The repertoire and levels of secondary metabolites in microbial cocultures depend on the inoculation ratio: a case study involving Aspergillus terreus and Streptomyces rimosus.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-06-06 DOI:10.1007/s10529-024-03500-4

Tomasz Boruta, Grzegorz Englart, Martyna Foryś, Weronika Pawlikowska

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Abstract

Objective: The aim of this study was to determine the influence of the inoculation volume ratio on the production of secondary metabolites in submerged cocultures of Aspergillus terreus and Streptomyces rimosus.

Results: The shake flask cocultures were initiated by using 23 inoculum variants that included different volumes of A. terreus and S. rimosus precultures. In addition, the axenic controls were propagated in parallel with the cocultures. UPLC‒MS analysis revealed the presence of 15 secondary metabolites, 12 of which were found both in the "A. terreus vs. S. rimosus" cocultures and axenic cultures of either A. terreus or S. rimosus. The production of the remaining 3 molecules was recorded solely in the cocultures. The repertoire and quantity of secondary metabolites were evidently dependent on the inoculation ratio. It was also noted that detecting filamentous structures resembling typical morphological forms of a given species was insufficient to predict the presence of a given metabolite.

Conclusions: The modification of the inoculation ratio is an effective strategy for awakening and enhancing the production of secondary metabolites that are not biosynthesized under axenic conditions.

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微生物共培养物中次生代谢物的种类和水平取决于接种比例：一项涉及赤曲霉和链霉菌的案例研究。

研究目的本研究的目的是确定接种量比对赤曲霉和菱形链霉菌浸没共培养产生次生代谢产物的影响：结果：使用 23 种不同的接种体进行摇瓶共培养，这些接种体包括不同体积的赤曲霉和轮状链霉菌预培养物。此外，在进行共培养的同时，还进行了轴向对照繁殖。超高效液相色谱-质谱（UPLC-MS）分析显示存在 15 种次级代谢物，其中 12 种既存在于 "赤霉菌 vs. 利莫索菌 "共培养物中，也存在于赤霉菌或利莫索菌的轴向培养物中。其余 3 种分子的产生仅在共培养物中有记录。次级代谢物的种类和数量显然取决于接种比例。此外还注意到，检测到与特定物种典型形态相似的丝状结构不足以预测特定代谢物的存在：结论：改变接种比例是唤醒和提高轴生条件下无法生物合成的次生代谢物产量的有效策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.