Biotechnology Letters最新文献_第7页

Expression of nisin in Scenedesmus acutus and evaluation of its antimicrobial activity. nisin在针叶草中的表达及抑菌活性评价。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-06-12 DOI: 10.1007/s10529-025-03603-6

Julieta Ponce-Alonso, Luzmila Martínez-González, Ángel Gabriel Alpuche-Solís, Fidel Martinez-Gutierrez, Ana Cecilia Lorenzo-Leal, Horacio Bach, Ruth Elena Soria-Guerra

{"title":"Expression of nisin in Scenedesmus acutus and evaluation of its antimicrobial activity.","authors":"Julieta Ponce-Alonso, Luzmila Martínez-González, Ángel Gabriel Alpuche-Solís, Fidel Martinez-Gutierrez, Ana Cecilia Lorenzo-Leal, Horacio Bach, Ruth Elena Soria-Guerra","doi":"10.1007/s10529-025-03603-6","DOIUrl":"10.1007/s10529-025-03603-6","url":null,"abstract":"Objectives: The study aimed to express a synthetic nisin gene in the nucleus of the green microalga Scenedesmus acutus UTEX 72 and evaluate its antimicrobial activity in vitro against Staphylococcus aureus (ATCC 29213), Enterococcus faecalis (ATCC 29212), and Listeria monocytogenes (ATCC 19118).Results: Nuclear transformation of S. acutus was achieved through co-culture with Agrobacterium tumefaciens. PCR analysis confirmed the presence of the Nis gene in the transformant lines, while ELISA quantified the total protein content. Results showed that nisin was predominantly expressed in the total soluble protein fraction, with the highest yield reaching 2.24 µg/µl. Antimicrobial assays demonstrated growth inhibition in all three tested Gram-positive bacteria. S. aureus and L. monocytogenes were inhibited after 60 min of exposure to 14.3 µg/ml of transgenic nisin, whereas E. faecalis needed 55 µg/ml and 180 min for inhibition. No antimicrobial activity was observed with wild-type S. acutus total soluble protein.Conclusions: Nisin was successfully expressed in the nucleus of S. acutus, exhibiting antimicrobial activity against S. aureus, L. monocytogenes, and E. faecalis. These findings highlight S. acutus as a promising platform for the production of transgenic antimicrobial peptides, offering a potential alternative for combating pathogenic bacteria.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 3","pages":"60"},"PeriodicalIF":2.0,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RETRACTED ARTICLE: High-level expression of recombinant human vascular endothelial growth factor (rhVEGF165) in silkworm larvae (Bombyx mori). 撤回：重组人血管内皮生长因子（rhVEGF165）在家蚕幼虫中的高水平表达。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-06-01 Epub Date: 2007-07-13 DOI: 10.1007/s10529-007-9464-9

Tingmei Ye, Peng Cao, Jiang Zhang, Zhihua Lin

引用次数: 0

Carrier immobilization and auto-exposition favoring reuse of silyletherase SilE-R from Brassica sp. with high activity and enantiospecificity. 具有高活性和对映体特异性的芸苔属硅醚化酶SilE-R的载体固定化和自体暴露。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-05-29 DOI: 10.1007/s10529-025-03600-9

Lisa Pick, Anna L Schumacher, Elif Öztel, Thorsten Mascher, Marion B Ansorge-Schumacher

{"title":"Carrier immobilization and auto-exposition favoring reuse of silyletherase SilE-R from Brassica sp. with high activity and enantiospecificity.","authors":"Lisa Pick, Anna L Schumacher, Elif Öztel, Thorsten Mascher, Marion B Ansorge-Schumacher","doi":"10.1007/s10529-025-03600-9","DOIUrl":"10.1007/s10529-025-03600-9","url":null,"abstract":"Objectives: Investigation of immobilization methods promoting the use of silyletherases from Brassica sp. for the efficient and enantiospecific hydrolysis of silyl-protected hydroxyl functions.Results: Different supports for adsorptive and covalent binding of the silyletherase SilE-R as well as exposure of the enzyme on the surface of Bacillus subtilis endospores, so-called SporoBeads, were evaluated. While the highest protein loading of 26 mg enzyme per gram was obtained by adsorptive binding, the best combination of specific activity and enantiospecificity was obtained when SilE-R was exposed on SporoBeads. Protein loading was estimated at 2.6 mg per gram of spore, which was in the same range as after covalent binding to a carrier. In six repeated reaction cycles, SporoBeads exposing SilE-R lost less than 10% of their catalytic activity. The enantiomeric excess could not be increased even with short reaction times, but remained constant over all repeated cycles.Conclusion: The exposure of silyletherases on SporoBeads has been identified as a promising approach for the synthetic application of this novel type of enzyme, although some properties relevant for catalytic applications need to be further improved.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 3","pages":"59"},"PeriodicalIF":2.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterologous expression of flavohemoglobin in Rhodotorula toruloides led to improved lipid production. 异种表达黄血红蛋白的红tortorula toruloides导致提高脂质生产。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-05-26 DOI: 10.1007/s10529-025-03597-1

Shouping Lai, Liting Lyu, Aabid Manzoor Shah, Qiongqiong Chen, Shuang Wang, Hongying Wang, Zongbao Kent Zhao

{"title":"Heterologous expression of flavohemoglobin in Rhodotorula toruloides led to improved lipid production.","authors":"Shouping Lai, Liting Lyu, Aabid Manzoor Shah, Qiongqiong Chen, Shuang Wang, Hongying Wang, Zongbao Kent Zhao","doi":"10.1007/s10529-025-03597-1","DOIUrl":"10.1007/s10529-025-03597-1","url":null,"abstract":"The red yeast Rhodotorula toruloides emerged as a prominent host for microbial lipid production. It has been known that oxygen supply is a limiting factor for de novo lipid biosynthesis especially under high cell-density culture conditions. Bacterial hemoglobin is an oxygen-binding protein that can actively transport oxygen from the environment to metabolic processes. The aim of the present study was to address the problem of oxygen limitation during R. toruloides culture thereby enhancing cell growth and lipid synthesis. Thus, genes encoding flavohemoglobin (SHb) from Sinorhizobium meliloti and hemoglobin (VHb) from Vitreoscilla sp. were separately integrated into the genome of R. toruloides CGMCC 2.1389 by Agrobacterium-mediated transformation method. It was found that the engineered strain 4S-8 with SHB gene integration produced 49% more lipids under shake-flask culture condition than the parent strain. Under the two-stage culture process, the engineered strain 4S-8 produced 23% and 10% more cell mass than the parent strain and the strain 4V-10 with VHB gene integration, respectively, while the lipid titer of strain 4S-8 was 42% higher than that of the parent strain. These results suggested that heterologous expression of SHb in oleaginous hosts may be applicable for improved production of lipids and cell mass, which should facilitate more efficient conversion of sugars into lipids and fatty-acid derived biofuels.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 3","pages":"58"},"PeriodicalIF":2.0,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing terpenoid production in Saccharomyces cerevisiae via cell morphology engineering. 利用细胞形态学工程提高酿酒酵母萜类化合物的产量。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-05-17 DOI: 10.1007/s10529-025-03584-6

Ke Bi, Siqi Zuo, Jiazhang Lian, Zhinan Xu

引用次数: 0

Improvement of transglutaminase production by controlling the morphology of Streptomyces mobaraensis HVCP-Sm1 with microparticle-enhanced cultivation. 微颗粒强化培养控制莫巴拉链霉菌HVCP-Sm1形态提高转谷氨酰胺酶产量

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-05-17 DOI: 10.1007/s10529-025-03598-0

Ting-Ting Chang, Lu-Yao Bian, Chong Zhang

{"title":"Improvement of transglutaminase production by controlling the morphology of Streptomyces mobaraensis HVCP-Sm1 with microparticle-enhanced cultivation.","authors":"Ting-Ting Chang, Lu-Yao Bian, Chong Zhang","doi":"10.1007/s10529-025-03598-0","DOIUrl":"10.1007/s10529-025-03598-0","url":null,"abstract":"Objective: Transglutaminase (TGase) is widely utilized in food, textile, and medical applications due to its superior capacity to facilitate protein cross-linking. The mechanisms driving high TGase production in Streptomyces mobaraensis require further exploration. \"Microparticle-Enhanced Cultivation\" (MPEC), has been shown to significantly influence the morphology of filamentous microorganisms, thereby impacting secondary metabolism.Result: In the study, CaCO3 and talc were found to increase the TGase activity of HVCP-Sm1 from 15 U/mL to 29.05 U/mL and 31 U/mL respectively. Moreover, CaCO3 and talc were observed to reduce the diameters of hyphal particles and elongated hyphae while promoting pro-TGase activation. This research expands the understanding of the role of MPEC in the morphology of filamentous microorganisms and its effects on secondary metabolism.Conclusion: The synergistic effects of group sensing, energy metabolism, and the optimization of mycelial morphology enhanced the secondary metabolism of HVCP-Sm1 and increased TGase enzymatic activity.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 3","pages":"57"},"PeriodicalIF":2.0,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant molecular farming: a promising frontier for orphan drug production. 植物分子农业：孤儿药生产的一个有前途的前沿。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-05-17 DOI: 10.1007/s10529-025-03596-2

Balamurugan Shanmugaraj, Kavin Ravi, Kishore Baladevan

{"title":"Plant molecular farming: a promising frontier for orphan drug production.","authors":"Balamurugan Shanmugaraj, Kavin Ravi, Kishore Baladevan","doi":"10.1007/s10529-025-03596-2","DOIUrl":"10.1007/s10529-025-03596-2","url":null,"abstract":"Orphan diseases comprise a range of disorders that individually affect a small percentage of people, but collectively impact millions of people worldwide. Patients with this disorder often face significant challenges in diagnosis, treatment, and access to care due to their rare nature and limited understanding and treatment options. In recent years, significant advancements have been made in the global healthcare in addressing the accessibility of essential treatments and medicines, but still challenges persist particularly related to orphan drugs (to treat rare diseases) in the developing world. The accessibility of orphan drugs remains a major challenge, where patients face barriers such as high costs, limited availability, and inadequate healthcare infrastructure. The high cost associated with orphan drugs presents a barrier to affordability for both patients and healthcare systems, causing disparities in access to life-saving treatments. The molecular farming approach utilizing plant-based production systems for recombinant protein production offers a hope for overcoming barriers to orphan drug access in resource-constrained settings. Molecular farming has the potential to produce a wide range of therapeutic proteins and biologics for the treatment of various rare diseases. The FDA approval of plant-derived proteins for the treatment of Gaucher disease (Elelyso) and Fabry disease (Elfabrio) highlights the potential of plant-based expression systems for the development of suitable drugs targeting niche and orphan diseases. This review examines the potential of the plant system in producing orphan drugs and also highlights the opportunities and challenges related to orphan drug manufacturing.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 3","pages":"56"},"PeriodicalIF":2.0,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a high-throughput scale-down clone screening platform for intensified fed-batch culture of CHO cells. CHO细胞强化补料批培养的高通量缩小克隆筛选平台的建立。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-05-08 DOI: 10.1007/s10529-025-03573-9

Haiyan Luo, Shuai Wang, Collin Chong, Lile Wang, Xiaojun Sun, Qian Guo, Sam Zhang, Xiaoyue Chen, Hang Zhou, Weichang Zhou

{"title":"Establishment of a high-throughput scale-down clone screening platform for intensified fed-batch culture of CHO cells.","authors":"Haiyan Luo, Shuai Wang, Collin Chong, Lile Wang, Xiaojun Sun, Qian Guo, Sam Zhang, Xiaoyue Chen, Hang Zhou, Weichang Zhou","doi":"10.1007/s10529-025-03573-9","DOIUrl":"10.1007/s10529-025-03573-9","url":null,"abstract":"Purpose: To develop a scale-down clone screening platform for the intensified fed-batch (IFB) process to allow efficient identification of high expressing clones fitting the IFB culture strategy in bioreactor.Results: Three monoclonal antibodies (mAbs) were used in the development and validation of the IFB specific clone screening platform for CHO cells. The IFB platform significantly improved titer levels, achieving an average titer of 8 g/L and the highest titer of 9.6 g/L. With similar cell viability, lactate profile and titer levels, both the spin tube model and the AMBR250@ bioreactor system were effective in screening clones suitable for IFB process. The addition of aurintricarboxylic acid (ATA) and uridine in the process optimization led to a further increase in expression levels in both systems, achieving the highest titer of 12.2 g/L.Conclusion: This IFB-process specific clone screening serves as an alternative platform for industry application that can increase the effectiveness and efficiency of screening high-expressing CHO cell lines for IFB production.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 3","pages":"54"},"PeriodicalIF":2.0,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing Yarrowia lipolytica sub-organelle engineering with endogenous mitochondrial targeting sequence. 利用内源性线粒体靶向序列推进多脂耶氏菌亚细胞器工程。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-05-08 DOI: 10.1007/s10529-025-03590-8

Krutika Bhoir, K J Hemavathi, Gunjan Prakash

{"title":"Advancing Yarrowia lipolytica sub-organelle engineering with endogenous mitochondrial targeting sequence.","authors":"Krutika Bhoir, K J Hemavathi, Gunjan Prakash","doi":"10.1007/s10529-025-03590-8","DOIUrl":"10.1007/s10529-025-03590-8","url":null,"abstract":"Objective: The aim of the study was identification and validation of an endogenous mitochondrial targeting signal (MTS) sequence of Yarrowia lipolytica, for efficient compartmentalization of a target protein to mitochondria.Results: MTS from citrate synthase of Y. lipolytica (YlCISY-MTS) was identified, isolated and fused with green fluorescent protein (GFP) to direct it to the mitochondrial matrix. The efficiency of localization of GFP to mitochondrial matrix with YlCISY-MTS was compared with currently used MTS from Saccharomyces cerevisiae's cytochrome oxidase subunit IV. Confocal microscopy confirmed the targeted and greater GFP localization, underlining the potential of endogenous YlCISY-MTS for mitochondrial engineering in Y. lipolytica. The availability of endogenous MTS will evade the need of codon optimization of S. cerevisiae MTS for mitochondrial engineering in Y. lipolytica. This is the first report of an endogenous MTS of Y. lipolytica.Conclusion: An endogenous MTS of Y. lipolytica has been identified to facilitate the targeted delivery of a protein in the mitochondria enabling future advancements through leveraging the unique subcellular environment for metabolic engineering applications.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 3","pages":"53"},"PeriodicalIF":2.0,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new strategy for the fermentation of Massa Medicata Fermentata by combining multiple strains of fermentation and their fermentation mechanisms. 多菌种组合发酵马尾草的新策略及其发酵机制。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2025-05-07 DOI: 10.1007/s10529-025-03595-3

Wanqiu Liu, Ying Liu, Jie Zhang, Huangyao Zhu, Siqi Fan, Jingwen Ha, Yuxuan Lu, Yizhu Wang, Wenyuan Liu, Ma Mi, Feng Feng, Jian Xu

{"title":"A new strategy for the fermentation of Massa Medicata Fermentata by combining multiple strains of fermentation and their fermentation mechanisms.","authors":"Wanqiu Liu, Ying Liu, Jie Zhang, Huangyao Zhu, Siqi Fan, Jingwen Ha, Yuxuan Lu, Yizhu Wang, Wenyuan Liu, Ma Mi, Feng Feng, Jian Xu","doi":"10.1007/s10529-025-03595-3","DOIUrl":"10.1007/s10529-025-03595-3","url":null,"abstract":"To address the quality instability of the traditional fermentation process of Massa Medicata Fermentata (LSQ), we designed an innovation strategy for dual-strain co-fermentation LSQ. Rhizopus arrhizus, Bacillus velezensis, Bacillus subtills, and Bacillus cereus were selected as the fermentation strains for the LSQ. After dual-strain co-fermentation, its pro-digestive enzymes and anti-inflammatory activities were significantly enhanced. Particularly, R. arrhizus/B. subtills fermentation group showed the prominent promotion of the enzymatic activities of amyloglucosidase, cellulase and trypsin, with AC200 values < 1.00 and Max fold increase values of 27.39 ± 0.22, 25.39 ± 0.87 and 48.07 ± 1.84, respectively, and anti-inflammatory activity with an IC50 value of 2.35 ± 0.18 mg/mL. Based on the correlation analysis of differential metabolic profiles and activities, the key pharmacodynamic metabolites were analyzed and validated, such as levomycetin succinatea, β-citrylglutamate, D-glucosaminic acid, nikkomycin and fucose 1-phosphate. Among them, D-glucosaminic acid was positively correlated with the promoting activity of amyloglucosidase, cellulose, enzyme trypsin, pepsin, and the inhibitory activity of NO production, and fucose 1-phosphate and nikkomycin had the prominently positive correlation with the promoting activity of pepsin (p < 0.01). In addition, the docking scores between them and digestive enzyme proteins were all < - 5. A new strategy involving the dual-strain fermentation of LSQ was investigated, and clarified the LSQ fermentation strain-constituent-pharmacological activity correlations, which provides a valuable reference for delving into the LSQ fermentation mechanism.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 3","pages":"52"},"PeriodicalIF":2.0,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0