Biotechnology Letters最新文献_第4页

Genetic engineering of Beauveria bassiana for enhancing siderophore production and its virulence against the insect hosts. 球孢白僵菌提高铁载体产量及其对昆虫宿主毒力的基因工程研究。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-02-14 DOI: 10.1007/s10529-026-03706-8

Qian-Xuan Yu, Ting-Fei Sun, Ming-Guang Feng, Sheng-Hua Ying

{"title":"Genetic engineering of Beauveria bassiana for enhancing siderophore production and its virulence against the insect hosts.","authors":"Qian-Xuan Yu, Ting-Fei Sun, Ming-Guang Feng, Sheng-Hua Ying","doi":"10.1007/s10529-026-03706-8","DOIUrl":"10.1007/s10529-026-03706-8","url":null,"abstract":"This study is designed to demonstrate the divergent siderophore production among the strains of the entomopathogenic fungus Beauveria bassiana and enhance the siderophore production by overexpression of the biosynthesis-related genes. Chrome azurol S (CAS) assay indicated B. bassiana strains displayed divergent abilities to produce siderophores under the iron-limited conditions. A strain (Bb3153) did not produce enough siderophores detected by CAS assay and was considered as a non-siderophore-producing strain. Comparative transcriptomic analysis was conducted between the strains Bb3153 and Bb2860 (a siderophore-producing strain). The results indicated the significant difference in global expression between these two strains, in which the siderophore synthesis gene sidL, encoding N5-hydroxyornithine acetylase, were significantly repressed in the strain Bb3153 when compared with the strain Bb2860. A recombinant strain (Bb3153OE) was constructed by overexpressing sidL in Bb3153. CAS assay indicated that Bb3153OE produced siderophores ahead of its parent strain. Mass spectrometry analysis indicated that the production of dimerumic acid was significantly increased. Phenotypic assays indicated the recombinant strain displayed the enhanced growth and virulence. This study highlights the genetic plasticity of siderophore biosynthesis in the filamentous entomopathogenic fungi, which serves as a promising strategy to enhance their biocontrol efficacy.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 2","pages":"37"},"PeriodicalIF":2.1,"publicationDate":"2026-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146194040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioinformatic evaluation of ClpP as a vaccine target in Streptococcus pneumoniae. ClpP作为肺炎链球菌疫苗靶点的生物信息学评价。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-02-11 DOI: 10.1007/s10529-026-03697-6

Yanying Huang, Liyan Zhu, Weihua Li, Shilei Dong

{"title":"Bioinformatic evaluation of ClpP as a vaccine target in Streptococcus pneumoniae.","authors":"Yanying Huang, Liyan Zhu, Weihua Li, Shilei Dong","doi":"10.1007/s10529-026-03697-6","DOIUrl":"10.1007/s10529-026-03697-6","url":null,"abstract":"Purpose: To conduct a comprehensive bioinformatic analysis to assess the immunogenicity and conservation profiles of ClpP in Streptococcus pneumoniae, thereby verifying its feasibility as a candidate vaccine target.Methods: The nucleotide and amino acid sequences of the clpP gene were obtained from the NCBI database. The fundamental characterizations of ClpP were retrieved from the public database PneumoWiki. NetPhos was used to predict phosphorylation sites, whereas SOPMA, Swiss-Model and STRING were employed to analyze secondary/tertiary structures and functional interaction network, respectively. Antigenic epitope mapping was carried out using DNASTAR, SYFPEITHI, NetMHCIIpan 4.0 and NetMHC. Multiple sequence alignment (MSA) was performed using Jalview software to analyze the conservation of the predicted epitopes across S. pneumoniae strains. Concurrently, a phylogenetic tree was constructed to evaluate the cross-species conservation of the ClpP protein.Results: ClpP (196 aa, pI 4.74) showed high hydrophobicity and without signal peptides/transmembrane domains. Phosphorylation sites (Ser/Thr/Tyr) were identified. The secondary structure of ClpP consists of 94 α-helices, 37 β-strands and 65 random coils. The functional interaction network of ClpP involves 10 interacting partners. Immunoinformatic analysis further detected immunodominant B-cell and T-cell epitopes within the ClpP sequence.Conclusion: Taken together, these data suggest that ClpP exhibits robust immunogenicity and multiple antigenic epitopes, supporting its potential as a peptide-based vaccine candidate against S. pneumoniae.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 2","pages":"36"},"PeriodicalIF":2.1,"publicationDate":"2026-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146155932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-quorum, anti biofilm activity of FDA approved drugs against P. aeruginosa: in silico and in vitro studies. FDA批准的抗铜绿假单胞菌药物的抗聚集、抗生物膜活性：计算机和体外研究。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-02-10 DOI: 10.1007/s10529-026-03693-w

Vivek Kumar, Anmol Srivastava, Vishnu Agarwal

{"title":"Anti-quorum, anti biofilm activity of FDA approved drugs against P. aeruginosa: in silico and in vitro studies.","authors":"Vivek Kumar, Anmol Srivastava, Vishnu Agarwal","doi":"10.1007/s10529-026-03693-w","DOIUrl":"10.1007/s10529-026-03693-w","url":null,"abstract":"P. aeruginosa is an opportunistic pathogen that causes various nosocomial infections. The ability of P. aeruginosa to form biofilms is one of the main factors contributing to its pathogenicity. Due to biofilm formation, bacteria get embedded in it and is able to withstand extreme environmental conditions like chemicals, UV, temperature, pH, salinity, and antibiotics. Biofilm formation is an important virulence factor associated with quorum sensing (QS), which is a cell-to-cell communication system that is influenced by cell density. P. aeruginosa is a notorious pathogen that is known to cause severe complications in patients suffering from cystic fibrosis and immuno-compromised individuals in hospital setting as a result of biofilm formation. We might limit P. aeruginosa infection pathogenesis and biofilm formation if we can disrupt the signalling molecules involved in QS. In this study, we suggested that already-approved medications by the FDA could be employed as anti-quorum and anti-biofilm agents and potentially be helpful in curing P. aeruginosa associated infections. The anti-quorum and anti-biofilm properties of FDA-approved medications have been investigated here. We have also performed RT-PCR analysis and molecular docking experiments to assess the mechanism of action of these drugs. We have found that all four drugs have anti QS activity. Out of these four drugs Flurbiprofen was found to be more effective. RT data confirms that these drugs have significantly downregulated all four QS gene and therefore are able to inhibit the P. aeruginosa virulence factors. This study significantly opens up the new horizons for the development of novel therapeutics against P. aeruginosa and infections associated with it.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 2","pages":"35"},"PeriodicalIF":2.1,"publicationDate":"2026-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146148857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the effects of fish protein hydrolysates on Pseudomonas aeruginosa growth and exotoxin A expression. 评价鱼蛋白水解物对铜绿假单胞菌生长和外毒素A表达的影响。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-02-10 DOI: 10.1007/s10529-026-03708-6

Zahra Yaghoubzadeh, Maryam Ghiasi

{"title":"Evaluating the effects of fish protein hydrolysates on Pseudomonas aeruginosa growth and exotoxin A expression.","authors":"Zahra Yaghoubzadeh, Maryam Ghiasi","doi":"10.1007/s10529-026-03708-6","DOIUrl":"10.1007/s10529-026-03708-6","url":null,"abstract":"The present study aimed to valorize rainbow trout (Oncorhynchus mykiss) processing waste through enzymatic hydrolysis using Alcalase and Flavourzyme, to produce protein hydrolysates with enhanced biofunctional properties. Hydrolysates were evaluated for their chemical composition, antimicrobial activity, antioxidant capacity, and molecular effects on Pseudomonas aeruginosa. Biochemical analysis revealed that enzymatic treatment significantly increased protein content and reduced moisture levels compared to crude extracts. Antimicrobial assays showed that while crude proteins exhibited no inhibitory effect, hydrolysates particularly those derived from Alcalase demonstrated clear inhibition zones against P. aeruginosa. Quantitative PCR confirmed microbial suppression by showing delayed amplification curves in treated samples. Antioxidant activity, measured via IC₅₀ values, indicated that Alcalase-generated hydrolysates possessed superior radical-scavenging capacity. The observed bioactivity was closely linked to peptide size distribution and enzyme specificity. These findings suggest that rainbow trout waste hydrolysates are promising candidates for use as natural antimicrobial and antioxidant agents in food, pharmaceutical, and environmental applications. Further studies are recommended to isolate and characterize the active peptides and assess their efficacy in real-world systems.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 2","pages":"34"},"PeriodicalIF":2.1,"publicationDate":"2026-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146148865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene expression profiling to elucidate the promotive effects of the volatile organic compound 3-octanone on the mycelial growth of Ganoderma lucidum. 利用基因表达谱研究挥发性有机化合物3-辛酮对灵芝菌丝生长的促进作用。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-02-05 DOI: 10.1007/s10529-026-03698-5

Shoko Horikawa, Ryuka Iizuka, Kiwamu Umezawa, Rumi Konuma, Makoto Yoshida

{"title":"Gene expression profiling to elucidate the promotive effects of the volatile organic compound 3-octanone on the mycelial growth of Ganoderma lucidum.","authors":"Shoko Horikawa, Ryuka Iizuka, Kiwamu Umezawa, Rumi Konuma, Makoto Yoshida","doi":"10.1007/s10529-026-03698-5","DOIUrl":"10.1007/s10529-026-03698-5","url":null,"abstract":"To investigate the effects of fungal volatile organic compounds (FVOCs) on the mycelial growth of Ganoderma lucidum, and to elucidate the molecular mechanisms underlying the growth-promoting effect of 3-octanone. G. lucidum was cultivated with 1-octen-3-ol, 3-octanol and 3-octanone for 7 days, after which colony diameter and mycelial dry weight were measured to assess their effects on mycelial growth. RNA-seq was used to investigate gene expression changes following 3-octanone exposure. While 1-octen-3-ol or 3-octanol inhibited mycelial growth in G. lucidum, 3-octanone promoted it. In total, 590 differentially expressed genes (DEGs), including 162 upregulated and 428 downregulated genes, were identified following 3-octanone exposure. Functional annotation revealed that among the DEGs, 23 genes were related to fungal cell wall biosynthesis and remodeling, whereas 21 genes were involved in plant-derived polysaccharide degradation. Furthermore, significant expression changes were observed in genes related to secondary metabolism. Our results indicate that G. lucidum can use 3-octanone as a signal to recognize other fungi, potentially facilitating the expansion of its own territory within wood in nature.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 1","pages":"32"},"PeriodicalIF":2.1,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12876093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146123480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of cultivation performance of S. cerevisiae strains expressing GLP-1-GIP peptide precursors. 表达GLP-1-GIP肽前体的酿酒葡萄球菌培养性能评价。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-02-05 DOI: 10.1007/s10529-026-03704-w

Ioannis Voulgaris, Anders Nygaard Nielsen, Tine Petersen, Jakob Brandt, Sanne Jensen

引用次数: 0

Cellulose microfiber production from green seaweed Ulva lactuca using hydrated deep eutectic solvent. 利用水合深共熔溶剂制备绿海藻纤维素超细纤维。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-02-05 DOI: 10.1007/s10529-026-03702-y

Rizfi Fariz Pari, Safrina Dyah Hardiningtyas, Wahyu Ramadhan, Uju, Rie Wakabayashi, Masahiro Goto, Noriho Kamiya

{"title":"Cellulose microfiber production from green seaweed Ulva lactuca using hydrated deep eutectic solvent.","authors":"Rizfi Fariz Pari, Safrina Dyah Hardiningtyas, Wahyu Ramadhan, Uju, Rie Wakabayashi, Masahiro Goto, Noriho Kamiya","doi":"10.1007/s10529-026-03702-y","DOIUrl":"10.1007/s10529-026-03702-y","url":null,"abstract":"We investigated the effectiveness of hydrated deep eutectic solvents (DESs) to achieve tunable seaweed cellulose microstructures from Ulva lactuca. Cellulose was extracted via a sequential chemical protocol, then treated with 30% DESs (choline chloride (ChCl) or betaine as hydrogen bond acceptors and urea, citric acid, or oxalic acid as donors) combined with mechanical shearing. While most DESs combinations yielded spherical seaweed cellulose microparticles (dry diameter of 605-777 nm), the ChCl:urea formulation successfully produced seaweed cellulose microfibers (SCMFs). These SCMFs exhibited superior quality (dry diameter of 372 nm), and water dispersibility with a hydrodynamic diameter of 134 nm and a polydispersity index of 0.23. Crucially, the DES composition dictated cellulose structure: ChCl:urea-treated SCMF remained predominantly amorphous, whereas other DESs treatments increased microparticles crystallinity. Furthermore, ChCl:oxalic acid introduced carboxyl functional groups. Selecting appropriate hydrated DESs thus offers a sustainable biotechnology tool to tune cellulose morphology, crystallinity, and surface chemistry.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 1","pages":"30"},"PeriodicalIF":2.1,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146123525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable biodegradation of phenol using peroxidases extracted from local agricultural wastes. 利用从当地农业废弃物中提取的过氧化物酶实现苯酚的可持续生物降解。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-02-05 DOI: 10.1007/s10529-026-03700-0

Tung Chiong, Sie Yon Lau, Michael K Danquah, Murat Yılmaz

{"title":"Sustainable biodegradation of phenol using peroxidases extracted from local agricultural wastes.","authors":"Tung Chiong, Sie Yon Lau, Michael K Danquah, Murat Yılmaz","doi":"10.1007/s10529-026-03700-0","DOIUrl":"10.1007/s10529-026-03700-0","url":null,"abstract":"The present study focused on the enhanced biodegradation of phenol as a toxic compound using peroxidases extracted from agricultural wastes (skin peels of luffa and jicama plants) mediated bioprocess under H2O2-infusion. The extracted crude luffa peroxidase (LP) and jicama peroxidase (JP) showed enzyme activities of 1.4 ± 0.2 U/mL and 1.6 ± 0.1 U/mL, respectively. LP achieved a phenol removal efficiency exceeding 95% at H₂O₂ concentrations between 6-8 mM and pH 7, whereas JP achieved 94.5% removal at pH 7, with minimal impact from variations in H₂O₂ concentrations. Maximum removal efficiencies for both peroxidases were observed at an enzyme loading of 1.5 mL. LP demonstrated effective removal within a temperature range of 25 °C to 30 °C, while JP maintained functionality up to 40 °C. The optimum reaction times were determined to be 16 h for LP and 13 h for JP. LP exhibited high preservation of enzyme activity during storage, with no significant fluctuations observed, while JP's enzyme activity decreased to approximately 40% of its initial value, accompanied by a notable decline in phenol elimination efficiency by day 42. First-order kinetic analysis of enzyme activity yielded reaction rate constants of 1.18 h-1 for LP and 1.21 h-1 for JP, with JP demonstrating superior storage stability compared to LP.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 1","pages":"29"},"PeriodicalIF":2.1,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146123489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing Lactiplantibacillus plantarum JCM 1149 growth conditions for improvement of the gelation and nutritional qualities of fermented soy milk. 优化植物乳杆菌JCM 1149生长条件，提高发酵豆浆的凝胶性和营养品质。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-02-05 DOI: 10.1007/s10529-026-03701-z

Binta Buba Adamu, Jeremiah David Bala, Helen Shnada Auta, Ocheme Ocheme Boniface, Oluwafemi Adebayo Oyewole, Hafsat Babayi, Mercy Itohan Aboh, Naga Raju Maddela, Ram Prasad

{"title":"Optimizing Lactiplantibacillus plantarum JCM 1149 growth conditions for improvement of the gelation and nutritional qualities of fermented soy milk.","authors":"Binta Buba Adamu, Jeremiah David Bala, Helen Shnada Auta, Ocheme Ocheme Boniface, Oluwafemi Adebayo Oyewole, Hafsat Babayi, Mercy Itohan Aboh, Naga Raju Maddela, Ram Prasad","doi":"10.1007/s10529-026-03701-z","DOIUrl":"10.1007/s10529-026-03701-z","url":null,"abstract":"Exopolysaccharide (EPS) is a biopolymer produced by microorganisms. This study aimed to optimize EPS production from lactic acid bacteria (LAB) to enhance the gelation quality of fermented soy milk by varying physical growth conditions. EPS was purified using gel filtration column chromatography, and the proximate composition of the fermented soy milk was analyzed according to AOAC methods. Lactiplantibacillus plantarum JCM 1149 produced a crude EPS yield of 166.4 mg/100 mL under optimized conditions (i.e. 35 °C, pH 4.8, 0.5 mL inoculum) after 24 h, while the purified EPS was 158.6 mg/100 mL with a glucose equivalent of 0.209 g/100 mL. Fermented soy milk produced under these conditions (sample T) was compared to a control prepared with a commercial yogurt starter culture (sample C). Sample T had a higher water-holding capacity (69.1%) and lower syneresis (35.80%) than sample C (30.80% and 41.01%, respectively). No significant differences (p > 0.05) were observed between samples for protein, lipid, nitrogen-free extract, or fiber content. However, sample T showed significantly higher moisture content (80.62 ± 0.30%) and energy (46.64 ± 0.29 kcal) slightly lower ash content (0.37 ± 0.00%) compared to sample C (0.41 ± 0.00%). Total viable counts were similar between samples C (7.10 × 102 cfu/mL) and T (7.60 × 102 cfu/mL). Sensory evaluation indicated that sample T had higher overall acceptability (7.46 ± 0.08) than sample C (6.98 ± 0.10). These results demonstrate that EPS-producing LAB can improve the gelation, functional, and nutritional qualities of fermented soy milk.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 1","pages":"28"},"PeriodicalIF":2.1,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146123497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of three light-dark cycles on circadian rhythm-related genes and photosynthetic parameters in carrot. 三个光暗循环对胡萝卜昼夜节律相关基因和光合参数的影响。

IF 2.1 4区生物学

Biotechnology Letters Pub Date : 2026-02-05 DOI: 10.1007/s10529-026-03703-x

Nan Zhang, Zhi-Hang Hu, Li-Xiang Wang, Chen Chen, Ai-Sheng Xiong

{"title":"Effects of three light-dark cycles on circadian rhythm-related genes and photosynthetic parameters in carrot.","authors":"Nan Zhang, Zhi-Hang Hu, Li-Xiang Wang, Chen Chen, Ai-Sheng Xiong","doi":"10.1007/s10529-026-03703-x","DOIUrl":"10.1007/s10529-026-03703-x","url":null,"abstract":"Carrot (Daucus carota L.), a key crop in the Apiaceae family, adapts to environmental changes through its endogenous circadian clock, which coordinates physiological processes over a 24-h cycle. As a central physiological activity of carrot growth, photosynthesis, including parameters such as photosynthetic efficiency and stomatal conductance, is influenced by environmental factors. Among them, photoperiod is one of the main regulatory factors for development and chlorophyll synthesis. In this study, we investigated the effects of three different light cycles (16 h of light/8 h of darkness, 16L/8D, 12 h of light/12 h of darkness, 12L/12D, and 8 h of light/16 h of darkness, 8L/16D) on the expression of circadian rhythm genes in carrot. The core clock genes, DcLNK1, DcLNK3, DcRVEa, DcRVEb, and DcGI exhibited distinct transcriptional responses to these light cycles. Three different photoperiod treatments led to changes in the expression profiles of circadian rhythm related genes, photosynthetic parameters, and stomatal activity in carrot. The results elucidated a potential molecular regulatory network linking photoperiod perception to physiological pathway in carrot, providing potential insight into the intrinsic mechanisms of photo-entrainment within its circadian system.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 1","pages":"31"},"PeriodicalIF":2.1,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146123499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0