Biotechnology Letters最新文献_第4页

Isolation of a facultative methanotroph Methylocystis iwaonis SD4 from rice rhizosphere and establishment of rapid genetic tools for it. 从水稻根瘤中分离出一种兼性甲烷营养体 Methylocystis iwaonis SD4，并为其建立快速遗传工具。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-05-11 DOI: 10.1007/s10529-024-03495-y

Yinghui Wang, Yuying Wang, Keyu Zhou, Haili Zhang, Minggen Cheng, Baozhan Wang, Xin Yan

{"title":"Isolation of a facultative methanotroph Methylocystis iwaonis SD4 from rice rhizosphere and establishment of rapid genetic tools for it.","authors":"Yinghui Wang, Yuying Wang, Keyu Zhou, Haili Zhang, Minggen Cheng, Baozhan Wang, Xin Yan","doi":"10.1007/s10529-024-03495-y","DOIUrl":"10.1007/s10529-024-03495-y","url":null,"abstract":"Methanotrophs of the genus Methylocystis are frequently found in rice paddies. Although more than ten facultative methanotrophs have been reported since 2005, none of these strains was isolated from paddy soil. Here, a facultative methane-oxidizing bacterium, Methylocystis iwaonis SD4, was isolated and characterized from rhizosphere samples of rice plants in Nanjing, China. This strain grew well on methane or methanol but was able to grow slowly using acetate or ethanol. Moreover, strain SD4 showed sustained growth at low concentrations of methane (100 and 500 ppmv). M. iwaonis SD4 could utilize diverse nitrogen sources, including nitrate, urea, ammonium as well as dinitrogen. Strain SD4 possessed genes encoding both the particulate methane monooxygenase and the soluble methane monooxygenase. Simple and rapid genetic manipulation methods were established for this strain, enabling vector transformation and unmarked genetic manipulation. Fast growth rate and efficient genetic tools make M. iwaonis SD4 an ideal model to study facultative methanotrophs, and the ability to grow on low concentration of methane implies its potential in methane removal.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140908047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized detection of Salmonella typhimurium using aptamer lateral flow assay. 利用aptamer横向流动检测法优化鼠伤寒沙门氏菌的检测。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-05-28 DOI: 10.1007/s10529-024-03484-1

Nafise Abedi, Mehdi Zeinoddini, Mohammad Shoushtari

{"title":"Optimized detection of Salmonella typhimurium using aptamer lateral flow assay.","authors":"Nafise Abedi, Mehdi Zeinoddini, Mohammad Shoushtari","doi":"10.1007/s10529-024-03484-1","DOIUrl":"10.1007/s10529-024-03484-1","url":null,"abstract":"Salmonella typhimurium, a pathogenic bacterium with significant implications in medicine and the food industry, poses a substantial threat by causing foodborne illnesses such as typhoid fever. Accurate diagnosis of S. typhimurium is challenging due to its overlap symptoms with various diseases. This underscores the need for a precise and efficient diagnostic approach. In this study, we developed a biosensor using the Taguchi optimization method based on aptamer lateral flow assay (LFA) for the detection of S. typhimurium. Therefore, signal probe and nanobioprobe were designed using anti-Salmonella aptamer, conjugated with gold nanoparticles (GNPs), and used in LFA. The strategy of this test is based on a competitive format between the bacteria immobilized on the membrane and the bacteria present in the tested sample. Moreovere, the optimization of various factors affecting the aptamer LFA, including the concentration of bacteria (immobilized and into the sample) and the concentration of nanobioprop, were performed using the Taguchi test designing method. The data showed that the optimal conditions for the LFA reaction was 108 CFU/mL of immobilized bacteria and 1.5 μg/μL of nanobioprop concentration. Then, the visual detection limit of S. typhimurium was estimated as 105 CFU/mL. The reaction results were obtained within 20 min, and there were no significant cross-reactions with other food pathogens. In conclusion, the aptamer-LFA diagnostic method, optimized using the Taguchi approach, emerges as a reliable, straightforward, and accurate tool for the detection of S. typhimurium. Overall, this method can be a portable diagnostic kit for the detection and identification of bacteria.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141160106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paddy straw saccharification using immobilized laccase on magnetized multiwall carbon nanotubes. 利用磁化多壁碳纳米管上的固定化漆酶对稻草进行糖化。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-05-15 DOI: 10.1007/s10529-024-03494-z

Hasnol Azahari Natasha Yasmin, Balakrishnan Kunasundari, Siew Hoong Shuit, Mohamad Fahrurrazi Tompang

{"title":"Paddy straw saccharification using immobilized laccase on magnetized multiwall carbon nanotubes.","authors":"Hasnol Azahari Natasha Yasmin, Balakrishnan Kunasundari, Siew Hoong Shuit, Mohamad Fahrurrazi Tompang","doi":"10.1007/s10529-024-03494-z","DOIUrl":"10.1007/s10529-024-03494-z","url":null,"abstract":"The effective recovery of the immobilized enzymes using magnetic carriers has led to growing interest in this technology. The objective of this research was to evaluate the efficiency of immobilized laccase on magnetized multiwall carbon nanotubes (m-MWCNTs) in terms of stability and reusability. Laccases were efficiently adsorbed onto magnetized multiwall carbon nanotubes (m-MWCNTs) synthesized using water. The concentration of 7 mg laccase/mL was found to be ideal for immobilization. The optimal activity of both free and immobilized laccases was observed at pH 5, while for the latter, the optimal temperature was shifted from 40 to 50 °C. Compared to the free laccase, the immobilized laccase exhibited a greater range of stability at more extreme temperatures. At the fourth cycle of reactions, the immobilized laccase exhibited more than 60% relative activity in terms of reusability. Based on the fourier-transform infrared spectroscopy (FTIR) peak at 2921 cm-1, saccharification of paddy straw using immobilized laccase verified lignin degradation. The easy recovery of the immobilized laccase on m-MWCNTs lends credence to its potential use in biomass hydrolysis.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140921166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning: an advancement in biochemical engineering. 机器学习：生物化学工程的进步。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-06-21 DOI: 10.1007/s10529-024-03499-8

Ritika Saha, Ashutosh Chauhan, Smita Rastogi Verma

引用次数: 0

Isolation and characterization of bio-prospecting gut strains Bacillus safensis CGK192 and Bacillus australimaris CGK221 for plastic (HDPE) degradation. 分离和鉴定用于降解塑料（高密度聚乙烯）的生物探究性肠道菌株 Bacillus safensis CGK192 和 Bacillus australimaris CGK221。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-05-06 DOI: 10.1007/s10529-024-03486-z

Kamal Kant Sharma, Himalaya Panwar, Kartikey Kumar Gupta

{"title":"Isolation and characterization of bio-prospecting gut strains Bacillus safensis CGK192 and Bacillus australimaris CGK221 for plastic (HDPE) degradation.","authors":"Kamal Kant Sharma, Himalaya Panwar, Kartikey Kumar Gupta","doi":"10.1007/s10529-024-03486-z","DOIUrl":"10.1007/s10529-024-03486-z","url":null,"abstract":"The present work reports the application of novel gut strains Bacillus safensis CGK192 (Accession No. OM658336) and Bacillus australimaris CGK221 (Accession No. OM658338) in the biological degradation of synthetic polymer i.e., high-density polyethylene (HDPE). The biodegradation assay based on polymer weight loss was conducted under laboratory conditions for a period of 90 days along with regular evaluation of bacterial biomass in terms of total protein content and viable cells (CFU/cm2). Notably, both strains achieved significant weight reduction for HDPE films without any physical or chemical pretreatment in comparison to control. Hydrophobicity and biosurfactant characterization were also done in order to assess strains ability to form bacterial biofilm over the polymer surface. The post-degradation characterization of HDPE was also performed to confirm degradation using analytical techniques such as Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Field emission scanning electronic microscopy (FE-SEM) coupled with energy dispersive X-ray (EDX), and Gas chromatography-mass spectrometry (GC-MS). Interestingly strain CGK221 was found to be more efficient in forming biofilm over polymer surface as indicated by lower half-life (i.e., 0.00032 day-1) and higher carbonyl index in comparison to strain CGK192. The findings reflect the ability of our strains to develop biofilm and introduce an oxygenic functional group into the polymer surface, thereby making it more susceptible to degradation.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140851319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The modification of heme special importer to improve the production of active hemoglobins in Escherichia coli. 改造血红素特殊导入器，提高大肠杆菌活性血红蛋白的产量。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-05-08 DOI: 10.1007/s10529-024-03488-x

Zihan Zhang, Baodong Hu, Tao Zhang, Zhengshan Luo, Jingwen Zhou, Jianghua Li, Jian Chen, Guocheng Du, Xinrui Zhao

{"title":"The modification of heme special importer to improve the production of active hemoglobins in Escherichia coli.","authors":"Zihan Zhang, Baodong Hu, Tao Zhang, Zhengshan Luo, Jingwen Zhou, Jianghua Li, Jian Chen, Guocheng Du, Xinrui Zhao","doi":"10.1007/s10529-024-03488-x","DOIUrl":"10.1007/s10529-024-03488-x","url":null,"abstract":"To enhance the import of heme for the production of active hemoproteins in Escherichia coli C41 (DE3) lacking the special heme import system, heme receptor ChuA from E. coli Nissle 1917 was modified through molecular docking and the other components (ChuTUV) for heme import was overexpressed, while heme import was tested through growth assay and heme sensor HS1 detection. A ChuA mutant G360K was selected, which could import 3.91 nM heme, compared with 2.92 nM of the wild-type ChuA. In addition, it presented that the expression of heme transporters ChuTUV was not necessary for heme import. Based on the modification of ChuA (G360K), the titer of human hemoglobin and the peroxidase activity of leghemoglobin reached 1.19 μg g-1 DCW and 24.16 103 U g-1 DCW, compared with 1.09 μg g-1 DCW and 21.56 103 U g-1 DCW of the wild-type ChuA, respectively. Heme import can be improved through the modification of heme receptor and the engineered strain with improved heme import has a potential to efficiently produce high-active hemoproteins.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140875754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of fadR on the ROS defense mechanism in Shewanalla oneidensis. fadR 对 Shewanalla oneidensis 中 ROS 防御机制的调控。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-05-06 DOI: 10.1007/s10529-024-03487-y

Qiu Meng, Yinming Xu, Liming Dai, Xuzhe Ge, Pei Qiao

引用次数: 0

Preparation of (S)-epichlorohydrin using a novel halohydrin dehalogenase by selective conformation adjustment. 利用新型卤代卤素脱卤酶通过选择性构象调整制备 (S)-环氧氯丙烷。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-05-11 DOI: 10.1007/s10529-024-03479-y

Xiao-Jian Zhang, Meng-Yu Huang, Xin-Xin Peng, Min Cao, Han-Zhong Deng, Yi-Chuan Gong, Xiao-Ling Tang, Zhi-Qiang Liu, Yu-Guo Zheng

{"title":"Preparation of (S)-epichlorohydrin using a novel halohydrin dehalogenase by selective conformation adjustment.","authors":"Xiao-Jian Zhang, Meng-Yu Huang, Xin-Xin Peng, Min Cao, Han-Zhong Deng, Yi-Chuan Gong, Xiao-Ling Tang, Zhi-Qiang Liu, Yu-Guo Zheng","doi":"10.1007/s10529-024-03479-y","DOIUrl":"10.1007/s10529-024-03479-y","url":null,"abstract":"Chiral epichlorohydrin (ECH) is an attractive intermediate for chiral pharmaceuticals and chemicals preparation. The asymmetric synthesis of chiral ECH using 1,3-dicholoro-2-propanol (1,3-DCP) catalyzed by a haloalcohol dehalogenase (HHDH) was considered as a feasible approach. However, the reverse ring opening reaction caused low optical purity of chiral ECH, thus severely restricts the industrial application of HHDHs. In the present study, a novel selective conformation adjustment strategy was developed with an engineered HheCPS to regulate the kinetic parameters of the forward and reverse reactions, based on site saturation mutation and molecular simulation analysis. The HheCPS mutant E85P was constructed with a markable change in the conformation of (S)-ECH in the substrate pocket and a slight impact on the interaction between 1,3-DCP and the enzyme, which resulted in the kinetic deceleration of the reverse reactions. Compared with HheCPS, the catalytic efficiency (kcat(S)-ECH/Km(S)-ECH) of the reversed reaction dropped to 0.23-fold (from 0.13 to 0.03 mM-1 s-1), while the catalytic efficiency (kcat(1,3-DCP)/Km(1,3-DCP)) of the forward reaction only reduced from 0.83 to 0.71 mM-1 s-1. With 40 mM 1,3-DCP as substrate, HheCPS E85P catalyzed the synthesis of (S)-ECH with the yield up to 55.35% and the e.e. increased from 92.54 to >99%. Our work provided an effective approach for understanding the stereoselective catalytic mechanism as well as the green manufacturing of chiral epoxides.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140908122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering of Saccharomyces cerevisiae for high-level production of (+)-ambrein from glucose. 利用代谢工程改造酿酒酵母，从葡萄糖中高水平生产 (+)-ambrein 蛋白。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-06-17 DOI: 10.1007/s10529-024-03502-2

Chumin Lin, Xiaopeng Zhang, Zhongju Ji, Baolian Fan, Yaman Chen, Yuhong Wu, Yuhong Gan, Zhengping Li, Yi Shang, Lixin Duan, Feng Wang

{"title":"Metabolic engineering of Saccharomyces cerevisiae for high-level production of (+)-ambrein from glucose.","authors":"Chumin Lin, Xiaopeng Zhang, Zhongju Ji, Baolian Fan, Yaman Chen, Yuhong Wu, Yuhong Gan, Zhengping Li, Yi Shang, Lixin Duan, Feng Wang","doi":"10.1007/s10529-024-03502-2","DOIUrl":"10.1007/s10529-024-03502-2","url":null,"abstract":"(+)-Ambrein is the primary component of ambergris, a rare product found in sperm whales (Physeter microcephalus). Microbial production using sustainable resources is a promising way to replace animal extraction and chemical synthesis. We constructed an engineered yeast strain to produce (+)-ambrein de novo. Squalene is a substrate for the biosynthesis of (+)-ambrein. Firstly, strain LQ2, with a squalene yield of 384.4 mg/L was obtained by optimizing the mevalonate pathway. Then we engineered a method for the de novo production of (+)-ambrein using glucose as a carbon source by overexpressing codon-optimized tetraprenyl-β-curcumene cyclase (BmeTC) and its double mutant enzyme (BmeTCY167A/D373C), evaluating different promoters, knocking out GAL80, and fusing the protein with BmeTC and squalene synthase (AtSQS2). Nevertheless, the synthesis of (+)-ambrein is still limited, causing low catalytic activity in BmeTC. We carried out a protein surface amino acid modification of BmeTC. The dominant mutant BmeTCK6A/Q9E/N454A for the first step was obtained to improve its catalytic activity. The yield of (+)-ambrein increased from 35.2 to 59.0 mg/L in the shake flask and finally reached 457.4 mg/L in the 2 L fermenter, the highest titer currently available for yeast. Efficiently engineered strains and inexpensive fermentation conditions for the industrial production of (+)-ambrein. The metabolic engineering tools provide directions for optimizing the biosynthesis of other high-value triterpenes.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141330356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxicants improve glycerol production in the fermentation of undetoxified hydrolysate by Candida glycerinogenes. 在甘油酸根念珠菌发酵未解毒水解物的过程中，毒物可提高甘油产量。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-07-31 DOI: 10.1007/s10529-024-03503-1

Xiaohong Zhao, Hong Zong, Xinyao Lu, Bin Zhuge

{"title":"Toxicants improve glycerol production in the fermentation of undetoxified hydrolysate by Candida glycerinogenes.","authors":"Xiaohong Zhao, Hong Zong, Xinyao Lu, Bin Zhuge","doi":"10.1007/s10529-024-03503-1","DOIUrl":"https://doi.org/10.1007/s10529-024-03503-1","url":null,"abstract":"Objectives: Toxicants inhibit microbial fermentation and reduce product titres. This work investigated the glycerol production characteristics of Candida glycerinogenes in highly toxic unwashed undetoxified hydrolysate and provided new ideas for high glycerol production from hydrolysates.Results: The unwashed hydrolysate contains higher concentrations of toxicants, such as furfural, acetic acid, phenols and NaCl than the washed alkali-treated bagasse hydrolysate. C. glycerinogenes fermented unwashed undetoxified hydrolysate yielded 36.1 g/L glycerol, 15.8% higher than the washed hydrolysate, suggesting that the toxicants stimulated glycerol synthesis. qRT-PCR analysis showed that toxicants of unwashed undetoxified hydrolysates greatly up-regulated the transcript levels of the genes GPD1, HXT4 and MSN4 et al. Overexpressing the above genes increased glycerol production by 27.9% to 46.1 g/L. And it was further increased by 8.8% to 50.1 g/L in a 5 L bioreactor.Conclusions: This result proves that toxicants in lignocellulosic hydrolysates can increase the titre of microbial glycerol production.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141858926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0