Biofabrication最新文献

{"title":"Corrigendum: Toward a neurospheroid niche model: optimizing embedded 3D bioprinting for fabrication of neurospheroid brain-like co-culture constructs (2021<i>Biofabrication</i>13 015014).","authors":"Yi-Chen Ethan Li, Yasamin A Jodat, Roya Samanipour, Giulio Zorzi, Kai Zhu, Minoru Hirano, Karen Chang, Adnan Arnaout, Shabir Hassan, Navneet Matharu, Ali Khademhosseini, Mina Hoorfar, Su Ryon Shin","doi":"10.1088/1758-5090/adb74a","DOIUrl":"https://doi.org/10.1088/1758-5090/adb74a","url":null,"abstract":"","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":"17 2","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellnet technology to generate 3D, functional, single-cell networks in custom architectures within collagen.","authors":"Arun Poudel, Puskal Kunwar, Ujjwal Aryal, Anna-Blessing Merife, Pranav Soman","doi":"10.1088/1758-5090/adc48f","DOIUrl":"10.1088/1758-5090/adc48f","url":null,"abstract":"<p><p>Cells possess the remarkable ability to generate tissue-specific 3D interconnected networks and respond to a wide range of stimuli. Understanding the link between the spatial arrangement of individual cells and their networks' emergent properties is necessary for the discovery of both fundamental biology as well as applied therapeutics. However, current methods spanning from lithography to 3D photo-patterning to acoustofluidic devices are unable to generate interconnected and organized single cell 3D networks within native extracellular matrix (ECM). To address this challenge, we report a novel technology coined as Cellnet. This involves the use of natural collagen crosslinked within three-chambered microfluidic chips followed by femtosecond laser-assisted cavitation to generate user-defined 3D microchannel networks. Model cells, seeded within side chamber of the chip, migrate within microchannel networks within hours, self-organize and form viable, interconnected, 3D single-cell networks in custom architectures such as square grid, concentric circle, parallel lines, and spiral patterns. Heterotypic Cellnets can also be generated by seeding multiple cell types in side-chambers of the chip. The functionality of cell networks can be studied by monitoring the real-time calcium signaling response of individual cells and signal propagation within Cellnets when subjected to flow stimulus alone or a sequential combination of flow and biochemical stimuli. Furthermore, user-defined disrupted Cellnets can be generated by lethally injuring target cells within the 3D network and analyzing the changes in their signaling dynamics. As compared to the current self-assembly based methods that exhibit high variability and poor reproducibility, Cellnets can generate organized 3D single-cell networks and their real-time signaling responses to a range of stimuli can be accurately captured using simple cell seeding and easy-to-handle microfluidic chips. Cellnet technology, agnostic of cell types, ECM formulations, 3D cell-connectivity designs, or location and timing of network disruptions, could pave the way to address a range of fundamental and applied bioscience applications.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A hollow fiber membrane-based liver organoid-on-a-chip model for examining drug metabolism and transport.","authors":"Adam Myszczyszyn, Anna Muench, Vivian Lehmann, Theo Sinnige, Frank G van Steenbeek, Manon Bouwmeester, Roos-Anne Samsom, Marit Keuper-Navis, Thomas K van der Made, Daniel Kogan, Sarah Braem, Luc J W van der Laan, Hossein Eslami Amirabadi, Evita van de Steeg, Rosalinde Masereeuw, Bart Spee","doi":"10.1088/1758-5090/adc3ce","DOIUrl":"10.1088/1758-5090/adc3ce","url":null,"abstract":"<p><p>Liver-on-a-chip models predictive for both metabolism, and blood and canalicular transport of drug candidates in humans are lacking. Here, we established a bioengineered and 3Rs-complied (animal component-free) hepatocyte-like millifluidic system based on 3D hollow fiber membranes (HFMs), recombinant human laminin 332 coating and adult human stem cell-derived organoids. Organoid fragments formed polarized and tight monolayers on HFMs with improved hepatocyte-like maturation, as compared to standard 3D organoid cultures in Matrigel from matched donors. Gene expression profiling and immunofluorescence revealed that hepatocyte-like monolayers expressed a broad panel of phase I (e.g. CYP3A4, CYP2D6, CYP2C9) and II (e.g. UGTs, SULTs) drug-metabolizing enzymes and drug transporters (e.g. MDR1, MRP3, OATP1B3). Moreover, statically cultured monolayers displayed phase I and II metabolism of a cocktail of six relevant compounds, including midazolam and 7-hydroxycoumarin. We also demonstrated the disposition of midazolam in the basal/blood-like circulation and apical/canalicular-like compartment of the millifluidic chip. Finally, we studied the bioavailability of midazolam and coumarin on-a-chip in combination with a small intestine-like system. In conclusion, we generated a proof-of-concept liver organoid-on-a-chip model for examining metabolism and transport of drugs, which can be further developed to predict pharmacokinetics' (PK)/absorption, distribution, metabolism and excretion (ADME) profiles in humans.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering strategies for the construction of oriented and functional skeletal muscle tissues.","authors":"Tingting Fan, Minxuan Jia, Heng Liu, Zili Gao, Wenhui Huang, Wenli Liu, Qi Gu","doi":"10.1088/1758-5090/adbfc2","DOIUrl":"10.1088/1758-5090/adbfc2","url":null,"abstract":"<p><p>The growth and formation of tissues, such as skeletal muscle, involve a complex interplay of spatiotemporal events, including cell migration, orientation, proliferation, and differentiation. With the continuous advancement of<i>in vitro</i>construction techniques, many studies have contributed to skeletal muscle tissue engineering (STME). This review summarizes recent advances in the ordered construction of skeletal muscle tissues, and evaluates the impact of engineering strategies on cell behavior and maturation, including biomaterials, manufacturing methods and training means. Biomaterials are used as scaffolds to provide a good microenvironment for myoblasts, manufacturing methods to guide the alignment of myoblasts through construction techniques, and external stimulation to further promote the myoblast orientation and maturation after construction, resulting in oriented and functional skeletal muscle tissues. Subsequently, we critically examine recent advancements in engineered composite skeletal muscle constructs, with particular emphasis on essential functionalization strategies including skeletal muscle vascularization, innervation and others. Concurrently, we evaluate emerging applications of STME in diverse translational areas such as volumetric muscle loss treatment, muscle-related disease models, drug screening, biohybrid robots, and cultured meat. Finally, future perspectives are proposed to provide guidance for rational design based on engineering strategies in STME.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-based multi-material bioprinting of vascularised adipose tissue for breast fatty tissue engineering.","authors":"Nina Hedemann, Alexander Thomas, Nils Tribian, Anna-Klara Amler, Sandra Krüger, David Holthaus, Patricia Huebbe, Inken Flörkemeier, Jörg Weimer, Nicolai Maass, Lutz Kloke, Dirk Bauerschlag, Marion Tina van Mackelenbergh","doi":"10.1088/1758-5090/adb890","DOIUrl":"10.1088/1758-5090/adb890","url":null,"abstract":"<p><p>Reconstructive surgery following breast cancer ablation is a surgical gold standard, but current options comprising autologous fatty tissue transfer and artificial soft tissue implants are inferior. With the advent of powerful biofabrication technologies, researchers for the first time have the tools to engineer life-like tissues with the ultimate goal of clinical application. Here, we apply multi-material stereolithographic bioprinting together with a novel sacrificial biomaterial system to engineer complex fatty tissue constructs. Biomaterials, cellular composition and cultivation conditions of these constructs were designed to enable<i>in vitro</i>creation of vascularised fatty tissue. Cells within the constructs showed an overall good survival (>93%), indicated by live-dead cell staining, over the entire cultivation period of 27 d. Adipose-derived stem cells were successfully differentiated<i>in situ</i>, forming fat vesicles and expressing adipocyte markers PPARγ, FAPB4 and S100B. Additionally, secretion of adipokines leptin and adiponectin into culture supernatants increased significantly. Endothelial cells vascularised the constructs, creating macro- and microvascular structures within the printed channels and extending beyond with culture time. Moreover, cells invaded into the surrounding hydrogel. The engineered fatty tissue constructs could serve as a base to develop patient-specific tissue building blocks with the final goal to achieve an all-natural reconstruction of the breast.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D bioprinting technology for modeling vascular diseases and its application.","authors":"Ju-El Kim, Gun-Jae Jeong, Young Min Yoo, Suk Ho Bhang, Jae Hoon Kim, Young Min Shin, Kyung Hyun Yoo, Byung-Chul Lee, Wooyeol Baek, Dong Nyoung Heo, Rosaire Mongrain, Jung Bok Lee, Jeong-Kee Yoon","doi":"10.1088/1758-5090/adc03a","DOIUrl":"10.1088/1758-5090/adc03a","url":null,"abstract":"<p><p><i>In vitro</i>modeling of vascular diseases provides a useful platform for drug screening and mechanistic studies, by recapitulating the essential structures and physiological characteristics of the native tissue. Bioprinting is an emerging technique that offers high-resolution 3D capabilities, which have recently been employed in the modeling of various tissues and associated diseases. Blood vessels are composed of multiple layers of distinct cell types, and experience different mechanical conditions depending on the vessel type. The intimal layer, in particular, is directly exposed to such hemodynamic conditions inducing shear stress, which in turn influence vascular physiology. 3D bioprinting techniques have addressed the structural limitations of the previous vascular models, by incorporating supporting cells such as smooth muscle cells, geometrical properties such as dilation, curvature, or branching, or mechanical stimulation such as shear stress and pulsatile pressure. This paper presents a review of the physiology of blood vessels along with the pathophysiology of the target diseases including atherosclerosis, thrombosis, aneurysms, and tumor angiogenesis. Additionally, it discusses recent advances in fabricating<i>in vitro</i>3D vascular disease models utilizing bioprinting techniques, while addressing the current challenges and future perspectives for the potential clinical translation into therapeutic interventions.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional 4D printed shape memory composite scaffolds with photothermal and magnetothermal effects for multimodal tumor therapy and bone repair.","authors":"Jingguang Wang, Jielong Zhou, Zhenze Xie, Yunhui Zhang, Muye He, Tianyu Wei, Shibin Wu, Chang Du","doi":"10.1088/1758-5090/adc29e","DOIUrl":"10.1088/1758-5090/adc29e","url":null,"abstract":"<p><p>Tumor recurrence and bone defects are two key challenges in the surgical treatment of osteosarcoma (OS). Therefore, it is highly necessary to develop a multifunctional scaffold that can simultaneously eradicate tumor cells and promote bone regeneration. Herein, a hierarchically porous shape memory scaffold consisting of hydroxyapatite, silica, poly(D,L-lactide-co-trimethylene carbonate) and Fe₃O₄(HSP-Fe₃O₄) is constructed by Pickering emulsion and 4D printing technique. The HSP-Fe₃O₄scaffold demonstrates the advantages of multimodal anti-tumor therapy, including chemotherapy through the Fenton reaction, effective photothermal conversion for photothermal therapy under near-infrared laser irradiation, and magnetothermal therapy provided by an alternating magnetic field. Furthermore, photothermal hyperthermia also serve as triggers for the shape memory effect of the HSP-Fe₃O₄scaffold, enabling the scaffold to precise adaptation of complex bone defects after minimally invasive surgical implantation. Additionally, the HSP-Fe₃O₄scaffold with interconnected multiscale pore exhibits good biocompatibility and excellent bone repair capabilities. This study proved that the HSP-Fe₃O₄scaffold provides positive insights for preventing tumor recurrence and facilitating bone regeneration after OS surgery.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced gelatin methacryloyl nanohydroxyapatite hydrogel for high-fidelity 3D printing of bone tissue engineering scaffolds.","authors":"Toufik Naolou, Nadine Schadzek, Jan Mathis Hornbostel, Iliyana Pepelanova, Miriam Frommer, Franziska Lötz, Leopold Sauheitl, Stefan Dultz, Vincent J M N L Felde, Ola Myklebost, Cornelia Lee-Thedieck","doi":"10.1088/1758-5090/adbb90","DOIUrl":"10.1088/1758-5090/adbb90","url":null,"abstract":"<p><p>Patients suffering from large bone defects are in urgent need of suitable bone replacements. Besides biocompatibility, such replacements need to mimic the 3D architecture of bone and match chemical, mechanical and biological properties, ideally promoting ossification. As natural bone mainly contains collagen type I and carbonate hydroxyapatite, a 3D-printable biomaterial consisting of methacrylated gelatin (GelMA) and nanohydroxyapatite (nHAp) would be beneficial to mimic the composition and shape of natural bone. So far, such nanocomposite hydrogels (NCH) suffered from unsatisfactory rheological properties making them unsuitable for extrusion-based 3D printing with high structural fidelity. In this study, we introduce a novel GelMA/nHAp NCH composition, incorporating the rheological modifier carbomer to improve rheological properties and addressing the challenge of calcium cations released from nHAp that hinder GelMA gelation. Leveraging its shear-thinning and self-healing properties, the NCH ink retains its shape and forms cohesive structures after deposition, which can be permanently stabilized by subsequent UV crosslinking. Consequently, the NCH enables the printing of 3D structures with high shape fidelity in all dimensions, including the<i>z</i>-direction, allowing the fabrication of highly macroporous constructs. Both the uncured and the UV crosslinked NCH behave like a viscoelastic solid, with<i>G</i>'><i>G</i>″ at deformations up to 100-200 %. After UV crosslinking, the NCH can, depending on the GelMA concentration, reach storage moduli of approximately 10 to over 100 kPa and a mean Young's Modulus of about 70 kPa. The printed scaffolds permit not only cell survival but also osteogenic differentiation, highlighting their potential for bone tissue engineering.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A self-assembling peptide-based hydrogel containing NF-<i>κ</i>B inhibitors and NGF for peripheral nerve injury repair.","authors":"HaiTao Su, QiuPing Ye, Di Wang, AnQi Liu, YaGuang Wang, Ye Zhang, LiJun Weng","doi":"10.1088/1758-5090/adc340","DOIUrl":"10.1088/1758-5090/adc340","url":null,"abstract":"<p><p>An inflammatory response may be initiated after peripheral nerve injury (PNI), potentially hindering the repair and regeneration of damaged nerves. Administering anti-inflammatory agents to modulate macrophage phenotypes may reduce post-injury inflammation and show potential for treating PNI. Regrettably, the limited half-lives of these compounds within the human body constrain their efficacy as anti-inflammatory agents. In this study, we co-assembled picroside II (PII) and nerve growth factor (NGF) with the hydrogelator compound Nap-Phe-Phe-Tyr-OH (NapFFY) to form a supramolecular hydrogel, PII/NGF/NapFFY@Gel, which could be accurately delivered to the nerve injury site via<i>in situ</i>injection to improve its bioavailability. Our results demonstrated that the PII/NGF/NapFFY@Gel exhibits favorable drug slow-release performance in both<i>in vivo</i>and<i>in vitro</i>experiments. Furthermore, cell and animal studies revealed that the PII/NGF/NapFFY@Gel effectively enhanced nerve recovery and regeneration by modulating the inflammatory microenvironment. This mechanism involves inhibiting the NF-<i>κ</i>B inflammatory signaling pathway, suppressing macrophage polarization to the M1 phenotype, and upregulating the expression of proteins associated with nerve regeneration. Taken together, the results of this study suggest that improving the inflammatory microenvironment and promoting nerve repair through the<i>in situ</i>injection of PII/NGF/NapFFY@Gel with sustained drug release may be a novel treatment for PNI.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":"17 2","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient one-step immobilization of DNA probes on 1DZnO nanoplatforms targeting a low-mutation region of SARS-CoV-2.","authors":"Shirlley Martínez Tolibia, Rafael A Salinas, Cesar Millán-Pacheco, Edgar O Castrejón González, Erik A Vázquez-Montelongo, Josué E Romero, Guillermo Santana, Ateet Dutt","doi":"10.1088/1758-5090/adc159","DOIUrl":"10.1088/1758-5090/adc159","url":null,"abstract":"<p><p>Fabricating cost-effective biosensors with rapid response times is highly desirable during pandemic scenarios, where accuracy, swift detection, and portability are crucial for making prompt decisions. The design and conceptualization of these devices at early stages are critical for enhancing their output responses. In this work, we implemented a one-step immobilization strategy for DNA probes targeting a low-mutation region from the envelope protein of SARS-CoV-2 onto one-dimensional ZnO nanostructures (1DZnO) to achieve high detection efficiency. First, DNA probes were designed to select a highly conserved region (L28-A36) among SARS-CoV-2 subvariants using bioinformatic analysis. Then, dynamic simulations were performed to estimate the binding affinity of DNA to 1DZnO, where phosphate molecules were identified as the functional groups with the highest affinity to the ZnO surface, followed by the sugar rings and the base pairs. In addition, linear interaction energies and their average contributions were calculated for the ssDNA/ZnO interfaces. Computational simulations were correlated to experimental techniques, where suitable DNA immobilization and target detection were confirmed by FTIR, photoluminescence (PL), transmission electron microscopy, and elemental mapping, corroborating the adsorption of DNA across the entire 1DZnO surface. Intense peaks related to C-C, C=C, C=N, P-O, and N-H were identified as the most important by FTIR characterizations, whereas PL showed a distinctive shift in deep level emission band between 520-530 nm, with a partial quenching of the near band emission signal, obtaining as well variations in the calculated bandgap. In summary, it is suggested that structural oxygen vacancies of 1DZnO nanoplatforms provide a significant proportion of active available sites for an easy and strong interaction with the phosphate backbone of DNA, enhancing physical adsorption. Furthermore, molecular validation by PCR confirmed the long-term stability of immobilized DNA probes, probing their suitability for further biosensing devices.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}