Biotechnology Letters最新文献_第5页

Paddy straw saccharification using immobilized laccase on magnetized multiwall carbon nanotubes. 利用磁化多壁碳纳米管上的固定化漆酶对稻草进行糖化。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-05-15 DOI: 10.1007/s10529-024-03494-z

Hasnol Azahari Natasha Yasmin, Balakrishnan Kunasundari, Siew Hoong Shuit, Mohamad Fahrurrazi Tompang

{"title":"Paddy straw saccharification using immobilized laccase on magnetized multiwall carbon nanotubes.","authors":"Hasnol Azahari Natasha Yasmin, Balakrishnan Kunasundari, Siew Hoong Shuit, Mohamad Fahrurrazi Tompang","doi":"10.1007/s10529-024-03494-z","DOIUrl":"10.1007/s10529-024-03494-z","url":null,"abstract":"The effective recovery of the immobilized enzymes using magnetic carriers has led to growing interest in this technology. The objective of this research was to evaluate the efficiency of immobilized laccase on magnetized multiwall carbon nanotubes (m-MWCNTs) in terms of stability and reusability. Laccases were efficiently adsorbed onto magnetized multiwall carbon nanotubes (m-MWCNTs) synthesized using water. The concentration of 7 mg laccase/mL was found to be ideal for immobilization. The optimal activity of both free and immobilized laccases was observed at pH 5, while for the latter, the optimal temperature was shifted from 40 to 50 °C. Compared to the free laccase, the immobilized laccase exhibited a greater range of stability at more extreme temperatures. At the fourth cycle of reactions, the immobilized laccase exhibited more than 60% relative activity in terms of reusability. Based on the fourier-transform infrared spectroscopy (FTIR) peak at 2921 cm-1, saccharification of paddy straw using immobilized laccase verified lignin degradation. The easy recovery of the immobilized laccase on m-MWCNTs lends credence to its potential use in biomass hydrolysis.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140921166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning: an advancement in biochemical engineering. 机器学习：生物化学工程的进步。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-06-21 DOI: 10.1007/s10529-024-03499-8

Ritika Saha, Ashutosh Chauhan, Smita Rastogi Verma

引用次数: 0

Isolation and characterization of bio-prospecting gut strains Bacillus safensis CGK192 and Bacillus australimaris CGK221 for plastic (HDPE) degradation. 分离和鉴定用于降解塑料（高密度聚乙烯）的生物探究性肠道菌株 Bacillus safensis CGK192 和 Bacillus australimaris CGK221。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-05-06 DOI: 10.1007/s10529-024-03486-z

Kamal Kant Sharma, Himalaya Panwar, Kartikey Kumar Gupta

{"title":"Isolation and characterization of bio-prospecting gut strains Bacillus safensis CGK192 and Bacillus australimaris CGK221 for plastic (HDPE) degradation.","authors":"Kamal Kant Sharma, Himalaya Panwar, Kartikey Kumar Gupta","doi":"10.1007/s10529-024-03486-z","DOIUrl":"10.1007/s10529-024-03486-z","url":null,"abstract":"The present work reports the application of novel gut strains Bacillus safensis CGK192 (Accession No. OM658336) and Bacillus australimaris CGK221 (Accession No. OM658338) in the biological degradation of synthetic polymer i.e., high-density polyethylene (HDPE). The biodegradation assay based on polymer weight loss was conducted under laboratory conditions for a period of 90 days along with regular evaluation of bacterial biomass in terms of total protein content and viable cells (CFU/cm2). Notably, both strains achieved significant weight reduction for HDPE films without any physical or chemical pretreatment in comparison to control. Hydrophobicity and biosurfactant characterization were also done in order to assess strains ability to form bacterial biofilm over the polymer surface. The post-degradation characterization of HDPE was also performed to confirm degradation using analytical techniques such as Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Field emission scanning electronic microscopy (FE-SEM) coupled with energy dispersive X-ray (EDX), and Gas chromatography-mass spectrometry (GC-MS). Interestingly strain CGK221 was found to be more efficient in forming biofilm over polymer surface as indicated by lower half-life (i.e., 0.00032 day-1) and higher carbonyl index in comparison to strain CGK192. The findings reflect the ability of our strains to develop biofilm and introduce an oxygenic functional group into the polymer surface, thereby making it more susceptible to degradation.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140851319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The modification of heme special importer to improve the production of active hemoglobins in Escherichia coli. 改造血红素特殊导入器，提高大肠杆菌活性血红蛋白的产量。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-05-08 DOI: 10.1007/s10529-024-03488-x

Zihan Zhang, Baodong Hu, Tao Zhang, Zhengshan Luo, Jingwen Zhou, Jianghua Li, Jian Chen, Guocheng Du, Xinrui Zhao

{"title":"The modification of heme special importer to improve the production of active hemoglobins in Escherichia coli.","authors":"Zihan Zhang, Baodong Hu, Tao Zhang, Zhengshan Luo, Jingwen Zhou, Jianghua Li, Jian Chen, Guocheng Du, Xinrui Zhao","doi":"10.1007/s10529-024-03488-x","DOIUrl":"10.1007/s10529-024-03488-x","url":null,"abstract":"To enhance the import of heme for the production of active hemoproteins in Escherichia coli C41 (DE3) lacking the special heme import system, heme receptor ChuA from E. coli Nissle 1917 was modified through molecular docking and the other components (ChuTUV) for heme import was overexpressed, while heme import was tested through growth assay and heme sensor HS1 detection. A ChuA mutant G360K was selected, which could import 3.91 nM heme, compared with 2.92 nM of the wild-type ChuA. In addition, it presented that the expression of heme transporters ChuTUV was not necessary for heme import. Based on the modification of ChuA (G360K), the titer of human hemoglobin and the peroxidase activity of leghemoglobin reached 1.19 μg g-1 DCW and 24.16 103 U g-1 DCW, compared with 1.09 μg g-1 DCW and 21.56 103 U g-1 DCW of the wild-type ChuA, respectively. Heme import can be improved through the modification of heme receptor and the engineered strain with improved heme import has a potential to efficiently produce high-active hemoproteins.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140875754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of fadR on the ROS defense mechanism in Shewanalla oneidensis. fadR 对 Shewanalla oneidensis 中 ROS 防御机制的调控。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-05-06 DOI: 10.1007/s10529-024-03487-y

Qiu Meng, Yinming Xu, Liming Dai, Xuzhe Ge, Pei Qiao

引用次数: 0

Preparation of (S)-epichlorohydrin using a novel halohydrin dehalogenase by selective conformation adjustment. 利用新型卤代卤素脱卤酶通过选择性构象调整制备 (S)-环氧氯丙烷。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-05-11 DOI: 10.1007/s10529-024-03479-y

Xiao-Jian Zhang, Meng-Yu Huang, Xin-Xin Peng, Min Cao, Han-Zhong Deng, Yi-Chuan Gong, Xiao-Ling Tang, Zhi-Qiang Liu, Yu-Guo Zheng

{"title":"Preparation of (S)-epichlorohydrin using a novel halohydrin dehalogenase by selective conformation adjustment.","authors":"Xiao-Jian Zhang, Meng-Yu Huang, Xin-Xin Peng, Min Cao, Han-Zhong Deng, Yi-Chuan Gong, Xiao-Ling Tang, Zhi-Qiang Liu, Yu-Guo Zheng","doi":"10.1007/s10529-024-03479-y","DOIUrl":"10.1007/s10529-024-03479-y","url":null,"abstract":"Chiral epichlorohydrin (ECH) is an attractive intermediate for chiral pharmaceuticals and chemicals preparation. The asymmetric synthesis of chiral ECH using 1,3-dicholoro-2-propanol (1,3-DCP) catalyzed by a haloalcohol dehalogenase (HHDH) was considered as a feasible approach. However, the reverse ring opening reaction caused low optical purity of chiral ECH, thus severely restricts the industrial application of HHDHs. In the present study, a novel selective conformation adjustment strategy was developed with an engineered HheCPS to regulate the kinetic parameters of the forward and reverse reactions, based on site saturation mutation and molecular simulation analysis. The HheCPS mutant E85P was constructed with a markable change in the conformation of (S)-ECH in the substrate pocket and a slight impact on the interaction between 1,3-DCP and the enzyme, which resulted in the kinetic deceleration of the reverse reactions. Compared with HheCPS, the catalytic efficiency (kcat(S)-ECH/Km(S)-ECH) of the reversed reaction dropped to 0.23-fold (from 0.13 to 0.03 mM-1 s-1), while the catalytic efficiency (kcat(1,3-DCP)/Km(1,3-DCP)) of the forward reaction only reduced from 0.83 to 0.71 mM-1 s-1. With 40 mM 1,3-DCP as substrate, HheCPS E85P catalyzed the synthesis of (S)-ECH with the yield up to 55.35% and the e.e. increased from 92.54 to >99%. Our work provided an effective approach for understanding the stereoselective catalytic mechanism as well as the green manufacturing of chiral epoxides.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140908122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering of Saccharomyces cerevisiae for high-level production of (+)-ambrein from glucose. 利用代谢工程改造酿酒酵母，从葡萄糖中高水平生产 (+)-ambrein 蛋白。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-06-17 DOI: 10.1007/s10529-024-03502-2

Chumin Lin, Xiaopeng Zhang, Zhongju Ji, Baolian Fan, Yaman Chen, Yuhong Wu, Yuhong Gan, Zhengping Li, Yi Shang, Lixin Duan, Feng Wang

{"title":"Metabolic engineering of Saccharomyces cerevisiae for high-level production of (+)-ambrein from glucose.","authors":"Chumin Lin, Xiaopeng Zhang, Zhongju Ji, Baolian Fan, Yaman Chen, Yuhong Wu, Yuhong Gan, Zhengping Li, Yi Shang, Lixin Duan, Feng Wang","doi":"10.1007/s10529-024-03502-2","DOIUrl":"10.1007/s10529-024-03502-2","url":null,"abstract":"(+)-Ambrein is the primary component of ambergris, a rare product found in sperm whales (Physeter microcephalus). Microbial production using sustainable resources is a promising way to replace animal extraction and chemical synthesis. We constructed an engineered yeast strain to produce (+)-ambrein de novo. Squalene is a substrate for the biosynthesis of (+)-ambrein. Firstly, strain LQ2, with a squalene yield of 384.4 mg/L was obtained by optimizing the mevalonate pathway. Then we engineered a method for the de novo production of (+)-ambrein using glucose as a carbon source by overexpressing codon-optimized tetraprenyl-β-curcumene cyclase (BmeTC) and its double mutant enzyme (BmeTCY167A/D373C), evaluating different promoters, knocking out GAL80, and fusing the protein with BmeTC and squalene synthase (AtSQS2). Nevertheless, the synthesis of (+)-ambrein is still limited, causing low catalytic activity in BmeTC. We carried out a protein surface amino acid modification of BmeTC. The dominant mutant BmeTCK6A/Q9E/N454A for the first step was obtained to improve its catalytic activity. The yield of (+)-ambrein increased from 35.2 to 59.0 mg/L in the shake flask and finally reached 457.4 mg/L in the 2 L fermenter, the highest titer currently available for yeast. Efficiently engineered strains and inexpensive fermentation conditions for the industrial production of (+)-ambrein. The metabolic engineering tools provide directions for optimizing the biosynthesis of other high-value triterpenes.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141330356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxicants improve glycerol production in the fermentation of undetoxified hydrolysate by Candida glycerinogenes. 在甘油酸根念珠菌发酵未解毒水解物的过程中，毒物可提高甘油产量。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-07-31 DOI: 10.1007/s10529-024-03503-1

Xiaohong Zhao, Hong Zong, Xinyao Lu, Bin Zhuge

{"title":"Toxicants improve glycerol production in the fermentation of undetoxified hydrolysate by Candida glycerinogenes.","authors":"Xiaohong Zhao, Hong Zong, Xinyao Lu, Bin Zhuge","doi":"10.1007/s10529-024-03503-1","DOIUrl":"https://doi.org/10.1007/s10529-024-03503-1","url":null,"abstract":"Objectives: Toxicants inhibit microbial fermentation and reduce product titres. This work investigated the glycerol production characteristics of Candida glycerinogenes in highly toxic unwashed undetoxified hydrolysate and provided new ideas for high glycerol production from hydrolysates.Results: The unwashed hydrolysate contains higher concentrations of toxicants, such as furfural, acetic acid, phenols and NaCl than the washed alkali-treated bagasse hydrolysate. C. glycerinogenes fermented unwashed undetoxified hydrolysate yielded 36.1 g/L glycerol, 15.8% higher than the washed hydrolysate, suggesting that the toxicants stimulated glycerol synthesis. qRT-PCR analysis showed that toxicants of unwashed undetoxified hydrolysates greatly up-regulated the transcript levels of the genes GPD1, HXT4 and MSN4 et al. Overexpressing the above genes increased glycerol production by 27.9% to 46.1 g/L. And it was further increased by 8.8% to 50.1 g/L in a 5 L bioreactor.Conclusions: This result proves that toxicants in lignocellulosic hydrolysates can increase the titre of microbial glycerol production.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141858926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A key component Rxt3 in the Rpd3L histone deacetylase complex regulates development, stress tolerance, amylase production and kojic acid synthesis in Aspergillus oryzae. Rpd3L 组蛋白去乙酰化酶复合物中的一个关键成分 Rxt3 调节黑曲霉的发育、抗逆性、淀粉酶的产生和曲酸的合成。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-07-31 DOI: 10.1007/s10529-024-03515-x

Chaofeng Chang, Herui Wang, Yiling Liu, Yiting Xie, Dingxiang Xue, Feng Zhang

引用次数: 0

Efficient genome engineering in Mycolicibacterium neoaurum using Cas9 from Streptococcus thermophilus. 利用嗜热链球菌的 Cas9 在新牛磺酸霉菌中进行高效基因组工程。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-07-31 DOI: 10.1007/s10529-024-03519-7

Gedan Xiang, Tao Liu, Lekai Li, Guihong Lin, Ke Liu, Fengqing Wang

{"title":"Efficient genome engineering in Mycolicibacterium neoaurum using Cas9 from Streptococcus thermophilus.","authors":"Gedan Xiang, Tao Liu, Lekai Li, Guihong Lin, Ke Liu, Fengqing Wang","doi":"10.1007/s10529-024-03519-7","DOIUrl":"https://doi.org/10.1007/s10529-024-03519-7","url":null,"abstract":"Non-pathogenic mycobacteria, including Mycolicibacterium neoaurum, can directly utilize phytosterols for large-scale industrial production of steroid medicine intermediates due to their natural steroid metabolism pathway. The targeted genetic modification of M. neoaurum is conducive to the selection of high-yield engineering bacteria with high-value-added product, such as Pregnadien-20-carboxylic acid (PDC), which is an important precursor for synthesizing some corticosteroids. Based on heterologous type II CRISPR/sth1Cas9 system, a simple strategy was developed to genetic engineer M. neoaurum genome. Here, a customizable plasmid tool pMSC9 was constructed from pMV261 with integration of sth1Cas9 protein and corresponding sgRNA scaffold. Subsequently, the pMSC9 was inserted with spacer sequences corresponding to different targeted genes, generating editing plasmids, and then transformed into M. neoaurum. As a result, the targeted genes were introduced with DNA double stand breaks (DSBs) by CRISPR/sth1Cas9 system and then repaired by innate non-homologous end-joining (NHEJ) mechanism. Finally, editing plasmids were cured from correctly edited M. neoaurum mutants by means of no resistance cultivation, and the resulting mutant deleting the one target gene was used as the host to which another target gene could be deleted via the same process. This study demonstrated that the CRISPR/sth1Cas9 tool allowed M. neoaurum strains to be rapidly edited. And the editing mode of CRISPR/sth1Cas9 system indicated that this tool was an important supplement to the gene editing toolbox of M. neoaurum.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141854653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0