Biotechnology Letters最新文献_第3页

Characterization of the pesticidal crystal toxin protein Cry11Aa from Bacillus thuringiensis serovar israelensis VCRC B646 for mosquito larvae control. 苏云金芽孢杆菌（Bacillus thuringiensis serovar israelensis） VCRC B646杀虫晶体毒素蛋白Cry11Aa的鉴定

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2025-09-10 DOI: 10.1007/s10529-025-03640-1

Abhisubesh Vijayakumar, Sahadiya Mandodan, Jibi Lukose, Aneha Kunnikuruvan, Kakhuangailiu Gangmei, Bhagyashree Bora, Hemaladkshmi Padmanaban, Mathivanan Ashokkumar, Subbiah Poopathi

{"title":"Characterization of the pesticidal crystal toxin protein Cry11Aa from Bacillus thuringiensis serovar israelensis VCRC B646 for mosquito larvae control.","authors":"Abhisubesh Vijayakumar, Sahadiya Mandodan, Jibi Lukose, Aneha Kunnikuruvan, Kakhuangailiu Gangmei, Bhagyashree Bora, Hemaladkshmi Padmanaban, Mathivanan Ashokkumar, Subbiah Poopathi","doi":"10.1007/s10529-025-03640-1","DOIUrl":"https://doi.org/10.1007/s10529-025-03640-1","url":null,"abstract":"Effective mosquito control is essential for reducing the transmission of vector-borne diseases. This study focuses on the comprehensive characterization of mosquitocidal toxins produced by Bacillus thuringiensis serovar israelensis (Bti) VCRC B646 and the associated insecticidal genes. The bacterium was cultured, and the spore-crystal complex was purified to identify the mosquitocidal proteins. The mosquitocidal toxins produced by the isolate were evaluated against Aedes(Ae.) aegypti and Culex(Cx.) quinquefasciatus. SDS-PAGE and LC-MS were conducted to analyse the toxin, and PCR was performed to amplify the toxin gene. Toxicity bioassays indicated lethal concentrations (LC50 and LC90) for Ae. aegypti (0.0022 mg/L and 0.004 mg/L), and Cx, quinquefasciatus (0.0025 mg/L and 0.0044 mg/L). SDS-PAGE and LC-MS analysis revealed that Cry11Aa5 (Pesticidal Crystal Protein) is the predominant toxin produced by this strain. PCR amplification confirmed the presence of genes encoding various insecticidal proteins, including Cry and Cyt toxins. Phylogenetic analysis was performed to assess the genetic relatedness and toxin profiles of the bacterial isolate. This detailed characterization of Bti VCRC B646 highlights its potential as a promising biopesticide candidate for mosquito control, contributing to the development of sustainable and eco-friendly strategies for vector management.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 5","pages":"100"},"PeriodicalIF":2.1,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of a bacterial surface display system using split green fluorescent protein (GFP) in Escherichia coli. 利用裂解绿色荧光蛋白（GFP）在大肠杆菌中构建细菌表面显示系统。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2025-09-09 DOI: 10.1007/s10529-025-03633-0

Hae Won Kim, Jae Jung Kim, Jong-In Won

{"title":"Construction of a bacterial surface display system using split green fluorescent protein (GFP) in Escherichia coli.","authors":"Hae Won Kim, Jae Jung Kim, Jong-In Won","doi":"10.1007/s10529-025-03633-0","DOIUrl":"https://doi.org/10.1007/s10529-025-03633-0","url":null,"abstract":"The cell surface display system employs carrier proteins to present target proteins on the outer membrane of cells. This system enables functional proteins to be exposed on the exterior of living cells without cell lysis, allowing direct interaction with the surrounding environment. A major limitation of conventional approaches is the difficulty in displaying large-sized enzymes or antibodies, despite their critical roles in applications requiring functional domains that must remain intact, such as catalytic or antigen-binding sites. To address this challenge, we developed a novel system that enables the surface presentation of target proteins in Escherichia coli by integrating the cell surface display system with the self-assembly of split green fluorescent proteins (GFPs). In this system, GFP11M3 was fused to the carrier protein Lpp-OmpA and displayed on the bacterial surface. The surface-localized Lpp-OmpA-GFP11M3 subsequently assembled with GFP1-10opt, forming a functional GFP complex. By conjugating other target proteins, such as enzymes or antibodies, to GFP1-10opt, these biomolecules can be efficiently displayed on the cell surface. This approach not only facilitates the presentation of large biomolecules but also enables real-time visualization of protein localization through fluorescence detection.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 5","pages":"99"},"PeriodicalIF":2.1,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzymatic synthesis of inositol plasmalogens from distinct lipid sources using phospholipase D from Streptomyces antibioticus. 利用链霉菌的磷脂酶D从不同脂质来源合成肌醇缩醛原。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2025-09-08 DOI: 10.1007/s10529-025-03638-9

Shamoli Akter, Megumi Nishimukai, Miwa Yamada, Akiko Kashiwagi

{"title":"Enzymatic synthesis of inositol plasmalogens from distinct lipid sources using phospholipase D from Streptomyces antibioticus.","authors":"Shamoli Akter, Megumi Nishimukai, Miwa Yamada, Akiko Kashiwagi","doi":"10.1007/s10529-025-03638-9","DOIUrl":"https://doi.org/10.1007/s10529-025-03638-9","url":null,"abstract":"Plasmalogens are a subclass of glycerophospholipids characterized by a vinyl-ether bond at the sn-1 position; they play several physiological roles including membrane stabilization, antioxidant activity, and signal transduction. While choline, ethanolamine, serine, and glycerol plasmalogens (PlsCho, PlsEtn, PlsSer, and PlsGro) are naturally abundant, inositol plasmalogens (PlsIns) are rare. In contrast to the limited occurrence of PlsIns, phosphatidylinositol is a biologically crucial lipid, and its enzymatic biosynthesis from phosphatidylcholine has been extensively studied. Given that inositol itself is known to exert a range of physiological effects, it is reasonable to expect that PlsIns may also possess distinctive biological functions. Here, we report the first enzymatic synthesis of PlsIns using a phospholipase D-mediated transphosphatidylation reaction. Plasmalogen substrates-PlsEtn from Selenomonas ruminantium and both PlsEtn and PlsCho from chicken breast-were successfully converted to novel PlsIns species in the presence of myo-inositol. The resulting plasmalogens were detected by liquid chromatography-tandem mass spectrometry, confirming the introduction of the inositol moiety into the head group region. The results indicated that our method can be applied to different types of plasmalogens with different head groups and fatty acid profiles, including chain length and degree of unsaturation. This finding opens new avenues for exploring PlsIns and their potential biosignificance.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 5","pages":"98"},"PeriodicalIF":2.1,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fatty acid synthase in high and low lipid-producing strains of Mucor circinelloides: identification, phylogenetic analysis, and expression profiling during growth and lipid accumulation. 环状毛霉高脂和低脂产菌的脂肪酸合成酶：鉴定、系统发育分析和生长和脂质积累过程中的表达谱

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2025-09-07 DOI: 10.1007/s10529-025-03643-y

Yao Zhang, Yueping Yang, Zhuo Liu, Yan Sun, Aichun Guan, Qing Liu, Yuanda Song

{"title":"Fatty acid synthase in high and low lipid-producing strains of Mucor circinelloides: identification, phylogenetic analysis, and expression profiling during growth and lipid accumulation.","authors":"Yao Zhang, Yueping Yang, Zhuo Liu, Yan Sun, Aichun Guan, Qing Liu, Yuanda Song","doi":"10.1007/s10529-025-03643-y","DOIUrl":"https://doi.org/10.1007/s10529-025-03643-y","url":null,"abstract":"Fatty acid synthase (FAS) is one of the most important enzymes in lipid biosynthesis, which can catalyze the reaction of acetyl-CoA and malonyl-CoA to produce fatty acids. However, the structure, function, and molecular mechanism of FAS regulating lipid synthesis in the fungus Mucor circinelloides are unclear. In the present study, two encoding fas genes in the high lipid-producing strain WJ11 and low lipid-producing strain CBS277.49 from M. circinelloides based on their genome database were identified and annotated respectively. Bioinformatic analysis confirmed the presence of typical conserved domains in FAS proteins. Phylogenetic analysis revealed the evolutionary relationship of these FAS proteins, and the FAS proteins from WJ11 have similar properties as their counterparts in CBS277.49. Furthermore, transcriptional profiling displayed marked differences in the expression of these fas genes, and fas1 was analyzed to predict the possible functions in lipid metabolism in the high lipid-producing strain WJ11. This is the first report on the structures and functions of FAS proteins in M. circinelloides. This research has suggested the association of fas genes with lipid metabolism at the transcriptional level and contributed to the selection of candidates related to lipid metabolism for further study.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 5","pages":"97"},"PeriodicalIF":2.1,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A rapid and ultrasensitive CRISPR/Cas12a-based assay for the accurate identification of T-even type phages. 一种基于CRISPR/ cas12的快速、超灵敏的T-even型噬菌体准确鉴定方法。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2025-09-05 DOI: 10.1007/s10529-025-03634-z

Chenhang Jiang, Yang Li, Ping Yu, Mengjun Fang, Di Huang, Xiangming Fang, Zhinan Xu

{"title":"A rapid and ultrasensitive CRISPR/Cas12a-based assay for the accurate identification of T-even type phages.","authors":"Chenhang Jiang, Yang Li, Ping Yu, Mengjun Fang, Di Huang, Xiangming Fang, Zhinan Xu","doi":"10.1007/s10529-025-03634-z","DOIUrl":"https://doi.org/10.1007/s10529-025-03634-z","url":null,"abstract":"Phage contamination poses a significant threat to industrial fermentation, leading to substantial economic losses. Virulent T-even type phages (T2/T4/T6) represent particularly concerning biological hazards in fermentation systems. This paper developed a novel CRISPR/Cas12a-based system integrated with recombinase polymerase amplification (RPA), enabling ultrasensitive identification of T-even type phages. This method targeted the TerL gene of T-even type phages as a detection marker. The optimized RPA-CRISPR assay demonstrated exceptional sensitivity with a limit of detection (LOD) reaching 1 aM for synthetic targets. Besides, this system achieved detection thresholds of 1 and 10 PFU/μL for T2 and T4 phages, respectively. Comparative validation with quantitative PCR (qPCR) confirmed the method's reliability through strong correlation in the detection for both spiked and wastewater samples. The detection platform exhibited remarkable potential for rapid, sensitive monitoring of T-even type phages contamination in fermentation processes, offering promising application prospects for quality control in biochemical industries.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 5","pages":"95"},"PeriodicalIF":2.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The interaction between nitrogen source and light intensity affects the biomass and phenotypic plasticity of Scenedesmus obliquus. 氮源与光强的交互作用影响了斜叶麻的生物量和表型可塑性。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2025-09-05 DOI: 10.1007/s10529-025-03637-w

Jiyan Long, Yiqi Feng, Decai Huang, Yulu Lei, Xuexia Zhu, Zhou Yang

{"title":"The interaction between nitrogen source and light intensity affects the biomass and phenotypic plasticity of Scenedesmus obliquus.","authors":"Jiyan Long, Yiqi Feng, Decai Huang, Yulu Lei, Xuexia Zhu, Zhou Yang","doi":"10.1007/s10529-025-03637-w","DOIUrl":"https://doi.org/10.1007/s10529-025-03637-w","url":null,"abstract":"As critical environmental factors, nitrogen and light not only regulate phytoplankton growth but also influence their phenotypic plasticity. Scenedesmus obliquus, an alga which is famous for its remarkable phenotypic plasticity, was studied to understand its response to varying combinations of nitrogen source and light intensity. It was cultured in media containing different nitrogen sources (NaNO3, NH4Cl, CO(NH2)2) under a range of light intensities (25, 50, 75, 100, 150 µmol photons m-2 s-1). Results showed that growth rates increased with higher light intensities across all nitrogen sources. Photosynthetic efficiency (Fv/Fm and ΦPSII) remained stable in NaNO3 treatments, but declined with rising light intensity in NH4Cl and CO(NH2)2 treatments. The highest proportions of multicellular colonies were observed at 150 µmol photons m-2 s-1 for NH4Cl and NaNO3 treatments, while colonies in CO(NH2)2 treatments peaked at 100 µmol photons m-2 s-1, with colony size stabilized at approximately 2.1, 4.0, and 1.0 cells per particle under NaNO3, NH4Cl, and CO(NH2)2 treatments, respectively. Nitrogen removal efficiency improved with increasing light intensity across all treatments, though S. obliquus exhibited varying capacities to remove nitrogen depending on the sources. These findings demonstrated how S. obliquus adapts to varying nitrogen sources and light intensities in its growth, photosynthesis, and morphology, providing new evidence for our insights into its ecological versatility. This study established a theoretical foundation for optimizing culture conditions in applications such as wastewater treatment and bioenergy production.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 5","pages":"96"},"PeriodicalIF":2.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A functional and applied perspective of Vitreoscilla hemoglobin: from oxygen carriage to biotechnological innovation. 玻璃体颤菌血红蛋白的功能与应用：从载氧到生物技术创新。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2025-09-04 DOI: 10.1007/s10529-025-03635-y

Lianggang Huang, Yurong Li, Zhiqiang Liu, Yuguo Zheng

{"title":"A functional and applied perspective of Vitreoscilla hemoglobin: from oxygen carriage to biotechnological innovation.","authors":"Lianggang Huang, Yurong Li, Zhiqiang Liu, Yuguo Zheng","doi":"10.1007/s10529-025-03635-y","DOIUrl":"https://doi.org/10.1007/s10529-025-03635-y","url":null,"abstract":"Vitreoscilla hemoglobin (VHb), a homodimeric bacterial hemoglobin, exhibits distinct oxygen-binding properties that enhance cellular respiration and metabolic activity, particularly under hypoxic conditions. This review presents an updated and comprehensive synthesis of VHb-related research, encompassing its molecular structure, redox biochemistry, and transcriptional regulation. Compared with previous reviews, this work integrates recent mechanistic insights-especially those concerning transcription factor interactions, redox-coupled electron transfer, and structural-function relationships elucidated via targeted mutagenesis. In addition to its canonical role in oxygen delivery, VHb has been increasingly utilized in synthetic biology, high-cell-density fermentation, CRISPR-regulated expression platforms, and mammalian systems. Its biotechnological applications extend to enhancing microbial productivity under oxygen limitation, facilitating biocatalysis, and promoting biodegradation. In addition, the review highlights the emerging application of the vgb promoter as a strong regulatory element and summarizes current trends in VHb-related intellectual property and commercial development. VHb also shows promise in next-generation technologies such as environmental remediation and precision agriculture. Future directions should focus on optimizing expression systems, characterizing protein interaction networks, and engineering modular VHb-based components for advanced biosystems.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 5","pages":"94"},"PeriodicalIF":2.1,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Designing a multi-epitope vaccine against the midgut-specific fibrinogen-related protein 1(FREP1) of Anopheles stephensi to enhance protection against the malaria parasite: a step beyond traditional vaccine development approaches. 设计一种针对斯氏按蚊中肠特异性纤维蛋白原相关蛋白1（FREP1）的多表位疫苗，以增强对疟疾寄生虫的保护：超越传统疫苗开发方法的一步。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2025-09-03 DOI: 10.1007/s10529-025-03632-1

Mahima Yadav, Nisha Dahiya, Hitesh Singh, Divya Kataria, Sangeeta Janjoter, Neelam Sehrawat

{"title":"Designing a multi-epitope vaccine against the midgut-specific fibrinogen-related protein 1(FREP1) of Anopheles stephensi to enhance protection against the malaria parasite: a step beyond traditional vaccine development approaches.","authors":"Mahima Yadav, Nisha Dahiya, Hitesh Singh, Divya Kataria, Sangeeta Janjoter, Neelam Sehrawat","doi":"10.1007/s10529-025-03632-1","DOIUrl":"10.1007/s10529-025-03632-1","url":null,"abstract":"Malaria has been a prominent health burden for decades globally. The complex life cycle of Plasmodium made numerous challenges in finding an effective candidate for developing a potent transmission-blocking vaccine (TBV) against malaria. A wide variety of genes of Anopheles mosquitoes' midgut and salivary gland play a pivotal role in the Plasmodium invasion and transmission inside the mosquito body. Targeting mosquitoes' genes offered new insights into developing a more efficient TBV with higher potential to impede the parasite transmission within the Anopheles. Fibrinogen-related protein 1(FREP1) is a mosquito midgut protein that plays a crucial role in parasite transmission. In this study, we opted for an immunoinformatic approach to target An. stephensi FREP1 protein for breaking the parasite cycle so that the life cycle of the parasite could be broken within the mosquito. The FREP1 vaccine was assessed for allergenicity, antigenicity, toxicity, immunogenicity, population coverage, conservancy, solubility, secondary and tertiary structure, which suggested the impeccable quality of the vaccine construct. The interaction between the vaccine and TLR4 receptor via molecular docking revealed an efficient, strong, and stable complex formation. The molecular dynamic simulation and in-silico immunization profiling indicated the remarkable free binding energy and higher potency of the vaccine to generate a significant immune response, respectively. Furthermore, codon optimization and in-silico cloning of the vaccine in Escherichia coli exhibited efficient protein expression. In summary, the FREP1 protein-based multiepitope vaccine can be considered an innovative formulation for targeting the parasite within the vector to impede malaria transmission and vector control as well.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 5","pages":"93"},"PeriodicalIF":2.1,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intensification of interfacial enzymatic reactions in oil-water systems using slug flow in adaptive microfluidic channels. 自适应微流体通道中段塞流强化油水系统界面酶促反应。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2025-08-19 DOI: 10.1007/s10529-025-03631-2

Ruihao Zhu, Maojun Zhao, Xiaoyi Liu, Chen Chen, Haowen Zhu, Ting Guo, Tao Meng

{"title":"Intensification of interfacial enzymatic reactions in oil-water systems using slug flow in adaptive microfluidic channels.","authors":"Ruihao Zhu, Maojun Zhao, Xiaoyi Liu, Chen Chen, Haowen Zhu, Ting Guo, Tao Meng","doi":"10.1007/s10529-025-03631-2","DOIUrl":"10.1007/s10529-025-03631-2","url":null,"abstract":"Lipase is a type of hydrolase that catalyzes reactions at the water-in-oil (O/W) interface and possesses significant applied value across various fields. This study introduces integrated reaction-separation system employing microfluidic slug in a water-in-oil (W/O) droplet flow, specifically designed to enhance lipase-catalyzed interfacial lipid hydrolysis. By incorporating spiral microchannels, the system significantly improves interfacial mass transfer through slug flow-induced mixing and turbulence. Tributyrin hydrolysis within a liquid paraffin/phosphate buffer biphasic system serves as the model reaction to investigate the mechanisms underlying the intensification of interfacial enzymatic catalysis. Under comparable conditions, the microfluidic slug droplet system achieves an enzymatic reaction rate approximately 20 times greater than that observed in conventional beaker-based systems and 1.36 times greater than that in straight microchannels. The effects of droplet size, total flow rate, and channel curvature on conversion efficiency and reaction kinetics are examined, demonstrating that these parameters significantly impact mass transfer behavior. The dynamic interfaces generated within the slug flow architecture increase the specific surface area and facilitate accelerated mass transport, thereby enabling more efficient oil-water biphasic catalysis. This platform offers considerable potential for advancing interfacial biocatalysis and optimizing enzymatic transformations across a broad range of industrial and biotechnological applications.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 5","pages":"92"},"PeriodicalIF":2.1,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144871260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of various physicochemical parameters on the Biofilm formation and Pyocyanin production in Pseudomonas aeruginosa PA14. 不同理化参数对铜绿假单胞菌PA14生物膜形成及Pyocyanin生成的影响

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2025-08-15 DOI: 10.1007/s10529-025-03618-z

Sautrik Bhattacharya, Aravind Venkatasubramanian, Ananya Chatterjee, Partha Pratim Datta

{"title":"Effects of various physicochemical parameters on the Biofilm formation and Pyocyanin production in Pseudomonas aeruginosa PA14.","authors":"Sautrik Bhattacharya, Aravind Venkatasubramanian, Ananya Chatterjee, Partha Pratim Datta","doi":"10.1007/s10529-025-03618-z","DOIUrl":"10.1007/s10529-025-03618-z","url":null,"abstract":"Background: Biofilm formation in Pseudomonas aeruginosa provides protection against multiple stressors and contributes to its pathogenicity. Pyocyanin, a virulence factor regulated by quorum sensing, is crucial for infections. This study aimed to evaluate how various physicochemical conditions impact biofilm formation and pyocyanin production in P. aeruginosa PA14.Methods: Biofilm formation and pyocyanin production were assessed under varying conditions, including nutrient availability, NaCl concentrations, pH, temperature, heavy metal salts, light exposure, and microbial competition. Biofilm levels were quantified using a crystal violet assay, while pyocyanin levels were measured spectrophotometrically. Statistical analyses were performed to identify significant trends and correlations.Results: Key findings revealed that biofilm formation and pyocyanin production were reduced under most stress conditions examined in this study, compared to controls, with few exceptions. FeCl3 enhanced biofilm formation, while NaCl concentrations above 3% and extreme pH values inhibited it. NiCl2 was the most effective at reducing biofilm amount among the salts which we examined. Pyocyanin production followed similar trends, peaking under neutral pH and nutrient-enriched conditions. Positive correlations between biofilm and pyocyanin production were observed, particularly in nutrient-limited media. Additionally, light exposure and inter-microbial competition significantly reduced biofilm levels.Conclusion: This study highlights the differential responses of P. aeruginosa to various stress conditions, underscoring the importance of environmental factors in modulating biofilm formation and virulence. These findings provide insights into bacterial adaptive strategies and offer potential avenues for developing targeted interventions against biofilm-associated infections.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 5","pages":"91"},"PeriodicalIF":2.1,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0