Biotechnology Letters最新文献_第3页

Biomimetic mineralization of chemically modified glucose oxidase from Aspergillus niger in ZIF-8. 化学修饰的黑曲霉葡萄糖氧化酶在ZIF-8中的仿生矿化。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-03-14 DOI: 10.1007/s10529-026-03722-8

Marija Stanišić, Milica Crnoglavac Popović, Marko Radenković, Branimir Bajac, Vojin Tadić, Olivera Prodanović, Radivoje Prodanović

{"title":"Biomimetic mineralization of chemically modified glucose oxidase from Aspergillus niger in ZIF-8.","authors":"Marija Stanišić, Milica Crnoglavac Popović, Marko Radenković, Branimir Bajac, Vojin Tadić, Olivera Prodanović, Radivoje Prodanović","doi":"10.1007/s10529-026-03722-8","DOIUrl":"10.1007/s10529-026-03722-8","url":null,"abstract":"Chemical surface modification of enzymes represents a powerful strategy to improve immobilization and catalytic performance. In this study, glucose oxidase (GOx) from Aspergillus niger was chemically modified via periodate oxidation and reductive amination to incorporate L-aspartate (D-GOx) and L-histidine (H-GOx) residues. These modifications enhanced electrostatic and coordinative interactions with Zn2+ and 2-methylimidazole during biomimetic mineralization, leading to efficient encapsulation in ZIF-8 frameworks. The resulting D-GOx@ZIF-8 and H-GOx@ZIF-8 biocomposites showed significantly improved activity and stability compared to native GOx. H-GOx@ZIF-8 achieved a specific activity of 4551 IU/g, representing a sixfold increase over previously reported systems. Both modified biocomposites also demonstrated greater resistance to detergent washing and retained higher activity after exposure to 65 °C, indicating stronger incorporation into the ZIF-8 matrix. These results highlight the dual advantage of introducing negatively charged and imidazole-functional groups for promoting biomineralization and improving biocatalyst robustness. This strategy provides a mild, scalable route for preparing enzyme@MOF composites with enhanced operational stability, offering direct potential for industrial bioprocesses, continuous-flow catalysis, and biosensing applications.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147455502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antisense oligonucleotides: targeting oncogenic long non-coding RNAs for cancer therapy. 反义寡核苷酸：靶向致癌长链非编码rna用于癌症治疗。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-03-14 DOI: 10.1007/s10529-026-03713-9

Hamed Irandoost, Mahdieh Mondanizadeh, Elahe Irandoost

{"title":"Antisense oligonucleotides: targeting oncogenic long non-coding RNAs for cancer therapy.","authors":"Hamed Irandoost, Mahdieh Mondanizadeh, Elahe Irandoost","doi":"10.1007/s10529-026-03713-9","DOIUrl":"10.1007/s10529-026-03713-9","url":null,"abstract":"Purpose: This narrative review aims to summarize current evidence on antisense oligonucleotide (ASO)-based strategies targeting oncogenic long non-coding RNAs (lncRNAs) and to evaluate their therapeutic potential in cancer initiation, progression, metastasis, and treatment resistance.Methods: A narrative review of preclinical studies was conducted, focusing on ASO-mediated silencing of cancer-associated lncRNAs, including MALAT1, PVT1, NEAT1, and other emerging lncRNAs, across multiple malignancies such as lung, breast, ovarian, gastrointestinal, and head-and-neck cancers. Advances in ASO chemical design and delivery strategies-including lipid and polymeric nanoparticles, ligand-directed conjugates, peptide-based carriers, and gymnotic uptake-were also examined.Results: Preclinical evidence consistently demonstrates that lncRNA-targeted ASOs effectively suppress tumor growth, invasion, metastasis, and chemoresistance while promoting apoptosis. Improvements in ASO chemical modifications and delivery platforms have significantly enhanced ASO stability, biodistribution, and intracellular uptake, thereby improving therapeutic efficacy across diverse cancer models.Conclusion: Targeting oncogenic lncRNAs using ASO-based approaches represents a promising and rapidly evolving strategy in precision oncology. Although substantial progress has been achieved in ASO design and delivery, further optimization and well-structured clinical trials are required to facilitate the translation of these therapies into routine cancer treatment.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147455437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant-derived vaccines for veterinary disease management in ruminants and swine. 反刍动物和猪兽医疾病管理用植物源性疫苗。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-03-06 DOI: 10.1007/s10529-026-03715-7

Balamurugan Shanmugaraj, Abdul Basith Akbar Aly, Chanjetha Murugan, Kiruthika Jayaraj, Sathishkumar Ramalingam

{"title":"Plant-derived vaccines for veterinary disease management in ruminants and swine.","authors":"Balamurugan Shanmugaraj, Abdul Basith Akbar Aly, Chanjetha Murugan, Kiruthika Jayaraj, Sathishkumar Ramalingam","doi":"10.1007/s10529-026-03715-7","DOIUrl":"10.1007/s10529-026-03715-7","url":null,"abstract":"Vaccination remains one of the effective strategies to control infectious diseases, thereby preventing millions of deaths each year globally. Veterinary vaccines protects animal and public health, enabling efficient production of livestock, reduce antibiotic use and also prevent zoonotic spill over events. Traditional vaccines were developed using live-attenuated or inactivated pathogens. However, the advancements in the genetic engineering and recombinant protein production technologies have made possible the vaccine design and production of recombinant vaccine antigens or immunotherapeutic molecules for both human and veterinary use. Most of the commercially available recombinant therapeutic proteins are produced in mammalian system which limits their feasibility, particularly for veterinary applications due to their high production costs. Therefore, the development of low cost products using alternative production platforms is highly essential. The utilization of plant expression system for the recombinant vaccine production has increased in the recent years due to its advantages such as low production costs, scalability, safety, and the ability to express complex proteins. This review provides a comprehensive overview of the vaccine antigens produced in plant system with a particular focus on vaccines targeting infectious diseases in ruminants and swine.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147368867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immobilization of hydroaminase-expressing recombinant Escherichia coli whole-cell biocatalysts for the production of β-alanine. 表达氢胺酶的重组大肠杆菌全细胞生物催化剂的固定化生产β-丙氨酸。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-03-06 DOI: 10.1007/s10529-026-03705-9

Li Ma, Yuyu Wang, Ruiqi Liu, Jingjia Hu, Xiaobing Zheng, Xiaoyang Yue, Guanhua Liu, Ying He, Liya Zhou

{"title":"Immobilization of hydroaminase-expressing recombinant Escherichia coli whole-cell biocatalysts for the production of β-alanine.","authors":"Li Ma, Yuyu Wang, Ruiqi Liu, Jingjia Hu, Xiaobing Zheng, Xiaoyang Yue, Guanhua Liu, Ying He, Liya Zhou","doi":"10.1007/s10529-026-03705-9","DOIUrl":"10.1007/s10529-026-03705-9","url":null,"abstract":"Developing stable and easy-to-operate biocatalysts is crucial for their use as industrial catalysts. Here immobilized whole-cell catalysts were used for β-alanine production by immobilizing recombinant Escherichia coli cells (containing hydroaminase) with diatomite. E. coli BL21 (DE3)/pET-30a ( +)-HAMase was genetically engineered for the efficient synthesis of β-alanine from acrylic acid and aqueous ammonia. Using glutaraldehyde as a cross-linking agent, polyethyleneimine (PEI) as a flocculant, and diatomite as the immobilization carrier, optimal immobilization was achieved with 8% (w/v) PEI solution, 5% (w/v) glutaraldehyde, and 100 mg wet cell/mL cell suspension, along with a PEI flocculation time of 2.5 h and glutaraldehyde cross-linking time of 1.5 h. The enzyme activity recovery rate reached 70.72%. Remarkably, the immobilized whole-cell catalysts exhibited excellent stability, retaining over 90% of initial enzyme activity after 12 h incubation at 45 °C and maintaining over 72% enzyme activity after storage for 60 days at 4 °C. Additionally, the immobilized cells demonstrated enhanced reusability, maintaining consistent β-alanine yield even after ten consecutive reaction batches with an average yield of approximately 80%.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147363930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A rapid analytical method using loop-mediated isothermal amplification (LAMP): visual detection of cry1Ab gene in genetically modified maize seeds and food products. 环介导等温扩增（LAMP）快速检测转基因玉米种子及食品中cry1Ab基因

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-03-05 DOI: 10.1007/s10529-026-03716-6

Monika Singh, Shiwani, Kushaldeep Kaur, Divyaditya Awasthi, Amit Kumar Singh

{"title":"A rapid analytical method using loop-mediated isothermal amplification (LAMP): visual detection of cry1Ab gene in genetically modified maize seeds and food products.","authors":"Monika Singh, Shiwani, Kushaldeep Kaur, Divyaditya Awasthi, Amit Kumar Singh","doi":"10.1007/s10529-026-03716-6","DOIUrl":"10.1007/s10529-026-03716-6","url":null,"abstract":"Insect resistance is a predominant trait in the globally approved genetically modified (GM) crops. The cry1Ab transgene is featured in over 25% of globally approved insect resistant GM events including both single as well as stacked trait events. GM maize notably contributes for 87% among the approved GM events with cry1Ab gene. GM detection of cry1Ab gene in maize seeds and food products is important for authentication purpose in the countries where GM maize events with cry1Ab gene such as Bt11, Bt176, MON810 are approved, and for surveillance of unauthorized occurrence of such events in the countries where these are not approved. Cost-efficient and rapid GM detection methods are essential for decentralized monitoring for unauthorized GM events across agricultural fields, border inspections, and food supply chains, and for regulatory compliance enabling quick decision-making. A rapid GM detection method employing loop-mediated isothermal amplification (LAMP) was developed for visual analysis of cry1Ab gene in GM maize. The developed assay showed acceptable specificity, which could reliably detect as low as 0.005% of cry1Ab within 60 min. Practical applicability of this assay was also verified for GM detection in maize containing products. The developed method offers a convenient, rapid and cost-effective tool for checking the cry1Ab gene either for regulatory purpose in the countries where the GM crops with cry1Ab gene are restricted or for confirmatory purpose for the samples with cry1Ab gene whenever required.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147363889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced gibberellic acid production in Fusarium fujikuroi through ARTP-EMS mutagenesis and fermentation optimization. 通过ARTP-EMS诱变和发酵优化提高藤黑镰刀菌的赤霉素产量。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-03-02 DOI: 10.1007/s10529-026-03699-4

Yuan-Shan Wang, Zhi-Tao Dong, Ke-Lei Dai, Lang Wang, Chun-Yue Weng, Yu-Ke Cen, Zhi-Qiang Liu, Yu-Guo Zheng

引用次数: 0

Molecular determinants of the immunomodulatory potentials of a novel bifunctional TGF-β1/PD-L1 fusion protein. 一种新型双功能TGF-β1/PD-L1融合蛋白免疫调节潜能的分子决定因素。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-02-23 DOI: 10.1007/s10529-026-03711-x

Marvin I De Los Santos

{"title":"Molecular determinants of the immunomodulatory potentials of a novel bifunctional TGF-β1/PD-L1 fusion protein.","authors":"Marvin I De Los Santos","doi":"10.1007/s10529-026-03711-x","DOIUrl":"10.1007/s10529-026-03711-x","url":null,"abstract":"Autoimmune diseases (AIDs) arise from loss of immune tolerance and are commonly treated with broad immunosuppressive drugs that increase the risk of infection and malignancy. Transforming growth factor-beta 1 (TGF-β1) and programmed death-ligand 1 (PD-L1) are two immunoregulatory proteins whose receptors are expressed on effector and regulatory T cells, and independently modulate immune functions. However, the use of these ligands as a bifunctional regulatory T cell engager (BiTE-like) remains unexplored. This study aimed to design, express, and functionally characterize the development of a TGF-β1/PD-L1 fusion protein. The fusion construct was engineered using overlap-extension PCR and cloned into a mammalian expression vector (pCDNA3.1 +). Recombinant expression in HEK 293 cells resulted in a ~ 70 kDa protein, verified using SDS-PAGE, immunoprecipitation, and western blot. ELISA, co-immunoprecipitation (co-IP) and reverse co-IP experiments confirmed that the TGF-β1 domain in the fusion protein required co-receptors to bind TGFβ-R1, consistent with the canonical receptor binding mechanism of endogenous TGF-β proteins. Parallel immunoassays demonstrated specific binding between the PD-L1 domain and PD-1 receptor. Functional validation in vitro showed differential expression of TGF-β1 and PD-L1 responsive genes, including c-Myc and IFN-γ, upon treatment of leukemic cell model with the fusion protein. SEAP-based reporter assay confirmed the ability of TGF-β1/PD-L1 fusion protein to inhibit NF-κB activation. These results suggested the functional and active binding of TGF-β1 and PD-L1 domains with their cognate receptors. Collectively, this study provided molecular evidence for the structural and functional integrity of the TGF-β1/PD-L1 fusion protein and support its potential development for targeted immunomodulation in AIDs.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147275577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in genome-scale metabolic modeling of Bacillus subtilis. 枯草芽孢杆菌基因组代谢模型的研究进展。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-02-23 DOI: 10.1007/s10529-026-03712-w

Pınar Kocabaş

引用次数: 0

Dual functions of nonionic surfactant Tween-20 in enhancing productivity and elution efficiency of alkylresorcinols from Azotobacter vinelandii. 非离子表面活性剂Tween-20在提高固氮杆菌产率和洗脱效率中的双重作用。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-02-17 DOI: 10.1007/s10529-026-03709-5

Rahul Jog, Shigeru Hiramoto, Yasushi Ohgo, Masaaki Morikawa

{"title":"Dual functions of nonionic surfactant Tween-20 in enhancing productivity and elution efficiency of alkylresorcinols from Azotobacter vinelandii.","authors":"Rahul Jog, Shigeru Hiramoto, Yasushi Ohgo, Masaaki Morikawa","doi":"10.1007/s10529-026-03709-5","DOIUrl":"10.1007/s10529-026-03709-5","url":null,"abstract":"Alkylresorcinols (AR) are a class of phenolic lipids widely distributed in plants, algae, fungi, and bacteria. AR are high-value bioproducts with applications in a broad range of industries, ranging from agriculture to healthcare. They are, however, hydrophobic and found in trace amounts, especially in hard grain bran, and thus require intensive mechanical crushing and harmful organic solvent-based processes for elution. The aim of this study is to demonstrate a proof-of-concept of an innovative AR production and extraction technology to ensure hygienic and safe working environments. In this study, we examined the competency of AR production by diazotrophic bacteria Azotobacter vinelandii using various surfactants. The effect of selected surfactant was tested not only at the stage of AR elution from cysts but also during the cell culture forming cysts. It revealed that nonionic surfactants, Tween-20 and Tween-80, were capable of not only solubilizing AR from the mature cysts of A. vinelandii but also increasing their production during cyst formation. The production yield of AR in soluble form maximally reached 5.5 mg/L in Burk's nitrogen-free liquid medium containing 0.2% n-butanol and 0.1% Tween-20. These findings contribute to the development of an efficient and solvent-free green production method for AR.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 2","pages":"39"},"PeriodicalIF":2.1,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146211970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombinant erythropoietin expression elevates by ubiquitous chromatin opening element (UCOE) in CHO DG44 cells. 重组红细胞生成素在CHO DG44细胞中通过普遍存在的染色质开放元件（UCOE）表达升高。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-02-16 DOI: 10.1007/s10529-026-03707-7

Fateme Hasheminejad, Haniyeh Norouzi, Seyede Hoda Jazayeri, Zahra Halfinezhad, Abbas Daneshipour, Zeynab Khoshnood, Mahsa Nejati, Fatemeh Norouzi, Somayeh Abolghasemi, Mohsen Gharanfoli, Sara Taleahmad, Amir Amiri-Yekta

{"title":"Recombinant erythropoietin expression elevates by ubiquitous chromatin opening element (UCOE) in CHO DG44 cells.","authors":"Fateme Hasheminejad, Haniyeh Norouzi, Seyede Hoda Jazayeri, Zahra Halfinezhad, Abbas Daneshipour, Zeynab Khoshnood, Mahsa Nejati, Fatemeh Norouzi, Somayeh Abolghasemi, Mohsen Gharanfoli, Sara Taleahmad, Amir Amiri-Yekta","doi":"10.1007/s10529-026-03707-7","DOIUrl":"10.1007/s10529-026-03707-7","url":null,"abstract":"The growing demand for biopharmaceuticals has increased the need for efficient and cost-effective recombinant protein production platforms. Transcriptional gene silencing after random genomic integration of expression vectors remains a major limitation in mammalian expression systems and often reduces protein yield. Incorporation of ubiquitous chromatin opening elements (UCOEs) into expression cassettes has emerged as a promising strategy to mitigate position effects and enhance transgene expression. This study examined the effect of a UCOE-containing expression system on erythropoietin (EPO) production in Chinese hamster ovary (CHO) DG44 cells. A codon-optimized EPO expression cassette was introduced into CHO DG44 cells using either a conventional pOptiVEC vector and a UCOE-containing vector following random genomic integration. Recombinant EPO expression was assessed at the transcriptional and protein levels using reverse transcription quantitative polymerase chain reaction (RT-qPCR), western blotting, and enzyme-linked immunosorbent assay (ELISA). The UCOE-containing cell pool exhibited a significant enhancement in recombinant EPO expression, with approximately a 3.8-fold increase in mRNA levels and a sevenfold increase in secreted protein levels compared to the non-UCOE control cell pool. These results show that incorporating a UCOE reduces position-dependent gene silencing and increases recombinant mRNA and protein expression in CHO DG44 cell pools. This strategy supports improved efficiency during the early stages of cell line development for recombinant protein production.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 2","pages":"38"},"PeriodicalIF":2.1,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146200095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0