Biofabrication最新文献

{"title":"Advances in microfluidic biofabrication technology for bone metastasis modeling.","authors":"Mehdi Khanmohammadi, Nima Ahmadkhani, Marina Volpi, Khadijeh Khederlou, Alankrita Uppal, Mahdis Hosseini, Yu Shrike Zhang, Wojciech Swieszkowski","doi":"10.1088/1758-5090/add95f","DOIUrl":"10.1088/1758-5090/add95f","url":null,"abstract":"<p><p>Studying bone metastasis in<i>in vitro</i>models is essential for understanding the mechanisms driving this process, developing effective therapeutic strategies, and evaluating potential treatments for metastatic cancer patients. To this end, traditional two-dimensional (2D) cell culture models fail to replicate the native three-dimensional (3D) tissue microenvironment, resulting in significant disparities in biologically relevant behaviors and drug responses. The shift from 2D to 3D cell culture techniques represents an important step toward creating more biomimetic bone metastasis models. These systems more effectively emulate and replicate the complex interactions between cancer cells and bone tissue, including essential cell-cell and cell-extracellular matrix interactions, as well as<i>in vivo</i>biomechanical cues. The development and application of microfluidic-based 3D cancer models, incorporating diverse shapes, architectures, and modular structures such as organ-on-chip platforms, enable comprehensive screening and exploration of cellular interplay, the dissection of signaling pathways, and the resolution of limitations associated with traditional models. This review highlights recent advancements in microfluidic-based 3D bone metastasis models and examines innovative applications of this technology. These include hydrogel-based spherical and filaments biofabrication approaches, 2D and 3D tumor on-a- chips, and drug screening techniques such as concentration gradient generator-based, microdroplet-based, and microarray-based chips, as well as tumor tissue chips. Additionally, we discuss the benefits and limitations of these approaches in treating bone metastases and propose future directions for advancing microfluidic platforms in drug discovery and this research field.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic bioprinting as a tool to produce hiPSCs-derived renal organoids.","authors":"Chiara Formica, Gabriele Addario, Sveva Fagiolino, Lorenzo Moroni, Carlos Mota","doi":"10.1088/1758-5090/addb7e","DOIUrl":"10.1088/1758-5090/addb7e","url":null,"abstract":"<p><p>Chronic kidney disease affects 10% of the global population and often progresses to end-stage renal disease, where dialysis or renal transplant are the only therapies, though neither is a permanent solution. Regenerative medicine, particularly the use of organoids, offers a potential solution. Organoids are valuable for studying organ development, diseases, and regeneration, and are suitable for drug screening. However, their limited ability to replicate adult organs' maturation, complexity, and functions restricts their application. Additionally, manual production of organoids causes variability, affecting scalability and reproducibility. Automation techniques like bioprinting could enhance organoid maturation and complexity by depositing cells and biomaterials in a controlled manner. In this study, we established differentiation protocols to obtain human induced pluripotent stem cell-derived metanephric mesenchyme, ureteric bud progenitors, and the combination of these was used to form organoids. A microfluidic bioprinter capable of producing core-shell filaments was used to bioprint single cell progenitors in combination with gelatin in the core wrapped with an alginate shell. These filament constructs were cultured with an optimized mixture of growth factors for two weeks. Within one week, renal vesicles were visible, and after two weeks post-bioprinting the kidney organoids were functional and respond to the nephrotoxic drug doxorubicin. In conclusion, a bioprinted method was developed to generate in an automated way functional renal organoids from progenitors, offering a foundation for future kidney disease treatment.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofabrication in suspension media-a decade of advances.","authors":"Megan E Cooke, Morgan B Riffe, Manuela E Gomes, Rui M A Domingues, Jason A Burdick","doi":"10.1088/1758-5090/addc42","DOIUrl":"10.1088/1758-5090/addc42","url":null,"abstract":"<p><p>Suspension bath bioprinting, defined as extrusion bioprinting into a suspension bath consisting of a yield-stress material with fast recovery, emerged over a decade ago. Since this time, many suspension baths have been developed from molecular assemblies to granular media and across a range of synthetic and natural polymers. These suspension baths have been applied to the printing of a wide variety of inks for applications in tissue engineering, from<i>in vitro</i>tissue models to implantable constructs. In a scoping search of published literature over the past decade, 254 articles were identified that met various definitions related to suspension baths for biofabrication in order to gain a perspective on the various materials used and their applications; however, the literature is much more broad than this due to the disperse terminology that has been applied to the approach. This article gives a perspective on the progress that has been made in suspension bath printing, including applications of the technology and challenges that exist across the field, as well as provides a look to the future of where such printing methods will make an impact.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12131275/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofabrication of microstructured bacterial ecosystems using chaotic bioprinting: advancing<i>in vitro</i>research for microbial engineering.","authors":"Ariel Cantoral-Sánchez, Oscar Emmanuel Solís-Pérez, Francisco Javier Flores-Loera, Claudia Maribel Luna-Aguirre, Luis Fernando Carmona-Ramirez, Ilsa Pamela De Los Santos-Hernández, Nora Greys Zamora-Benavides, Mara Neher, Grissel Trujillo de Santiago, Mario Moisés Alvarez","doi":"10.1088/1758-5090/add568","DOIUrl":"10.1088/1758-5090/add568","url":null,"abstract":"<p><p>Mixed microbial communities are essential for various ecosystems, with bacteria often exhibiting unique behaviors in structured environments. However, replicating these interactions<i>in vitro</i>remains challenging, as traditional microbiology techniques based on well-mixed cultures fail to capture the spatial organization of natural communities. Chaotic 3D printing offers a versatile, high-throughput method for fabricating hydrogel constructs with multilayered microstructure in which different bacterial strains can coexist, closely mimicking the partial segregation seen in natural microbial ecosystems. Using a Kenics static mixer printing nozzle, we bioprinted a bacterial consortium consisting of<i>Lactobacillus rhamnosus, Bifidobacterium bifidum</i>, and<i>Escherichia coli</i>as a simplified model for human gut microbiota. Chaotic bioprinting enabled the creation of microstructured cocultures with distinct niches, allowing all bacterial strains to coexist (without being scrambled) and reach a population equilibrium. We characterized the cocultures through fluorescence microscopy, colony counting, and quantitative polymerase chain reactions. Our results demonstrate that the microarchitecture of the printed fibers significantly influences bacterial growth dynamics. Stratified arrangements enhanced coculture viability and balance over 72 h compared to well-mixed and suspension conditions. Chaotic printing also allows the rational arrangement of strict anaerobic bacteria, such as<i>B. bifidum</i>, by positioning them in construct layers that are more susceptible to hypoxia. Chaotic bioprinting presents a powerful tool for engineering microbial ecosystems with precise spatial control in the range of tens of micrometers. This approach promises to advance our understanding of microbial interactions and has potential biomedical applications in antibiotic testing, microbiota research, bioremediation, and synthetic biology.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Additive manufacturing of silicon nitride fiber-reinforced polyetheretherketone composites with enhanced mechanical strength and multifunctional bioactivity for load-bearing bone defect repair.","authors":"Shengxin Zeng, Haozheng Li, Panpan Hu, Zihe Li, Zhengguang Wang, Jiedong Wang, Jiasheng Chen, Shouzhan Wang, Gong Wang, Wei Zhao, Feng Wei","doi":"10.1088/1758-5090/add9d3","DOIUrl":"10.1088/1758-5090/add9d3","url":null,"abstract":"<p><p>Polyether ether ketone (PEEK) is increasingly applied in bone defect repair due to its excellent biocompatibility and absence of artifact formation. However, the bio-inertness and inadequate mechanical properties of untreated PEEK remain significant challenges for PEEK-based implants. Hence, this study prepares a series of silicon nitride (Si₃N₄) fiber-reinforced PEEK composite porous scaffolds using twin-screw melt mixing-extrusion and material extrusion 3D printing. Comprehensive evaluations assess the mechanical properties, biocompatibility, osteogenic differentiation, angiogenesis activities, and antibacterial performances of various composites. Characterization results show that Si₃N₄fiber-reinforced PEEK composites exhibit excellent printability, with well-oriented Si₃N₄fibers uniformly distributed throughout the matrix. Furthermore, compared to non-reinforced PEEK, the addition of 8% Si₃N₄fibers enhanced Young's modulus by 52.2% (6.36 GPa). Additionally, both<i>in vitro</i>and<i>in vivo</i>results indicate that all composite scaffolds exhibit excellent biocompatibility. Notably, the 8% Si₃N₄fiber-reinforced PEEK composite demonstrated optimal multifunctional performance in osteogenic induction, angiogenic capacity, and antibacterial efficacy, significantly outperforming other experimental groups. In conclusion, this study offers a solution for enhancing the mechanical, anti-infective, and osseointegrative properties of PEEK, demonstrating its great potential for expanding the application of non-metallic orthopedic implants in bone defect repair.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High resolution melt electro-written scaffolds promote alignment of human skeletal muscle cells.","authors":"Finn Snow, Cathal O'Connell, Aaron Elbourne, Magdalena Kita, Peiqi Yang, Richard J Williams, Simon E Moulton, Elena Pirogova, Robert Michail Ivan Kapsa, Anita Quigley","doi":"10.1088/1758-5090/add960","DOIUrl":"10.1088/1758-5090/add960","url":null,"abstract":"<p><p>Advanced tissue engineering (TE) strategies are vital to address challenging musculoskeletal conditions, such as volumetric muscle loss. These disorders impose a considerable economic burden and affect individuals' quality of life, highlighting the need for innovative treatments, such as TE, to address these challenges. Here, we examine how scaffold fibre orientation influences mechanical properties and cellular behaviour by utilising melt electrowriting (MEW) as a high-resolution 3D printing technique that combines aspects of electrospinning and melt based polymer deposition. In this work, we investigated the effects of fibre orientation in MEW scaffolds, and its effect on the scaffold mechanical properties as well as cell orientation and alignment. MEW scaffolds were mechanically characterised through uniaxial strain testing to determine critical parameters, including strain at failure, ultimate tensile strength, Young's modulus (<i>E</i>), fatigue rate, recovery time, and yield strain. These mechanical properties were analysed to define an optimal strain regime for transitioning from static to dynamic culture conditions under muscle-like cyclic loading, relevant to muscle's viscoelastic behaviour. In parallel, static cultures of primary human skeletal muscle myoblasts and normal human dermal fibroblasts (NHDFs) were grown on MEW scaffolds, with varying architectures, to study the effects of fibre aspect ratio on cell alignment. Cell alignment was visualised using DAPI/phalloidin staining and quantified with the ImageJ directionality plugin, enabling a systematic comparison of scaffold designs. This approach evaluates the potential of supportive scaffold architectures to promote aligned cell growth, offering insights into designing effective scaffolds for tissue regeneration.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlled microvasculature for organ-on-a-chip applications produced by high-definition laser patterning.","authors":"Alice Salvadori, Masafumi Watanabe, Marica Markovic, Ryo Sudo, Aleksandr Ovsianikov","doi":"10.1088/1758-5090/add37e","DOIUrl":"10.1088/1758-5090/add37e","url":null,"abstract":"<p><p>Organs-on-Chips (OoCs) are 3D models aiming to faithfully replicate<i>in vitro</i>specific functions of human organs or tissues. While promising as an alternative to traditional 2D cell culture and animal models in drug development, controlled realization of complex microvasculature within OoC remains a significant challenge. Here, we demonstrate how femtosecond laser patterning allows to produce hollow microvascular-like channels inside a collagen-based matrix directly within a microfluidic chip. The hydrogel preparation protocol was optimized to maintain structural stability, facilitating successful endothelialization of produced channels. The resulting microvascular structures exhibit notable physiological relevance, as evidenced by the expression of key endothelial markers (ZO-1, and VE-cadherin) and the successful reproduction of the barrier function. Furthermore, tumor necrosis factor-alpha (TNF-α) exposure induces a concentration-dependent increase in vascular permeability and expression of intercellular adhesion molecule-1 (ICAM-1). The proposed method holds the potential to control and faithfully reproduce the vascularization process in OoC platforms, in both physiological and inflammatory conditions.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of small tissue engineered blood vessels and their clinical and research applications.","authors":"Elia Bosch-Rué, Qiao Zhang, George A Truskey, Jenifer Olmos Buitrago, Begoña M Bosch, Román A Pérez","doi":"10.1088/1758-5090/add626","DOIUrl":"10.1088/1758-5090/add626","url":null,"abstract":"<p><p>Since the first tissue engineered blood vessel (TEBV) was developed, different approaches, biomaterial scaffolds and cell sources have been used to obtain an engineered vessel as much similar as native vessels in terms of structure, functionality and mechanical properties. At the same time, diverse needs to obtain a functional TEBV have emerged, such as for blood vessel replacement for cardiovascular diseases (CVDs) to be used as artery bypass, to vascularize tissue engineered constructs, or even to model vascular diseases or drug testing. In this review, after briefly describing the native structure and function of arteries, we will give an overview of different biomaterials, cells and methods that have been used during the last years for the development of small TEBV (1-6 mm diameter). The importance of perfusing the TEBV to acquire functionality and maturation will be also discussed. Finally, we will center the review on TEBV applications beyond their use as vascular graft for CVDs.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional nanoplatform based on polyethylene glycol-folic acid modified UiO-66 (Zr) as drug delivery platform for enhanced therapy of cancer.","authors":"Mengyuan Li, Jiaming Ge, Jingwen Yao, Yuanhao Zhang, Lin Ma, Zheng Li, Xiangli Han, Ming Liu, Fei Tian, Jing Zhao","doi":"10.1088/1758-5090/add9d2","DOIUrl":"10.1088/1758-5090/add9d2","url":null,"abstract":"<p><p>Oral squamous cell carcinoma (OSCC) is the most common malignant tumor in the head and neck. Due to low bioavailability and passive targetability of anticancer drugs show great limitations in cancer therapy, the treatment of OSCC faces major challenges. Folic acid (FA) targeting can deliver anticancer drugs efficiently into the tumor environment, further enhance the anti-cancer efficacy. Herein, the nanoplatform based on UiO-66 that encapsulated with an effective FA targeting ligands and the pH-responsive polyethylene glycol (PEG) layer for the targeted delivery of berberine (Ber) is constructed for fighting against OSCC. The FA modification and controlled pH-responsiveness enable the targeted delivery of UiO-66/PEG-FA, which promotes the release of Ber and increases the cumulative intracellular Ber concentration, which both promote consumption of glutathione (GSH) and induced generation of reactive oxygen species (ROS), further stimulate the secretion of inflammatory factors (TNF-<i>α</i>and IL-1<i>β</i>). A comprehensive evaluation of<i>in vitro</i>and<i>in vivo</i>experiments show that UiO-66@Ber/PEG-FA promote autophagy and apoptosis of tumor cells by regulating the expression of Beclin-1, ATG13, BAX and Bcl-2, and effectively inhibit tumor growth. Overall, UiO-66@Ber/PEG-FA exhibit superior pH-responsiveness and targeted therapeutic efficiencies<i>in vitro</i>and vivo, it can serve as an approach for OSCC therapy.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D bioprinting of human iPSC-derived cardiac constructs with microvascular network support for improved graft survival<i>in vivo</i>.","authors":"Léa Pourchet, Laura Casado-Medina, Yvonne Richaud-Patin, Karine Tadevosyan, Alba Morillas-García, Edgar Lorenzo, Ioannis Lazis, Antoni Ventura, Jagoda Litowczenko, Jordi Guiu, Angel Raya","doi":"10.1088/1758-5090/add627","DOIUrl":"10.1088/1758-5090/add627","url":null,"abstract":"<p><p>Cardiac tissue engineering is a rapidly growing field that holds great promise for the development of new therapies for heart disease. While significant progress has been made in the field over the past two decades, engineering functional myocardium of clinically relevant size and thickness remains an unmet challenge. A major roadblock in this respect is the current difficulty in incorporating efficient vascularization into engineered constructs. One potential solution involves the use of microvascular fragments from adipose tissue, which have demonstrated encouraging results in improving vascularization and graft survival following transplantation. However, this method lacks precise control over the vascular architecture within the constructs. Here, we set out to investigate the use of 3D bioprinting for the fabrication of human cardiac tissue constructs composed of human induced pluripotent stem cell derivatives, while allowing for the precise control of the distribution and density of microvessel fragments within the bioprinted constructs. We carefully selected and optimized bioink compositions based on their printability, biocompatibility, and construct stability. Following transplantation into immunodeficient mice, 3D bioprinted cardiac constructs containing microvessel fragments exhibited rapid and efficient vascularization, resulting in prolonged graft survival. Overall, our studies underscore the advantages of employing engineering design and self-assembly across different scales to address current limitations of tissue engineering, and highlight the usefulness of 3D bioprinting in this context.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}