Biofabrication最新文献_第3页

Additive manufacturing of silicon nitride fiber-reinforced polyetheretherketone composites with enhanced mechanical strength and multifunctional bioactivity for load-bearing bone defect repair. 增材制造具有增强机械强度和多功能生物活性的氮化硅纤维增强聚醚醚酮复合材料用于承重骨缺损修复。

IF 8.2 2区医学

Biofabrication Pub Date : 2025-05-29 DOI: 10.1088/1758-5090/add9d3

Shengxin Zeng, Haozheng Li, Panpan Hu, Zihe Li, Zhengguang Wang, Jiedong Wang, Jiasheng Chen, Shouzhan Wang, Gong Wang, Wei Zhao, Feng Wei

{"title":"Additive manufacturing of silicon nitride fiber-reinforced polyetheretherketone composites with enhanced mechanical strength and multifunctional bioactivity for load-bearing bone defect repair.","authors":"Shengxin Zeng, Haozheng Li, Panpan Hu, Zihe Li, Zhengguang Wang, Jiedong Wang, Jiasheng Chen, Shouzhan Wang, Gong Wang, Wei Zhao, Feng Wei","doi":"10.1088/1758-5090/add9d3","DOIUrl":"10.1088/1758-5090/add9d3","url":null,"abstract":"Polyether ether ketone (PEEK) is increasingly applied in bone defect repair due to its excellent biocompatibility and absence of artifact formation. However, the bio-inertness and inadequate mechanical properties of untreated PEEK remain significant challenges for PEEK-based implants. Hence, this study prepares a series of silicon nitride (Si3N4) fiber-reinforced PEEK composite porous scaffolds using twin-screw melt mixing-extrusion and material extrusion 3D printing. Comprehensive evaluations assess the mechanical properties, biocompatibility, osteogenic differentiation, angiogenesis activities, and antibacterial performances of various composites. Characterization results show that Si3N4fiber-reinforced PEEK composites exhibit excellent printability, with well-oriented Si3N4fibers uniformly distributed throughout the matrix. Furthermore, compared to non-reinforced PEEK, the addition of 8% Si3N4fibers enhanced Young's modulus by 52.2% (6.36 GPa). Additionally, bothin vitroandin vivoresults indicate that all composite scaffolds exhibit excellent biocompatibility. Notably, the 8% Si3N4fiber-reinforced PEEK composite demonstrated optimal multifunctional performance in osteogenic induction, angiogenic capacity, and antibacterial efficacy, significantly outperforming other experimental groups. In conclusion, this study offers a solution for enhancing the mechanical, anti-infective, and osseointegrative properties of PEEK, demonstrating its great potential for expanding the application of non-metallic orthopedic implants in bone defect repair.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High resolution melt electro-written scaffolds promote alignment of human skeletal muscle cells. 高分辨率熔融电写支架促进人体骨骼肌细胞排列。

IF 8.2 2区医学

Biofabrication Pub Date : 2025-05-28 DOI: 10.1088/1758-5090/add960

Finn Snow, Cathal O'Connell, Aaron Elbourne, Magdalena Kita, Peiqi Yang, Richard J Williams, Simon E Moulton, Elena Pirogova, Robert Michail Ivan Kapsa, Anita Quigley

{"title":"High resolution melt electro-written scaffolds promote alignment of human skeletal muscle cells.","authors":"Finn Snow, Cathal O'Connell, Aaron Elbourne, Magdalena Kita, Peiqi Yang, Richard J Williams, Simon E Moulton, Elena Pirogova, Robert Michail Ivan Kapsa, Anita Quigley","doi":"10.1088/1758-5090/add960","DOIUrl":"10.1088/1758-5090/add960","url":null,"abstract":"Advanced tissue engineering (TE) strategies are vital to address challenging musculoskeletal conditions, such as volumetric muscle loss. These disorders impose a considerable economic burden and affect individuals' quality of life, highlighting the need for innovative treatments, such as TE, to address these challenges. Here, we examine how scaffold fibre orientation influences mechanical properties and cellular behaviour by utilising melt electrowriting (MEW) as a high-resolution 3D printing technique that combines aspects of electrospinning and melt based polymer deposition. In this work, we investigated the effects of fibre orientation in MEW scaffolds, and its effect on the scaffold mechanical properties as well as cell orientation and alignment. MEW scaffolds were mechanically characterised through uniaxial strain testing to determine critical parameters, including strain at failure, ultimate tensile strength, Young's modulus (E), fatigue rate, recovery time, and yield strain. These mechanical properties were analysed to define an optimal strain regime for transitioning from static to dynamic culture conditions under muscle-like cyclic loading, relevant to muscle's viscoelastic behaviour. In parallel, static cultures of primary human skeletal muscle myoblasts and normal human dermal fibroblasts (NHDFs) were grown on MEW scaffolds, with varying architectures, to study the effects of fibre aspect ratio on cell alignment. Cell alignment was visualised using DAPI/phalloidin staining and quantified with the ImageJ directionality plugin, enabling a systematic comparison of scaffold designs. This approach evaluates the potential of supportive scaffold architectures to promote aligned cell growth, offering insights into designing effective scaffolds for tissue regeneration.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Controlled microvasculature for organ-on-a-chip applications produced by high-definition laser patterning. 控制微血管器官芯片上的应用产生高清晰度激光图案。

IF 8.2 2区医学

Biofabrication Pub Date : 2025-05-27 DOI: 10.1088/1758-5090/add37e

Alice Salvadori, Masafumi Watanabe, Marica Markovic, Ryo Sudo, Aleksandr Ovsianikov

{"title":"Controlled microvasculature for organ-on-a-chip applications produced by high-definition laser patterning.","authors":"Alice Salvadori, Masafumi Watanabe, Marica Markovic, Ryo Sudo, Aleksandr Ovsianikov","doi":"10.1088/1758-5090/add37e","DOIUrl":"10.1088/1758-5090/add37e","url":null,"abstract":"Organs-on-Chips (OoCs) are 3D models aiming to faithfully replicatein vitrospecific functions of human organs or tissues. While promising as an alternative to traditional 2D cell culture and animal models in drug development, controlled realization of complex microvasculature within OoC remains a significant challenge. Here, we demonstrate how femtosecond laser patterning allows to produce hollow microvascular-like channels inside a collagen-based matrix directly within a microfluidic chip. The hydrogel preparation protocol was optimized to maintain structural stability, facilitating successful endothelialization of produced channels. The resulting microvascular structures exhibit notable physiological relevance, as evidenced by the expression of key endothelial markers (ZO-1, and VE-cadherin) and the successful reproduction of the barrier function. Furthermore, tumor necrosis factor-alpha (TNF-α) exposure induces a concentration-dependent increase in vascular permeability and expression of intercellular adhesion molecule-1 (ICAM-1). The proposed method holds the potential to control and faithfully reproduce the vascularization process in OoC platforms, in both physiological and inflammatory conditions.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of small tissue engineered blood vessels and their clinical and research applications. 组织工程小血管的发展及其临床和研究应用。

IF 8.2 2区医学

Biofabrication Pub Date : 2025-05-27 DOI: 10.1088/1758-5090/add626

Elia Bosch-Rué, Qiao Zhang, George A Truskey, Jenifer Olmos Buitrago, Begoña M Bosch, Román A Pérez

引用次数: 0

Multifunctional nanoplatform based on polyethylene glycol-folic acid modified UiO-66 (Zr) as drug delivery platform for enhanced therapy of cancer. 基于聚乙二醇-叶酸修饰UiO-66 （Zr）的多功能纳米平台作为癌症强化治疗的药物传递平台。

IF 8.2 2区医学

Biofabrication Pub Date : 2025-05-27 DOI: 10.1088/1758-5090/add9d2

Mengyuan Li, Jiaming Ge, Jingwen Yao, Yuanhao Zhang, Lin Ma, Zheng Li, Xiangli Han, Ming Liu, Fei Tian, Jing Zhao

{"title":"Multifunctional nanoplatform based on polyethylene glycol-folic acid modified UiO-66 (Zr) as drug delivery platform for enhanced therapy of cancer.","authors":"Mengyuan Li, Jiaming Ge, Jingwen Yao, Yuanhao Zhang, Lin Ma, Zheng Li, Xiangli Han, Ming Liu, Fei Tian, Jing Zhao","doi":"10.1088/1758-5090/add9d2","DOIUrl":"10.1088/1758-5090/add9d2","url":null,"abstract":"Oral squamous cell carcinoma (OSCC) is the most common malignant tumor in the head and neck. Due to low bioavailability and passive targetability of anticancer drugs show great limitations in cancer therapy, the treatment of OSCC faces major challenges. Folic acid (FA) targeting can deliver anticancer drugs efficiently into the tumor environment, further enhance the anti-cancer efficacy. Herein, the nanoplatform based on UiO-66 that encapsulated with an effective FA targeting ligands and the pH-responsive polyethylene glycol (PEG) layer for the targeted delivery of berberine (Ber) is constructed for fighting against OSCC. The FA modification and controlled pH-responsiveness enable the targeted delivery of UiO-66/PEG-FA, which promotes the release of Ber and increases the cumulative intracellular Ber concentration, which both promote consumption of glutathione (GSH) and induced generation of reactive oxygen species (ROS), further stimulate the secretion of inflammatory factors (TNF-αand IL-1β). A comprehensive evaluation ofin vitroandin vivoexperiments show that UiO-66@Ber/PEG-FA promote autophagy and apoptosis of tumor cells by regulating the expression of Beclin-1, ATG13, BAX and Bcl-2, and effectively inhibit tumor growth. Overall, UiO-66@Ber/PEG-FA exhibit superior pH-responsiveness and targeted therapeutic efficienciesin vitroand vivo, it can serve as an approach for OSCC therapy.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D bioprinting of human iPSC-derived cardiac constructs with microvascular network support for improved graft survivalin vivo. 具有微血管网络支持的人类ipsc衍生心脏结构的3D生物打印，可提高移植物在体内的存活率。

IF 8.2 2区医学

Biofabrication Pub Date : 2025-05-23 DOI: 10.1088/1758-5090/add627

Léa Pourchet, Laura Casado-Medina, Yvonne Richaud-Patin, Karine Tadevosyan, Alba Morillas-García, Edgar Lorenzo, Ioannis Lazis, Antoni Ventura, Jagoda Litowczenko, Jordi Guiu, Angel Raya

{"title":"3D bioprinting of human iPSC-derived cardiac constructs with microvascular network support for improved graft survivalin vivo.","authors":"Léa Pourchet, Laura Casado-Medina, Yvonne Richaud-Patin, Karine Tadevosyan, Alba Morillas-García, Edgar Lorenzo, Ioannis Lazis, Antoni Ventura, Jagoda Litowczenko, Jordi Guiu, Angel Raya","doi":"10.1088/1758-5090/add627","DOIUrl":"10.1088/1758-5090/add627","url":null,"abstract":"Cardiac tissue engineering is a rapidly growing field that holds great promise for the development of new therapies for heart disease. While significant progress has been made in the field over the past two decades, engineering functional myocardium of clinically relevant size and thickness remains an unmet challenge. A major roadblock in this respect is the current difficulty in incorporating efficient vascularization into engineered constructs. One potential solution involves the use of microvascular fragments from adipose tissue, which have demonstrated encouraging results in improving vascularization and graft survival following transplantation. However, this method lacks precise control over the vascular architecture within the constructs. Here, we set out to investigate the use of 3D bioprinting for the fabrication of human cardiac tissue constructs composed of human induced pluripotent stem cell derivatives, while allowing for the precise control of the distribution and density of microvessel fragments within the bioprinted constructs. We carefully selected and optimized bioink compositions based on their printability, biocompatibility, and construct stability. Following transplantation into immunodeficient mice, 3D bioprinted cardiac constructs containing microvessel fragments exhibited rapid and efficient vascularization, resulting in prolonged graft survival. Overall, our studies underscore the advantages of employing engineering design and self-assembly across different scales to address current limitations of tissue engineering, and highlight the usefulness of 3D bioprinting in this context.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acoustic holographic assembly of cell-dense tissue constructs. 细胞致密组织结构的声学全息组装。

IF 8.2 2区医学

Biofabrication Pub Date : 2025-05-22 DOI: 10.1088/1758-5090/add49e

Minghui Shi, Peer Fischer, Kai Melde

{"title":"Acoustic holographic assembly of cell-dense tissue constructs.","authors":"Minghui Shi, Peer Fischer, Kai Melde","doi":"10.1088/1758-5090/add49e","DOIUrl":"10.1088/1758-5090/add49e","url":null,"abstract":"Tissue engineering aims to develop tissue constructs as models or substitutes for native tissues. For organ-level biological studies and regenerative medicine applications, it is essential to fabricate tissue constructs with physiologically relevant cell densities (on the order of 10 million to 1 billion cells·ml-1), large size (centimeter scale and larger), and a controllable geometry to guide tissue maturation. State-of-the-art biofabrication methods, however, struggle to simultaneously meet all of these demands. The recently proposed acoustic holographic assembly (AHA) method shows promise, as it is compatible with culture media and enables the contactless, label-free, and volumetric assembly of biological cells in a predefined geometry within few minutes. Here we present an AHA biofabrication scheme designated for fabricating cell-dense, centimeter-scale, and arbitrarily-shaped tissue constructs using a compact benchtop instrument compatible with a biolab environment. We demonstrate the assembly of C2C12 myoblasts in gelatin methacryloyl (GelMA) into large and asymmetric branch-shaped constructs, which are rapidly formed with an average cell density of 40 million cells·ml-1and a local density of up to 260 million cells·ml-1. Featuring a high viability of 90.5 ± 4.3%, the assembled cell constructs are observed to grow within the GelMA hydrogel under perfusion over five days. Further, we show how AHA can-in a single step-assemble cells into layered and three-dimensional geometries inside standard cell culture labware. It can therefore help obtain engineered tissue constructs with structural and functional characteristics seen in more complex native tissues.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regeneration of cartilage defects using engineered extracellular vesicles. 利用工程化细胞外囊泡再生软骨缺损。

IF 8.2 2区医学

Biofabrication Pub Date : 2025-05-22 DOI: 10.1088/1758-5090/addc41

Parisa Samadi, Mohsen Sheykhhasan, Ilham Omer, Asad Ullah, Ahmed Zarea, Victoria Toomajian, Muhammad Umar Aslam Khan, Derya Ertas, Lamont R Jones, Albert M Levin, Anwarul Hasan, Christopher H Contag, Yavuz Nuri Ertas, Nureddin Ashammakhi

{"title":"Regeneration of cartilage defects using engineered extracellular vesicles.","authors":"Parisa Samadi, Mohsen Sheykhhasan, Ilham Omer, Asad Ullah, Ahmed Zarea, Victoria Toomajian, Muhammad Umar Aslam Khan, Derya Ertas, Lamont R Jones, Albert M Levin, Anwarul Hasan, Christopher H Contag, Yavuz Nuri Ertas, Nureddin Ashammakhi","doi":"10.1088/1758-5090/addc41","DOIUrl":"https://doi.org/10.1088/1758-5090/addc41","url":null,"abstract":"In recent years, the number of adults with diagnosed cartilage defects has increased significantly, and various modes of treatment have been sought. However, traditional cartilage repair strategies have been proven inefficient, with limited success. Recently, regenerative treatment options have become more routinely used for specific indications, but they still have major limitations. Cell-derived extracellular vesicles (EVs) are becoming increasingly attractive for regenerative purposes because they provide several regenerative factors. In addition, they can be engineered to function as delivery agents for proteins, nucleic acids, and other molecules. Recently, EVs were explored for cartilage tissue engineering, with varying results. Unlike other cell-based therapies, this approach will lead to the avoidance of problems associated with immunogenic reactions against allogenic cells and easier approval of the therapy by regulatory bodies, which is expected to stimulate wider clinical application. Because of its broad interest and importance, this review was developed to discuss published works, their outcomes, and limitations and outline future research directions.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetically modified cell membrane proteins in tissue engineering and regenerative medicine. 基因修饰细胞膜蛋白在组织工程和再生医学中的应用。

IF 8.2 2区医学

Biofabrication Pub Date : 2025-05-19 DOI: 10.1088/1758-5090/add625

Yilin Bao, Yue Hu, Mengxuan Hao, Qinmeng Zhang, Guoli Yang, Zhiwei Jiang

引用次数: 0

Efficient wet-spinning of pre-aligned microtissues for 3D bioprinting complex tissue alignment. 用于3D生物打印复杂组织对齐的预对齐微组织的高效湿纺。

IF 8.2 2区医学

Biofabrication Pub Date : 2025-05-16 DOI: 10.1088/1758-5090/add37f

Caleb D Vogt, Joseph R Broomhead, Kyle Y Kunisaki, Johanna Margaret Teegarden, Kallie L Frett, Kyleigh Q Pacello, Anthony H Vitale, Angela Panoskaltsis-Mortari

{"title":"Efficient wet-spinning of pre-aligned microtissues for 3D bioprinting complex tissue alignment.","authors":"Caleb D Vogt, Joseph R Broomhead, Kyle Y Kunisaki, Johanna Margaret Teegarden, Kallie L Frett, Kyleigh Q Pacello, Anthony H Vitale, Angela Panoskaltsis-Mortari","doi":"10.1088/1758-5090/add37f","DOIUrl":"10.1088/1758-5090/add37f","url":null,"abstract":"Engineering functional smooth muscle tissues requires precise control of cellular alignment, particularly in complex anatomical regions such as the gastroesophageal junction (GEJ). We present a scalable wet-spinning approach for generating pre-aligned microtissues (PAMs) from immortalized human esophageal smooth muscle cells embedded in a collagen-alginate core-shell fiber. After maturation, fibers were sectioned into uniform PAMs with preserved alignment and high cell viability. Immunofluorescence and gene expression analyses confirmed the expression of key contractile markers. PAMs were incorporated into a gelatin-methacryloyl bioink and 3D bioprinted to demonstrate alignment along the extrusion path. This method does not require specialized culture platforms and enables efficient production of aligned microtissues for bioprinting. It offers a promising strategy for fabricating anisotropic tissues and may facilitate the reconstruction of complex muscle structures such as the GEJ.","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12083473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0