Biotechnology Letters最新文献

Comparative proteomic analysis to annotate the structural association of the hypothetical proteins from the conserved domain of P. aeruginosa as novel vaccine candidates.

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-19 DOI: 10.1007/s10529-024-03546-4

Prajval Tenginakai, Samiksha Bhor, Fathimathuz Zehra Waasia, Sameer Sharma, Susha Dinesh

{"title":"Comparative proteomic analysis to annotate the structural association of the hypothetical proteins from the conserved domain of P. aeruginosa as novel vaccine candidates.","authors":"Prajval Tenginakai, Samiksha Bhor, Fathimathuz Zehra Waasia, Sameer Sharma, Susha Dinesh","doi":"10.1007/s10529-024-03546-4","DOIUrl":"https://doi.org/10.1007/s10529-024-03546-4","url":null,"abstract":"Objectives: Pseudomonas aeruginosa, identified as an ESKAPE pathogen, contributes to severe clinical diseases worldwide and despite its prevalence an effective vaccine or treatment remains elusive. Numerous computational methods are being employed to target hypothetical proteins (HPs). Presently, no studies have predicted multi-epitope vaccines for these HPs.Results: Totally, 877 HPs from P. aeruginosa were included in the study and the data showcased here illustrate a methodical approach to prioritize the proteome by employing diverse comparative proteomics. The study employed physicochemical property assessment and conserved domain analysis to identify stable and immunologically pertinent proteins for epitope prediction. The VaxiJen2.0 antigenicity assessment aided in epitope selection, contributing to the foundational steps in vaccine development by predicting T-cell and B-cell epitopes. Potential T and B cell epitopes with high antigenicity, non-toxic categorization, and robust binding affinities were identified in the investigation. The periplasmic HP WP_132813935.1 was predicted as conserved, stable, and soluble. The T-cell peptide RTSMRALAY and the B-cell peptide MPVYLYLM were predicted to be probable non-allergen and demonstrated strong binding with MHC class I allele HLA-C*03:03.Conclusions: This research provides a comprehensive approach to predict T and B cell epitopes for conditions associated with P. aeruginosa, offering a candidate pool for tailored vaccine development. However, the efficacy of these epitopes in vaccine development necessitates clinical validation and testing for confirmation.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 1","pages":"13"},"PeriodicalIF":2.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocontrol toxicity of Trichoderma harzianum (Hypocreales: Hypocreaceae) derived chemical molecules against malarial mosquito Anopheles stephensi with molecular docking studies.

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-13 DOI: 10.1007/s10529-024-03542-8

Perumal Vivekanandhan, Abdullah A Alarfaj, Saleh Alfarraj, Mohammad Javed Ansari, Chinnaperumal Kamaraj

{"title":"Biocontrol toxicity of Trichoderma harzianum (Hypocreales: Hypocreaceae) derived chemical molecules against malarial mosquito Anopheles stephensi with molecular docking studies.","authors":"Perumal Vivekanandhan, Abdullah A Alarfaj, Saleh Alfarraj, Mohammad Javed Ansari, Chinnaperumal Kamaraj","doi":"10.1007/s10529-024-03542-8","DOIUrl":"https://doi.org/10.1007/s10529-024-03542-8","url":null,"abstract":"In this study, the crude chemical constituents extracted from Trichoderma harzianum and their toxicity were evaluated against the larvae, pupae, and adults of Anopheles stephensi at 24 and 48 h post-treatment. Additionally, the chemical constituents of the crude extracts were identified using gas chromatography-mass spectrometry (GC-MS) analysis, and their ability to bind with target proteins was confirmed through molecular docking studies. The results clearly demonstrated that the chemical compounds from T. harzianum exhibited promising mortality rates in larvae (98.66%), pupae (92%), and adult mosquitoes (81.33%) of A. stephensi 48 h after treatment. The study assessed the impact of crude extracts on insect enzymes 24 h post treatment, revealing significant alterations: a reduction in catalase activity and an increase in glutathione S-transferase levels compared to the control group. The treatment with crude chemical extracts resulted in mortality rates of 37.33% and 52% at 24 and 48 h, respectively, on Artemia salina , indicating minimal effects. After 48 h, the crude extract exhibited minimal toxicity on Eudrilus eugeniae, with a recorded mortality rate of 15% after 48 h. GC-MS analysis of T. harzianum-derived crude extracts identified ten major chemical constituents. Among these, chemicals, 2,4-bis(1,1-dimethylethyl) phenol (19.02%) was recognized as the predominant chemical component. This 2,4-bis(1,1-dimethylethyl) phenol molecule demonstrates a high binding affinity with target proteins, which is a key factor contributing to its insecticidal activity. This study concludes that the chemical constituents derived from T. harzianum are promising candidates for an eco-friendly, effective, and target-specific alternative control method for A. stephensi mosquitos.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 1","pages":"12"},"PeriodicalIF":2.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A recombinant L-threonine aldolase with high catalytic efficiency for the asymmetric synthesis of L-threo-phenylserine and L-threo-4-fluorophenylserine.

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-10 DOI: 10.1007/s10529-024-03553-5

Bijing Lei, Wan Jiang, Jinsong Ma, Caiyun Wang, Yinping Pan, Zhi Zhang, Bochu Wang, Jian Guo, Na Qi

{"title":"A recombinant L-threonine aldolase with high catalytic efficiency for the asymmetric synthesis of L-threo-phenylserine and L-threo-4-fluorophenylserine.","authors":"Bijing Lei, Wan Jiang, Jinsong Ma, Caiyun Wang, Yinping Pan, Zhi Zhang, Bochu Wang, Jian Guo, Na Qi","doi":"10.1007/s10529-024-03553-5","DOIUrl":"https://doi.org/10.1007/s10529-024-03553-5","url":null,"abstract":"Objectives: To develop robust variants of L-threonine aldolases (L-TAs), potent catalysts for synthesizing asymmetric β-hydroxy-α-amino acids, it is necessary to identify critical residues beyond the known active site residues.Results: Through virtual screening, a neglected residue Asn305, was identified as critical for catalytic efficiency. Subsequent site-saturation mutagenesis led to a potent variant N305R which exhibited excellent conversions of 88%conv (87%de) and 80%conv (94%de) for the synthesis of L-threo-phenylserine and L-threo-4-fluorophenylserine respectively. This variant not only outperformed the template enzyme, but also represented a promising L-TA for synthesizing the two β-hydroxy-α-amino acids. It was suggested that Arg305 of the variant N305R generated strong cation-arene interaction and electrostatic force with the intermediates, leading to strengthened binding, enhanced L-threo favored orientation and wider entrance.Conclusions: Our work not only provided an excellent variant N305R, but also suggested the crucial function of a neglected residue Asn305, which offered valuable experiences for other L-TA studies.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 1","pages":"11"},"PeriodicalIF":2.0,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification, heterologous expression, and characterisation of β-1,3-xylanase BcXyn26B from human gut bacterium Bacteroides cellulosilyticus WH2.

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-02 DOI: 10.1007/s10529-024-03547-3

Sanae Hori, Fumiyoshi Okazaki

{"title":"Identification, heterologous expression, and characterisation of β-1,3-xylanase BcXyn26B from human gut bacterium Bacteroides cellulosilyticus WH2.","authors":"Sanae Hori, Fumiyoshi Okazaki","doi":"10.1007/s10529-024-03547-3","DOIUrl":"10.1007/s10529-024-03547-3","url":null,"abstract":"The cell walls of red and green algae contain β-1,3-xylan, which is hydrolysed by the endo-type enzyme β-1,3-xylanase. Notably, only marine-bacteria-derived β-1,3-xylanases have been functionally characterised to date. In this study, we characterised the enzymatic properties of a potential β-1,3-xylanase (BcXyn26B) derived from the human gut bacterium, Bacteroides cellulosilyticus WH2. The codon optimized BcXyn26B gene was synthesised and expressed in Escherichia coli BL21(DE3). The recombinant protein was purified by a two-step purification process using Ni-affinity chromatography followed by anion exchange chromatography, and its enzymatic properties were characterised. The recombinant BcXyn26B exhibited specific hydrolytic activity against β-1,3-xylan and released various β-1,3-xylooligosaccharides, with β-1,3-xylobiose as the primary product. The optimum reaction temperature was 50 °C, higher than that for other enzymes derived from marine bacteria. This study represents the first report on the identification, heterologous expression, and characterisation of β-1,3-xylanase from human gut microbes. Notably, the substrate specificity of BcXyn26B indicates that human gut Bacteroides species possess an unknown β-1,3-xylan utilisation system.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 1","pages":"10"},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11611983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Homologous expression of Aspergillus niger α-L-rhamnosidase and its application in enzymatic debittering of Ougan juice. 黑曲霉α-L-鼠李糖酶的同源表达及其在乌干果汁酶解脱盐中的应用。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-09-05 DOI: 10.1007/s10529-024-03531-x

Fei Zhang, Xue Wang, Lixia Pan, Zhao Wang, Jianyong Zheng

{"title":"Homologous expression of Aspergillus niger α-L-rhamnosidase and its application in enzymatic debittering of Ougan juice.","authors":"Fei Zhang, Xue Wang, Lixia Pan, Zhao Wang, Jianyong Zheng","doi":"10.1007/s10529-024-03531-x","DOIUrl":"10.1007/s10529-024-03531-x","url":null,"abstract":"The α-L-rhamnosidase (rha1) gene was homologously expressed in Aspergillus niger strains CCTCC 206047 and CCTCC 206047ΔpyrG, using hygromycin B and auxotrophic as selection markers. The engineered A. niger strains RHA001-1 and RHA003-1 were screened, yielding α-L-rhamnosidase activities of 20.81 ± 0.56 U/mL and 15.35 ± 0.87 U/mL, respectively. The copy numbers of the rha1 gene in strains RHA001-1 and RHA003-1 were found to be 18 and 14, respectively. Correlation analysis between copy number and enzyme activity in the A. niger strains revealed that α-L-rhamnosidase activity increased with the copy number of the rha1 gene. Recombinant α-L-rhamnosidase was utilized for the enzymatic debittering of Ougan juice, and its process conditions were optimized. Furthermore, the primary bitter substance neohesperidin (2.22 g/L) in Ougan juice was converted into hesperetin 7-O-glucoside (1.47 g/L) and hesperidin (0.143 g/L). This study presents a novel approach for the production of food-grade α-L-rhamnosidase and establishes a technical foundation for its application in the beverage industry.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"1187-1198"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142131748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic diversity assessment of Palestinian safflower (Carthamus tinctorius L.) utilizing DAMD molecular markers. 利用 DAMD 分子标记评估巴勒斯坦红花（Carthamus tinctorius L.）的遗传多样性。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-10-19 DOI: 10.1007/s10529-024-03538-4

Yamen A S Hamdan

{"title":"Genetic diversity assessment of Palestinian safflower (Carthamus tinctorius L.) utilizing DAMD molecular markers.","authors":"Yamen A S Hamdan","doi":"10.1007/s10529-024-03538-4","DOIUrl":"10.1007/s10529-024-03538-4","url":null,"abstract":"The current knowledge about Palestinian safflower landraces is relatively limited in terms of phenotypic and molecular characterization, however, the purpose of this investigation was to determine the amount of genetic diversity in eighteen local safflower landraces using seven DAMD markers. The banding patterns for each primer were scored and compiled into a data matrix. Subsequently, the data matrix was analyzed using UPGMA cluster analysis to identify distinct genetic groups among the landraces. In total, 88 DNA fragments were found, and there were an average of 12.6 loci per assay unit observed. Resolving Power (RP) revealed an average of 7.09 was determined, with the highest RP value at 13.3. The dendrogram obtained from DAMD data divided the landraces into three main clusters, denoted as I, II and III. The first cluster (I) consisted of one genotype (PTUK.SA16). The second cluster (II) consisted of two genotypes (PTUK.SA13 and PTUK.SA10). The third cluster (III) was later partitioned into two distinct sub-clusters, which are III.a and III.b. Sub-cluster III.a comprised seven genotypes (PTUK.SA4, PTUK.SA9, PTUK.SA8, PTUK.SA7, PTUK.SA6, PTUK.SA5 and PTUK.SA3). While Sub-cluster III.b consisted of eight genotypes (PTUK.SA15, PTUK.SA18, PTUK.SA17, PTUK.SA14, PTUK.SA12, PTUK.SA2, PTUK.SA11, and PTUK.SA1). This research assess the genetic diversity of Palestinian safflower landraces using PCR-based DAMD markers. The remarkable level of polymorphism detected using DAMD markers demonstrated their effectiveness in distinguishing between Palestinian safflower genotypes.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"1293-1302"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered probiotic E.coli Nissle 1917 for release PTEN to improve the tumor microenvironment and suppress tumor growth. 设计益生菌大肠杆菌 Nissle 1917 以释放 PTEN，从而改善肿瘤微环境并抑制肿瘤生长。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-09-27 DOI: 10.1007/s10529-024-03536-6

Zirui Dai, Wenjuan Zhao, Li Cao, Zirong Zhu, Ziyuan Xia, Liqiu Xia

{"title":"Engineered probiotic E.coli Nissle 1917 for release PTEN to improve the tumor microenvironment and suppress tumor growth.","authors":"Zirui Dai, Wenjuan Zhao, Li Cao, Zirong Zhu, Ziyuan Xia, Liqiu Xia","doi":"10.1007/s10529-024-03536-6","DOIUrl":"10.1007/s10529-024-03536-6","url":null,"abstract":"The cancer is one of the diseases of serious threat to people's health and life nowadays. But heterogeneity, drug resistance and treatment side effects of cancer, traditional treatments still have limitations. Tumor-targeting probiotics with a well-established Biosafety and efficient targeting as a delivery vectors to deliver anticancer genes or antitumor drugs to tumor microenvironment has attracted much attention in cancer therapies. In this study, E.coil Nissle 1917 (EcN) was utilized to deliver eukaryotic anti-tumor protein PTEN to tumor microenvironment and suppress tumor growth. Therefore, the EcN (PTEN) was developed. Our results demonstrated that EcN (PTEN) could colonize the tumor site accurately and inhibit the growth of colorectal cancer cells in tumor-bearing mice. It is worth noting that the tumor microenvironment of the treated mice showed significant recruitment of and M1 macrophages, neutrophils and T lymphocytes. No toxicity was observed in the normal tissues during the experiments. This research show the probiotic EcN(PTEN) holds the promise of becoming a powerful weapon against cancer and expected to provide more effective treatments for cancer patients.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"1237-1247"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research progress in the biosynthesis of xylitol: feedstock evolution from xylose to glucose. 木糖醇生物合成的研究进展：从木糖到葡萄糖的原料演变。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-09-28 DOI: 10.1007/s10529-024-03535-7

Xin-Yu Zhang, Xi-Min Zhao, Xin-Yu Shi, Ying-Jie Mei, Xiao-Jie Ren, Xin-He Zhao

引用次数: 0

Toxicants improve glycerol production in the fermentation of undetoxified hydrolysate by Candida glycerinogenes. 在甘油酸根念珠菌发酵未解毒水解物的过程中，毒物可提高甘油产量。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-07-31 DOI: 10.1007/s10529-024-03503-1

Xiaohong Zhao, Hong Zong, Xinyao Lu, Bin Zhuge

{"title":"Toxicants improve glycerol production in the fermentation of undetoxified hydrolysate by Candida glycerinogenes.","authors":"Xiaohong Zhao, Hong Zong, Xinyao Lu, Bin Zhuge","doi":"10.1007/s10529-024-03503-1","DOIUrl":"10.1007/s10529-024-03503-1","url":null,"abstract":"Objectives: Toxicants inhibit microbial fermentation and reduce product titres. This work investigated the glycerol production characteristics of Candida glycerinogenes in highly toxic unwashed undetoxified hydrolysate and provided new ideas for high glycerol production from hydrolysates.Results: The unwashed hydrolysate contains higher concentrations of toxicants, such as furfural, acetic acid, phenols and NaCl than the washed alkali-treated bagasse hydrolysate. C. glycerinogenes fermented unwashed undetoxified hydrolysate yielded 36.1 g/L glycerol, 15.8% higher than the washed hydrolysate, suggesting that the toxicants stimulated glycerol synthesis. qRT-PCR analysis showed that toxicants of unwashed undetoxified hydrolysates greatly up-regulated the transcript levels of the genes GPD1, HXT4 and MSN4 et al. Overexpressing the above genes increased glycerol production by 27.9% to 46.1 g/L. And it was further increased by 8.8% to 50.1 g/L in a 5 L bioreactor.Conclusions: This result proves that toxicants in lignocellulosic hydrolysates can increase the titre of microbial glycerol production.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"1057-1068"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141858926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lentilactobacillus farraginis FSI (3): a whole cell biocatalyst for the synthesis of kojic acid derivative under aquatic condition. 远拉氏扁豆乳杆菌 FSI (3)：水生条件下合成曲酸衍生物的全细胞生物催化剂。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-08-20 DOI: 10.1007/s10529-024-03514-y

Mangal A Chaudhari, Pratiksha R Wankhede, Kiran S Dalal, Arun D Kale, Dipak S Dalal, Bhushan L Chaudhari

{"title":"Lentilactobacillus farraginis FSI (3): a whole cell biocatalyst for the synthesis of kojic acid derivative under aquatic condition.","authors":"Mangal A Chaudhari, Pratiksha R Wankhede, Kiran S Dalal, Arun D Kale, Dipak S Dalal, Bhushan L Chaudhari","doi":"10.1007/s10529-024-03514-y","DOIUrl":"10.1007/s10529-024-03514-y","url":null,"abstract":"Kojic acid derivatives are useful in the cosmetics and pharmaceutical industries. The current investigation focuses on the search for a safe and environmentally friendly newer whole-cell biocatalyst for the synthesis of kojic acid derivative especially 2-amino-6-(hydroxymethyl)-8-oxo-4-phenyl-4,8-dihydropyrano[3,2-b]pyran-3-carbonitrile (APhCN). In this context, a total of six cultures were isolated from fecal samples of infants and subjected to probiotic characterization followed by screening as whole cell biocatalyst (WCB). In this multicomponent reaction, benzaldehyde, malononitrile, and kojic acid were used to synthesize APhCN at room temperature under aqueous conditions. The screening of potent whole cell biocatalyst (WCB) from isolated cultures was done by comparing reaction time and percent yield. The potent WCB gave a good yield of 95% within 15 h of time and hence further characterized biochemically and identified as Lentilactobacillus farraginis by using 16S rRNA gene sequencing. Lactobacilli having GRAS (generally regarded as safe) status and being able to carry out this transformation under moderate reaction conditions with easy recovery of both product and biocatalyst, it has the potential to replace some of the chemical catalytic methods.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"1107-1120"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142003511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0