Biotechnology Letters最新文献_第10页

Establishment of a semi-continuous scale-down clone screening model for intensified perfusion culture 为强化灌注培养建立半连续缩减克隆筛选模型

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-07-27 DOI: 10.1007/s10529-024-03512-0

Tao Sun, Yu Zhang, Hengrui Liang, Wenjing Fang, Zichen Qian, Kee Wee Tan, Junjie Li, Xiang Zheng, Mingyue Fang, Hang Zhou, Weichang Zhou, Sam Zhang

{"title":"Establishment of a semi-continuous scale-down clone screening model for intensified perfusion culture","authors":"Tao Sun, Yu Zhang, Hengrui Liang, Wenjing Fang, Zichen Qian, Kee Wee Tan, Junjie Li, Xiang Zheng, Mingyue Fang, Hang Zhou, Weichang Zhou, Sam Zhang","doi":"10.1007/s10529-024-03512-0","DOIUrl":"https://doi.org/10.1007/s10529-024-03512-0","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Purpose</h3>Perfusion cultures have been extensively used in the biotechnology industry to achieve high yields of recombinant products, especially those with stability issue. The WuXiUP™ platform represents a novel intensified perfusion that can achieve ultra‐high productivity. This study describes a representative scale-down 24-deep well plate (24-DWP) cell culture model for intensified perfusion clone screening.<h3 data-test=\"abstract-sub-heading\">Methods</h3>Clonal cell lines were expanded and evaluated in 24-DWP semi-continuous culture. Cell were sampled and counted daily with the aid of an automated liquid handler and high-throughput cell counter. To mimic perfusion culture, 24-DWP plates were spun down and resuspended with fresh medium daily. Top clones were ranked based on growth profiles and productivities. The best performing clones were evaluated on bioreactors.<h3 data-test=\"abstract-sub-heading\">Results</h3>The selected clones achieved volumetric productivity (Pv) up to 5 g/L/day when expressing a monoclonal antibody, with the accumulative harvest Pv exceeding 60 g/L in a 21-day cell culture. Product quality attributes of clones cultured in 24-DWP were comparable with those from bioreactors. A high seeding strategy further shortened the clone screening timeline.<h3 data-test=\"abstract-sub-heading\">Conclusion</h3>In this study, a 24-DWP semi-continuous scale-down model was successfully developed to screen for cell lines suitable for intensified perfusion culture.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"70 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141783586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Native CRISPR-Cas-based programmable multiplex gene repression in Klebsiella variicola 变异克雷伯氏菌中基于 CRISPR-Cas 的原生可编程多重基因抑制

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-07-27 DOI: 10.1007/s10529-024-03516-w

Zhifeng Mo, Siying Lin, Ting Li, Guohui Yu, Yunhao Sun, Jianuan Zhou, Zeling Xu

{"title":"Native CRISPR-Cas-based programmable multiplex gene repression in Klebsiella variicola","authors":"Zhifeng Mo, Siying Lin, Ting Li, Guohui Yu, Yunhao Sun, Jianuan Zhou, Zeling Xu","doi":"10.1007/s10529-024-03516-w","DOIUrl":"https://doi.org/10.1007/s10529-024-03516-w","url":null,"abstract":"Klebsiella variicola is a Gram-negative bacterium that is frequently isolated from a wide variety of natural niches. It is a ubiquitous opportunistic pathogen that can cause diverse infections in plants, animals, and humans. It also has significant biotechnological potential. However, due to the lack of efficient genetic tools, the molecular basis contributing to the pathogenesis and beneficial activities of K. variicola remains poorly understood. In this study, we found and characterized a native type I-E CRISPR-Cas system in a recently isolated K. variicola strain KV-1. The system cannot cleave target DNA sequences due to the inactivation of the Cas3 nuclease by a transposable element but retains the activity of the crRNA-guided Cascade binding to the target DNA sequence. A targeting plasmid carrying a mini-CRISPR to encode a crRNA was designed and introduced into the KV-1 strain, which successfully repurposed the native type I-E CRISPR-Cas system to inhibit the expression of the target gene efficiently and specifically. Moreover, by creating a mini-CRISPR to encode multiple crRNAs, multiplex gene repression was achieved by providing a single targeting plasmid. This work provides the first native CRISPR-Cas-based tool for programmable multiplex gene repression in K. variicola, which will facilitate studying the pathogenic mechanism of K. variicola and enable metabolic engineering to produce valuable bioproducts.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141786056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Redifferentiation of genetically modified dedifferentiated chondrocytes in a microcavitary hydrogel. 转基因再分化软骨细胞在微腔水凝胶中的再分化。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-06-01 Epub Date: 2024-03-25 DOI: 10.1007/s10529-024-03475-2

Yongchang Yao, Ke Chen, Qian Pan, Hui Gao, Weixian Su, Shicong Zheng, Weiqiang Dong, Dongyang Qian

{"title":"Redifferentiation of genetically modified dedifferentiated chondrocytes in a microcavitary hydrogel.","authors":"Yongchang Yao, Ke Chen, Qian Pan, Hui Gao, Weixian Su, Shicong Zheng, Weiqiang Dong, Dongyang Qian","doi":"10.1007/s10529-024-03475-2","DOIUrl":"10.1007/s10529-024-03475-2","url":null,"abstract":"Objectives: We genetically modified dedifferentiated chondrocytes (DCs) using lentiviral vectors and adenoviral vectors encoding TGF-β3 (referred to as transgenic groups below) and encapsulated these DCs in the microcavitary hydrogel and investigated the combinational effect on redifferentiation of the genetically manipulated DCs.Results: The Cell Counting Kit-8 data indicated that both transgenic groups exhibited significantly higher cell viability in the first week but inferior cell viability in the subsequent timepoints compared with those of the control group. Real-time polymerase chain reaction and western blot analysis results demonstrated that both transgenic groups had a better effect on redifferentiation to some extent, as evidenced by higher expression levels of chondrogenic genes, suggesting the validity of combination with transgenic DCs and the microcavitary hydrogel on redifferentiation. Although transgenic DCs with adenoviral vectors presented a superior extent of redifferentiation, they also expressed greater levels of the hypertrophic gene type X collagen. It is still worth further exploring how to deliver TGF-β3 more efficiently and optimizing the appropriate parameters, including concentration and duration.Conclusions: The results demonstrated the better redifferentiation effect of DCs with the combinational use of transgenic TGF-β3 and a microcavitary alginate hydrogel and implied that DCs would be alternative seed cells for cartilage tissue engineering due to their easily achieved sufficient cell amounts through multiple passages and great potential to redifferentiate to produce cartilaginous extracellular matrix.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"483-495"},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140206322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accumulation of docosapentaenoic acid (n-3 DPA) in a novel isolate of the marine ichthyosporean Sphaeroforma arctica. 在海洋鱼类 Sphaeroforma arctica 的新分离物中积累二十二碳五烯酸（n-3 DPA）。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-06-01 Epub Date: 2024-03-16 DOI: 10.1007/s10529-024-03472-5

Qiang Wilson Yan, Ying-Chun Liu, Christa Barrett, Kelly Haake, Daniel Seeler, Oliver May, Ross Zirkle

{"title":"Accumulation of docosapentaenoic acid (n-3 DPA) in a novel isolate of the marine ichthyosporean Sphaeroforma arctica.","authors":"Qiang Wilson Yan, Ying-Chun Liu, Christa Barrett, Kelly Haake, Daniel Seeler, Oliver May, Ross Zirkle","doi":"10.1007/s10529-024-03472-5","DOIUrl":"10.1007/s10529-024-03472-5","url":null,"abstract":"Objective: Currently, there is lack of a consistent and highly enriched source for docosapentaenoic acid (n-3 DPA, C22:5), and this work report the isolation of microorganism that naturally produces n-3 DPA.Results: In this work, we screened microorganisms in our culture collections with the goal to isolate a strain with high levels of n-3 DPA. We isolated a strain of Sphaeroforma arctica that produces up to 11% n-3 DPA in total fatty acid and has a high n-3 DPA to DHA/EPA ratio. The cell growth of the isolated strain was characterized using microscopy imaging and flow cytometer technologies to confirm the coenocytic pattern of cell divisions previously described in S. arctica. Our novel isolate of S. arctica grew more robustly and produced significantly more n-3 DPA compared to previously isolated and described strains indicating the uniqueness of the discovered strain.Conclusion: Overall, this work reports a first isolate n-3 DPA producing microorganism and establishes the foundation for future strain improvement and elucidation of the physiological function of this LC-PUFA for human nutrition and health.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"373-383"},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140139816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A recombinant fungal photolyase autonomously enters human cell nuclei to fix UV-induced DNA lesions. 重组真菌光解酶可自主进入人类细胞核，修复紫外线诱导的 DNA 损伤。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-06-01 Epub Date: 2024-03-25 DOI: 10.1007/s10529-024-03474-3

Yuting Bao, Weiguo Fang

{"title":"A recombinant fungal photolyase autonomously enters human cell nuclei to fix UV-induced DNA lesions.","authors":"Yuting Bao, Weiguo Fang","doi":"10.1007/s10529-024-03474-3","DOIUrl":"10.1007/s10529-024-03474-3","url":null,"abstract":"Solar ultraviolet radiations induced DNA damages in human skin cells with cyclobutane pyrimidine dimers (CPD) and (6-4) photoproducts (6-4PPs) as the most frequent lesions. CPDs are repaired much slower than 6-4PPs by the nucleotide excision repair pathway, which are thus the major lesions that interfere with key cellular processes and give rise to gene mutations, possibly resulting in skin cancer. In prokaryotes and multicellular eukaryotes other than placental mammals, CPDs can be rapidly repaired by CPD photolyases in one simple enzymatic reaction using the energy of blue light. In this study, we aim to construct recombinant CPD photolyases that can autonomously enter human cell nuclei to fix UV-induced CPDs. A fly cell penetration peptide and a viral nucleus localization signal peptide were recombined with a fungal CPD photolyase to construct a recombinant protein. This engineered CPD photolyase autonomously crosses cytoplasm and nuclear membrane of human cell nuclei, which then efficiently photo-repairs UV-induced CPD lesions in the genomic DNA. This further protects the cells by increasing SOD activity, and decreasing cellular ROSs, malondialdehyde and apoptosis.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"459-467"},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140206320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ANCUT1, a novel thermoalkaline cutinase from Aspergillus nidulans and its application on hydroxycinnamic acids lipophilization. 来自裸曲霉的新型热碱性角叉菜酶 ANCUT1 及其在羟基肉桂酸脂化中的应用

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-06-01 Epub Date: 2024-02-28 DOI: 10.1007/s10529-024-03467-2

Carolina Peña-Montes, Eva Bermúdez-García, Denise Castro-Ochoa, Fernanda Vega-Pérez, Katia Esqueda-Domínguez, José Augusto Castro-Rodríguez, Augusto González-Canto, Laura Segoviano-Reyes, Arturo Navarro-Ocaña, Amelia Farrés

{"title":"ANCUT1, a novel thermoalkaline cutinase from Aspergillus nidulans and its application on hydroxycinnamic acids lipophilization.","authors":"Carolina Peña-Montes, Eva Bermúdez-García, Denise Castro-Ochoa, Fernanda Vega-Pérez, Katia Esqueda-Domínguez, José Augusto Castro-Rodríguez, Augusto González-Canto, Laura Segoviano-Reyes, Arturo Navarro-Ocaña, Amelia Farrés","doi":"10.1007/s10529-024-03467-2","DOIUrl":"10.1007/s10529-024-03467-2","url":null,"abstract":"One of the four cutinases encoded in the Aspergillus nidulans genome, ANCUT1, is described here. Culture conditions were evaluated, and it was found that this enzyme is produced only when cutin is present in the culture medium, unlike the previously described ANCUT2, with which it shares 62% amino acid identity. The differences between them include the fact that ANCUT1 is a smaller enzyme, with experimental molecular weight and pI values of 22 kDa and 6, respectively. It shows maximum activity at pH 9 and 60 °C under assayed conditions and retains more than 60% of activity after incubation for 1 h at 60 °C in a wide range of pH values (6-10) after incubations of 1 or 3 h. It has a higher activity towards medium-chain esters and can modify long-chain length hydroxylated fatty acids constituting cutin. Its substrate specificity properties allow the lipophilization of alkyl coumarates, valuable antioxidants and its thermoalkaline behavior, which competes favorably with other fungal cutinases, suggests it may be useful in many more applications.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"409-430"},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11055803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139982262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recycling selectable markers via Cre/loxP system for constructing Komagataella phaffii strains co-expressing multiple proteins. 通过 Cre/loxP 系统回收可选择标记，构建共同表达多种蛋白质的 Komagataella phaffii 菌株。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-06-01 Epub Date: 2024-02-28 DOI: 10.1007/s10529-024-03466-3

Weixian Wang, Minghai Han, Guofei Zhu, Xiaohui Liu, Tianming Zhao, Xiaoyan Ma, Xun Gong, Cunbin Xu

{"title":"Recycling selectable markers via Cre/loxP system for constructing Komagataella phaffii strains co-expressing multiple proteins.","authors":"Weixian Wang, Minghai Han, Guofei Zhu, Xiaohui Liu, Tianming Zhao, Xiaoyan Ma, Xun Gong, Cunbin Xu","doi":"10.1007/s10529-024-03466-3","DOIUrl":"10.1007/s10529-024-03466-3","url":null,"abstract":"Objective: A convenient strategy was developed to recycle selectable markers using Cre/loxP system for constructing Komagataella phaffii strains co-expressing multiple proteins.Results: A plasmid in this strategy was generated from pPICZαA with integration of lox71-Sh ble-lox66. Firstly, the plasmid was inserted with one target protein gene and then transformed into K. phaffii KM71. Secondly, the auxiliary plasmid pPICZαA/cre/his4 containing CRE recombinase gene was further chromosomally inserted to Sh ble gene therein. Finally, methanol induction was conducted to produce CRE for Cre/loxP-mediated recombination, and consequently, the sequence between lox71 and lox66 was deleted, leading to recycling of ZeoR and His- markers. Then the resulted strain expressing the one target protein was used as the host to which another target protein gene could be inserted by the same procedures.Conclusions: With easy manipulation, the method was effective in recycling of the selectable markers, and consequently two protein genes were sequential integrated chromosomally and successfully co-expressed in the yeast.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"399-407"},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139982263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computer-aided rational design strategy based on protein surface charge to improve the thermal stability of a novel esterase from Geobacillus jurassicus. 基于蛋白质表面电荷的计算机辅助合理设计策略，提高侏罗纪地杆菌新型酯酶的热稳定性。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-06-01 Epub Date: 2024-03-25 DOI: 10.1007/s10529-024-03473-4

Runfei Song, Jin Zhang, Mengyu Zhu, Lin Lin, Wei Wei, Dongzhi Wei

{"title":"Computer-aided rational design strategy based on protein surface charge to improve the thermal stability of a novel esterase from Geobacillus jurassicus.","authors":"Runfei Song, Jin Zhang, Mengyu Zhu, Lin Lin, Wei Wei, Dongzhi Wei","doi":"10.1007/s10529-024-03473-4","DOIUrl":"10.1007/s10529-024-03473-4","url":null,"abstract":"Objectives: Although Geobacillus are significant thermophilic bacteria source, there are no reports of thermostable esterase gene in Geobacillus jurassicus or rational design strategies to increase the thermal stability of esterases.Results: Gene gju768 showed a highest similarity of 15.20% to esterases from Geobacillus sp. with detail enzymatic properties. Using a combination of Gibbs Unfolding Free Energy (∆∆G) calculator and the distance from the mutation site to the catalytic site (DsCα-Cα) to screen suitable mutation sites with elimination of negative surface charge, the mutants (D24N, E221Q, and E253Q) displayed stable mutants with higher thermal stability than the wild-type (WT). Mutant E253Q exhibited the best thermal stability, with a half-life (T1/2) at 65 °C of 32.4 min, which was 1.8-fold of the WT (17.9 min).Conclusion: Cloning of gene gju768 and rational design based on surface charge engineering contributed to the identification of thermostable esterase from Geobacillus sp. and the exploration of evolutionary strategies for thermal stability.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"443-458"},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140206321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silica nanoparticles enhance interfacial self-adherence of a multi-layered extracellular matrix scaffold for vascular tissue regeneration. 二氧化硅纳米粒子增强多层细胞外基质支架的界面自粘附性，促进血管组织再生。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-06-01 Epub Date: 2024-02-17 DOI: 10.1007/s10529-024-03469-0

Leslie A Goldberg, Helena D Zomer, Calum McFetridge, Peter S McFetridge

{"title":"Silica nanoparticles enhance interfacial self-adherence of a multi-layered extracellular matrix scaffold for vascular tissue regeneration.","authors":"Leslie A Goldberg, Helena D Zomer, Calum McFetridge, Peter S McFetridge","doi":"10.1007/s10529-024-03469-0","DOIUrl":"10.1007/s10529-024-03469-0","url":null,"abstract":"Purpose: Based on the clinical need for grafts for vascular tissue regeneration, our group developed a customizable scaffold derived from the human amniotic membrane. Our approach consists of rolling the decellularized amniotic membrane around a mandrel to form a multilayered tubular scaffold with tunable diameter and wall thickness. Herein, we aimed to investigate if silica nanoparticles (SiNP) could enhance the adhesion of the amnion layers within these rolled grafts.Methods: To test this, we assessed the structural integrity and mechanical properties of SiNP-treated scaffolds. Mechanical tests were repeated after six months to evaluate adhesion stability in aqueous environments.Results: Our results showed that the rolled SiNP-treated scaffolds maintained their tubular shape upon hydration, while non-treated scaffolds collapsed. By scanning electron microscopy, SiNP-treated scaffolds presented more densely packed layers than untreated controls. Mechanical analysis showed that SiNP treatment increased the scaffold's tensile strength up to tenfold in relation to non-treated controls and changed the mechanism of failure from interfacial slipping to single-point fracture. The nanoparticles reinforced the scaffolds both at the interface between two distinct layers and within each layer of the extracellular matrix. Finally, SiNP-treated scaffolds significantly increased the suture pullout force in comparison to untreated controls.Conclusion: Our study demonstrated that SiNP prevents the unraveling of a multilayered extracellular matrix graft while improving the scaffolds' overall mechanical properties. In addition to the generation of a robust biomaterial for vascular tissue regeneration, this novel layering technology is a promising strategy for a number of bioengineering applications.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"469-481"},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139897774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotechnological potential of red yeast isolated from birch forests in Poland 从波兰桦树林中分离的红酵母的生物技术潜力

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-04-30 DOI: 10.1007/s10529-024-03482-3

Anna M. Kot, Paulina Laszek, Marek Kieliszek, Katarzyna Pobiega, Stanisław Błażejak

{"title":"Biotechnological potential of red yeast isolated from birch forests in Poland","authors":"Anna M. Kot, Paulina Laszek, Marek Kieliszek, Katarzyna Pobiega, Stanisław Błażejak","doi":"10.1007/s10529-024-03482-3","DOIUrl":"https://doi.org/10.1007/s10529-024-03482-3","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Objectives</h3>This study aimed to isolate red yeast from sap, bark and slime exudates collected from Polish birch forests and then assessment of their biotechnological potential.<h3 data-test=\"abstract-sub-heading\">Results</h3>24 strains of red yeast were isolated from the bark, sap and spring slime fluxes of birch (Betula pendula). Strains belonging to Rhodotorula mucilaginosa (6), Rhodosporidiobolus colostri (4), Cystrofilobasidium capitaum (3), Phaffia rhodozyma (3) and Cystobasidium psychroaquaticum (3) were dominant. The highest efficiency of carotenoid biosynthesis (5.04 mg L−1) was obtained by R. mucilaginosa CMIFS 004, while lipids were most efficiently produced by two strains of P. rhodozyma (5.40 and 5.33 g L−1). The highest amount of exopolysaccharides (3.75 g L−1) was produced by the R. glutinis CMIFS 103. Eleven strains showed lipolytic activity, nine amylolytic activity, and only two proteolytic activity. The presence of biosurfactants was not found. The growth of most species of pathogenic moulds was best inhibited by Rhodotorula yeasts.<h3 data-test=\"abstract-sub-heading\">Conclusion</h3>Silver birch is a good natural source for the isolation of new strains of red yeast with wide biotechnological potential.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"41 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140833645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0