Characterization of the pesticidal crystal toxin protein Cry11Aa from Bacillus thuringiensis serovar israelensis VCRC B646 for mosquito larvae control.
IF 2.1 4区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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引用次数: 0
Abstract
Effective mosquito control is essential for reducing the transmission of vector-borne diseases. This study focuses on the comprehensive characterization of mosquitocidal toxins produced by Bacillus thuringiensis serovar israelensis (Bti) VCRC B646 and the associated insecticidal genes. The bacterium was cultured, and the spore-crystal complex was purified to identify the mosquitocidal proteins. The mosquitocidal toxins produced by the isolate were evaluated against Aedes(Ae.) aegypti and Culex(Cx.) quinquefasciatus. SDS-PAGE and LC-MS were conducted to analyse the toxin, and PCR was performed to amplify the toxin gene. Toxicity bioassays indicated lethal concentrations (LC50 and LC90) for Ae. aegypti (0.0022 mg/L and 0.004 mg/L), and Cx, quinquefasciatus (0.0025 mg/L and 0.0044 mg/L). SDS-PAGE and LC-MS analysis revealed that Cry11Aa5 (Pesticidal Crystal Protein) is the predominant toxin produced by this strain. PCR amplification confirmed the presence of genes encoding various insecticidal proteins, including Cry and Cyt toxins. Phylogenetic analysis was performed to assess the genetic relatedness and toxin profiles of the bacterial isolate. This detailed characterization of Bti VCRC B646 highlights its potential as a promising biopesticide candidate for mosquito control, contributing to the development of sustainable and eco-friendly strategies for vector management.
期刊介绍：
Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them.
All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included.
Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields.
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Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.
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