Bacillus alcalophilus RecJ (BaRecJ) can drive the adaptive evolution of S. cerevisiae.

IF 2.1 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2025-07-22 DOI:10.1007/s10529-024-03552-6

Jixiang Shang, Yanchao Zhang, Zongjun Xu, Shouqing Zhang, Zhongtao Sun, Minggang Zheng

引用次数: 0

Abstract

With the continuous advancement of technologies such as microbial cultivation, DNA sequencing, bioinformatics, and genetic engineering, in vivo mutagenesis methods based on perturbation factors are now widely utilized. We identified a RecJ enzyme (BaRecJ) with endonuclease and exonuclease activities from Bacillus alcalophilus, and established a broad-spectrum mutagenic method based on the endonuclease and exonuclease activities of BaRecJ. The BaRecJ mutagenesis method was applied to S cerevisiae to enhance its ethanol or acetic acid tolerance, resulting in mutant strains with improved fermentation performance. Genomic resequencing analysis summarized genes possibly associated with the tolerance of mutants. BaRecJ mutagenesis method not only holds immense potential in microbial mutagenesis breeding and adaptive evolution but also, when coupled with genomic resequencing, allows for the rapid identification of candidate genetic loci associated with specific traits.

查看原文本刊更多论文

嗜钙芽孢杆菌RecJ （BaRecJ）可以驱动酿酒酵母的适应性进化。

随着微生物培养、DNA测序、生物信息学、基因工程等技术的不断进步，基于微扰因子的体内诱变方法得到了广泛的应用。从嗜钙芽孢杆菌中鉴定出具有内切酶和外切酶活性的RecJ酶（BaRecJ），并基于其内切酶和外切酶活性建立了广谱诱变方法。采用BaRecJ诱变方法对酿酒酵母的乙醇或醋酸耐受性进行了增强，获得了发酵性能较好的突变菌株。基因组重测序分析总结了可能与突变体耐受性相关的基因。BaRecJ诱变方法不仅在微生物诱变育种和适应性进化方面具有巨大的潜力，而且当与基因组重测序相结合时，可以快速识别与特定性状相关的候选遗传位点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.