Biotechnology Letters最新文献_第8页

Application of wood sawdust microbial transformation for the utilization of long-term stored bark-wood waste. 木屑微生物转化在长期贮存树皮木废弃物利用中的应用。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-04-10 DOI: 10.1007/s10529-026-03730-8

Ludmila Aleksandrovna Belovezhets, Dmitry Sergeevich Belousov, Nadezhda Vladimirovna Filinova, Ekaterina Olegovna Pristavka, Aleksey Aleksandrovich Pristavka

{"title":"Application of wood sawdust microbial transformation for the utilization of long-term stored bark-wood waste.","authors":"Ludmila Aleksandrovna Belovezhets, Dmitry Sergeevich Belousov, Nadezhda Vladimirovna Filinova, Ekaterina Olegovna Pristavka, Aleksey Aleksandrovich Pristavka","doi":"10.1007/s10529-026-03730-8","DOIUrl":"10.1007/s10529-026-03730-8","url":null,"abstract":"The issue of bark-wood waste (BWW) utilization is challenging for the regions adjacent to pulp and paper mills. Bark is difficult to recycle, and the additives used in paper production further complicate this process. This work focuses on the potential application of wood sawdust microbial transformation, the technology of which was developed at the Irkutsk Institute of Chemistry SB RAS, for the processing of bark-wood waste into environmentally safe soil. Laboratory experiments revealed that long-term stored BWW can undergo microbial degradation. The best results were obtained with the BWW/sawdust mixture. The substrate neutralization conditions were selected to simultaneously lower the pH and introduce the necessary mineral additives for microbial function. A semi-industrial experiment with a BWW/sawdust mixture of 20 m3 (3:1 ratio) was performed. The experiment demonstrated that pH stabilization occurred more efficiently than in the laboratory experiment. A temperature increase, which is characteristic of aerobic processing of lignocellulosic waste was also observed. The product obtained was non-toxic to both plants and Chlorella vulgaris (hazard class 5). The processing improved of the agrochemical parameters of the substrate, such as hydrolytic acidity, cation exchange capacity, and the sum of absorbed bases. The increase in humic acid content indicates a deep microbial degradation of the lignin component of BWW. Consequently, the microbial transformation of BWW was successfully implemented to produce a non-toxic soil. This technology can be scaled up for large volumes of BWW at storage sites.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147643537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Medical biotechnology and artificial intelligence powered companion diagnostics: indispensable pillars driving next generation precision health care. 医疗生物技术和人工智能辅助诊断：推动下一代精准医疗保健不可或缺的支柱。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-04-10 DOI: 10.1007/s10529-026-03732-6

Priyanka Sonar, K R Mathuvanti, Amoha Venkataramanan, Harish Reddy, Abhishek Mohanty

{"title":"Medical biotechnology and artificial intelligence powered companion diagnostics: indispensable pillars driving next generation precision health care.","authors":"Priyanka Sonar, K R Mathuvanti, Amoha Venkataramanan, Harish Reddy, Abhishek Mohanty","doi":"10.1007/s10529-026-03732-6","DOIUrl":"10.1007/s10529-026-03732-6","url":null,"abstract":"Medical biotechnology serves as a foundational platform for identifying disease markers and developing specific diagnostic assays. Complementing this, biosensors and medical devices translate these biomarkers into quantifiable signals enabling real-time health assessment. Artificial intelligence (AI) augments this framework by processing vast datasets to recognize patterns and generate predictive insights thereby aiding clinicians in decision-making and streamlining personalized disease management. The integration of molecular-based companion diagnostics, biosensor technology, and medical AI forms the cornerstone of contemporary, data-driven precision medicine. This review explores the synergistic convergence of these fields specifically examining how AI-based approaches enhance molecular diagnostics and biosensor devices to optimize clinical outcomes. This interdisciplinary collaboration facilitates advancing precision medicine. Consequently, these discovery technologies profoundly impact disease prevention, early diagnosis, and targeted therapeutic strategies. Despite challenges such as data privacy and technology accessibility, this cross-disciplinary approach holds tremendous potential for transforming the future of precision healthcare.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147643838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of Achromobacter sp. SM123 and immobilization of tributyl phosphate-degrading phosphatase enzyme for bioremediation application. SM123无色杆菌的分离及磷酸三丁酯降解磷酸酶的固定化。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-04-02 DOI: 10.1007/s10529-026-03726-4

Soumya Mukundan, Jose Savio Melo, Archana Mishra, Kuber Chandra Bhainsa

{"title":"Isolation of Achromobacter sp. SM123 and immobilization of tributyl phosphate-degrading phosphatase enzyme for bioremediation application.","authors":"Soumya Mukundan, Jose Savio Melo, Archana Mishra, Kuber Chandra Bhainsa","doi":"10.1007/s10529-026-03726-4","DOIUrl":"10.1007/s10529-026-03726-4","url":null,"abstract":"Tributyl phosphate (TBP) is a solvent and plasticizer commonly used in nuclear fuel reprocessing plants. However, it poses significant environmental challenges due to its stability, persistence, and unique physicochemical properties. To address this, the present study focuses on the biodegradation of TBP by an organism isolated from soil, capable of utilizing TBP as its sole carbon source. Isolated bacteria (Achromobacter sp. SM123) demonstrated efficient TBP degradation, achieving 96% degradation in 29 h following adaptation to butanol. The crude extract contains phosphatase enzyme, responsible for TBP degradation was immobilized onto hydroxyappetite matrix. Various immobilizing parameters were investigated, and storage stability study showed that the immobilized crude extract maintained 69% of its initial activity up to 65 days however the free enzyme retained only 27% activity over 23 days. The phosphatase activity present in crude extract was confirmed by zymogram analysis as a distinct purple band. This work highlights the potential application of isolated bacteria and its immobilized crude extract which contains phosphatase enzyme activity in the bioremediation of TBP-contaminated environment.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147607757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immobilized Aspergillus niger lipase over hydrogen titanate nanotubes as a biocatalyst for biodiesel production. 钛酸氢纳米管固定化黑曲霉脂肪酶作为生物柴油生产的生物催化剂。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-03-27 DOI: 10.1007/s10529-026-03710-y

Fatma Ahmed, Mai Raslan, A H Zaki

{"title":"Immobilized Aspergillus niger lipase over hydrogen titanate nanotubes as a biocatalyst for biodiesel production.","authors":"Fatma Ahmed, Mai Raslan, A H Zaki","doi":"10.1007/s10529-026-03710-y","DOIUrl":"10.1007/s10529-026-03710-y","url":null,"abstract":"Background: Immobilization of lipase enzyme is a promising approach towards cost-effective production of biodiesel to protect the enzyme from denaturation during the transesterification process.Methods: In the present study, hydrogen titanate nanotubes (HTNTs), synthesized by simple hydrothermal method, was used to immobilize lipase from Aspergillus niger via physical adsorption with the following ratios of HTNTs and lipase; 1 T: 1 L, 1 T: 0.75 L, 1 T: 0.5 L, and 1 T: 0.25 L. All prepared samples were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). The catalytic activities of free and immobilized lipases were evaluated for biodiesel production using sunflower oil, methanol to oil molar ratio of 4: 1 at 40 °C for 90 min.Results: The biodiesel yields (0% water content, 0.877 g/cm3 density, and 0.041 mg acid value) were 79.2 ± 0.01, 82.3 ± 0.9, 79.1 ± 1.2, 81.97 ± 1.4, 78 ± 0.1 percent for 1 T: 1 L, 1 T: 0.75 L, 1 T: 0.5 L, 1 T: 0.25 L, and free lipase (1 L), respectively. Compared with the control (1 L), immobilizing lipase using (HTNTs) enabled a 50- 75% reduction in lipase required quantity while maintaining or even increasing biodiesel production levels. The work establishes a promising method for lipase immobilization in biodiesel production that can be evaluated for large-scale application in further studies.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147526156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eco-friendly azo dye removal via a robust laccase from Acinetobacter baumannii KMSP005: a thermo-alkaliphilic biocatalytic approach. 利用鲍曼不动杆菌KMSP005的强力漆酶去除偶氮染料：一种热亲碱生物催化方法。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-03-26 DOI: 10.1007/s10529-026-03725-5

Kalyanee Bera, Mainak Mukhopadhyay

{"title":"Eco-friendly azo dye removal via a robust laccase from Acinetobacter baumannii KMSP005: a thermo-alkaliphilic biocatalytic approach.","authors":"Kalyanee Bera, Mainak Mukhopadhyay","doi":"10.1007/s10529-026-03725-5","DOIUrl":"10.1007/s10529-026-03725-5","url":null,"abstract":"The enzymatic breakdown of synthetic azo dyes is a potential and environmentally acceptable way to remove harmful contaminants commonly produced by the textile and dye industries. In this study, laccase-producing bacteria were isolated from soil samples collected from the Eastern Himalayan region and molecularly identified as Acinetobacter baumannii KMSP005 by 16S rDNA gene sequencing. The primary objective was to optimise laccase production using response surface methodology, while a secondary objective was to evaluate the efficiency of the bacterial laccase in degrading azo dye methyl red. Under optimised conditions, ~ 96% degradation of methyl red was achieved within 18 h, with a maximum laccase activity of 3.49 IU mL⁻1. Using coconut husk, a cheap agricultural waste, as a substrate for laccase production produced an impressive enzyme activity of 4.9 IU mL⁻1. When using agro-waste, laccase production increased by about 1.40-fold. Analysis of the breakdown products using gas chromatography-mass spectrometry revealed that methyl red was changed into simpler, less toxic, mediational compounds, proving the process' effectiveness and environmental safety. The maximal activity of 2.25 IU mL⁻1 at 50 °C and 2.50 IU mL⁻1 at pH 9 demonstrated the thermostable and alkali-tolerant characteristics of this laccase. The laccase exhibited thermo-alkaliphilic stability, retaining 82.05% of its initial activity at 50 °C and 86.66% activity at pH 9.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147519702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential of a Lactiplantibacillus plantarum strain to improve yield of tea tree seed oil through fermentation process. 一株植物乳杆菌发酵提高茶树籽油产量的潜力。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-03-25 DOI: 10.1007/s10529-026-03724-6

Peng-Ming Yang, Jin-Zhong Jiang

{"title":"The potential of a Lactiplantibacillus plantarum strain to improve yield of tea tree seed oil through fermentation process.","authors":"Peng-Ming Yang, Jin-Zhong Jiang","doi":"10.1007/s10529-026-03724-6","DOIUrl":"10.1007/s10529-026-03724-6","url":null,"abstract":"Great mass of tea tree seeds (TTS) are naturally rotted away as agricultural waste because there is no suitable technology used for extracting oil from TTS. The fermentation method is a new process and that can simultaneously recover oil, starch and saponins from TTS. In this study, a key bacterial strain named JJZ21 was isolated from fermented TTS milk (ground TTS and water) by the serial dilution method and identified as Lactiplantibacillus plantarum strain through molecular analyses. The production of cellulase, pectinase, amylase and protease of JJZ21 were visualized on agar plates containing specific enzyme substrates. Compared with the control, addition treatment with JJZ21 dramatically improved the activities of cellulase, pectinase, amylase and protease, reduced content of soluble sugar, protein and dry matter and pH in the TTS milk, shortened the fermentation time and increased the yield of TTS oil. Meanwhile, the addition of JJZ21 had no significant effect on quality of TTS oil. However, the yield of TTS oil decreased with excessive fermentation. Response surface methodology was used to evaluate the optimum conditions for fermentation process to obtain the maximum oil yield.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing the frontier of plant-based therapeutics: critical innovations in molecular farming and bioprocess Integration. 推进植物疗法的前沿：分子农业和生物过程整合的关键创新。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-03-23 DOI: 10.1007/s10529-026-03723-7

Kalidas Gowtham, Balamurugan Shanmugaraj, Lohith Saran Thangavel, Aksittha Srinivasan, Ashwini Malla

引用次数: 0

Stability of recombinant baculoviruses for biopharmaceutical applications in chemically defined medium. 生物制药用重组杆状病毒在化学定义介质中的稳定性。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-03-18 DOI: 10.1007/s10529-026-03720-w

Rodrigo Jorge Atanes Netto, Fernanda Angela Correia Barrence, Júlia Públio Rabello, Milena Miyu Teruya, Vanessa Farias, Eutimio Gustavo Fernández Núñez

{"title":"Stability of recombinant baculoviruses for biopharmaceutical applications in chemically defined medium.","authors":"Rodrigo Jorge Atanes Netto, Fernanda Angela Correia Barrence, Júlia Públio Rabello, Milena Miyu Teruya, Vanessa Farias, Eutimio Gustavo Fernández Núñez","doi":"10.1007/s10529-026-03720-w","DOIUrl":"10.1007/s10529-026-03720-w","url":null,"abstract":"Insect cell-baculovirus expression vector systems (IC-BEVS) are valuable tools in pharmaceutical bioprocesses for producing complex proteins like immunogenic virus-like particles. In this context, standardization is key to guarantee consistency in process yield, productivity, and product quality attributes. The study investigates the nine-month stability of the main biotechnological input used in IC-BEVS, the recombinant baculovirus vectors produced in chemically defined medium. Viral titer (pfu/mL), zeta potential (mV), and mean hydrodynamic particle size (μm), were employed to assess viral inactivation under eight combinations of generation and storage conditions. First-order, Weibull, and biphasic models were applied to describe viral decay. The critical parameters (factors) analyzed were the size of the heterologous gene inserted (719 and 1621 bp), storage temperature (- 80 and 1.5 °C), and infection time used for baculovirus batch generation (48 or 72 h post-infection). They were mainly explored according to a two-level factorial design (23). The primary quality attribute evaluated in this study was the one-log10 decay time of the viral titer (td), which exhibited an overall mean value of 80 days across eight batches. The biphasic model best fits the dispersion of the viral titer data collected over the assessed time in all considered combinations of factors and was employed to find significant factors over td values. Gene size was the only factor with a statistically significant effect on viral titer decay; additionally, the study indicates the occurrence of particle aggregation over the course of the analysis.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12999813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oligosaccharide oxidase for the enzymatic synthesis of glucosaminic acids. 低聚糖氧化酶，用于酶促合成氨基葡萄糖酸。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-03-17 DOI: 10.1007/s10529-026-03721-9

Olanrewaju Raji, Thu V Vuong, Nadia Davoudvandi, Emma R Master

引用次数: 0

Screening of a xylanase-producing Trichoderma strain and optimization of its enzyme production conditions. 产木聚糖酶木霉菌株筛选及产酶条件优化。

IF 2.5 4区生物学

Biotechnology Letters Pub Date : 2026-03-16 DOI: 10.1007/s10529-026-03718-4

Lili Fan, Kehe Fu, Jiameng Hu, Xin Liu

引用次数: 0