Biotechnology Letters最新文献_第2页

Cost-effective production of squalene using Yarrowia lipolytica via metabolic engineering and fermentation engineering. 通过代谢工程和发酵工程，利用聚脂耶氏菌经济高效地生产角鲨烯。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-05-05 DOI: 10.1007/s10529-025-03591-7

Wen-Bo Lin, Hong Chen, Ze-Qi Song, Yu-Qing Pan, Peng-Cheng Hu, Xiao-Na Yang, Xiang-Yang Lu, Yun Tian, Hu-Hu Liu

{"title":"Cost-effective production of squalene using Yarrowia lipolytica via metabolic engineering and fermentation engineering.","authors":"Wen-Bo Lin, Hong Chen, Ze-Qi Song, Yu-Qing Pan, Peng-Cheng Hu, Xiao-Na Yang, Xiang-Yang Lu, Yun Tian, Hu-Hu Liu","doi":"10.1007/s10529-025-03591-7","DOIUrl":"https://doi.org/10.1007/s10529-025-03591-7","url":null,"abstract":"Squalene is a triterpene with various biological applications. However, the conventioneer squalene industry is limited by complex extraction processes and environmental pollution, necessitating an environmentally sustainable solution to the increasing demand for squalene. Microbial synthesis is a potentially green and efficient method of producing squalene. Acetyl-CoA is a key precursor of squalene. First, we investigated the effects of enhanced acetyl-CoA supply on squalene production, lipid content, and total fatty acid content in Yarrowia lipolytica. Then, strain YLACLH2 with a squalene production of 232.29 mg/L was obtained by co-overexpressing YlACL2 and YlHMG1. Subsequently, the squalene production of YLACLH2 was increased to 514.33 mg/L by fermentation engineering, optimizing fermentation conditions including temperature, media volume, C/N ratio, shaker flask type and medium type. Finally, we investigated the synthesis efficiency of squalene in Y. lipolytica by acid-hydrolyzed sugarcane molasses (AHM) and waste cooking oil (WCO) as carbon sources with optimized fermentation conditions. This study showed that Y. lipolytica has the potential to produce squalene industrially using low-cost substrates. Our study findings provide reference for engineering Y. lipolytica to produce squalene using low-cost substrates and in an environmentally sustainable manner.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 3","pages":"49"},"PeriodicalIF":2.0,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic response of Bacillus spp. to heavy metal stress: pathway alterations and metabolite profiles. 芽孢杆菌对重金属胁迫的代谢反应：途径改变和代谢物谱。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-05-05 DOI: 10.1007/s10529-025-03589-1

Xiaowen Yang, Xiaotong Lin, Zhenglin Zhou, Bokun Lin, Xin Liu

{"title":"Metabolic response of Bacillus spp. to heavy metal stress: pathway alterations and metabolite profiles.","authors":"Xiaowen Yang, Xiaotong Lin, Zhenglin Zhou, Bokun Lin, Xin Liu","doi":"10.1007/s10529-025-03589-1","DOIUrl":"https://doi.org/10.1007/s10529-025-03589-1","url":null,"abstract":"Heavy metal pollution is a global issue that poses significant risks to ecosystems and human health. Microorganisms offer a promising bioremediation approach due to their ability to mitigate metal-induced metabolic damage in an eco-friendly, efficient, and cost-effective manner. Among them, Gram-positive Bacillus species exhibit a high heavy metal adsorption capacity and secrete metabolites with diverse functional properties. Under heavy metal stress, these metabolites play a crucial role in alleviating metal-induced damage. However, the application of Bacillus metabolites in heavy metal remediation faces challenges, including prolonged treatment durations, the necessity for stable environmental conditions, and specific nutrient requirements.This review summarizes recent research on the effects of heavy metal exposure on the metabolic pathways and metabolites of Bacillus spp., elucidates their role in influencing metal bioavailability and chemical transformations, and explores innovative strategies to enhance the stability of Bacillus-mediated heavy metal remediation. The review aims to provide valuable insights for optimizing bioremediation strategies, facilitating the selection of efficient degrading strains, and advancing the sustainable management of heavy metal contamination.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 3","pages":"50"},"PeriodicalIF":2.0,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of lactose based autoinduction for heterologous production of an active [NiFe] hydrogenase in E. coli. 乳糖自诱导在大肠杆菌中异种生产活性[NiFe]氢化酶中的应用。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-05-05 DOI: 10.1007/s10529-025-03594-4

Francisco de la Fuente-Kratzborn, Qin Fan, Peter Neubauer, Matthias Gimpel

引用次数: 0

Application of CRISPR-Cas9 in microbial cell factories. CRISPR-Cas9在微生物细胞工厂中的应用

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-04-21 DOI: 10.1007/s10529-025-03592-6

Jinhui Yang, Junyan Song, Zeyu Feng, Yunqi Ma

{"title":"Application of CRISPR-Cas9 in microbial cell factories.","authors":"Jinhui Yang, Junyan Song, Zeyu Feng, Yunqi Ma","doi":"10.1007/s10529-025-03592-6","DOIUrl":"https://doi.org/10.1007/s10529-025-03592-6","url":null,"abstract":"Metabolically engineered bacterial strains are rapidly emerging as a pivotal focus in the biosynthesis of an array of bio-based ingredients. Presently, CRISPR (clustered regularly interspaced short palindromic repeats) and its associated RNA-guided endonuclease (Cas9) are regarded as the most promising tool, having ushered in a transformative advancement in genome editing. Because of CRISPR-Cas9's accuracy and adaptability, metabolic engineers are now able to create novel regulatory systems, optimize pathways more effectively, and make extensive genome-scale alterations. Nevertheless, there are still obstacles to overcome in the application of CRISPR-Cas9 in novel microorganisms, particularly those industrial microorganism hosts that are resistant to traditional genetic manipulation techniques. How to further extend CRISPR-Cas9 to these microorganisms is an urgent problem to be solved. This article first introduces the mechanism and application of CRISPR-Cas9, and then discusses how to optimize CRISPR-Cas9 as a genome editing tool, including how to reduce off-target effects and how to improve targeting efficiency by optimizing design. Through offering a comprehensive perspective on the revolutionary effects of CRISPR-Cas9 in microbial cell factories, we hope to stimulate additional research and development in this exciting area.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 3","pages":"46"},"PeriodicalIF":2.0,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Creation of a highly stable direct electron transfer-type enzyme sensor by combining a hyperthermophilic dehydrogenase and natural electron mediator. 通过结合超嗜热脱氢酶和天然电子介质，创造出高度稳定的直接电子转移型酶传感器。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-04-21 DOI: 10.1007/s10529-025-03587-3

Miku Maeno, Haruhiko Sakuraba, Toshihisa Ohshima, Shin-Ichiro Suye, Takenori Satomura

{"title":"Creation of a highly stable direct electron transfer-type enzyme sensor by combining a hyperthermophilic dehydrogenase and natural electron mediator.","authors":"Miku Maeno, Haruhiko Sakuraba, Toshihisa Ohshima, Shin-Ichiro Suye, Takenori Satomura","doi":"10.1007/s10529-025-03587-3","DOIUrl":"https://doi.org/10.1007/s10529-025-03587-3","url":null,"abstract":"This study aimed to address the stability limitations of third-generation biosensors using enzymes from mesophilic organisms, by engineering a stable direct electron transfer (DET)-type dehydrogenase capable of transferring electrons extracted from the substrate to the electrode. A fusion protein combining the mediated electron transfer (MET)-type aldose sugar dehydrogenase from the hyperthermophile Pyrobaculum aerophilum (PaeASD), which cannot transfer electrons generated by enzymatic reactions to the electrode without a mediator, and the natural electron transfer protein cytochrome b562 (cyt b562) was developed to investigate its potential for the DET reaction. A recombinant protein expression system was established in Escherichia coli to produce the PaeASD-cyt b562 fusion protein, which was purified from the soluble fraction of the host cells. Intramolecular electron transfer from pyrroloquinoline quinone (PQQ) to the heme group within the PaeASD-cyt b562 fusion protein was investigated using UV-Vis absorption spectroscopy. Upon the addition of glucose, an increase in absorption corresponding to reduced heme molecules was observed, indicating electron transfer from glucose to the heme group in the cyt b562 component via PQQ in the PaeASD component. The DET capability of the fusion protein was evaluated using cyclic voltammetry with screen-printed carbon electrodes. A glucose concentration-dependent increase in current response confirmed DET activity. Notably, the fusion protein retained over 80% of its initial current response even after 2 months of storage at 4 °C. The novel robust PaeASD-cyt b562 fusion protein demonstrated efficient DET capability, highlighting its high potential for application in the development of third-generation biosensors.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 3","pages":"45"},"PeriodicalIF":2.0,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12011955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and application of expression constructs for FMDV serotype O structural proteins. FMDV血清O型结构蛋白表达构建体的设计与应用。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-04-21 DOI: 10.1007/s10529-025-03583-7

Mostafa R Zaher, Dalia M El-Husseini, Mohamed H El-Husseiny, Azza M El Amir, Naglaa M Hagag, Reham H Tammam

{"title":"Design and application of expression constructs for FMDV serotype O structural proteins.","authors":"Mostafa R Zaher, Dalia M El-Husseini, Mohamed H El-Husseiny, Azza M El Amir, Naglaa M Hagag, Reham H Tammam","doi":"10.1007/s10529-025-03583-7","DOIUrl":"https://doi.org/10.1007/s10529-025-03583-7","url":null,"abstract":"Design and validate flexible constructs for recombinant expression of FMDV serotype O structural proteins of the circulating topotypes using newly designed degenerate primers. The designed degenerate primers targeting diverse topotypes enabled the successful amplification of VP0, VP1, and VP3 genes. Integration of the essential transcriptional and translational regulatory elements including T7 promoter, leader g10 sequence, and T7 terminator, as well as ribosome binding site (RBS), start and stop codons, respectively via overlap extension PCR empowered efficient expression of these proteins in E. coli. Cloned constructs expressed the target proteins of expected molecular weights: VP0 (34 kDa), VP1 (24 kDa), and VP3 (22 kDa). SDS-PAGE and Western blotting confirmed high protein yield and purity. This platform demonstrated adaptability for diagnostic and vaccine development applications. The workflow offers a robust tool for producing FMDV structural proteins concerning the circulating strains attempting to improve control measures including diagnosis and vaccinations.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 3","pages":"44"},"PeriodicalIF":2.0,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction and immunogenicity analysis of a recombinant baculovirus targeting the N protein of SARS-CoV-2. 靶向SARS-CoV-2 N蛋白重组杆状病毒的构建及免疫原性分析。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-04-16 DOI: 10.1007/s10529-025-03576-6

Bohan Yu, Xiaoli Mo, Li Sui, Yujia Fang, Xudong Tang, Ping Qian

引用次数: 0

Network pharmacology, molecular docking, molecular dynamics simulation, and experiment verification analysis to reveal the action mechanism of RenShen Guipi Wan in the treatment of anemia. 网络药理学、分子对接、分子动力学模拟、实验验证分析揭示仁肾归脾丸治疗贫血的作用机制。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-04-16 DOI: 10.1007/s10529-025-03580-w

Tingli Qu, Nan Zhang, Chen Li, Xuyuan Liu, Keming Yun, Quan An

{"title":"Network pharmacology, molecular docking, molecular dynamics simulation, and experiment verification analysis to reveal the action mechanism of RenShen Guipi Wan in the treatment of anemia.","authors":"Tingli Qu, Nan Zhang, Chen Li, Xuyuan Liu, Keming Yun, Quan An","doi":"10.1007/s10529-025-03580-w","DOIUrl":"https://doi.org/10.1007/s10529-025-03580-w","url":null,"abstract":"Objective: To explore the action mechanisms of RGW that may treat anemia through the integration of network pharmacology, molecular docking, molecular dynamics simulation, and experiment verification.Result: In particular, Ginsenoside Rg4, Ginsenoside Rg1, 3,3',4,4'-Tetrahydroxy 2-methoxychalcone, Ginsenoside F1, Glycyrol, Chalconaringenin 4'-glucoside, Licochalcone B, 4',7-Dihydroxyflavone, Glycycoumarin, and Ginsenoside Rh1 were the core components, while TP53, STAT3, PIK3R1, SRC, HIF-1α were the core targets. The GO and KEGG analyses indicated that RGW may modulate multiple biological processes and pathways, including the PI3K-Akt, HIF-1, and NF-kappa B signaling pathways, as well as EGFR tyrosine kinase inhibitor resistance. Molecular docking and molecular dynamics simulations showed good affinity between the active components and core targets of RGW, with stable binding within 100 nano seconds. Experiment verification revealed RGW could improve the routine blood markers of mice, and decrease the level of HIF-1α significantly.Conclusion: RGW may treat anemia by regulating the PI3K-Akt and HIF-1 signaling pathways. It demonstrates the potential pharmacological mechanism of RGW in the treatment of anemia and provides a reference for clinical application of this formula.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 3","pages":"43"},"PeriodicalIF":2.0,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143966612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient and high-density immobilization of animal cells by a microfiber with both swelling and cell adhesion properties and its application to exosome production. 具有膨胀和细胞粘附性能的超细纤维高效高密度固定动物细胞及其在外泌体生产中的应用。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-04-12 DOI: 10.1007/s10529-025-03585-5

Naofumi Shiomi, Pengfei Zhang, Shuji Nakatsuka, Kazuo Kumagai, Hideto Matsuyama

{"title":"Efficient and high-density immobilization of animal cells by a microfiber with both swelling and cell adhesion properties and its application to exosome production.","authors":"Naofumi Shiomi, Pengfei Zhang, Shuji Nakatsuka, Kazuo Kumagai, Hideto Matsuyama","doi":"10.1007/s10529-025-03585-5","DOIUrl":"10.1007/s10529-025-03585-5","url":null,"abstract":"Purpose: For high-density cell culture, we studied the development of optimal microfibers (MFs) with a 0.1-10 μm diameter, which due to their large surface area can serve as an immobilization carrier for animal cells. To date, few studies have used MFs as scaffolding for high-density cell culturing.Results: Using six types of nonsoluble synthetic polymers, MF sheets were fabricated by electrospinning. The cellulose acetate, polyketone, and polyvinyl acetate MFs exhibited swelling and water retention capacities. Next, the six types of MF fragments were examined for immobilizing TKD2 mouse vascular endothelial cells. Although most cells were taken into the three MFs characterized by swelling, most leaked from the MFs without adhesion. To solve this, the MF sheets comprising cellulose acetate and polyketones were coated with gelatin. Although the adhesive capacity was enhanced, the swelling capacity decreased and almost all the immobilized mouse cells remained on the sheets' surfaces. Based on these results, we produced a novel MF sheet comprising a gelatin, cellulose acetate, and polyketone mixture (CPG). Since the cells were taken into the MFs by swelling and attached by the gelatin, the CPG fragment immobilized almost all the supplied cells with little loss and reached a high density of 3.2 × 109 MF-g-1, Furthermore, the immobilized cells continuously produced exosomes with a high productivity of 6-7 × 1010 particles ml-1 after either 8 h or 16 h of culturing.Conclusion: CPG-based MFs are expected to have a wide range of future applications, including exosome production from animal cells.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 2","pages":"40"},"PeriodicalIF":2.0,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11993475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering the Escherichia coli Nissle strain for monitoring the bacterial cell distribution and therapeutic protein expression within the intestinal tract of animal models. 设计大肠杆菌尼氏菌株，用于监测动物模型肠道内细菌细胞分布和治疗性蛋白表达。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-04-12 DOI: 10.1007/s10529-025-03586-4

Rui Gong, Yang Wu, Rushi Liu, Minjing Liao, Meifang Quan

{"title":"Engineering the Escherichia coli Nissle strain for monitoring the bacterial cell distribution and therapeutic protein expression within the intestinal tract of animal models.","authors":"Rui Gong, Yang Wu, Rushi Liu, Minjing Liao, Meifang Quan","doi":"10.1007/s10529-025-03586-4","DOIUrl":"10.1007/s10529-025-03586-4","url":null,"abstract":"The probiotic Escherichia coli Nissle 1917 (EcN) has been developed as a therapeutic carrier capable of enabling in vivo production of functional proteins. To optimize these processes, precise selection of promoters and monitoring of heterologous protein expression are important. Here, we designed a hypoxia-induced expression system in EcN by integrating a bicistronic cassette under the control of the Pvhb promoter. This construct enabled simultaneous transcription of oxdC (encoding oxalate decarboxylase, OxdC) and mCherry (a fluorescent reporter gene), achieving co-expression of both therapeutic and reporter proteins under hypoxic conditions. We confirmed that the Pvhb promoter efficiently initiated oxdC and mCherry co-expression under both in vitro hypoxic culture conditions and in vivo hypoxic environments within the intestinal tracts of animal models. Crucially, this system establishes mCherry as a noninvasive indicator for dual monitoring of probiotic localization and therapeutic protein expression within animal intestinal tracts. This work provides valuable insights for designing novel engineered bacteria for disease treatment.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 2","pages":"41"},"PeriodicalIF":2.0,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0