Enhancing terpenoid production in Saccharomyces cerevisiae via cell morphology engineering.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2025-05-17 DOI:10.1007/s10529-025-03584-6

Ke Bi, Siqi Zuo, Jiazhang Lian, Zhinan Xu

引用次数: 0

Abstract

There is a growing interest in establishing yeast as a cell factory for terpenoid production. Given the hydrophobicity of many terpenoids, expanding the intracellular storage pool is an effective strategy to improve titers. In this study, the storage capacity was enhanced by increasing yeast cell size through the knockout of EST1 and SSN8. The influence on lycopene and 7-dehydrocholesterol production were then evaluated. The results demonstrated that EST1 and SSN8 deletion resulted in 4.27- and 3.45-fold improvement on lycopene production, and 1.99- and 1.2-fold improvement on 7-dehydrocholesterol production, respectively. These findings suggest cell enlargement as a promising strategy for enhancing terpenoid production in yeast.

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利用细胞形态学工程提高酿酒酵母萜类化合物的产量。

人们对将酵母作为生产萜类化合物的细胞工厂越来越感兴趣。鉴于许多萜类化合物的疏水性，扩大细胞内储存池是提高滴度的有效策略。在本研究中，通过敲除EST1和SSN8来增加酵母细胞大小，从而提高了存储容量。然后评估了对番茄红素和7-脱氢胆固醇产量的影响。结果表明，EST1和SSN8缺失导致番茄红素产量分别提高4.27倍和3.45倍，7-脱氢胆固醇产量分别提高1.99倍和1.2倍。这些发现表明，细胞扩大是一种很有希望的提高酵母萜类化合物生产的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.