Biotechnology Letters最新文献_第10页

Improved preculture management for Cupriavidus necator cultivations. 改进了三角锥栽培的预培养管理。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-01 Epub Date: 2023-10-13 DOI: 10.1007/s10529-023-03436-1

Michelle-Sophie Gerlach, Peter Neubauer, Matthias Gimpel

{"title":"Improved preculture management for Cupriavidus necator cultivations.","authors":"Michelle-Sophie Gerlach, Peter Neubauer, Matthias Gimpel","doi":"10.1007/s10529-023-03436-1","DOIUrl":"10.1007/s10529-023-03436-1","url":null,"abstract":"Objectives: Research on hydrogenases from Cupriavidus necator has been ongoing for more than two decades and still today the common methods for culture inoculation are used. These methods were never adapted to the requirements of modified bacterial strains, resulting in different physiological states of the bacteria in the precultures, which in turn lead prolonged and different lag-phases.Results: In order to obtain uniform and always equally fit precultures for inoculation, we have established in this study an optimized protocol for precultures of the derivative of C. necator HF210 (C. necator HP80) which is used for homologous overexpression of the genes for the NAD+-reducing soluble hydrogenase (SH). We compared different media for preculture growth and determined the optimal time point for harvest. The protocol obtained in this study is based on two subsequent precultures, the first one in complex nutrient broth medium (NB) and a second one in fructose -nitrogen mineral salt medium (FN).Conclusion: Despite having two subsequent precultures our protocol reduces the preculture time to less than 30 h and provides reproducible precultures for cultivation of C. necator HP80.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10635987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41190054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromatographic purification of small extracellular vesicles using an affinity column for phospholipid membranes. 使用磷脂膜亲和柱对细胞外小泡进行色谱纯化。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-01 Epub Date: 2023-10-03 DOI: 10.1007/s10529-023-03430-7

Kanako Masaki, Abo Bakr F Ahmed, Takenori Ishida, Yuuki Mikami, Hisakage Funabashi, Ryuichi Hirota, Takeshi Ikeda, Akio Kuroda

{"title":"Chromatographic purification of small extracellular vesicles using an affinity column for phospholipid membranes.","authors":"Kanako Masaki, Abo Bakr F Ahmed, Takenori Ishida, Yuuki Mikami, Hisakage Funabashi, Ryuichi Hirota, Takeshi Ikeda, Akio Kuroda","doi":"10.1007/s10529-023-03430-7","DOIUrl":"10.1007/s10529-023-03430-7","url":null,"abstract":"Objectives: This study aimed to investigate whether chromatography using an ExoPUA column, an affinity column for phospholipid membranes, could potentially serve as an efficient, rapid, scalable, and reproducible method for purifying small extracellular vesicles (sEVs).Results: We used the ExoPUA column connected to a fast-performance liquid chromatography system. One-step chromatographic purification of sEVs from culture supernatant using the ExoPUA protocol resulted in an 82 ± 16-fold increase in purity with a yield of 38 ± 5% of sEVs. The purified sEVs contained CD9, CD63, TSG101, and miRNA (miR-21), but not the endoplasmic reticulum protein Calnexin. Transmission electron microscopy indicated that the purified sEVs were intact. The purification performance of the ExoPUA protocol showed superior results in terms of yield compared to that of the differential ultracentrifugation method, the most commonly used method for purifying sEVs in laboratories, and purity compared to that of the DEAE chromatography protocol.Conclusion: The sEVs were effectively purified in the bind-elute mode and the ExoPUA column can be refreshed and sterilized with sodium hydroxide (NaOH), having high potential for multiple sEV purification in a scalable and industrial manner.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10635940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41103367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellulose-fueled microbial fuel cells equipped with a bipolar membrane using hydrogen phosphate as the final electron acceptor. 纤维素燃料微生物燃料电池配备双极膜，使用磷酸氢作为最终电子受体。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-01 Epub Date: 2023-10-03 DOI: 10.1007/s10529-023-03433-4

Iori Kazama, Naoto Hirose, Yuji Aso, Tomonari Tanaka, Hitomi Ohara

{"title":"Cellulose-fueled microbial fuel cells equipped with a bipolar membrane using hydrogen phosphate as the final electron acceptor.","authors":"Iori Kazama, Naoto Hirose, Yuji Aso, Tomonari Tanaka, Hitomi Ohara","doi":"10.1007/s10529-023-03433-4","DOIUrl":"10.1007/s10529-023-03433-4","url":null,"abstract":"Objectives: A bipolar membrane microbial fuel cell (bMFC) is used to generate electricity using cellulose in phosphate buffer solution as fuel, and the mechanism of electricity generation is elucidated from five reference experiments.Results: The bMFC was operated for 20 days using cellulose as fuel and Cellulomonas fimi. In the first reference experiment, no microorganism was used. In the second experiment, a cation-exchange membrane was used instead of a bipolar membrane. In the third experiment, the bipolar membrane was used in the opposite orientation as in the main experiment. In the fourth experiment, D2O was used instead of H2O in the cathode chamber. In the final experiment, the tris-maleate buffer was used instead of a phosphate buffer. Sufficient power generation did not occur in either reference experiment.Conclusions: The bMFC continuously generated electricity for 20 days, and elucidated H+ and OH- react in bipolar membrane, where the counter cation of dihydrogen phosphate served as the final electron acceptor.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41105436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishing a one-step marker-free CRISPR/Cas9 system for industrial Aspergillus niger using counter-selectable marker Ang-ace2. 使用反选择标记Ang-ace2建立工业黑曲霉的一步无标记CRISPR/Cas9系统。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-01 Epub Date: 2023-10-08 DOI: 10.1007/s10529-023-03434-3

Jiao Liu, Jie Zhu, Qian Zhang, Ruitong Lv, Hao Liu

引用次数: 0

Escherichia coli BL21(DE3) optimized deletion mutant as the host for whole-cell biotransformation of N‑acetyl‑D‑neuraminic acid. 大肠杆菌BL21(DE3)优化缺失突变体作为宿主进行N -乙酰- D -神经氨酸的全细胞生物转化。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-01 Epub Date: 2023-09-09 DOI: 10.1007/s10529-023-03426-3

Qiong Zhang, Jiao Zhang, Yanhong Shao, Guangdong Shang

{"title":"Escherichia coli BL21(DE3) optimized deletion mutant as the host for whole-cell biotransformation of N‑acetyl‑D‑neuraminic acid.","authors":"Qiong Zhang, Jiao Zhang, Yanhong Shao, Guangdong Shang","doi":"10.1007/s10529-023-03426-3","DOIUrl":"10.1007/s10529-023-03426-3","url":null,"abstract":"N‑Acetyl‑D‑neuraminic acid (Neu5Ac) is the crucial compound for the chemical synthesis of antiflu medicine Zanamivir. Chemoenzymatic synthesis of Neu5Ac involves N-acetyl-D-glucosamine 2-epimerase (AGE)-catalyzed epimerization of N-acetyl-D-glucosamine (GlcNAc) to N-acetyl-D-mannosamine (ManNAc), and aldolase-catalyzed condensation between ManNAc and pyruvate. Host optimization plays an important role in the whole-cell biotransformation of value-added compounds. In this study, via single-plasmid biotransformation system, we showed that the AGE gene BT0453, cloned from human gut microorganism Bacteroides thetaiotaomicron VPI-5482, showed the highest biotransformation yield among the AGE genes tested; and there is no clear Neu5Ac yield difference between the BT0453 coupled with one aldolase coding nanA gene and two nanA genes. Next, Escherichia coli chromosomal genes involved in substrate degradation, product exportation and pH change were deleted via recombineering and CRISPR/Cas9. With the final E. coli BL21(DE3) ΔnanA Δnag ΔpoxB as host, a significant 16.5% yield improvement was obtained. Furthermore, precursor (pyruvate) feeding resulted in 3.2% yield improvement, reaching 66.8% molar biotransformation. The result highlights the importance of host optimization, and set the stage for further metabolic engineering of whole-cell biotransformation of Neu5Ac.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10560284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Functional characterization of a cold related flavanone 3-hydroxylase from Tetrastigma hemsleyanum: an in vitro, in silico and in vivo study. 铁杉四柱头中一种冷相关黄烷酮3-羟化酶的功能表征：体外、计算机和体内研究。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-01 Epub Date: 2023-11-01 DOI: 10.1007/s10529-023-03440-5

Lishuang Wu, Jian Tian, Yao Yu, Lixia Yuan, Yujiong Zhang, Hao Wu, Furong Wang, Xin Peng

{"title":"Functional characterization of a cold related flavanone 3-hydroxylase from Tetrastigma hemsleyanum: an in vitro, in silico and in vivo study.","authors":"Lishuang Wu, Jian Tian, Yao Yu, Lixia Yuan, Yujiong Zhang, Hao Wu, Furong Wang, Xin Peng","doi":"10.1007/s10529-023-03440-5","DOIUrl":"10.1007/s10529-023-03440-5","url":null,"abstract":"Tetrastigma hemsleyanum Diels et Gilg, a traditional Chinese medicine, frequently suffers from cold damage in the winter, leading to lower yields. There is a pressing need to improve cold resistance; however, the mechanisms underlying T. hemsleyanum responses to cold stress are still not clearly understood. Here, we explored the function of the flavanone 3-hydroxylase gene (ThF3H) in T. hemsleyanum under cold treatment. The open reading frame of ThF3H is 1092 bp and encodes 363 amino acid residues. In vitro, the ThF3H enzyme was expressed in E. coli and successfully catalyzed naringenin and eriodictyol into dihydrokaempferol and dihydroquercetin, respectively. ThF3H exhibited a higher affinity for naringenin than for eriodictyol, which was in accordance with an in silico molecular docking analysis. The optimal pH and temperature for ThF3H activity were 7.0 and 30 °C, respectively. In vivo, overexpression of the ThF3H gene enhanced the cold tolerance of transgenic Arabidopsis lines, which was likely due to the increase in flavonoids. Collectively, the function of a cold-related ThF3H in the flavonoid biosynthesis pathway may be helpful for improving the cold tolerance of T. hemsleyanum through molecular breeding techniques.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71420363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of a xylose metabolic pathway in Trichosporonoides oedocephalis ATCC 16958 for the production of erythritol and xylitol. 在红头毛孢子虫ATCC 16958中构建木糖代谢途径，用于生产赤藓糖醇和木糖醇。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-01 Epub Date: 2023-10-13 DOI: 10.1007/s10529-023-03428-1

Zhou Deng, Yinghui Mu, Zhi Chen, Lishi Yan, Xin Ju, Liangzhi Li

{"title":"Construction of a xylose metabolic pathway in Trichosporonoides oedocephalis ATCC 16958 for the production of erythritol and xylitol.","authors":"Zhou Deng, Yinghui Mu, Zhi Chen, Lishi Yan, Xin Ju, Liangzhi Li","doi":"10.1007/s10529-023-03428-1","DOIUrl":"10.1007/s10529-023-03428-1","url":null,"abstract":"Purpose: Erythritol is a valuable compound as sweetener and chemical material however cannot be fermented from the abundant substrate xylose.Methods: The strain Trichosporonoides oedocephalis ATCC 16958 was employed to produce polyols including xylitol and erythritol by metabolic engineering approaches.Results: The introduction of a substrate-specific ribose-5-phosphate isomerase endowed T. oedocephalis with xylose-assimilation activity to produce xylitol, and eliminated glycerol production simultaneously. A more value-added product, erythritol was produced by further introducing a homologous xylulose kinase. The carbon flux was redirected from xylitol to erythritol by adding high osmotic pressure. The production of erythritol was improved to 46.5 g/L in flasks by fermentation adjustment, and the process was scaled up in a 5-L fermentor, with a 40 g/L erythritol production after 120 h, and a time-space yield of 0.56 g/L/h.Conclusion: This study demonstrated the potential of T. oedocephalis in the synthesis of multiple useful products from xylose.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41190053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic applications of carbon nanomaterials in renal cancer. 碳纳米材料在癌症治疗中的应用。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-01 Epub Date: 2023-10-21 DOI: 10.1007/s10529-023-03429-0

Jyotsna Priyam, Urmila Saxena

{"title":"Therapeutic applications of carbon nanomaterials in renal cancer.","authors":"Jyotsna Priyam, Urmila Saxena","doi":"10.1007/s10529-023-03429-0","DOIUrl":"10.1007/s10529-023-03429-0","url":null,"abstract":"Carbon nanomaterials (CNMs), including carbon nanotubes (CNTs), graphene, and nanodiamonds (NDs), have shown great promise in detecting and treating numerous cancers, including kidney cancer. CNMs can increase the sensitivity of diagnostic techniques for better kidney cancer identification and surveillance. They enable targeted medicine delivery specifically to tumour locations, with little effect on healthy tissue. Because of their unique chemical and physical characteristics, they can avoid the body's defence mechanisms, making it easier to accumulate where tumours exist. Consequently, CNMs provide more effective drug delivery to kidney cancer cells. It also helps in improving the efficacy of treatment. This review explores the potential of several CNMs in improving therapeutic strategies for kidney cancer. We briefly covered the physicochemical properties and therapeutic applications of CNMs. Additionally, we discussed how structural modifications in CNMs enhance their precision in treating renal cancer. A thorough overview of CNM-based gene, peptide, and drug delivery strategies for the treatment of renal cancer is presented in this review. It covers information on other CNM-based therapeutic approaches, such as hyperthermia, photodynamic therapy, and photoacoustic therapy. Also, the interactions of CNMs with the tumour microenvironment (TME) are explored, including modulation of the immune response, regulation of tumour hypoxia, interactions between CNMs and TME cells, effects of TME pH on CNMs, and more. Finally, potential side effects of CNMs, such as toxicity, bio corona formation, enzymatic degradation, and biocompatibility, are also discussed.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49673938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional regulation of NADPH oxidase in growth, microsclerotia formation and virulence in Metarhizium rileyi. NADPH氧化酶在绿僵菌生长、微孢子形成和毒力中的多功能调节。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-01 Epub Date: 2023-09-25 DOI: 10.1007/s10529-023-03427-2

Liqin Fan, Bingjie Li, Jiahui Wang, Xinxin Li, Feilong Ma, Fang Du, Hongli Li, Yunlong Lin

{"title":"Multifunctional regulation of NADPH oxidase in growth, microsclerotia formation and virulence in Metarhizium rileyi.","authors":"Liqin Fan, Bingjie Li, Jiahui Wang, Xinxin Li, Feilong Ma, Fang Du, Hongli Li, Yunlong Lin","doi":"10.1007/s10529-023-03427-2","DOIUrl":"10.1007/s10529-023-03427-2","url":null,"abstract":"Objectives: Microsclerotia (MS), anti-stress structures produced by many filamentous fungi, have been proven to be a great substitute for conidia in the production of insecticides within entomogenous fungi. NADPH oxidase (Nox) is a highly conserved ROS-response protein family that is widespread in eukaryotes and plays distinct roles in environmental fitness among various filamentous fungi. However, it is not clear whether the formation of MS and pathogenicity in entomogenous fungi is regulated by the Nox inside. In this study, we reported the presence of NADPH oxidase homologs in a great potential biocontrol fungus, Metarhizium rileyi, and further showed multiple biological functions.Results: Three Nox homologous genes in M. rileyi showed high expression throughout the entire process of MS formation. Targeted deletion of MrNoxA, MrNoxB and MrNoxR all led to a decrease in MS yield and impaired morphology. Moreover, the anti-adversity assay showed that they are indispensable for growth, osmotic pressure and oxidative stress regulation in Metarhizium rileyi. Most importantly, △MrNoxR and △MrNoxA but not △MrNoxB showed a dramatic reduction in virulence via inoculation. The normality of appressoria might be unaffected in mutants since there are no striking differences in virulence compared with WT by topical injections.Conclusion: Our results revealed that NADPH oxidase plays important roles in growth regulation, MS formation and pathogenicity in M. rileyi, perhaps in the ROS response and hyphal polarity.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41115057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofilm development of Candida boidinii and the effect of tyrosol on biofilm formation. 博伊迪尼假丝酵母生物膜的发育及酪醇对生物膜形成的影响。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-01 Epub Date: 2023-10-13 DOI: 10.1007/s10529-023-03432-5

Rita Márton, Blanka Nagy, Mónika Molnár

{"title":"Biofilm development of Candida boidinii and the effect of tyrosol on biofilm formation.","authors":"Rita Márton, Blanka Nagy, Mónika Molnár","doi":"10.1007/s10529-023-03432-5","DOIUrl":"10.1007/s10529-023-03432-5","url":null,"abstract":"Objectives: The applicability of a simple and high-throughput method for quantitative characterization of biofilm formation by Candida boidinii was tested in order to evaluate the effects of exogenous tyrosol on yeast growth and biofilm formation capacity.Results: Significant concentration-, temperature and time-dependent effect of tyrosol (2-(4-hydroxyphenyl)ethanol) was demonstrated, but it differentially affected the growth and biofilm formation (characterized by crystal violet staining and XTT-reduction assay) of Candida boidinii. Testing biofilm based on metabolic activity displayed sensitively the differences in the intensity of biofilm in terms of temperature, tyrosol concentration, and exposure time. At 22 °C after 24 h none of the tyrosol concentrations had significant effect, while at 30 °C tyrosol-mediated inhibition was observed at 50 mM and 100 mM concentration. After 48 h and 72 h at 22 °C, biofilm formation was stimulated at 6.25-25 mM concentrations, meanwhile at 30 °C tyrosol decreased the biofilm metabolic activity proportionally with the concentration.Conclusions: The research concludes that exogenous tyrosol exerts unusual effects on Candida boidinii growth and biofilm formation ability and predicts its potential application as a regulating factor of various fermentations by Candida boidinii.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10635961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41190052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0