Biotechnology Letters最新文献_第2页

Mesenchymal stem cell-derived cell-free technologies: a patent landscape. 间充质干细胞衍生的无细胞技术：专利概况。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-10-01 Epub Date: 2024-06-20 DOI: 10.1007/s10529-024-03506-y

Luiza Carolina França Opretzka, Cláudio Damasceno Pinto, Jânio Rodrigo de Jesus Santos, Alyne Almeida de Lima, Milena Botelho Pereira Soares, Cristiane Flora Villarreal

引用次数: 0

Nepeta cataria L. (catnip) can serve as a chassis for the engineering of secondary metabolic pathways. 猫薄荷（Nepeta cataria L.）可以作为次级代谢途径工程的底盘。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-10-01 Epub Date: 2024-05-08 DOI: 10.1007/s10529-024-03489-w

Marcus Geissler, Christoph Neubauer, Yuriy V Sheludko, Adrian Brückner, Heribert Warzecha

{"title":"Nepeta cataria L. (catnip) can serve as a chassis for the engineering of secondary metabolic pathways.","authors":"Marcus Geissler, Christoph Neubauer, Yuriy V Sheludko, Adrian Brückner, Heribert Warzecha","doi":"10.1007/s10529-024-03489-w","DOIUrl":"10.1007/s10529-024-03489-w","url":null,"abstract":"Objective: Evaluation of Nepeta cataria as a host with specific endogenous metabolite background for transient expression and metabolic engineering of secondary biosynthetic sequences.Results: The reporter gene gfp::licBM3 as well as three biosynthetic genes leading to the formation of the cannabinoid precursor olivetolic acid were adopted to the modular cloning standard GoldenBraid, transiently expressed in two chemotypes of N. cataria and compared to Nicotiana benthamiana. To estimate the expression efficiency in both hosts, quantification of the reporter activity was carried out with a sensitive and specific lichenase assay. While N. benthamiana exhibited lichenase activity of 676 ± 94 μmol g-1 s-1, N. cataria cultivar '1000', and the cultivar 'Citriodora' showed an activity of 37 ± 8 μmol g-1 s-1 and 18 ± 4 μmol g-1 s-1, respectively. Further, combinatorial expression of genes involved in cannabinoid biosynthetic pathway acyl-activating enzyme 1 (aae1), olivetol synthase (ols) and olivetolic acid cyclase (oac) in N. cataria cv. resulted presumably in the in vivo production of olivetolic acid glycosides.Conclusion: Nepeta cataria is amenable to Agrobacterium-mediated transient expression and could serve as a novel chassis for the engineering of secondary metabolic pathways and transient evaluation of heterologous genes.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140875752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of L-cysteine on starch and protein degradation during barley germination. L-半胱氨酸对大麦发芽过程中淀粉和蛋白质降解的影响

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-10-01 Epub Date: 2024-06-25 DOI: 10.1007/s10529-024-03508-w

Shumin Hu, Qingqing Qin, Cui Zhang, Junhong Yu, Shuli Huang, Jia Liu, Zhaoxia Yang

{"title":"The effect of L-cysteine on starch and protein degradation during barley germination.","authors":"Shumin Hu, Qingqing Qin, Cui Zhang, Junhong Yu, Shuli Huang, Jia Liu, Zhaoxia Yang","doi":"10.1007/s10529-024-03508-w","DOIUrl":"10.1007/s10529-024-03508-w","url":null,"abstract":"Objectives: In order to investigate the impact of L-cysteine (L-Cys) on starch and protein degradation during barley germination. The amylase activities, degradation of macromolecules during germination were determined in this study.Methods: Barley was germinated in petri dish for 0 to 5 days with different levels of L-Cys (0 mM, 2.5 mM, 5 mM, 10 mM).Results: L-Cys addition increased the total limit dextrinase (LD) activities and decreased the LD inhibitor activities during whole germination stage. The activities of α-amylase, β-amylase and free LD were increased with the addition of 2.5, 5 mM L-Cys at germination days 1 to 4. Due to higher amylase in malt with the addition of L-Cys, the non-fermentable sugars were reduced and the glucose, maltotriose were improved. Furthermore, the protein degradation analysis showed that low molecular weight protein increased and middle molecular weight protein decreased obviously in wort from the malt germinated with L-Cys, demonstrating that the L-Cys promote the protein degradation. Lastly, the filtration performance of malt with the addition of L-Cys during malting was better than the control.Conclusion: In conclusion, L-Cys can promote the degradation of storage material (starch, protein) during barley germination, leading to a better green malt quality.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141445355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrile hydratase as a promising biocatalyst: recent advances and future prospects 腈水解酶作为一种前景广阔的生物催化剂：最新进展与未来展望

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-09-13 DOI: 10.1007/s10529-024-03530-y

Chao Feng, Jing Chen, Wenxin Ye, Zhanshi Wang

{"title":"Nitrile hydratase as a promising biocatalyst: recent advances and future prospects","authors":"Chao Feng, Jing Chen, Wenxin Ye, Zhanshi Wang","doi":"10.1007/s10529-024-03530-y","DOIUrl":"https://doi.org/10.1007/s10529-024-03530-y","url":null,"abstract":"Amides are an important type of synthetic intermediate used in the chemical, agrochemical, pharmaceutical, and nutraceutical industries. The traditional chemical process of converting nitriles into the corresponding amides is feasible but is restricted because of the harsh conditions required. In recent decades, nitrile hydratase (NHase, EC 4.2.1.84) has attracted considerable attention because of its application in nitrile transformation as a prominent biocatalyst. In this review, we provide a comprehensive survey of recent advances in NHase research in terms of natural distribution, enzyme screening, and molecular modification on the basis of its characteristics and catalytic mechanism. Additionally, industrial applications and recent significant biotechnology advances in NHase bioengineering and immobilization techniques are systematically summarized. Moreover, the current challenges and future perspectives for its further development in industrial applications for green chemistry were also discussed. This study contributes to the current state-of-the-art, providing important technical information for new NHase applications in manufacturing industries.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immobilization coupling with aptamer assisted dual cycle amplification for sensitive sEVs isolation and analysis 用于灵敏 sEVs 分离和分析的固定耦合与适配体辅助双循环扩增技术

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-09-12 DOI: 10.1007/s10529-024-03526-8

Yu He, Ying Ren, Jiawen Tang

{"title":"Immobilization coupling with aptamer assisted dual cycle amplification for sensitive sEVs isolation and analysis","authors":"Yu He, Ying Ren, Jiawen Tang","doi":"10.1007/s10529-024-03526-8","DOIUrl":"https://doi.org/10.1007/s10529-024-03526-8","url":null,"abstract":"Precise identification of small extracellular vesicles (sEVs) is crucial for improving disease diagnosis and treatments, such as bladder cancer. However, accurate isolation and simultaneously quantification of sEVs remain a huge challenge. We have introduced a new technique that combines immobilization with aptamer-assisted dual cycle amplification to isolate and analyze sEVs with high sensitivity. In this method, the CD9 protein antibody is attached to the plate’s surface for the initial identification of sEVs, while an aptamer probe is used to detect the exosomal surface protein CD63. We have created an sEVs-surface method that combines target recognition initiated signal recycling and rolling circle amplification (RCA) for signal amplification. This approach allows for the “AND” logic analysis of dual biomarkers, enabling both sEVs quantification and tracing. The proposed approach has a broad detection range and a low limit of detection. Moreover, the established method showed good stability in detecting sEVs with a low coefficient of variation. Our method can effectively isolate certain sEVs and accurately identify them, making it suitable for many uses in biological science, biomedical engineering, and personalized medicine.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors affecting rAAV titers during triple-plasmid transient transfection in HEK-293 cells 影响 HEK-293 细胞中三重质粒瞬时转染过程中 rAAV 滴度的因素

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-09-11 DOI: 10.1007/s10529-024-03520-0

Martina Pistek, Peter Andorfer, Reingard Grabherr, Barbara Kraus, Juan A. Hernandez Bort

{"title":"Factors affecting rAAV titers during triple-plasmid transient transfection in HEK-293 cells","authors":"Martina Pistek, Peter Andorfer, Reingard Grabherr, Barbara Kraus, Juan A. Hernandez Bort","doi":"10.1007/s10529-024-03520-0","DOIUrl":"https://doi.org/10.1007/s10529-024-03520-0","url":null,"abstract":"The efficiency of triple-plasmid transfection in recombinant Adeno-Associated Virus (rAAV) production was analyzed by examining two distinct HEK-293 cells lines. These were categorized as high producer (HP) and low producer (LP) based on their differing levels of productivity under identical conditions. Analysis of RNA expression levels of viral genes revealed disparities in plasmid derived gene expression between the cell lines. Further assessment of transfection efficiency utilizing labeled plasmids revealed lower plasmid uptake and less efficient nuclear transport in LP cell line. Additionally, we observed inferior translation activity in LP, contributing to its shortcomings in overall productivity. In our attempt to optimize plasmid ratios to enhance fully packaged rAAV particle yield, we discovered cell-line-specific optimization potential. The findings highlight the transfection's complexity, urging tailored strategies for improved rAAV production based on each cell line's characteristics, enhancing understanding and guiding further efficiency optimization in rAAV production.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Succession from acetoclastic to hydrogenotrophic microbial community during sewage sludge anaerobic digestion for bioenergy production 用于生物能源生产的污水污泥厌氧消化过程中从嗜醋微生物群落到嗜氢微生物群落的演替

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-09-11 DOI: 10.1007/s10529-024-03528-6

Mariana Erthal Rocha, Norberto Mangiavacchi, Marcia Marques, Lia Teixeira

{"title":"Succession from acetoclastic to hydrogenotrophic microbial community during sewage sludge anaerobic digestion for bioenergy production","authors":"Mariana Erthal Rocha, Norberto Mangiavacchi, Marcia Marques, Lia Teixeira","doi":"10.1007/s10529-024-03528-6","DOIUrl":"https://doi.org/10.1007/s10529-024-03528-6","url":null,"abstract":"To assess microbial dynamics during anaerobic digestion (AD) of sewage sludge (SWS) from a municipal Wastewater Treatment Plant (WWTP), a Biochemical Methane Potential (BMP) assay at 37 °C under mono-digestion conditions was conducted. Utilizing the Illumina MiSeq platform, 16S ribosomal RNA (rRNA) gene sequencing unveiled a core bacterial community in the solid material, showcasing notable variations in profiles. The research investigates changes in microbial communities and metabolic pathways to understand their impact on the efficiency of the digestion process. Prior to AD, the relative abundance in SWS was as follows: Proteobacteria > Bacteroidota > Actinobacteriota. Post-AD, the relative abundance shifted to Firmicutes > Synergistota > Proteobacteria, with Sporanaerobacter and Clostridium emerging as dominant genera. Notably, the methanogenic community underwent a metabolic pathway shift from acetoclastic to hydrogenotrophic in the lab-scale reactors. At the genus level, Methanosaeta, Methanolinea, and Methanofastidiosum predominated initially, while post-AD, Methanobacterium, Methanosaeta, and Methanospirillum took precedence. This metabolic transition may be linked to the increased abundance of Firmicutes, particularly Clostridia, which harbor acetate-oxidizing bacteria facilitating the conversion of acetate to hydrogen.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142224326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research on the application of heterotrophic nitrification-aerobic denitrification bacteria in membrane bioreactor (MBR) 异养硝化-好氧反硝化菌在膜生物反应器（MBR）中的应用研究

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-09-11 DOI: 10.1007/s10529-024-03529-5

Tianrui Zhai, Tiantao Zhao, Yuhao Zhong, Peipei Chen, Guojian Li, Liang Teng, Lijie Zhang, Hao Liu

引用次数: 0

Homologous expression of Aspergillus niger α-L-rhamnosidase and its application in enzymatic debittering of Ougan juice. 黑曲霉α-L-鼠李糖酶的同源表达及其在乌干果汁酶解脱盐中的应用。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-09-05 DOI: 10.1007/s10529-024-03531-x

Fei Zhang, Xue Wang, Lixia Pan, Zhao Wang, Jianyong Zheng

{"title":"Homologous expression of Aspergillus niger α-L-rhamnosidase and its application in enzymatic debittering of Ougan juice.","authors":"Fei Zhang, Xue Wang, Lixia Pan, Zhao Wang, Jianyong Zheng","doi":"10.1007/s10529-024-03531-x","DOIUrl":"https://doi.org/10.1007/s10529-024-03531-x","url":null,"abstract":"The α-L-rhamnosidase (rha1) gene was homologously expressed in Aspergillus niger strains CCTCC 206047 and CCTCC 206047ΔpyrG, using hygromycin B and auxotrophic as selection markers. The engineered A. niger strains RHA001-1 and RHA003-1 were screened, yielding α-L-rhamnosidase activities of 20.81 ± 0.56 U/mL and 15.35 ± 0.87 U/mL, respectively. The copy numbers of the rha1 gene in strains RHA001-1 and RHA003-1 were found to be 18 and 14, respectively. Correlation analysis between copy number and enzyme activity in the A. niger strains revealed that α-L-rhamnosidase activity increased with the copy number of the rha1 gene. Recombinant α-L-rhamnosidase was utilized for the enzymatic debittering of Ougan juice, and its process conditions were optimized. Furthermore, the primary bitter substance neohesperidin (2.22 g/L) in Ougan juice was converted into hesperetin 7-O-glucoside (1.47 g/L) and hesperidin (0.143 g/L). This study presents a novel approach for the production of food-grade α-L-rhamnosidase and establishes a technical foundation for its application in the beverage industry.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142131748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient RNA interference method by feeding in Brachionus plicatilis (Rotifera). 通过喂食对轮虫（Brachionus plicatilis）进行高效 RNA 干扰的方法。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-09-05 DOI: 10.1007/s10529-024-03524-w

Yu Zhang, Dongqi Kan, Yang Zhou, Hairong Lian, Lingling Ge, Jing Shen, Zhongqi Dai, Yan Shi, Cui Han, Xiaojie Liu, Jiaxin Yang

引用次数: 0