Biotechnology Letters最新文献_第10页

Neochloris oleoabundans cell wall rupture through melittin peptide: a new approach to increase lipid recovery 通过美利汀肽使油菜新孢子虫细胞壁破裂：提高脂质回收率的新方法

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-18 DOI: 10.1007/s10529-023-03451-2

Magda Vargas-Pérez, Azucena González-Horta, Hiram Mendoza-Hernández, Myriam Elías-Santos, Karim Acuña-Askar, Luis Jesús Galán-Wong, Hugo Alberto Luna-Olvera

{"title":"Neochloris oleoabundans cell wall rupture through melittin peptide: a new approach to increase lipid recovery","authors":"Magda Vargas-Pérez, Azucena González-Horta, Hiram Mendoza-Hernández, Myriam Elías-Santos, Karim Acuña-Askar, Luis Jesús Galán-Wong, Hugo Alberto Luna-Olvera","doi":"10.1007/s10529-023-03451-2","DOIUrl":"https://doi.org/10.1007/s10529-023-03451-2","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Objectives</h3>Microalgae cell wall affects the recovery of lipids, representing one of the main difficulties in the development of biofuel production. This work aimed to test a new method based on melittin peptide to induce a cellular disruption in N. oleoabundans.<h3 data-test=\"abstract-sub-heading\">Results</h3>Neochloris oleoabundans cells were grown at 32 °C in the presence of a high concentration of nitrate-phosphate, causing a cell disruption extent of 83.6%. Further, a two-fold increase in lipid recovery following melittin treatment and solvent extraction was observed. Additionally, it was possible to verify the effects of melittin, both before and after treatment on the morphology of the cells. Scanning electron microscopy (SEM) and confocal images of the melittin-treated microalgae revealed extensive cell damage with degradation of the cell wall and release of intracellular material. <h3 data-test=\"abstract-sub-heading\">Conclusions</h3>Melittin produced a selective cell wall rupture effect in N. oleoabundans under some culture conditions. These results represent the first report on the effect of melittin on lipid recovery from microalgae.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138717175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expansion of YALIcloneHR toolkit for Yarrowia lipolytica combined with Golden Gate and CRISPR technology 结合 "金门 "和 CRISPR 技术，扩展用于脂溶性亚罗威氏菌的 YALIcloneHR 工具包

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-08 DOI: 10.1007/s10529-023-03444-1

Qi Shen, Fang Yan, Ya-Wen Li, Jian Wang, Jia Ji, Wen-Xin Yan, Dan-Chen He, Ping Song, Tian-Qiong Shi

{"title":"Expansion of YALIcloneHR toolkit for Yarrowia lipolytica combined with Golden Gate and CRISPR technology","authors":"Qi Shen, Fang Yan, Ya-Wen Li, Jian Wang, Jia Ji, Wen-Xin Yan, Dan-Chen He, Ping Song, Tian-Qiong Shi","doi":"10.1007/s10529-023-03444-1","DOIUrl":"https://doi.org/10.1007/s10529-023-03444-1","url":null,"abstract":"Metabolic Engineering of yeast is a critical approach to improving the production capacity of cell factories. To obtain genetically stable recombinant strains, the exogenous DNA is preferred to be integrated into the genome. Previously, we developed a Golden Gate toolkit YALIcloneNHEJ, which could be used as an efficient modular cloning toolkit for the random integration of multigene pathways through the innate non-homologous end-joining repair mechanisms of Yarrowia lipolytica. We expanded the toolkit by designing additional building blocks of homologous arms and using CRISPR technology. The reconstructed toolkit was thus entitled YALIcloneHR and designed for gene-specific knockout and integration. To verify the effectiveness of the system, the gene PEX10 was selected as the target for the knockout. This system was subsequently applied for the arachidonic acid production, and the reconstructed strain can accumulate 4.8% of arachidonic acid. The toolkit will expand gene editing technology in Y. lipolytica, which would help produce other chemicals derived from acetyl-CoA in the future.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138552247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of d-glucaric acid with phosphoglucose isomerase-deficient Saccharomyces cerevisiae 利用磷酸葡萄糖异构酶缺陷的酿酒酵母生产 d-葡萄糖酸

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-08 DOI: 10.1007/s10529-023-03443-2

Mervi Toivari, Maija-Leena Vehkomäki, Laura Ruohonen, Merja Penttilä, Marilyn G. Wiebe

{"title":"Production of d-glucaric acid with phosphoglucose isomerase-deficient Saccharomyces cerevisiae","authors":"Mervi Toivari, Maija-Leena Vehkomäki, Laura Ruohonen, Merja Penttilä, Marilyn G. Wiebe","doi":"10.1007/s10529-023-03443-2","DOIUrl":"https://doi.org/10.1007/s10529-023-03443-2","url":null,"abstract":"d-Glucaric acid is a potential biobased platform chemical. Previously mainly Escherichia coli, but also the yeast Saccharomyces cerevisiae, and Pichia pastoris, have been engineered for conversion of d-glucose to d-glucaric acid via myo-inositol. One reason for low yields from the yeast strains is the strong flux towards glycolysis. Thus, to decrease the flux of d-glucose to biomass, and to increase d-glucaric acid yield, the four step d-glucaric acid pathway was introduced into a phosphoglucose isomerase deficient (Pgi1p-deficient) Saccharomyces cerevisiae strain. High d-glucose concentrations are toxic to the Pgi1p-deficient strains, so various feeding strategies and use of polymeric substrates were studied. Uniformly labelled 13C-glucose confirmed conversion of d-glucose to d-glucaric acid. In batch bioreactor cultures with pulsed d-fructose and ethanol provision 1.3 g d-glucaric acid L−1 was produced. The d-glucaric acid titer (0.71 g d-glucaric acid L−1) was lower in nitrogen limited conditions, but the yield, 0.23 g d-glucaric acid [g d-glucose consumed]−1, was among the highest that has so far been reported from yeast. Accumulation of myo-inositol indicated that myo-inositol oxygenase activity was limiting, and that there would be potential to even higher yield. The Pgi1p-deficiency in S. cerevisiae provides an approach that in combination with other reported modifications and bioprocess strategies would promote the development of high yield d-glucaric acid yeast strains.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138552336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kinetic and thermodynamic studies on the thermal inactivation of lipase immobilized on glutaraldehyde-activated rice husk silica 固定在戊二醛活化稻壳二氧化硅上的脂肪酶热失活的动力学和热力学研究

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-08 DOI: 10.1007/s10529-023-03449-w

Iara C. A. Bolina, Adriano A. Mendes

{"title":"Kinetic and thermodynamic studies on the thermal inactivation of lipase immobilized on glutaraldehyde-activated rice husk silica","authors":"Iara C. A. Bolina, Adriano A. Mendes","doi":"10.1007/s10529-023-03449-w","DOIUrl":"https://doi.org/10.1007/s10529-023-03449-w","url":null,"abstract":"The objective of this study was to obtain sufficient information on the thermal stabilization of a food-grade lipase from Thermomyces lanuginosus (TLL) using the immobilization technique. To do this, a new non-porous support was prepared via the sequential extraction of SiO2 from rice husks, followed by functionalization with (3-aminopropyl) triethoxysilane – 3-APTES (Amino–SiO2), and activation with glutaraldehyde – GA (GA-Amino-SiO2). We evaluated the influence of GA concentration, which varied from 0.25% v v−1 to 4% v v−1, on the immobilization parameters and enzyme thermal stabilization. The thermal inactivation parameters for both biocatalyst forms (soluble or immobilized TLL) were calculated by fitting a non-first-order enzyme inactivation kinetic model to the experimental data. According to the results, TLL was fully immobilized on the external support surface activated with different GA concentrations using an initial protein load of 5 mg g−1. A sharp decrease of hydrolytic activity was observed from 216.6 ± 12.4 U g−1 to 28.6 ± 0.9 U g−1 of after increasing the GA concentration from 0.25% v v−1 to 4.0% v v−1. The support that was prepared using a GA concentration at 0.5% v v−1 provided the highest stabilization of TLL – 31.6-times more stable than its soluble form at 60 °C. The estimations of the thermodynamic parameters, e.g., inactivation energy (Ed), enthalpy (ΔH#), entropy (ΔS#), and the Gibbs energy (ΔG#) values, confirmed the enzyme stabilization on the external support surface at temperatures ranging from 50 to 65 °C. These results show promising applications for this new heterogeneous biocatalyst in industrial processes given the high catalytic activity and thermal stability.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138552458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immobilization of rough morphotype Mycolicibacterium neoaurum R for androstadienedione production 固定粗糙形态的新牛磺酸霉菌 R 以生产雄甾二烯二酮

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-08 DOI: 10.1007/s10529-023-03448-x

Anqi Zhao, Yamei Li, Lixia Wu, Zhi Wang, Yongkun Lv, Wenlong Xiong, Mohammad Asraful Alam, Guohua Liu, Jingliang Xu

引用次数: 0

Enhancing suspended cell transfection by inducing localized distribution of the membrane actin cortex before exposure to electromechanical stimulation. 在暴露于机电刺激前，通过诱导肌动蛋白皮层膜的局部分布来增强悬浮细胞转染。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-01 Epub Date: 2023-09-01 DOI: 10.1007/s10529-023-03382-y

Wenjing Huang, Yibo Ma, Naotomo Tottori, Yoko Yamanishi

{"title":"Enhancing suspended cell transfection by inducing localized distribution of the membrane actin cortex before exposure to electromechanical stimulation.","authors":"Wenjing Huang, Yibo Ma, Naotomo Tottori, Yoko Yamanishi","doi":"10.1007/s10529-023-03382-y","DOIUrl":"10.1007/s10529-023-03382-y","url":null,"abstract":"Objectives: During physical transfection, an electrical field or mechanical force is used to induce cell transfection. We tested if the disruption of a dense actin layer underneath the membrane of a suspended cell enhances cell transfection.Results: A bubble generator was used to electromechanically stimulate suspended cells. To clarify the influence of the actin layer (the actin cortex) on cell transfection efficiency, we used an actin polymerization inhibitor (cytochalasin D) to disrupt the actin cortex before electromechanical stimulation. Without cytochalasin D treatment, signals from the overall actin cortex decreased after electromechanical stimulation. With cytochalasin D treatment, there was localized F-actin aggregation under static conditions. After electromechanical stimulation, there was a partial loss (localized disruption), but no overall disruption, of the actin cortex. With the pretreatment with cytochalasin D, the transfection efficiency of plasmids (4.7, 8.3, or 11 kbp) into NIH/3T3 or UMR-106 cells increased significantly after exposure to electromechanical stimulation.Conclusions: Localized distribution of the actin cortex before exposure to electromechanical stimulation is crucial for inducing a partial loss of the cortex, which improves transfection efficiency and large plasmid delivery.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10503042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, identification, and preparation of tyrosinase inhibitory peptides from Pinctada martensii meat. 马氏珠母贝肉酪氨酸酶抑制肽的分离、鉴定和制备。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-01 Epub Date: 2023-10-24 DOI: 10.1007/s10529-023-03437-0

Jinhao Meng, Jiaojiao Liu, Jing Lu, Pingyingzi Jiang, Yunxia Bai, Xiaoling Liu, Shubo Li

引用次数: 0

Unraveling the molecular mechanisms of selenite reduction: transcriptomic analysis of Bacillus reveals the key role of sulfur assimilation. 揭示亚硒酸盐还原的分子机制：芽孢杆菌的转录组学分析揭示了硫同化的关键作用。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-01 Epub Date: 2023-10-21 DOI: 10.1007/s10529-023-03439-y

Ying Yang, Jiawei Jing, Shuling Fan, Zhuo Chen, Yuanyuan Qu

{"title":"Unraveling the molecular mechanisms of selenite reduction: transcriptomic analysis of Bacillus reveals the key role of sulfur assimilation.","authors":"Ying Yang, Jiawei Jing, Shuling Fan, Zhuo Chen, Yuanyuan Qu","doi":"10.1007/s10529-023-03439-y","DOIUrl":"10.1007/s10529-023-03439-y","url":null,"abstract":"Selenite biotransformation by microorganisms is an effective detoxification and assimilation process. However, current knowledge of the molecular mechanisms of selenite reduction remains circumscribed. Here, the reduction of Se(IV) by a highly selenite-resistant Bacillus sp. SL (up to 50 mM) was systematically analyzed, and the molecular mechanisms of selenite reduction were investigated. Remarkably, 10 mM selenite was entirely transformed by the strain SL within 20 h, demonstrating a faster conversion rate compared to other microorganisms. Furthermore, glutathione (GSH) and exopolysaccharides (EPS) changes were also monitored during the process. Transcriptomic analysis revealed that the genes of ferredoxin-sulfite oxidoreductase (6.82) and sulfate adenylyltransferase (6.32) were significantly upregulated, indicating that the sulfur assimilation pathway is the primary reducing pathway involved in selenite reduction by strain SL. Moreover, key genes associated with NAD(P)/FAD-dependent oxidoreductases and thioredoxin were significantly upregulated. The reduction of Se(IV) was mediated by multiple pathways in strain SL. To our knowledge, this is the initial report to identify the involvement of sulfur assimilation pathway in selenite reduction for bacillus, which is rare in aerobic bacteria.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49673939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved preculture management for Cupriavidus necator cultivations. 改进了三角锥栽培的预培养管理。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-01 Epub Date: 2023-10-13 DOI: 10.1007/s10529-023-03436-1

Michelle-Sophie Gerlach, Peter Neubauer, Matthias Gimpel

{"title":"Improved preculture management for Cupriavidus necator cultivations.","authors":"Michelle-Sophie Gerlach, Peter Neubauer, Matthias Gimpel","doi":"10.1007/s10529-023-03436-1","DOIUrl":"10.1007/s10529-023-03436-1","url":null,"abstract":"Objectives: Research on hydrogenases from Cupriavidus necator has been ongoing for more than two decades and still today the common methods for culture inoculation are used. These methods were never adapted to the requirements of modified bacterial strains, resulting in different physiological states of the bacteria in the precultures, which in turn lead prolonged and different lag-phases.Results: In order to obtain uniform and always equally fit precultures for inoculation, we have established in this study an optimized protocol for precultures of the derivative of C. necator HF210 (C. necator HP80) which is used for homologous overexpression of the genes for the NAD+-reducing soluble hydrogenase (SH). We compared different media for preculture growth and determined the optimal time point for harvest. The protocol obtained in this study is based on two subsequent precultures, the first one in complex nutrient broth medium (NB) and a second one in fructose -nitrogen mineral salt medium (FN).Conclusion: Despite having two subsequent precultures our protocol reduces the preculture time to less than 30 h and provides reproducible precultures for cultivation of C. necator HP80.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10635987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41190054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromatographic purification of small extracellular vesicles using an affinity column for phospholipid membranes. 使用磷脂膜亲和柱对细胞外小泡进行色谱纯化。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-01 Epub Date: 2023-10-03 DOI: 10.1007/s10529-023-03430-7

Kanako Masaki, Abo Bakr F Ahmed, Takenori Ishida, Yuuki Mikami, Hisakage Funabashi, Ryuichi Hirota, Takeshi Ikeda, Akio Kuroda

{"title":"Chromatographic purification of small extracellular vesicles using an affinity column for phospholipid membranes.","authors":"Kanako Masaki, Abo Bakr F Ahmed, Takenori Ishida, Yuuki Mikami, Hisakage Funabashi, Ryuichi Hirota, Takeshi Ikeda, Akio Kuroda","doi":"10.1007/s10529-023-03430-7","DOIUrl":"10.1007/s10529-023-03430-7","url":null,"abstract":"Objectives: This study aimed to investigate whether chromatography using an ExoPUA column, an affinity column for phospholipid membranes, could potentially serve as an efficient, rapid, scalable, and reproducible method for purifying small extracellular vesicles (sEVs).Results: We used the ExoPUA column connected to a fast-performance liquid chromatography system. One-step chromatographic purification of sEVs from culture supernatant using the ExoPUA protocol resulted in an 82 ± 16-fold increase in purity with a yield of 38 ± 5% of sEVs. The purified sEVs contained CD9, CD63, TSG101, and miRNA (miR-21), but not the endoplasmic reticulum protein Calnexin. Transmission electron microscopy indicated that the purified sEVs were intact. The purification performance of the ExoPUA protocol showed superior results in terms of yield compared to that of the differential ultracentrifugation method, the most commonly used method for purifying sEVs in laboratories, and purity compared to that of the DEAE chromatography protocol.Conclusion: The sEVs were effectively purified in the bind-elute mode and the ExoPUA column can be refreshed and sterilized with sodium hydroxide (NaOH), having high potential for multiple sEV purification in a scalable and industrial manner.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10635940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41103367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0