Biofabrication最新文献

{"title":"Multifunctional 4D printed shape memory composite scaffolds with photothermal and magnetothermal effects for multimodal tumor therapy and bone repair.","authors":"Jingguang Wang, Jielong Zhou, Zhenze Xie, Yunhui Zhang, Muye He, Tianyu Wei, Shibin Wu, Chang Du","doi":"10.1088/1758-5090/adc29e","DOIUrl":"10.1088/1758-5090/adc29e","url":null,"abstract":"<p><p>Tumor recurrence and bone defects are two key challenges in the surgical treatment of osteosarcoma (OS). Therefore, it is highly necessary to develop a multifunctional scaffold that can simultaneously eradicate tumor cells and promote bone regeneration. Herein, a hierarchically porous shape memory scaffold consisting of hydroxyapatite, silica, poly(D,L-lactide-co-trimethylene carbonate) and Fe₃O₄(HSP-Fe₃O₄) is constructed by Pickering emulsion and 4D printing technique. The HSP-Fe₃O₄scaffold demonstrates the advantages of multimodal anti-tumor therapy, including chemotherapy through the Fenton reaction, effective photothermal conversion for photothermal therapy under near-infrared laser irradiation, and magnetothermal therapy provided by an alternating magnetic field. Furthermore, photothermal hyperthermia also serve as triggers for the shape memory effect of the HSP-Fe₃O₄scaffold, enabling the scaffold to precise adaptation of complex bone defects after minimally invasive surgical implantation. Additionally, the HSP-Fe₃O₄scaffold with interconnected multiscale pore exhibits good biocompatibility and excellent bone repair capabilities. This study proved that the HSP-Fe₃O₄scaffold provides positive insights for preventing tumor recurrence and facilitating bone regeneration after OS surgery.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced gelatin methacryloyl nanohydroxyapatite hydrogel for high-fidelity 3D printing of bone tissue engineering scaffolds.","authors":"Toufik Naolou, Nadine Schadzek, Jan Mathis Hornbostel, Iliyana Pepelanova, Miriam Frommer, Franziska Lötz, Leopold Sauheitl, Stefan Dultz, Vincent J M N L Felde, Ola Myklebost, Cornelia Lee-Thedieck","doi":"10.1088/1758-5090/adbb90","DOIUrl":"10.1088/1758-5090/adbb90","url":null,"abstract":"<p><p>Patients suffering from large bone defects are in urgent need of suitable bone replacements. Besides biocompatibility, such replacements need to mimic the 3D architecture of bone and match chemical, mechanical and biological properties, ideally promoting ossification. As natural bone mainly contains collagen type I and carbonate hydroxyapatite, a 3D-printable biomaterial consisting of methacrylated gelatin (GelMA) and nanohydroxyapatite (nHAp) would be beneficial to mimic the composition and shape of natural bone. So far, such nanocomposite hydrogels (NCH) suffered from unsatisfactory rheological properties making them unsuitable for extrusion-based 3D printing with high structural fidelity. In this study, we introduce a novel GelMA/nHAp NCH composition, incorporating the rheological modifier carbomer to improve rheological properties and addressing the challenge of calcium cations released from nHAp that hinder GelMA gelation. Leveraging its shear-thinning and self-healing properties, the NCH ink retains its shape and forms cohesive structures after deposition, which can be permanently stabilized by subsequent UV crosslinking. Consequently, the NCH enables the printing of 3D structures with high shape fidelity in all dimensions, including the<i>z</i>-direction, allowing the fabrication of highly macroporous constructs. Both the uncured and the UV crosslinked NCH behave like a viscoelastic solid, with<i>G</i>'><i>G</i>″ at deformations up to 100-200 %. After UV crosslinking, the NCH can, depending on the GelMA concentration, reach storage moduli of approximately 10 to over 100 kPa and a mean Young's Modulus of about 70 kPa. The printed scaffolds permit not only cell survival but also osteogenic differentiation, highlighting their potential for bone tissue engineering.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A self-assembling peptide-based hydrogel containing NF-<i>κ</i>B inhibitors and NGF for peripheral nerve injury repair.","authors":"HaiTao Su, QiuPing Ye, Di Wang, AnQi Liu, YaGuang Wang, Ye Zhang, LiJun Weng","doi":"10.1088/1758-5090/adc340","DOIUrl":"https://doi.org/10.1088/1758-5090/adc340","url":null,"abstract":"<p><p>An inflammatory response may be initiated after peripheral nerve injury (PNI), potentially hindering the repair and regeneration of damaged nerves. Administering anti-inflammatory agents to modulate macrophage phenotypes may reduce post-injury inflammation and show potential for treating PNI. Regrettably, the limited half-lives of these compounds within the human body constrain their efficacy as anti-inflammatory agents. In this study, we co-assembled picroside II (PII) and nerve growth factor (NGF) with the hydrogelator compound Nap-Phe-Phe-Tyr-OH (NapFFY) to form a supramolecular hydrogel, PII/NGF/NapFFY@Gel, which could be accurately delivered to the nerve injury site via<i>in situ</i>injection to improve its bioavailability. Our results demonstrated that the PII/NGF/NapFFY@Gel exhibits favorable drug slow-release performance in both<i>in vivo</i>and<i>in vitro</i>experiments. Furthermore, cell and animal studies revealed that the PII/NGF/NapFFY@Gel effectively enhanced nerve recovery and regeneration by modulating the inflammatory microenvironment. This mechanism involves inhibiting the NF-<i>κ</i>B inflammatory signaling pathway, suppressing macrophage polarization to the M1 phenotype, and upregulating the expression of proteins associated with nerve regeneration. Taken together, the results of this study suggest that improving the inflammatory microenvironment and promoting nerve repair through the<i>in situ</i>injection of PII/NGF/NapFFY@Gel with sustained drug release may be a novel treatment for PNI.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":"17 2","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient one-step immobilization of DNA probes on 1DZnO nanoplatforms targeting a low-mutation region of SARS-CoV-2.","authors":"Shirlley Martínez Tolibia, Rafael A Salinas, Cesar Millán-Pacheco, Edgar O Castrejón González, Erik A Vázquez-Montelongo, Josué E Romero, Guillermo Santana, Ateet Dutt","doi":"10.1088/1758-5090/adc159","DOIUrl":"10.1088/1758-5090/adc159","url":null,"abstract":"<p><p>Fabricating cost-effective biosensors with rapid response times is highly desirable during pandemic scenarios, where accuracy, swift detection, and portability are crucial for making prompt decisions. The design and conceptualization of these devices at early stages are critical for enhancing their output responses. In this work, we implemented a one-step immobilization strategy for DNA probes targeting a low-mutation region from the envelope protein of SARS-CoV-2 onto one-dimensional ZnO nanostructures (1DZnO) to achieve high detection efficiency. First, DNA probes were designed to select a highly conserved region (L28-A36) among SARS-CoV-2 subvariants using bioinformatic analysis. Then, dynamic simulations were performed to estimate the binding affinity of DNA to 1DZnO, where phosphate molecules were identified as the functional groups with the highest affinity to the ZnO surface, followed by the sugar rings and the base pairs. In addition, linear interaction energies and their average contributions were calculated for the ssDNA/ZnO interfaces. Computational simulations were correlated to experimental techniques, where suitable DNA immobilization and target detection were confirmed by FTIR, photoluminescence (PL), transmission electron microscopy, and elemental mapping, corroborating the adsorption of DNA across the entire 1DZnO surface. Intense peaks related to C-C, C=C, C=N, P-O, and N-H were identified as the most important by FTIR characterizations, whereas PL showed a distinctive shift in deep level emission band between 520-530 nm, with a partial quenching of the near band emission signal, obtaining as well variations in the calculated bandgap. In summary, it is suggested that structural oxygen vacancies of 1DZnO nanoplatforms provide a significant proportion of active available sites for an easy and strong interaction with the phosphate backbone of DNA, enhancing physical adsorption. Furthermore, molecular validation by PCR confirmed the long-term stability of immobilized DNA probes, probing their suitability for further biosensing devices.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxytocin-loaded hydrogel promotes cartilage regeneration and regulates microenvironment.","authors":"Tianming Wang, Xiao Zhao, Jiayi Li, Chongchong Yin, Bo Jiang, Jiaming Xie, Binghao Wang, Yufeng Wang, Zhicheng Cao, Qingqiang Yao, Shengnai Zheng, Jisheng Sui, Kun Zhu","doi":"10.1088/1758-5090/adc158","DOIUrl":"10.1088/1758-5090/adc158","url":null,"abstract":"<p><p>Osteoarthritis is a common orthopedic condition, and traditional treatment methods often fail to regenerate cartilage effectively. Oxytocin (OXT) is a neuropeptide that plays a crucial role in the skeletal system. Hyaluronic acid (HAMA) hydrogel has emerged as a key carrier for cartilage repair due to its excellent biocompatibility and biodegradability. Combining OXT with HAMA hydrogel and implanting it at the site of cartilage defects can effectively promote cartilage regeneration. Cartilage damage often results in an altered microenvironment, characterized by macrophage polarization and high levels of reactive oxygen species (ROS). Oxidative stress can stimulate macrophages to produce more pro-inflammatory factors. OXT can inhibit the secretion of pro-inflammatory cytokines such as TNF-<i>α</i>, IL-6, and IL-1<i>β</i>by interacting with the STAT3/NF-<i>κ</i>B signaling pathway, as well as the PI3K/Akt and mitogen-activated protein kinase pathways, thereby inducing the polarization of macrophages from the M1 phenotype to the M2 phenotype and alleviating the inflammatory response. OXT can also enhance the expression of NRF and HO-1, which helps eliminate ROS and suppress the expression of pro-inflammatory factors. Regulating the microenvironment of cartilage damage is beneficial for cartilage protection and repair. OXT activates the CFOS/AP-1 and STAT1/JAK2 pathways, which together act on MMP2 and MMP9 to alleviate cartilage degeneration. The STAT1/JAK2 pathway can further increase the expression of Col2, thereby protecting chondrocytes. Additionally, OXT can directly boost the protein levels of SOX9 and COMP, promoting chondrocyte proliferation and cartilage protection, ultimately achieving the therapeutic goal for arthritis. This study explores the potential of HAMA hydrogel as a delivery system for OXT and analyzes their impact on cartilage regeneration and anti-inflammatory properties. This research provides a novel strategy for the treatment of cartilage injuries.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomedical implication of microfluidics in disease diagnosis and therapeutics: from fabrication to prognosis.","authors":"Shivani Yadav, Manish Dwivedi, Sukriti Singh, Pooja Jangir","doi":"10.1088/1758-5090/adc0c2","DOIUrl":"10.1088/1758-5090/adc0c2","url":null,"abstract":"<p><p>Microfluidics has given us an approach to regulate the fluids' behaviour and influence at the microscale level, including the microchannels as an integral element. Microchannels encompass the high surface area-to-volume ratio, causing the rapid diffusion and mixing of substances within the tiny canals and facilitating predictable and stable fluid dynamics. This precise regulatory mechanism of fluid behaviour by microchannels is significant for several biological and chemical processes. In the present scenario, microfluidics plays a significant role in pharmaceutical industries for efficient drug synthesis, DNA analysis, protein crystallization and cell culture. They have also been exploited in fabricating site-directed drug delivery systems such as microchannels. This review has illustrated the different strategies for fabricating microfluidic devices (e.g. microchannels) and their potential implications in biomedical sciences. It also includes a discussion about the challenges associated with standardisation, cost-effective production, biocompatibility and safety concerning microchannel fabrication and its biological application, as well as possible approaches to overcome these issues. These microfluidic devices have the potential for diagnosis, drug delivery, disease monitoring and other applications in human health and diseases and require more attention from researchers to fabricate them precisely and efficiently.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: Gelatin methacryloyl and Laponite bioink for 3D bioprinted organotypic tumor modeling (2023<i>Biofabrication</i> 15 045005).","authors":"Natan Roberto de Barros, Alejandro Gomez, Menekse Ermis, Natashya Falcone, Reihaneh Haghniaz, Patric Young, Yaqi Gao, Albert-Fred Aquino, Siyuan Li, Siyi Niu, RunRun Chen, Shuyi Huang, Yangzhi Zhu, Payam Eliahoo, Arthur Sun, Danial Khorsandi, Jinjoo Kim, Jonathan Kelber, Ali Khademhosseini, Han-Jun Kim, Bingbing Li","doi":"10.1088/1758-5090/adbfc3","DOIUrl":"https://doi.org/10.1088/1758-5090/adbfc3","url":null,"abstract":"","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":"17 2","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Graphene oxide and<i>in-situ</i>carbon reinforced hydroxyapatite scaffolds via ultraviolet-curing 3D printing technology with high osteoinductivity for bone regeneration.","authors":"Hongyu Zhao, Xiao Niu, Shitong Wei, Wei Lin, Hao Luo, Bin Zou, Qinghua Chen, Hongyu Xing, Qingguo Lai","doi":"10.1088/1758-5090/adbcdd","DOIUrl":"10.1088/1758-5090/adbcdd","url":null,"abstract":"<p><p>Ultraviolet photopolymerization additive manufacturing has been used to fabricate calcium phosphate (Ca-P) ceramic scaffolds for repairing bone defects, but it is still a challenge for 3D printed Ca-P scaffolds to simultaneously enhance the mechanical strength and osteoinductivity. Here, we successfully developed a high-performance hydroxyapatite (HA) scaffold containing<i>in-situ</i>carbon and graphene oxide (GO) by precisely regulating the degreasing and sintering atmosphere. The results indicated that the mechanical properties of HA scaffolds could be significantly improved by regulating the amount of<i>in-situ</i>carbon. The HA scaffold containing 0.27 wt.% carbon achieved the maximum compressive strength of 12.5 MPa with a porosity of approximately 70%. The RNA transcriptome sequencing analysis revealed that<i>in-situ</i>carbon could promote osteogenic differentiation by improving oxygen transport and promoting the expression of multiple angiogenic factors. More importantly, in the absence of osteoinductive agents, the<i>in-situ</i>carbon and GO synergistically promoted more effective bone mineralization, demonstrating enhanced osteoinductivity<i>in vitro.</i>In a rodent model, the bioceramic scaffolds also exhibited improved osteogenesis in critical bone defects. Therefore,<i>in-situ</i>carbon and GO could simultaneously enhance the mechanical strength and osteoinductivity of HA scaffolds, effectively achieving substantial endogenous bone regeneration. This strategy will provide a simple and energy-efficient approach for engineering osteoinductive ceramic scaffolds for repairing bone defects.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofabrication and simulation techniques for gut-on-a-chip.","authors":"Ke Wang, Yushen Wang, Junlei Han, Zhixiang Liang, Wenhong Zhang, Xinyu Li, Jun Chen, Li Wang","doi":"10.1088/1758-5090/adb7c1","DOIUrl":"10.1088/1758-5090/adb7c1","url":null,"abstract":"<p><p>Biomimetic gut models show promise for enhancing our understanding of intestinal disorder pathogenesis and accelerating therapeutic strategy development. Current<i>in vitro</i>models predominantly comprise traditional static cell culture and animal models. Static cell culture lacks the precise control of the complex microenvironment governing human intestinal function. Animal models provide greater microenvironment complexity but fail to accurately replicate human physiological conditions due to interspecies differences. As the available models do not accurately reflect the microphysiological environment and functions of the human intestine, their applications are limited. An optimal approach to intestinal modeling is yet to be developed, but the field will probably benefit from advances in biofabrication techniques. This review highlights biofabrication strategies for constructing biomimetic intestinal models and research approaches for simulating key intestinal physiological features. We also discuss potential biomedical applications of these models and provide an outlook on multi-scale intestinal modeling.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ATPS-enabled single-step printing of chemically and mechanically on-demand tunable perfusable channels in ejectable constructs.","authors":"Malin Becker, Francisca Gomes, Isa Porsul, Jeroen Leijten","doi":"10.1088/1758-5090/adbcdc","DOIUrl":"10.1088/1758-5090/adbcdc","url":null,"abstract":"<p><p>3D bioprinting approaches offer highly versatile solutions to replicate living tissue and organ structures. While current bioprinting approaches can generate desired shapes and spatially determined patterns, the material selection for embedded bioprinting has remained limited, as it has relied on the use of viscous, shear-thinning, or liquid-like solid materials to create shape controlled constructs, which could then be modified downstream via multi-step processes. We here explore aqueous two-phase system stabilized 3D bioprinting of low viscous materials in combination with supramolecular complexation to fabricate intricate, perfusable engineered constructs that are both mechanically and chemically tunable in a single-step manner. To this end, we introduce Dex-TAB as a highly versatile backbone, that allows for mechanical and chemical tuning during as well as after printing. To showcase the printability as well as spatial chemical modification and mechanical tunability of this material, ejectability, and local/gradual or bulk functionalized interconnected tube shaped constructs were generated. Subsequently, we demonstrated that these functionalized channels could be printed directly into a syringe containing crosslinkable polymer solution, which upon ejection forms pre-patterned perfusable constructs. In short, we report that ATPS enabled low viscous 3D bioprinting can produce highly functional and even potentially minimally invasive injectable yet functionalized and perfusable constructs, which offers opportunities to advance various biofabrication applications.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}