Biotechnology Letters最新文献_第6页

Efficient production of L-asparaginase using recombinant Pichia pastoris and strategy to eliminate glutaminase activity. 利用重组毕赤酵母高效生产l -天冬酰胺酶和消除谷氨酰胺酶活性的策略。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-06-05 DOI: 10.1007/s10529-026-03748-y

Satwika Das, Teena Chandna, Kavya Singh, Maharshi Dixit, Veeranki Venkata Dasu, Surajbhan Sevda, Ashish A Prabhu

{"title":"Efficient production of L-asparaginase using recombinant Pichia pastoris and strategy to eliminate glutaminase activity.","authors":"Satwika Das, Teena Chandna, Kavya Singh, Maharshi Dixit, Veeranki Venkata Dasu, Surajbhan Sevda, Ashish A Prabhu","doi":"10.1007/s10529-026-03748-y","DOIUrl":"10.1007/s10529-026-03748-y","url":null,"abstract":"The mortality rate of Acute Lymphoblastic Leukemia (ALL) has reduced after the incorporation of L-asparaginase (L-ASNase) in therapeutic regimes, which function via selective starvation of the cancer cells, sparing the rest. However, challenges persist due to the immunogenic nature of the enzyme and the co-glutaminase activity. This study investigates the L-ASNase coded for by the ansB gene from Serratia marcescens MTCC 97. An initial study led to a titer of 1.6 U/mL of L-ASNase in a semi synthetic media. However, due to the opportunistic pathogenic nature of the strain and lower yield of the enzyme, a robust eukaryotic host, and the Pichia pastoris GlycoSwitch® SuperMan5 (pep4-prb1-) strain was selected for the expression. The gene was codon optimized to further enhance the yield. The recombinant strain produced 16.6 U/mL of L-ASNase in a complex BMMY medium. Process and parameter optimization were done via statistical design using the Taguchi orthogonal array, leading to a final yield of 22.3 U/mL of L-ASNase, obtained at 37 °C, at a strictly neutral pH, when 1% inoculum was used to initiate the fermentation, with 0.5% v/v methanol induction. Batch bioreactor cultivation further enhanced the L-ASNase titer to 31.75 U/mL. However, the L-ASNase was also found to have a glutaminase activity, for which in-silico characterization of the enzyme was done for an elevated understanding of the intricate structure and active sites. Through modelling and simulation studies, possible mutations were explored with an aim to reduce the glutaminase activity, and thereby reduce the secondary function of the L-ASNase.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148161268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved thermostability of Thermobifida fusca mannanase obtained by error-prone PCR mutagenesis. 通过容易出错的PCR诱变获得的改良的fusca甘露聚糖酶的热稳定性。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-06-03 DOI: 10.1007/s10529-026-03749-x

Yawei Wang, Qiang Xue, Guan Wang, Zhenggang Han, Jiangke Yang, Hairong Xiong

{"title":"Improved thermostability of Thermobifida fusca mannanase obtained by error-prone PCR mutagenesis.","authors":"Yawei Wang, Qiang Xue, Guan Wang, Zhenggang Han, Jiangke Yang, Hairong Xiong","doi":"10.1007/s10529-026-03749-x","DOIUrl":"10.1007/s10529-026-03749-x","url":null,"abstract":"A thermostable β-Mannanase (ManB, PDB code 1BQC, gene code KJ806638) from Thermobifida fusca was expressed in Escherichia coli BL21. The error-prone PCR of ManB gene were adopted to obtain three special mutants with the sites of N15I (M1), D40G/T159I (M2), and H28Q/V57D (M3). It was found that the optimum temperature of wild type ManB was 72.5 °C, whereas of two mutants M1 and M2 were 77.5 °C and mutant M3 was 70 °C. The apparent melting temperatures (Tm) measured by differential scanning fluorimetry (DSF). The Tm values of ManB was 61.9 °C, as well the mutants M1, M2 and M3 were 63.2 °C, 63.4 °C and 48.7 °C, respectively. With guar gum as the substrate, the Km of the original ManB and the mutants M1, M2 and M3 were 0.74, 0.79, 1.28, 0.70 mg/ml, as well the specific activity of them were 1293, 1344, 1728, 446 IU/mg, respectively. Stick models and molecular dynamics analysis show that structural stabilization, particularly in the active cleft region, improves the thermostability of the enzyme. Those specific mutated sites of N15I (M1), D40G/T159I (M2), and H28Q/V57D (M3) are novel and provide the additional structure understanding of mannanase 1BQC, as well the GROMACS module demonstrated the molecular structure modification of mannanase in the active cleft region effect the stability significantly.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148148193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immobilization of Endonuclease A from Serratia marcescens on cellulose membranes. 粘质沙雷氏菌内切酶A在纤维素膜上的固定化。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-06-03 DOI: 10.1007/s10529-026-03728-2

Tobias Schneider, Michael Wolff

{"title":"Immobilization of Endonuclease A from Serratia marcescens on cellulose membranes.","authors":"Tobias Schneider, Michael Wolff","doi":"10.1007/s10529-026-03728-2","DOIUrl":"10.1007/s10529-026-03728-2","url":null,"abstract":"The removal of ds-DNA constitutes an indispensable step in numerous biotechnological production processes. In many cases, endonucleases are employed for this purpose. The objective of this study was to investigate the immobilization of Endonuclease A from Serratia marcescens onto cellulose filters, which could later be used in biotechnological production processes. The filters were treated with p-toluenesulfonyl chloride prior to enzyme functionalization, and tosyl loading was confirmed by HPLC. Enzyme activity after immobilization was investigated using a colorimetric assay. The functionalization of the filters was most effective when they were treated with 0.5 M NaOH prior to reaction with p-toluenesulfonyl chloride. The subsequent endonuclease immobilization process was screened under various pH conditions and was most successful when conducted at a slightly acidic pH. However, it was also observed that functionalization with p-toluenesulfonyl chloride was not necessary to immobilize the endonuclease onto the cellulose membranes, as initially assumed. This indicates that immobilization can proceed through a pathway independent of the presence of tosylate groups on the surface of the cellulose filters.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13233652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148148212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-level secretory production of a highly hydrophilic human-like type III collagen in Komagataella phaffii by a multicopy genomic integration strategy. 通过多拷贝基因组整合策略，高水平分泌法菲Komagataella III型胶原蛋白的高度亲水性。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-05-30 DOI: 10.1007/s10529-026-03746-0

Wanting Wu, Junhao Yue, Yixuan Jia, Chuang Xing, Yu Deng, Changjiang Lyu

{"title":"High-level secretory production of a highly hydrophilic human-like type III collagen in Komagataella phaffii by a multicopy genomic integration strategy.","authors":"Wanting Wu, Junhao Yue, Yixuan Jia, Chuang Xing, Yu Deng, Changjiang Lyu","doi":"10.1007/s10529-026-03746-0","DOIUrl":"10.1007/s10529-026-03746-0","url":null,"abstract":"Collagen, a key extracellular matrix protein, regulates cell adhesion, proliferation, and tissue integrity for skin health. In this study, a novel human-like type III collagen was engineered by substituting hydrophobic GXY triplets in the core fragment (Gly201-Asn498) of the human α1(III)type chain with hydrophilic motifs (GSP, GQP, GEP, and GSQ) and incorporating integrin-binding sequences (GER and RGD) at the C-terminus of the two consecutive sequence units. The codon-optimized gene was cloned into the pPIC9K vector, and transformed into Komagataella phaffii (Pichia pastoris) GS115. Multicopy transformants were selected by various G418 concentrations. Furthermore, a high yield of recombinant collagen at 19.4 g/L was achieved by fed-batch fermentation in a 5-L bioreactor. The purified protein exhibited excellent thermostability, remaining fully soluble after heating at 121 °C for 30 min. The collagen migrated on 10% SDS-PAGE with an apparent molecular weight approximately 2.18-fold greater than its theoretical mass, suggesting extensive hydration. Functionally, it significantly promoted the proliferation of human skin fibroblasts (HSF) and immortalized keratinocytes (HaCaT). In HSF cells, it upregulated the expression of COL1A1, COL3A1, and TIMP1, while in HaCaT cells, it enhanced transcription of skin barrier-related genes, including KRT1, KRT5, KRT10, KRT14, IVL, LOR, and FLG. This designed collagen integrates high-yield production, thermostability, and dual bioactivity for dermal regeneration and epidermal barrier reinforcement, showing a promising application for skincare and tissue engineering.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148101044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-fits-all fluorescence in situ hybridisation (FISH) with single and high-copy DNA probes for Arecoideae species. 一种适合所有的荧光原位杂交（FISH）与单一和高拷贝DNA探针Arecoideae物种。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-05-28 DOI: 10.1007/s10529-026-03745-1

Noorhariza Mohd Zaki, Trude Schwarzacher, Pat Heslop-Harrison, Hanim Mohd Nor Nordiana, Rajinder Singh

{"title":"One-fits-all fluorescence in situ hybridisation (FISH) with single and high-copy DNA probes for Arecoideae species.","authors":"Noorhariza Mohd Zaki, Trude Schwarzacher, Pat Heslop-Harrison, Hanim Mohd Nor Nordiana, Rajinder Singh","doi":"10.1007/s10529-026-03745-1","DOIUrl":"10.1007/s10529-026-03745-1","url":null,"abstract":"Fluorescent in situ hybridisation (FISH) has been the most powerful and versatile technique in cytogenetics research to answer and validate biological questions associated with the structure, organisation, variation, and evolution of individual chromosomes and entire genomes. A standard reference FISH approach that requires little or no modifications is desired to minimise the time and costs in fine-tuning the cumbersome FISH approach. However, the available published FISH methods do not provide a comprehensive step-by-step technique that works across Arecoideae taxa. Here, using single- and high-copy DNA probes, we report a FISH approach developed for Elaeis guineensis that gave reproducible results in three commercially important Arecoidae species: Elaeis oleifera, Cocos nucifera and Phoenix dactylifera. Our protocol covers detailed experimentation steps from preparing high-quality chromosome spreads to localising the different DNA probes on chromosomes of the selected species. We propose the established method here as a reference platform for research communities to embark on the molecular cytogenetics-related study in oil palm and other distantly related species within over 100 genera of the Arecoideae, the largest subfamily in Arecaceae.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148040657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding the cofactor range of aldehyde reductase YqhD by mutating key amino acids. 通过突变关键氨基酸扩大醛还原酶YqhD的辅因子范围。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-05-25 DOI: 10.1007/s10529-026-03747-z

Xiaohui Wu, Yinghan Hu, Yanzhe Huang, Lingyun Zhang, Yan Xue, Hailong Cao, Xueying Wang, Yongjin J Zhou

{"title":"Expanding the cofactor range of aldehyde reductase YqhD by mutating key amino acids.","authors":"Xiaohui Wu, Yinghan Hu, Yanzhe Huang, Lingyun Zhang, Yan Xue, Hailong Cao, Xueying Wang, Yongjin J Zhou","doi":"10.1007/s10529-026-03747-z","DOIUrl":"10.1007/s10529-026-03747-z","url":null,"abstract":"YqhD is a broad-substrate aldehyde reductase that natively prefers NADPH and is widely used in alcohol biosynthesis. However, insufficient NADPH supply and low cofactor regeneration efficiency limit its catalytic performance. To address this limitation, we engineered YqhD through structure-guided semi-rational design and obtained a mutant G37D with markedly improved dual-cofactor compatibility with NADPH and NADH. With butanal and 3-hydroxypropionaldehyde as substrates, G37D exhibited 2.6- and 4.8-fold increases in catalytic efficiency (kcat/Km) with NADH as the cofactor, while retaining > 70% of the wild-type efficiency with NADPH, resulting in comparable efficiencies with both cofactors. Further structural analysis and molecular dynamics simulations revealed that the G37D mutation enhances dual-cofactor utilization by reprogramming the hydrogen-bond network and stabilizing the catalytically favorable closed conformation. This study provides an improved enzyme module for the biomanufacturing of 1,3-propanediol and related alcohols, and offers new insights into the design of versatile catalysts for diverse biocatalytic scenarios.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148013337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and phenotypic characterization of seedling drought tolerance in wheat with integrated analysis of trait variation and QTL mapping. 小麦幼苗抗旱性的遗传表型与性状变异分析及QTL定位。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-05-23 DOI: 10.1007/s10529-026-03742-4

Aylin Zebarjad, Hossein Sabouri, Zahra Pezeshkian, Fakhtak Taliei, Sayed Javad Sajadi, Borzo Kazerani

{"title":"Genetic and phenotypic characterization of seedling drought tolerance in wheat with integrated analysis of trait variation and QTL mapping.","authors":"Aylin Zebarjad, Hossein Sabouri, Zahra Pezeshkian, Fakhtak Taliei, Sayed Javad Sajadi, Borzo Kazerani","doi":"10.1007/s10529-026-03742-4","DOIUrl":"10.1007/s10529-026-03742-4","url":null,"abstract":"Wheat (Triticum aestivum L.) is a staple cereal crop highly vulnerable to drought stress, particularly during early seedling establishment, which limits productivity and yield stability. This study evaluated a recombinant inbred line population under well-watered and drought environments to dissect the morphological, physiological, and genetic bases of seedling drought tolerance. Drought stress severely reduced shoot and leaf biomass, while paradoxically enhancing root growth, suggesting adaptive resource reallocation to improve water foraging. Genetic variance analyses uncovered increased heritable variation for root traits under stress, alongside significant genotype-by-environment interactions, indicative of distinct adaptive responses. Multivariate analyses revealed an integrated stress-response network linking stay-gFreen phenotypes and biomass maintenance. Importantly, QTL mapping identified twelve drought-specific loci most notably on chromosomes 2A, 3B, 5A, and 6A as well as eight loci unique to non-stress conditions, underscoring environment-dependent genetic architectures. Major QTLs affecting seedling length, root length, and leaf senescence offer promising targets for marker-assisted breeding. Collectively, these results illuminate the complex, multigenic control of early-stage drought tolerance in wheat, providing a robust foundation for developing cultivars with improved resilience in water-limited environments and strengthening climate-smart breeding pipelines.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148004491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of continuous light and skeleton photoperiods on photosynthesis and circadian rhythm in celery. 连续光照和骨架光周期对芹菜光合作用和昼夜节律的影响。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-05-18 DOI: 10.1007/s10529-026-03740-6

Chen Chen, Ting Huang, Li-Xiang Wang, Meng-Yao Li, Guo-Fei Tan, Jing Zhuang, Jin-Song Xiong, Jun Yan, Ai-Sheng Xiong

{"title":"Effects of continuous light and skeleton photoperiods on photosynthesis and circadian rhythm in celery.","authors":"Chen Chen, Ting Huang, Li-Xiang Wang, Meng-Yao Li, Guo-Fei Tan, Jing Zhuang, Jin-Song Xiong, Jun Yan, Ai-Sheng Xiong","doi":"10.1007/s10529-026-03740-6","DOIUrl":"10.1007/s10529-026-03740-6","url":null,"abstract":"Photoperiod plays a pivotal role in regulating plant physiological cycles and photosynthetic processes via the circadian clock. Celery is an important leafy vegetable with high sensitivity to photoperiod. However, how photosynthesis and circadian rhythms respond to different photoperiod patterns remains to be fully explored in this species. To investigate the effects of different photoperiods on photosynthesis and circadian rhythm in celery, we applied equinoctial photoperiod (12 h of light/12 h of dark, 12L12D), continuous light (24 h of light, 24L) and two skeleton photoperiods (6 h of light/6 h of dark, 6L6D and 3 h of light/3 h of dark, 3L3D). Under the 12L12D, stomatal aperture, chlorophyll content, photosynthetic parameters, and the expression pattens of circadian rhythm-related genes all exhibited robust 24-h rhythmic patterns. Continuous light (24L) increased the daily mean chlorophyll and nitrogen contents to 1.1-fold than those observed under 12L12D, while stabilizing photosynthetic parameters and stomatal apertures with minimal daily variation. Conversely, skeletal photoperiods (6L6D and 3L3D) rapidly disrupted these established patterns, the expression of circadian rhythm-related genes and photosynthetic parameters exhibited arrhythmic fluctuations. Our findings demonstrated that extended light exposure within a 24-h framework optimized photosynthetic performance in celery, whereas skeleton photoperiods impaired circadian rhythm and photosynthesis-related output.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147970112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of harvesting method on biomass yield and antimicrobial activity of Isochrysis galbana and Phaeodactylum tricornutum extracts. 采收方式对甘露等chrysis galbana和三角褐指草提取物生物量及抗菌活性的影响。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-05-16 DOI: 10.1007/s10529-026-03741-5

Edgard Freitas de Siqueira Junior, Luzimar Gonzaga Fernandez, Edson Delgado Rodrigues, Suzana Telles da Cunha Lima

{"title":"Influence of harvesting method on biomass yield and antimicrobial activity of Isochrysis galbana and Phaeodactylum tricornutum extracts.","authors":"Edgard Freitas de Siqueira Junior, Luzimar Gonzaga Fernandez, Edson Delgado Rodrigues, Suzana Telles da Cunha Lima","doi":"10.1007/s10529-026-03741-5","DOIUrl":"10.1007/s10529-026-03741-5","url":null,"abstract":"Microalgae are recognized as promising sources of high-value bioactive compounds with applications in agricultural and pharmacological industries; however, conventional biomass harvesting methods may reduce yield, compromise bioactivity, and increase processing costs. This study aimed to evaluate the effect of electroflocculation on biomass recovery and the preservation of antimicrobial activity of microalgal extracts, in comparison with centrifugation. Cultures of both species were harvested using electroflocculation and centrifugation, followed by biomass quantification and extraction of bioactive compounds. The antibacterial activity of the extracts was assessed against bacterial strains of clinical and industrial relevance, allowing a comparative evaluation of harvesting efficiency and bioactivity preservation. Electroflocculation resulted in higher biomass recovery compared to centrifugation for both microalgal species. In addition, extracts obtained from electroflocculated biomass exhibited higher antibacterial activity under the tested conditions, indicating improved preservation of bioactive compounds during the harvesting process. These findings demonstrate that electroflocculation is a clean, efficient, and sustainable method for microalgal biomass harvesting, suggesting advantages over conventional centrifugation in terms of yield and maintenance of antimicrobial activity, and supporting its application in biotechnological processes aimed at producing bioactive compounds from microalgae.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13179900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147959700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing camptothecin biosynthesis in Camptotheca acuminata through CrLAMT transfer from Catharanthus roseus. 通过从玫瑰花中转移CrLAMT促进喜树碱的生物合成。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-05-15 DOI: 10.1007/s10529-026-03737-1

Ru Huang, Wen-Hui Ma, Bing-Run Yang, Fang Yu, Ping Kou

{"title":"Enhancing camptothecin biosynthesis in Camptotheca acuminata through CrLAMT transfer from Catharanthus roseus.","authors":"Ru Huang, Wen-Hui Ma, Bing-Run Yang, Fang Yu, Ping Kou","doi":"10.1007/s10529-026-03737-1","DOIUrl":"10.1007/s10529-026-03737-1","url":null,"abstract":"Camptothecin (CPT), a monoterpene indole alkaloid with significant anticancer properties, is primarily sourced from Camptotheca acuminata. However, its complex chemical structure and low natural accumulation hinder large-scale clinical applications. To enhance CPT production, this study explored the heterologous expression of CrLAMT, a O-methyltransferase from Catharanthus roseus in C. acuminata. The introduced CrLAMT successfully activated an alternative methylated intermediate pathway in C. acuminata, enabling the conversion of loganic acid to loganin. This dual-pathway approach modification increased CPT content in both leaves and hairy roots, with a 2.23-fold and 1.78-fold increase observed in transgenic materials compared to controls. In addition, CaSTR2, a key downstream enzyme in the CPT biosynthesis pathway, was significantly up-regulated. The study demonstrates that the overexpression of CrLAMT not only enhances CPT production but also optimizes the utilization of intermediate metabolites. This metabolic engineering strategy provides a novel approach to improving the yield of natural products like CPT, and also provides valuable insights for promoting the metabolism of other high value natural products.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147947948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0