Biotechnology Letters最新文献_第6页

Machine learning: an advancement in biochemical engineering. 机器学习：生物化学工程的进步。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-06-21 DOI: 10.1007/s10529-024-03499-8

Ritika Saha, Ashutosh Chauhan, Smita Rastogi Verma

引用次数: 0

Isolation and characterization of bio-prospecting gut strains Bacillus safensis CGK192 and Bacillus australimaris CGK221 for plastic (HDPE) degradation. 分离和鉴定用于降解塑料（高密度聚乙烯）的生物探究性肠道菌株 Bacillus safensis CGK192 和 Bacillus australimaris CGK221。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-05-06 DOI: 10.1007/s10529-024-03486-z

Kamal Kant Sharma, Himalaya Panwar, Kartikey Kumar Gupta

{"title":"Isolation and characterization of bio-prospecting gut strains Bacillus safensis CGK192 and Bacillus australimaris CGK221 for plastic (HDPE) degradation.","authors":"Kamal Kant Sharma, Himalaya Panwar, Kartikey Kumar Gupta","doi":"10.1007/s10529-024-03486-z","DOIUrl":"10.1007/s10529-024-03486-z","url":null,"abstract":"The present work reports the application of novel gut strains Bacillus safensis CGK192 (Accession No. OM658336) and Bacillus australimaris CGK221 (Accession No. OM658338) in the biological degradation of synthetic polymer i.e., high-density polyethylene (HDPE). The biodegradation assay based on polymer weight loss was conducted under laboratory conditions for a period of 90 days along with regular evaluation of bacterial biomass in terms of total protein content and viable cells (CFU/cm2). Notably, both strains achieved significant weight reduction for HDPE films without any physical or chemical pretreatment in comparison to control. Hydrophobicity and biosurfactant characterization were also done in order to assess strains ability to form bacterial biofilm over the polymer surface. The post-degradation characterization of HDPE was also performed to confirm degradation using analytical techniques such as Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Field emission scanning electronic microscopy (FE-SEM) coupled with energy dispersive X-ray (EDX), and Gas chromatography-mass spectrometry (GC-MS). Interestingly strain CGK221 was found to be more efficient in forming biofilm over polymer surface as indicated by lower half-life (i.e., 0.00032 day-1) and higher carbonyl index in comparison to strain CGK192. The findings reflect the ability of our strains to develop biofilm and introduce an oxygenic functional group into the polymer surface, thereby making it more susceptible to degradation.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"671-689"},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140851319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The modification of heme special importer to improve the production of active hemoglobins in Escherichia coli. 改造血红素特殊导入器，提高大肠杆菌活性血红蛋白的产量。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-05-08 DOI: 10.1007/s10529-024-03488-x

Zihan Zhang, Baodong Hu, Tao Zhang, Zhengshan Luo, Jingwen Zhou, Jianghua Li, Jian Chen, Guocheng Du, Xinrui Zhao

{"title":"The modification of heme special importer to improve the production of active hemoglobins in Escherichia coli.","authors":"Zihan Zhang, Baodong Hu, Tao Zhang, Zhengshan Luo, Jingwen Zhou, Jianghua Li, Jian Chen, Guocheng Du, Xinrui Zhao","doi":"10.1007/s10529-024-03488-x","DOIUrl":"10.1007/s10529-024-03488-x","url":null,"abstract":"To enhance the import of heme for the production of active hemoproteins in Escherichia coli C41 (DE3) lacking the special heme import system, heme receptor ChuA from E. coli Nissle 1917 was modified through molecular docking and the other components (ChuTUV) for heme import was overexpressed, while heme import was tested through growth assay and heme sensor HS1 detection. A ChuA mutant G360K was selected, which could import 3.91 nM heme, compared with 2.92 nM of the wild-type ChuA. In addition, it presented that the expression of heme transporters ChuTUV was not necessary for heme import. Based on the modification of ChuA (G360K), the titer of human hemoglobin and the peroxidase activity of leghemoglobin reached 1.19 μg g-1 DCW and 24.16 103 U g-1 DCW, compared with 1.09 μg g-1 DCW and 21.56 103 U g-1 DCW of the wild-type ChuA, respectively. Heme import can be improved through the modification of heme receptor and the engineered strain with improved heme import has a potential to efficiently produce high-active hemoproteins.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"545-558"},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140875754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of fadR on the ROS defense mechanism in Shewanalla oneidensis. fadR 对 Shewanalla oneidensis 中 ROS 防御机制的调控。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-05-06 DOI: 10.1007/s10529-024-03487-y

Qiu Meng, Yinming Xu, Liming Dai, Xuzhe Ge, Pei Qiao

引用次数: 0

Preparation of (S)-epichlorohydrin using a novel halohydrin dehalogenase by selective conformation adjustment. 利用新型卤代卤素脱卤酶通过选择性构象调整制备 (S)-环氧氯丙烷。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-05-11 DOI: 10.1007/s10529-024-03479-y

Xiao-Jian Zhang, Meng-Yu Huang, Xin-Xin Peng, Min Cao, Han-Zhong Deng, Yi-Chuan Gong, Xiao-Ling Tang, Zhi-Qiang Liu, Yu-Guo Zheng

{"title":"Preparation of (S)-epichlorohydrin using a novel halohydrin dehalogenase by selective conformation adjustment.","authors":"Xiao-Jian Zhang, Meng-Yu Huang, Xin-Xin Peng, Min Cao, Han-Zhong Deng, Yi-Chuan Gong, Xiao-Ling Tang, Zhi-Qiang Liu, Yu-Guo Zheng","doi":"10.1007/s10529-024-03479-y","DOIUrl":"10.1007/s10529-024-03479-y","url":null,"abstract":"Chiral epichlorohydrin (ECH) is an attractive intermediate for chiral pharmaceuticals and chemicals preparation. The asymmetric synthesis of chiral ECH using 1,3-dicholoro-2-propanol (1,3-DCP) catalyzed by a haloalcohol dehalogenase (HHDH) was considered as a feasible approach. However, the reverse ring opening reaction caused low optical purity of chiral ECH, thus severely restricts the industrial application of HHDHs. In the present study, a novel selective conformation adjustment strategy was developed with an engineered HheCPS to regulate the kinetic parameters of the forward and reverse reactions, based on site saturation mutation and molecular simulation analysis. The HheCPS mutant E85P was constructed with a markable change in the conformation of (S)-ECH in the substrate pocket and a slight impact on the interaction between 1,3-DCP and the enzyme, which resulted in the kinetic deceleration of the reverse reactions. Compared with HheCPS, the catalytic efficiency (kcat(S)-ECH/Km(S)-ECH) of the reversed reaction dropped to 0.23-fold (from 0.13 to 0.03 mM-1 s-1), while the catalytic efficiency (kcat(1,3-DCP)/Km(1,3-DCP)) of the forward reaction only reduced from 0.83 to 0.71 mM-1 s-1. With 40 mM 1,3-DCP as substrate, HheCPS E85P catalyzed the synthesis of (S)-ECH with the yield up to 55.35% and the e.e. increased from 92.54 to >99%. Our work provided an effective approach for understanding the stereoselective catalytic mechanism as well as the green manufacturing of chiral epoxides.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"699-711"},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140908122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering of Saccharomyces cerevisiae for high-level production of (+)-ambrein from glucose. 利用代谢工程改造酿酒酵母，从葡萄糖中高水平生产 (+)-ambrein 蛋白。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-06-17 DOI: 10.1007/s10529-024-03502-2

Chumin Lin, Xiaopeng Zhang, Zhongju Ji, Baolian Fan, Yaman Chen, Yuhong Wu, Yuhong Gan, Zhengping Li, Yi Shang, Lixin Duan, Feng Wang

{"title":"Metabolic engineering of Saccharomyces cerevisiae for high-level production of (+)-ambrein from glucose.","authors":"Chumin Lin, Xiaopeng Zhang, Zhongju Ji, Baolian Fan, Yaman Chen, Yuhong Wu, Yuhong Gan, Zhengping Li, Yi Shang, Lixin Duan, Feng Wang","doi":"10.1007/s10529-024-03502-2","DOIUrl":"10.1007/s10529-024-03502-2","url":null,"abstract":"(+)-Ambrein is the primary component of ambergris, a rare product found in sperm whales (Physeter microcephalus). Microbial production using sustainable resources is a promising way to replace animal extraction and chemical synthesis. We constructed an engineered yeast strain to produce (+)-ambrein de novo. Squalene is a substrate for the biosynthesis of (+)-ambrein. Firstly, strain LQ2, with a squalene yield of 384.4 mg/L was obtained by optimizing the mevalonate pathway. Then we engineered a method for the de novo production of (+)-ambrein using glucose as a carbon source by overexpressing codon-optimized tetraprenyl-β-curcumene cyclase (BmeTC) and its double mutant enzyme (BmeTCY167A/D373C), evaluating different promoters, knocking out GAL80, and fusing the protein with BmeTC and squalene synthase (AtSQS2). Nevertheless, the synthesis of (+)-ambrein is still limited, causing low catalytic activity in BmeTC. We carried out a protein surface amino acid modification of BmeTC. The dominant mutant BmeTCK6A/Q9E/N454A for the first step was obtained to improve its catalytic activity. The yield of (+)-ambrein increased from 35.2 to 59.0 mg/L in the shake flask and finally reached 457.4 mg/L in the 2 L fermenter, the highest titer currently available for yeast. Efficiently engineered strains and inexpensive fermentation conditions for the industrial production of (+)-ambrein. The metabolic engineering tools provide directions for optimizing the biosynthesis of other high-value triterpenes.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"615-626"},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141330356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant production of recombinant antigens containing the receptor binding domain (RBD) of two SARS-CoV-2 variants 用植物生产含有两种 SARS-CoV-2 变体受体结合域 (RBD) 的重组抗原

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-07-27 DOI: 10.1007/s10529-024-03517-9

Flavia Fagiani, Rachele Frigerio, Anna Maria Salzano, Andrea Scaloni, Carla Marusic, Marcello Donini

{"title":"Plant production of recombinant antigens containing the receptor binding domain (RBD) of two SARS-CoV-2 variants","authors":"Flavia Fagiani, Rachele Frigerio, Anna Maria Salzano, Andrea Scaloni, Carla Marusic, Marcello Donini","doi":"10.1007/s10529-024-03517-9","DOIUrl":"https://doi.org/10.1007/s10529-024-03517-9","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Objectives</h3>The aim of this work was to rapidly produce in plats two recombinant antigens (RBDw-Fc and RBDo-Fc) containing the receptor binding domain (RBD) of the spike (S) protein from SARS-CoV-2 variants Wuhan and Omicron as fusion proteins to the Fc portion of a murine IgG2a antibody constant region (Fc).<h3 data-test=\"abstract-sub-heading\">Results</h3>The two recombinant antigens were expressed in Nicotiana benthamiana plants, engineered to avoid the addition of N-linked plant-typical sugars, through vacuum agroinfiltration and showed comparable purification yields (about 35 mg/kg leaf fresh weight).<h3 data-test=\"abstract-sub-heading\">Conclusions</h3>Their Western blotting and Coomassie staining evidenced the occurrence of major in planta proteolysis in the region between the RBD and Fc, which was particularly evident in RBDw-Fc, the only antigen bearing the HRV 3C cysteine protease recognition site. The two RBD N-linked glycosylation sites showed very homogeneous profiles free from plant-typical sugars, with the most abundant glycoform represented by the complex sugar GlcNAc4Man3. Both antigens were specifically recognised in Western Blot analysis by the anti-SARS-CoV-2 human neutralizing monoclonal antibody J08-MUT and RBDw-Fc was successfully used in competitive ELISA experiments for binding to the angiotensin-converting enzyme 2 receptor to verify the neutralizing capacity of the serum from vaccinated patients. Both SARS-Cov-2 antigens fused to a murine Fc region were rapidly and functionally produced in plants with potential applications in diagnostics.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"39 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141783584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced hypoxanthine utilization for cAMP salvage synthesis efficiently by Arthrobacter sp. CCTCC 2013431 via xanthine oxidase inhibition 节杆菌 CCTCC 2013431 通过抑制黄嘌呤氧化酶提高利用次黄嘌呤合成 cAMP 的效率

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-07-27 DOI: 10.1007/s10529-024-03513-z

Baofeng Chen, Hai Tan, Chang Li, Linbo Li, Zhonghua Zhang, Zhigang Li

{"title":"Enhanced hypoxanthine utilization for cAMP salvage synthesis efficiently by Arthrobacter sp. CCTCC 2013431 via xanthine oxidase inhibition","authors":"Baofeng Chen, Hai Tan, Chang Li, Linbo Li, Zhonghua Zhang, Zhigang Li","doi":"10.1007/s10529-024-03513-z","DOIUrl":"https://doi.org/10.1007/s10529-024-03513-z","url":null,"abstract":"When hypoxanthine was utilized as the activator for the salvage pathway in cAMP synthesis, xanthine oxidase would generate in quantity leading to low hypoxanthine conversion ratios and cell viability. To enhance cAMP salvage synthesis, fermentations with citrate/luteolin and hypoxanthine coupling added were conducted in a 7 L bioreactor and then multiple physiological indicators of fermentation with luteolin addition were assayed. Due to hypoxanthine feeding, cAMP productivity reached 0.066 g/(L·h) with 43.5% higher than control, however, cAMP synthesis, cell growth and glucose uptake all ceased at 50 h which was shortened by 22 h in comparison to control. The addition of citrate resulted in the cessation of fermentation at 61 h, on the contrary, owing to luteolin addition, cAMP fermentation performance was enhanced significantly during the whole fermentation period (72 h) with higher hypoxanthine conversion ratios and cAMP contents when compared with citrate and only hypoxanthine added batches. Multiple physiological indicators revealed that luteolin inhibited xanthine oxidase activity reducing hypoxanthine decomposition and ROS generation. ATP/AMP, NADH/NAD+ and NADPH/NADP+ were significantly increased especially at the late phase. Moreover, HPRT, PUP expression contents and corresponding gene transcription levels were also elevated. Luteolin could inhibit xanthine oxidase activity and further decrease hypoxanthine decomposition and ROS generation leading to higher hypoxanthine conversion and less cell damage for cAMP salvage synthesis efficiently.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"26 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141783585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a semi-continuous scale-down clone screening model for intensified perfusion culture 为强化灌注培养建立半连续缩减克隆筛选模型

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-07-27 DOI: 10.1007/s10529-024-03512-0

Tao Sun, Yu Zhang, Hengrui Liang, Wenjing Fang, Zichen Qian, Kee Wee Tan, Junjie Li, Xiang Zheng, Mingyue Fang, Hang Zhou, Weichang Zhou, Sam Zhang

{"title":"Establishment of a semi-continuous scale-down clone screening model for intensified perfusion culture","authors":"Tao Sun, Yu Zhang, Hengrui Liang, Wenjing Fang, Zichen Qian, Kee Wee Tan, Junjie Li, Xiang Zheng, Mingyue Fang, Hang Zhou, Weichang Zhou, Sam Zhang","doi":"10.1007/s10529-024-03512-0","DOIUrl":"https://doi.org/10.1007/s10529-024-03512-0","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Purpose</h3>Perfusion cultures have been extensively used in the biotechnology industry to achieve high yields of recombinant products, especially those with stability issue. The WuXiUP™ platform represents a novel intensified perfusion that can achieve ultra‐high productivity. This study describes a representative scale-down 24-deep well plate (24-DWP) cell culture model for intensified perfusion clone screening.<h3 data-test=\"abstract-sub-heading\">Methods</h3>Clonal cell lines were expanded and evaluated in 24-DWP semi-continuous culture. Cell were sampled and counted daily with the aid of an automated liquid handler and high-throughput cell counter. To mimic perfusion culture, 24-DWP plates were spun down and resuspended with fresh medium daily. Top clones were ranked based on growth profiles and productivities. The best performing clones were evaluated on bioreactors.<h3 data-test=\"abstract-sub-heading\">Results</h3>The selected clones achieved volumetric productivity (Pv) up to 5 g/L/day when expressing a monoclonal antibody, with the accumulative harvest Pv exceeding 60 g/L in a 21-day cell culture. Product quality attributes of clones cultured in 24-DWP were comparable with those from bioreactors. A high seeding strategy further shortened the clone screening timeline.<h3 data-test=\"abstract-sub-heading\">Conclusion</h3>In this study, a 24-DWP semi-continuous scale-down model was successfully developed to screen for cell lines suitable for intensified perfusion culture.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"70 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141783586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Native CRISPR-Cas-based programmable multiplex gene repression in Klebsiella variicola 变异克雷伯氏菌中基于 CRISPR-Cas 的原生可编程多重基因抑制

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-07-27 DOI: 10.1007/s10529-024-03516-w

Zhifeng Mo, Siying Lin, Ting Li, Guohui Yu, Yunhao Sun, Jianuan Zhou, Zeling Xu

{"title":"Native CRISPR-Cas-based programmable multiplex gene repression in Klebsiella variicola","authors":"Zhifeng Mo, Siying Lin, Ting Li, Guohui Yu, Yunhao Sun, Jianuan Zhou, Zeling Xu","doi":"10.1007/s10529-024-03516-w","DOIUrl":"https://doi.org/10.1007/s10529-024-03516-w","url":null,"abstract":"Klebsiella variicola is a Gram-negative bacterium that is frequently isolated from a wide variety of natural niches. It is a ubiquitous opportunistic pathogen that can cause diverse infections in plants, animals, and humans. It also has significant biotechnological potential. However, due to the lack of efficient genetic tools, the molecular basis contributing to the pathogenesis and beneficial activities of K. variicola remains poorly understood. In this study, we found and characterized a native type I-E CRISPR-Cas system in a recently isolated K. variicola strain KV-1. The system cannot cleave target DNA sequences due to the inactivation of the Cas3 nuclease by a transposable element but retains the activity of the crRNA-guided Cascade binding to the target DNA sequence. A targeting plasmid carrying a mini-CRISPR to encode a crRNA was designed and introduced into the KV-1 strain, which successfully repurposed the native type I-E CRISPR-Cas system to inhibit the expression of the target gene efficiently and specifically. Moreover, by creating a mini-CRISPR to encode multiple crRNAs, multiplex gene repression was achieved by providing a single targeting plasmid. This work provides the first native CRISPR-Cas-based tool for programmable multiplex gene repression in K. variicola, which will facilitate studying the pathogenic mechanism of K. variicola and enable metabolic engineering to produce valuable bioproducts.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141786056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0