Nitrile hydratase as a promising biocatalyst: recent advances and future prospects

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2024-09-13 DOI:10.1007/s10529-024-03530-y

Chao Feng, Jing Chen, Wenxin Ye, Zhanshi Wang

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Abstract

Amides are an important type of synthetic intermediate used in the chemical, agrochemical, pharmaceutical, and nutraceutical industries. The traditional chemical process of converting nitriles into the corresponding amides is feasible but is restricted because of the harsh conditions required. In recent decades, nitrile hydratase (NHase, EC 4.2.1.84) has attracted considerable attention because of its application in nitrile transformation as a prominent biocatalyst. In this review, we provide a comprehensive survey of recent advances in NHase research in terms of natural distribution, enzyme screening, and molecular modification on the basis of its characteristics and catalytic mechanism. Additionally, industrial applications and recent significant biotechnology advances in NHase bioengineering and immobilization techniques are systematically summarized. Moreover, the current challenges and future perspectives for its further development in industrial applications for green chemistry were also discussed. This study contributes to the current state-of-the-art, providing important technical information for new NHase applications in manufacturing industries.

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腈水解酶作为一种前景广阔的生物催化剂：最新进展与未来展望

酰胺是化工、农用化学品、制药和保健品行业使用的一种重要合成中间体。将腈转化为相应酰胺的传统化学工艺是可行的，但由于所需的条件苛刻而受到限制。近几十年来，腈水解酶（NHase，EC 4.2.1.84）作为一种重要的生物催化剂在腈转化中的应用引起了广泛关注。在这篇综述中，我们将根据腈水解酶的特点和催化机理，从天然分布、酶筛选和分子修饰等方面全面介绍腈水解酶研究的最新进展。此外，我们还系统地总结了 NHase 生物工程和固定化技术的工业应用和最新重大生物技术进展。此外，还讨论了其在绿色化学工业应用中进一步发展的当前挑战和未来前景。本研究为当前最先进的技术做出了贡献，为 NHase 在制造业中的新应用提供了重要的技术信息。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.