Exploring the diversity of produced water bacteria with hydrocarbon-degrading potential using MALDI-TOF MS and multivariate statistical analyses.

IF 2.1 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2025-09-26 DOI:10.1007/s10529-025-03641-0

Maryam Al-Kaabi, Nabil Zouari, Mohammad Yousaf Ashfaq, Mohammad A Al-Ghouti

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引用次数: 0

Abstract

The success of bioremediation of produced water relies on the use of hydrocarbon-degrading bacteria. Hence, the selection of highly tolerant endogenous strains from produced water is crucial to designing successful bioremediation. However, the employed isolation and screening approaches are, in general, long. Integrative and rapid approaches based on microbiological and molecular techniques are now required due to the frequent fluctuation of the composition of the produced water. Here, enrichment cultures at high toxicity followed by protein profiling using MALDI-TOF MS were shown to be efficient in clustering the endogenous hydrocarbon-degrading bacteria and help select the potential candidates. Several bacterial strains (n = 18) were isolated from produced water sampled from Qatar's North Field natural gas production. Fourteen strains were identified as Bacillus cereus (n = 14), and one as Staphylococcus hominis (n = 1) using MALDI-TOF MS. Three strains were identified as Aneurinibacillus humi (n = 2) and Aneurinibacillus aneurinilyticus (n = 1) through ribotyping. The strains were further differentiated based on their protein profiles using MALDI-TOF MS and multivariate statistical analyses. Multivariate analyses (composite correlation index, principal component analysis, and dendrogram) demonstrated substantial diversity among the isolates, highlighting their potential as robust candidates for bioremediation and produced water treatment.

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利用MALDI-TOF质谱和多元统计分析，探索具有烃降解潜力的采出水细菌的多样性。

采出水生物修复的成功依赖于烃类降解菌的使用。因此，从产出水中选择高耐受性的内源菌株是设计成功的生物修复方法的关键。然而，通常采用的隔离和筛选方法耗时较长。由于采出水成分的频繁波动，现在需要基于微生物和分子技术的综合快速方法。在这里，高毒性富集培养，然后使用MALDI-TOF MS进行蛋白质分析，被证明可以有效地聚类内源性烃降解细菌，并有助于选择潜在的候选细菌。从卡塔尔北部气田开采的采出水中分离出数株细菌（n = 18）。MALDI-TOF ms鉴定出蜡样芽孢杆菌14株，人型葡萄球菌1株，分别为蜡样芽孢杆菌14株和人型葡萄球菌1株。利用MALDI-TOF MS和多元统计分析进一步区分菌株的蛋白质谱。多变量分析（复合相关指数、主成分分析和树形图）显示了分离物之间的巨大多样性，突出了它们作为生物修复和采出水处理的强大候选物的潜力。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.