High-yield magnetosome production of Magnetospirillum magneticum strain AMB-1 in flask fermentation through simplified processing and optimized iron supplementation.

IF 2 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Yu Wang, Zhengyi Liu, Wenjun Li, Hongli Cui, Yandi Huang, Song Qin
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Abstract

Objectives: Developing a simplified flask fermentation strategy utilizing magnetotactic bacterium AMB-1 and optimized iron supplementation for high-yield magnetosome production to address the challenges associated with magnetosome acquisition.

Results: A reliable processing for the pure culture of AMB-1 was established using standard laboratory consumables and equipment. Subsequently, the medium and iron supplementation were optimized to enhance the yield of AMB-1 magnetosomes. The mSLM supported higher biomass accumulation in flask fermentation, reaching an OD565 of ~ 0.7. The premixed solution of ferric quinate and EDTA-Fe (at a ratio of 0.5:0.5 and a concentration of 0.4 mmol/L) stabilized Fe3+ and significantly increased the reductase activity of AMB-1. Flask fermentations with an initial volume of 15 L were then conducted employing the optimized fermentation strategy. After two rounds of iron and nutrient supplementation, the magnetosome yield reached 185.7 ± 9.5 mg/batch (approximately 12 mg/L), representing the highest AMB-1 flask fermentation yield to our knowledge.

Conclusion: A flask fermentation strategy for high-yield magnetsome production was developed, eliminating the need for bioreactors and greatly simplifying the process of magnetosome acquisition.

Abstract Image

通过简化处理和优化补铁,在烧瓶发酵过程中高产生产磁螺菌菌株 AMB-1 的磁小体。
目标:开发一种简化的烧瓶发酵策略,利用磁生细菌 AMB-1 和优化的铁补充剂生产高产磁小体:开发一种简化的烧瓶发酵策略,利用磁诱导细菌 AMB-1 和优化的铁补充剂进行高产磁小体生产,以应对与磁小体获取相关的挑战:结果:利用标准实验室耗材和设备建立了可靠的 AMB-1 纯培养过程。随后,对培养基和补铁进行了优化,以提高 AMB-1 磁小体的产量。在烧瓶发酵过程中,mSLM 支持较高的生物量积累,OD565 达到约 0.7。奎宁酸铁和 EDTA-Fe(比例为 0.5:0.5,浓度为 0.4 mmol/L)的预混合溶液稳定了 Fe3+,并显著提高了 AMB-1 的还原酶活性。然后,采用优化的发酵策略进行了初始容积为 15 升的烧瓶发酵。经过两轮铁和营养物质补充后,磁小体产量达到 185.7 ± 9.5 mg/批(约 12 mg/L),这是我们所知的最高 AMB-1 烧瓶发酵产量:结论:开发出了一种用于高产磁小体生产的瓶式发酵策略,省去了生物反应器,大大简化了磁小体的获取过程。
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来源期刊
Biotechnology Letters
Biotechnology Letters 工程技术-生物工程与应用微生物
CiteScore
5.90
自引率
3.70%
发文量
108
审稿时长
1.2 months
期刊介绍: Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.
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