BiofabricationPub Date : 2025-07-24DOI: 10.1088/1758-5090/adeecc
Yan Li, Zhenyu Wu, Yan Zhou, Sedrati Manar, Rui Wang, Guohua Jiang
{"title":"Review on engineered polymer microneedles for drug delivery and disease diagnosis.","authors":"Yan Li, Zhenyu Wu, Yan Zhou, Sedrati Manar, Rui Wang, Guohua Jiang","doi":"10.1088/1758-5090/adeecc","DOIUrl":"10.1088/1758-5090/adeecc","url":null,"abstract":"<p><p>The minimally invasive and painless microneedle (MN) technology has become a promising platform for drug delivery and disease diagnosis. In this review, we first introduce the classification of MNs according to their sources and then summarize the preparation methods of MNs, including the stretching method, droplet-born air blowing, micromolding method, and 3D printing method. Subsequently, we also introduce how to prepare different types of MNs, such as solid, coated, hollow, dissolving, and frozen MNs, through material structure design. More importantly, the development of MNs in drug delivery, biosensing, wearable devices, cancer therapy and tissue regeneration in recent years has been reviewed. Finally, several significant challenges for further exploration in the field of MNs as well as perspectives and outlooks on future MN research, are also discussed in this review.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144616126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiofabricationPub Date : 2025-07-24DOI: 10.1088/1758-5090/adef81
Sean O Mathew, Ronghui Qi, Brian G Amsden
{"title":"Thermally stable, photocrossinkable and biocompatible copolymers for melt electrowriting.","authors":"Sean O Mathew, Ronghui Qi, Brian G Amsden","doi":"10.1088/1758-5090/adef81","DOIUrl":"10.1088/1758-5090/adef81","url":null,"abstract":"<p><p>Melt electrowriting (MEW) is capable of generating highly defined microarchitectures suitable for tissue engineering applications. The main biodegradable polymer typically utilized for MEW processing, poly(<i>ϵ</i>-caprolactone), is prone to creep under dynamic loads and plasticization due to water absorption, making its use problematic for situations demanding dynamic loading in aqueous media. Photocrosslinking during processing can eliminate these problems while also allowing for manipulation of mechanical properties. However, photocrosslinking strategies utilized to date have either limited processing time or require prolonged UV irradiation. Herein we demonstrate the potential of a cyclic trimethylene carbonate monomer bearing a pendant coumarin moiety (MUM) for creating MEW processable copolymers that are thermally stable and photocrosslinkable. The MUM was copolymerized with caprolactone to form copolymers that were MEW processed into both linear and crimped fiber structures followed by long-wave UV photocrosslinking yielding high modulus scaffolds with very low sol content. The photocrosslinked scaffolds were also cytocompatible. The ability to copolymerize MUM with other cyclic lactone monomers allows for the generation of a variety of MEW processable polymers with tunable properties. Collectively, the findings demonstrate the potential of MUM containing copolymers for MEW generation of scaffolds for a range of tissue engineering applications.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Near-infrared light and magnetic field dual-responsive 3D printed scaffolds for sequential treatment of infected bone defects.","authors":"Dapeng Zeng, Hao Wang, Zehao Yu, Xiaohan Mei, Boda Ying, Si Pu, Shibo Liu, Xiangjun Pan, Shicheng Zhou, Ruiyan Li, Yanguo Qin","doi":"10.1088/1758-5090/adebb3","DOIUrl":"10.1088/1758-5090/adebb3","url":null,"abstract":"<p><p>The treatment of infected bone defects remains a challenge due to the complex biological processes involved, including antibacterial, anti-inflammatory, angiogenesis and bone regeneration. Polyetherimide (PEI) has promising applications in orthopaedics, but its biological inertness limits its clinical efficacy. In this study, a smart near-infrared (NIR) light and magnetic field responsive 3D printed scaffold was developed by combining PEI and Fe<sub>3</sub>O<sub>4</sub>nanoparticles. Gelatin methacrylate hydrogel containing aloe-emodin (AE), a natural antimicrobial and antioxidant compound, was subsequently injected into the 3D printed scaffold to create the P-Fe<sub>3</sub>O<sub>4</sub>@GM-AE composite scaffold. This composite scaffold exhibited several key functionalities: Firstly, it effectively eliminated methicillin-resistant<i>Staphylococcus aureus</i>when exposed to NIR light, achieving an<i>in vivo</i>antimicrobial rate of 99.97 ± 0.1%. Secondly, it effectively removed reactive oxygen species and prevented the pro-inflammatory M1 polarization of macrophages in the infected bone defect microenvironment, creating favorable conditions for bone reconstruction. Moreover, during the reconstruction stage, the magnetic composite scaffold, when combined with a static magnetic field, promoted osteogenesis-angiogenesis coupling, thereby accelerating bone repair. Thus, this study provides new insights and methods for the sequential treatment of infected bone defects.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biohybrid microstructured hydrogels obtained via<i>in situ</i>extracellular matrix deposition and decellularization using supercritical CO<sub>2</sub>.","authors":"Vanessa Morais Lima, Albane Carré, Emmanuelle Poque, Maria-Dimitra Chiotelli, Natan Wiele, Christelle Harscoat-Schiavo, Raphaëlle Savoire, Teresa Simon-Yarza","doi":"10.1088/1758-5090/adebb4","DOIUrl":"10.1088/1758-5090/adebb4","url":null,"abstract":"<p><p>In recent decades, our understanding of biomaterials has shifted from seeing them simply as physical supports for cells or drug delivery platforms to recognizing their active and dynamic role in tissue repair, guided by their physicochemical, mechanical, and biological properties. Biologically derived materials such as the decellularized extracellular matrix (dECM) offer the advantage of replicating the biomolecular cellular environment and have been proposed for tissue regeneration. However, their use as scaffolds is hindered by poor mechanical properties and limited tunability of physical features. Herein, we fabricated a bioinspired hybrid hydrogel by integrating a chemically cross-linked microporous polysaccharide scaffold with native ECM directly secreted by cells. First, the scaffold synthesis and culture conditions were optimized to enhance ECM deposition by fibroblasts. To obtain an acellular scaffold, decellularization using supercritical CO<sub>2</sub>was performed and compared to a conventional method, demonstrating its superiority in ensuring efficient decellularization while preserving an enriched ECM lining the surface of the pores and preventing scaffold damage. The biohybrid hydrogel was characterized by a very low amount of DNA (<5 ng DNA mg<sup>-1</sup>) and a network of highly interconnected pores covered by an abundant ECM including collagen I, collagen IV, fibronectin, elastin and laminin. This work presents a new versatile strategy that can be adapted to various tissues to engineer biomimetic microstructured materials overcoming the limitations associated with polymer-based and dECM-based strategies when used independently.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Osteochondral organoid biofabrication: construction strategies, applications and perspectives.","authors":"Liwei Fu, Jiang Wu, Zhichao Zhang, Zhixing Zhang, Yazhe Zheng, Li Pinxue, Chuanyang Long, Xiang Sui, Shuyun Liu, Quanyi Guo","doi":"10.1088/1758-5090/ade740","DOIUrl":"10.1088/1758-5090/ade740","url":null,"abstract":"<p><p>Osteochondral tissue is a functional complex with crosstalk shown to occur between cartilage and subchondral bone, playing a pivotal role in joint function and mobility. Osteochondral tissue repair has long been an enormous challenge in regenerative medicine and tissue engineering. With the development of biofabrication and biomaterials innovations, organoid technology, which can mimic the biological architecture and characteristics of organs through the construction of 3D tissue structures<i>in vitro</i>, provides novel insight into osteochondral (OC) tissue regeneration. This review explores the significance of OC organoid biofabrication and the related biological structures and functions of the joint OC unit. Furthermore, we summarize novel biofabrication technologies used for OC organoids, such as 3D printing and microfluidics, and propose construction strategies for OC organoids. Finally, the application directions and challenges of OC organoids are outlined, emphasizing their potential for OC disease treatment.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"From microtissues to organs: the future of reconstructive surgery with organ building block-based bioprinting.","authors":"Qiumei Ji, Ruize Tang, Xingran Liu, Jing Yang, Xiangqi Liu, Qingfeng Li, Ru-Lin Huang","doi":"10.1088/1758-5090/aded37","DOIUrl":"10.1088/1758-5090/aded37","url":null,"abstract":"<p><p>Reconstructive surgery seeks to restore the aesthetic appearance and functional integrity of damaged organs and tissues. However, traditional approaches are fundamentally constrained by donor tissue scarcity and associated morbidity, highlighting the urgent need for engineered tissue substitutes. Organ building block (OBB)-based bioprinting has emerged as a promising strategy, utilizing microtissues with defined microarchitectural features as modular building units for three-dimensional bioprinting. This bottom-up approach facilitates the fabrication of personalized grafts that closely mimic the structural and functional characteristics of native tissues. In this review, we comprehensively summarize the current advances in OBB-based bioprinting technologies and their applications in reconstructive surgery, with a particular emphasis on cartilage, bone, vessels, muscle, and skin tissue reconstruction. We discuss the translational potential of this strategy, highlight key technical challenges, and propose future directions to facilitate clinical adoption. With ongoing innovation, OBB-based bioprinting holds the potential to revolutionize reconstructive surgery by enabling the production of functional, patient-specific tissue substitutes.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Core-shell microbead-based 3D vascularized glioma tumor model for effective drug testing.","authors":"Xiuxiu Zhang, Zixian Wang, Zeyang Liu, Zhen Zhan, Jianwei Chen, Tao Xu","doi":"10.1088/1758-5090/adebb5","DOIUrl":"10.1088/1758-5090/adebb5","url":null,"abstract":"<p><p>The 3D hydrogel-based tumor model demonstrates significant potential in replicating the physiological characteristics of<i>in vivo</i>tumor environments for mechanistic studies and drug testing. However, the challenge persists in accurately mimicking a vascularized microtumor with a compartmentalized structure in a controlled, heterogeneous, and high-throughput manner. This study introduces a vascularized 3D tumor model that incorporates an endothelial cell (EC) barrier, created by encapsulating glioma cells and human umbilical vein endothelial cells (HUVECs) within the core (6% gelatin) and shell (10% GelMa) of core-shell microbeads, respectively. Upon culture, the tumor cells develop spheroids within the liquid core, while the HUVECs in the shell migrate and adhere to the GelMa surface, ultimately forming an EC barrier. This 3D microengineered tumor model exhibits angiogenesis in solid tumor spheroids, effectively mirroring the<i>in vivo</i>structure and providing relevant biochemical and biophysical properties. Notably, in comparison to 2D cell cultures, the vascularized tumor model shows significantly higher half-maximal inhibitory concentrations for the anticancer drug doxorubicin. Collectively, these findings highlight the considerable potential of engineered 3D tumor models in drug testing.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bubble casting strategy to construct multifurcated hydrogel microtubes with adjustable dimensions and endothelialization.","authors":"Haonan Sun, Kunming Xing, Kexin Liu, Yumin Liu, Yuyan Li, Yingnan Sun, Shusheng Zhang","doi":"10.1088/1758-5090/adebb2","DOIUrl":"10.1088/1758-5090/adebb2","url":null,"abstract":"<p><p>The reconstruction of human tubular structures-characterized by adjustable small diameters (<6 mm), multifurcated morphologies, and biomimetic functionality-remains a significant challenge, particularly for researchers lacking specialized fabrication skills. In this work, we present a simple and effective strategy to fabricate freestanding, multifurcated hydrogel microtubes with tunable diameters, perfusability, and endothelialization capability by integrating stimuli-responsive hydrogels with a bubble casting technique. Leveraging the adhesive interaction between hydrogels and silicone molds, this method enables the formation of multifurcated hydrogel microtubes with uniform thickness and interconnected structures within modularly assembled molds. The integration of temperature-sensitive gelatine and photo-crosslinkable methacrylated gelatin (GelMA) permits the rapid and irreversible formation of robust hydrogel microtubes. A wide range of 2D structures including straight, L-shaped, T-shaped, bifurcated, and trifurcated microtubes can be readily produced, and further assembled into interconnected 3D microtube network using Lego-like assembly with the assistance of T- or Y-shaped adhesive connectors. The experimental results prove that the fabricated microtubes exhibit favorable physiological stability, mechanical strength, semi-permeability, hemocompatibility, cytocompatibility and anti-thrombogenicity. Moreover, the successful perfusion of whole rabbit blood and endothelialization with human umbilical vein endothelial cells (HUVECs) demonstrate their functional potential as biomimetic vascular scaffolds. Overall, our work introduces a robust, accessible, and modular strategy for generating multifurcated hydrogel microtubes featuring adjustable fine diameters. The technique is particularly suited for applications in tissue engineering and vascular modeling, and can be easily adopted by researchers across disciplines without the need for specialized equipment or training.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiofabricationPub Date : 2025-07-15DOI: 10.1088/1758-5090/ade933
Miranda Poklar, Ravikumar K, Connor Wiegand, Ben Mizerak, Ruiqi Wang, Rodrigo M Florentino, Zhenghao Liu, Alejandro Soto-Gutierrez, Prashant N Kumta, Ipsita Banerjee
{"title":"Bioprinting of human primary and iPSC-derived islets with retained and comparable functionality.","authors":"Miranda Poklar, Ravikumar K, Connor Wiegand, Ben Mizerak, Ruiqi Wang, Rodrigo M Florentino, Zhenghao Liu, Alejandro Soto-Gutierrez, Prashant N Kumta, Ipsita Banerjee","doi":"10.1088/1758-5090/ade933","DOIUrl":"https://doi.org/10.1088/1758-5090/ade933","url":null,"abstract":"<p><p>Currently, type 1 diabetes (T1D) can be treated through implantation of allogenic islets, which replenish the beta cell population, however this method requires an extensive post-implantation immunosuppressant regimen. Personalized cellular therapy can address this through implantation of an autologous cell population, induced pluripotent stem cells (iPSCs). Cellular therapy, however, requires an encapsulation device for implantation, and so to achieve this uniformly with cells in a clinical setting, bioprinting is a useful option. Bioprinting is dependent on having a bioink that is printable, retains structural fidelity after printing, and is supportive of cell type and function. While bioprinting of pancreatic islets has been demonstrated previously, success in maintaining islet function post-printing has been varied. The objective of this study is to investigate the feasibility of printing functional islets by determining the appropriate combination of bioink, printing parameters, and cell configuration. Here, we detail the successful bioprinting of both primary human islets and iPSC-derived islets embedded in an alginate/methylcellulose bioink, with functionality sustained within the construct for both cell lineages. Sc-RNAseq analysis also revealed that printing did not adversely affect the genetic expression and metabolic functionality of the iPSC-derived islets. Importantly, the iPSC-derived islets displayed comparable functionality to the primary islets, indicating the potential to act as a cell source alternative for T1D implantation.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":"17 3","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiofabricationPub Date : 2025-07-14DOI: 10.1088/1758-5090/add8bf
Kyung Seob Kim, Seung-Cheol Choi, Ji-Min Noh, Myeong-Hwa Song, Seongmin Jun, Ji Eun Na, Im Joo Rhyu, Do-Sun Lim
{"title":"Validation of the exosomal protein SERPINA11 as a potential atherosclerosis marker via bioprinted scaffold.","authors":"Kyung Seob Kim, Seung-Cheol Choi, Ji-Min Noh, Myeong-Hwa Song, Seongmin Jun, Ji Eun Na, Im Joo Rhyu, Do-Sun Lim","doi":"10.1088/1758-5090/add8bf","DOIUrl":"10.1088/1758-5090/add8bf","url":null,"abstract":"<p><p>Existing animal and human cell models have limitations in terms of heterogeneous differences or difficulties in sufficiently reproducing arterial structures and complex cell-cell interactions. The discovery of exosome-derived biomarkers using a three-dimensional (3D) bioprinted atherosclerosis model provides a noninvasive and stable detection method and is expected to contribute to the development of early diagnosis and personalized treatment. To contribute to the discovery of exosome-derived biomarkers related to the early diagnosis and prognosis of cardiovascular diseases using a 3D bioprinted atherosclerosis model, we reproduced an arterial environment using 3D bioprinting composed of a biocompatible extracellular matrix (bioink) and various human cells<i>in vitro</i>. The 3D bioprinted atherosclerosis model composed of inflammatory macrophages, coronary artery smooth muscle cells, coronary artery endothelial cells, and collagen methacryloyl (ColMA) hydrogel was treated with low-density lipoproteins to induce atherosclerosis, and the atherosclerosis model was classified into Baseline, early atherosclerosis (EA; Early Athero), and late atherosclerosis (LA; Late Athero) groups. The secreted exosomes were isolated according to the time period, and a characterization analysis was conducted to confirm the purity of the isolated exosomes. We evaluated the isolated exosomes qualitatively and quantitatively. Isolated exosomes were analyzed using proteomics and microRNA (miRNA) sequencing to verify whether the bioprinted atherosclerosis model induced atherosclerosis, and a novel EA biomarker, SERPINA11, was discovered. In conclusion, we verified that the bioprinted atherosclerosis model induced atherosclerosis and that the novel biomarker set of exosomal miRNAs (hsa-miR-143-5p and hsa-miR-6879-5p) expressed in EA and proteins (SERPINA11, AHSG, and F2) might be clinically useful in early diagnosis and prognosis.</p>","PeriodicalId":8964,"journal":{"name":"Biofabrication","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}