/1758-5090/ade47a","DOIUrl":"https://doi.org/10.1088/1758-5090/ade47a","url":null,"abstract":"<p><p>Over the past decade, 3D bioprinting has made significant progress, transforming into a key innovation in tissue engineering. Despite the early strides, critical challenges remain in 3D bioprinting that must be addressed to accelerate clinical translation. In particular, there is still a long way to go before functionally-mature, clinically-relevant tissue equivalents are developed. Current limitations range from the sub-optimal bioink properties and degree of biomimicry of bioprintable architectures, to the lack of stem/progenitor cells for massive cell expansion, and fundamental knowledge regarding in vitro culturing conditions. In addition to these problems, the absence of guidelines and well-regulated international standards is creating uncertainty among the biofabrication community stakeholders regarding the reliable and scalable production processes.&#xD;This review aims at exploring the latest developments in 3D bioprinting approaches, including various additive manufacturing techniques and their applications. A through discussion of common bioprinting techniques and recent progresses are compiled along with notable recent studies. Later we discuss the current challenges in clinical application of 3D bioprinting and the major bottlenecks in the commercialization of 3D bioprinted tissue equivalents, including the longevity of bioprinted organs, meeting biomechanical requirements, and the often underrated ethical and legal aspects. Amidst the progress of regulatory efforts for regenerative medicine, we also present an overview of the current regulatory concerns which should be taken into account to translate bioprinted tissues into clinical practice. At last, this review emphasizes future directions in 3D bioprinting that includes the transformative ideas like bioprinting in microgravity and the integration of artificial intelligence. The study concludes with the discussions on the need for collaborative efforts in resolving the technical and regulatory constraints to improve the quality, reliability, and reproducibility of bioprinted tissue equivalents to ultimately accomplish their successful clinical implementation.&#xD.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A hepatic zonation chip with an oxygen concentration gradient embracing the spatial distribution of metabolic function.","authors":"Min Kyeong Kim, Kyurim Paek, Kyungwon Park, Sungho Tak, Kyuhwan Na, Jung Hoon Choi, Sang-Mi Woo, HanByeol Kim, Yeo Min Yoon, Seok Chung, Jeong Ah Kim","doi":"10.1088/1758-5090/adde86","DOIUrl":"10.1088/1758-5090/adde86","url":null,"abstract":"<p><p>The development of hepatic<i>in vitro</i>models that replicate the physiological characteristics of liver tissue is critical for the accurate translation of drug test results. Current models often fail to mimic the spatial zonation by an oxygen concentration gradient in the hepatic acinus, limiting their ability to predict drug-induced hepatotoxicity. This study aimed to develop a hepatic zonation chip (H-chip) that replicates the oxygen gradient of the hepatic acinus, enhancing physiological relevance for drug testing applications. The H-chip was fabricated with a circular microfabricated chip chamber covered by oxygen-impermeable glass substrates, generating a radial oxygen concentration gradient through oxygen consumption by hepatic cells. This gradient mimics the portal-to-central oxygen distribution observed<i>in vivo</i>, enabling zone-specific hepatic functionality. We showed that the H-chip successfully reproduced the oxygen gradients found in the<i>in vivo</i>hepatic acinus along with corresponding cell cytocompatibility of hepatic cells. Notably, pericentral-specific hepatic functionality increased in the H-chip and decreased in the normoxia chip (N-chip). Spatial transcriptomic analysis revealed heterogeneous gene expression patterns aligned with local metabolic functions in each zone across the H-chip. Furthermore, toxicity evaluation of acetaminophen, a representative drug known for its spatial hepatotoxicity, revealed increased zonation-specific sensitivity in the H-chip, linked to elevated cytochrome P450 gene expression and toxic metabolite formation. These findings highlight the ability of the H-chip to replicate hepatic zonal characteristics, thus providing a robust platform for evaluating hepatotoxicity in drug testing. This platform promises to advance safer and more effective drug development by enabling more physiologically relevant assessments.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress in the application of organoids for exploring the relationship between macrophages and various lung diseases.","authors":"Jiawang Wu, Ting Liu, Xinting Zhang, Chongchang Qu, Juan Wu, Shuanglan Xu, Jiao Yang, Xiqian Xing","doi":"10.1088/1758-5090/adde15","DOIUrl":"10.1088/1758-5090/adde15","url":null,"abstract":"<p><p>Organoids cultured<i>ex vivo</i>mimic<i>in vivo</i>tissue and organ characteristics. They have become a focus in research for their potential in modeling macrophage function in respiratory diseases, offering insights into disease mechanisms and therapeutic strategies. A number of studies have confirmed organoids' utility in dissecting microbial interactions, disease modeling, genetic manipulation, and high-throughput drug screening for efficacy and safety. This review summarizes the research progress on organoids in exploring macrophage involvement in pulmonary diseases.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioengineering fascicle-like skeletal muscle bioactuators via pluronic-assisted co-axial 3D bioprinting (PACA-3D).","authors":"Judith Fuentes, Rafael Mestre, Maria Guix, David Esporrín-Ubieto, Ibtissam Ghailan Tribak, Noelia Ruiz-González, Tania Patiño, Samuel Sánchez","doi":"10.1088/1758-5090/addc9b","DOIUrl":"10.1088/1758-5090/addc9b","url":null,"abstract":"<p><p>Advances in 3D bioprinting have opened new possibilities for developing bioengineered muscle models that can mimic the architecture and function of native tissues. However, current bioengineering approaches do not fully recreate the complex fascicle-like hierarchical organization of the skeletal muscle tissue, impacting on the muscle maturation due to the lack of oxygen and nutrient supply in the scaffold inner regions. A key challenge is the production of precise and width-controlled independent filaments that do not fuse during the printing process when subsequently extruded, ensuring the formation of fascicle-like structures. This study addresses the limitation of filament fusion by utilizing a pluronic-assisted co-axial 3D bioprinting system (PACA-3D) creates a physical confinement of the bioink during the extrusion process, effectively obtaining thin and independent printed filaments with controlled shapes. The use of PACA-3D enabled the fabrication of skeletal muscle-based bioactuators with improved cell differentiation and significantly increased force output, obtaining 3 times stronger bioengineered muscle when compared to bioactuators fabricated using conventional 3D extrusion bioprinting techniques, where a single syringe containing the bioink is used. The versatility of our technology has been demonstrated using different biomaterials, demonstrating its potential to develop more complex biohybrid tissue-based architectures with improved functionality, as well as aiming for better scalability and printing flexibility.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144131868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: Dual-core coaxial bioprinting of double-channel constructs with a potential for perfusion and interaction of cells (2022<i>Biofabrication</i>14 035012).","authors":"Yanrong Yu, Renjian Xie, Yueteng He, Furong Zhao, Quan Zhang, Wei Wang, Yong Zhang, Jiawei Hu, Dan Luo, Weijie Peng","doi":"10.1088/1758-5090/addd4b","DOIUrl":"https://doi.org/10.1088/1758-5090/addd4b","url":null,"abstract":"","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":"17 3","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D printed bioactive coated scaffolds boost osteogenesis and angiogenesis via the regulation of scaffold microstructure.","authors":"Yulin Jiang, Chen Zhou, Xi Yang, Dongxu Ke","doi":"10.1088/1758-5090/addc9c","DOIUrl":"10.1088/1758-5090/addc9c","url":null,"abstract":"<p><p>Microstructure plays a crucial role in bone regeneration, conventional bone tissue engineering scaffold fabrication techniques often lack the precision required to control microstructural features that can optimize bone healing. 3D printing, as a powerful tool for biofabrication, allows for the design and optimization of scaffold microstructures to enhance bone healing. In this study, bioactive coated scaffolds composed of polycaprolactone and tricalcium phosphate were fabricated using a micro-extrusion 3D printer with varying compositions and microstructures, resulting in different physical and mechanical properties. Among these properties, porosity and permeability played a vital role in osteogenic and angiogenic differentiation.<i>In vitro</i>studies revealed that the permeability effect was dominant in osteogenic differentiation, while the porosity effect mainly induced the angiogenic differentiation, with potential mechanisms involving crosstalk between Wnt and PI3K signaling pathways. Moreover, significantly improved osteogenesis and angiogenesis were observed in U600 scaffolds compared to sham and U300 scaffolds, supporting the<i>in vitro</i>findings. This study provides valuable insights for the microstructure optimization of 3D printed tissue engineering scaffolds, which could facilitate the translation of 3D printing technology from the benchside to clinical applications.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144131865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in microfluidic biofabrication technology for bone metastasis modeling.","authors":"Mehdi Khanmohammadi, Nima Ahmadkhani, Marina Volpi, Khadijeh Khederlou, Alankrita Uppal, Mahdis Hosseini, Yu Shrike Zhang, Wojciech Swieszkowski","doi":"10.1088/1758-5090/add95f","DOIUrl":"10.1088/1758-5090/add95f","url":null,"abstract":"<p><p>Studying bone metastasis in<i>in vitro</i>models is essential for understanding the mechanisms driving this process, developing effective therapeutic strategies, and evaluating potential treatments for metastatic cancer patients. To this end, traditional two-dimensional (2D) cell culture models fail to replicate the native three-dimensional (3D) tissue microenvironment, resulting in significant disparities in biologically relevant behaviors and drug responses. The shift from 2D to 3D cell culture techniques represents an important step toward creating more biomimetic bone metastasis models. These systems more effectively emulate and replicate the complex interactions between cancer cells and bone tissue, including essential cell-cell and cell-extracellular matrix interactions, as well as<i>in vivo</i>biomechanical cues. The development and application of microfluidic-based 3D cancer models, incorporating diverse shapes, architectures, and modular structures such as organ-on-chip platforms, enable comprehensive screening and exploration of cellular interplay, the dissection of signaling pathways, and the resolution of limitations associated with traditional models. This review highlights recent advancements in microfluidic-based 3D bone metastasis models and examines innovative applications of this technology. These include hydrogel-based spherical and filaments biofabrication approaches, 2D and 3D tumor on-a- chips, and drug screening techniques such as concentration gradient generator-based, microdroplet-based, and microarray-based chips, as well as tumor tissue chips. Additionally, we discuss the benefits and limitations of these approaches in treating bone metastases and propose future directions for advancing microfluidic platforms in drug discovery and this research field.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic bioprinting as a tool to produce hiPSCs-derived renal organoids.","authors":"Chiara Formica, Gabriele Addario, Sveva Fagiolino, Lorenzo Moroni, Carlos Mota","doi":"10.1088/1758-5090/addb7e","DOIUrl":"10.1088/1758-5090/addb7e","url":null,"abstract":"<p><p>Chronic kidney disease affects 10% of the global population and often progresses to end-stage renal disease, where dialysis or renal transplant are the only therapies, though neither is a permanent solution. Regenerative medicine, particularly the use of organoids, offers a potential solution. Organoids are valuable for studying organ development, diseases, and regeneration, and are suitable for drug screening. However, their limited ability to replicate adult organs' maturation, complexity, and functions restricts their application. Additionally, manual production of organoids causes variability, affecting scalability and reproducibility. Automation techniques like bioprinting could enhance organoid maturation and complexity by depositing cells and biomaterials in a controlled manner. In this study, we established differentiation protocols to obtain human induced pluripotent stem cell-derived metanephric mesenchyme, ureteric bud progenitors, and the combination of these was used to form organoids. A microfluidic bioprinter capable of producing core-shell filaments was used to bioprint single cell progenitors in combination with gelatin in the core wrapped with an alginate shell. These filament constructs were cultured with an optimized mixture of growth factors for two weeks. Within one week, renal vesicles were visible, and after two weeks post-bioprinting the kidney organoids were functional and respond to the nephrotoxic drug doxorubicin. In conclusion, a bioprinted method was developed to generate in an automated way functional renal organoids from progenitors, offering a foundation for future kidney disease treatment.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofabrication in suspension media-a decade of advances.","authors":"Megan E Cooke, Morgan B Riffe, Manuela E Gomes, Rui M A Domingues, Jason A Burdick","doi":"10.1088/1758-5090/addc42","DOIUrl":"10.1088/1758-5090/addc42","url":null,"abstract":"<p><p>Suspension bath bioprinting, defined as extrusion bioprinting into a suspension bath consisting of a yield-stress material with fast recovery, emerged over a decade ago. Since this time, many suspension baths have been developed from molecular assemblies to granular media and across a range of synthetic and natural polymers. These suspension baths have been applied to the printing of a wide variety of inks for applications in tissue engineering, from<i>in vitro</i>tissue models to implantable constructs. In a scoping search of published literature over the past decade, 254 articles were identified that met various definitions related to suspension baths for biofabrication in order to gain a perspective on the various materials used and their applications; however, the literature is much more broad than this due to the disperse terminology that has been applied to the approach. This article gives a perspective on the progress that has been made in suspension bath printing, including applications of the technology and challenges that exist across the field, as well as provides a look to the future of where such printing methods will make an impact.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12131275/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofabrication of microstructured bacterial ecosystems using chaotic bioprinting: advancing<i>in vitro</i>research for microbial engineering.","authors":"Ariel Cantoral-Sánchez, Oscar Emmanuel Solís-Pérez, Francisco Javier Flores-Loera, Claudia Maribel Luna-Aguirre, Luis Fernando Carmona-Ramirez, Ilsa Pamela De Los Santos-Hernández, Nora Greys Zamora-Benavides, Mara Neher, Grissel Trujillo de Santiago, Mario Moisés Alvarez","doi":"10.1088/1758-5090/add568","DOIUrl":"10.1088/1758-5090/add568","url":null,"abstract":"<p><p>Mixed microbial communities are essential for various ecosystems, with bacteria often exhibiting unique behaviors in structured environments. However, replicating these interactions<i>in vitro</i>remains challenging, as traditional microbiology techniques based on well-mixed cultures fail to capture the spatial organization of natural communities. Chaotic 3D printing offers a versatile, high-throughput method for fabricating hydrogel constructs with multilayered microstructure in which different bacterial strains can coexist, closely mimicking the partial segregation seen in natural microbial ecosystems. Using a Kenics static mixer printing nozzle, we bioprinted a bacterial consortium consisting of<i>Lactobacillus rhamnosus, Bifidobacterium bifidum</i>, and<i>Escherichia coli</i>as a simplified model for human gut microbiota. Chaotic bioprinting enabled the creation of microstructured cocultures with distinct niches, allowing all bacterial strains to coexist (without being scrambled) and reach a population equilibrium. We characterized the cocultures through fluorescence microscopy, colony counting, and quantitative polymerase chain reactions. Our results demonstrate that the microarchitecture of the printed fibers significantly influences bacterial growth dynamics. Stratified arrangements enhanced coculture viability and balance over 72 h compared to well-mixed and suspension conditions. Chaotic printing also allows the rational arrangement of strict anaerobic bacteria, such as<i>B. bifidum</i>, by positioning them in construct layers that are more susceptible to hypoxia. Chaotic bioprinting presents a powerful tool for engineering microbial ecosystems with precise spatial control in the range of tens of micrometers. This approach promises to advance our understanding of microbial interactions and has potential biomedical applications in antibiotic testing, microbiota research, bioremediation, and synthetic biology.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}