Biotechnology Letters最新文献_第10页

Denovo production of resveratrol by engineered Saccharomyces cerevisiae W303-1a using pretreated Gracilaria corticata extracts. 利用预处理的藤草提取物，利用工程酿酒酵母W303-1a生产白藜芦醇。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-02-01 Epub Date: 2023-11-21 DOI: 10.1007/s10529-023-03441-4

Nishanthika Thenmozhi Kulasekaran, Mary Leema Thilakam, Dharani Gopal, Jung-Kul Lee, Jeya Marimuthu

{"title":"Denovo production of resveratrol by engineered Saccharomyces cerevisiae W303-1a using pretreated Gracilaria corticata extracts.","authors":"Nishanthika Thenmozhi Kulasekaran, Mary Leema Thilakam, Dharani Gopal, Jung-Kul Lee, Jeya Marimuthu","doi":"10.1007/s10529-023-03441-4","DOIUrl":"10.1007/s10529-023-03441-4","url":null,"abstract":"Objective: Assembly and construction of resveratrol production pathway in Saccharomyces cerevisiae for denovo production of resveratrol using seaweed extract as fermentation medium.Results: Genes involved in the production of resveratrol from tyrosine pathway, tyrosine ammonia lyase (FTAL) gene from Flavobacterium johnsoniae (FjTAL), the 4-coumarate:CoA ligase gene from Arabidopsis thaliana (4CL1) and the stilbene synthase gene from Vitis vinifera (VvSTS) were introduced into low copy, high copy and integrative vector and transformed into S. cerevisiae W303-1a. The resulting strains W303-1a/pARS-res5, W303-1a/2µ-res1 and W303-1a/IntUra-res9 produced a level of 2.39 ± 0.01, 3.33 ± 0.03 and 8.34 ± 0.03 mg resveratrol l-1 respectively. CRISPR mediated integration at the δ locus resulted in 17.13 ± 1.1 mg resveratrol l-1. Gracilaria corticata extract was tested as a substrate for the growth of transformant to produce resveratrol. The strain produced a comparable level, 13.6 ± 0.54 mg resveratrol l-1 when grown in seaweed extract medium.Conclusions: The strain W303-1a/IntδC-res1 utilized Gracillaria hydrolysate and produced 13.6 ± 0.54 mg resveratrol l-1 and further investigations are being carried out focusing on pathway engineering and optimization of process parameters to enhance resveratrol yield.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"19-28"},"PeriodicalIF":2.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138175511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of T16H subcellular localization for promoting its catalytic efficiency in yeast cells. 调控T16H亚细胞定位促进其在酵母细胞中的催化效率。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2024-02-01 Epub Date: 2023-11-16 DOI: 10.1007/s10529-023-03442-3

Jiancong Cao, Bingrun Yang, Mengxia Zhang, Fang Yu

{"title":"Regulation of T16H subcellular localization for promoting its catalytic efficiency in yeast cells.","authors":"Jiancong Cao, Bingrun Yang, Mengxia Zhang, Fang Yu","doi":"10.1007/s10529-023-03442-3","DOIUrl":"10.1007/s10529-023-03442-3","url":null,"abstract":"To investigate the effect of subcellular localization on the transformation efficiency of heterologous expressed functional P450s in yeast. Microbial biotransformation offers a promising substitute for the direct extraction of natural products, but its viability in industrial applications depends on achieving high transformation efficiencies. To investigate the influence of subcellular microenvironments on the activity of heterologously expressed P450s, Catharanthus roseus tabersonine 16-hydroxylase (T16H) was chosen, and its subcellular localization was regulated by fusing organelle-localization signals. Interestingly, this manipulation had no effect on the gene expression levels of T16H, but resulted in varying conversion rates from tabersonine to 16-hydroxy tabersonine. Notably, the highest transformation efficiency was observed in yeast cells expressing peroxisome-localized T16H. Given the alkaline pH optimum for P450s, the alkaline peroxisomal lumen could be a suitable compartment for P450s reactions to achieve high transformation efficiency using yeast cells. Different organelle-localization of T16H in yeast cells resulted in varying conversion rates, suggesting that compartmentalizing the expression of target enzymes could be a viable approach to increase transformation efficiency in yeast.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"29-35"},"PeriodicalIF":2.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136396076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of cocoonase and cocoonase like protein using polyclonal antibody of Antheraea mylitta cocoonase 使用多克隆抗体鉴定蚁蚕茧酶和类茧酶蛋白

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-18 DOI: 10.1007/s10529-023-03447-y

Aruna Rani, Dev Mani Pandey, Jay Prakash Pandey

{"title":"Identification of cocoonase and cocoonase like protein using polyclonal antibody of Antheraea mylitta cocoonase","authors":"Aruna Rani, Dev Mani Pandey, Jay Prakash Pandey","doi":"10.1007/s10529-023-03447-y","DOIUrl":"https://doi.org/10.1007/s10529-023-03447-y","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Background</h3>Cocoonase is a proteolytic enzyme released by silk moths during pupal adult emergence. Without damaging the silk fibroin, this enzyme dissolves the shell of the tasar cocoon by exclusively targeting the protein sericin. Prior to this study, there was no available antibody against Antheraea mylitta cocoonase to identify or screen out similar variants or cocoonase like protein.<h3 data-test=\"abstract-sub-heading\">Results</h3>In the present study, naturally secreted A. mylitta cocoonase was purified and used to immunize New Zealand white rabbits. The developed polyclonal antibody of cocoonase was purified and its specific interaction with cocoonase was determined using Indirect ELISA. The confirmation of its specificity and immuno-reactivity was evaluated by western blot using native cocoonase of tasar silkworm A. mylitta. The efficacy and specificity of the polyclonal antibody were further verified and confirmed by western blot which was performed to detect ten different ecotypes of A. mylitta cocoonase.<h3 data-test=\"abstract-sub-heading\">Conclusion</h3>The developed antibody successfully detected the cocoonase of different ecotypes. Thus, in future this antibody can serve as one of the molecular detection method for cocoonase and cocoonase-like proteins.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"32 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138717211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neochloris oleoabundans cell wall rupture through melittin peptide: a new approach to increase lipid recovery 通过美利汀肽使油菜新孢子虫细胞壁破裂：提高脂质回收率的新方法

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-18 DOI: 10.1007/s10529-023-03451-2

Magda Vargas-Pérez, Azucena González-Horta, Hiram Mendoza-Hernández, Myriam Elías-Santos, Karim Acuña-Askar, Luis Jesús Galán-Wong, Hugo Alberto Luna-Olvera

{"title":"Neochloris oleoabundans cell wall rupture through melittin peptide: a new approach to increase lipid recovery","authors":"Magda Vargas-Pérez, Azucena González-Horta, Hiram Mendoza-Hernández, Myriam Elías-Santos, Karim Acuña-Askar, Luis Jesús Galán-Wong, Hugo Alberto Luna-Olvera","doi":"10.1007/s10529-023-03451-2","DOIUrl":"https://doi.org/10.1007/s10529-023-03451-2","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Objectives</h3>Microalgae cell wall affects the recovery of lipids, representing one of the main difficulties in the development of biofuel production. This work aimed to test a new method based on melittin peptide to induce a cellular disruption in N. oleoabundans.<h3 data-test=\"abstract-sub-heading\">Results</h3>Neochloris oleoabundans cells were grown at 32 °C in the presence of a high concentration of nitrate-phosphate, causing a cell disruption extent of 83.6%. Further, a two-fold increase in lipid recovery following melittin treatment and solvent extraction was observed. Additionally, it was possible to verify the effects of melittin, both before and after treatment on the morphology of the cells. Scanning electron microscopy (SEM) and confocal images of the melittin-treated microalgae revealed extensive cell damage with degradation of the cell wall and release of intracellular material. <h3 data-test=\"abstract-sub-heading\">Conclusions</h3>Melittin produced a selective cell wall rupture effect in N. oleoabundans under some culture conditions. These results represent the first report on the effect of melittin on lipid recovery from microalgae.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"55 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138717175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expansion of YALIcloneHR toolkit for Yarrowia lipolytica combined with Golden Gate and CRISPR technology 结合 "金门 "和 CRISPR 技术，扩展用于脂溶性亚罗威氏菌的 YALIcloneHR 工具包

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-08 DOI: 10.1007/s10529-023-03444-1

Qi Shen, Fang Yan, Ya-Wen Li, Jian Wang, Jia Ji, Wen-Xin Yan, Dan-Chen He, Ping Song, Tian-Qiong Shi

{"title":"Expansion of YALIcloneHR toolkit for Yarrowia lipolytica combined with Golden Gate and CRISPR technology","authors":"Qi Shen, Fang Yan, Ya-Wen Li, Jian Wang, Jia Ji, Wen-Xin Yan, Dan-Chen He, Ping Song, Tian-Qiong Shi","doi":"10.1007/s10529-023-03444-1","DOIUrl":"https://doi.org/10.1007/s10529-023-03444-1","url":null,"abstract":"Metabolic Engineering of yeast is a critical approach to improving the production capacity of cell factories. To obtain genetically stable recombinant strains, the exogenous DNA is preferred to be integrated into the genome. Previously, we developed a Golden Gate toolkit YALIcloneNHEJ, which could be used as an efficient modular cloning toolkit for the random integration of multigene pathways through the innate non-homologous end-joining repair mechanisms of Yarrowia lipolytica. We expanded the toolkit by designing additional building blocks of homologous arms and using CRISPR technology. The reconstructed toolkit was thus entitled YALIcloneHR and designed for gene-specific knockout and integration. To verify the effectiveness of the system, the gene PEX10 was selected as the target for the knockout. This system was subsequently applied for the arachidonic acid production, and the reconstructed strain can accumulate 4.8% of arachidonic acid. The toolkit will expand gene editing technology in Y. lipolytica, which would help produce other chemicals derived from acetyl-CoA in the future.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"38 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138552247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of d-glucaric acid with phosphoglucose isomerase-deficient Saccharomyces cerevisiae 利用磷酸葡萄糖异构酶缺陷的酿酒酵母生产 d-葡萄糖酸

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-08 DOI: 10.1007/s10529-023-03443-2

Mervi Toivari, Maija-Leena Vehkomäki, Laura Ruohonen, Merja Penttilä, Marilyn G. Wiebe

{"title":"Production of d-glucaric acid with phosphoglucose isomerase-deficient Saccharomyces cerevisiae","authors":"Mervi Toivari, Maija-Leena Vehkomäki, Laura Ruohonen, Merja Penttilä, Marilyn G. Wiebe","doi":"10.1007/s10529-023-03443-2","DOIUrl":"https://doi.org/10.1007/s10529-023-03443-2","url":null,"abstract":"d-Glucaric acid is a potential biobased platform chemical. Previously mainly Escherichia coli, but also the yeast Saccharomyces cerevisiae, and Pichia pastoris, have been engineered for conversion of d-glucose to d-glucaric acid via myo-inositol. One reason for low yields from the yeast strains is the strong flux towards glycolysis. Thus, to decrease the flux of d-glucose to biomass, and to increase d-glucaric acid yield, the four step d-glucaric acid pathway was introduced into a phosphoglucose isomerase deficient (Pgi1p-deficient) Saccharomyces cerevisiae strain. High d-glucose concentrations are toxic to the Pgi1p-deficient strains, so various feeding strategies and use of polymeric substrates were studied. Uniformly labelled 13C-glucose confirmed conversion of d-glucose to d-glucaric acid. In batch bioreactor cultures with pulsed d-fructose and ethanol provision 1.3 g d-glucaric acid L−1 was produced. The d-glucaric acid titer (0.71 g d-glucaric acid L−1) was lower in nitrogen limited conditions, but the yield, 0.23 g d-glucaric acid [g d-glucose consumed]−1, was among the highest that has so far been reported from yeast. Accumulation of myo-inositol indicated that myo-inositol oxygenase activity was limiting, and that there would be potential to even higher yield. The Pgi1p-deficiency in S. cerevisiae provides an approach that in combination with other reported modifications and bioprocess strategies would promote the development of high yield d-glucaric acid yeast strains.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"19 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138552336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kinetic and thermodynamic studies on the thermal inactivation of lipase immobilized on glutaraldehyde-activated rice husk silica 固定在戊二醛活化稻壳二氧化硅上的脂肪酶热失活的动力学和热力学研究

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-08 DOI: 10.1007/s10529-023-03449-w

Iara C. A. Bolina, Adriano A. Mendes

{"title":"Kinetic and thermodynamic studies on the thermal inactivation of lipase immobilized on glutaraldehyde-activated rice husk silica","authors":"Iara C. A. Bolina, Adriano A. Mendes","doi":"10.1007/s10529-023-03449-w","DOIUrl":"https://doi.org/10.1007/s10529-023-03449-w","url":null,"abstract":"The objective of this study was to obtain sufficient information on the thermal stabilization of a food-grade lipase from Thermomyces lanuginosus (TLL) using the immobilization technique. To do this, a new non-porous support was prepared via the sequential extraction of SiO2 from rice husks, followed by functionalization with (3-aminopropyl) triethoxysilane – 3-APTES (Amino–SiO2), and activation with glutaraldehyde – GA (GA-Amino-SiO2). We evaluated the influence of GA concentration, which varied from 0.25% v v−1 to 4% v v−1, on the immobilization parameters and enzyme thermal stabilization. The thermal inactivation parameters for both biocatalyst forms (soluble or immobilized TLL) were calculated by fitting a non-first-order enzyme inactivation kinetic model to the experimental data. According to the results, TLL was fully immobilized on the external support surface activated with different GA concentrations using an initial protein load of 5 mg g−1. A sharp decrease of hydrolytic activity was observed from 216.6 ± 12.4 U g−1 to 28.6 ± 0.9 U g−1 of after increasing the GA concentration from 0.25% v v−1 to 4.0% v v−1. The support that was prepared using a GA concentration at 0.5% v v−1 provided the highest stabilization of TLL – 31.6-times more stable than its soluble form at 60 °C. The estimations of the thermodynamic parameters, e.g., inactivation energy (Ed), enthalpy (ΔH#), entropy (ΔS#), and the Gibbs energy (ΔG#) values, confirmed the enzyme stabilization on the external support surface at temperatures ranging from 50 to 65 °C. These results show promising applications for this new heterogeneous biocatalyst in industrial processes given the high catalytic activity and thermal stability.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"1 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138552458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immobilization of rough morphotype Mycolicibacterium neoaurum R for androstadienedione production 固定粗糙形态的新牛磺酸霉菌 R 以生产雄甾二烯二酮

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-08 DOI: 10.1007/s10529-023-03448-x

Anqi Zhao, Yamei Li, Lixia Wu, Zhi Wang, Yongkun Lv, Wenlong Xiong, Mohammad Asraful Alam, Guohua Liu, Jingliang Xu

引用次数: 0

Enhancing suspended cell transfection by inducing localized distribution of the membrane actin cortex before exposure to electromechanical stimulation. 在暴露于机电刺激前，通过诱导肌动蛋白皮层膜的局部分布来增强悬浮细胞转染。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-01 Epub Date: 2023-09-01 DOI: 10.1007/s10529-023-03382-y

Wenjing Huang, Yibo Ma, Naotomo Tottori, Yoko Yamanishi

{"title":"Enhancing suspended cell transfection by inducing localized distribution of the membrane actin cortex before exposure to electromechanical stimulation.","authors":"Wenjing Huang, Yibo Ma, Naotomo Tottori, Yoko Yamanishi","doi":"10.1007/s10529-023-03382-y","DOIUrl":"10.1007/s10529-023-03382-y","url":null,"abstract":"Objectives: During physical transfection, an electrical field or mechanical force is used to induce cell transfection. We tested if the disruption of a dense actin layer underneath the membrane of a suspended cell enhances cell transfection.Results: A bubble generator was used to electromechanically stimulate suspended cells. To clarify the influence of the actin layer (the actin cortex) on cell transfection efficiency, we used an actin polymerization inhibitor (cytochalasin D) to disrupt the actin cortex before electromechanical stimulation. Without cytochalasin D treatment, signals from the overall actin cortex decreased after electromechanical stimulation. With cytochalasin D treatment, there was localized F-actin aggregation under static conditions. After electromechanical stimulation, there was a partial loss (localized disruption), but no overall disruption, of the actin cortex. With the pretreatment with cytochalasin D, the transfection efficiency of plasmids (4.7, 8.3, or 11 kbp) into NIH/3T3 or UMR-106 cells increased significantly after exposure to electromechanical stimulation.Conclusions: Localized distribution of the actin cortex before exposure to electromechanical stimulation is crucial for inducing a partial loss of the cortex, which improves transfection efficiency and large plasmid delivery.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"1417-1430"},"PeriodicalIF":2.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10503042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, identification, and preparation of tyrosinase inhibitory peptides from Pinctada martensii meat. 马氏珠母贝肉酪氨酸酶抑制肽的分离、鉴定和制备。

IF 2.7 4区生物学

Biotechnology Letters Pub Date : 2023-12-01 Epub Date: 2023-10-24 DOI: 10.1007/s10529-023-03437-0

Jinhao Meng, Jiaojiao Liu, Jing Lu, Pingyingzi Jiang, Yunxia Bai, Xiaoling Liu, Shubo Li

引用次数: 0