Biotechnology Letters最新文献_第4页

Essential amino acid residues and catalytic mechanism of trans-epoxysuccinate hydrolase for production of meso-tartaric acid. 反式环氧琥珀酸水解酶生产中酒石酸的必需氨基酸残基和催化机理。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-10-01 Epub Date: 2024-05-13 DOI: 10.1007/s10529-024-03490-3

Hongxiu Liao, Haifeng Pan, Jinfeng Yao, Ronglin Zhu, Wenna Bao

{"title":"Essential amino acid residues and catalytic mechanism of trans-epoxysuccinate hydrolase for production of meso-tartaric acid.","authors":"Hongxiu Liao, Haifeng Pan, Jinfeng Yao, Ronglin Zhu, Wenna Bao","doi":"10.1007/s10529-024-03490-3","DOIUrl":"10.1007/s10529-024-03490-3","url":null,"abstract":"Objectives: This study aimed to discuss the essential amino acid residues and catalytic mechanism of trans-epoxysuccinate hydrolase from Pseudomonas koreensis for the production of meso-tartaric acid.Results: The optimum conditions of the enzyme were 45 °C and pH 9.0, respectively. It was strongly inhibited by Zn2+, Mn2+ and SDS. Michaelis-Menten enzyme kinetics analysis gave a Km value of 3.50 mM and a kcat of 99.75 s-1, with an exceptional EE value exceeding 99.9%. Multiple sequence alignment and homology modeling revealed that the enzyme belonged to MhpC superfamily and possessed a typical α/β hydrolase folding structure. Site-directed mutagenesis indicated H34, D104, R105, R108, D128, Y147, H149, W150, Y211, and H272 were important catalytic residues. The 18O-labeling study suggested the enzyme acted via two-step catalytic mechanism.Conclusions: The structure and catalytic mechanism of trans-epoxysuccinate hydrolase were first reported. Ten residues were critical for its catalysis and a two-step mechanism by an Asp-His-Asp catalytic triad was proposed.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"739-749"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140916047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From yeast screening for suitability as single cell protein to fed-batch cultures. 从筛选适合作为单细胞蛋白质的酵母到饲料批量培养。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-10-01 Epub Date: 2024-07-13 DOI: 10.1007/s10529-024-03504-0

Alexander Anderson, Adriaan Van der Mijnsbrugge, Xavier Cameleyre, Nathalie Gorret

{"title":"From yeast screening for suitability as single cell protein to fed-batch cultures.","authors":"Alexander Anderson, Adriaan Van der Mijnsbrugge, Xavier Cameleyre, Nathalie Gorret","doi":"10.1007/s10529-024-03504-0","DOIUrl":"10.1007/s10529-024-03504-0","url":null,"abstract":"Purpose: Fed-batch cultures have rarely been used in single cell protein (SCP) research. This work evaluated multiple yeast species for suitability as SCP cultivated using glucose- and sucrose-based substrate and performed in-depth studies of fed-batch SCP cultivation kinetics for selected yeasts, including determination of specific crude nitrogen-to-protein conversion factors.Methods: SCP was cultivated using fully synthetic media in flask batch or bioreactor fed-batch cultures. Crude nitrogen and nucleic acid content were determined using the Dumas method and fluorescence assay kits, respectively.Results: C. utilis compared favorably to other yeasts in flask batch cultures in terms of process yield (0.52 ± 0.01 gx gs-1) and crude nitrogen content (10.0 ± 0.5 and 9.9 ± 0.5%CDW for glucose and sucrose, respectively). This is the first time biomass composition data was reported for SCP cultivated in fed-batch mode. C. utilis crude nitrogen content was consistent across the tested conditions (protein content stabilized around 50%CDW in fed-batch), while that of the benchmark yeast S. cerevisiae was higher in batch cultures and at the beginning of fed-batch relative to the end (protein content decreased over time and stabilized around 43%CDW). Total nucleic acid content of the yeasts was similar (6.8%CDW and 6.3%CDW, for C. utilis and S. cerevisiae, respectively), with crude nitrogen-to-protein conversion factors of 4.97 and 5.80.Conclusion: This study demonstrated the suitability of C. utilis as SCP, notably the robustness of its crude nitrogen content (as an indicator of protein content) across batch and fed-batch conditions, compared to that of the benchmark yeast S. cerevisiae.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"827-842"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141603271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of a pearl millet WRKY transcription factor gene, PgWRKY74, in Arabidopsis retards shoot growth under dehydration and salinity-stressed conditions. 拟南芥中珍珠粟 WRKY 转录因子基因 PgWRKY74 的过表达可延缓脱水和盐分胁迫条件下的幼芽生长。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-10-01 Epub Date: 2024-05-08 DOI: 10.1007/s10529-024-03492-1

Maimuna Qazi, Shashi Kumar Gupta, Tetsuo Takano, Daisuke Tsugama

{"title":"Overexpression of a pearl millet WRKY transcription factor gene, PgWRKY74, in Arabidopsis retards shoot growth under dehydration and salinity-stressed conditions.","authors":"Maimuna Qazi, Shashi Kumar Gupta, Tetsuo Takano, Daisuke Tsugama","doi":"10.1007/s10529-024-03492-1","DOIUrl":"10.1007/s10529-024-03492-1","url":null,"abstract":"Pearl millet (Cenchrus americanus) is a cereal crop that can tolerate high temperatures, drought, and low-fertility conditions where other crops lose productivity. However, genes regulating this ability are largely unknown. Transcription factors (TFs) regulate transcription of their target genes, regulate downstream biological processes, and thus are candidates for regulators of such tolerance of pearl millet. PgWRKY74 encodes a group IIc WRKY TF in pearl millet and is downregulated by drought. PgWRKY74 may have a role in drought tolerance. The objective of this study was to gain insights into the physiological and biochemical functions of PgWRKY74. Yeast one-hybrid and gel shift assays were performed to examine transcriptional activation potential and deoxyribonucleic acid (DNA)-binding ability, respectively. Transgenic Arabidopsis thaliana plants overexpressing PgWRKY74-green fluorescent protein (GFP) fusion gene were generated and tested for growth and stress-responsive gene expression under mannitol and NaCl-stressed conditions. A construct with PgWRKY74 enabled yeast reporter cells to survive on test media in the yeast one-hybrid assays. The electrophoretic mobility of DNA with putative WRKY TF-binding motifs was lower in the presence of a recombinant PgWRKY74 protein than its absence. The PgWRKY74-GFP-overexpressing Arabidopsis plants exhibited smaller rosette areas than did wild-type plants under mannitol-stressed and NaCl-stressed conditions, and exhibited weaker expression of RD29B, which is induced by the stress-related phytohormone abscisic acid (ABA), under the mannitol-stressed condition. PgWRKY74 have transcriptional activation potential and DNA-binding ability, and can negatively regulate plant responses to mannitol and NaCl stresses, possibly by decreasing ABA levels or ABA sensitivity.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"851-860"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415432/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140875753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocontrol of strawberry Botrytis gray mold and prolong the fruit shelf-life by fumigant Trichoderma spp. 用熏蒸剂毛霉菌属生物防治草莓灰霉病并延长水果货架期

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-10-01 Epub Date: 2024-05-29 DOI: 10.1007/s10529-024-03498-9

Q S Fan, H J Lin, Y J Hu, J Jin, H H Yan, R Q Zhang

{"title":"Biocontrol of strawberry Botrytis gray mold and prolong the fruit shelf-life by fumigant Trichoderma spp.","authors":"Q S Fan, H J Lin, Y J Hu, J Jin, H H Yan, R Q Zhang","doi":"10.1007/s10529-024-03498-9","DOIUrl":"10.1007/s10529-024-03498-9","url":null,"abstract":"Objectives To screen high active volatile organic compounds (VOCs)-producing Trichoderma isolates against strawberry gray mold caused by Botrytis cinerea, and to explore their antagonistic mode of action against the pathogen. VOCs produced by nine Trichoderma isolates (Trichoderma atroviride T1 and T3; Trichoderma harzianum T2, T4 and T5; T6, T7, T8 and T9 identified as Trichoderma asperellum in this work) significantly inhibited the mycelial growth (13.9-63.0% reduction) and conidial germination (17.6-96.3% reduction) of B. cinerea, the highest inhibition percentage belonged to VOCs of T7; in a closed space, VOCs of T7 shared 76.9% and 100% biocontrol efficacy against gray mold on strawberry fruits and detached leaves, respectively, prolonged the fruit shelf-life by 3 days in presence of B. cinerea, completely protected the leaves from B. cinerea infecting; volatile metabolites of T7 damaged the cell membrane permeability and integrity of B. cinerea, thereby inhibiting the mycelial growth and conidial germination. Gas chromatography-mass spectrometry (GC-MS) analysis revealed the VOCs contain 23 potential compounds, and the majority of these compounds were categorised as alkenes, alcohols, and esters, including PEA and 6PP, which have been reported as substances produced by Trichoderma spp. T. asperellum T7 showed high biofumigant activity against mycelial growth especially conidial germination of B. cinerea and thus protected strawberry fruits and leaves from gray mold, which acted by damaging the pathogen's plasma membrane and resulting in cytoplasm leakage, was a potential biofumigant for controlling pre- and post-harvest strawberry gray mold.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"751-766"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141174405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a bacterial cellulose-gelatin composite as a suitable scaffold for cardiac tissue engineering. 开发细菌纤维素-明胶复合材料，作为心脏组织工程的合适支架。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-10-01 Epub Date: 2024-05-21 DOI: 10.1007/s10529-024-03477-0

Mohaddeseh Salehghamari, Mansour Mashreghi, Maryam M Matin, Zeinab Neshati

{"title":"Development of a bacterial cellulose-gelatin composite as a suitable scaffold for cardiac tissue engineering.","authors":"Mohaddeseh Salehghamari, Mansour Mashreghi, Maryam M Matin, Zeinab Neshati","doi":"10.1007/s10529-024-03477-0","DOIUrl":"10.1007/s10529-024-03477-0","url":null,"abstract":"Purpose: Cardiac tissue engineering is suggested as a promising approach to overcome problems associated with impaired myocardium. This is the first study to investigate the use of BC and gelatin for cardiomyocyte adhesion and growth.Methods: Bacterial cellulose (BC) membranes were produced by Komagataeibacter xylinus and coated or mixed with gelatin to make gelatin-coated BC (BCG) or gelatin-mixed BC (mBCG) scaffolds, respectively. BC based-scaffolds were characterized via SEM, FTIR, XRD, and AFM. Neonatal rat-ventricular cardiomyocytes (nr-vCMCs) were cultured on the scaffolds to check the capability of the composites for cardiomyocyte attachment, growth and expansion.Results: The average nanofibrils diameter in all scaffolds was suitable (~ 30-65 nm) for nr-vCMCs culture. Pore diameter (≥ 10 µm), surface roughness (~ 182 nm), elastic modulus (0.075 ± 0.015 MPa) in mBCG were in accordance with cardiomyocyte requirements, so that mBCG could better support attachment of nr-vCMCs with high concentration of gelatin, and appropriate surface roughness. Also, it could better support growth and expansion of nr-vCMCs due to submicron scale of nanofibrils and proper elasticity (~ 0.075 MPa). The viability of nr-vCMCs on BC and BCG scaffolds was very low even at day 2 of culture (~ ≤ 40%), but, mBCG could promote a metabolic active state of nr-vCMCs until day 7 (~ ≥ 50%).Conclusion: According to our results, mBCG scaffold was the most suitable composite for cardiomyocyte culture, regarding its physicochemical and cell characteristics. It is suggested that improvement in mBCG stability and cell attachment features may provide a convenient scaffold for cardiac tissue engineering.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"887-905"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesenchymal stem cell-derived cell-free technologies: a patent landscape. 间充质干细胞衍生的无细胞技术：专利概况。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-10-01 Epub Date: 2024-06-20 DOI: 10.1007/s10529-024-03506-y

Luiza Carolina França Opretzka, Cláudio Damasceno Pinto, Jânio Rodrigo de Jesus Santos, Alyne Almeida de Lima, Milena Botelho Pereira Soares, Cristiane Flora Villarreal

引用次数: 0

Nepeta cataria L. (catnip) can serve as a chassis for the engineering of secondary metabolic pathways. 猫薄荷（Nepeta cataria L.）可以作为次级代谢途径工程的底盘。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-10-01 Epub Date: 2024-05-08 DOI: 10.1007/s10529-024-03489-w

Marcus Geissler, Christoph Neubauer, Yuriy V Sheludko, Adrian Brückner, Heribert Warzecha

{"title":"Nepeta cataria L. (catnip) can serve as a chassis for the engineering of secondary metabolic pathways.","authors":"Marcus Geissler, Christoph Neubauer, Yuriy V Sheludko, Adrian Brückner, Heribert Warzecha","doi":"10.1007/s10529-024-03489-w","DOIUrl":"10.1007/s10529-024-03489-w","url":null,"abstract":"Objective: Evaluation of Nepeta cataria as a host with specific endogenous metabolite background for transient expression and metabolic engineering of secondary biosynthetic sequences.Results: The reporter gene gfp::licBM3 as well as three biosynthetic genes leading to the formation of the cannabinoid precursor olivetolic acid were adopted to the modular cloning standard GoldenBraid, transiently expressed in two chemotypes of N. cataria and compared to Nicotiana benthamiana. To estimate the expression efficiency in both hosts, quantification of the reporter activity was carried out with a sensitive and specific lichenase assay. While N. benthamiana exhibited lichenase activity of 676 ± 94 μmol g-1 s-1, N. cataria cultivar '1000', and the cultivar 'Citriodora' showed an activity of 37 ± 8 μmol g-1 s-1 and 18 ± 4 μmol g-1 s-1, respectively. Further, combinatorial expression of genes involved in cannabinoid biosynthetic pathway acyl-activating enzyme 1 (aae1), olivetol synthase (ols) and olivetolic acid cyclase (oac) in N. cataria cv. resulted presumably in the in vivo production of olivetolic acid glycosides.Conclusion: Nepeta cataria is amenable to Agrobacterium-mediated transient expression and could serve as a novel chassis for the engineering of secondary metabolic pathways and transient evaluation of heterologous genes.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"843-850"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140875752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of L-cysteine on starch and protein degradation during barley germination. L-半胱氨酸对大麦发芽过程中淀粉和蛋白质降解的影响

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-10-01 Epub Date: 2024-06-25 DOI: 10.1007/s10529-024-03508-w

Shumin Hu, Qingqing Qin, Cui Zhang, Junhong Yu, Shuli Huang, Jia Liu, Zhaoxia Yang

{"title":"The effect of L-cysteine on starch and protein degradation during barley germination.","authors":"Shumin Hu, Qingqing Qin, Cui Zhang, Junhong Yu, Shuli Huang, Jia Liu, Zhaoxia Yang","doi":"10.1007/s10529-024-03508-w","DOIUrl":"10.1007/s10529-024-03508-w","url":null,"abstract":"Objectives: In order to investigate the impact of L-cysteine (L-Cys) on starch and protein degradation during barley germination. The amylase activities, degradation of macromolecules during germination were determined in this study.Methods: Barley was germinated in petri dish for 0 to 5 days with different levels of L-Cys (0 mM, 2.5 mM, 5 mM, 10 mM).Results: L-Cys addition increased the total limit dextrinase (LD) activities and decreased the LD inhibitor activities during whole germination stage. The activities of α-amylase, β-amylase and free LD were increased with the addition of 2.5, 5 mM L-Cys at germination days 1 to 4. Due to higher amylase in malt with the addition of L-Cys, the non-fermentable sugars were reduced and the glucose, maltotriose were improved. Furthermore, the protein degradation analysis showed that low molecular weight protein increased and middle molecular weight protein decreased obviously in wort from the malt germinated with L-Cys, demonstrating that the L-Cys promote the protein degradation. Lastly, the filtration performance of malt with the addition of L-Cys during malting was better than the control.Conclusion: In conclusion, L-Cys can promote the degradation of storage material (starch, protein) during barley germination, leading to a better green malt quality.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"861-870"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141445355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrile hydratase as a promising biocatalyst: recent advances and future prospects 腈水解酶作为一种前景广阔的生物催化剂：最新进展与未来展望

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-09-13 DOI: 10.1007/s10529-024-03530-y

Chao Feng, Jing Chen, Wenxin Ye, Zhanshi Wang

{"title":"Nitrile hydratase as a promising biocatalyst: recent advances and future prospects","authors":"Chao Feng, Jing Chen, Wenxin Ye, Zhanshi Wang","doi":"10.1007/s10529-024-03530-y","DOIUrl":"https://doi.org/10.1007/s10529-024-03530-y","url":null,"abstract":"Amides are an important type of synthetic intermediate used in the chemical, agrochemical, pharmaceutical, and nutraceutical industries. The traditional chemical process of converting nitriles into the corresponding amides is feasible but is restricted because of the harsh conditions required. In recent decades, nitrile hydratase (NHase, EC 4.2.1.84) has attracted considerable attention because of its application in nitrile transformation as a prominent biocatalyst. In this review, we provide a comprehensive survey of recent advances in NHase research in terms of natural distribution, enzyme screening, and molecular modification on the basis of its characteristics and catalytic mechanism. Additionally, industrial applications and recent significant biotechnology advances in NHase bioengineering and immobilization techniques are systematically summarized. Moreover, the current challenges and future perspectives for its further development in industrial applications for green chemistry were also discussed. This study contributes to the current state-of-the-art, providing important technical information for new NHase applications in manufacturing industries.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"25 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immobilization coupling with aptamer assisted dual cycle amplification for sensitive sEVs isolation and analysis 用于灵敏 sEVs 分离和分析的固定耦合与适配体辅助双循环扩增技术

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-09-12 DOI: 10.1007/s10529-024-03526-8

Yu He, Ying Ren, Jiawen Tang

{"title":"Immobilization coupling with aptamer assisted dual cycle amplification for sensitive sEVs isolation and analysis","authors":"Yu He, Ying Ren, Jiawen Tang","doi":"10.1007/s10529-024-03526-8","DOIUrl":"https://doi.org/10.1007/s10529-024-03526-8","url":null,"abstract":"Precise identification of small extracellular vesicles (sEVs) is crucial for improving disease diagnosis and treatments, such as bladder cancer. However, accurate isolation and simultaneously quantification of sEVs remain a huge challenge. We have introduced a new technique that combines immobilization with aptamer-assisted dual cycle amplification to isolate and analyze sEVs with high sensitivity. In this method, the CD9 protein antibody is attached to the plate’s surface for the initial identification of sEVs, while an aptamer probe is used to detect the exosomal surface protein CD63. We have created an sEVs-surface method that combines target recognition initiated signal recycling and rolling circle amplification (RCA) for signal amplification. This approach allows for the “AND” logic analysis of dual biomarkers, enabling both sEVs quantification and tracing. The proposed approach has a broad detection range and a low limit of detection. Moreover, the established method showed good stability in detecting sEVs with a low coefficient of variation. Our method can effectively isolate certain sEVs and accurately identify them, making it suitable for many uses in biological science, biomedical engineering, and personalized medicine.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"5 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0