{"title":"苏云金芽孢杆菌（Bacillus thuringiensis serovar israelensis） VCRC B646杀虫晶体毒素蛋白Cry11Aa的鉴定","authors":"Abhisubesh Vijayakumar, Sahadiya Mandodan, Jibi Lukose, Aneha Kunnikuruvan, Kakhuangailiu Gangmei, Bhagyashree Bora, Hemaladkshmi Padmanaban, Mathivanan Ashokkumar, Subbiah Poopathi","doi":"10.1007/s10529-025-03640-1","DOIUrl":null,"url":null,"abstract":"<p><p>Effective mosquito control is essential for reducing the transmission of vector-borne diseases. This study focuses on the comprehensive characterization of mosquitocidal toxins produced by Bacillus thuringiensis serovar israelensis (Bti) VCRC B646 and the associated insecticidal genes. The bacterium was cultured, and the spore-crystal complex was purified to identify the mosquitocidal proteins. The mosquitocidal toxins produced by the isolate were evaluated against Aedes(Ae.) aegypti and Culex(Cx.) quinquefasciatus. SDS-PAGE and LC-MS were conducted to analyse the toxin, and PCR was performed to amplify the toxin gene. Toxicity bioassays indicated lethal concentrations (LC<sub>50</sub> and LC<sub>90</sub>) for Ae. aegypti (0.0022 mg/L and 0.004 mg/L), and Cx, quinquefasciatus (0.0025 mg/L and 0.0044 mg/L). SDS-PAGE and LC-MS analysis revealed that Cry11Aa5 (Pesticidal Crystal Protein) is the predominant toxin produced by this strain. PCR amplification confirmed the presence of genes encoding various insecticidal proteins, including Cry and Cyt toxins. Phylogenetic analysis was performed to assess the genetic relatedness and toxin profiles of the bacterial isolate. This detailed characterization of Bti VCRC B646 highlights its potential as a promising biopesticide candidate for mosquito control, contributing to the development of sustainable and eco-friendly strategies for vector management.</p>","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"47 5","pages":"100"},"PeriodicalIF":2.1000,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characterization of the pesticidal crystal toxin protein Cry11Aa from Bacillus thuringiensis serovar israelensis VCRC B646 for mosquito larvae control.\",\"authors\":\"Abhisubesh Vijayakumar, Sahadiya Mandodan, Jibi Lukose, Aneha Kunnikuruvan, Kakhuangailiu Gangmei, Bhagyashree Bora, Hemaladkshmi Padmanaban, Mathivanan Ashokkumar, Subbiah Poopathi\",\"doi\":\"10.1007/s10529-025-03640-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Effective mosquito control is essential for reducing the transmission of vector-borne diseases. This study focuses on the comprehensive characterization of mosquitocidal toxins produced by Bacillus thuringiensis serovar israelensis (Bti) VCRC B646 and the associated insecticidal genes. The bacterium was cultured, and the spore-crystal complex was purified to identify the mosquitocidal proteins. The mosquitocidal toxins produced by the isolate were evaluated against Aedes(Ae.) aegypti and Culex(Cx.) quinquefasciatus. SDS-PAGE and LC-MS were conducted to analyse the toxin, and PCR was performed to amplify the toxin gene. Toxicity bioassays indicated lethal concentrations (LC<sub>50</sub> and LC<sub>90</sub>) for Ae. aegypti (0.0022 mg/L and 0.004 mg/L), and Cx, quinquefasciatus (0.0025 mg/L and 0.0044 mg/L). SDS-PAGE and LC-MS analysis revealed that Cry11Aa5 (Pesticidal Crystal Protein) is the predominant toxin produced by this strain. PCR amplification confirmed the presence of genes encoding various insecticidal proteins, including Cry and Cyt toxins. Phylogenetic analysis was performed to assess the genetic relatedness and toxin profiles of the bacterial isolate. This detailed characterization of Bti VCRC B646 highlights its potential as a promising biopesticide candidate for mosquito control, contributing to the development of sustainable and eco-friendly strategies for vector management.</p>\",\"PeriodicalId\":8929,\"journal\":{\"name\":\"Biotechnology Letters\",\"volume\":\"47 5\",\"pages\":\"100\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2025-09-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biotechnology Letters\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s10529-025-03640-1\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology Letters","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s10529-025-03640-1","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Characterization of the pesticidal crystal toxin protein Cry11Aa from Bacillus thuringiensis serovar israelensis VCRC B646 for mosquito larvae control.
Effective mosquito control is essential for reducing the transmission of vector-borne diseases. This study focuses on the comprehensive characterization of mosquitocidal toxins produced by Bacillus thuringiensis serovar israelensis (Bti) VCRC B646 and the associated insecticidal genes. The bacterium was cultured, and the spore-crystal complex was purified to identify the mosquitocidal proteins. The mosquitocidal toxins produced by the isolate were evaluated against Aedes(Ae.) aegypti and Culex(Cx.) quinquefasciatus. SDS-PAGE and LC-MS were conducted to analyse the toxin, and PCR was performed to amplify the toxin gene. Toxicity bioassays indicated lethal concentrations (LC50 and LC90) for Ae. aegypti (0.0022 mg/L and 0.004 mg/L), and Cx, quinquefasciatus (0.0025 mg/L and 0.0044 mg/L). SDS-PAGE and LC-MS analysis revealed that Cry11Aa5 (Pesticidal Crystal Protein) is the predominant toxin produced by this strain. PCR amplification confirmed the presence of genes encoding various insecticidal proteins, including Cry and Cyt toxins. Phylogenetic analysis was performed to assess the genetic relatedness and toxin profiles of the bacterial isolate. This detailed characterization of Bti VCRC B646 highlights its potential as a promising biopesticide candidate for mosquito control, contributing to the development of sustainable and eco-friendly strategies for vector management.
期刊介绍：
Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them.
All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included.
Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields.
The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories.
Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.
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