Biotechnology Letters最新文献_第9页

Toxicants improve glycerol production in the fermentation of undetoxified hydrolysate by Candida glycerinogenes. 在甘油酸根念珠菌发酵未解毒水解物的过程中，毒物可提高甘油产量。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-07-31 DOI: 10.1007/s10529-024-03503-1

Xiaohong Zhao, Hong Zong, Xinyao Lu, Bin Zhuge

{"title":"Toxicants improve glycerol production in the fermentation of undetoxified hydrolysate by Candida glycerinogenes.","authors":"Xiaohong Zhao, Hong Zong, Xinyao Lu, Bin Zhuge","doi":"10.1007/s10529-024-03503-1","DOIUrl":"10.1007/s10529-024-03503-1","url":null,"abstract":"Objectives: Toxicants inhibit microbial fermentation and reduce product titres. This work investigated the glycerol production characteristics of Candida glycerinogenes in highly toxic unwashed undetoxified hydrolysate and provided new ideas for high glycerol production from hydrolysates.Results: The unwashed hydrolysate contains higher concentrations of toxicants, such as furfural, acetic acid, phenols and NaCl than the washed alkali-treated bagasse hydrolysate. C. glycerinogenes fermented unwashed undetoxified hydrolysate yielded 36.1 g/L glycerol, 15.8% higher than the washed hydrolysate, suggesting that the toxicants stimulated glycerol synthesis. qRT-PCR analysis showed that toxicants of unwashed undetoxified hydrolysates greatly up-regulated the transcript levels of the genes GPD1, HXT4 and MSN4 et al. Overexpressing the above genes increased glycerol production by 27.9% to 46.1 g/L. And it was further increased by 8.8% to 50.1 g/L in a 5 L bioreactor.Conclusions: This result proves that toxicants in lignocellulosic hydrolysates can increase the titre of microbial glycerol production.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"1057-1068"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141858926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lentilactobacillus farraginis FSI (3): a whole cell biocatalyst for the synthesis of kojic acid derivative under aquatic condition. 远拉氏扁豆乳杆菌 FSI (3)：水生条件下合成曲酸衍生物的全细胞生物催化剂。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-08-20 DOI: 10.1007/s10529-024-03514-y

Mangal A Chaudhari, Pratiksha R Wankhede, Kiran S Dalal, Arun D Kale, Dipak S Dalal, Bhushan L Chaudhari

{"title":"Lentilactobacillus farraginis FSI (3): a whole cell biocatalyst for the synthesis of kojic acid derivative under aquatic condition.","authors":"Mangal A Chaudhari, Pratiksha R Wankhede, Kiran S Dalal, Arun D Kale, Dipak S Dalal, Bhushan L Chaudhari","doi":"10.1007/s10529-024-03514-y","DOIUrl":"10.1007/s10529-024-03514-y","url":null,"abstract":"Kojic acid derivatives are useful in the cosmetics and pharmaceutical industries. The current investigation focuses on the search for a safe and environmentally friendly newer whole-cell biocatalyst for the synthesis of kojic acid derivative especially 2-amino-6-(hydroxymethyl)-8-oxo-4-phenyl-4,8-dihydropyrano[3,2-b]pyran-3-carbonitrile (APhCN). In this context, a total of six cultures were isolated from fecal samples of infants and subjected to probiotic characterization followed by screening as whole cell biocatalyst (WCB). In this multicomponent reaction, benzaldehyde, malononitrile, and kojic acid were used to synthesize APhCN at room temperature under aqueous conditions. The screening of potent whole cell biocatalyst (WCB) from isolated cultures was done by comparing reaction time and percent yield. The potent WCB gave a good yield of 95% within 15 h of time and hence further characterized biochemically and identified as Lentilactobacillus farraginis by using 16S rRNA gene sequencing. Lactobacilli having GRAS (generally regarded as safe) status and being able to carry out this transformation under moderate reaction conditions with easy recovery of both product and biocatalyst, it has the potential to replace some of the chemical catalytic methods.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"1107-1120"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142003511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical characterization of a recombinant laccase from Halalkalibacterium halodurans C-125 and its application in the biotransformation of organic compounds. Halalkalibacterium halodurans C-125 重组漆酶的生化特征及其在有机化合物生物转化中的应用。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-10-28 DOI: 10.1007/s10529-024-03532-w

Jihene Maati, Jolanta Polak, Monika Janczarek, Marcin Grąz, Issam Smaali, Anna Jarosz-Wilkołazka

{"title":"Biochemical characterization of a recombinant laccase from Halalkalibacterium halodurans C-125 and its application in the biotransformation of organic compounds.","authors":"Jihene Maati, Jolanta Polak, Monika Janczarek, Marcin Grąz, Issam Smaali, Anna Jarosz-Wilkołazka","doi":"10.1007/s10529-024-03532-w","DOIUrl":"10.1007/s10529-024-03532-w","url":null,"abstract":"Objectives: This study aimed to produce an engineered recombinant laccase from extremophilic Halalkalibacterium halodurans C-125 (Lac-HhC-125) with higher protein yield, into a more active conformation and with properties that meet the fundamental needs of biotechnological application.Results: The rLac-HhC125 was partially purified by size exclusion chromatography and concentrated by ultrafiltration (10 kDa) with a yield of 57.6%. Oxidation reactions showed that adding 2 mM CuSO4 to the assay solution led to activating the laccase. To increase its initial activity, the rLac-HhC125 was treated at 50 °C for 20 min before the assays, improving its performance by fourfold using the syringaldazine as a substrate. When treated with EDTA, methanol, ethanol, and DMSO, the rLac-HhC125 maintained more than 80% of its original activity. Interestingly, the acetonitrile induced a twofold activity of the rLac-HhC125. The putative rLac-HhC125 demonstrated a capability of efficient transformation of different organic compounds at pH 6, known as dye precursors, into coloured molecules.Conclusion: The rLac-HhC125 was active at high temperatures and alkaline pH, exhibited tolerance to organic solvents, and efficiently transformed different hydroxy derivatives into coloured compounds, which indicates that it can be used in various biotechnological processes.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"1199-1218"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11550293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-yield magnetosome production of Magnetospirillum magneticum strain AMB-1 in flask fermentation through simplified processing and optimized iron supplementation. 通过简化处理和优化补铁，在烧瓶发酵过程中高产生产磁螺菌菌株 AMB-1 的磁小体。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-07-20 DOI: 10.1007/s10529-024-03507-x

Yu Wang, Zhengyi Liu, Wenjun Li, Hongli Cui, Yandi Huang, Song Qin

{"title":"High-yield magnetosome production of Magnetospirillum magneticum strain AMB-1 in flask fermentation through simplified processing and optimized iron supplementation.","authors":"Yu Wang, Zhengyi Liu, Wenjun Li, Hongli Cui, Yandi Huang, Song Qin","doi":"10.1007/s10529-024-03507-x","DOIUrl":"10.1007/s10529-024-03507-x","url":null,"abstract":"Objectives: Developing a simplified flask fermentation strategy utilizing magnetotactic bacterium AMB-1 and optimized iron supplementation for high-yield magnetosome production to address the challenges associated with magnetosome acquisition.Results: A reliable processing for the pure culture of AMB-1 was established using standard laboratory consumables and equipment. Subsequently, the medium and iron supplementation were optimized to enhance the yield of AMB-1 magnetosomes. The mSLM supported higher biomass accumulation in flask fermentation, reaching an OD565 of ~ 0.7. The premixed solution of ferric quinate and EDTA-Fe (at a ratio of 0.5:0.5 and a concentration of 0.4 mmol/L) stabilized Fe3+ and significantly increased the reductase activity of AMB-1. Flask fermentations with an initial volume of 15 L were then conducted employing the optimized fermentation strategy. After two rounds of iron and nutrient supplementation, the magnetosome yield reached 185.7 ± 9.5 mg/batch (approximately 12 mg/L), representing the highest AMB-1 flask fermentation yield to our knowledge.Conclusion: A flask fermentation strategy for high-yield magnetsome production was developed, eliminating the need for bioreactors and greatly simplifying the process of magnetosome acquisition.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"1069-1083"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141730993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A key component Rxt3 in the Rpd3L histone deacetylase complex regulates development, stress tolerance, amylase production and kojic acid synthesis in Aspergillus oryzae. Rpd3L 组蛋白去乙酰化酶复合物中的一个关键成分 Rxt3 调节黑曲霉的发育、抗逆性、淀粉酶的产生和曲酸的合成。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-07-31 DOI: 10.1007/s10529-024-03515-x

Chaofeng Chang, Herui Wang, Yiling Liu, Yiting Xie, Dingxiang Xue, Feng Zhang

引用次数: 0

Efficient genome engineering in Mycolicibacterium neoaurum using Cas9 from Streptococcus thermophilus. 利用嗜热链球菌的 Cas9 在新牛磺酸霉菌中进行高效基因组工程。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-07-31 DOI: 10.1007/s10529-024-03519-7

Gedan Xiang, Tao Liu, Lekai Li, Guihong Lin, Ke Liu, Fengqing Wang

{"title":"Efficient genome engineering in Mycolicibacterium neoaurum using Cas9 from Streptococcus thermophilus.","authors":"Gedan Xiang, Tao Liu, Lekai Li, Guihong Lin, Ke Liu, Fengqing Wang","doi":"10.1007/s10529-024-03519-7","DOIUrl":"10.1007/s10529-024-03519-7","url":null,"abstract":"Non-pathogenic mycobacteria, including Mycolicibacterium neoaurum, can directly utilize phytosterols for large-scale industrial production of steroid medicine intermediates due to their natural steroid metabolism pathway. The targeted genetic modification of M. neoaurum is conducive to the selection of high-yield engineering bacteria with high-value-added product, such as Pregnadien-20-carboxylic acid (PDC), which is an important precursor for synthesizing some corticosteroids. Based on heterologous type II CRISPR/sth1Cas9 system, a simple strategy was developed to genetic engineer M. neoaurum genome. Here, a customizable plasmid tool pMSC9 was constructed from pMV261 with integration of sth1Cas9 protein and corresponding sgRNA scaffold. Subsequently, the pMSC9 was inserted with spacer sequences corresponding to different targeted genes, generating editing plasmids, and then transformed into M. neoaurum. As a result, the targeted genes were introduced with DNA double stand breaks (DSBs) by CRISPR/sth1Cas9 system and then repaired by innate non-homologous end-joining (NHEJ) mechanism. Finally, editing plasmids were cured from correctly edited M. neoaurum mutants by means of no resistance cultivation, and the resulting mutant deleting the one target gene was used as the host to which another target gene could be deleted via the same process. This study demonstrated that the CRISPR/sth1Cas9 tool allowed M. neoaurum strains to be rapidly edited. And the editing mode of CRISPR/sth1Cas9 system indicated that this tool was an important supplement to the gene editing toolbox of M. neoaurum.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"1319-1332"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141854653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multilevel metabolic engineering for enhanced synthesis of S-adenosylmethionine by Bacillus amyloliquefaciens. 通过多级代谢工程提高淀粉样芽孢杆菌合成 S-腺苷蛋氨酸的能力。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-08-20 DOI: 10.1007/s10529-024-03523-x

Cong Jiang, Dian Zou, Liying Ruan, Wenyuan Han, Xuetuan Wei

引用次数: 0

Gallic acid as biofilm inhibitor can improve transformation efficiency of Ruminiclostridium papyrosolvens. 没食子酸作为生物膜抑制剂可提高瘤胃梭菌的转化效率。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-08-20 DOI: 10.1007/s10529-024-03522-y

Duodong Wang, Na Liu, Mingqiang Qiao, Chenggang Xu

引用次数: 0

Amyloidogenesis of SARS-CoV-2 delta plus and omicron variants receptor-binding domain (RBD): impact of SUMO fusion tag. SARS-CoV-2 delta plus 和 omicron 变体受体结合域 (RBD) 的淀粉样蛋白生成：SUMO 融合标签的影响。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-08-25 DOI: 10.1007/s10529-024-03525-9

Sadegh Zargan, Hasan Jalili, Bahareh Dabirmanesh, Saba Mesdaghinia, Khosro Khajeh

{"title":"Amyloidogenesis of SARS-CoV-2 delta plus and omicron variants receptor-binding domain (RBD): impact of SUMO fusion tag.","authors":"Sadegh Zargan, Hasan Jalili, Bahareh Dabirmanesh, Saba Mesdaghinia, Khosro Khajeh","doi":"10.1007/s10529-024-03525-9","DOIUrl":"10.1007/s10529-024-03525-9","url":null,"abstract":"Purpose: The RBD of SARS-CoV-2 mediates viral entry into host cells by binding to the host receptor ACE2. SARS-CoV-2 infection is linked to various health issues resembling amyloid-related problems, persuading us to investigate the amyloidogenicity of the SARS-CoV-2 spike RBD.Methods: The FoldAmyloid program was used to assess the amyloidogenic propensities in the RBD of Delta Plus and RBD of the Omicron variant, with and without the SUMO tag. After the expression of RBDs, purification, and dialysis steps were performed, subsequently the ThT assay, FTIR, and TEM were employed to check the RBD ability to form fibrils.Results: The ThT assay, TEM, and FTIR revealed the ability of RBD to self-assemble into β-sheet-rich aggregates (48.4% β-sheet content). Additionally, the presence of the SUMO tag reduced the formation of RBD amyloid-like fibrils. The amyloidogenic potential of Omicron RBD was higher than Delta Plus, according to both in silico and experimental analyses.Conclusions: The SARS-CoV-2 RBD can assemble itself by forming aggregates containing amyloid-like fibrils and the presence of a SUMO tag can significantly decrease the formation of RBD amyloid-like fibrils. In silico analysis suggested that variation in the ThT fluorescence intensity of amyloid accumulations in the two SARS-CoV-2 strains arises from specific mutations in their RBD regions.","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":" ","pages":"1037-1048"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142054858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombinant expression, downstream optimization, and therapeutic evaluation of recombinant human interleukin-37 for cancer therapy. 用于癌症治疗的重组人白细胞介素-37 的重组表达、下游优化和治疗评估。

IF 2 4区生物学

Biotechnology Letters Pub Date : 2024-12-01 Epub Date: 2024-10-18 DOI: 10.1007/s10529-024-03539-3

Zaheer Abbas, Samia Afzal, Nao Akusa Fujimura, Muhammad Akram, Saad Tahir, Kausar Malik, Nadeem Ahmed

引用次数: 0