Biotechnology Letters最新文献

Homologous expression of Aspergillus niger α-L-rhamnosidase and its application in enzymatic debittering of Ougan juice. 黑曲霉α-L-鼠李糖酶的同源表达及其在乌干果汁酶解脱盐中的应用。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-09-05 DOI: 10.1007/s10529-024-03531-x

Fei Zhang, Xue Wang, Lixia Pan, Zhao Wang, Jianyong Zheng

{"title":"Homologous expression of Aspergillus niger α-L-rhamnosidase and its application in enzymatic debittering of Ougan juice.","authors":"Fei Zhang, Xue Wang, Lixia Pan, Zhao Wang, Jianyong Zheng","doi":"10.1007/s10529-024-03531-x","DOIUrl":"10.1007/s10529-024-03531-x","url":null,"abstract":"The α-L-rhamnosidase (rha1) gene was homologously expressed in Aspergillus niger strains CCTCC 206047 and CCTCC 206047ΔpyrG, using hygromycin B and auxotrophic as selection markers. The engineered A. niger strains RHA001-1 and RHA003-1 were screened, yielding α-L-rhamnosidase activities of 20.81 ± 0.56 U/mL and 15.35 ± 0.87 U/mL, respectively. The copy numbers of the rha1 gene in strains RHA001-1 and RHA003-1 were found to be 18 and 14, respectively. Correlation analysis between copy number and enzyme activity in the A. niger strains revealed that α-L-rhamnosidase activity increased with the copy number of the rha1 gene. Recombinant α-L-rhamnosidase was utilized for the enzymatic debittering of Ougan juice, and its process conditions were optimized. Furthermore, the primary bitter substance neohesperidin (2.22 g/L) in Ougan juice was converted into hesperetin 7-O-glucoside (1.47 g/L) and hesperidin (0.143 g/L). This study presents a novel approach for the production of food-grade α-L-rhamnosidase and establishes a technical foundation for its application in the beverage industry.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"1187-1198"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142131748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic diversity assessment of Palestinian safflower (Carthamus tinctorius L.) utilizing DAMD molecular markers. 利用 DAMD 分子标记评估巴勒斯坦红花（Carthamus tinctorius L.）的遗传多样性。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-10-19 DOI: 10.1007/s10529-024-03538-4

Yamen A S Hamdan

{"title":"Genetic diversity assessment of Palestinian safflower (Carthamus tinctorius L.) utilizing DAMD molecular markers.","authors":"Yamen A S Hamdan","doi":"10.1007/s10529-024-03538-4","DOIUrl":"10.1007/s10529-024-03538-4","url":null,"abstract":"The current knowledge about Palestinian safflower landraces is relatively limited in terms of phenotypic and molecular characterization, however, the purpose of this investigation was to determine the amount of genetic diversity in eighteen local safflower landraces using seven DAMD markers. The banding patterns for each primer were scored and compiled into a data matrix. Subsequently, the data matrix was analyzed using UPGMA cluster analysis to identify distinct genetic groups among the landraces. In total, 88 DNA fragments were found, and there were an average of 12.6 loci per assay unit observed. Resolving Power (RP) revealed an average of 7.09 was determined, with the highest RP value at 13.3. The dendrogram obtained from DAMD data divided the landraces into three main clusters, denoted as I, II and III. The first cluster (I) consisted of one genotype (PTUK.SA16). The second cluster (II) consisted of two genotypes (PTUK.SA13 and PTUK.SA10). The third cluster (III) was later partitioned into two distinct sub-clusters, which are III.a and III.b. Sub-cluster III.a comprised seven genotypes (PTUK.SA4, PTUK.SA9, PTUK.SA8, PTUK.SA7, PTUK.SA6, PTUK.SA5 and PTUK.SA3). While Sub-cluster III.b consisted of eight genotypes (PTUK.SA15, PTUK.SA18, PTUK.SA17, PTUK.SA14, PTUK.SA12, PTUK.SA2, PTUK.SA11, and PTUK.SA1). This research assess the genetic diversity of Palestinian safflower landraces using PCR-based DAMD markers. The remarkable level of polymorphism detected using DAMD markers demonstrated their effectiveness in distinguishing between Palestinian safflower genotypes.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"1293-1302"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered probiotic E.coli Nissle 1917 for release PTEN to improve the tumor microenvironment and suppress tumor growth. 设计益生菌大肠杆菌 Nissle 1917 以释放 PTEN，从而改善肿瘤微环境并抑制肿瘤生长。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-09-27 DOI: 10.1007/s10529-024-03536-6

Zirui Dai, Wenjuan Zhao, Li Cao, Zirong Zhu, Ziyuan Xia, Liqiu Xia

{"title":"Engineered probiotic E.coli Nissle 1917 for release PTEN to improve the tumor microenvironment and suppress tumor growth.","authors":"Zirui Dai, Wenjuan Zhao, Li Cao, Zirong Zhu, Ziyuan Xia, Liqiu Xia","doi":"10.1007/s10529-024-03536-6","DOIUrl":"10.1007/s10529-024-03536-6","url":null,"abstract":"The cancer is one of the diseases of serious threat to people's health and life nowadays. But heterogeneity, drug resistance and treatment side effects of cancer, traditional treatments still have limitations. Tumor-targeting probiotics with a well-established Biosafety and efficient targeting as a delivery vectors to deliver anticancer genes or antitumor drugs to tumor microenvironment has attracted much attention in cancer therapies. In this study, E.coil Nissle 1917 (EcN) was utilized to deliver eukaryotic anti-tumor protein PTEN to tumor microenvironment and suppress tumor growth. Therefore, the EcN (PTEN) was developed. Our results demonstrated that EcN (PTEN) could colonize the tumor site accurately and inhibit the growth of colorectal cancer cells in tumor-bearing mice. It is worth noting that the tumor microenvironment of the treated mice showed significant recruitment of and M1 macrophages, neutrophils and T lymphocytes. No toxicity was observed in the normal tissues during the experiments. This research show the probiotic EcN(PTEN) holds the promise of becoming a powerful weapon against cancer and expected to provide more effective treatments for cancer patients.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"1237-1247"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research progress in the biosynthesis of xylitol: feedstock evolution from xylose to glucose. 木糖醇生物合成的研究进展：从木糖到葡萄糖的原料演变。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-09-28 DOI: 10.1007/s10529-024-03535-7

Xin-Yu Zhang, Xi-Min Zhao, Xin-Yu Shi, Ying-Jie Mei, Xiao-Jie Ren, Xin-He Zhao

引用次数: 0

Toxicants improve glycerol production in the fermentation of undetoxified hydrolysate by Candida glycerinogenes. 在甘油酸根念珠菌发酵未解毒水解物的过程中，毒物可提高甘油产量。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-07-31 DOI: 10.1007/s10529-024-03503-1

Xiaohong Zhao, Hong Zong, Xinyao Lu, Bin Zhuge

{"title":"Toxicants improve glycerol production in the fermentation of undetoxified hydrolysate by Candida glycerinogenes.","authors":"Xiaohong Zhao, Hong Zong, Xinyao Lu, Bin Zhuge","doi":"10.1007/s10529-024-03503-1","DOIUrl":"10.1007/s10529-024-03503-1","url":null,"abstract":"Objectives: Toxicants inhibit microbial fermentation and reduce product titres. This work investigated the glycerol production characteristics of Candida glycerinogenes in highly toxic unwashed undetoxified hydrolysate and provided new ideas for high glycerol production from hydrolysates.Results: The unwashed hydrolysate contains higher concentrations of toxicants, such as furfural, acetic acid, phenols and NaCl than the washed alkali-treated bagasse hydrolysate. C. glycerinogenes fermented unwashed undetoxified hydrolysate yielded 36.1 g/L glycerol, 15.8% higher than the washed hydrolysate, suggesting that the toxicants stimulated glycerol synthesis. qRT-PCR analysis showed that toxicants of unwashed undetoxified hydrolysates greatly up-regulated the transcript levels of the genes GPD1, HXT4 and MSN4 et al. Overexpressing the above genes increased glycerol production by 27.9% to 46.1 g/L. And it was further increased by 8.8% to 50.1 g/L in a 5 L bioreactor.Conclusions: This result proves that toxicants in lignocellulosic hydrolysates can increase the titre of microbial glycerol production.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"1057-1068"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141858926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lentilactobacillus farraginis FSI (3): a whole cell biocatalyst for the synthesis of kojic acid derivative under aquatic condition. 远拉氏扁豆乳杆菌 FSI (3)：水生条件下合成曲酸衍生物的全细胞生物催化剂。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-08-20 DOI: 10.1007/s10529-024-03514-y

Mangal A Chaudhari, Pratiksha R Wankhede, Kiran S Dalal, Arun D Kale, Dipak S Dalal, Bhushan L Chaudhari

{"title":"Lentilactobacillus farraginis FSI (3): a whole cell biocatalyst for the synthesis of kojic acid derivative under aquatic condition.","authors":"Mangal A Chaudhari, Pratiksha R Wankhede, Kiran S Dalal, Arun D Kale, Dipak S Dalal, Bhushan L Chaudhari","doi":"10.1007/s10529-024-03514-y","DOIUrl":"10.1007/s10529-024-03514-y","url":null,"abstract":"Kojic acid derivatives are useful in the cosmetics and pharmaceutical industries. The current investigation focuses on the search for a safe and environmentally friendly newer whole-cell biocatalyst for the synthesis of kojic acid derivative especially 2-amino-6-(hydroxymethyl)-8-oxo-4-phenyl-4,8-dihydropyrano[3,2-b]pyran-3-carbonitrile (APhCN). In this context, a total of six cultures were isolated from fecal samples of infants and subjected to probiotic characterization followed by screening as whole cell biocatalyst (WCB). In this multicomponent reaction, benzaldehyde, malononitrile, and kojic acid were used to synthesize APhCN at room temperature under aqueous conditions. The screening of potent whole cell biocatalyst (WCB) from isolated cultures was done by comparing reaction time and percent yield. The potent WCB gave a good yield of 95% within 15 h of time and hence further characterized biochemically and identified as Lentilactobacillus farraginis by using 16S rRNA gene sequencing. Lactobacilli having GRAS (generally regarded as safe) status and being able to carry out this transformation under moderate reaction conditions with easy recovery of both product and biocatalyst, it has the potential to replace some of the chemical catalytic methods.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"1107-1120"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142003511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical characterization of a recombinant laccase from Halalkalibacterium halodurans C-125 and its application in the biotransformation of organic compounds. Halalkalibacterium halodurans C-125 重组漆酶的生化特征及其在有机化合物生物转化中的应用。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-10-28 DOI: 10.1007/s10529-024-03532-w

Jihene Maati, Jolanta Polak, Monika Janczarek, Marcin Grąz, Issam Smaali, Anna Jarosz-Wilkołazka

{"title":"Biochemical characterization of a recombinant laccase from Halalkalibacterium halodurans C-125 and its application in the biotransformation of organic compounds.","authors":"Jihene Maati, Jolanta Polak, Monika Janczarek, Marcin Grąz, Issam Smaali, Anna Jarosz-Wilkołazka","doi":"10.1007/s10529-024-03532-w","DOIUrl":"10.1007/s10529-024-03532-w","url":null,"abstract":"Objectives: This study aimed to produce an engineered recombinant laccase from extremophilic Halalkalibacterium halodurans C-125 (Lac-HhC-125) with higher protein yield, into a more active conformation and with properties that meet the fundamental needs of biotechnological application.Results: The rLac-HhC125 was partially purified by size exclusion chromatography and concentrated by ultrafiltration (10 kDa) with a yield of 57.6%. Oxidation reactions showed that adding 2 mM CuSO4 to the assay solution led to activating the laccase. To increase its initial activity, the rLac-HhC125 was treated at 50 °C for 20 min before the assays, improving its performance by fourfold using the syringaldazine as a substrate. When treated with EDTA, methanol, ethanol, and DMSO, the rLac-HhC125 maintained more than 80% of its original activity. Interestingly, the acetonitrile induced a twofold activity of the rLac-HhC125. The putative rLac-HhC125 demonstrated a capability of efficient transformation of different organic compounds at pH 6, known as dye precursors, into coloured molecules.Conclusion: The rLac-HhC125 was active at high temperatures and alkaline pH, exhibited tolerance to organic solvents, and efficiently transformed different hydroxy derivatives into coloured compounds, which indicates that it can be used in various biotechnological processes.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"1199-1218"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11550293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-yield magnetosome production of Magnetospirillum magneticum strain AMB-1 in flask fermentation through simplified processing and optimized iron supplementation. 通过简化处理和优化补铁，在烧瓶发酵过程中高产生产磁螺菌菌株 AMB-1 的磁小体。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-07-20 DOI: 10.1007/s10529-024-03507-x

Yu Wang, Zhengyi Liu, Wenjun Li, Hongli Cui, Yandi Huang, Song Qin

{"title":"High-yield magnetosome production of Magnetospirillum magneticum strain AMB-1 in flask fermentation through simplified processing and optimized iron supplementation.","authors":"Yu Wang, Zhengyi Liu, Wenjun Li, Hongli Cui, Yandi Huang, Song Qin","doi":"10.1007/s10529-024-03507-x","DOIUrl":"10.1007/s10529-024-03507-x","url":null,"abstract":"Objectives: Developing a simplified flask fermentation strategy utilizing magnetotactic bacterium AMB-1 and optimized iron supplementation for high-yield magnetosome production to address the challenges associated with magnetosome acquisition.Results: A reliable processing for the pure culture of AMB-1 was established using standard laboratory consumables and equipment. Subsequently, the medium and iron supplementation were optimized to enhance the yield of AMB-1 magnetosomes. The mSLM supported higher biomass accumulation in flask fermentation, reaching an OD565 of ~ 0.7. The premixed solution of ferric quinate and EDTA-Fe (at a ratio of 0.5:0.5 and a concentration of 0.4 mmol/L) stabilized Fe3+ and significantly increased the reductase activity of AMB-1. Flask fermentations with an initial volume of 15 L were then conducted employing the optimized fermentation strategy. After two rounds of iron and nutrient supplementation, the magnetosome yield reached 185.7 ± 9.5 mg/batch (approximately 12 mg/L), representing the highest AMB-1 flask fermentation yield to our knowledge.Conclusion: A flask fermentation strategy for high-yield magnetsome production was developed, eliminating the need for bioreactors and greatly simplifying the process of magnetosome acquisition.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"1069-1083"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141730993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A key component Rxt3 in the Rpd3L histone deacetylase complex regulates development, stress tolerance, amylase production and kojic acid synthesis in Aspergillus oryzae. Rpd3L 组蛋白去乙酰化酶复合物中的一个关键成分 Rxt3 调节黑曲霉的发育、抗逆性、淀粉酶的产生和曲酸的合成。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-07-31 DOI: 10.1007/s10529-024-03515-x

Chaofeng Chang, Herui Wang, Yiling Liu, Yiting Xie, Dingxiang Xue, Feng Zhang

引用次数: 0

Multilevel metabolic engineering for enhanced synthesis of S-adenosylmethionine by Bacillus amyloliquefaciens. 通过多级代谢工程提高淀粉样芽孢杆菌合成 S-腺苷蛋氨酸的能力。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-08-20 DOI: 10.1007/s10529-024-03523-x

Cong Jiang, Dian Zou, Liying Ruan, Wenyuan Han, Xuetuan Wei

引用次数: 0